Mild soothing and repairing plant composition and preparation method thereof

Abstract

The invention provides a mild soothing and repairing plant composition and a preparation method thereof, and belongs to the technical field of cosmetics. The plant composition is prepared from a centella asiatica extract, a chamomilla recutita flower extract and a pomelo extract in a mass ratio of (0.7-1.5): (0.9-1.5): (2-5). The plant extracts in the plant composition provided by the invention are tightly matched and synergistically improve the soothing and repairing effects, inflammation anaphylaxis caused by external stimulation can be effectively reduced, the damaged skin barrier is repaired, and the plant composition is mild and non-irritant, has an excellent soothing effect on sensitive skin and improves comfort.

Description

Technical Field

[0001] The invention belongs to the technical field of daily cosmetics, and particularly relates to a mild, soothing and repairing plant composition and a preparation method thereof. Background Art

[0002] Sensitive skin is a sub-healthy skin condition that is highly sensitive to many external factors, causing subjective abnormal sensations, especially on the face. It is highly reactive and low-tolerant, and clinical symptoms mainly include paroxysmal or periodic burning, flushing, tingling, itching, and tightness, or accompanied by objective signs such as erythema, scaling, and capillary dilation. The incidence of sensitive skin is high in countries around the world, and the incidence in women is generally higher than that in men. In addition, with the continuous aggravation of environmental pollution and the increase of mental stress, the incidence of sensitive skin is increasing year by year.

[0003] Sensitive skin is a subjective sensory symptom induced by a variety of factors. In the medical field, it is usually regarded as a skin condition rather than a skin disease. Its cause is not yet fully understood, but its occurrence is mainly the result of the combined action of endogenous and exogenous factors. Endogenous factors include genetics, age, gender, hormone levels, and mental factors. Studies in recent years have shown that sensitive skin is related to genetics, and mental stress can reflexively promote the release of neurotensin, thereby causing sensitive skin. Exogenous factors can cause or aggravate sensitive skin, such as seasonal changes, temperature changes, and sun exposure among physical factors; cosmetics, disinfection products, air pollutants among chemical factors; and topical irritants and long-term and large-scale use of glucocorticoids among medical factors.

[0004] At present, there is a lack of consensus strategies for the treatment of sensitive skin, and the medical community is still in the exploratory research stage for the understanding and definition of this multifactorial syndrome. In terms of treatment, no targeted diagnosis and treatment plan has yet been formed. Treatment is mainly based on the cause and pathogenesis of the disease, with the principles of psychological counseling for patients, skin care health education, repairing damaged skin barriers, reducing inflammatory responses, and reducing neurovascular hyperreactivity, so as to improve skin tolerance and avoid various triggering factors as much as possible. Although cold spray and laser treatment in physical therapy can restore the skin barrier, due to the complex inducing factors of sensitive skin, large individual differences in clinical symptoms, and easy recurrence of the disease, the barrier recovery time of physical therapy is long and the effect is limited. In terms of drug treatment, hydroxychloroquine, antihistamines, nonsteroidal anti-inflammatory drugs or glucocorticoids are commonly used in clinical practice, but these drugs will have certain side effects if used for a long time, and improper use will aggravate the condition. At present, there is a lack of safe, non-irritating, effective and suitable long-term drugs to replace chemical drugs. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a mild soothing and repairing plant composition and a preparation method, so as to provide an effective, mild, safe and non-irritating treatment plan for susceptible people, so as to effectively solve sensitive skin problems.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a mild soothing and repairing plant composition, which comprises the following ingredients: Centella asiatica extract, chamomile extract and pomelo extract; wherein the mass ratio of the Centella asiatica extract, the chamomile extract and the pomelo extract is (0.7-1.5): (0.9-1.5): (2-5).

[0008] Preferably, the Centella asiatica extract contains asiatica glycoside and madecassoside in a total amount of ≥3.0%; the chamomile flower extract contains apigenin and cosmosin in a total amount of ≥2.0%; and the pomelo extract contains naringin and neohesperidin in a total amount of ≥5.0%.

[0009] Preferably, the pomelo extract is any one of grapefruit fruit extract and pomelo fruit extract or a combination of both.

