Exfoliated cell component extraction method based on exfoliated cell visual discovery and extraction instrument

Abstract

The invention discloses an exfoliated cell component extraction method based on an exfoliated cell visual discovery and extraction instrument. The method aims at extraction of residual exfoliated cell components hidden in textile fabrics, extraction of residual exfoliated cell components of old detection materials with firm surface combination or detection materials with severe surface weathering, extraction of residual exfoliated cell components of detection materials existing in gullies, and extraction of residual exfoliated cell components with tight limited combination on the surfaces of partial textile fabrics. Extracting residual exfoliated cell components of the rust-containing test material and extracting residual exfoliated cell components of the test material contacted by multiple persons; according to the invention, a display is used for knowing the cause, spatial structure relationship and combination degree between exfoliated cell residues and a detected material body under the intuitive condition of a high-definition and high-magnification real-time visualization technology, cognitive preparation is made for effective extraction in the next step, extraction is carried out by means of a tool, cell components are extracted and analyzed by adopting high-speed centrifugation and membrane filtration, and the detection result is accurate. The extraction process is novel and the extraction effect is remarkable.

Description

Technical Field

[0001] The invention relates to the technical field of extracting exfoliated cell component substances, and in particular to an exfoliated cell component extraction method based on an exfoliated cell visualization discovery extractor. Background Art

[0002] At present, the extraction of exfoliated cell components generally adopts the method of wiping with cotton swabs or direct collection with adhesive devices. There is no effective and successful extraction method for exfoliated cells and other components that exist in some complex samples or are firmly attached to the surface of the samples.

[0003] See also Figure 1 As shown, for some complex evidence and some old evidence, the remaining exfoliated cell components cannot be effectively extracted, and subsequent PCR cannot obtain effective typing results. Figure 1 A schematic diagram of the prior art for extracting a large amount of exfoliated cell components from an old wooden knife handle is shown. Since the remaining exfoliated cells, mixed with oil and other components, are firmly bonded to the knife handle, and due to years of weathering and drying, a solid membrane shell similar to a protective film has formed on the surface. The usual cotton swab wiping and adhesive extraction methods are no longer able to effectively break the shell and extract the exfoliated cell components under the protective film. Conventional extraction methods have been used on this physical evidence many times, but no effective exfoliated cell components and PCR typing have been obtained.

[0004] The conventional method of using a cotton swab is to dip the cotton swab in saline and vigorously wipe the surface of the sample. However, since such samples have been weathered and dried for many years, the exfoliated cell components and mixed oils remaining on the surface have formed a relatively strong protective film on the surface, and have been left for a long time. The exfoliated cell components on the surface of this protective film have been degraded and lost their activity. Since this protective film isolates the active exfoliated cell components inside from the outside world, the conventional cotton swab wiping method cannot destroy this protective film, and therefore, it is impossible to effectively extract the active exfoliated cell components inside. The adhesive extraction method is even more ineffective for such samples because it can only extract components that are not firmly attached to the surface.

[0005] Therefore, based on the above technical problems, technicians in this field urgently need to develop a method for extracting exfoliated cell components based on an exfoliated cell visualization discovery extractor. Summary of the invention

[0006] The purpose of the present invention is to provide a method for extracting exfoliated cell components based on an exfoliated cell visualization discovery extractor, which uses a display to intuitively understand the causes, spatial structural relationships, and degree of combination between the exfoliated cell residues and the sample body under high-definition, high-magnification, real-time visualization technology, so as to prepare for the next step of effective extraction. The extraction is carried out with the help of designated tools, and then high-speed centrifugation + membrane filtration is used to extract and analyze the cell components. The overall extraction process is novel and the extraction effect is significant.

[0007] In order to achieve the above object, the present invention provides the following technical solutions: The exfoliated cell component extraction method based on the exfoliated cell visualization discovery extractor of the present invention is used to extract residual exfoliated cell components embedded in textiles, residual exfoliated cell components of old samples with strong surface bonding or samples with severe surface weathering, residual exfoliated cell components of samples existing in gullies, residual exfoliated cell components of some textile surfaces and internal fibers that are tightly bonded, residual exfoliated cell components of samples containing rust, and residual exfoliated cell components of samples that have been touched by multiple people; The method for extracting exfoliated cell components is aimed at extracting old samples with strong surface bonding or samples with severe surface weathering by using a stripping tool for stripping and extraction; The exfoliated cell component extraction method is used for extracting residual exfoliated cell components from a sample containing rust, and separates iron ions or ferric oxide by a high-speed centrifuge and membrane filtration.

[0008] Furthermore, the stripping tool is a knife.

[0009] Further, the stripping tool comprises: A handle, a knife bar and a blade fixed to the front end of the knife bar; The stripping tool uses the blade to strip off the detached cell components on the surface of old specimens that are firmly bonded to the surface or specimens that are severely weathered; The peeled off cell component material is then observed by an exfoliated cell visualization extraction instrument and displayed on a display.

