Fluorescent illumination method

Abstract

The invention provides a fluorescence illumination method which can generate various excitation lights with different colors and combinations thereof, can enable different excitation lights to be quickly matched with corresponding excitation blocks and can transmit fluorescence with different colors, and the fluorescence illumination method is high in universality and wide in application. A plurality of pluggable excitation blocks are arranged in the circumferential direction of the turntable rotating around the central pivot, and each excitation block comprises an excitation block shell, an excitation light filter, a dichroscope and a fluorescent light filter; the fluorescent light source is arranged at the side part of the turntable and comprises a lens, a plurality of light source dichroscopes and a plurality of LED lamps capable of emitting fluorescent light with different wavelengths; the plurality of light source dichroscopes are positioned on an optical axis extending in the radial direction of the turntable; the at least one LED lamp emits light, the light is reflected and / or transmitted by the light source dichroscope and then passes through the lens along the optical axis to form parallel exciting light, the color of the exciting light is consistent with the color of the exciting light which can pass through the exciting block rotating to the optical axis, and the exciting light passes through the exciting light filter, is reflected by the dichroscope and then is emitted.

Description

Technical Field

[0001] The invention relates to a fluorescence illumination method for illuminating a microscope. Background Art

[0002] CN2016105390686 discloses a multi-channel fluorescent lighting device, which can provide multiple fluorescent lights of different wavelengths and colors and can switch between different fluorescent lights conveniently and quickly. The multi-channel fluorescent lighting device comprises a shell composed of a base and a cover, a pivot fixed inside the shell, a turntable rotating relative to the pivot with the pivot as the axis, a plurality of fluorescent modules uniformly distributed around the turntable, a dovetail seat, and an eyepiece seat; the fluorescent module comprises a support, an excitation light generating unit, and a main light path, the excitation light generating unit comprises a light source, a lens, and an excitation light filter; the main light path comprises a dichroic mirror and a fluorescent filter; the dichroic mirror reflecting the excitation light and transmitting the fluorescent light is 45 degrees to the optical axis of the main light path and the optical axis of the excitation light generated by the excitation light generating unit; the turning wavelength of the dichroic mirror of each fluorescent module is different, so that the fluorescent wavelength transmitted by each fluorescent module is different; when the turntable is rotated, the main light path of the fluorescent module can be in a position coaxial with the dovetail seat and the eyepiece seat.

[0003] The main problem of the existing excitation block is that each fluorescent module is provided with a light source, a lens, an excitation light filter, a dichroic mirror and a fluorescence filter. Therefore, the light source in each fluorescent module is matched with the excitation light filter, the dichroic mirror and the fluorescence filter in the fluorescent module. Each fluorescent module can only emit fluorescence of a specific wavelength, that is, the number of different wavelengths of fluorescence emitted by it is the same as the number of its fluorescent modules. For example, it has six fluorescent modules, each of which has an LED lamp that emits excitation light with a wavelength of 405nm, 488nm, 559nm, 633nm, 647nm and 745nm. If it is necessary to emit an excitation light of 365nm, it cannot be done. If it is necessary to replace one of the six fluorescent modules with a fluorescent module that can emit an excitation light of 365nm, it is often impossible to achieve this because it involves the disassembly of the old fluorescent module, the positioning and installation of the new fluorescent module on the turntable, the installation and debugging of the lens, LED lamp, excitation light filter, dichroic mirror and fluorescence filter in the new fluorescent module, and the power supply connection of the LED lamp in the new fluorescent module. Therefore, its applicability is poor and it is difficult to meet the differentiated needs of different customers.

[0004] In addition, different customers often have different excitation blocks. The excitation block includes an excitation block housing, an excitation light filter, a dichroic mirror, and a fluorescence filter. The excitation light filters in different excitation blocks can transmit excitation light of different colors, and different excitation blocks must be matched with corresponding excitation light of different colors. Existing LED fluorescent lighting modules cannot be quickly matched and installed with different excitation blocks. Summary of the invention

[0005] The purpose of the present invention is to provide a fluorescence illumination method that can generate multiple excitation lights of different colors and their combinations, and enable different excitation lights to be quickly matched with corresponding excitation blocks, and can transmit fluorescence of different colors, which has strong versatility and wide applicability.

