A corn tissue culture device
By designing an open corn tissue culture equipment, the circulation and uniform contact of the culture medium is achieved using multiple segmented spaces and the second tube body, the problems of high operation difficulty, high cost and damage to plant seedlings are solved, and efficient and low-cost tissue culture is achieved.
Patent Information
- Application Number
- CN202411973187.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2044-12-30
AI Technical Summary
The existing corn tissue culture equipment is difficult to operate, costly and easily damage the root system of plant seedlings due to the narrow mouth of the glass bottle and the deep inner wall of the bottle.
A corn tissue culture equipment was designed, which was designed to facilitate cleaning with an open design, including a shell and a seedling tray. There were multiple division spaces and a second tube body in the shell. There were permeable holes and water absorption blocks at the bottom of the cultivation tank to realize circulation and uniform contact of the culture medium.
It reduces the cost of plant tissue culture, reduces the damage to plant seedlings, realizes efficient circulation and uniform soaking of the culture medium, and reduces water evaporation and leaf vitrification.
Smart Images
Figure CN119422897B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of tissue culture, and particularly to a maize tissue culture device. Background Art
[0002] In plant tissue culture, agar semi-solid medium or solid medium is mostly used. The agar medium provides nutrients for explants or plant seedlings, and has the advantages of simple operation, efficient propagation, and being conducive to the long-term preservation of high-quality germplasm.
[0003] In plant tissue culture, glass bottles are common culture containers, which have the advantages of high transparency, reusability, and high temperature resistance. However, the bottleneck of the glass bottle is narrow and the inner wall of the bottle is relatively deep. Special bottle brushes must be used to scrub repeatedly, which increases the operation difficulty and workload. The growth of plants will continuously consume the nutrients in the agar. Limited by the size of the glass bottle, it is generally necessary to replace the new agar medium every 3 to 6 weeks, which requires a large amount of manual input, increases the cost of plant tissue culture, and it is easy to damage the roots of plant seedlings when replacing the agar medium. Summary of the Invention
[0004] The technical problem to be solved by the present invention is to overcome the existing defects, and provide a maize tissue culture device, which is open for convenient cleaning, reduces the cost of plant tissue culture, and does not need to move plant seedlings during the tissue culture process, reducing the damage to plant seedlings.
[0005] To achieve the above object, the present invention provides the following technical solution: A maize tissue culture device, including a housing and a seedling-raising tray. A receiving space is opened on the top side of the housing. A plurality of partition plates are fixed along the length direction of the housing at the bottom side of the receiving space, and the partition plates divide the lower half of the receiving space into a plurality of divided spaces. A second tube body is penetrated through each partition plate, and both ends of the second tube body are bent downward.
[0006] The seedling-raising tray is located in the receiving space. The seedling-raising tray includes a plurality of cultivation housings arranged along the length direction of the housing and fixedly connected together at the top side. A plurality of cultivation grooves are opened on the top side of each cultivation housing. A plurality of liquid passing holes are opened at the bottom side of each cultivation groove. A cultivation housing is inserted into each divided space. A cover body that can be opened and closed is installed on the top side of the housing.
[0007] Among them, add culture solution into at least one divided space, put explants in the cultivation grooves, then the cover body completely covers the receiving space, move one end of the housing in the length direction upward to make the included angle between the housing and the horizontal plane reach a set angle. At this time, the liquid level of the culture solution in the divided space is higher than the second tube body with a lower height in this divided space.
[0008] Among them, the housing rotates periodically back and forth by a set angle around the width direction of the housing, so that the culture solution moves back and forth along the length direction of the housing.
[0009] As a preferred technical solution of the present invention, a water-absorbing block that blocks the liquid holes is placed at the bottom of each cultivation tank.
[0010] As a preferred technical solution of the present invention, the edge of the seedling tray is slidably and sealingly attached to the inner wall of the accommodating space. The seedling tray closes each partition space, and exhaust holes are provided at both ends in the length direction of the seedling tray. Air purification components are installed on both exhaust holes.
