Feed for promoting intestinal health of piglets and preparation method thereof

Abstract

The invention relates to the field of feeds, and particularly discloses a feed for promoting intestinal health of piglets and a preparation method of the feed. The feed is prepared from the following raw material components in parts by weight: 100 parts of basic feed, 0.1 to 0.3 part of a micro-ecological microbial agent, 0.5 to 0.6 part of pleurotus ostreatus fermented Chinese herbal medicine powder, 0.2 to 0.4 part of glucose oxidase, 0.025 to 0.05 part of eutectic plant essential oil and 0.05 to 0.08 part of organic acid. The feed can effectively improve the intestinal health of piglets, enhance the balance of intestinal flora of the piglets, improve the immunity and disease resistance of the piglets, reduce the occurrence rate of diarrhea of the piglets and improve the growth performance of the piglets.

Description

Technical Field

[0001] The present application relates to the field of feed, and more specifically, to a feed for promoting the intestinal health of piglets and a preparation method thereof. Background Art

[0002] With the rapid development of modernization and intensification of large-scale pig farms and the acceleration of the market pig marketing cycle, piglets have also changed from natural weaning to early weaning of 3-4 cycles. Early weaning can improve the reproductive capacity of sows and reduce the spread of diseases, thereby improving the economic benefits of pig farming. However, due to the influence of multiple stressors such as physiology, nutrition and environment, early weaned piglets are prone to symptoms such as decreased feed intake, slow growth and development, diarrhea, disease and even death. These adverse symptoms are related to inflammatory response, oxidative stress and intestinal development. At the same time, nursing piglets is an important stage in the pig breeding process. Piglets are also prone to symptoms such as diarrhea during the nursing process, which affects the growth and development of pigs and the economic benefits of the company.

[0003] In the actual production process, antibiotics are usually added to the feed to reduce the damage caused by many stress factors to weaned piglets, reduce the risk of diarrhea in piglets, improve the feed conversion rate of piglets, and promote the growth and development of piglets. However, long-term use of antibiotics will remain in the animal body and animal food, threatening food safety, and also causing problems such as drug resistance. my country has banned the addition of antibiotics to feed since July 1, 2020. Therefore, finding and developing alternatives to antibiotic additives and providing a feed that promotes the intestinal health of piglets is of great significance to the sustainable development of my country's animal husbandry. Summary of the invention

[0004] In order to solve the above technical problems, the present application provides a feed that promotes the intestinal health of piglets and a preparation method thereof.

[0005] In the first aspect, the present application provides a feed that promotes intestinal health of piglets, which adopts the following technical solution: A feed for promoting intestinal health of piglets. The raw materials used include the following components in parts by weight: 100 parts of basic feed, 0.1-0.3 parts of microecological bacterial agents, 0.5-0.6 parts of fermented Chinese herbal medicine powder of Pleurotus ostreatus, 0.2-0.4 parts of glucose oxidase, 0.025-0.05 parts of eutectic plant essential oils and 0.05-0.08 parts of organic acids.

[0006] By adopting the above technical scheme, the feed gives full play to the synergistic effect between the various components, significantly improves the intestinal health of piglets, reduces the risk of diarrhea in piglets, improves the feed conversion rate of piglets, and promotes the growth and development of piglets. Specifically, the basic feed provides the nutritional matrix required for the growth of piglets, ensuring that basic nutritional needs are met; the combined use of microecological agents and fermented Chinese herbal powder of Pleurotus ostreatus can give full play to the synergistic effect between each other, regulate the balance of intestinal microorganisms in piglets, enhance immunity, reduce the incidence of diarrhea, and improve the appetite of piglets, accelerate the growth and development of piglets, and improve the growth performance of piglets; the addition of glucose oxidase can effectively decompose glucose in the intestine, generate hydrogen peroxide and gluconic acid, inhibit the growth of harmful bacteria, and work together with components such as microecological agents to maintain the balance of intestinal microecology in piglets, reduce their matrix inflammatory response, promote fat metabolism, and change the composition and community structure of dominant colon bacteria, significantly improving the growth performance of weaned piglets; the mixed use of eutectic plant essential oils and organic acids can not only provide low pH conditions, but also significantly increase the ability to inhibit the growth of potential pathogens, and also enhance the antibacterial effect of eutectic plant essential oils, and further synergize with components such as microecological agents to improve intestinal damage and inflammatory response in piglets, reduce the incidence of diarrhea, and improve the growth performance of piglets.