[0006] The fluorescent lighting method of the present invention has a plurality of pluggable excitation blocks on the circumference of a turntable rotating around a central pivot, wherein the excitation blocks include an excitation block housing, an excitation light filter, a dichroic mirror, and a fluorescence filter; the excitation light filters in different excitation blocks can transmit excitation light of different colors; the fluorescent light source on the side of the turntable includes a lens, a plurality of light source dichroic mirrors, and a plurality of LED lamps capable of emitting fluorescence of different wavelengths; the lens and the plurality of light source dichroic mirrors are located on the same optical axis extending in the radial direction of the turntable;

[0007] The fluorescent lighting method is that at least one LED lamp emits light, which is reflected and / or transmitted by a light source dichroic mirror and then passes through a lens along an optical axis to form parallel excitation light. The color of the excitation light is consistent with the color of the excitation light that can be transmitted by an excitation block located on the optical axis as the turntable rotates. The excitation light passes through an excitation light filter and is emitted after being reflected by the dichroic mirror.

[0008] In the fluorescent lighting method, the inner end of the cantilever spring close to the center of the turntable is fixed on the turntable, and the outer end of the cantilever spring away from the center of the turntable has a downward bending portion;

[0009] A positioning platform is provided on the side of the excitation block shell, and the rear end face of the positioning platform close to the center of the turntable is a positioning surface in contact with the thrust surface on the turntable, and the upper part of the positioning platform is an inclined surface, and along the radial direction of the turntable from the inside to the outside, the inclined surface has a clamping section with a height gradually decreasing in the axial direction of the turntable; under the downward elastic force of the cantilever spring, the bent portion of the cantilever spring is pressed on the clamping section, and the excitation block moves toward the center of the turntable in the radial direction until the rear end face of the positioning platform contacts the thrust surface, thereby realizing the positioning of the excitation block.

[0010] In the fluorescent lighting method described above, the turntable is connected to a motor that drives it to rotate, the LED lamp is electrically connected to a power supply module that supplies power to it, and the controller is connected to the motor and the power supply module to control the rotation of the motor and the luminous intensity of different LED lamps;

[0011] A plurality of buttons are arranged on the controller, each button corresponds to an excitation block. When a button is pressed, the controller controls the motor to rotate, driving the turntable to rotate, so that the excitation block corresponding to the pressed button rotates to the optical axis. Then, the controller controls the LED lamp to emit light, forming excitation light that can be transmitted through the excitation block located on the optical axis.

[0012] In the fluorescent lighting method, the controller and the power supply module are arranged on the PCB, an interface electrically connected to the PCB is arranged on the housing, and the buttons are arranged on the operation box, which is electrically connected to the PCB through the interface.

[0013] In the fluorescent lighting method, the rotating disk and the fluorescent light source are enclosed in a housing, an entrance and an exit capable of allowing an excitation block to enter and exit are opened on the housing opposite to the outer periphery of the rotating disk, and a cover plate for covering the entrance and the exit is detachably connected to the housing;

[0014] When a key is pressed for a long time, the controller controls the motor to rotate, driving the turntable to rotate, so that the excitation block corresponding to the key pressed for a long time rotates to a position opposite to the entrance and exit.

[0015] In the fluorescent lighting method, the LED lamps include a purple LED lamp, a blue LED lamp, a green LED lamp and a red LED lamp which emit purple light, blue light, green light and red light respectively; the light source dichroic mirror includes a light source dichroic mirror three, a light source dichroic mirror two and a light source dichroic mirror one; the purple light emitted by the purple LED lamp enters the lens after being reflected by the light source dichroic mirror one; the blue light emitted by the blue LED lamp enters the lens after being reflected by the light source dichroic mirror two; the green light emitted by the green LED lamp enters the lens after being reflected by the light source dichroic mirror three and the light source dichroic mirror two and the light source dichroic mirror one; the red light emitted by the red LED lamp enters the lens through the light source dichroic mirror three, the light source dichroic mirror two and the light source dichroic mirror one.

[0016] In the fluorescent lighting method, the purple LED lamp, the blue LED lamp and the green LED lamp are arranged on a heat sink block 1 located on one side of the optical axis, and the red LED lamp is arranged on a heat sink block 2 located on the optical axis; the top surfaces of the heat sink block 1 and the heat sink block 2 are connected to the PCB board, the enclosure is fixed to the bottom surfaces of the heat sink block 1 and the heat sink block 2, the enclosure, the heat sink block 1, the heat sink block 2, the PCB board, and the lens support for fixing the lens form a sealed cover shell, the LED lamp and the light source dichroic mirror are located in the sealed cover shell; the sealed cover shell is located in the outer shell.