[0011] The air purification component includes a first pipe body. An installation hole is provided on the housing, and a second air filtration device is installed on the outer side of the installation hole. The inner side of the installation hole communicates with one end of the first pipe body, and the other end of the first pipe body communicates with the top side of the exhaust hole.
[0012] As a preferred technical solution of the present invention, a blocking cloth for adjusting the air flow resistance inside the second air filtration device is installed on at least one second air filtration device.
[0013] As a preferred technical solution of the present invention, an air exchange hole is provided on the cover body, and a first air filtration device is installed on the outer side of the air exchange hole.
[0014] As a preferred technical solution of the present invention, two second pipe bodies are installed on the partition board. One end of the second pipe body is close to the bottom of the partition board, and the other end of the second pipe body extends away from the bottom of the partition board with an extension pipe. The extension pipes of the two second pipe bodies are located on both sides of the partition board.
[0015] As a preferred technical solution of the present invention, a clamping groove is provided on the top side of the partition board, and a clamping block is detachably installed in the clamping groove. Both ends of the second pipe body penetrate through the clamping block and extend outside the clamping block.
[0016] As a preferred technical solution of the present invention, it further includes a base located below the housing. The top side of the base is rotatably connected to the bottom side of the connecting frame, and the connecting frame is fixed to the bottom side of the housing. A driving mechanism for driving the housing to rotate around the width direction of the housing is installed on the base.
[0017] As a preferred technical solution of the present invention, the cover body is made of transparent glass material or transparent plastic material, and the housing is made of transparent glass material or transparent plastic material.
[0018] As a preferred technical solution of the present invention, the set angle between the housing and the horizontal plane is 5° - 40°.
[0019] Compared with the prior art, the beneficial effects of the present invention are:
[0020] 1. In the corn tissue culture equipment of the present invention example, the housing rotates periodically and reciprocally by a set angle around the width direction of the housing, causing the culture solution to move reciprocally along the length direction of the housing. The culture solution stays in one of the partition spaces and then flows to an adjacent and lower-positioned partition space. The culture solution flows through each partition space in sequence, and the culture solution flows into the cultivation tank through the liquid passing holes, realizing the contact between the culture solution and the explant or the roots of the plant seedlings, and intermittently soaking the explant or the roots of the plant seedlings with the culture solution: On the one hand, the culture solution is used to provide nutrients for the explant or the plant seedlings, reducing manual input and the cost of plant tissue culture. During the tissue culture process, the plant seedlings do not need to be moved, reducing the damage to the plant seedlings. Moreover, the culture solution soaks the roots of the explants or plant seedlings at different positions in segments, and this equipment consumes less culture solution; On the other hand, only part of the partition spaces in the housing are filled with the culture solution, and the area of the liquid surface of the culture solution is small, reducing the water evaporation of the culture solution and reducing the vitrification of the leaves of the cultivated plant seedlings; On the further hand, during the periodic and reciprocal rotation of the housing around the width direction of the housing, the culture solution moving reciprocally along the length direction of the housing realizes the air exchange inside and outside the housing, reducing the harmful gases inside the housing and ensuring the normal respiration of the plant seedlings in tissue culture.
[0021] 2. In the corn tissue culture equipment of the present invention example, the air flow resistance inside the second air filtration device is adjusted by adding or removing the shielding cloth on the second air filtration device, thereby adjusting the contact time between the explant or the roots of the plant seedlings and the culture solution, ensuring sufficient contact between the explant or the roots of the plant seedlings and the culture solution, and improving the reliability of this corn tissue culture equipment.
[0022] 3. In the corn tissue culture equipment of the present invention example, a water-absorbing block that shields the liquid passing holes is placed at the bottom of each cultivation tank. The water-absorbing block adsorbs the culture solution, increasing the contact time between the explant or the roots of the plant seedlings and the culture solution.