[0010] The present invention also provides a method for preparing a mild soothing and repairing plant composition, comprising the following steps:

[0011] 1) mixing Centella asiatica extract, chamomile flower extract and grapefruit extract according to proportion;

[0012] 2) After mixing and stirring evenly, a certain amount of water is added to prepare an extract mixed solution;

[0013] 3) Centrifuge the prepared mixed solution, take the supernatant and filter it to obtain the product.

[0014] As an embodiment, the mass concentration of the mixed solution of extracts in step 2) is 1-3%.

[0015] As an embodiment, the centrifugation condition in step 3) is 3500-5000 rpm, 25-35 min.

[0016] The present invention also provides the use of the mild soothing and repairing plant composition in the preparation of anti-skin allergy products.

[0017] The present invention also provides the use of the mild soothing and repairing plant composition in the preparation of skin anti-inflammatory products.

[0018] The present invention also provides the use of the mild soothing and repairing plant composition in the preparation of a skin barrier repair product.

[0019] Preferably, the product includes lotions, ointments, creams, emulsions and serums for skin.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention provides a mild soothing and repairing plant composition and a preparation method. The plant composition obtained by the method is entirely made of natural plant extracts, and the components are closely matched and synergistic, showing excellent efficacy in anti-inflammatory, anti-allergic and skin barrier repair. Compared with traditional chemical and hormone drugs, the composition has no irritation to human skin and its effect is far superior to the former. It can be used as a key soothing ingredient and is widely used in the formulation of daily chemical products, which can perfectly meet the soothing needs of sensitive skin.

[0022] The preparation process of the plant composition provided by the present invention does not use solvents such as ethanol that are difficult to remove and cause skin irritation, and its preparation process is simpler, which greatly reduces the production cost. It ensures that stable quality and excellent efficacy can still be maintained in the case of large-scale production. This makes the plant composition have extremely high application value and broad market prospects in the field of daily chemical products, can meet the needs of production enterprises of different scales, and bring more high-quality soothing product options to people with sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The inhibition rate of β-Hex release by the embodiments, comparative examples and positive drugs.

[0024] Figure 2 Effects of Examples, Comparative Examples and Positive Drugs on TNF-α Expression.

[0025] Figure 3 Effects of Examples, Comparative Examples and Positive Drugs on IL-1β Expression.

[0026] Figure 4 Effects of the embodiments and comparative examples on the change of the length of the fish embryo tail fin. DETAILED DESCRIPTION

[0027] The present invention provides a mild soothing and repairing botanical composition, which comprises the following ingredients: Centella asiatica extract, chamomile extract and pomelo extract. The mass ratio of the three extracts of Centella asiatica extract, chamomile extract and pomelo extract is (0.7-1.5): (0.9-1.5): (2-5).

[0028] In the present invention, the Centella asiatica extract is derived from Centella asiatica (L.) Urban., also known as horse hoof grass, which has the effects of clearing away heat and dampness, promoting blood circulation and stopping bleeding, detoxifying and reducing swelling. It is widely distributed in my country and has abundant resources. At present, domestic and foreign studies have found that Centella asiatica contains a variety of chemical components such as triterpenoid saponins, flavonoids, volatile oils, etc., and has good efficacy on skin diseases. In the present invention, the function of the Centella asiatica extract is anti-inflammatory and promoting the synthesis of collagen. In the present invention, the selected Centella asiatica extract contains asiaticoside and madecassoside, and the total amount of asiaticoside and madecassoside is ≥3.0%.

[0029] In the present invention, the chamomile flower extract is derived from Matricaria recutita L. chamomile, also known as chamomile, an annual herbaceous plant of the genus Matricaria in the Asteraceae family, which has the effects of clearing away heat and detoxifying, relieving cough and asthma, and removing rheumatism. Modern pharmacological studies have shown that the chemical components of chamomile mainly include flavonoids, phenolic acids, volatile oils, coumarins and other components, which have sedative, anti-inflammatory, antibacterial, antipruritic and other effects. In the present invention, the chamomile flower extract has anti-inflammatory, analgesic, antibacterial, anti-allergic and repair-promoting effects. In the present invention, the chamomile flower extract contains apigenin and cosmos glycosides, and the total amount of apigenin and cosmos glycosides is ≥2.0%.