[0017] In the fluorescent lighting method described above, a plurality of spaced heat sinks are arranged on the side surfaces of heat sink block 1 and heat sink block 2, a heat sink fan is arranged outside the sealed cover and between heat sink block 1 or heat sink block 2 and the inner wall of the shell, an air inlet of the heat sink fan is communicated with the space between the heat sinks, and an air outlet of the heat sink fan is opposite to an exhaust port opened on the shell; an air inlet is opened on the shell opposite to the end of heat sink block 1 away from the heat sink fan, and when the heat sink fan is working, cooling air enters the shell through the air inlet, passes through the space between the heat sinks, passes through the heat sink fan, and is discharged from the exhaust port.

[0018] In this patent, the light source dichroic mirror is a dichroic mirror belonging to the prior art. Just to distinguish it from the component "dichroic mirror" in the excitation block, we call the component "dichroic mirror" in the fluorescent light source the light source dichroic mirror.

[0019] Beneficial effects of the present invention:

[0020] The present invention separates the fluorescent light source from the excitation block, disassembles the excitation block on a turntable, and disassembles the fluorescent light source and the lens on a housing. By rotating the turntable, the required excitation block can be placed in a position coaxial (optical axis) with the lens and multiple light source dichroic mirrors, which is quick and convenient. Each LED lamp can emit fluorescence of different wavelengths, and multiple LED lamps can emit fluorescence of multiple different wavelengths and different colors; moreover, any two or more LED lamps among the multiple LED lamps emit light together to form excitation light of new colors; and the same LED lamp can change different luminous intensities, so that the color of the mixed excitation light is very rich. In addition, each excitation block can be disassembled and connected to the turntable, and different excitation blocks can be replaced as needed, so that the color of the excitation light that can be transmitted by the excitation block is consistent with the excitation light of different colors mixed by one or more LED lamps. This greatly expands the scope of use of the fluorescent lighting module. At the same time, the excitation block is universal, and different customers may have excitation blocks that can transmit excitation light of different colors. The fluorescent lighting module can conveniently install excitation blocks of different customers and meet the specific requirements of different customers.

[0021] In order to facilitate operation and control, each button corresponds to an excitation block. When a button is pressed, the turntable rotates, so that the excitation block corresponding to the pressed button rotates to the optical axis. Then, the controller controls the LED light to emit light, forming excitation light that can be transmitted by the excitation block located on the optical axis.

[0022] In order to ensure cleanliness and prevent damage to human eyes caused by fluorescence emission, the turntable and fluorescent light source are enclosed inside the shell. In order to facilitate the replacement of the excitation block, a detachable cover plate for covering the entrance and exit of the excitation block is set on the shell. At the same time, when a button is pressed for a long time, the excitation block corresponding to the button rotates to a position opposite to the entrance and exit, so that the corresponding excitation block can be replaced quickly and accurately.

[0023] The positioning platform on the side of the excitation block shell cooperates with the cantilever spring, so that the excitation block can be positioned on the turntable quickly and accurately. The structure is simple, and the operation of plugging and unplugging the excitation block is very convenient.

[0024] Purple light, blue light, green light and red light can be combined arbitrarily, and combined in different proportions to form excitation light of more colors.

[0025] The sealed cover can prevent dust from entering, ensure that the optical path is free from dust interference, and keep the light source dichroic mirror clean and hygienic.

[0026] An air inlet is provided on the shell body opposite to the end of the heat sink block 1 away from the heat sink fan. The air inlet of the heat sink fan is communicated with the gap between the heat sink fins. The air outlet of the heat sink fan is opposite to and communicated with the air outlet provided on the shell body. When the heat sink fan is working, cooling air enters the shell body through the air inlet, passes through the gap between the heat sink fins, passes through the heat sink fan, and is discharged from the air outlet, thereby effectively dissipating the heat of the heat sink block 1 and / or the heat sink block 2. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 , 2 is a general schematic diagram of the fluorescent lighting module.

[0028] Figure 3 This is the overall schematic diagram of the fluorescent lighting module (without the cover, etc.).

