Preparation and method for increasing oil content of cyperus esculentus

Abstract

The invention discloses a preparation and a method for increasing the oil content of cyperus esculentus, and belongs to the technical field of agriculture. The preparation comprises the following raw materials in parts by weight: 3-5 parts of sesame leaf extract, 3-5 parts of seaweed extract, 1-3 parts of trace element chelate (chelate of magnesium, molybdenum, boron and tryptophan), 1-3 parts of salicylic acid and 80-100 parts of water, the components have a synergistic effect, and grease synthesis of cyperus esculentus can be effectively promoted, so that the oil content of cyperus esculentus seeds is remarkably increased, and the yield of cyperus esculentus is increased. The method has good economic value and application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of agriculture, and in particular to a preparation and a method for improving the oil content of jatropha. Background Art

[0002] Cyperus esculentus L. is an important economic crop with wide application value in the fields of food industry, pharmaceutical and chemical industry. It is rich in oil and is one of the ideal raw materials for producing edible oil and biodiesel. However, under natural growth conditions, the average oil content of Cyperus esculentus seeds usually fluctuates between 20-30%, which limits its development potential as a high-yield oil crop.

[0003] There are some methods for increasing the oil content of jatropha in the prior art, such as genetic modification, cultivation management and application of chemical regulators, and the respective situations are as follows:

[0004] Genetic improvement: There has been some research progress in gene editing of jatropha to increase its oil content through traditional breeding or modern molecular biology, but these methods are often time-consuming and costly.

[0005] Cultivation management: The yield and quality of jatropha can be indirectly affected by improving the planting environment such as soil fertility and water supply, but the operation is complicated and has limited effect on increasing the oil content in a single plant.

[0006] Chemical regulators: There are some products on the market that are intended to promote plant growth and development and may indirectly increase the content of certain components in the fruit, but effective solutions specifically for increasing the oil content of jatropha are still scarce.

[0007] In summary, although there have been many attempts to solve the problem of how to effectively increase the oil content of cyperus oleifera, the following major challenges are still faced: (1) The genetic improvement process is complex and time-consuming; (2) Simply relying on changing cultivation conditions is complex and difficult to achieve a significant increase in efficiency; (3) There is currently a lack of safe and efficient products on the market that can directly act on cyperus oleifera to enhance its oil content.

[0008] In view of the above situation, the present invention aims to provide a novel and effective preparation and method for increasing the oil content of cyperus oleifera, so as to overcome the shortcomings of the prior art and bring more significant economic benefits and social value to related industries. Summary of the invention

[0009] The purpose of the present invention is to provide a preparation and method for increasing the oil content of cyperus oleifera, so as to solve the problems existing in the above-mentioned prior art.

[0010] To achieve the above object, the present invention provides the following solutions:

[0011] One of the technical solutions of the present invention is a preparation for improving the oil content of cyperus oleifera, wherein the raw materials include the following components by weight: 3-5 parts of sesame leaf extract, 3-5 parts of seaweed extract, 1-3 parts of trace element chelate, 1-3 parts of salicylic acid and 80-100 parts of water; the trace element chelate is a chelate of magnesium, molybdenum, boron and tryptophan.

[0012] Both sesame leaf extract and seaweed extract are rich in a variety of nutrients and active substances required for the growth of cyperus oleifera. These substances can work synergistically to promote the expression of genes related to oil synthesis in cyperus oleifera cells, thereby increasing oil accumulation; certain components in sesame leaf extract and polysaccharides, amino acids and other substances in seaweed extract can also enhance the stress resistance of cyperus oleifera, such as drought resistance and disease resistance, and help cyperus oleifera maintain a high growth rate under adverse environmental conditions, thereby increasing its oil content; trace elements such as magnesium, molybdenum, and boron are indispensable nutrients for the growth and development of cyperus oleifera. After chelating with tryptophan, they can be better absorbed and utilized by cyperus oleifera and have a sustained-release effect, which helps to improve the nutritional status of cyperus oleifera and further Promote oil synthesis. In addition, tryptophan is the precursor of the synthesis of the endogenous hormone auxin indoleacetic acid. It can promote the synthesis of auxin, thereby affecting the physiological metabolic process of cyperus oleifera, especially fat metabolism, thereby increasing the oil content of cyperus oleifera. Salicylic acid can induce the expression of oil synthesis-related genes in cyperus oleifera cells, thereby increasing oil accumulation, and can also improve cyperus oleifera's ability to adapt to adversity, indirectly promoting oil accumulation, and salicylic acid can also promote cyperus oleifera cells to absorb and transport nutrients, thereby increasing the utilization rate of trace elements in cyperus oleifera. The synergistic combination of sesame leaf extract, seaweed extract, trace element chelates and salicylic acid can significantly increase the oil content of cyperus oleifera.

