Hair growth composition as well as preparation method and application thereof

Abstract

The invention discloses a hair growth composition as well as a preparation method and application thereof, and relates to the technical field of compositions. The invention provides a hair growth composition. The hair growth composition is prepared from the following components in parts by weight: 0.1 to 0.8 part of salmon roe protein peptide, 0.1 to 0.3 part of black rhizoma cimicifugae extract and 0.05 to 0.3 part of spirulina extract. The three raw materials are used for regulating blood circulation, hair follicle regeneration, cell repair and anti-inflammatory response to form a comprehensive mechanism, so that hair growth and scalp health are effectively promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of compositions, in particular to a hair growth composition and a preparation method and application thereof. Background Art

[0002] Non-pathological hair loss or non-excessive hair loss is a normal metabolism. Everyone in life will lose hair. Generally speaking, it is normal for a normal adult to lose 40-70 hairs a day. Losing more than 100 hairs is a sign of hair loss. If you lose more than 100 hairs a day and it lasts for more than 2 or 3 months, you need to see a doctor as soon as possible. Through observations in daily life, it can be concluded that the increase in the number of people with hair loss problems is directly related to the life pressure, living habits, eating habits and changes in the social environment that people bear. Most people with hair loss problems suffer from infectious diseases. Infectious diseases mainly cause hair loss problems by directly destroying hair follicles through infected microorganisms or forming scars that make hair follicles disappear. At present, the most effective way to prevent hair loss problems is to detect the cause early and treat it in a targeted manner.

[0003] Therefore, it is of practical significance and good market prospects to develop a hair growth composition that can promote hair follicle cell proliferation, promote hair growth, and maintain scalp homeostasis. Summary of the invention

[0004] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a hair growth composition that can promote hair follicle cell proliferation, promote hair growth, and maintain scalp homeostasis, as well as a preparation method and application thereof.

[0005] To achieve the above object, the technical solution adopted by the present invention is: a hair growth composition, comprising the following components in parts by weight: 0.1-0.8 parts of salmon roe protein peptide, 0.1-0.3 parts of black cohosh extract, and 0.05-0.3 parts of spirulina extract.

[0006] The salmon roe protein peptide, black cohosh extract and spirulina extract of the present invention target multiple key genes and signal pathways through synergistic action, which can promote hair follicle cell proliferation, promote hair growth and maintain scalp homeostasis. Among them, the salmon roe protein peptide increases VEGF, enhances angiogenesis around hair follicles, improves blood circulation, and provides sufficient oxygen and nutrition for hair follicles; at the same time, the estrogen mimetic substance in the black cohosh extract further increases the expression of VEGF by activating the estrogen receptor (ER); the antioxidant component of the spirulina extract stabilizes VEGF and reduces the impact of oxidative stress. The above three substances work together to increase the expression of VEGF and stabilize VEGF, thereby further promoting hair follicle cell proliferation, promoting hair growth and maintaining scalp homeostasis.

[0007] In addition, salmon roe peptides can promote the regeneration of hair follicles by activating the Wnt / β-catenin signaling pathway; black cohosh extract further activates the pathway through the action of estrogen receptors, and the nutrients in spirulina support the continued activity of this signaling pathway. The three work together to promote hair growth. Salmon roe peptides can also enhance the regeneration and repair of hair follicle cells by activating the IGF-1 signaling pathway; at the same time, black cohosh extract indirectly promotes the activity of IGF-1 by regulating hormone balance, and the key nutrients provided by spirulina further support the function of IGF-1. Spirulina extract can also reduce scalp inflammation and protect hair follicles by inhibiting the NF-κB signaling pathway.

[0008] The present invention regulates blood circulation, hair follicle regeneration, cell repair and anti-inflammatory response through three raw materials, forming a comprehensive mechanism, thereby effectively promoting hair growth and scalp health.

[0009] Preferably, the hair growth composition comprises the following components in parts by weight: 0.2-0.6 parts of salmon roe protein peptide, 0.15-0.2 parts of black cohosh extract, and 0.1-0.15 parts of spirulina extract.

[0010] The inventors found in actual experimental processes that when the weight portions of salmon roe protein peptide, black cohosh extract, and spirulina extract are within the above range, the obtained hair growth composition has better effects on hair follicle cell proliferation, promoting hair growth, and maintaining scalp homeostasis.

[0011] Preferably, the total mass percentage of salmon roe protein peptides in the hair growth composition is 45-65%.

