Health preserving pill for improving immunity and preventing tumors and preparation method thereof

Abstract

The invention relates to a health preserving pill capable of improving immunity and preventing tumors and a preparation method of the health preserving pill, and relates to the technical field of traditional Chinese medicine. The functional components comprise the following raw materials: 15-25 parts of endothelium corneum gigeriae galli, 5-10 parts of codonopsis pilosula, 10-18 parts of astragalus membranaceus, 3-7 parts of ginseng, 8-15 parts of phellinus igniarius, 15-25 parts of towel gourd seeds, 30-40 parts of cucumber seeds, 18-30 parts of raw gardenia and 7-12 parts of soft-shelled turtle protein peptide. According to the health-preserving pill provided by the invention, a plurality of traditional Chinese medicinal materials and bioactive substances with immunoregulation and anti-tumor effects are selected and comprehensively act on an immune system and tumor cells of a body, so that the effects of improving immunity and preventing tumors are jointly achieved, and a safe, efficient and comprehensive health-preserving health-care product is provided for consumers; the positive significance is realized on improving the body immunity, preventing tumors and promoting the human health.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a health-preserving pill for improving immunity and preventing tumors and a preparation method thereof. Background Art

[0002] With the rapid development of modern society, people's pace of life is accelerating, and problems such as environmental pollution and bad living habits are becoming increasingly prominent, leading to a decline in human immunity and an increase in the incidence of tumors year by year. In this context, how to effectively improve human immunity and prevent tumors has become the focus of current medical research and public attention.

[0003] Traditional Chinese medicine has a profound theoretical foundation and rich practical experience in health preservation and disease prevention. Chinese medicine emphasizes "preventive treatment", that is, preventing the occurrence of diseases by conditioning the body and strengthening the constitution. In Chinese medicine theory, the strength of human immunity is closely related to the rise and fall of qi and blood and the functional state of internal organs. Therefore, health products made from Chinese medicine formulas, such as health pills, have become one of the important means to improve immunity and prevent tumors.

[0004] There are many health products on the market that can improve immunity or prevent tumors, but these products often have single functions, unclear effects or large side effects. Therefore, it is of great significance to develop a health product that can significantly improve immunity, effectively prevent tumors, and is safe and has no side effects, whether for cancer patients or people with low immunity such as the frail elderly. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a health-preserving pill for improving immunity and preventing tumors and a preparation method thereof.

[0006] In a first aspect, the present invention provides a health-preserving pill for improving immunity and preventing tumors, the health-preserving pill comprising effective ingredients and auxiliary materials, wherein the effective ingredients comprise the following raw materials in parts by weight:

[0007] 15-25 parts of chicken gizzard lining, 5-10 parts of codonopsis pilosula, 10-18 parts of astragalus, 3-7 parts of ginseng, 8-15 parts of mulberry linterus, 15-25 parts of loofah seeds, 30-40 parts of cucumber seeds, 18-30 parts of raw gardenia, and 7-12 parts of soft-shelled turtle protein peptide.

[0008] Furthermore, the functional ingredients include the following raw materials in parts by weight:

[0009] 19 parts of chicken's gizzard lining, 8 parts of codonopsis pilosula, 14.5 parts of astragalus, 5 parts of ginseng, 10 parts of phellinus linteus, 20 parts of loofah seeds, 35 parts of cucumber seeds, 23 parts of raw gardenia, and 9.5 parts of soft-shelled turtle protein peptide.

[0010] Furthermore, the auxiliary material includes honey; the weight ratio of the honey to the effective ingredient is (0.9-1.2):(1-1.5).

[0011] In a second aspect, the present invention provides a method for preparing the health-preserving pill for improving immunity and preventing tumors as described in any one of the first aspects, the preparation method comprising the following steps:

[0012] Mix and crush Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius, and then perform supercritical extraction to obtain a first extract;

[0013] Adding chicken gizzard lining, loofah seeds, cucumber seeds and raw gardenia to cyclohexane for reflux extraction to obtain a second extract;

[0014] Mixing the first extract, the second extract and soft-shell turtle protein peptide to obtain the effective ingredient;

[0015] The effective ingredients and auxiliary materials are mixed and then made into pills to obtain the health-preserving pills.