[0013] Furthermore, the preparation method of the sesame leaf extract comprises: crushing the sesame leaves, soaking and extracting with an ethanol solution, and drying the ethanol solution after the soaking and extraction to obtain the sesame leaf extract.

[0014] Furthermore, the concentration of the ethanol solution is 80-90 vol%; the mass volume ratio of the sesame leaves to the ethanol solution is 1 g:8-10 mL; the temperature of the immersion extraction is 65-70° C., and the time is 8-10 h.

[0015] The extract obtained from sesame leaves is rich in sesamin, which is a natural plant compound with a similar effect to plant hormones. It can affect various physiological processes in plants, enhance the overall growth and development of cyperus oleiferus plants, and thus increase the oil content of its seeds; sesamin has a strong antioxidant capacity, which can not only improve the stability of oils and fats, but also remove free radicals in plants and reduce the damage of oxidative stress to plants, which helps to protect the integrity of plant cell membranes, maintain normal metabolic activities, and promote fat synthesis and accumulation. In addition to sesamin, the extract obtained from sesame leaves may also contain other nutrients and active substances that can promote the growth and development of cyperus oleiferus. The present invention creatively finds that the extract obtained from sesame leaves can effectively increase the oil content of cyperus oleiferus, and the effect it can achieve is unattainable by other plant extracts.

[0016] Furthermore, the preparation method of the seaweed extract comprises: crushing the seaweed and mixing it with a mixed acid solution, soaking it for 4-6 hours and then ultrasonically treating it for 10-30 minutes; adding a mixed enzyme for enzymolysis after the ultrasonic treatment, inactivating the enzyme after the enzymolysis, cooling, centrifuging, and freeze-drying the centrifuge to obtain the seaweed extract.

[0017] Furthermore, the seaweed is kelp, ascophyllum nodosum or giant spirulina; the mixed acid is a mixed aqueous solution of acetic acid and citric acid; the power of the ultrasonic treatment is 200-250W; the mixed enzyme is a mixture of alginate lyase, cellulase and pectinase; the temperature of the enzymolysis is 30-35°C and the time is 2-4h.

[0018] Furthermore, the soaking is carried out at room temperature.

[0019] Furthermore, the concentration of acetic acid in the mixed acid is 0.5-1 mol / L, and the concentration of citric acid is 0.3-0.5 mol / L; the mass volume ratio of the seaweed to the mixed acid is 1 g:10-12 mL.

[0020] Furthermore, the mass ratio of alginate lyase, cellulase and pectinase in the mixed enzyme is 3-5:2-4:1-2; and the added amount of the mixed enzyme is 1-2wt% of the seaweed.

[0021] Furthermore, before adding the mixed enzyme for enzymolysis, the process also includes adjusting the pH of the mixed system after ultrasonic treatment to 7.0-7.5.

[0022] Furthermore, the temperature of the enzyme inactivation treatment is 75-85°C and the time is 2-4h.

[0023] The extract obtained from seaweed is rich in seaweed polysaccharides, highly unsaturated fatty acids and plant hormones. Seaweed polysaccharides can promote the growth of Cyperus oleifera and enhance its disease resistance, thereby contributing to the accumulation of oil in Cyperus oleifera. Highly unsaturated fatty acids have multiple physiological functions in Cyperus oleifera and can promote its normal growth and metabolism, thereby affecting its oil synthesis and accumulation. Plant hormones can regulate the growth and development process of Cyperus oleifera and promote the synthesis and accumulation of oil. The synergistic effect of these ingredients contained in the extract obtained from seaweed can effectively increase the oil content of Cyperus oleifera.