[0029] Figure 4 This is the overall schematic diagram of the fluorescent lighting module (minus the base plate, etc.).

[0030] Figure 5 This is the overall schematic diagram of the fluorescent lighting module (minus the base plate, enclosure, etc.).

[0031] Figure 6 This is the overall schematic diagram of the fluorescent lighting module (minus the housing, etc.).

[0032] Figure 7 , 8 It is a schematic diagram of the connection relationship between the heat sink block 1 and the LED lamp.

[0033] Fig. 9 , 10 It is a schematic diagram of the connection relationship between the turntable, excitation block, etc.

[0034] Fig.11 It is a schematic diagram of the connection relationship between the cantilever spring, positioning table, etc.

[0035] Fig.12 , 13 This is a stereogram of the excitation block.

[0036] Fig.14 It is a schematic diagram of the connection between the operation box and the housing.

[0037] In the figure, the housing 1, the upper housing 11, the bottom plate 12, the cuboid 13, the cylinder 14, the inlet and outlet 141, the cover plate 142, the interface 15, the air inlet 18, the air outlet 19,

[0038] Pivot 2,

[0039] Rotating plate 3, spring combination 30, cantilever spring 31, bending portion 311, thrust surface 33,

[0040] Excitation block 4, excitation block housing 41, excitation light filter 42, dichroic mirror 43, fluorescence filter 44, positioning platform 45, pressing section 451, guide section 452, rear end surface 453,

[0041] Fluorescent light source 5, lens 51, light source dichroic mirror 52, light source dichroic mirror 1 521, light source dichroic mirror 2 522, light source dichroic mirror 3 523, LED lamp 53, purple LED lamp 531, blue LED lamp 532, green LED lamp 533, red LED lamp 534, spotlight cover 538, lamp holder 539,

[0042] Sealed housing 6, heat sink 1 61, heat sink 2 62, heat sink 63, enclosure 64, PCB board 65, heat sink fan 66,

[0043] Servo motor 7, synchronous belt 71, eyepiece seat 81, dovetail seat 82,

[0044] Operation box 9, selection key 91, brightness adjustment key 92, power key 93. DETAILED DESCRIPTION

[0045] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0046] See also Figure 1 The outer shell 1 of the fluorescent lighting module shown is roughly a combination of a cuboid 13 and a cylinder 14.

[0047] It includes an upper shell 11 and a bottom plate 12 .

[0048] The pivot 2 is fixed inside the cylinder 14. The rotating disk 3 coaxial with the cylinder 14 is arranged on the pivot so as to rotate relative to the pivot with the pivot as the axis. The rotating disk 3 includes an upper disk and a lower disk which are fixedly connected.

[0049] There are eight excitation blocks 4, each of which is pluggable and arranged around the turntable, and includes an excitation block housing 41, an excitation light filter 42, a dichroic mirror 43, a fluorescence filter 44, and a positioning stage 45. The excitation light filters in different excitation blocks can transmit excitation light of different colors.

[0050] The fluorescent light source 5 is located in the rectangular parallelepiped 13 on the side of the turntable, and includes a lens holder with a lens 51 arranged on the upper shell, three light source dichroic mirrors 52, and four LED lamps 53 that can emit fluorescence of different wavelengths; the lens and multiple light source dichroic mirrors are located on the same optical axis extending in the radial direction of the turntable.

[0051] The LED lamp 53 includes a purple LED lamp 531, a blue LED lamp 532, a green LED lamp 533, and a red LED lamp 534, which emit purple light, blue light, green light, and red light respectively. The purple light emitted by the purple LED lamp 531 has a central wavelength of 365nm and a bandwidth of 50nm. The blue light emitted by the blue LED lamp 532 has a central wavelength of 460nm and a bandwidth of 60nm. The green light emitted by the green LED has a central wavelength of 550nm and a bandwidth of 60nm. The red light emitted by the red LED lamp 534 has a central wavelength of 640nm and a bandwidth of 80nm. Any two or more LED lamps emit light at the same time to form an excitation light of a new color. If the green LED lamp 533 and the red LED lamp 534 emit light at the same time, yellow excitation light is formed. If the purple LED lamp 531, the blue LED lamp 532, the green LED lamp 533, and the red LED lamp 534 emit light at the same time, white excitation light is formed.