[0016] Furthermore, the working condition parameters of the supercritical extraction include: using a supercritical carbon dioxide fluid with a flow rate of 25 to 30 kg / h as a supercritical extraction medium, a supercritical extraction pressure of 55 to 60 MPa, a supercritical extraction temperature of 50 to 60° C., and a supercritical extraction time of 1 to 3 hours.

[0017] Furthermore, the supercritical extraction pressure is 57 MPa, and the supercritical extraction temperature is 56°C.

[0018] Furthermore, the working condition parameters of the reflux extraction include: the reflux extraction temperature is 85 to 95° C., the reflux extraction time is 1 to 3 hours, and the reflux extraction times are 1 to 3 times.

[0019] Furthermore, the added weight of the cyclohexane is (5 to 10) times the total weight of the chicken gizzard lining, loofah seeds, cucumber seeds and raw gardenia.

[0020] Furthermore, the pill making method is a molding method.

[0021] The above technical solution provided by the present invention has at least the following advantages compared with the prior art:

[0022] The present invention provides a health-preserving pill for improving immunity and preventing tumors. The health-preserving pill provided by the present invention selects a variety of Chinese medicinal materials and bioactive substances with immune regulation and anti-tumor effects, and acts on the body's immune system and tumor cells in a comprehensive manner, thereby jointly improving immunity and preventing tumors. The purpose is to provide consumers with a safe, efficient, and comprehensive health and wellness solution, which has positive significance for improving body immunity, preventing tumors, and promoting human health. Specifically:

[0023] 1) Enhance immunity: Codonopsis, Astragalus and Ginseng in the formula are all commonly used traditional Chinese medicine herbs for invigorating qi and strengthening the body, which can enhance the body's immune function and improve the body's resistance. Chicken gizzard lining is a good medicine for strengthening the spleen and digestion, which helps to improve the spleen and stomach function, thereby promoting nutrient absorption, ensuring that the effective ingredients in the health pills can be fully utilized and providing sufficient energy for the immune system. As a high-quality protein source that is easy to absorb, turtle protein peptides can also provide necessary support for the construction and repair of the immune system.

[0024] 2) Prevent tumors: As a rare medicinal fungus, Phellinus igniarius has significant anti-tumor activity, can inhibit tumor cell growth, promote tumor cell apoptosis; improve the body's metabolic state, reduce the accumulation of toxins and harmful substances, and provide a good internal environment for the normal function of immune cells. In traditional medicine, loofah seeds and cucumber seeds are believed to have the effects of clearing heat and detoxifying, diuresis and detoxification, which help to remove harmful substances in the body and reduce the risk of tumors. Raw gardenia is rich in antioxidants, which can remove free radicals in the body, reduce the damage of oxidative stress to cells, protect immune cells and other important cells from damage, and help prevent symptoms such as inflammation caused by tumors.

[0025] 3) Synergistic enhancement and comprehensive conditioning: The various active ingredients interact with each other to form a synergistic effect, which not only aims at improving immunity and preventing tumors, but also takes into account spleen and stomach conditioning, clearing away heat and detoxification, diuresis and detoxification, etc., to achieve comprehensive conditioning of the body. This comprehensive health-preserving effect helps to improve the overall health level and reduce the occurrence of diseases.

[0026] 4) Safe and natural: All raw materials are derived from nature, avoiding the side effects that may be caused by chemical synthetic ingredients. It is safer and more reliable, suitable for long-term use, and in line with modern people's pursuit of natural and healthy health concepts.

[0027] In summary, the health-preserving pills, through the scientific proportion of natural effective ingredients, on the one hand, enhance the overall physiological function of the body by replenishing qi and blood, regulating the balance of the internal environment, and on the other hand, directly act on the immune system and tumor cells through anti-oxidation, scavenging free radicals, and promoting nutrient absorption, so as to achieve the goal of improving immunity and preventing tumors. It can not only effectively improve human immunity and prevent tumors, but also achieve comprehensive conditioning of the body. It is a safe and effective health care product. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described in combination with the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0030] In a first aspect, the present invention provides a health-preserving pill for improving immunity and preventing tumors, the health-preserving pill comprising effective ingredients and auxiliary materials, wherein the effective ingredients comprise the following raw materials in parts by weight:

[0031] 15-25 parts of chicken gizzard lining, 5-10 parts of codonopsis pilosula, 10-18 parts of astragalus, 3-7 parts of ginseng, 8-15 parts of mulberry linterus, 15-25 parts of loofah seeds, 30-40 parts of cucumber seeds, 18-30 parts of raw gardenia, and 7-12 parts of soft-shelled turtle protein peptide.

