Indoor optimal cultivation method and system for common bryophytes in alpine meadow

Abstract

The invention belongs to the technical field of plant cultivation, and particularly relates to an indoor optimal cultivation method and system for common bryophytes in alpine meadow, and the method comprises the following steps: collecting bryophyte samples; drying in a dry and dark place, and manually removing macroscopic impurities; crushing the dried moss sample into stem and leaf fragments with the length of 1-2mm by using a crusher to prepare an inoculation material, and uniformly mixing for later use; setting cultivation conditions; the nutrient soil subjected to high-temperature sterilization serves as a culture medium to be filled in a nutrition bowl with the caliber being 12 cm, 15 g of inoculation materials are scattered, and cultivation is conducted respectively; measuring the moss coverage and the plant density; measuring the thickness of the formed moss crust by using a vernier caliper, measuring the absorbance of the bryophyte under different wavelengths by using an ultraviolet spectrophotometer, and calculating the content of chlorophyll a and exopolysaccharide by using a formula. The bryophytes are cultivated indoors according to experimental design, the optimal cultivation conditions of the bryophytes can be obtained, data support can be provided for rapid propagation of the alpine meadow bryophytes, basic materials are provided for related research of the alpine meadow bryophytes, and the method has important significance.

Description

Technical Field

[0001] The present invention belongs to but is not limited to the field of plant cultivation technology, and in particular relates to an optimal indoor cultivation method and system for common moss plants in alpine meadows. Background Art

[0002] Alpine meadows are the largest grassland type in my country and the most important grassland type on the Qinghai-Tibet Plateau, accounting for 65% of Qinghai's natural grassland area. However, in recent years, the soil and vegetation have degraded to varying degrees, and forage production has declined, affecting the ecological security of my country and even Asia, as well as the quality of life of herders on the Qinghai-Tibet Plateau.

[0003] Although traditional degraded grassland restoration measures have obvious short-term effects, the productivity and ecological functions of restored grasslands are not sustainable, and secondary or even tertiary degradation is prone to occur. In addition, the restoration costs and prices are high. Sustainable alpine meadow restoration methods need further research.

[0004] Moss plants are pioneer plants in the succession of natural communities and an important component of alpine meadows. They play an important role in improving grassland soil environment and maintaining fertility. They can be considered as plant materials for the restoration of degraded grassland plant communities and ecological functions, and can play their role in maintaining water and soil and improving nutrients, promote the restoration of degraded alpine meadows, and explore sustainable degraded alpine meadow restoration technologies and systems.

[0005] There is a lack of relevant research on alpine meadow bryophytes. The material collection cost of bryophytes in related research work is high and is restricted by seasons, which seriously affects the progress of related research work.

[0006] Screening the best indoor cultivation conditions for alpine meadow bryophytes can lay the foundation for the rapid cultivation and expansion of bryophytes, provide convenience and reduce costs for related research on bryophytes, and provide basic material guarantees for the use of bryophytes in alpine meadow restoration, thereby promoting related research on alpine meadow bryophytes and realizing sustainable restoration by combining them as exogenous additives with other traditional restoration measures in the wild. However, there is currently a lack of research on alpine meadow bryophytes and a lack of cultivation methods. Summary of the invention

[0007] In view of the problems existing in the prior art, the present invention provides an optimal indoor cultivation method and system for common moss plants in alpine meadows.

[0008] The present invention is achieved by providing an optimal indoor cultivation method for common moss plants in alpine meadows, the method comprising:

[0009] S1: Common bryophyte samples were collected from alpine meadows in Henan Mongolian Autonomous County, Huangnan Tibetan Autonomous Prefecture, Qinghai Province;

[0010] S2: Bring the moss samples back to the laboratory, place them in a dry and dark place to dry, and manually remove impurities such as plant residues, soil, and stones that are visible to the naked eye;

[0011] S3: Use a grinder to crush the shade-dried moss sample into stem and leaf fragments with a length of 1-2 mm to prepare inoculation materials, which are mixed and set aside;