[0007] Among them, the Pleurotus ostreatus fermented Chinese herbal powder of the present application is a Chinese herbal powder fermented by Pleurotus ostreatus. After the Chinese herbal powder is fermented by Pleurotus ostreatus, the total polysaccharide content and the total alkaloid content are increased, the total saponin content is reduced, the antibacterial activity is increased, and the saponins in the Chinese herbal powder are bioconverted into active substances aglycones after fermentation, which can directly exert the efficacy. Compared with unfermented Chinese herbal powder, the Pleurotus ostreatus fermented Chinese herbal powder can give full play to the synergistic effect between Pleurotus ostreatus fermentation and Chinese herbal powder, further promote the intestinal health of piglets, reduce the incidence of diarrhea in piglets, and improve the growth performance of piglets.

[0008] The eutectic plant essential oil of the present application is an essential oil eutectic with high biological activity and stability synthesized by eutectic technology from plant essential oil. It can utilize the interaction between the active molecules of the essential oil and a suitable carrier to stabilize the liquid component of the essential oil into a solid form. Compared with ordinary plant essential oils, the eutectic plant essential oil can further improve the enhancing effect of the plant essential oil on piglets, thereby further reducing the incidence of diarrhea in piglets and improving the growth performance of piglets.

[0009] Optionally, the microecological bacterial agent includes one or more of Bacillus subtilis, Bifidobacterium, Bacillus licheniformis, Lactobacillus plantarum and Lactobacillus reuteri.

[0010] In a preferred embodiment, the microecological agent comprises Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri in a weight ratio of (0.8-1.2):1:(0.5-1.5).

[0011] By adopting the above technical solution, the combination of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri can effectively regulate the balance of intestinal microorganisms in piglets, inhibit the growth of harmful bacteria, and promote the reproduction of beneficial bacteria, thereby enhancing the immunity and digestion ability of piglets, reducing the incidence of diarrhea in piglets, and improving the growth performance of piglets. Specifically, Bacillus subtilis has strong stress resistance and colonization ability, and can quickly form a dominant flora in the intestine; Lactobacillus plantarum can produce lactic acid, reduce the pH value of the intestine, and inhibit the growth of pathogens; Lactobacillus reuteri can produce antibacterial substances, further enhancing the defense function of the intestine. The synergistic effect of the three significantly improves the effect of feed on the intestinal health of piglets.

[0012] Optionally, the herbal ingredients in the Pleurotus ostreatus fermented herbal powder include one or more of astragalus powder, hawthorn powder, licorice powder, codonopsis powder, angelica powder, cypress powder, tangerine peel powder, Shenqu powder, cypress powder, purslane powder, purslane powder, and isatis root powder.

[0013] In a preferred embodiment, the Chinese herbal ingredients include astragalus powder, licorice powder, hawthorn powder and isatis root powder in a weight ratio of (10-12): (9-11): (10-12): (8-10).

[0014] By adopting the above technical solution, the Chinese herbal medicine ingredients of astragalus powder, licorice powder, hawthorn powder and isatis root powder are used in a specific proportion, which can effectively enhance the immunity of piglets, improve digestive function, and enhance disease resistance, thereby significantly improving the growth performance and health level of piglets. Specifically, astragalus powder has an immunomodulatory effect, licorice powder helps relieve stress response, hawthorn powder promotes digestion and absorption, and isatis root powder has antibacterial and anti-inflammatory effects. These ingredients work synergistically to maintain the balance of intestinal microorganisms in piglets and reduce the incidence of diseases.

[0015] Optionally, the eutectic plant essential oil is a mixture of plant essential oil and L-proline eutectic, wherein the content of the plant essential oil in the overall mixture is 23-27wt%.

[0016] Optionally, the plant essential oil includes one or more of cinnamaldehyde, thymol, carvacrol and eugenol.

[0017] In a preferred embodiment, the plant essential oil comprises cinnamaldehyde and thymol in a weight ratio of 1:(0.8-1.2).

[0018] By adopting the above technical solution, the specific ratio of cinnamaldehyde and thymol can significantly improve the antibacterial effect of feed, enhance the intestinal health of piglets, and reduce the occurrence of intestinal diseases. Specifically, cinnamaldehyde has strong antibacterial activity, while thymol can effectively inhibit the growth of harmful microorganisms. The synergistic effect of the two further improves the overall antibacterial performance of the feed of this application.