[0032] The present invention provides a health-preserving pill for improving immunity and preventing tumors. The health-preserving pill provided by the present invention selects a variety of Chinese medicinal materials and bioactive substances with immune regulation and anti-tumor effects, and acts on the body's immune system and tumor cells in a comprehensive manner, thereby jointly improving immunity and preventing tumors. The purpose is to provide consumers with a safe, efficient, and comprehensive health and wellness solution, which has positive significance for improving body immunity, preventing tumors, and promoting human health. Specifically:

[0033] 1) Enhance immunity: Codonopsis, Astragalus and Ginseng in the formula are all commonly used traditional Chinese medicine herbs for invigorating qi and strengthening the body, which can enhance the body's immune function and improve the body's resistance. Chicken gizzard lining is a good medicine for strengthening the spleen and digestion, which helps to improve the spleen and stomach function, thereby promoting nutrient absorption, ensuring that the effective ingredients in the health pills can be fully utilized and providing sufficient energy for the immune system. As a high-quality protein source that is easy to absorb, turtle protein peptides can also provide necessary support for the construction and repair of the immune system.

[0034] 2) Prevent tumors: As a rare medicinal fungus, Phellinus igniarius has significant anti-tumor activity, can inhibit tumor cell growth, promote tumor cell apoptosis; improve the body's metabolic state, reduce the accumulation of toxins and harmful substances, and provide a good internal environment for the normal function of immune cells. In traditional medicine, loofah seeds and cucumber seeds are believed to have the effects of clearing heat and detoxifying, diuresis and detoxification, which help to remove harmful substances in the body and reduce the risk of tumors. Raw gardenia is rich in antioxidants, which can remove free radicals in the body, reduce the damage of oxidative stress to cells, protect immune cells and other important cells from damage, and help prevent symptoms such as inflammation caused by tumors.

[0035] 3) Synergistic enhancement and comprehensive conditioning: The various active ingredients interact with each other to form a synergistic effect, which not only aims at improving immunity and preventing tumors, but also takes into account spleen and stomach conditioning, clearing away heat and detoxification, diuresis and detoxification, etc., to achieve comprehensive conditioning of the body. This comprehensive health-preserving effect helps to improve the overall health level and reduce the occurrence of diseases.

[0036] 4) Safe and natural: All raw materials are derived from nature, avoiding the side effects that may be caused by chemical synthetic ingredients. It is safer and more reliable, suitable for long-term use, and in line with modern people's pursuit of natural and healthy health concepts.

[0037] In summary, the health-preserving pills, through the scientific proportion of natural effective ingredients, on the one hand, enhance the overall physiological function of the body by replenishing qi and blood, regulating the balance of the internal environment, and on the other hand, directly act on the immune system and tumor cells through anti-oxidation, scavenging free radicals, and promoting nutrient absorption, so as to achieve the goal of improving immunity and preventing tumors. It can not only effectively improve human immunity and prevent tumors, but also achieve comprehensive conditioning of the body. It is a safe and effective health care product.

[0038] The following is a detailed introduction to chicken gizzard lining, codonopsis, astragalus, ginseng, mulberry, loofah seeds, cucumber seeds, raw gardenia and turtle protein peptide:

[0039] Chicken gizzard lining: Other names: chicken gizzard skin, chicken yellow skin, chicken gizzard, chicken gizzard; Nature and flavor: sweet, cold. Enter the spleen, stomach, small intestine, bladder meridian; Source: the dried gizzard lining (muscular stomach) of domestic chickens; Efficacy and function: Digestion and stomach-strengthening: Chicken gizzard lining contains gastric hormones, keratin and other ingredients, which can promote gastric juice secretion, increase gastric acidity and digestion, and accelerate gastric emptying rate. Astringent and stop nocturnal emission: often used in combination with other medicinal materials to treat nocturnal enuresis, nocturnal emission and frequent urination. 3. To relieve urinary tract stones: commonly used in modern clinical practice to treat urinary tract stones and gallstones. Other effects: such as the treatment of female amenorrhea.