[0012] S4: Set 9 cultivation conditions based on four factors: light intensity lx, photoperiod h, temperature °C and air humidity %;

[0013] S5: The sterilized nutrient soil was used as the culture medium to fill the nutrient pot with a diameter of 12 cm, and 15 g of inoculation material was spread, and the culture was carried out in the artificial climate incubator under 9 culture conditions;

[0014] S6: moss cover and plant density were measured every 8 days;

[0015] S7: After 40 days of cultivation, the thickness of the moss crust was measured with a vernier caliper, the absorbance of the moss plants at different wavelengths was measured with an ultraviolet spectrophotometer, and the chlorophyll a and extracellular polysaccharide contents were calculated using a formula to characterize the biomass and metabolic capacity of the moss plants.

[0016] Further, the 9 cultivation conditions are 2000lx+10h+10℃+65%, 2000lx+12h+15℃+75%, 2000lx+14h+20℃+85%, 3000lx+10h+15℃+85%, 3000lx+12h+20℃+65%, 3000lx+14h+10℃+75%, 4000lx+10h+20℃+75%, 4000lx+12h+10℃+85% and 4000lx+14h+15℃+65%.

[0017] Another object of the present invention is to provide an optimal indoor cultivation method and system based on the common moss plants in the alpine meadow, the system specifically comprising:

[0018] The collection module is used to collect common bryophyte samples in the alpine meadows of Henan Mongolian Autonomous County, Huangnan Tibetan Autonomous Prefecture, Qinghai Province;

[0019] The pretreatment module is connected to the collection module, and the moss samples are brought back to the laboratory, placed in a dry and dark place to dry, and the impurities such as plant residues, soil blocks, and stones visible to the naked eye are manually removed; the shade-dried moss samples are crushed into stem and leaf fragments with a length of 1-2 mm using a crusher to prepare inoculation materials, which are mixed and set aside;

[0020] The cultivation condition setting module is connected to the pre-processing module and is used to set 9 cultivation conditions according to four factors: light intensity lx, photoperiod h, temperature °C and air humidity %.

[0021] The cultivation module is connected to the cultivation condition setting module, and is used to fill the nutrient pot with a diameter of 12 cm with the high-temperature sterilized nutrient soil as the cultivation matrix, and spread 15g of inoculation material, and cultivate in the artificial climate incubator under various conditions; the moss coverage and plant density are measured every 8 days; when the cultivation is completed at 40 days, the thickness of the formed moss crust is measured with a vernier caliper, and the absorbance of the moss plant at different wavelengths is measured with an ultraviolet spectrophotometer, and the chlorophyll a and extracellular polysaccharide contents are calculated using the formula to characterize the biomass and metabolic capacity of the moss plant.

[0022] Furthermore, the spectrum range of the adjustable LED light source is 400-700nm, simulating the natural light environment to promote the photosynthesis of moss plants and ensure that the plants obtain the best lighting conditions at different growth stages.

[0023] Furthermore, the nutrient soil treated with high temperature sterilization is taken out from the autoclave, placed at room temperature and then inoculated to improve the survival rate and growth rate of the inoculated material.

[0024] Furthermore, humus and organic fertilizer are added to the nutrient soil, with the humus content being 10%-20% and the organic fertilizer content being 5%-10%, so as to improve the water retention and air permeability of the soil and promote the rapid growth and root development of the moss plants.

[0025] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are as follows:

[0026] First, the present invention cultivates bryophytes indoors according to the experimental design, can obtain the best cultivation conditions for bryophytes, and can provide data support for the rapid propagation of bryophytes in alpine meadows, which is of great significance.

[0027] (1) The restoration of bare patches is a difficult point in the ecological restoration of alpine meadows. Compared with non-degraded alpine meadows, bare patches have no turf layer and the ground is bare, which is very prone to nutrient and soil loss. Mosses are pioneer plants in natural community succession and an important component of alpine meadows. They can cover the bare ground well, reduce soil erosion, promote soil nutrient circulation, and improve the soil environment. They can be considered as plant materials for the restoration of degraded grassland plant communities and ecological functions, and play their role in maintaining soil and improving nutrients, thereby promoting the restoration of degraded alpine meadows.