[0019] In a preferred embodiment, the organic acid includes lactic acid, citric acid and formic acid, wherein the content of lactic acid in the organic acid is not less than 30wt%, the content of citric acid in the organic acid is not less than 15wt%, and the content of formic acid in the organic acid is not less than 5wt%.

[0020] By adopting the above technical scheme, the combined use of lactic acid, citric acid and formic acid can effectively regulate the pH value of the piglet's intestine, inhibit the growth of harmful microorganisms, and promote the reproduction of beneficial bacteria. Lactic acid content of not less than 30wt% helps to maintain the stability of the intestinal environment and enhance the digestion and absorption capacity of piglets. Citric acid content of not less than 15wt% can further enhance the intestinal antioxidant capacity and immune function. Formic acid content of not less than 5% can effectively inhibit the reproduction of pathogenic bacteria such as Escherichia coli and reduce the incidence of diarrhea.

[0021] In a preferred embodiment, the basal diet comprises ethanolic Clostridium protein and soybean meal, and the weight ratio of ethanolic Clostridium protein to soybean meal is (1-3): (2-6).

[0022] Through the above technical scheme, ethanol Clostridium protein is used to partially replace soybean meal and added to the basic feed, which can not only improve the growth performance of piglets, but also improve the antioxidant level, and can improve intestinal microorganisms and maintain intestinal health.

[0023] In a second aspect, the present application provides a method for preparing a feed for promoting intestinal health of piglets, which adopts the following technical solution: A method for preparing a feed for promoting intestinal health of piglets comprises the following steps: The basic feed, the microecological bacterial agent, the fermented Chinese herbal medicine powder of Pleurotus ostreatus, the glucose oxidase, the eutectic plant essential oil and the organic acid are mixed and stirred uniformly to obtain the feed.

[0024] In summary, this application has the following beneficial technical effects: The feed of the present application gives full play to the synergistic effect between the microecological bacterial agent, the fermented Chinese herbal powder of Pleurotus ostreatus, glucose oxidase, eutectic plant essential oil and organic acid, effectively improves the intestinal health of piglets, enhances the balance of intestinal flora, improves immunity and disease resistance, reduces the incidence of diarrhea in piglets, and improves the growth performance of piglets; Compared with the unfermented Chinese herbal powder, the Pleurotus ostreatus fermented Chinese herbal powder of the present application can give full play to the synergistic effect between the fermentation of Pleurotus ostreatus and the Chinese herbal powder, further promote the intestinal health of piglets, reduce the incidence of diarrhea in piglets, and improve the growth performance of piglets; Compared with common plant essential oils, the eutectic plant essential oil of the present application can further improve the enhancing effect of plant essential oils on piglets, thereby further reducing the incidence of diarrhea in piglets and improving the growth performance of piglets; The present application adopts ethanol Clostridium protein to partially replace soybean meal and add it into the basic feed, which can not only improve the growth performance of piglets, but also improve the antioxidant level, improve intestinal microorganisms, and maintain intestinal health. DETAILED DESCRIPTION

[0025] The present application is further described in detail below with reference to the embodiments.

[0026] Unless otherwise specified, the raw materials used in this application are all commercially available products.

[0027] The basic feed of this application is formulated into pellets according to the NRC (2012) nutritional requirement standard. Its specific composition and nutritional level are shown in Table 1. The crude protein in the nutritional level is the measured value, and the rest are calculated values: Table 1 Basic diet composition (%) and nutritional level

[0028] Among them, the crude protein content of the conventional nutritional components of ethanolic Clostridium protein is 88.86%, the total content of 18 amino acids is 75.59%, and the content of 10 essential amino acids is 37.66%; The premix provides per kg of feed: Vitamin A 11000IU, Vitamin D3 1100IU, Vitamin E 30IU, Vitamin K 2.5mg, Vitamin B 12 100μg, vitamin B1 3mg, vitamin B2 10mg, vitamin B6 8mg, niacin 30mg, D-pantothenic acid 60mg, folic acid 1.5mg, biotin 0.4mg, iron 120mg, copper 6mg, manganese 4mg, zinc 100mg, iodine 0.14mg, selenium 0.3mg.