[0010] Furthermore, when extracting the residual exfoliated cell components from the sample containing rust, the specific extraction steps include: S1. Use a high-speed centrifuge to centrifuge rust, iron ions or larger impurities to the bottom or separate them into other layers; S2. After centrifugation, a suspension liquid mixture of relatively clean cells and cell fragments and a certain amount of iron ions in the corresponding part is drawn; S3. Use a filter to repeatedly filter and rinse the suspension to separate cell components and iron ions or metal impurities.

[0011] Furthermore, the filter includes: filter bowl; and a funnel connected to the lower end of the filter cup; The filter cup and the funnel are connected by a snap connection or a threaded connection; Filter paper is arranged at the connection between the filter cup and the funnel and inside the filter cup.

[0012] Furthermore, the pore size of the filter paper is 500nm~1000nm.

[0013] In the above technical scheme, the present invention provides a method for extracting exfoliated cell components based on an exfoliated cell visualization discovery extractor, which has the following beneficial effects: The extraction method of the present invention utilizes a display to intuitively understand the causes, spatial structural relationships, and degree of binding between shed cell residues and the sample body under high-definition, high-magnification, real-time visualization technology, so as to prepare for the next step of effective extraction. The extraction is performed with the aid of designated tools, and then high-speed centrifugation + membrane filtration is used to extract and analyze cell components. The overall extraction process is novel and the extraction effect is significant. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. It is obvious that the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0015] Figure 1 The effect diagram of conventional extraction method identification in the prior art; Figure 2 It is a system flow chart of the method for extracting exfoliated cell components based on the exfoliated cell visualization discovery extractor disclosed in an embodiment of the present invention; Figure 3 A schematic diagram of an exfoliated cell visualization discovery extractor for an exfoliated cell component extraction method based on an exfoliated cell visualization discovery extractor disclosed in an embodiment of the present invention; Figure 4 It is a schematic diagram of the structure of a stripping tool for a method for extracting exfoliated cell components based on an exfoliated cell visualization discovery extractor disclosed in an embodiment of the present invention; Figure 5 It is a schematic diagram of the structure of a filter of an exfoliated cell component extraction method based on an exfoliated cell visualization discovery extractor disclosed in an embodiment of the present invention.

[0016] Description of reference numerals: 1. Exfoliated cell detector; 2. Operating handle; 3. HP all-in-one machine; 4. Peeling tool; 5. Beaker; 6. Funnel; 7. Filter paper; 401. handle; 402. knife bar; 403. blade. DETAILED DESCRIPTION

[0017] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0018] See also Figures 2 to 5 As shown; The present embodiment provides a method for extracting exfoliated cell components based on an exfoliated cell visualization discovery extractor. The method is used to extract residual exfoliated cell components embedded in textiles, residual exfoliated cell components of old samples with strong surface bonding or samples with severe surface weathering, residual exfoliated cell components of samples existing in gullies, residual exfoliated cell components of samples with tight surface bonding on some textile surfaces, residual exfoliated cell components of samples containing rust, and residual exfoliated cell components of samples that have been touched by multiple people. The method for extracting exfoliated cell components is aimed at extracting old samples with strong surface bonding or samples with severe surface weathering by using a stripping tool 4 for stripping and extraction; The exfoliated cell component extraction method is used for extracting residual exfoliated cell components from a sample containing rust, and separates iron ions or ferric oxide by a high-speed centrifuge and membrane filtration.

[0019] Specifically, the method for extracting exfoliated cell components disclosed in this embodiment is specifically divided into: 1. Hidden in textiles: The residual exfoliated cell components are mostly hidden inside the complex fibers of textiles, and conventional cotton swab wiping and adhesive collection methods are ineffective. The extraction method for this type of sample is to use scissors-like tools to directly cut the exfoliated cells directly from the exfoliated cell aggregation area after seeing the causal relationship on the visual display, or even cut the textile into pieces to effectively release the residual exfoliated cell components inside, so as to obtain effective exfoliated cell components and prepare the necessary and sufficient conditions for the next PCR.

[0020] 2. Old samples that are firmly bonded to the surface or samples with severely weathered surfaces: The surfaces of such samples that contain exfoliated cell components usually have formed a firm protective film / shell, and conventional cotton swab wiping and adhesive extraction are ineffective; and if they are directly cut off together with the sample material itself, the protective film on the surface and the upper and lower layers of the sample material itself will still form a wrapping structure similar to the residual exfoliated cell components inside, and the internal exfoliated cell components cannot be effectively released, resulting in ineffective extraction. The extraction method for this type of sample is to use a blade to scrape layer by layer to destroy the surface protective film after seeing the cause and spatial structure relationship on a visual display in real time, and then scrape off the exfoliated cell components inside layer by layer, and effectively collect them. When a certain amount is reached, it can effectively prepare the necessary and sufficient conditions for the next PCR step.