[0052] The light source dichroic mirror 52 includes a light source dichroic mirror three 523, a light source dichroic mirror two 522, and a light source dichroic mirror one 521. The purple light emitted by the purple LED lamp is reflected by the light source dichroic mirror one and then enters the lens. The blue light emitted by the blue LED lamp is reflected by the light source dichroic mirror two and then enters the lens through the light source dichroic mirror one. The green light emitted by the green LED lamp is reflected by the light source dichroic mirror three and then enters the lens through the light source dichroic mirror two and the light source dichroic mirror one. The red light emitted by the red LED lamp enters the lens through the light source dichroic mirror three, the light source dichroic mirror two, and the light source dichroic mirror one.

[0053] The purple LED lamp 531, the blue LED lamp 532, and the green LED lamp 533 are arranged on the heat sink 1 61 located on one side of the optical axis, and the red LED lamp 534 is arranged on the heat sink 2 62 located on the optical axis. Each LED lamp 53 includes a lamp bead, a lamp holder 539, and a spotlight cover 538. The lamp bead is fixed on the lamp holder 539, and the lamp holder 539 is directly connected to the heat sink 1 or the heat sink 2. The spotlight cover 538 is fixed on a lamp cover frame fixedly connected to the heat sink 1 or the heat sink 2.

[0054] The top surfaces of heat sink block 1 61 and heat sink block 2 62 are connected to the PCB board, and the enclosure 64 is fixed to the bottom surfaces of heat sink block 1 and heat sink block 2. The enclosure 64, heat sink block 1 61, heat sink block 2 62, PCB board 65, and a lens support for fixing lens 51 form a sealed cover shell 6, and the LED lamp and the light source dichroic mirror are located in the sealed cover shell; the sealed cover shell 6 is located in the outer shell 1.

[0055] An entrance 141 capable of allowing an excitation block to enter and exit is provided on the cylinder 14 opposite to the outer circumference of the turntable, and a cover plate 142 for covering the entrance and exit is detachably connected to the cylinder 14. The cover plate 142 is detachably connected to the housing by screws, and of course the cover plate 142 can also be adsorbed on the cylinder 14 by magnets.

[0056] The excitation block 4 is pluggable and arranged on the turntable in the radial direction of the turntable 3. Eight spring unions 30 are fixed on the turntable in the circumferential direction, and two cantilever springs 31 are arranged on both sides of each spring union. The two adjacent cantilever springs 31 in two adjacent spring unions 30 cooperate with the positioning platforms 45 on both sides of the excitation block shell in an excitation block 4 to fix an excitation block 4. The inner end of the cantilever spring 31 close to the center of the turntable is fixed to the inner end of the spring union, and the outer end of the cantilever spring 31 away from the center of the turntable has a downward bending portion 311; the rear end face 453 of the positioning platform 45 close to the center of the turntable is a positioning surface in contact with the thrust surface 33 on the turntable, and the upper part of the positioning platform is an inclined surface, and along the radial direction of the turntable from the inside to the outside, the inclined surface includes a guide section 452 whose height gradually increases in the axial direction of the turntable and a clamping section 451 that gradually decreases. When the excitation block 4 is pushed radially along the turntable toward the inner side near the center of the turntable 3, under the downward elastic force of the cantilever spring 31, the bent portion 311 of the cantilever spring 31 slides from the guide section 452 to the clamping section 451 until the rear end surface 453 of the positioning platform contacts the thrust surface 33, and the bent portion 311 is pressed against the clamping section 451. When the excitation block 4 needs to be pulled out, the excitation block 4 is pulled radially outward along the turntable to overcome the downward elastic force of the cantilever spring 31, and the bent portion 311 gradually slides from the clamping section 451 to the guide section 452, so that the excitation block 4 can be quickly removed.

[0057] An eyepiece seat 81 is provided on the upper shell 11 for connecting an eyepiece. A dovetail seat 82 is provided on the bottom plate 12 for connecting with an objective lens, etc. The eyepiece seat 81 and the dovetail seat 82 are coaxial up and down.