[0029] The microecological bacterial agent of the present application includes one or more of Bacillus subtilis, Bifidobacterium, Bacillus licheniformis, Lactobacillus plantarum and Lactobacillus reuteri; In a preferred embodiment, the microecological agent includes Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri in a weight ratio of (0.8-1.2):1:(0.5-1.5); Among them, the appearance of Bacillus subtilis is white powder, with the special smell of probiotics, no corruption, no odor, the drying loss rate is ≤7%, and the number of viable bacteria is 1.24×10 11 cfu / g; Lactobacillus plantarum is a white powder with a special smell of probiotics, no corruption, no odor, and a drying loss rate of ≤7%. The number of viable bacteria is 1.05×10 11 cfu / g; Lactobacillus reuteri is white or light yellow in appearance, has a special smell of probiotics, is not corrupt, has no odor, has a drying loss rate of ≤7%, and has a viable bacterial count of 1.32×10 11 cfu / g.

[0030] The Pleurotus ostreatus fermented Chinese herbal medicine powder of the present application is fermented by Pleurotus ostreatus on Chinese herbal medicine powder composed of one or more of astragalus powder, hawthorn powder, licorice powder, codonopsis powder, angelica powder, cypress powder, tangerine peel powder, Shenqu powder, Guanzhong powder, purslane powder, and isatis root powder. The fermentation method is a conventional fermentation method in the art, that is, the Chinese herbal medicine powder is made into a solid fermentation matrix, after high-pressure sterilization, inoculated with seed liquid of Pleurotus ostreatus, the inoculation amount is 4% (V / W), and fermented at a constant temperature of 28°C for 25 days, and then dried to constant weight, crushed and passed through a 40-mesh sieve to obtain the Pleurotus ostreatus fermented Chinese herbal medicine powder; In a preferred embodiment, the Chinese herbal medicine powder comprises astragalus powder, licorice powder, hawthorn powder and isatis root powder in a weight ratio of (10-12): (9-11): (10-12): (8-10); Among them, the appearance of astragalus powder is brown-yellow, and it is obtained by water extraction and spray drying, and the active ingredient content is 10%; Licorice powder has a brown appearance and is obtained by water extraction followed by spray drying, with an active ingredient content of 30%; The appearance of hawthorn powder is brown, and it is obtained by water extraction and spray drying, and the active ingredient content is 20%; The appearance of Isatis root powder is brown. It is made by water extraction and spray drying, and the active ingredient content is 10%.

[0031] The glucose oxidase of the present application has an enzyme activity of 2180u / g and a water content of 5.5%.

[0032] The eutectic plant essential oil of the present application is a mixture of plant essential oil and L-proline prepared by conventional eutectic technology in the art, wherein the content of the plant essential oil in the overall mixture is 23-27wt%, and the plant essential oil includes one or more of cinnamaldehyde, thymol, carvacrol and eugenol; In a preferred embodiment, the content of the plant essential oil in the overall mixture is 25wt%, and the plant essential oil includes cinnamaldehyde and thymol in a weight ratio of 1:(0.8-1.2); Among them, cinnamaldehyde has the appearance of light brown liquid and the active ingredient content is 85%; Thymol appears as a clear oily liquid with an active ingredient content of 99%.

[0033] The organic acid of the present application is an acidulant for feed, and its main nutrients include lactic acid, citric acid and formic acid, wherein the content of lactic acid is ≥30%, the content of citric acid is ≥15%, and the content of formic acid is ≥5%.

[0034] Example 1.1 A method for preparing a feed for promoting intestinal health of piglets comprises the following steps: 100 kg of the basic feed prepared in Preparation Example 1, 0.1 kg of a microecological bacterial agent (the weight ratio of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri is 0.8:1:1.5), 0.6 kg of Pleurotus ostreatus fermented Chinese herbal medicine powder (the Chinese herbal medicine powder includes astragalus powder, licorice powder, hawthorn powder and isatis root powder in a weight ratio of 10:11:10:10), 0.2 kg of glucose oxidase, 0.05 kg of eutectic plant essential oil (the content of the plant essential oil in the overall mixture is 25 wt%, and the plant essential oil includes cinnamaldehyde and thymol in a weight ratio of 1:0.8) and 0.05 kg of an organic acid (lactic acid content ≥30%, citric acid content ≥15%, formic acid content ≥5%) are mixed and stirred to obtain a feed.