[0023] 4. In the corn tissue culture equipment of the present invention example, the extension pipes of the two second pipe bodies are located on both sides of the partition board, ensuring the siphon effect of the second pipe body on the culture solution. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 is the front view structural schematic diagram of the present invention;
[0025] Figure 2 is the exploded structural schematic diagram of the present invention;
[0026] Figure 3 is the partial sectional view structural schematic diagram of the housing of the present invention;
[0027] Figure 4 is the top view structural schematic diagram of the seedling-raising tray of the present invention;
[0028] Figure 5Schematic upward view structure of the seedling-raising tray of the present invention;
[0029] Figure 6 Schematic inclined state structure of the housing and the seedling-raising tray of the present invention;
[0030] Figure 7 Schematic top view structure of the housing of the present invention.
[0031] In the figure: 1 cover body, 2 first air filtration device, 3 second air filtration device, 4 housing, 5 drive mechanism, 6 base, 7 seedling-raising tray, 8 first pipe body, 9 second pipe body, 10 partition board, 11 water absorption block, 12 exhaust hole, 13 liquid passing hole. Detailed implementation manners
[0032] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0033] Embodiment 1:
[0034] Please refer to Figures 1-7 , this embodiment discloses a corn tissue culture device, including a housing 4 and a seedling-raising tray 7. A receiving space is opened on the top side of the housing 4. A plurality of partition boards 10 are fixedly arranged along the length direction of the housing 4 at the bottom side of the receiving space, and the partition boards 10 divide the lower half of the receiving space into several divided spaces. A second pipe body 9 is penetrated through each partition board 10, and both ends of the second pipe body 9 are bent downward.
[0035] The seedling-raising tray 7 is located in the receiving space. The seedling-raising tray 7 includes a plurality of cultivation shells arranged along the length direction of the housing 4 and fixedly connected together at the top side. A plurality of cultivation grooves are opened on the top side of each cultivation shell, and a plurality of liquid passing holes 13 communicating with the divided spaces are opened at the bottom side of each cultivation groove. A cultivation shell is inserted into each divided space, and an openable and closable cover body 1 is installed on the top side of the housing 4.
[0036] The working process and principle of this embodiment are as follows:
[0037] The operator removes the cover body 1 and the seedling-raising tray 7 from the housing 4, and the operator uses an external sterilization device to sterilize the cover body 1, the seedling-raising tray 7 and the housing 4. The operator adds a culture solution into at least one divided space on a sterile operating table, and then the operator puts the seedling-raising tray 7 into the receiving space of the housing 4. The operator puts explants into each cultivation groove, and the operator makes the cover body 1 completely cover the receiving space.
[0038] The operator moves one end of the housing 4 in the length direction upward to make the angle between the housing 4 and the horizontal plane reach the set angle. At this time, the liquid level of the culture solution in the partition space is higher than the second tube 9 with a lower height in the partition space. The culture solution in this partition space flows into the adjacent and lower partition space through the second tube 9, and a siphon phenomenon occurs to the culture solution at the second tube 9.
[0039] The operator makes the housing 4 rotate periodically back and forth by a set angle around the width direction of the housing 4, so that the culture solution moves back and forth along the length direction of the housing 4. After staying in one of the partition spaces, the culture solution flows to the adjacent and lower partition space. The culture solution flows through each partition space in turn, and the culture solution flows into the cultivation tank through the liquid passing hole 13, realizing the contact between the culture solution and the explant or the root of the plant seedling, and making the explant or the root of the plant seedling be intermittently immersed in the culture solution.
[0040] This corn tissue culture equipment is convenient to clean, uses the culture solution to provide nutrients for the explant or the plant seedling, reduces manual input, and lowers the cost of plant tissue culture. During the tissue culture process, the plant seedling does not need to be moved, reducing the damage to the plant seedling. Moreover, the culture solution immerses the roots of the explants or plant seedlings at different positions in different time periods, and this equipment consumes less culture solution.