[0012] During the actual experimental process, the inventors found that the total mass percentage of salmon roe protein peptides in the hair growth composition has a great influence on hair follicle cell proliferation, hair growth, and scalp homeostasis. When the total mass percentage of salmon roe protein peptides in the hair growth composition is 45-65%, the obtained hair growth composition has better effects on hair follicle cell proliferation, promoting hair growth, and maintaining scalp homeostasis.

[0013] In addition, the present invention provides the use of the hair growth composition in preparing hair growth granules.

[0014] Furthermore, the present invention provides a hair growth granule, which comprises the following components in parts by weight: 0.25-1.5 parts of the hair growth composition, 0-0.25 parts of an antioxidant, and 0-0.2 parts of an auxiliary material.

[0015] Preferably, the antioxidant is natural juice powder, and the auxiliary material is resistant dextrin; further preferably, the natural juice powder is at least one of strawberry powder, blueberry powder, and lemon powder, and the resistant dextrin is at least one of corn resistant dextrin, potato resistant dextrin, and wheat resistant dextrin.

[0016] In addition, the present invention provides a method for preparing the hair growth granules, comprising the following steps:

[0017] (1) freeze-drying and grinding salmon roe protein peptide;

[0018] (2) drying and crushing the black cohosh extract, spirulina extract, antioxidant and auxiliary materials respectively;

[0019] (3) The materials crushed in step (1) and step (2) are mixed uniformly according to weight to obtain the hair growth granules.

[0020] Preferably, in step (1), the temperature of freeze-drying the salmon roe protein peptide is -80°C to 30°C, and the freeze-drying time is 24-48 hours; the salmon roe protein peptide is crushed to a particle size of 5-50 mm;

[0021] Preferably, in step (2), the drying temperature is 50° C., the drying time is 1-2 h, and the black cohosh extract, spirulina extract, antioxidant and auxiliary materials are respectively crushed to a particle size of 50-100 mm.

[0022] Further preferably, the materials crushed in step (1) and step (2) are mixed evenly by weight, and then ultrafinely crushed to a particle size of 30-200 μm to obtain the hair growth granules.

[0023] Optionally, in actual use, the hair growth granules can be taken with a solvent, wherein the solvent is water or milk, and the temperature of the solvent is 40-50°C.

[0024] Optionally, the mass ratio of the hair growth granules to the solvent is 1 g: (100-200) mL.

[0025] The inventors found in actual experiments that when the temperature of the solvent is 40-50°C, it is more conducive to the dissolution and absorption of fish roe protein peptides and other active ingredients.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: the salmon roe protein peptide, black cohosh extract and spirulina extract of the present invention target multiple key genes and signal pathways through synergistic action, which can promote the proliferation of hair follicle cells, promote hair growth and maintain scalp homeostasis. Among them, the salmon roe protein peptide upregulates VEGF, enhances angiogenesis around hair follicles, improves blood circulation, and provides sufficient oxygen and nutrition for hair follicles; at the same time, the estrogen mimetic substance in the black cohosh extract further increases the expression of VEGF by activating the estrogen receptor (ER); the antioxidant components of the spirulina extract stabilize VEGF and reduce the impact of oxidative stress. The above three substances work together to increase the expression of VEGF and stabilize VEGF, thereby further promoting the proliferation of hair follicle cells, promoting hair growth and maintaining scalp homeostasis.

[0027] In addition, salmon roe peptides can promote the regeneration of hair follicles by activating the Wnt / β-catenin signaling pathway; black cohosh extract further activates the pathway through the action of estrogen receptors, and the nutrients in spirulina support the continued activity of this signaling pathway. The three work together to promote hair growth. Salmon roe peptides can also enhance the regeneration and repair of hair follicle cells by activating the IGF-1 signaling pathway; at the same time, black cohosh extract indirectly promotes the activity of IGF-1 by regulating hormone balance, and the key nutrients provided by spirulina further support the function of IGF-1. Spirulina extract can also reduce scalp inflammation and protect hair follicles by inhibiting the NF-κB signaling pathway.