[0035] Example 1.2 A method for preparing a feed for promoting intestinal health of piglets comprises the following steps: 100 kg of the basic feed prepared in Preparation Example 1, 0.3 kg of a microecological bacterial agent (the weight ratio of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri is 1.2:1:0.5), 0.5 kg of Pleurotus ostreatus fermented Chinese herbal medicine powder (the Chinese herbal medicine powder includes astragalus powder, licorice powder, hawthorn powder and isatis root powder in a weight ratio of 12:9:12:8), 0.4 kg of glucose oxidase, 0.025 kg of eutectic plant essential oil (the content of the plant essential oil in the overall mixture is 25 wt%, and the plant essential oil includes cinnamaldehyde and thymol in a weight ratio of 1:1.2) and 0.08 kg of an organic acid (lactic acid content ≥30%, citric acid content ≥15%, formic acid content ≥5%) are mixed and stirred to obtain a feed.

[0036] Example 1.3 A method for preparing a feed for promoting intestinal health of piglets comprises the following steps: 100 kg of the basic feed prepared in Preparation Example 1, 0.2 kg of a microecological bacterial agent (the weight ratio of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri is 1:1:1), 0.55 kg of Pleurotus ostreatus fermented Chinese herbal medicine powder (the Chinese herbal medicine powder includes astragalus powder, licorice powder, hawthorn powder and isatis root powder in a weight ratio of 11:10:11:9), 0.3 kg of glucose oxidase, 0.0375 kg of eutectic plant essential oil (the content of the plant essential oil in the overall mixture is 25 wt%, and the plant essential oil includes cinnamaldehyde and thymol in a weight ratio of 1:1) and 0.065 kg of an organic acid (lactic acid content ≥30%, citric acid content ≥15%, formic acid content ≥5%) are mixed and stirred to obtain a feed.

[0037] Example 2.1 A method for preparing feed for promoting intestinal health of piglets, which is different from Example 1.3 in that: Bacillus subtilis in the microecological bacterial agent is removed, and the weight ratio of Lactobacillus plantarum and Lactobacillus reuteri is 1:1, and the rest is the same as Example 1.3.

[0038] Example 2.2 A method for preparing feed for promoting intestinal health of piglets, which is different from Example 1.3 in that Lactobacillus plantarum in the microecological bacterial agent is removed, and the weight ratio of Bacillus subtilis and Lactobacillus reuteri is 1:1, and the rest is the same as Example 1.3.

[0039] Example 2.3 A method for preparing feed for promoting intestinal health of piglets, which is different from Example 1.3 in that Lactobacillus reuteri in the microecological bacterial agent is removed, and the weight ratio of Bacillus subtilis and Lactobacillus plantarum is 1:1, and the rest is the same as Example 1.3.

[0040] Example 2.4 A method for preparing a feed for promoting intestinal health of piglets, which is different from Example 1.3 in that an equal amount of Bacillus subtilis in the microecological bacterial agent is replaced with Bacillus licheniformis, and the rest is the same as Example 1.3.

[0041] Example 2.5 A method for preparing a feed for promoting intestinal health of piglets, which is different from that of Example 1.3 in that an equal amount of Lactobacillus plantarum in the microecological bacterial agent is replaced with Bifidobacterium, and the rest is the same as that of Example 1.3.

[0042] Example 2.6 A method for preparing a feed for promoting intestinal health of piglets, which is different from Example 1.3 in that an equal amount of Lactobacillus reuteri in the microecological bacterial agent is replaced with Bacillus licheniformis, and the rest is the same as Example 1.3.

[0043] Example 3.1 A method for preparing feed for promoting intestinal health of piglets, which is different from Example 1.3 in that cinnamaldehyde in eutectic plant essential oil is removed, and the rest is the same as Example 1.3.

[0044] Example 3.2 A method for preparing feed that promotes intestinal health of piglets, which is different from Example 1.3 in that thymol in eutectic plant essential oil is removed, and the rest is the same as Example 1.3.

[0045] Example 3.3 A method for preparing a feed for promoting intestinal health of piglets, which is different from that of Example 1.3 in that an equal amount of cinnamaldehyde in the eutectic plant essential oil is replaced with carvacrol, and the rest is the same as that of Example 1.3.

[0046] Example 3.4 A method for preparing a feed for promoting intestinal health of piglets, which is different from Example 1.3 in that an equal amount of cinnamaldehyde in the eugenol is replaced by eugenol, and the rest is the same as Example 1.3.