[0041] Furthermore, only part of the partition space in the housing 4 is filled with the culture solution, and the area of the liquid level of the culture solution is small, reducing the water evaporation of the culture solution and lowering the vitrification of the leaves of the cultivated plant seedlings.
[0042] Furthermore, as Figure 6 shown, a switching valve communicating with the accommodation space is installed on the housing 4, and the operator can replace the culture solution in the accommodation space through the switching valve. The culture solution of this equipment is convenient to replace.
[0043] Furthermore, the set angle of the angle between the housing 4 and the horizontal plane is 5° - 40°.
[0044] Furthermore, by replacing the cover 1 with different heights, this equipment can be adapted to corn seedlings of different heights.
[0045] Furthermore, the components of the culture solution include water, inorganic salts, carbohydrates, nitrogen-containing substances, and plant hormones.
[0046] Example Two:
[0047] As Figure 4 shown, this example discloses a corn tissue culture equipment, whose structure is substantially the same as that of Example One. The difference is that a water-absorbing block 11 covering the liquid passing hole 13 is placed at the bottom of each cultivation tank in this example, and the water-absorbing block 11 is a sponge or a cloth strip.
[0048] The working process and principle of this embodiment are as follows:
[0049] The water-absorbing block 11 adsorbs the culture solution, increasing the contact time between the explant or the root of the plant seedling and the culture solution.
[0050] Embodiment Three:
[0051] As Figure 2 , Figure 4 , Figure 6 shown, this embodiment discloses a corn tissue culture device, whose structure is substantially the same as that of the second embodiment. The difference is that the edge of the seedling-raising tray 7 in this embodiment is slidably and sealingly attached to the inner wall of the accommodating space. The seedling-raising tray 7 closes each divided space. Exhaust holes 12 are provided at both ends in the length direction of the seedling-raising tray 7, and air purification components are installed on both exhaust holes 12.
[0052] The air purification component includes a first pipe body 8. An installation hole is provided on the housing 4, and a second air filtering device 3 is installed on the outside of the installation hole. The inside of the installation hole is communicated with one end of the first pipe body 8, and the other end of the first pipe body 8 is communicated with the top side of the exhaust hole 12.
[0053] The working process and principle of this embodiment are as follows:
[0054] The air or culture solution in two adjacent divided spaces can flow through the second pipe body 9.
[0055] The operator rotates the housing 4 periodically and reciprocally by a set angle around the width direction of the housing 4, causing the culture solution to reciprocally move along the length direction of the housing 4. The culture solution divides the divided space into two non-connected groups, and the two groups of divided spaces are the group of divided spaces at a higher position and the group of divided spaces at a lower position. After staying in one of the divided spaces, the culture solution flows to an adjacent divided space at a lower position.
[0056] The volume of the group of divided spaces at a higher position becomes larger. The water-absorbing block 11 prevents the air above the seedling-raising tray 7 from entering the group of divided spaces at a higher position, the air pressure in the group of divided spaces at a higher position decreases, and the group of divided spaces at a higher position inhales air through the air purification component.
[0057] The volume of the group of divided spaces at a lower position becomes smaller, causing the air pressure in the group of divided spaces at a lower position to increase. The air in the group of divided spaces at a lower position pushes open the water-absorbing block 11 and enters the space between the seedling-raising tray 7 and the cover 1. The air between the seedling-raising tray 7 and the cover 1 flows out through the gap between the seedling-raising tray 7 and the cover 1, and the air in the group of divided spaces at a lower position flows out of the housing 4 through the air purification component.
[0058] This corn tissue culture device realizes the exchange of air inside and outside the housing 4, reduces the harmful gases inside the housing 4, and ensures the normal respiration of the plant seedlings in tissue culture.
[0059] Further, the second air filtration device 3 is a common air filtration device in the prior art. The second air filtration device 3 includes a tube body and a 0.22-micron or 0.45-micron filtration membrane inside the tube body.
[0060] Example 4:
[0061] As Figure 6 shown, this example discloses a corn tissue culture device, whose structure is substantially the same as that of Example 3. The difference is that at least one second air filtration device 3 is installed with a shielding cloth for adjusting the air flow resistance inside the second air filtration device 3.