[0028] (2) The successful cultivation and propagation of mosses indoors is an important condition for in-depth understanding of the role and mechanism of mosses in alpine meadow ecosystems, and is also a necessary condition for using mosses to restore degraded meadows.

[0029] (3) Although there have been some studies on the cultivation methods of mosses, the alpine meadow distribution area has high altitude, cold climate, strong light, and the plant growth environment conditions are very different from those in other regions. The existing moss cultivation methods are not suitable for the cultivation of alpine meadow mosses. Therefore, the lack of alpine meadow moss cultivation methods has seriously restricted the research and utilization of alpine meadow mosses.

[0030] (4) This study targeted the special altitude, temperature, light and moisture conditions in alpine areas. The cultivation methods of alpine meadow bryophytes were studied under different combinations of light intensity lx, photoperiod h, temperature ℃ and air humidity %. The optimal conditions suitable for the cultivation of bryophytes in alpine areas were screened out, providing breeding conditions and basis for the application of bryophytes in alpine areas.

[0031] Second, the expected benefits and commercial value of the technical solution of the present invention after transformation are:

[0032] The present invention will provide basic materials and methods for indoor and outdoor research related to mosses in alpine areas, and also provide possibilities for the commercial utilization of mosses.

[0033] The technical solution of the present invention fills the technical gap in the industry at home and abroad:

[0034] The invention fills the blank of indoor culture and breeding methods of moss plants in high-cold areas.

[0035] Third, the present invention proposes an optimal cultivation method for indoor cultivation of common mosses in alpine meadows, aiming to solve the problems of difficulty in cultivating mosses from alpine areas in artificial environments, long cultivation cycles, and low survival rates in the prior art. By rationally controlling environmental parameters such as light, temperature, and humidity, the present invention significantly improves the cultivation success rate of mosses, shortens the growth cycle, and ensures the growth quality of mosses.

[0036] 1. Optimization parameters of light intensity, photoperiod, temperature and humidity

[0037] The present invention designs nine different combinations of light intensity, photoperiod, temperature and humidity to determine the most suitable environmental conditions for indoor cultivation of moss. These parameters include:

[0038] Light intensity: 2000lx, 3000lx, 4000lx

[0039] Photoperiod: 10h, 12h, 14h

[0040] Temperature: 10℃, 15℃, 20℃

[0041] Humidity: 65%, 75%, 85%

[0042] Technical problem solving: In traditional methods, there is a lack of systematic research on the cultivation conditions of moss plants, and usually only rely on experience to control light and temperature, resulting in unstable survival rates. By systematically setting and testing nine groups of cultivation conditions, the present invention not only solves the problem that moss growth is sensitive to environmental conditions, but also determines the optimal combination to ensure that moss can grow normally under artificial conditions, breaking through the limitation of previous cultivation technology relying on natural conditions.

[0043] Significant technical progress: Through the optimized combination of light, photoperiod, temperature and humidity, the present invention significantly improves the growth rate and survival rate of moss plants. Compared with the traditional natural environment cultivation method, the cultivation period is shortened and the growth state of moss is more stable.

[0044] 2. Optimization of moss inoculation materials

[0045] The invention crushes the collected moss samples into stem and leaf fragments of 1-2 mm to prepare uniform inoculation materials. The crushing process increases the surface area of ​​the moss, facilitates the moss to fully contact with the nutrient soil, and promotes the uniform growth of the moss.

[0046] Technical problem solved: In traditional methods, the processing of moss samples is relatively extensive, usually directly sowing or transplanting in large pieces, resulting in uneven nutrient supply and unstable growth. The present invention solves the problem of uneven inoculation materials by crushing the shade-dried moss samples, ensuring the rapid rooting and germination of the moss.

[0047] Significant technological progress: Optimized inoculation material processing technology improves the efficiency of moss cultivation, allowing moss at each inoculation point to obtain uniform growth conditions, further improving survival rate and growth rate. This innovation greatly simplifies the operation process of indoor moss cultivation and reduces the waste of resources caused by uneven inoculation.