[0047] Example 3.5 A method for preparing a feed that promotes intestinal health in piglets, which is different from Example 1.3 in that an equal amount of thymol in the eutectic plant essential oil is replaced with carvacrol, and the rest is the same as Example 1.3.

[0048] Example 3.6 A method for preparing a feed that promotes intestinal health in piglets, which is different from Example 1.3 in that an equal amount of thymol in the eutectic plant essential oil is replaced with eugenol, and the rest is the same as Example 1.3.

[0049] Example 4.1 A method for preparing a feed that promotes intestinal health in piglets, which is different from Example 1.3 in that the basic feed prepared in Preparation Example 1 is replaced by the basic feed prepared in Preparation Example 2, and the rest is the same as Example 1.3.

[0050] Example 4.2 A method for preparing a feed that promotes intestinal health in piglets, which is different from Example 1.3 in that the basic feed prepared in Preparation Example 1 is replaced by the basic feed prepared in Preparation Example 3, and the rest is the same as Example 1.3.

[0051] Example 4.3 A method for preparing a feed that promotes intestinal health in piglets, which is different from Example 1.3 in that the basic feed prepared in Preparation Example 1 is replaced by the basic feed prepared in Preparation Example 4, and the rest is the same as Example 1.3.

[0052] Example 4.4 A method for preparing a feed that promotes intestinal health in piglets, which is different from Example 1.3 in that the basic feed prepared in Preparation Example 1 is replaced by the basic feed prepared in Preparation Example 5, and the rest is the same as Example 1.3.

[0053] Comparative Example 1.1 The difference from Example 1.3 is that no microecological agent is added, the amount of the fermented Chinese herbal medicine powder of Pleurotus ostreatus is changed to 0.75 kg, and the rest is the same as Example 1.3.

[0054] Comparative Example 1.2 The difference from Example 1.3 is that: no Pleurotus ostreatus fermented Chinese herbal medicine powder is added, the amount of microecological agent is changed to 0.75 kg, and the rest is the same as Example 1.3.

[0055] Comparative Example 1.3 The difference from Example 1.3 is that glucose oxidase is not added, the dosage of the microecological agent is changed to 0.5 kg, and the rest is the same as Example 1.3.

[0056] Comparative Example 1.4 The difference from Example 1.3 is that no eutectic plant essential oil is added, the amount of organic acid is changed to 0.1025 kg, and the rest is the same as Example 1.3.

[0057] Comparative Example 1.5 The difference from Example 1.3 is that no organic acid is added, the amount of eutectic plant essential oil is changed to 0.1025 kg, and the rest is the same as Example 1.3.

[0058] Comparative Example 2.1 The difference from Example 1.3 is that the herbal powder fermented by Pleurotus ostreatus is replaced by herbal powder that has not been fermented by Pleurotus ostreatus, that is, the herbal powder (the weight ratio of astragalus powder, licorice powder, hawthorn powder and isatis root powder is 11:10:11:9) is made into a solid fermentation matrix, which is sterilized by high pressure and inoculated with potato glucose liquid culture medium at an inoculation amount of 4% (V / W). It is fermented at a constant temperature of 28°C for 25 days, and then dried to constant weight, crushed through a 40-mesh sieve to obtain the herbal powder fermented by Pleurotus ostreatus, and the rest is the same as Example 1.3.

[0059] Comparative Example 3.1 The difference from Example 1.3 is that the eutectic plant essential oil is replaced with common essential oil, that is, 0.01875 kg of cinnamaldehyde and 0.01875 kg of thymol are directly mixed and added to the feed, and the rest is the same as Example 1.3.

[0060] Performance Testing 270 healthy Sanyuan (Du × Chang × Da) weaned piglets of 28 days old with good health and good physical condition, average weight of (14 ± 0.4) kg were selected and randomly divided into 27 groups, each group of 10 piglets (half male and half female), and fed with basal diet (i.e., the basal diet prepared in Preparation Example 1 as the control group), the feeds of Examples 1.1-4.4 and Comparative Examples 1.1-3.1, respectively, and the test period was 35 days. Before the formal test, all piglets were pre-tested with the basal diet prepared in Preparation Example 1 for 7 days. During the test, the piglets were raised in a sterilized fully enclosed temperature-controlled room. The piglets were fed 4 times a day (08:00, 14:00, 18:00 and 20:00), and they were free to eat and drink water. The feed trough was checked every day to ensure that there was always feed in the feed trough to ensure free feeding and minimize feed waste. The temperature was controlled at (28 ± 1) ° C in the first week of the test, and then gradually decreased to 25 ° C until the end of the test. No vaccinations or antibiotics were given to the pigs during the entire trial period.