[0062] The working process and principle of this example are as follows:
[0063] The operator adjusts the air flow resistance inside the second air filtration device 3 by adding or removing the shielding cloth on the second air filtration device 3, so as to adjust the moving speed of the culture solution along the length direction of the housing 4, thereby adjusting the contact time between the explant or the root of the plant seedling and the culture solution, ensuring that the explant or the root of the plant seedling is in full contact with the culture solution, and improving the reliability of this corn tissue culture device.
[0064] Example 5:
[0065] As Figure 1 、 Figure 2 shown, this example discloses a corn tissue culture device, whose structure is substantially the same as that of Example 3 or Example 4. The difference is that an air exchange hole is opened on the cover body 1 of this example, and a first air filtration device 2 is installed outside the air exchange hole.
[0066] The working process and principle of this example are as follows:
[0067] During the process of the culture solution moving along the length direction of the housing 4, the volume of the lower-positioned segmentation space group becomes smaller, causing the air pressure inside the lower-positioned segmentation space group to increase. The air inside the lower-positioned segmentation space group pushes open the water absorption block 11 and enters the space between the seedling tray 7 and the cover body 1. The air between the seedling tray 7 and the cover body 1 flows outside the housing 4 through the first air filtration device 2, and the air inside the lower-positioned segmentation space group flows outside the housing 4 through the air purification component.
[0068] The first air filtration device 2 enables the gap between the seedling tray 7 and the cover body 1 to be closed, preventing the air outside the housing 4 from entering the housing 4 without filtration.
[0069] Further, the first air filtration device 2 is a common air filtration device in the prior art. The first air filtration device 2 includes a tube body and a 0.22-micron or 0.45-micron filtration membrane inside the tube body.
[0070] Example Six:
[0071] As Figure 2 、 Figure 3 、 Figure 6 shown, this example discloses a corn tissue culture device, whose structure is roughly the same as that of Example One. The difference is that two second tubes 9 are installed on the partition 10 in this example. One end of the second tube 9 is close to the bottom of the partition 10, and the other end of the second tube 9 extends with an extension tube in a direction away from the bottom of the partition 10. The extension tubes of the two second tubes 9 are located on both sides of the partition 10 to ensure the siphon effect of the second tube 9 on the culture solution.
[0072] Example Seven:
[0073] As Figure 2 shown, this example discloses a corn tissue culture device, whose structure is roughly the same as that of Example One. The difference is that a clamping groove is opened on the top side of the partition 10 in this example, and a clamping block is detachably installed in the clamping groove. Both ends of the second tube 9 penetrate through the clamping block and extend outside the clamping block, making the replacement and maintenance of the second tube 9 convenient.
[0074] Example Eight:
[0075] As Figure 1 、 Figure 2 shown, this example discloses a corn tissue culture device, whose structure is roughly the same as that of Example One. The difference is that this example further includes a base 6 located below the housing 4. The top side of the base 6 is rotatably connected to the bottom side of the connecting frame, the connecting frame is fixed to the bottom side of the housing 4, and a driving mechanism 5 for driving the housing 4 to rotate around the width direction of the housing 4 is installed on the base 6.
[0076] The driving mechanism 5 is a jack or an electric push rod.
[0077] The driving mechanism 5 used in the present invention is a common device in the prior art, and its working mode and structure are all well-known technologies, which will not be elaborated here.
[0078] The working process and principle of this example are:
[0079] The driving mechanism 5 pushes the housing 4 to rotate around the width direction of the housing 4.
[0080] Example Nine:
[0081] As Figure 2 shown, this example discloses a corn tissue culture device, whose structure is roughly the same as that of Example One. The difference is that the cover body 1 is made of transparent glass material or transparent plastic material, and the housing 4 is made of transparent glass material or transparent plastic material.