[0030] In the present invention, the pomelo extract is any one or a combination of two of grapefruit fruit extract and pomelo fruit extract. Grapefruit (Citrus paradise Macfadyen.) and pomelo (Citrus maxima (Burm.) Merr.) are both woody plants of the genus Citrus in the Rutaceae family. Modern medicine shows that grapefruit has antioxidant, antibacterial, soothing and other effects. Pomelo is the mature fruit of pomelo, which contains neohesperidin, naringin and limonene. In the present invention, the grapefruit fruit extract or pomelo fruit extract contains naringin and neohesperidin, and the total amount of naringin and neohesperidin is ≥5.0%.

[0031] In the present invention, Centella asiatica extract, chamomile flower extract and pomelo extract are compounded as components, and the components have a synergistic effect. The three are added and used in a raw material mass ratio of (0.7-1.5): (0.9-1.5): (2-5). Preferably, the mass ratio of the Centella asiatica extract, chamomile flower extract and pomelo extract is (0.9-1.3): (1-1.5): (2.5-4). More preferably, the mass ratio of the Centella asiatica extract, chamomile flower extract and pomelo extract is 1:1:3.

[0032] The present invention also provides a method for preparing a mild soothing and repairing plant composition, the preparation method comprising the following steps: 1) mixing Centella asiatica extract, chamomile extract, and pomelo extract in proportion; 2) adding a certain amount of water to mix and stir evenly to prepare an extract mixed solution; 3) centrifuging the obtained mixed solution, taking the supernatant and filtering it to obtain. The mass concentration of the extract mixed solution is 0.1-10.0%. Further preferably, the mass concentration of the extract mixed solution is 0.1-5%. Further, the final mass concentration is 1-3%. The obtained extract mixed solution is further centrifuged, and the centrifugal condition is centrifuged at 2500-5000rpm for 20-40min. Preferably, the centrifugal condition is centrifuged at 3500-5000rpm for 25-35min. Further preferably, the centrifugal condition is centrifuged at 5000rpm for 30min. The preparation process of the plant composition provided by the present invention does not use solvents such as ethanol that are not easy to remove and cause skin irritation, and the configuration process is simpler, the cost is lower, and it is easy to scale up production.

[0033] In the present invention, the sources of the Centella asiatica extract, chamomile flower extract, grapefruit fruit extract and pomelo fruit extract are not particularly limited and can be obtained by conventional technical means in the art or purchased from commercial products in the art.

[0034] In the present invention, RBL-2H3 cells were selected as experimental subjects to verify the efficacy of the mild soothing and repairing plant composition in anti-allergy. RBL-2H3 cells were stimulated with C48 / 80 to be in an allergic state, and then different combinations of plant compositions and positive drugs were given for culture. The experimental results showed that Centella asiatica extract, chamomile extract, grapefruit fruit extract and pomelo fruit extract exhibited good anti-allergic activity. Moreover, when these extracts were compounded in a specific ratio, it was confirmed that the compounding of different extracts had a certain synergistic effect, which was better than the positive drug, and achieved significant soothing results. At the same time, RAW264.7 macrophages were used as experimental subjects, and different combinations of plant compositions were first given, followed by lipopolysaccharide (LPS) modeling. The results showed that the plant composition compounded in a certain ratio can reduce the secretion of intracellular TNF-α and IL-1β, showing a significant anti-inflammatory effect, and also reflects that the compounding of different extracts has a certain synergistic effect. Zebrafish and humans have up to 87% disease-related gene similarity. Compared with in vitro cell experiments, zebrafish are used as complete organisms for experiments, and their test results are more reliable. The present invention uses zebrafish as experimental subjects to verify the role of the mild, soothing and repairing plant composition in repairing damage. Healthy zebrafish embryos are selected, their tail fins are mechanically damaged, and then different combinations of plant compositions are given for treatment. The results show that the plant composition can effectively promote the growth of zebrafish tail fins, has a certain tissue damage repair effect, and the compound extracts in a specific proportion have a certain synergistic effect. The present invention proves through cell and animal experiments that the components of the plant extracts are closely matched and synergistic, and show significant effects in anti-inflammatory, anti-allergic and skin tissue barrier repair. In addition, by comparing with traditional chemical and hormone drugs, it is confirmed that the composition has no irritation, and its effect is far superior to chemical drugs and hormone drugs.

