Equipment-free procambarus clarkii indoor temporary culture method

Abstract

The invention discloses a device-free indoor temporary rearing method for procambarus clarkii. The method comprises management and control of a container, a water body, a support, room temperature, volume, illumination, density, feeding and the like. According to the method, professional aquatic equipment and fine management are not needed, the principle is scientific, operation is easy, copying can be achieved, the survival rate of the procambarus clarkii bred indoors can be remarkably increased, the requirements for material taking, observation and appreciation of most experiments can be met, the death rate of the procambarus clarkii bred indoors can be remarkably reduced, the survival rate is greatly increased, the 3-month survival rate exceeds 80%, and the survival rate of the procambarus clarkii bred indoors is greatly increased. The 6-month survival rate exceeds 60%.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to an equipment-free indoor temporary culture method for Procambarus clarkii. Background Art

[0002] Procambarus clarkii, commonly known as crayfish, belongs to the class Crustacea, order Decapoda, family Crayfish, and is an edible freshwater crayfish with delicious meat. In recent years, the artificial breeding volume and market consumption of Procambarus clarkii have increased dramatically.

[0003] Due to the strong market effect, a series of research booms have been driven on basic research, feed processing, disease prevention, and genetic breeding of Procambarus clarkii. Different from the breeding conditions of aquatic organisms such as shrimps and crabs, if samples are taken from the wild and kept directly in the laboratory, the survival rate of individuals will be less than 50% within 3 days and basically drop to 0 within 7 days without taking measures. This is related to the basic physiological characteristics of crayfish, such as dislike of strong light, love of digging holes, molting growth, living on land and water, fighting for victory, territorial awareness, and hatching eggs, which all put forward requirements for indoor breeding, so the mortality rate is high and it is difficult to survive.

[0004] In addition, due to the particularity of indoor research work such as laboratories, it is required to continuously track the temporarily cultured crayfish indoors, often over a period of several weeks or months; and the treatment of crayfish is often required to be a control analysis between multiple parallel groups and multiple parallel individuals, which also determines that the culture environment between groups should be as consistent as possible to reduce experimental errors. In the prior art, strict temporary culture conditions for crayfish are achieved through specially set equipment, such as a patent application with publication number CN111700012A, which discloses a method for improving the survival rate of laboratory cultured crayfish, and constructs a special culture system including a reservoir, a water pump, a cooling and heating machine, an aeration pump and an air conditioner. Customized equipment can provide adaptive survival conditions for laboratory culture of crayfish, but the customized equipment system structure is complicated, which also increases the difficulty of subsequent operations, and the complicated equipment system will also affect the quality of life of crayfish.

[0005] Therefore, one of the prerequisites for conducting research in the laboratory is how to ensure that reproducible environmental conditions are created in a simple operating environment to maintain the life characteristics and quality of life of living crayfish. Furthermore, it is urgent to provide a laboratory temporary culture method that fully guarantees the survival quality of crayfish. Summary of the invention

[0006] In view of the deficiencies of the above-mentioned prior art, the present invention provides an equipment-free indoor temporary culture method for Procambarus clarkii, aiming to solve the technical problem that in the prior art, specially configured equipment is used to achieve strict temporary culture conditions for Procambarus clarkii, but the structure of the above-mentioned customized equipment system is complicated, which also increases the difficulty of subsequent operations, and the complicated equipment system will also affect the quality of life of Procambarus clarkii.

[0007] The present invention provides an equipment-free indoor temporary culture method for Procambarus clarkii, which comprises adding culture water into a temporary culture container, placing Procambarus clarkii for culture, and managing the culture; the culture management includes the following contents:

[0008] (1) Water management: The water level of the aquaculture water body shall not exceed 10 cm;

[0009] (2) Temperature management: the water temperature is adjusted by room temperature, which is less than or equal to 24° C.

[0010] (3) Volume management: the volume is less than or equal to 40 decibels;

[0011] (4) Lighting management: The lighting time is 10-14 hours in a 24-hour cycle, and the rest is kept without light;

[0012] (5) Density management: The breeding density is less than 80 / m 2 ;

[0013] (6) Feeding management: The feeding frequency is once every 2 days.

[0014] In some embodiments, the depth of the temporary holding container is not less than 25 cm, and the material of the temporary holding container is a light-proof material. The temporary holding container is selected from a blue square plastic box without a lid, a white round freshwater fish breeding box, a household water bucket, a wash basin, etc. commonly used in aquatic products. A glass tank cannot be used because the glass tank is light-transmissive and has poor light-shielding properties.

[0015] In certain embodiments, the room temperature is adjusted by air conditioning.

[0016] In certain embodiments, to ensure that the volume does not exceed 40 decibels, avoid using oxygenation equipment, water pumps and other equipment, and avoid talking loudly.

[0017] In some embodiments, the density of lobster farming does not exceed 70 per meter. 2 .

[0018] In some embodiments, a support is provided in the temporary holding container, and the support is used to filter water, provide ventilation for climbing, and provide a habitat for eating.