[0021] 3. Samples in gullies: Some samples have structures similar to gullies or incomplete structures. Such structures are prone to accumulation of residual exfoliated cell components, and conventional cotton swab wiping and adhesive collection methods cannot effectively collect them, resulting in ineffective extraction. The extraction method for this type of sample is to use a sharp knife tip or a hook hook or other sharp tool to effectively extract the residual exfoliated cells in the gullies or incomplete structures after seeing the cause and spatial structure relationship in real time on a visual display. After collecting a certain amount, it can effectively prepare the necessary and sufficient conditions for the next PCR.

[0022] 4. Exfoliated cell clusters that are tightly bound to the fibers on the surface of some textiles: This type of sample is characterized by a firm bond between the exfoliated cell clusters and the fibers of the textiles. Conventional cotton swab wiping and adhesive extraction are ineffective. This type of sample is also not suitable for methods such as blade scraping, which often results in ineffective extraction. The extraction method for this type of sample is to use special tweezers to accurately clamp the cell-containing substances or cell clusters directly under intuitive observation under the display of high-definition, high-magnification real-time visualization technology, so as to obtain effective extraction and prepare the necessary and sufficient conditions for the next step of PCR.

[0023] 5. Extraction of exfoliated cells from samples containing rust: Since such samples are mostly iron metal tools or instruments, a large amount of rust or iron ion components will form on the iron surface over time. After these components are mixed with the exfoliated cells, it is difficult to completely separate the iron ions and other metal components from the exfoliated cell DNA components during the subsequent conventional centrifugation separation. As a result, a large amount of residual iron ions will interfere with or inhibit the subsequent PCR process during the next step of magnetic bead or silica bead extraction, resulting in typing failure.

[0024] The extraction method of this type of sample is: High-speed centrifugation (already included in the standard process) + membrane filtration extraction method (new invention added) Note: Currently, the only conventional separation method is centrifugation. This invention adds "membrane filtration extraction method" on the basis of high-speed centrifugation.

[0025] The membrane filter is a specially customized filter or filter mesh with a pore size of about 500-1000 nanometers (pore sizes of different sizes can be used as needed).

[0026] 6. Extraction of samples that have been touched by multiple people: Since such samples have been touched by multiple people, there will be residual exfoliated cell DNA from multiple people. If they are directly extracted blindly, mixed extraction will result, and effective typing cannot be achieved later. The extraction method for such samples is to extract each site containing exfoliated cell components separately under the conditions of high-definition and high-magnification real-time visualization technology, so that relatively independent personal exfoliated cell components can be extracted, and a single effective typing can be obtained later.

[0027] See also Figure 4 As shown, the stripping tool of this embodiment is a cutting tool. Specifically, the stripping tool 4 of this embodiment includes: A handle 401, a knife bar 402, and a blade 403 fixed to the front end of the knife bar 402; Among them, the stripping tool 4 of this embodiment uses the blade 403 to strip the exfoliated cell components on the surface of old samples with firm surface bonding or samples with serious surface weathering; the exfoliated cell components are then observed by the exfoliated cell visualization discovery extractor and displayed on the display.

[0028] Preferably, when extracting the residual exfoliated cell components from the sample containing rust in this embodiment, the specific extraction steps include: S1. Use a high-speed centrifuge to centrifuge rust, iron ions or larger impurities to the bottom or separate them into other layers; S2. After centrifugation, a suspension liquid mixture of relatively clean cells and cell fragments and a certain amount of iron ions in the corresponding part is drawn; S3. Use a filter to repeatedly filter and rinse the suspension to separate cell components and iron ions or metal impurities.

[0029] The filter of this embodiment includes a filter cup 5 and a funnel 6 connected to the lower end of the filter cup 5; The filter cup 5 and the funnel 6 are connected by snap-fit ​​or threaded connection; a filter paper 7 is provided at the connection between the filter cup 5 and the funnel 6 and inside the filter cup 5. The pore size of the filter paper 7 in this embodiment is 500nm~1000nm. As a preferred embodiment, the pore size of the filter paper 7 in this embodiment can also be 1000-2000nm or 2000-5000nm, and different membrane filters are used according to different substances to be separated.