[0061] (1) Growth performance measurement: All pigs were weighed on an empty stomach before 08:00 on the first and 35th days of the experiment, and the starting and ending weights were recorded for calculating the average daily weight gain (ADR) = (average ending weight of each group - average starting weight of each group) / experimental time; (2) Diarrhea rate: During the trial, the health of all pigs was observed every day, the total number of diarrhea cases in each group of pigs was counted (repeated calculation for the same pig), and the diarrhea rate of each group of pigs was calculated. Diarrhea rate (%) = total number of diarrhea piglets in each group during the trial period / (total number of piglets in each group during the trial period × number of test days) × 100%; (3) Disease status: The health status of all pigs was observed every day during the experiment, and the number of sick pigs in each group during the experiment was recorded; The above test results are shown in Table 2.

[0062] Table 2 Test results

[0063] Data Analysis: As can be seen from Table 2, the daily weight gain of Examples 1.1-1.3 of the present application is 420.08-423.51 g, which is much higher than 341.14 g of the control group, the diarrhea rate is 3.05-3.15%, which is much lower than 9.64% of the control group, and the number of sick pigs is 1, which is much lower than 5 in the control group. The test results show that feeding piglets with the feed prepared by Examples 1.1-1.3 of the present application can effectively improve the intestinal health of piglets, improve the immunity and disease resistance of piglets, reduce the incidence of diarrhea in piglets, and improve the growth performance of piglets.

[0064] The difference between Example 2.1-2.6 and Example 1.3 is that the three strains of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri are not mixed and used. It can be seen from Table 2 that the daily weight gain of Example 2.1-2.6 is less than that of Example 1.3, and the diarrhea rate is higher than that of Example 1.3. The test results show that compared with the combination of other microecological bacterial agents, the use of Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri in a mixed combination can give full play to the synergistic effect between each other, reduce the incidence of diarrhea in piglets, and improve the growth performance of piglets.

[0065] The difference between Examples 3.1-3.6 and Example 1.3 is that thymol and cinnamaldehyde are not mixed and used. It can be seen from Table 2 that the daily weight gain of Examples 3.1-3.6 is less than that of Example 1.3, and the diarrhea rate is higher than that of Example 1.3. The test results show that compared with other combinations of plant essential oils, the mixed use of thymol and cinnamaldehyde can not only give full play to the synergistic effect between the two, but also can play a good synergistic role with other components such as microecological agents in feed, thereby reducing the incidence of diarrhea in piglets and improving the growth performance of piglets.

[0066] The difference between Example 4.1-4.4 and Example 1.3 is that ethanolic clostridium protein is further added to the basic feed to replace soybean meal. It can be seen from Table 2 that the daily weight gain of Example 4.1-4.4 is greater than that of Example 1.3, and the diarrhea rate is lower than that of Example 1.3. The test results show that the addition of ethanolic clostridium protein can not only improve the growth performance of piglets, but also improve the antioxidant level, and can improve intestinal microorganisms and maintain intestinal health. By comparing the data of Example 1.3 and Example 4.1-4.4, it can be found that with the increase in the amount of ethanolic clostridium protein added, the daily weight gain of piglets shows a trend of first increasing and then decreasing, and the diarrhea rate shows a trend of first decreasing and then increasing. It can be seen that if the amount of ethanolic clostridium protein added is too much, it will increase the diarrhea rate of piglets and reduce the growth performance of piglets.

[0067] The difference between Comparative Examples 1.1-1.5 and Example 1.3 is that any one of the microecological agents, fermented Chinese herbal powder of Pleurotus ostreatus, glucose oxidase, eutectic plant essential oil and organic acid is lacking. As can be seen from Table 2, the daily weight gain of Comparative Examples 1.1-1.5 is less than that of Example 1.3, the diarrhea rate is higher than that of Example 1.3, and the number of sick heads during the test is higher than that of Example 1.3. The test results show that the lack of any one of the microecological agents, fermented Chinese herbal powder of Pleurotus ostreatus, glucose oxidase, eutectic plant essential oil and organic acid cannot exert a synergistic effect with each other, thereby reducing the improvement effect of feed on the intestinal tract of piglets, increasing the incidence of diarrhea in piglets, and reducing the growth performance of piglets.