[0035] In the present invention, the use of the plant composition described in the above technical solution in the preparation of anti-skin allergy products, skin anti-inflammatory products and skin barrier repair products is provided. The products prepared by the plant composition of the present application include but are not limited to products in the form of water, ointment, cream, lotion, essence and the like for skin.

[0036] In the embodiments of the present invention, all data were statistically analyzed using the software SPSS 19.0, and the quantitative data were expressed in the form of mean ± standard deviation, * indicates P < 0.01, and *** indicates P < 0.05.

[0037] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0038] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0039] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0040] Example 1

[0041] The three extracts are combined in a mass ratio of Centella asiatica extract: Chamomile flower extract: grapefruit fruit extract or pomelo fruit extract = 1:1:3, and water is added after mixing to form a composition mixed solution with a mass concentration of 2.0%, and centrifuged at 5000rpm for 25min. The obtained supernatant is filtered to obtain a mild soothing and repairing plant composition provided by the present invention. Among them, the Centella asiatica extract contains asiaticoside and hydroxy-madecassoside, and the total amount is 3.0%. The Chamomile flower extract contains apigenin and cosmos glycoside, and the total amount is 2.0%. The grapefruit fruit extract or pomelo fruit extract contains naringin and neohesperidin, and the total amount is 5.0%.

[0042] Example 2

[0043] Centella asiatica extract: chamomile extract: grapefruit fruit extract or pomelo fruit extract are combined in a mass ratio of 0.9:1.5:2.5, and water is added after mixing to form a composition mixed solution with a mass concentration of 3.0%, and centrifuged at 3500rpm for 35min, and the obtained supernatant is filtered to obtain a mild soothing and repairing plant composition provided by the present invention. Among them, the Centella asiatica extract contains asiaticoside and hydroxy-madecassoside, and the total amount thereof is 3.0%. The chamomile flower extract contains apigenin and cosmos glycoside, and the total amount thereof is 2.0%. The grapefruit fruit extract or pomelo fruit extract contains naringin and neohesperidin, and the total amount thereof is 5.0%.

[0044] Example 3

[0045] Centella asiatica extract: chamomile extract: grapefruit fruit extract or pomelo fruit extract are combined in a mass ratio of 1.3:1:4, and water is added after mixing to form a composition mixed solution with a mass concentration of 1%, and centrifuged at 2500rpm for 40min, and the obtained supernatant is filtered to obtain a mild soothing and repairing plant composition provided by the present invention. Among them, the Centella asiatica extract contains asiaticoside and hydroxymadecassoside, and the total amount thereof is 3.0%. The chamomile flower extract contains apigenin and cosmos glycoside, and the total amount thereof is 2.0%. The grapefruit fruit extract or pomelo fruit extract contains naringin and neohesperidin, and the total amount thereof is 5.0%.

[0046] Comparative Example 1

[0047] The Centella asiatica extract was added to water to prepare a mixed solution with a mass concentration of 2.0%, and the remaining operation methods were the same as those in Example 1, so as to obtain a solution with a concentration of 2.0% of the Centella asiatica extract. The Centella asiatica extract contained asiaticoside and madecassoside, and the total amount thereof was 3.0%.

[0048] Comparative Example 2

[0049] The chamomile extract was added to water to prepare a mixed solution with a mass concentration of 2.0%, and the remaining operation method was the same as that of Example 1, so as to obtain a solution with a concentration of 2.0% of the chamomile extract. The chamomile extract contained apigenin and cosmosin, and the total amount thereof was 2.0%.