[0058] A plurality of spaced heat sinks 63 are arranged on the sides of heat sink block 1 61 and heat sink block 2 62. Two heat sink fans 66 are respectively arranged outside the sealed cover shell 6 and between heat sink block 1 and the inner wall of the shell, and between heat sink block 2 and the inner wall of the shell. The air inlet of the heat sink fan is communicated with the interval between the heat sink fins, and the air outlet of the heat sink fan is opposite to the exhaust port 19 opened on the shell. An air inlet 18 is opened on the upper shell opposite to the end of the heat sink block 1 61 away from the heat sink fan. When the heat sink fan is working, cooling air enters the interior of the shell through the air inlet 18, passes through the interval between the heat sink fins, passes through the heat sink fan, and is discharged from the exhaust port 19.

[0059] The PCB board 65 is located in the cuboid 13, the controller and the power supply module are arranged on the PCB board 65, an interface 15 electrically connected to the PCB board is arranged on the upper shell, and different buttons are arranged on the operation box 9, and the operation box 9 is electrically connected to the PCB board through the interface.

[0060] The turntable 3 is connected to a motor arranged on the housing to drive it to rotate, the LED lamp is electrically connected to a power supply module to supply power to it, and the controller is connected to the motor and the power supply module to control the rotation of the motor and the luminous intensity of different LED lamps.

[0061] A servo motor 7 is fixed on the upper shell 11 near the cylinder 14, and the output shaft of the servo motor 7 is connected to the outer periphery of the lower plate of the turntable 3 through a synchronous belt 71 to form a belt drive. The PCB board 65 is electrically connected to the servo motor 7 through a driver.

[0062] The servo motor 7 is in motion, the turntable 3 rotates, and any one of the excitation blocks 4 can be rotated to the position where the excitation light filter and the dichroic mirror in the excitation block are located on the optical axis; at least one LED lamp emits light, and after being reflected and / or transmitted by the light source dichroic mirror, it passes through the lens along the optical axis to form parallel excitation light, and the color of the excitation light is consistent with the color of the excitation light that can be transmitted by the excitation block located on the optical axis, and the excitation light passes through the excitation light filter 42 in the excitation block 4, and after being reflected by the dichroic mirror 43, it passes through the dovetail seat 82 and is emitted. The fluorescence generated by the excitation light excitation of the observed object passes through the dovetail seat 82, passes through the dichroic mirror 43, and then passes through the eyepiece seat 81 to be emitted.

[0063] The buttons mainly include 8 selection keys 91, 2 brightness adjustment keys 92, and 1 power key 93. Each selection key 91 corresponds to an excitation block 4. When a selection key is pressed for a short time, the motor drives the turntable to rotate so that the excitation block corresponding to the pressed selection key rotates to the optical axis, and then controls the corresponding LED lamp to emit light, forming an excitation light that can be transmitted through the excitation block located on the optical axis. When a selection key is pressed for a long time, the motor drives the turntable to rotate so that the excitation block corresponding to the long-pressed selection key rotates to a position opposite to the entrance and exit. At this time, the cover plate can be removed to replace the excitation block. The brightness adjustment key 92 can adjust the brightness of the illuminated LED lamp.

[0064] The protection scope of the present invention includes but is not limited to the above embodiments. The protection scope of the present invention shall be based on the claims. Any replacement, deformation, and improvement of the technology that can be easily thought of by technicians in this field shall fall within the protection scope of the present invention.

Claims

1. Fluorescent lighting method, characterized by: A plurality of pluggable excitation blocks are arranged on the circumference of the turntable rotating around the central pivot, and the excitation blocks include an excitation block housing, an excitation light filter, a dichroic mirror, and a fluorescence filter; the excitation light filters in different excitation blocks can transmit excitation light of different colors; the fluorescence light source on the side of the turntable includes a lens, a plurality of light source dichroic mirrors, and a plurality of LED lamps capable of emitting fluorescence of different wavelengths; The lens and the plurality of light source dichroic mirrors are located on the same optical axis extending in the radial direction of the rotating disk; The fluorescent lighting method is that at least one LED lamp emits light, which is reflected and / or transmitted by a light source dichroic mirror and then passes through a lens along an optical axis to form parallel excitation light. The color of the excitation light is consistent with the color of the excitation light that can be transmitted by an excitation block located on the optical axis as the turntable rotates. The excitation light passes through an excitation light filter and is emitted after being reflected by the dichroic mirror.