[0040] Codonopsis pilosula: Scientific name: Codonopsis pilosula (Franch.) Nannf.; Family: Campanulaceae Codonopsis genus perennial herbaceous vine; Nature and flavor: sweet, neutral; enters the spleen and lung meridians; Efficacy and function: Strengthen the spleen and lungs: used to treat symptoms such as spleen and stomach weakness, insufficient qi, and lung qi deficiency. Nourishing blood and promoting body fluid: It has the effects of nourishing blood and promoting body fluid to quench thirst. Enhance immunity: Modern pharmacological research shows that Codonopsis pilosula also has the effect of enhancing the body's immunity.

[0041] Astragalus: Scientific name: Astragalus membranaceus (Fisch.) Bunge; Family: Leguminosae; Nature and flavor: sweet, slightly warm; enters the spleen and lung meridians; Functions: tonifying qi and raising yang: used to treat symptoms such as qi deficiency, fatigue, and sinking of qi in the middle. Strengthening the exterior and stopping sweating: has antiperspirant effect, suitable for patients with spontaneous sweating due to exterior deficiency. Promoting diuresis and reducing edema: can be used to treat symptoms such as edema. Other functions: such as promoting fluid and nourishing blood, relieving stagnation and relieving numbness, etc.

[0042] Ginseng: Scientific name: Panax ginseng CAMey; Family: Araliaceae, Panax genus, perennial herb; Nature and flavor: sweet, slightly bitter, slightly warm; enters the spleen, lung, and heart meridians; Functions and effects: Greatly replenishes vitality: Ginseng is a famous tonic herb that can greatly replenish vitality. Nourishes the spleen and lungs: Used to treat symptoms such as weak spleen and stomach, insufficient lung qi, etc. Promotes the production of body fluids and quenches thirst: It has the effect of promoting the production of body fluids and quenching thirst, and is suitable for patients with internal heat and thirst. Calms the mind and improves intelligence: Helps improve sleep quality and intelligence.

[0043] Phellinus igniarius: Family: The fruiting body of the fungus Phellinus igniarius of the Polyporaceae family; Nature and flavor: Slightly bitter, cold in nature; Enters the liver and kidney meridians; Effects and functions: Activating blood circulation and stopping bleeding: It has the effects of activating blood circulation, removing blood stasis and stopping bleeding. Eliminating phlegm and stopping diarrhea: It can be used to treat symptoms such as spleen deficiency and diarrhea. Anti-tumor: Modern pharmacological studies have shown that Phellinus igniarius has anti-tumor effects.

[0044] Luffa seeds: have antibacterial, anti-cancer and anti-cancer effects, and can also be used to treat symptoms such as double moth wind.

[0045] Cucumber seeds: Rich in nutrients such as calcium, phosphorus, iron, etc., which help promote bone growth and repair.

[0046] Raw Gardenia: It has the effects of clearing away heat and relieving restlessness, clearing away heat and dampness, cooling blood and detoxifying. It is often used to treat symptoms such as restlessness caused by fever, damp-heat jaundice, etc.

[0047] Turtle protein peptide: also known as turtle peptide, is a protein hydrolyzate extracted from turtles, and has multiple biological activities such as enhancing immunity and promoting wound healing. In some specific embodiments, commercially available turtle protein peptides such as Guangdong Hengtian Biotechnology Co., Ltd., food grade, can be directly used.

[0048] In some specific embodiments, the functional ingredients include the following raw materials in parts by weight:

[0049] 19 parts of chicken's gizzard lining, 8 parts of codonopsis pilosula, 14.5 parts of astragalus, 5 parts of ginseng, 10 parts of phellinus linteus, 20 parts of loofah seeds, 35 parts of cucumber seeds, 23 parts of raw gardenia, and 9.5 parts of soft-shelled turtle protein peptide.

[0050] In some specific embodiments, the auxiliary material includes honey; the weight ratio of the honey to the effective ingredient is (0.9-1.2):(1-1.5).