[0024] Furthermore, the preparation method of the trace element chelate includes: mixing tryptophan, magnesium sulfate, ammonium molybdate, borax and water, adjusting the pH to 7.0-7.5, and performing a first stage chelation reaction; after the first stage chelation reaction is completed, adjusting the pH to 6.0-6.5, and performing a second stage chelation reaction; after the second stage chelation reaction is completed, concentrating and drying to obtain the trace element chelate.

[0025] Furthermore, the mass ratio of magnesium sulfate, ammonium molybdate and borax is 3-5:5-8:2-3, and the mass ratio of the sum of the masses of magnesium sulfate, ammonium molybdate and borax to the mass of tryptophan is 1:3-5; the temperature of the first stage chelation reaction is 30-35°C, and the time is 30-40min; the temperature of the second stage chelation reaction is 60-70°C, and the time is 20-30min.

[0026] Furthermore, the ratio of the sum of the masses of tryptophan, magnesium sulfate, ammonium molybdate and borax to the mass of water is 1:90-100.

[0027] The second technical solution of the present invention is a method for preparing the above-mentioned preparation for improving the oil content of cyperus oleifera, comprising the following steps: mixing sesame leaf extract, seaweed extract, trace element chelate, salicylic acid and water, and stirring evenly to obtain the preparation for improving the oil content of cyperus oleifera.

[0028] The third technical solution of the present invention is a method for increasing the oil content of cyperus oleifera, wherein the preparation for increasing the oil content of cyperus oleifera is applied once every 5-7 days by foliar spraying 40 days after the emergence of cyperus oleifera, the dosage of each application is 1-2 L / mu, and a total of 3-5 applications are performed.

[0029] Furthermore, before the foliar spraying, the preparation for increasing the oil content of jatropha is diluted 15-20 times with water.

[0030] The present invention discloses the following technical effects:

[0031] The invention provides a preparation for improving the oil content of cyperus oleifera. The raw materials include 3-5 parts of sesame leaf extract, 3-5 parts of seaweed extract, 1-3 parts of trace element chelate (chelate of magnesium, molybdenum, boron and tryptophan), 1-3 parts of salicylic acid and 80-100 parts of water. The components act synergistically to effectively promote the oil synthesis of cyperus oleifera, thereby significantly improving the oil content of its seeds. The preparation has good economic value and application prospect.

[0032] All ingredients in the preparation of the present invention are of natural origin or safe chemical substances, are environmentally friendly, will not cause pollution to soil and water sources, meet the requirements of sustainable development of modern agriculture, and are conducive to the development of ecological agriculture.

[0033] The preparation method of the preparation of the invention is simple, the raw materials are readily available, and the production cost is relatively low. In addition, the preparation is applied by foliar spraying, and no complicated cultivation and management measures are required. The operation is simple and easy, and the preparation is suitable for promotion and use in rural areas. DETAILED DESCRIPTION

[0034] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0035] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0036] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0037] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present application description and examples are exemplary only.

[0038] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0039] The room temperature involved in the following examples and comparative examples specifically refers to 20-30°C.

[0040] The raw materials and reagents used in the following examples and comparative examples are all common commercially available products, wherein the enzyme activities of alginate lyase, cellulase and pectinase are all 200,000 U / g.

[0041] Example 1

[0042] A preparation for improving the oil content of cyperus oleifera, comprising the following raw materials by weight: 3 parts of sesame leaf extract, 3 parts of seaweed extract, 1 part of trace element chelate (chelate of magnesium, molybdenum, boron and tryptophan), 1 part of salicylic acid and 80 parts of water.

[0043] The preparation method of the above-mentioned preparation for improving the oil content of cyperus oleifera is as follows: sesame leaf extract, seaweed extract, trace element chelate, salicylic acid and water are mixed and stirred evenly to obtain the preparation for improving the oil content of cyperus oleifera.