[0050] Comparative Example 3

[0051] Grapefruit fruit extract or pomelo fruit extract is added to water to prepare a composition mixed solution with a mass concentration of 2.0%, and the remaining operation methods are the same as those in Example 1, so as to obtain a solution with a concentration of 2.0% of grapefruit fruit extract or pomelo fruit extract. Among them, the grapefruit fruit extract or pomelo fruit extract contains naringin and neohesperidin, and the total amount thereof is 5.0%.

[0052] Comparative Example 4

[0053] Centella asiatica extract and chamomile extract are combined in a mass ratio of 1:1, and water is added to prepare a mixed solution with a mass concentration of 2.0%. The remaining operation methods are the same as those in Example 1, and a solution with a mass concentration of 2.0% is obtained after the Centella asiatica extract and chamomile extract are combined. Among them, the Centella asiatica extract contains asiaticoside and madecassoside, and the total amount thereof is 3.0%. The chamomile extract contains apigenin and cosmosin, and the total amount thereof is 2.0%.

[0054] Comparative Example 5

[0055] Centella asiatica extract: grapefruit fruit extract or pomelo fruit extract = 1:3 mass ratio, add water to prepare a composition mixed solution with a mass concentration of 2.0%, and the rest of the operation method is the same as that of Example 1, so as to obtain a solution with a mass concentration of 2.0% after the Centella asiatica extract, grapefruit fruit extract or pomelo fruit extract are combined. Among them, the Centella asiatica extract contains asiaticoside and madecassoside, and the total amount thereof is 3.0%. The grapefruit fruit extract or pomelo fruit extract contains naringin and neohesperidin, and the total amount thereof is 5.0%.

[0056] Comparative Example 6

[0057] The matricaria extract and grapefruit extract or pomelo extract are combined in a mass ratio of 1:3, and water is added to prepare a mixed solution of a composition with a mass concentration of 2.0%. The remaining operation methods are the same as those in Example 1, so as to obtain a solution with a mass concentration of 2.0% after the matricaria extract, grapefruit extract or pomelo extract are combined. Among them, the matricaria extract contains apigenin and cosmosin, and the total amount thereof is 2.0%. The grapefruit extract or pomelo extract contains naringin and neohesperidin, and the total amount thereof is 5.0%.

[0058] Comparative Example 7

[0059] The three extracts are combined in a mass ratio of Centella asiatica extract: Chamomile flower extract: grapefruit fruit extract or pomelo fruit extract = 1:1:1, and water is added after mixing to form a composition mixed solution with a mass concentration of 2.0%. The remaining operation methods are the same as those in Example 1, and a solution with a mass concentration of 2.0% of the three extracts is obtained. Among them, the Centella asiatica extract contains asiaticoside and hydroxy-madecassoside, and the total amount thereof is 3.0%. The Chamomile flower extract contains apigenin and cosmosin, and the total amount thereof is 2.0%. The grapefruit fruit extract or pomelo fruit extract contains naringin and neohesperidin, and the total amount thereof is 5.0%.

[0060] Example 4 Anti-allergic activity test

[0061] 1. Experimental Methods

[0062] 1.1 RBL-2H3 cell culture

[0063] RBL-2H3 cells were placed in MEM / EBSS medium and cultured at 37°C and 5% CO2, and passaged every 2 days.

[0064] 1.2 Detection of β-Hex degranulation in activated RBL-2H3 cells

[0065] Prepare the cells in the logarithmic growth phase into a cell suspension and adjust the concentration to 2.0×10 5cells / mL, inoculated into a 24-well plate, 1000 μL per well, cultured for 12 hours, discarded the supernatant, and washed twice with PBS buffer. 1000 μL of the samples of Example 1 and Comparative Examples 1 to 7 were added respectively, with 5 μM azelastine as a positive control, and 3 replicates were set at the same time, which were defined as the drug-treated group, and the model group and blank group were set at the same time. After incubation for 2 hours, the supernatant was discarded, and the PBS buffer was washed twice. 1000 μL of C48 / 80 medium solution with a concentration of 80 μL / mL was added to each well of the drug-treated group and the model group, and the blank group was replaced with an equal amount of medium, placed in an incubator and incubated for 40 minutes, and then ice-bathed for 10 minutes. The supernatant of each group was collected, 50 μL was taken from each well and added to a 96-well plate, 50 μL of colorimetric solution (citric acid / sodium citrate buffer solution containing 1 mmol / L p-nitrobenzene-N-acetyl-β-D-glucosaminide) was added, and after incubation at 37°C for 1 h, 200 μL of stop solution (Na2CO3-NaHCO3 buffer solution) was added to terminate the reaction, and the OD value of each well was detected by an enzyme reader at a wavelength of 450 nm.