[0051] It should be noted that, unless otherwise specified or specified, the components and raw materials involved in the health-preserving pills for improving immunity and preventing tumors provided by the present invention can be directly commercially available products or homemade using existing preparation processes.

[0052] In a second aspect, based on a general inventive concept, the present invention provides a method for preparing the health-preserving pills for improving immunity and preventing tumors as described in any one of the first aspects, the preparation method comprising the following steps:

[0053] Mix and crush Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius, and then perform supercritical extraction to obtain a first extract;

[0054] Adding chicken gizzard lining, loofah seeds, cucumber seeds and raw gardenia to cyclohexane for reflux extraction to obtain a second extract;

[0055] Mixing the first extract, the second extract and soft-shell turtle protein peptide to obtain the effective ingredient;

[0056] The effective ingredients and auxiliary materials are mixed and then made into pills to obtain the health-preserving pills.

[0057] The preparation method of the health-preserving pills for improving immunity and preventing tumors provided by the present invention is simple to operate, does not require additional specific equipment, and is suitable for industrial batch production.

[0058] In some specific embodiments, the working condition parameters of the supercritical extraction include: using a supercritical carbon dioxide fluid with a flow rate of 25 to 30 kg / h as a supercritical extraction medium, a supercritical extraction pressure of 55 to 60 MPa, a supercritical extraction temperature of 50 to 60°C, and a supercritical extraction time of 1 to 3 hours.

[0059] In some specific embodiments, the supercritical extraction pressure is 57 MPa and the supercritical extraction temperature is 56°C.

[0060] In some specific embodiments, the working condition parameters of the reflux extraction include: the reflux extraction temperature is 85-95° C., the reflux extraction time is 1-3 hours, and the number of reflux extractions is 1-3 times.

[0061] In some specific embodiments, the added weight of the cyclohexane is (5-10) times the total weight of the chicken gizzard lining, loofah seeds, cucumber seeds and raw gardenia.

[0062] In some specific embodiments, the pill making method is a molding method. The molding method is a commonly used method for preparing Chinese medicine pills in the art, which has the advantages of simple process, convenient operation, and stable quality of finished products. The following are the detailed steps and condition parameters for making pills of the functional ingredients and honey in the present invention by the molding method to obtain health-preserving pills: mixing stage: mixing time is 10-30 minutes; molding machine parameters: controlled between 5-20MPa, time is 1-5 minutes; drying: controlled between 40-80℃, time is 12 to 24 hours.

[0063] It should be noted that the operating steps involved in the preparation method of the health-preserving pills for improving immunity and preventing tumors provided by the present invention, unless otherwise specified or explained, such as filtering, drying and pilling, etc., can be carried out according to the existing processes in the art or using existing equipment, and will not be described one by one here.

[0064] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0065] Example 1

[0066] This example provides a health-preserving pill for improving immunity and preventing tumors. The health-preserving pill includes effective ingredients and auxiliary materials. In parts by weight, the effective ingredients are composed of the following raw materials:

[0067] Chicken gizzard 19 parts, Codonopsis pilosula 8 parts, Astragalus 14.5 parts, Ginseng 5 parts, Phellinus linteus 10 parts, Luffa seeds 20 parts, Cucumber seeds 35 parts, Gardenia jasminoides 23 parts, Turtle protein peptide 9.5 parts;

[0068] The auxiliary material is honey; the weight ratio of the honey to the effective ingredient is 1:1.2.

[0069] The preparation method of the above-mentioned health-preserving pills for improving immunity and preventing tumors comprises the following steps:

[0070] Step (1): mixing and grinding Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius, and adding the mixture into a supercritical device for supercritical extraction, using a supercritical carbon dioxide fluid with a flow rate of 28 kg / h as a supercritical extraction medium, a supercritical extraction pressure of 57 MPa, a supercritical extraction temperature of 56° C., and a supercritical extraction time of 1 hour, then centrifuging the extracted product to remove the bottom precipitate, and evacuating the supernatant in a vacuum box at a temperature of 45° C. for 12 hours to obtain a first extract;