[0044] The preparation method of sesame leaf extract is as follows: the dried sesame leaves are crushed through a 40-mesh sieve, 80 vol% ethanol solution is added at a mass volume ratio of 1 g:8 mL, and the sesame leaf extract is soaked and extracted at 65° C. for 8 hours. After the soaking and extraction, the ethanol solution is dried by a rotary evaporator to obtain the sesame leaf extract.

[0045] The preparation method of seaweed extract is as follows: dried kelp is crushed and passed through a 40-mesh sieve, and then mixed with a mixed acid solution (a mixed aqueous solution of acetic acid and citric acid, wherein the concentration of acetic acid is 0.5 mol / L and the concentration of citric acid is 0.3 mol / L) at a mass volume ratio of 1 g:10 mL, and then soaked at room temperature for 4 hours and then ultrasonically treated for 10 minutes at an ultrasonic power of 200 W; after the ultrasonic treatment, the pH of the mixed system after the ultrasonic treatment is adjusted to 7.0, and a mixed enzyme (alginate lyase, cellulase and pectinase are mixed in a mass ratio of 3:2:1, and the added amount of the mixed enzyme is 1wt% of the seaweed) is added to perform enzymolysis at a temperature of 30°C for 2 hours, and after the enzymolysis is completed, the enzyme is inactivated at 75°C for 2 hours, cooled, centrifuged, and the centrifuge is freeze-dried (the freeze-drying temperature is -30°C) to obtain the seaweed extract.

[0046] The preparation method of the trace element chelate is as follows: tryptophan, magnesium sulfate, ammonium molybdate, borax and water are mixed (the mass ratio of magnesium sulfate, ammonium molybdate and borax is 3:5:2, the mass ratio of the sum of the mass of magnesium sulfate, ammonium molybdate and borax to the mass of tryptophan is 1:3, and the mass ratio of the sum of the mass of tryptophan, magnesium sulfate, ammonium molybdate and borax to water is 1:90), the pH is adjusted to 7.0, and a first-stage chelation reaction is carried out at a temperature of 30°C for 30 minutes; after the first-stage chelation reaction is completed, the pH is adjusted to 6.0, and a second-stage chelation reaction is carried out at a temperature of 60°C for 20 minutes; after the second-stage chelation reaction is completed, the mixture is concentrated and dried at 90°C to obtain the trace element chelate.

[0047] Example 2

[0048] A preparation for improving the oil content of cyperus oleifera, comprising the following raw materials by weight: 4 parts of sesame leaf extract, 4 parts of seaweed extract, 2 parts of trace element chelate (chelate of magnesium, molybdenum, boron and tryptophan), 2 parts of salicylic acid and 90 parts of water.

[0049] The preparation method of the above-mentioned preparation for improving the oil content of cyperus oleifera is as follows: sesame leaf extract, seaweed extract, trace element chelate, salicylic acid and water are mixed and stirred evenly to obtain the preparation for improving the oil content of cyperus oleifera.

[0050] The preparation method of sesame leaf extract is as follows: the dried sesame leaves are crushed through a 40-mesh sieve, 85 vol% ethanol solution is added at a mass volume ratio of 1 g:9 mL, and the mixture is soaked and extracted at 68° C. for 9 hours. After the soaking and extraction, the ethanol solution is dried by a rotary evaporator to obtain the sesame leaf extract.

[0051] The preparation method of seaweed extract is as follows: the dried kelp is crushed and passed through a 40-mesh sieve, and then mixed with a mixed acid solution (a mixed aqueous solution of acetic acid and citric acid, wherein the concentration of acetic acid is 0.8 mol / L and the concentration of citric acid is 0.4 mol / L) at a mass volume ratio of 1 g:11 mL, and then soaked at room temperature for 5 hours and then ultrasonically treated for 20 minutes at an ultrasonic power of 220 W; after the ultrasonic treatment, the pH of the mixed system after the ultrasonic treatment is adjusted to 7.2, and a mixed enzyme (alginate lyase, cellulase and pectinase are mixed in a mass ratio of 4:3:2, and the added amount of the mixed enzyme is 1.5 wt% of the seaweed) is added to perform enzymolysis at a temperature of 32° C. for 3 hours, and after the enzymolysis is completed, the enzyme is inactivated at 80° C. for 3 hours, cooled, centrifuged, and the centrifuge is freeze-dried (the freeze-drying temperature is -30° C.) to obtain the seaweed extract.