[0048] 3. Environmental control and monitoring system

[0049] During the cultivation process, the present invention uses an artificial climate incubator to strictly control environmental conditions, and measures the coverage and plant density of the moss every 8 days during the cultivation period, measures the thickness of the moss crust after 40 days, and uses an ultraviolet spectrophotometer to measure the chlorophyll a content and extracellular polysaccharide content to ensure the healthy growth of the moss.

[0050] Technical Problem Solved: Traditional moss cultivation methods lack sophisticated environmental monitoring and control methods, often resulting in long growth cycles and unstable health conditions. The present invention uses an artificial climate incubator to precisely control key parameters such as light, temperature, and humidity, while also combining regular measurements of moss growth indicators to solve the problem of not being able to timely grasp the growth status of moss.

[0051] Significant technological progress: Through the combination of environmental control and monitoring, the present invention not only ensures the best growth conditions for mosses, but also monitors their growth status in real time and adjusts the cultivation plan in time. This refined management method significantly improves the success rate of moss cultivation and provides a reliable reference standard for indoor cultivation of other mosses.

[0052] 4. Detection of crust thickness and chlorophyll a content

[0053] The invention introduces an ultraviolet spectrophotometer to measure the chlorophyll a content and the extracellular polysaccharide content of the moss plant, and measures the crust thickness at the end of cultivation as an evaluation index of the growth effect of the moss plant.

[0054] Technical Problem Solved: In traditional moss cultivation, there is a lack of effective means for evaluating growth effects, and the health status of moss is often judged based on experience. The present invention uses scientific detection methods to accurately quantify the growth effect and health status of moss, filling the gap in the existing technology in effect evaluation.

[0055] Significant technological progress: Through scientific detection methods, the present invention provides quantitative data on the growth status of moss, providing a reliable basis for the improvement and optimization of subsequent cultivation. This evaluation method based on scientific data not only improves the cultivation efficiency, but also provides a standardized operating procedure for large-scale moss cultivation.

[0056] The present invention significantly improves the indoor cultivation efficiency and success rate of alpine meadow bryophytes by optimizing key environmental parameters such as light, temperature, and humidity, finely processing inoculated materials, and combining scientific environmental monitoring and growth effect detection methods. Compared with the prior art, the present invention not only solves multiple problems in bryophyte cultivation, but also realizes the standardization and automation of the cultivation process, which is a significant technological advancement. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 This is a flow chart of an optimal indoor cultivation method for common mosses in alpine meadows provided by an embodiment of the present invention;

[0058] Figure 2 This is a structural diagram of an optimal indoor cultivation system for common mosses in alpine meadows provided by an embodiment of the present invention;

[0059] Figure 3The moss coverage changes under different cultivation conditions provided by the embodiments of the present invention;

[0060] Figure 4 The embodiment of the present invention provides a change in the density of moss strains under different cultivation conditions;

[0061] Figure 5 It is the growth change of moss under the conditions of 2000lx+14h+20℃+85% provided by the embodiment of the present invention;

[0062] Figure 6 It is the content of the optimal cultivation condition evaluation index provided by the embodiment of the present invention;

[0063] Figure 7 is a diagram showing the growth of bryophytes under optimal cultivation conditions provided by an embodiment of the present invention;

[0064] Figure 8 This is a diagram showing an example of application of the cultivated moss plant provided by an embodiment of the present invention in an indoor research experiment;

[0065] Fig. 9 This is a diagram showing the growth of the cultivated bryophyte in a natural grassland provided by an embodiment of the present invention;

[0066] In the figure: 1. Acquisition module; 2. Preprocessing module; 3. Cultivation condition setting module; 4. Cultivation module. DETAILED DESCRIPTION

[0067] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0068] like Figure 1 As shown, an embodiment of the present invention provides an optimal indoor cultivation method for common mosses in alpine meadows, the method comprising:

[0069] S1: Common bryophyte samples were collected from alpine meadows in Henan Mongolian Autonomous County, Huangnan Tibetan Autonomous Prefecture, Qinghai Province;

[0070] S2: Bring the moss samples back to the laboratory, place them in a dry and dark place to dry, and manually remove impurities such as plant residues, soil, and stones that are visible to the naked eye;

[0071] S3: Use a grinder to crush the shade-dried moss sample into stem and leaf fragments with a length of 1-2 mm to prepare inoculation materials, which are mixed and set aside;

[0072] S4: Set 9 cultivation conditions based on four factors: light intensity lx, photoperiod h, temperature °C and air humidity %;

[0073] S5: Fill the nutrient pots with a diameter of 12 cm with high-temperature sterilized nutrient soil as the cultivation medium, and spread 15 g of inoculation material, and cultivate them in artificial climate incubators under various conditions;

[0074] S6: moss cover and plant density were measured every 8 days;

[0075] S7: After 40 days of cultivation, the thickness of the moss crust was measured with a vernier caliper, the absorbance of the moss plants at different wavelengths was measured with an ultraviolet spectrophotometer, and the chlorophyll a and extracellular polysaccharide contents were calculated using a formula to characterize the biomass and metabolic capacity of the moss plants.

[0076] Collect common moss samples in alpine meadows and select healthy and representative samples. After bringing these samples back to the laboratory, place them in a dry, dark environment to dry, avoiding direct exposure to strong light that would affect the activity of the moss samples. During the drying process, manually remove impurities visible to the naked eye, such as plant residues, soil clods, and stones, to ensure the purity of the samples and provide standardized inoculation materials for subsequent experiments.

[0077] The dried moss samples were crushed. The moss was crushed into stem and leaf fragments of about 1-2 mm in length by a crusher to ensure that they were of moderate size, suitable for sowing and conducive to nutrient absorption and subsequent growth. The crushed moss stem and leaf fragments were used as inoculation materials. In order to ensure the uniformity of the experiment, the crushed samples needed to be fully mixed for use to ensure that the inoculation materials were evenly distributed under various cultivation conditions.

[0078] In the artificial climate incubator, 9 different cultivation conditions are set by precisely controlling four factors, namely, light intensity (lx), photoperiod (h), temperature (℃) and air humidity (%). These conditions reflect the growth response of mosses in different environments. By changing these variables, the indoor cultivation environment of mosses can be optimized, thereby determining the most suitable growth conditions and improving their growth efficiency.

[0079] Use high temperature sterilized nutrient soil as the cultivation matrix for moss to prevent interference from bacteria or other microorganisms. Fill the nutrient soil with a diameter of 12 cm. The nutrient soil not only provides the nutrients needed for moss growth, but also ensures its healthy growth in a sterile environment after sterilization. Sprinkle 15g of crushed inoculation material evenly on the surface of each nutrient pot so that the moss can evenly cover the soil surface and be artificially cultivated under different conditions.

[0080] The moss coverage and plant density are measured every 8 days, and the growth rate and growth trend are evaluated by observing and recording the moss growth coverage area and the number of plants per unit area. These indicators help to judge the growth of moss under different cultivation conditions, find the light, temperature, and humidity combination conditions that are most suitable for the growth of moss plants, and provide reference data for subsequent optimization.

[0081] After 40 days of cultivation, the thickness of the moss crust was measured by a vernier caliper, the absorbance of the moss was measured at different wavelengths using an ultraviolet spectrophotometer, and the chlorophyll a content and exopolysaccharide content of the moss were calculated using a formula. The chlorophyll a and exopolysaccharide content of the moss represent its biomass and metabolic capacity. These data are used to evaluate the growth quality and physiological state of the moss under different cultivation conditions, and then determine the best indoor cultivation method.

[0082] The 9 cultivation conditions are 2000lx+10h+10℃+65%, 2000lx+12h+15℃+75%, 2000lx+14h+20℃+85%, 3000lx+10h+15℃+85%, 3000lx+12h+20℃+65%, 3000lx+14h+10℃+75%, 4000lx+10h+20℃+75%, 4000lx+12h+10℃+85% and 4000lx+14h+15℃+65%.