[0082] The working process and principle of this embodiment are as follows:
[0083] The operator can observe the growth of explants or plant seedlings through the cover 1, and can observe the position and volume of the culture solution through the housing 4.
[0084] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention.
Claims
1. A corn tissue culture device, characterized in that: It comprises a shell (4) and a seedling tray (7), wherein a accommodating space is provided on the top side of the shell (4), a plurality of partitions (10) are fixed on the bottom side of the accommodating space along the length direction of the shell (4), and the partitions (10) divide the lower half of the accommodating space into a plurality of divided spaces, and a second tube (9) is passed through each partition (10), and both ends of the second tube (9) are bent downwards; The seedling tray (7) is located in the accommodating space, and the seedling tray (7) comprises a plurality of cultivation shells arranged along the length direction of the shell (4) and fixedly connected together at the top side, each cultivation shell having a plurality of cultivation grooves on the top side, each cultivation groove having a plurality of liquid holes (13) on the bottom side, a cultivation shell inserted in each divided space, and an openable cover (1) installed on the top side of the shell (4); Two second tube bodies (9) are mounted on the partition (10), one end of the second tube body (9) is close to the bottom of the partition (10), and the other end of the second tube body (9) is provided with an extension tube extending in a direction away from the bottom of the partition (10), and the extension tubes of the two second tube bodies (9) are located on both sides of the partition (10); Wherein, a culture solution is added into at least one divided space, an explant is placed into the culture tank, and then the cover body (1) completely covers the accommodating space, and one end of the shell (4) in the length direction is moved upward so that the angle between the shell (4) and the horizontal plane reaches a set angle, and at this time, the liquid level of the culture solution in the divided space is higher than the second tube body (9) with a lower height in the divided space; The shell (4) periodically reciprocates at a set angle around the width direction of the shell (4), so that the culture liquid reciprocates along the length direction of the shell (4).
2. The corn tissue culture device according to claim 1, characterized in that: A water absorbing block (11) is placed at the bottom of each cultivation tank to cover the liquid passage hole (13).
3. The corn tissue culture device according to claim 2, characterized in that: The edge of the seedling tray (7) and the inner wall of the accommodating space are slidably sealed and fitted, the seedling tray (7) makes each divided space closed, and exhaust holes (12) are provided at both ends of the length direction of the seedling tray (7), and air purification components are installed on the two exhaust holes (12); The air purification component comprises a first tube body (8), a mounting hole is provided on the shell (4), a second air filter device (3) is mounted on the outer side of the mounting hole, the inner side of the mounting hole is connected to one end of the first tube body (8), and the other end of the first tube body (8) is connected to the top side of the exhaust hole (12).
4. The corn tissue culture device according to claim 3, characterized in that: At least one second air filter device (3) is provided with a shielding cloth for adjusting the air flow resistance in the second air filter device (3).
5. The corn tissue culture device according to claim 3, characterized in that: The cover body (1) is provided with a ventilation hole, and a first air filter device (2) is installed outside the ventilation hole.
6. The corn tissue culture device according to claim 1, characterized in that: A card slot is provided on the top side of the partition (10), a card block is detachably mounted in the card slot, and both ends of the second tube body (9) penetrate the card block and extend outside the card block.
7. The corn tissue culture device according to claim 1, characterized in that: It also includes a base (6) located below the shell (4), the top side of the base (6) being rotatably connected to the bottom side of the connecting frame, the connecting frame being fixed to the bottom side of the shell (4), and a driving mechanism (5) for driving the shell (4) to rotate around the width direction of the shell (4) being mounted on the base (6).
8. The corn tissue culture device according to claim 1, characterized in that: The cover (1) is made of transparent glass or transparent plastic, and the shell (4) is made of transparent glass or transparent plastic.
9. The corn tissue culture device according to claim 1, characterized in that: The set angle between the housing (4) and the horizontal plane is 5°-40°.
Citation Information
Patent Citations
Plant seedling stage water culture device
CN117322316A
Plant cultivating device
CN1286018A