[0068] The difference between Comparative Example 2.1 and Example 1.3 is that the herbal powder fermented by Pleurotus ostreatus is replaced with herbal powder that has not been fermented by Pleurotus ostreatus. It can be seen from Table 2 that the daily weight gain of Comparative Example 2.1 is less than that of Example 1.3, the diarrhea rate is higher than that of Example 1.3, and the number of sick heads during the test is higher than that of Example 1.3. The test results show that fermenting the herbal powder with Pleurotus ostreatus can further exert the synergistic effect between the two and promote the efficacy of the herbal powder, thereby reducing the incidence of diarrhea in piglets and improving the growth performance of piglets.

[0069] The difference between Comparative Example 3.1 and Example 1.3 is that the eutectic plant essential oil is replaced with ordinary plant essential oil. It can be seen from Table 2 that the daily weight gain of Comparative Example 3.1 is less than that of Example 1.3, the diarrhea rate is higher than that of Example 1.3, and the number of sick heads during the test is higher than that of Example 1.3. The test results show that by combining the plant essential oil with the carrier using eutectic technology, the stability of the plant essential oil can be improved, the effect of the plant essential oil can be promoted, thereby reducing the incidence of diarrhea in piglets and improving the growth performance of piglets.

[0070] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A feed for promoting intestinal health of piglets, characterized in that: The raw materials used include the following components in parts by weight: 100 parts of basic feed, 0.1-0.3 parts of microecological bacteria, 0.5-0.6 parts of fermented Chinese herbal medicine powder of Pleurotus ostreatus, 0.2-0.4 parts of glucose oxidase, 0.025-0.05 parts of eutectic plant essential oil and 0.05-0.08 parts of organic acid.

2. A feed for promoting intestinal health of piglets according to claim 1, characterized in that: The microecological bacterial agent includes one or more of Bacillus subtilis, Bifidobacterium, Bacillus licheniformis, Lactobacillus plantarum and Lactobacillus reuteri.

3. A feed for promoting intestinal health of piglets according to claim 2, characterized in that: The microecological bacterial agent comprises Bacillus subtilis, Lactobacillus plantarum and Lactobacillus reuteri in a weight ratio of (0.8-1.2):1:(0.5-1.5).

4. A feed for promoting intestinal health of piglets according to claim 1, characterized in that: The Chinese herbal ingredients in the Pleurotus ostreatus fermented Chinese herbal powder include astragalus powder, licorice powder, hawthorn powder and isatis root powder in a weight ratio of (10-12): (9-11): (10-12): (8-10).

5. A feed for promoting intestinal health of piglets according to claim 1, characterized in that: The eutectic plant essential oil is a mixture of plant essential oil and L-proline eutectic, wherein the content of the plant essential oil in the overall mixture is 23-27wt%.

6. A feed for promoting intestinal health of piglets according to claim 5, characterized in that: The plant essential oil comprises one or more of cinnamaldehyde, thymol, carvacrol and eugenol.

7. A feed for promoting intestinal health of piglets according to claim 6, characterized in that: The plant essential oil comprises cinnamaldehyde and thymol in a weight ratio of 1:(0.8-1.2).

8. The feed for promoting intestinal health of piglets according to claim 1, characterized in that: The organic acid includes lactic acid, citric acid and formic acid, wherein the content of lactic acid in the organic acid is not less than 30wt%, the content of citric acid in the organic acid is not less than 15wt%, and the content of formic acid in the organic acid is not less than 5wt%.

9. The feed for promoting intestinal health of piglets according to claim 1, characterized in that: The basic feed includes ethanolic clostridium protein and soybean meal, and the weight ratio of ethanolic clostridium protein to soybean meal is (1-3): (2-6).

10. A method for preparing a feed for promoting intestinal health of piglets according to any one of claims 1 to 9, characterized in that: The following steps are involved: The basic feed, the microecological bacterial agent, the fermented Chinese herbal medicine powder of Pleurotus ostreatus, the glucose oxidase, the eutectic plant essential oil and the organic acid are mixed and stirred uniformly to obtain the feed.

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