[0083] The optimal indoor cultivation method for common moss plants in alpine meadows provided by the present invention first collects moss samples from alpine meadows through step S1 and brings them back to the laboratory for processing. The collected moss samples need to be dried in a dry and dark place to avoid direct sunlight from damaging the moss structure. After drying, impurities such as plant residues, soil blocks and stones are manually removed to ensure the purity of the moss samples, providing a good foundation for subsequent crushing and cultivation steps.

[0084] In step S2, the dried moss samples are crushed into 1-2 mm stem and leaf fragments, which helps to increase the surface area of ​​the moss inoculation material and promote its uniform distribution and rapid germination and growth in the nutrient soil. After the inoculation material is processed by the crusher, it is mixed evenly and set aside to ensure that all parts of the moss can receive sufficient light and nutritional support during the cultivation process.

[0085] In step S3, nine different cultivation conditions of light, photoperiod, temperature and humidity are set to simulate the various growth states of moss in the natural environment. The combination of these conditions can help determine the light intensity, photoperiod, temperature and humidity that are most suitable for indoor cultivation. For example, the light intensity ranges from 2000lx to 4000lx, the photoperiod ranges from 10 to 14h, the temperature is set at 10 to 20°C, and the air humidity ranges from 65% to 85%. By controlling these conditions, the photosynthesis, nutrient absorption and growth rate of moss can be optimized.

[0086] In step S4, the nutrient soil that has been sterilized at high temperature is placed in the nutrient pot to ensure that the moss grows in a sterile environment. After the inoculated material is evenly spread, it is placed in an artificial climate incubator for cultivation. During the cultivation period, the coverage and plant density of the moss need to be measured every 8 days, the crust thickness of the moss is measured after 40 days, and the chlorophyll a content and extracellular polysaccharide content are measured using an ultraviolet spectrophotometer. These measurement data can help researchers monitor the growth status and health status of the moss, evaluate the effects under different cultivation conditions, and thus determine the optimal indoor moss cultivation method.

[0087] like Figure 2 As shown, the embodiment of the present invention provides an optimal indoor cultivation system for common bryophytes in alpine meadows based on the optimal indoor cultivation method for common bryophytes in alpine meadows, and the system specifically includes:

[0088] Collection module 1, used to collect common bryophyte samples in the alpine meadow of Henan Mongolian Autonomous County, Huangnan Tibetan Autonomous Prefecture, Qinghai Province;

[0089] The pretreatment module 2 is connected to the collection module 1, and the moss sample is brought back to the laboratory, placed in a dry and dark place to dry, and the impurities such as plant residues, soil blocks, and stones visible to the naked eye are manually removed; the shade-dried moss sample is crushed into stem and leaf fragments with a length of 1-2 mm by a crusher to prepare the inoculation material, which is mixed and set aside;

[0090] The cultivation condition setting module 3 is connected to the pre-processing module 2 and is used to set 9 cultivation conditions according to four factors: light intensity lx, photoperiod h, temperature °C and air humidity %.

[0091] The cultivation module 4 is connected to the cultivation condition setting module 3, and is used to fill the nutrient pot with a diameter of 12 cm with the high-temperature sterilized nutrient soil as the cultivation matrix, and spread 15g of inoculation material, and cultivate in the artificial climate incubators under 9 cultivation conditions respectively; the moss coverage and plant density are measured every 8 days; when the cultivation is completed at 40 days, the thickness of the formed moss crust is measured with a vernier caliper, and the absorbance of the moss plant at different wavelengths is measured with an ultraviolet spectrophotometer, and the chlorophyll a and extracellular polysaccharide contents are calculated using a formula to characterize the biomass and metabolic capacity of the moss plant.

[0092] Relevant evidence of the technical effects achieved by the embodiments of the present invention.

[0093] (1) Changes in moss coverage under different cultivation conditions, such as Figure 3 shown.