[0052] The preparation method of the trace element chelate is as follows: tryptophan, magnesium sulfate, ammonium molybdate, borax and water are mixed (the mass ratio of magnesium sulfate, ammonium molybdate and borax is 4:6:3, the mass ratio of the sum of the mass of magnesium sulfate, ammonium molybdate and borax to the mass of tryptophan is 1:4, and the mass ratio of the sum of the mass of tryptophan, magnesium sulfate, ammonium molybdate and borax to water is 1:95), the pH is adjusted to 7.2, and a first-stage chelation reaction is carried out at a temperature of 32°C for 35 minutes; after the first-stage chelation reaction is completed, the pH is adjusted to 6.2, and a second-stage chelation reaction is carried out at a temperature of 65°C for 25 minutes; after the second-stage chelation reaction is completed, the mixture is concentrated and dried at 90°C to obtain the trace element chelate.

[0053] Example 3

[0054] A preparation for improving the oil content of cyperus oleifera, comprising the following raw materials by weight: 5 parts of sesame leaf extract, 5 parts of seaweed extract, 3 parts of trace element chelate (chelate of magnesium, molybdenum, boron and tryptophan), 3 parts of salicylic acid and 100 parts of water.

[0055] The preparation method of the above-mentioned preparation for improving the oil content of cyperus oleifera is as follows: sesame leaf extract, seaweed extract, trace element chelate, salicylic acid and water are mixed and stirred evenly to obtain the preparation for improving the oil content of cyperus oleifera.

[0056] The preparation method of sesame leaf extract is as follows: the dried sesame leaves are crushed through a 40-mesh sieve, a 90 vol% ethanol solution is added at a mass volume ratio of 1 g:10 mL, and the sesame leaf extract is soaked and extracted at 70° C. for 10 h. After the soaking and extraction, the ethanol solution is dried by a rotary evaporator to obtain the sesame leaf extract.

[0057] The preparation method of seaweed extract is as follows: the dried kelp is crushed and passed through a 40-mesh sieve, and then mixed with a mixed acid solution (a mixed aqueous solution of acetic acid and citric acid, wherein the concentration of acetic acid is 1 mol / L and the concentration of citric acid is 0.5 mol / L) at a mass volume ratio of 1 g:12 mL, and then soaked at room temperature for 6 hours and then ultrasonically treated for 30 minutes at an ultrasonic power of 250 W; after the ultrasonic treatment, the pH of the mixed system after the ultrasonic treatment is adjusted to 7.5, and a mixed enzyme (alginate lyase, cellulase and pectinase are mixed in a mass ratio of 5:4:2, and the added amount of the mixed enzyme is 2 wt% of the seaweed) is added to perform enzymolysis at a temperature of 35°C for 4 hours, and after the enzymolysis is completed, the enzyme is inactivated at 85°C for 4 hours, cooled, centrifuged, and the centrifuge is freeze-dried (the freeze-drying temperature is -30°C) to obtain the seaweed extract.

[0058] The preparation method of the trace element chelate is as follows: tryptophan, magnesium sulfate, ammonium molybdate, borax and water are mixed (the mass ratio of magnesium sulfate, ammonium molybdate and borax is 5:8:3, the mass ratio of the sum of the mass of magnesium sulfate, ammonium molybdate and borax to the mass of tryptophan is 1:5, and the mass ratio of the sum of the mass of tryptophan, magnesium sulfate, ammonium molybdate and borax to water is 1:100), the pH is adjusted to 7.5, and a first-stage chelation reaction is carried out at a temperature of 35°C for 40 minutes; after the first-stage chelation reaction is completed, the pH is adjusted to 6.5, and a second-stage chelation reaction is carried out at a temperature of 70°C for 30 minutes; after the second-stage chelation reaction is completed, the mixture is concentrated and dried at 90°C to obtain the trace element chelate.