[0028] The present invention regulates blood circulation, hair follicle regeneration, cell repair and anti-inflammatory response through three raw materials, forming a comprehensive mechanism, thereby effectively promoting hair growth and scalp health. DETAILED DESCRIPTION

[0029] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0030] The raw materials used in the present invention are described as follows:

[0031] Salmon roe protein peptide: Shanghai Chengyi Health Technology Group Co., Ltd.;

[0032] Black cohosh extract: Shaanxi Tianxingjian Biochemical Technology Co., Ltd.;

[0033] Spirulina extract: Qingdao Algae Blue Biological Co., Ltd.;

[0034] Salmon oligopeptide: 500Da model JGT-078, Shaanxi Jiaguo Peptide Bioengineering Co., Ltd.;

[0035] Soybean isoflavone extract: content 20%, Henan Tianxing Food Additive Co., Ltd.;

[0036] Zinc amino acid chelate: Henan Haizhiyuan Food Additive Co., Ltd.;

[0037] Natural juice powder: Tuofeng Powder Technology (Kunshan) Co., Ltd., lemon powder;

[0038] Resistant dextrin: Shanghai Songgu International Trade Co., Ltd., corn resistant dextrin.

[0039] Examples and Comparative Examples

[0040] The invention provides a hair growth granule. The components and weight portions of the hair growth granule are selected as shown in Table 1-Table 2. The preparation method of the hair growth granule comprises the following steps: (1) freeze-drying, grinding and crushing salmon roe protein peptide; (2) drying and crushing black cohosh extract, spirulina extract, antioxidant and auxiliary materials respectively; (3) mixing the materials crushed in step (1) and step (2) uniformly according to weight portions to obtain the hair growth granule.

[0041] Among them, the freezing temperature is -40℃ to -80℃, the freezing time is 12 hours, the low-pressure sublimation stage temperature is -20℃ to -40℃, the time is 12 hours, the post-drying stage temperature is 20℃ to 30℃, the time is 24 hours, the condenser temperature is -50℃ to -80℃, and the heating rate is 1℃ / hour to 5℃.

[0042] The preparation methods of the embodiments and comparative examples are the same, and components that need to be replaced can be replaced, and those that do not have relevant components are not added.

[0043] Table 1

[0044]

[0045] Table 2

[0046]

[0047] Performance Test-1 Hair Follicle Cell Proliferation Test

[0048] Objective: To evaluate the effect on hair follicle cell proliferation and verify its potential to promote hair growth. Human hair follicle cell line hair matrix cells were selected and cultured in DMEM medium containing 10% fetal bovine serum. Hair follicle cells cultured to the logarithmic growth phase were cultured at 1×10 5 The cells were seeded at a density of 10 cells / well in a 96-well plate and cultured at 37°C and 5% CO2 for 24 hours to ensure cell adhesion. After 24 hours, the original culture medium was removed, and the hair growth granules prepared in the example at a ratio of 1g:100mL warm water were added respectively. An equal volume of deionized water was added to the control group, and the culture was continued for 24 hours. After the treatment, the CCK-8 method was used to detect the proliferation of the cells. The OD value was measured at a wavelength of 450nm using an enzyme marker. The cell proliferation was evaluated by the change in the OD value. Each group of experiments was repeated at least three times, and the average OD value and standard error of each group were recorded. Cell activity = (OD 样 -OD 空 ) / (OD 对 -OD 空 )×100%.

[0049] Experimental group (containing hair growth granules and hair follicle cells), control group (deionized water and hair follicle cells), blank group (hair growth granules and no hair follicle cells).

[0050] The products prepared in the examples and comparative examples were prepared into hair growth granules at a ratio of 1 g: 100 mL of warm water, and deionized water was set as a control group. The experimental results are shown in the following table.

[0051] Table 3

[0052]

[0053] As can be seen from the above table, the hair growth composition prepared in the embodiment of the present invention, salmon roe protein peptide, black cohosh extract and spirulina extract can promote hair follicle cell proliferation, promote hair growth and maintain scalp homeostasis by synergistically targeting multiple key genes and signal pathways.

[0054] Performance Test-2 Hair Growth Test and Scalp Health Assessment

[0055] Refer to the conventional hair growth experiment operation process. Select 30 volunteers aged between 18 and 60 with healthy scalp and no serious hair loss problems; the subjects should have no history of allergies or other medical conditions that affect hair growth.

[0056] The hair growth granules prepared in the examples and comparative examples were prepared, and the hair growth products were prepared by mixing the products prepared in the examples and comparative examples (g): warm water (mL) at a configuration ratio of 1:100; the control group used deionized water.