2. The fluorescent lighting method according to claim 1, wherein: The inner end of the cantilever spring close to the center of the turntable is fixed on the turntable, and the outer end of the cantilever spring away from the center of the turntable has a downward bending portion; A positioning platform is provided on the side of the excitation block shell, and the rear end face of the positioning platform close to the center of the turntable is a positioning surface in contact with the thrust surface on the turntable, and the upper part of the positioning platform is an inclined surface, and the inclined surface has a clamping section with a height gradually decreasing from the inside to the outside along the radial direction of the turntable; under the downward elastic force of the cantilever spring, the bent portion of the cantilever spring is pressed on the clamping section, and the excitation block moves in the radial direction toward the center of the turntable until the rear end face of the positioning platform contacts the thrust surface, thereby realizing the positioning of the excitation block.

3. The fluorescent lighting method according to claim 1, wherein: The turntable is connected to a motor that drives it to rotate, the LED lamp is electrically connected to a power supply module that supplies power to it, and the controller is connected to the motor and the power supply module to control the rotation of the motor and the luminous intensity of different LED lamps; A plurality of buttons are arranged on the controller, each button corresponds to an excitation block. When a button is pressed, the controller controls the motor to rotate, driving the turntable to rotate, so that the excitation block corresponding to the pressed button rotates to the optical axis. Then, the controller controls the LED lamp to emit light, forming excitation light that can be transmitted through the excitation block located on the optical axis.

4. The fluorescent lighting method according to claim 3, wherein: The controller and the power supply module are arranged on the PCB board, an interface electrically connected to the PCB board is arranged on the shell, and the buttons are arranged on the operation box, and the operation box is electrically connected to the PCB board through the interface.

5. The fluorescent lighting method according to claim 3, wherein: The turntable and the fluorescent light source are enclosed in a shell, and an entrance and exit capable of allowing an excitation block to enter and exit is opened on the shell opposite to the outer periphery of the turntable, and a cover plate for covering the entrance and exit is detachably connected to the shell; When a key is pressed for a long time, the controller controls the motor to rotate, driving the turntable to rotate, so that the excitation block corresponding to the key pressed for a long time rotates to a position opposite to the entrance and exit.

6. The fluorescent lighting method according to claim 5, characterized in that: The LED lamp includes a purple LED lamp, a blue LED lamp, a green LED lamp and a red LED lamp which emit purple light, blue light, green light and red light respectively. The light source dichroic mirror includes a light source dichroic mirror three, a light source dichroic mirror two and a light source dichroic mirror one. The purple light emitted by the purple LED lamp enters the lens after being reflected by the light source dichroic mirror one. The blue light emitted by the blue LED lamp enters the lens through the light source dichroic mirror one after being reflected by the light source dichroic mirror two. The green light emitted by the green LED lamp enters the lens through the light source dichroic mirror two and the light source dichroic mirror one after being reflected by the light source dichroic mirror three. The red light emitted by the red LED lamp enters the lens through the light source dichroic mirror three, the light source dichroic mirror two and the light source dichroic mirror one.

7. The fluorescent lighting method according to claim 6, characterized in that: The purple LED lamp, the blue LED lamp and the green LED lamp are arranged on a heat sink block 1 located on one side of the optical axis, and the red LED lamp is arranged on a heat sink block 2 located on the optical axis; the top surfaces of the heat sink block 1 and the heat sink block 2 are connected to the PCB board, the enclosure is fixed to the bottom surfaces of the heat sink block 1 and the heat sink block 2, the enclosure, the heat sink block 1, the heat sink block 2, the PCB board, and the lens support for fixing the lens form a sealed cover shell, the LED lamp and the light source dichroic mirror are located in the sealed cover shell; the sealed cover shell is located in the outer shell.

8. The fluorescent lighting method according to claim 7, wherein: A plurality of spaced heat sinks are arranged on the sides of the heat sink block 1 and the heat sink block 2, a heat sink fan is arranged outside the sealed cover and between the heat sink block 1 or the heat sink block 2 and the inner wall of the shell, an air inlet of the heat sink fan is communicated with the space between the heat sinks, and an air outlet of the heat sink fan is opposite to an exhaust port opened on the shell; an air inlet is opened on the shell opposite to the end of the heat sink block 1 away from the heat sink fan, and when the heat sink fan is working, cooling air enters the shell through the air inlet, passes through the space between the heat sinks, passes through the heat sink fan, and is discharged from the exhaust port.

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