[0059] Comparative Example 1

[0060] Same as Example 1, except that the sesame leaf extract is replaced by an equal mass of peanut leaf extract.

[0061] The preparation method of peanut leaf extract is as follows: the dried peanut leaves are crushed through a 40-mesh sieve, 80 vol% ethanol solution is added at a mass volume ratio of 1 g:8 mL, and the peanut leaf extract is soaked at 65° C. for 8 h. After the soaking and extraction, the ethanol solution is dried by a rotary evaporator to obtain the peanut leaf extract.

[0062] Comparative Example 2

[0063] Same as Example 1, except that the seaweed extract is replaced with seaweed polysaccharide of equal mass.

[0064] Comparative Example 3

[0065] The same as Example 1, except that the trace element chelate used is a chelate of zinc, molybdenum, boron and tryptophan. Specifically, in the preparation process of the trace element chelate, magnesium sulfate is replaced by zinc sulfate of the same mass, and other preparation conditions remain unchanged.

[0066] Comparative Example 4

[0067] The same as Example 1, except that the trace element chelate used is a chelate of magnesium, molybdenum, boron and glutamic acid. Specifically, in the preparation process of the trace element chelate, tryptophan is replaced by an equal mass of glutamic acid, and other preparation conditions remain unchanged.

[0068] Comparative Example 5

[0069] Same as Example 1, except that salicylic acid is replaced by jasmonic acid of equal mass.

[0070] Application Example 1

[0071] Application test of the preparation for improving the oil content of Cyperus oleifera in Cyperus oleifera planting

[0072] Experimental cyperus rotundus variety: The cyperus rotundus variety tested was Zhongyousha No. 3.

[0073] Test location: The test field is located in the planting area of ​​Cyperus oleifera test field in Maoming City, Guangdong Province.

[0074] Experimental design: 27 experimental plots (3 plots in a group, a total of 9 experimental groups) were randomly divided in the cyperus oleifera test field with uniform soil properties and medium fertility. Each experimental plot was 50m2 in area. 2 . The seeds of cyperus oleifera were first exposed to the sun for 2 days, then stored for one week and soaked in warm water (water temperature of about 45°C) for 1 day for germination. After germination, they were sown in each test plot, with a seed rate of 8 kg per mu, a sowing depth of about 5 cm, 9,000 holes per mu, 2 seeds per hole, a row spacing of 30 cm, a plant spacing of 15 cm, and fertilizer and water management according to local field production requirements. 40 days after the emergence of cyperus oleifera, the preparations for improving the oil content of cyperus oleifera prepared in Examples 1-3, the preparations for improving the oil content of cyperus oleifera prepared in Comparative Examples 1-5 (the application dosage was 1.5 L / mu, diluted 20 times with water and sprayed on the leaves) and water (control group) were applied to the 9 test groups respectively, once every 6 days, for a total of 4 times.

[0075] Oil content determination: At maturity, 10 plants were randomly selected from each plot of each test group, and pulled up by hand (about 70% of the fruits were under the stump) or dug up with a rake. After the fruits were exposed to the sun for 2 days with the fruits facing upward, they were picked by hand or beaten with a short wooden stick and sieved with a sieve. After drying, the oil content in the seeds of Cyperus oleifera was determined using the method published in the agricultural industry standard (NY / T 1285-2007). The average value of the three test plots in each test group was taken as the final result, and the final determination results are shown in Table 1.

[0076] Table 1

[0077] Grouping Oil content / % Example 1 32.9 Example 2 33.8 Example 3 32.4 Comparative Example 1 27.9 Comparative Example 2 29.2 Comparative Example 3 31.0 Comparative Example 4 30.1 Comparative Example 5 28.6 Control group 22.9

[0078] From the results in Table 1, it can be seen that the oil content of the cyperus oleifera treated with the preparations for increasing the oil content of cyperus oleifera prepared in Examples 1-3 of the present invention is significantly increased, which is significantly better than other comparative examples and the control group. This shows that the technical effect of significantly increasing the oil content of cyperus oleifera by the preparations for increasing the oil content of the present invention is the result of the synergistic effect of the various components.