[0030] When extracting exfoliated cells containing impurities such as rust and iron ions, after realizing that there are a large number of rust or iron ion components mixed, conventional high-speed centrifugation is first used to centrifuge most of the rust or iron ions or larger impurities to the bottom or separate them to other layers, and the relatively clean cells and cell fragments in the corresponding part and the suspension liquid mixture with a certain amount of iron ions are sucked for the next step; then, the above suspension is repeatedly filtered and rinsed using a customized filter with a pore size of about 500-1000 nanometers; since the molecular diameter of iron ions or ferric oxide is about 20-40 nanometers or smaller, it can be effectively removed under this filter. Cells or cell fragments are generally larger than 1 micron and can be intercepted and retained by the filter, thereby effectively separated. This method can effectively separate components such as iron ions or metal impurities, and prepare sufficient and necessary conditions for later PCR typing.

[0031] The exfoliated cell visualization discovery and extraction instrument involved in this embodiment is an instrument previously applied for and protected by the applicant. It has high-definition and high-magnification real-time visualization display technology: 4K magnification is greater than 500 times, with clear display, and is used to directly observe the spatial structural attachment relationship between the exfoliated cell components and the sample in real time.

[0032] Meanwhile, the stripping tool involved in this embodiment is not limited to the stripping tool 4, and is specifically divided into: Various scrapers or surgical blades, etc.: used for the exfoliated cell components that are densely combined with the specimen, and a firm film has formed on the surface. The blades can be used to scrape the capsule and effectively separate and extract the residual components inside.

[0033] Various tweezers, hooks, scrapers, etc.: used to clamp, pick, hook, scrape, etc. the exfoliated cell components present in grooves or defects on the surface.

[0034] Various scissors and clamps: used to cut out the exfoliated cells inside some textiles or those firmly attached to the surface, etc., and can be used for cutting, clamping and crushing.

[0035] In the above technical scheme, the present invention provides a method for extracting exfoliated cell components based on an exfoliated cell visualization discovery extractor, which has the following beneficial effects: The extraction method of the present invention utilizes a display to intuitively understand the causes, spatial structural relationships, and degree of binding between shed cell residues and the sample body under high-definition, high-magnification, real-time visualization technology, so as to prepare for the next step of effective extraction. The extraction is performed with the aid of designated tools, and then high-speed centrifugation + membrane filtration is used to extract and analyze cell components. The overall extraction process is novel and the extraction effect is significant.

[0036] The above description is only by way of illustration of certain exemplary embodiments of the present invention. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A method for extracting exfoliated cell components based on an exfoliated cell visualization extraction instrument, characterized in that: The exfoliated cell component extraction method is used to extract residual exfoliated cell components embedded in textiles, residual exfoliated cell components of old samples with strong surface bonding or samples with severe surface weathering, residual exfoliated cell components of samples existing in gullies, residual exfoliated cell components of samples with tight surface bonding on some textiles, residual exfoliated cell components of samples containing rust, and residual exfoliated cell components of samples that have been touched by multiple people; The method for extracting exfoliated cell components is aimed at extracting old samples with strong surface bonding or samples with severe surface weathering, and is performed by peeling and extracting using a peeling tool (4); The exfoliated cell component extraction method is used for extracting residual exfoliated cell components from a sample containing rust, and separates iron ions or ferric oxide by a high-speed centrifuge and membrane filtration.

2. The method for extracting exfoliated cell components based on the exfoliated cell visualization extraction instrument according to claim 1, characterized in that: The stripping tool (4) is a cutting tool.

3. The method for extracting exfoliated cell components based on the exfoliated cell visualization extraction instrument according to claim 2, characterized in that: The stripping tool (4) comprises: A handle (401), a knife bar (402), and a blade (403) fixed to the front end of the knife bar (402); The stripping tool (4) strips off the detached cell components on the surface of old specimens that are firmly bonded to the surface or specimens that are severely weathered, through the blade (403); The peeled off cell component material is then observed by an exfoliated cell visualization extraction instrument and displayed on a display.

4. The method for extracting exfoliated cell components based on the exfoliated cell visualization extraction instrument according to claim 1, characterized in that: When extracting the residual exfoliated cell components from samples containing rust, the specific extraction steps include: S1. Use a high-speed centrifuge to centrifuge rust, iron ions or larger impurities to the bottom or separate them into other layers; S2. After centrifugation, a suspension liquid mixture of relatively clean cells and cell fragments and a certain amount of iron ions in the corresponding part is drawn; S3. Use a filter to repeatedly filter and rinse the suspension to separate cell components and iron ions or metal impurities.

5. The method for extracting exfoliated cell components based on the exfoliated cell visualization extraction instrument according to claim 4, characterized in that: The filters include: filter cup (5); and A funnel (6) connected to the lower end of the filter cup (5); The filter cup (5) and the funnel (6) are connected by a snap connection or a threaded connection; Filter paper (7) is provided at the connection between the filter cup (5) and the funnel (6) and inside the filter cup (5).

6. The method for extracting exfoliated cell components based on the exfoliated cell visualization extraction instrument according to claim 5, characterized in that: The pore size of the filter paper (7) is 500 nm to 1000 nm.

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