[0071] Step (2): adding chicken's gizzard lining, loofah seeds, cucumber seeds and raw gardenia to cyclohexane for reflux extraction, the reflux extraction temperature is 90° C., the reflux extraction time is 1.5 hours, the reflux extraction number is 2 times, and the added weight of the cyclohexane is 6 times the total weight of the chicken's gizzard lining, loofah seeds, cucumber seeds and raw gardenia; filtering, combining the obtained extracts and concentrating and drying to constant weight to obtain a second extract;

[0072] Step (3): mixing the first extract obtained in step (1), the second extract obtained in step (2) and the turtle protein peptide to obtain the effective component;

[0073] Step (4): mixing the effective ingredients obtained in step (3) with auxiliary materials and making pills to obtain the health-preserving pills.

[0074] Example 2

[0075] This example provides a health-preserving pill for improving immunity and preventing tumors. The health-preserving pill includes effective ingredients and auxiliary materials. In parts by weight, the effective ingredients are composed of the following raw materials:

[0076] 15 parts of chicken's gizzard lining, 5 parts of codonopsis pilosula, 10 parts of astragalus, 3 parts of ginseng, 8 parts of phellinus linteus, 15 parts of loofah seeds, 30 parts of cucumber seeds, 18 parts of raw gardenia, 7 parts of soft-shelled turtle protein peptide;

[0077] The auxiliary material is honey; the weight ratio of the honey to the effective ingredient is 1:1.2.

[0078] The preparation method of the above-mentioned health-preserving pills for improving immunity and preventing tumors comprises the following steps:

[0079] Step (1): after mixing and grinding Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius, the mixture is added into a supercritical device for supercritical extraction, a supercritical carbon dioxide fluid with a flow rate of 25 kg / h is used as a supercritical extraction medium, a supercritical extraction pressure of 55 MPa, a supercritical extraction temperature of 50° C., and a supercritical extraction time of 1 hour, the extracted product is then centrifuged to remove the bottom precipitate, and the supernatant is evacuated in a vacuum box at a temperature of 45° C. for 12 hours to obtain a first extract;

[0080] Step (2): adding chicken's gizzard lining, loofah seeds, cucumber seeds and raw gardenia to cyclohexane for reflux extraction, the reflux extraction temperature is 90° C., the reflux extraction time is 1.5 hours, the reflux extraction number is 2 times, and the added weight of the cyclohexane is 6 times the total weight of the chicken's gizzard lining, loofah seeds, cucumber seeds and raw gardenia; filtering, combining the obtained extracts and concentrating and drying to constant weight to obtain a second extract;

[0081] Step (3): mixing the first extract obtained in step (1), the second extract obtained in step (2) and the turtle protein peptide to obtain the effective component;

[0082] Step (4): mixing the effective ingredients obtained in step (3) with auxiliary materials and making pills to obtain the health-preserving pills.

[0083] Example 3

[0084] This example provides a health-preserving pill for improving immunity and preventing tumors. The health-preserving pill includes effective ingredients and auxiliary materials. In parts by weight, the effective ingredients are composed of the following raw materials:

[0085] Chicken's gizzard lining 25 parts, Codonopsis pilosula 10 parts, Astragalus 18 parts, Ginseng 7 parts, Phellinus linteus 15 parts, Loofah seeds 25 parts, Cucumber seeds 40 parts, Gardenia jasminoides 30 parts, Turtle protein peptide 12 parts;

[0086] The auxiliary material is honey; the weight ratio of the honey to the effective ingredient is 1:1.2.

[0087] The preparation method of the above-mentioned health-preserving pills for improving immunity and preventing tumors comprises the following steps:

[0088] Step (1): mixing and grinding Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius, and adding them into a supercritical device for supercritical extraction, using a supercritical carbon dioxide fluid with a flow rate of 25 kg / h as a supercritical extraction medium, a supercritical extraction pressure of 60 MPa, a supercritical extraction temperature of 60° C., and a supercritical extraction time of 1 hour, then centrifuging the extracted product to remove the bottom precipitate, and evacuating the supernatant in a vacuum box at a temperature of 45° C. for 12 hours to obtain a first extract;

[0089] Step (2): adding chicken's gizzard lining, loofah seeds, cucumber seeds and raw gardenia to cyclohexane for reflux extraction, the reflux extraction temperature is 90° C., the reflux extraction time is 1.5 hours, the reflux extraction number is 2 times, and the added weight of the cyclohexane is 6 times the total weight of the chicken's gizzard lining, loofah seeds, cucumber seeds and raw gardenia; filtering, combining the obtained extracts and concentrating and drying to constant weight to obtain a second extract;

[0090] Step (3): mixing the first extract obtained in step (1), the second extract obtained in step (2) and the turtle protein peptide to obtain the effective component;

[0091] Step (4): mixing the effective ingredients obtained in step (3) with auxiliary materials and making pills to obtain the health-preserving pills.