[0066]

[0067] Among them: A 模型 is the OD value of the model group; A 空白 is the OD value of the blank group; A 给药 is the OD value of the drug-treated group.

[0068] 2. Experimental results

[0069] β-Hexosaminidase (β-Hex) is a marker of mast cell degranulation, and its release degree is positively correlated with histamine, the main mediator of allergic reactions. The β-Hex release rate of the model group increased significantly after C48 / 80 stimulation. Experimental detection found that compared with the model group, Example 1 and the positive drug group had extremely significant inhibition rates on the β-Hex release of RBL-2H3 cells (P < 0.01); Comparative Examples 1 to 3, 5 to 7 had significant inhibition rates on the β-Hex release of RBL-2H3 cells (P < 0.05), and there was no significant difference in Comparative Example 4. Compared with the positive drug group, there was no significant difference between Example 1 and the positive drug group (P > 0.05). It is proved that Centella asiatica extract, Chamomile flower extract, grapefruit fruit extract or pomelo fruit extract have good anti-allergic activity, but the effect of using any one extract alone, combining two by two or the ratio of the three extracts not being within the nearest ratio range is weaker than the composition in the ratio of 1:1:3, and the inhibition rate of β-Hex release of RBL-2H3 cells in Example 1 is slightly lower than that of the positive control drug azelastine, which proves that the combination of different extracts has a certain synergistic effect, and the plant composition of Centella asiatica extract, Chamomile flower extract, grapefruit fruit extract or pomelo fruit extract compounded in a mass ratio of 1:1:3 has the most significant effect.

[0070] Example 5 Anti-inflammatory Activity Test

[0071] 1. Experimental Methods

[0072] 1.1 Cell culture

[0073] The cells were placed in a DMEM culture dish and cultured in a 37°C incubator containing 5% CO 2. RAW264.7 macrophages were placed in a 96-well plate and cultured at 37°C for 24 h.

[0074] 1.2 Establishment of LPS-induced inflammatory cell model and detection of pro-inflammatory factors

[0075] RAW264.7 cells were grown at a density of 1 × 10 5 The blank group was not treated, the model group was treated with 1 μg / mL LPS only, and 20 nM dexamethasone was used as the positive control group. In the drug administration group, the samples of Example 1 and Comparative Examples 1 to 7 were added 1 hour in advance for culture, and then 1 μg / mL LPS was added for co-culture for 24 hours. The supernatant was removed and the concentrations of TNF-α and IL-1β were determined using an ELISA kit according to the instructions.

[0076] 2. Experimental results

[0077] LPS is a powerful immunomodulator that activates different pathways in macrophages by acting on cell TLR4. Activated macrophages can produce a large amount of proinflammatory cytokines TNF-α and IL-1β, which in turn induce inflammation. In this way, it becomes crucial to relieve inflammation by reducing the secretion of TNF-α and IL-1β. The blank control group did not cause an increase in the expression of TNF-α and IL-1β. The TNF-α and IL-1β in the model group were significantly higher than those in the blank control group, indicating that the cellular inflammation model was successfully constructed. Compared with the model group, Example 1 and the positive drug had extremely significant inhibition rates on the expression of TNF-α and IL-1β (P < 0.01), and the comparison examples 1 to 7 had significant inhibition rates on the expression of TNF-α and IL-1β (P < 0.05). Compared with the positive drug group, there was no significant difference between Example 1 and the positive drug group (P > 0.05). It is proved that Centella asiatica extract, Chamomile flower extract, grapefruit fruit extract or pomelo fruit extract can reduce the secretion of intracellular pro-inflammatory factors and have good anti-inflammatory effects, but the effect of using any one extract alone, combining two extracts or the three extracts in a ratio not within the nearest ratio range is weaker than the combination in a ratio of 1:1:3. It is proved that the combination of different extracts has a certain synergistic effect, and the plant combination of Centella asiatica extract, Chamomile flower extract, grapefruit fruit extract or pomelo fruit extract in a mass ratio of 1:1:3 has the most significant effect.