[0094] Under the cultivation conditions of 2000lx+14h+20℃+85% and 4000lx+12h+10℃+85%, the moss plants grew rapidly, and the coverage increased significantly faster than other cultivation conditions, and the moss growth performance was the best. At 24 days of cultivation, the moss coverage reached 81% under the conditions of 2000lx+14h+20℃+85%, and the moss coverage reached the highest level of 88% under the conditions of 4000lx+12h+10℃+85%, which was much higher than other cultivation conditions; at the end of 40 days of cultivation, it reached 97% and 96% respectively. At 24 days of cultivation, the coverage was much higher than that of other cultivation treatments, and the rapid growth period under this condition was completed, and it entered a stable growth period, that is, the coverage only increased by 12%-16% during the cultivation process of 24d-40d, while other treatments were still in a slow linear growth period. Therefore, from the perspective of coverage, cultivating for 24 days under these two conditions can achieve the purpose of rapid breeding.

[0095] (2) Changes in moss plant density under different cultivation conditions, such as Figure 4 shown.

[0096] With the extension of cultivation time, the change trend of moss plant density and coverage is basically the same. At 24 days of cultivation, compared with other cultivation conditions, the moss plant density reached the highest under the conditions of 4000lx+12h+10℃+85%, which was 7.08 plants / cm 2 , the second highest under 2000lx+14h+20℃+85% condition was 4.33 plants / cm 2 The change trend of moss strain density was the same as that of the culture time and when the culture was completed at 40 days, which was the highest under the two culture conditions.

[0097] (3) Changes in moss growth under 2000lx+14h+20℃+85%, such as Figure 5 shown.

[0098] (4) The content of the evaluation indexes for the optimal cultivation conditions, such as Figure 6 shown.

[0099] After 40 days of cultivation, the moss coverage and crust thickness reached the highest under the conditions of 2000lx+14h+20℃+85%, which were 97% and 6.93mm respectively, followed by 4000lx+12h+10℃+85%, which were 96% and 6.25mm respectively. The moss plant density reached the highest under the conditions of 4000lx+12h+10℃+85%, which was 7.50 plants / cm 2 The second highest value was 6.83 plants / cm under the condition of 2000lx+14h+20℃+85%. 2 .

[0100] The chlorophyll a content was highest under 4000lx+12h+10℃+85%, significantly higher than other treatments, while the extracellular polysaccharide content was highest under 4000lx+10h+20℃+75%, followed by 2000lx+14h+20℃+85%, but the difference between the two was not significant.

[0101] (5) Growth of bryophytes under optimal cultivation conditions, such as Figure 7 shown.

[0102] (6) Examples of the application of cultivated mosses in indoor research experiments, such as Figure 8 shown.

[0103] Through the indoor cultivation and propagation of mosses, basic materials can be provided for the relevant research on alpine meadow bryophytes. First of all, there is a lack of relevant theoretical basic research on alpine meadow bryophytes. All related research requires researchers to go to the alpine areas to collect them in person, which is costly. Secondly, due to special environmental conditions, the season for collecting mosses is limited to the growing season, and the growing season on the Qinghai-Tibet Plateau is short, which greatly limits the implementation process and research cycle of related research. If the materials for the propagation of mosses are commercialized, it will facilitate the conduct of related research and reduce time and financial costs. Figure 8 This is an example of applying cultivated alpine meadow bryophytes to the study of the interaction between bryophytes and forage grasses.

[0104] (7) Growth of mosses in natural grasslands, such as Fig. 9 (Left) As shown; the cultivated moss plants can be applied to the research and practice of alpine meadow ecological restoration, such as Fig. 9 (right) shown.

[0105] Moss plants are an important component of alpine meadows. However, in patchy and degraded alpine meadows, the turf is destroyed, the ground is bare, and soil and water are lost. If mosses are inoculated into the bare patches, they can play a role in conserving water and soil and improving fertility, and can provide new materials for the restoration of degraded meadows. Fig. 9 This is an experiment on inoculating mosses in bare patches of alpine meadow.