[0092] Comparative Example 1

[0093] This example provides a health-preserving pill and a preparation method thereof, which differs from Example 1 only in that: the supercritical extraction in step (1) is performed by alcohol extraction, and the specific alcohol extraction is as follows: after mixing and grinding Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius, the mixture is added into 95% ethanol by volume for reflux extraction, the reflux extraction temperature is 90°C, the reflux extraction time is 1.5 hours, the reflux extraction times are 2 times, and the added weight of the ethanol is 6 times the total weight of the Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius; the obtained extract is filtered, combined and concentrated to constant weight to obtain a first extract; the remaining steps and parameters are the same.

[0094] Comparative Example 2

[0095] This example provides a health-preserving pill and a preparation method thereof, which differs from Example 1 only in that the solvent for reflux extraction in step (2) is adjusted to ethanol with a volume fraction of 95%; the remaining steps and parameters are the same.

[0096] Test Case

[0097] In this example, the effects of the health-preserving pills provided in Examples 1 to 3 and Comparative Examples 1 to 2 were tested.

[0098] In order to evaluate the immune improvement effect of the health-preserving pills provided in Examples 1 to 3 and Comparative Examples 1 to 2 on cyclophosphamide-induced immunosuppressed mice, the following process is specifically included:

[0099] 1. Experimental Animals

[0100] Animal species and number: 70 qualified Km mice were selected and randomly divided into 7 groups, with 10 mice in each group, half of which were male and half were female.

[0101] Weight range: The weight of mice was controlled between 20-22g.

[0102] 2. Grouping and processing

[0103] Normal control group: did not receive any drug treatment and served as a baseline reference.

[0104] Model group: only received cyclophosphamide treatment to establish an immunocompromised model.

[0105] Experimental group: divided into five groups, and gavaged with the health-preserving pills provided by Example 1, Example 2, Example 3, Comparative Example 1, and Comparative Example 2 respectively.

[0106] Dosage and frequency: Once a day, 2g / kg body weight each time, for 7 consecutive days.

[0107] Cyclophosphamide treatment: On the second day after administration, mice in all groups except the normal control group were intraperitoneally injected (ip) with cyclophosphamide at a dose of 100 mg / kg.

[0108] 3. Index detection

[0109] Time point: 1 hour after the last dose.

[0110] Body weight measurement: The body weight of each mouse was recorded.

[0111] Sample collection: Mice were sacrificed by cervical dislocation and the thymus was removed by rapid autopsy.

[0112] Calculation of thymus coefficient: The thymus weight (mg) was divided by the mouse body weight (g) to obtain the thymus coefficient, which was used as one of the indicators for evaluating immune function.

[0113] 4. Data Collection and Analysis

[0114] The thymus coefficient data of all mice were collected, the differences between the groups were compared, and the effects of each health-preserving pill on immune function were evaluated; the test results are shown in Table 1.

[0115] Table 1

[0116] Group Thymus index (mg / g) Normal control group 3.82 Model Group 1.14 Example 1 Group 2.79 Example 2 Group 2.01 Example 3 Group 2.35 Comparative Example 1 1.68 Comparative Example 2 1.37

[0117] It can be seen from Table 1 that: 1) compared with the normal control group, the thymus coefficient of the model group was significantly reduced, indicating that an immunosuppression model was formed; 2) compared with the model group, the thymus coefficient of the mice taking the health-preserving pills provided by Examples 1 to 3 of the present invention was significantly increased, indicating that the immune function of immunosuppressed mice can be improved, and the effect of improving the immune function of immunosuppressed mice caused by cyclophosphamide is significantly better than that of Comparative Examples 1 to 2.