[0078] Example 6 Skin barrier repair activity test

[0079] 1. Experimental Methods

[0080] 1.1 Collection of experimental fish and their fertilized eggs

[0081] Sexually mature zebrafish were kept in separate tanks in a zebrafish breeding unit. Water temperature: 26±2℃, pH 7.2, conductivity: 520μs / cm, light / dark cycle: 14h:10h. The day before the exposure experiment, males and females were paired in a ratio of 1:2, mated naturally and spawned, and healthy, deformed and normally developed embryos were randomly selected for the experiment.

[0082] 1.2 Mechanical damage repair test

[0083] Healthy AB zebrafish embryos that developed to 2 dpf (days post fertilization) were selected, the tail fins were cut off, and they were placed in a 6-well cell culture plate, 1 embryo / well, and 5.0 mL of the samples of Example 1 and Comparative Examples 1 to 7 were added respectively. A blank control group was set up in the experiment, and 3 replicates were set up in each group. Observe and take pictures under a microscope, and record the growth of the tail fin 24h, 48h, and 72h after the tail fin was cut off. The zebrafish pictures were analyzed using the image analysis software Image J, and the length of the tail fin growth 24h, 48h, and 72h after the zebrafish tail was cut off was measured, and the repair activity was recorded and analyzed.

[0084] 2. Experimental results

[0085] Compared with the model group at 72 hours after the tail fin was cut off, Example 1 had a very significant growth promotion rate 72 hours after the tail fin was cut off (P ​​< 0.01), and the growth promotion rate of Comparative Examples 1 to 7 was significantly compared with the model group 72 hours after the tail fin was cut off (P ​​< 0.05), indicating that Centella asiatica extract, chamomile flower extract, pomelo fruit extract or grapefruit extract can promote the growth of the tail fin, have a certain degree of tissue damage repair effect, and the combined use of different extracts has a certain synergistic effect, among which Example 1 obtained according to the method for preparing a plant composition provided by the present invention has the most significant effect.

[0086] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A mild soothing and repairing plant composition, characterized in that: The plant composition comprises the following ingredients: Centella asiatica extract, chamomile extract and pomelo extract; wherein the mass ratio of the Centella asiatica extract, the chamomile extract and the pomelo extract is (0.7-1.5): (0.9-1.5): (2-5).

2. The plant composition according to claim 1, characterized in that Among the ingredients, the Centella asiatica extract contains asiatica glycoside and madecassoside in a total amount of ≥3.0%; the chamomile flower extract contains apigenin and cosmosin in a total amount of ≥2.0%; and the pomelo extract contains naringin and neohesperidin in a total amount of ≥5.0%.

3. The plant composition according to any one of claims 1 to 2, characterized in that: The pomelo extract is any one of grapefruit fruit extract and pomelo fruit extract or a combination of both.

4. The method for preparing the plant composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: 1) mixing Centella asiatica extract, chamomile flower extract and grapefruit extract according to proportion; 2) After mixing and stirring evenly, a certain amount of water is added to prepare an extract mixed solution; 3) Centrifuge the prepared mixed solution, take the supernatant and filter it to obtain the product.

5. The method for preparing the plant composition according to claim 4, characterized in that: The mass concentration of the mixed solution of the extract in step 2) is 1-3%.

6. The method for preparing the plant composition according to claim 4, characterized in that: In step 3), the centrifugation condition is 3500-5000 rpm for 25-35 min.

7. Use of the plant composition according to any one of claims 1 to 3 in the preparation of anti-skin allergy products.

8. Use of the plant composition according to any one of claims 1 to 3 in the preparation of skin anti-inflammatory products.

9. Use of the plant composition according to any one of claims 1 to 3 in the preparation of skin barrier repair products.

10. The use according to any one of claims 7 to 9, characterized in that: The products include lotions, ointments, creams, lotions and serums for skin application.

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