[0106] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. An optimal indoor cultivation method for common mosses in alpine meadows, characterized in that: The method includes: S1: Collect common moss samples in alpine meadows, bring them back to the laboratory, dry them in a dry and dark place, and manually remove plant residues, soil blocks, and stone impurities; S2: crush the dried moss sample into 1-2 mm stem and leaf fragments to prepare inoculation material; S3: setting nine cultivation conditions of light intensity, photoperiod, temperature and air humidity; S4: Put the high-temperature sterilized nutrient soil into the nutrient pot, spread the inoculation material, and cultivate it in an artificial climate incubator.

2. The optimal indoor cultivation method for common mosses in alpine meadows according to claim 1, characterized in that: The moss samples were crushed into stem and leaf fragments with a length of 1-2 mm by a crusher, and then mixed and used as inoculation materials for later use.

3. The optimal indoor cultivation method for common mosses in alpine meadows according to claim 1, characterized in that: The nine cultivation conditions are 2000lx+10h+10℃+65%, 2000lx+12h+15℃+75%, 2000lx+14h+20℃+85%, 3000lx+10h+15℃+85%, 3000lx+12h+20℃+65%, 3000lx+14h+10℃+75%, 4000lx+10h+20℃+75%, 4000lx+12h+10℃+85%, 4000lx+14h+15℃+65%.

4. The optimal indoor cultivation method for common mosses in alpine meadows according to claim 1, characterized in that: The method further comprises measuring the moss coverage and plant density every 8 days during the cultivation period, measuring the thickness of the moss crust after 40 days, and measuring the chlorophyll a content and the extracellular polysaccharide content by using an ultraviolet spectrophotometer.

5. The optimal indoor cultivation method for common mosses in alpine meadows according to claim 1, characterized in that: The adjustable LED light source has a spectrum range of 400-700 nm, simulating a natural light environment to promote photosynthesis of mosses and ensure that plants obtain optimal lighting conditions at different growth stages.

6. The optimal indoor cultivation method for common mosses in alpine meadows according to claim 1, characterized in that: The moss samples sterilized by ultraviolet high temperature are subjected to room temperature recovery treatment for 24 hours after sterilization to ensure that the moisture and activity of the moss samples are restored, so as to improve the survival rate and growth rate of the inoculated materials.

7. The optimal indoor cultivation method for common mosses in alpine meadows according to claim 1, characterized in that: Humus and organic fertilizer are added to the nutrient soil, with the humus content being 10%-20% and the organic fertilizer content being 5%-10%, so as to improve the water retention and air permeability of the soil and promote the rapid growth and root development of the moss plants.

8. An optimal indoor cultivation system for common bryophytes in alpine meadows based on the optimal indoor cultivation method for common bryophytes in alpine meadows as claimed in claims 1 to 7, characterized in that: The system specifically includes: The collection module is used to collect common bryophyte samples in the alpine meadows of Henan Mongolian Autonomous County, Huangnan Tibetan Autonomous Prefecture, Qinghai Province; The pretreatment module is connected to the collection module, and the moss samples are brought back to the laboratory, placed in a dry and dark place to dry, and the plant residues, soil blocks, and stone impurities visible to the naked eye are manually removed; the shade-dried moss samples are crushed into stem and leaf fragments with a length of 1-2 mm using a crusher to prepare inoculation materials, which are mixed and set aside; The cultivation condition setting module is connected to the pre-processing module and is used to set 9 cultivation conditions according to four factors: light intensity lx, photoperiod h, temperature °C and air humidity %. The cultivation module is connected to the cultivation condition setting module, and is used to fill the nutrient pot with a diameter of 12 cm with the high-temperature sterilized nutrient soil as the cultivation matrix, and spread 15g of inoculation material, and cultivate in the artificial climate incubator under various conditions; the moss coverage and plant density are measured every 8 days; after 40 days of cultivation, the thickness of the formed moss crust is measured with a vernier caliper, and the absorbance of the moss plant at different wavelengths is measured with an ultraviolet spectrophotometer, and the chlorophyll a and extracellular polysaccharide contents are calculated using the formula to characterize the biomass and metabolic capacity of the moss plant.

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