[0118] In addition, based on the above test results, according to the test method disclosed in the prior art, the inhibitory effect of the health-preserving pills provided in Examples 1 to 3 on the growth of mouse tumors (S180 sarcoma) was evaluated. The results showed that the health-preserving pills provided in Examples 1 to 3 had a significant inhibitory effect on the growth of S180 tumors. When the dosage was 20 mg / kg, the tumor inhibition rates were 93.9% (Example 1), 82.4% (Example 2) and 86.8% (Example 3), respectively.

[0119] In summary, the present invention provides a health-preserving pill for improving immunity and preventing tumors. By selecting a variety of traditional Chinese medicinal materials and bioactive substances with immunomodulatory and anti-tumor effects, the pills act comprehensively on the body's immune system and tumor cells to jointly improve immunity and prevent tumors. The present invention aims to provide consumers with a safe, efficient and comprehensive health-care product, which has positive significance for improving body immunity, preventing tumors and promoting human health.

[0120] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A health-preserving pill for improving immunity and preventing tumors, characterized in that: The health-preserving pills include functional ingredients and auxiliary materials. In parts by weight, the functional ingredients include the following raw materials: 15-25 parts of chicken gizzard lining, 5-10 parts of codonopsis pilosula, 10-18 parts of astragalus, 3-7 parts of ginseng, 8-15 parts of mulberry linterus, 15-25 parts of loofah seeds, 30-40 parts of cucumber seeds, 18-30 parts of raw gardenia, and 7-12 parts of soft-shelled turtle protein peptide.

2. The health-preserving pill for improving immunity and preventing tumors according to claim 1, characterized in that: The functional ingredients include the following raw materials in parts by weight: 19 parts of chicken's gizzard lining, 8 parts of codonopsis pilosula, 14.5 parts of astragalus, 5 parts of ginseng, 10 parts of phellinus linteus, 20 parts of loofah seeds, 35 parts of cucumber seeds, 23 parts of raw gardenia, and 9.5 parts of soft-shelled turtle protein peptide.

3. The health-preserving pill for improving immunity and preventing tumors according to claim 1, characterized in that: The auxiliary material includes honey; the weight ratio of the honey to the effective ingredient is (0.9-1.2):(1-1.5).

4. A method for preparing the health-preserving pill for improving immunity and preventing tumors according to any one of claims 1 to 3, characterized in that: The preparation method comprises the following steps: Mix and crush Codonopsis pilosula, Astragalus membranaceus, Ginseng and Phellinus igniarius, and then perform supercritical extraction to obtain a first extract; Adding chicken gizzard lining, loofah seeds, cucumber seeds and raw gardenia to cyclohexane for reflux extraction to obtain a second extract; Mixing the first extract, the second extract and soft-shell turtle protein peptide to obtain the effective ingredient; The effective ingredients and auxiliary materials are mixed and then made into pills to obtain the health-preserving pills.

5. The method for preparing the health-preserving pills for improving immunity and preventing tumors according to claim 4, characterized in that: The working condition parameters of the supercritical extraction include: using a supercritical carbon dioxide fluid with a flow rate of 25 to 30 kg / h as a supercritical extraction medium, a supercritical extraction pressure of 55 to 60 MPa, a supercritical extraction temperature of 50 to 60° C., and a supercritical extraction time of 1 to 3 hours.

6. The method for preparing the health-preserving pills for improving immunity and preventing tumors according to claim 5, characterized in that: The supercritical extraction pressure is 57 MPa, and the supercritical extraction temperature is 56°C.

7. The method for preparing the health-preserving pills for improving immunity and preventing tumors according to claim 4, characterized in that: The working condition parameters of the reflux extraction include: the reflux extraction temperature is 85-95° C., the reflux extraction time is 1-3 hours, and the reflux extraction times are 1-3 times.

8. The method for preparing the health-preserving pills for improving immunity and preventing tumors according to claim 4, characterized in that: The added weight of the cyclohexane is (5-10) times the total weight of the chicken gizzard lining, loofah seeds, cucumber seeds and raw gardenia.

9. The method for preparing the health-preserving pills for improving immunity and preventing tumors according to claim 4, characterized in that: The pill making method is a molding method.