[0079] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A preparation for increasing the oil content of Cyperus oleifera, characterized in that: The raw materials include the following components by mass: 3-5 parts of sesame leaf extract, 3-5 parts of seaweed extract, 1-3 parts of trace element chelate, 1-3 parts of salicylic acid and 80-100 parts of water; the trace element chelate is a chelate of magnesium, molybdenum, boron and tryptophan.

2. The preparation for increasing the oil content of Cyperus oleifera according to claim 1, characterized in that The preparation method of the sesame leaf extract comprises the following steps: crushing the sesame leaves, soaking and extracting the sesame leaves with an ethanol solution, and drying the ethanol solution after the soaking and extraction to obtain the sesame leaf extract.

3. The preparation for increasing the oil content of Cyperus oleifera according to claim 2, characterized in that: The concentration of the ethanol solution is 80-90 vol%; the mass volume ratio of the sesame leaves to the ethanol solution is 1 g:8-10 mL; the temperature of the soaking extraction is 65-70° C., and the time is 8-10 h.

4. The preparation for increasing the oil content of Cyperus oleifera according to claim 1, characterized in that: The preparation method of the seaweed extract comprises: crushing the seaweed and mixing it with a mixed acid solution, soaking it for 4-6 hours and then ultrasonically treating it for 10-30 minutes; adding a mixed enzyme for enzymolysis after the ultrasonic treatment, inactivating the enzyme after the enzymolysis, cooling, centrifuging, and freeze-drying the centrifuge to obtain the seaweed extract.

5. The preparation for increasing the oil content of Cyperus oleifera according to claim 4, characterized in that: The seaweed is kelp, ascophyllum nodosum or giant spirulina; the mixed acid is a mixed aqueous solution of acetic acid and citric acid; the power of the ultrasonic treatment is 200-250W; the mixed enzyme is a mixture of alginate lyase, cellulase and pectinase; the temperature of the enzymolysis is 30-35°C and the time is 2-4h.

6. The preparation for increasing the oil content of Cyperus oleifera according to claim 4, characterized in that: The method also includes adjusting the pH of the mixed system after ultrasonic treatment to 7.0-7.5 before adding the mixed enzyme for enzymolysis.

7. The preparation for increasing the oil content of Cyperus oleifera according to claim 1, characterized in that: The preparation method of the trace element chelate comprises: mixing tryptophan, magnesium sulfate, ammonium molybdate, borax and water, adjusting the pH to 7.0-7.5, and performing a first-stage chelation reaction; after the first-stage chelation reaction is completed, adjusting the pH to 6.0-6.5, and performing a second-stage chelation reaction; after the second-stage chelation reaction is completed, concentrating and drying to obtain the trace element chelate.

8. The preparation for increasing the oil content of Cyperus oleifera according to claim 7, characterized in that: The mass ratio of magnesium sulfate, ammonium molybdate and borax is 3-5:5-8:2-3, and the mass ratio of the sum of the mass of magnesium sulfate, ammonium molybdate and borax to the mass of tryptophan is 1:3-5; the temperature of the first stage chelation reaction is 30-35°C, and the time is 30-40min; the temperature of the second stage chelation reaction is 60-70°C, and the time is 20-30min.

9. A method for preparing a preparation for improving the oil content of Cyperus oleifera according to any one of claims 1 to 8, characterized in that: The following steps are involved: Sesame leaf extract, seaweed extract, trace element chelate, salicylic acid and water are mixed and stirred evenly to obtain the preparation for improving the oil content of Cyperus oleifera.

10. A method for increasing the oil content of jatropha, characterized in that: 40 days after the emergence of cyperus oleifera, the preparation for improving the oil content of cyperus oleifera according to any one of claims 1 to 8 is applied once by foliar spraying every 5 to 7 days, with a dosage of 1 to 2 L / mu each time, and applied 3 to 5 times in total.