[0057] Before the experiment, the hair density of the subjects' specific scalp areas (such as the top of the head or the temples) was measured, and the initial state of the hair was recorded using standardized photography. The experimental group consumed the product in the prescribed amount every day (1 pack each morning and before going to bed, 1g per pack, and mixed with 100mL of warm water), and the control group drank water. The continuous use period was 3 months. Hair growth was evaluated after 0, 1, and 3 months, including measuring hair density, length, diameter and other indicators, and taking photos for record. A hair analyzer was used to evaluate changes in the number of hairs per square centimeter of scalp. A precision ruler was used to measure the length of hair growth. The hair growth of the experimental group and the control group was compared to evaluate the effect of the hair growth granules on hair growth.

[0058] Note: 1. During the experiment, the subjects should avoid using other hair care products to avoid interfering with the test results. 2. During the experiment, the hair analyzer must be used at the same time. Evaluate hair growth status to ensure data accuracy and comparability.

[0059] The experimental results are shown in Tables 4-5 below.

[0060] Scalp Health Assessment

[0061] The volunteers for the scalp health assessment and the hair growth test were from the same group, aged between 18 and 60 years old, with healthy scalps and no serious hair loss problems.

[0062] (1) Scalp moisture content; Tool: Skin moisture tester CM 825; Purpose: To evaluate the moisturizing effect of the product and ensure that the moisture level of the scalp is maintained or improved after using the product. Method: Measure the moisture content of different areas of the scalp.

[0063] (2) Scalp oil secretion; Tools: Sebum secretion tester SM 815; Purpose: To evaluate the oil control effect of the product and determine whether it is effective in reducing scalp oil secretion. Method: Measure the oil secretion of the scalp.

[0064] (3) Scalp pH; Tools: pH tester. Purpose: To monitor the pH of the scalp and ensure that it is maintained within the normal range (pH 4.5-5.5) to avoid discomfort or irritation. Method: To measure the pH of the scalp to evaluate the effect of the product on pH balance.

[0065] The scalp pH values ​​of the embodiments of the present invention and the comparative examples were maintained within the normal range (pH 4.5-5.5), which are not listed here one by one.

[0066] The experimental results are shown in Tables 6-7 below.

[0067] Table 4

[0068]

[0069]

[0070] Table 5

[0071]

[0072]

[0073] Table 6

[0074]

[0075] Table 7

[0076]

[0077]

[0078] As can be seen from the above table, the hair growth composition prepared in the embodiment of the present invention, the salmon roe protein peptide, the black cohosh extract and the spirulina extract can target multiple key genes and signal pathways through synergistic effects, and can promote hair growth, reduce the oil secretion of the scalp, increase the moisture content of the scalp, and maintain the homeostasis of the scalp.

[0079] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A hair growth composition, characterized in that: The invention comprises the following components in parts by weight: 0.1-0.8 parts of salmon roe protein peptide, 0.1-0.3 parts of black cohosh extract and 0.05-0.3 parts of spirulina extract.

2. The hair growth composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 0.2-0.6 parts of salmon roe protein peptide, 0.15-0.2 parts of black cohosh extract and 0.1-0.15 parts of spirulina extract.

3. The hair growth composition according to claim 1, characterized in that The total mass percentage of salmon roe protein peptide in the hair growth composition is 45-65%.

4. Use of the hair growth composition according to any one of claims 1 to 3 in the preparation of hair growth granules.

5. A hair growth granule, characterized in that: The hair growth granule comprises the following components in parts by weight: 0.25-1.5 parts of the hair growth composition according to any one of claims 1 to 4, 0-0.25 parts of an antioxidant, and 0-0.2 parts of an auxiliary material.

6. The hair growth granule according to claim 5, characterized in that The antioxidant is natural fruit juice powder, and the auxiliary material is resistant dextrin.

7. The method for preparing the hair growth granules according to claim 5, characterized in that: The following steps are involved: (1) freeze-drying and crushing salmon roe protein peptide; (2) drying and crushing the black cohosh extract, spirulina extract, antioxidant and auxiliary materials respectively; (3) The materials crushed in step (1) and step (2) are mixed uniformly according to weight to obtain the hair growth granules.

8. The method for preparing the hair growth granules according to claim 7, characterized in that: In the step (3), the materials crushed in the steps (1) and (2) are mixed evenly by weight, and then ultrafinely crushed to a particle size of 30-200 μm to obtain the hair growth granules.

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