[0019] In some embodiments, the support is selected from one or more of volcanic rocks, coral bones, ceramic rings and PVC pipes. Aquatic plants cannot be placed because they are prone to rot and spoil the water quality. The support cannot be placed on the edge or corner to prevent the shrimp from crawling out.

[0020] In certain embodiments, the aquaculture water body is regular tap water, and the aquaculture water body is completely replaced once a week.

[0021] In some embodiments, the water level of the aquaculture water body is 7-10 cm, wherein the shallowest water level does not exceed 8 cm, that is, the water just covers the shrimp back, head and chelipeds to be lifted up to breathe air.

[0022] In certain embodiments, the illumination is outdoor diffuse light or indoor light.

[0023] In certain embodiments, the no-light management method is as follows: the breeding location is not selected at the window door, the windows are curtained, the lights are turned off at night, and the top of the temporary breeding container is covered with a black plastic bag.

[0024] In some embodiments, the feed is fresh feed, and the fresh feed is limited to fresh shrimp tails or small fish. The choice of feed should not be fed with formula feed, which can easily deteriorate the water quality and provide insufficient nutrition. However, if it is used for nutritional experiments, it needs to be prepared separately.

[0025] In certain embodiments, the feed is fed at a rate of 3-4% / shrimp weight, with no food left in the water.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention is different from outdoor breeding of Procambarus clarkii, such as pond breeding, rice field breeding, etc. The present invention proposes an equipment-free indoor temporary breeding method for Procambarus clarkii, especially for simple indoor environmental conditions. It does not require the use of professional aquatic equipment and meticulous management, saving labor; the breeding environment is replicable, and can meet the needs of most conventional scientific experiments for live shrimp sampling; the breeding materials are taken from daily life, with few material restrictions and low costs; the breeding is adapted to local conditions, has strong adaptability, and is easy to promote. Therefore, the present invention can significantly reduce the mortality rate of indoor breeding of Procambarus clarkii, and greatly improve the survival rate, with a 3-month survival rate of more than 80% and a 6-month survival rate of more than 60%, without the need for professional equipment and meticulous system management, and the lack of a wild habitat. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is the survival rate of Procambarus clarkii in Example 4. DETAILED DESCRIPTION

[0029] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0030] The present invention will be further described below in conjunction with the embodiments.

[0031] Example 1

[0032] This example is a sample preparation for the mating experiment of male and female crayfish, which is temporarily raised indoors. The specific scheme is as follows:

[0033] Add aquaculture water to the temporary container, put Procambarus clarkii for aquaculture, and manage it. The specific management items are as follows:

[0034] (1) Twelve small culture boxes of the same size of 40 cm × 30 cm × 25 cm were selected, and one healthy female shrimp and one male shrimp were placed in each culture box for mixed culture.

[0035] (2) Set the water level to about 8 cm.

[0036] (3) The breeding boxes are stacked in a light-proof place. Stacking can increase the degree of light protection. The indoor temperature is adjusted to 24℃ through air conditioning, and the air conditioning is not turned off at night.

[0037] (4) Feed fresh shrimp tails every two days, with the feeding amount being 3% of the shrimp weight.

[0038] (5) Keep quiet, avoid using oxygen equipment, water pumps and other equipment, and avoid talking loudly.

[0039] (6) Completely change the water once a week.

[0040] (7) Observation and recording were performed every day. The experiment lasted for 8 weeks and the survival rate reached 92% (one bird died in each of the two breeding boxes).

[0041] In this embodiment, the selected breeding box is small and the breeding density is very low, so no support is required. The water level is adjusted to about 8 cm according to the situation to facilitate breathing. The experimental requirement of this embodiment is to temporarily culture for 8 weeks. The results show that the survival rate of the temporary culture method of the crayfish of the present invention reaches 92% (as shown in Table 1), which is significantly higher than the survival rate required by the general experiment and meets the experimental requirements.

[0042] Table 1 Grouping and temporary care of this experiment

[0043]

[0044]

[0045] Example 2

[0046] This example is a sample preparation for analyzing the muscle composition of Procambarus clarkii, which is temporarily cultured indoors. The specific scheme is as follows:

[0047] Add aquaculture water to the temporary container, put Procambarus clarkii for aquaculture, and manage it. The specific management items are as follows:

[0048] (1) Three large culture boxes with a size of 50 cm × 40 cm × 40 cm were selected, and 8 female shrimps and 8 male shrimps, a total of 16 young shrimps, were placed in each culture box for mixed culture.

[0049] (2) Place some volcanic rocks and coral bones in the middle of the box, inject tap water, and set the water level to 8 cm.

[0050] (3) The breeding box is placed in a dark place, the indoor temperature is adjusted to 24℃, and the air conditioner is not turned off at night.

[0051] (4) Feed fresh shrimp tails every two days, with the feeding amount being 3% of the shrimp weight.

[0052] (5) Keep quiet, avoid using oxygen equipment, water pumps and other equipment, and avoid talking loudly.

[0053] (6) Completely change the water once a week.

[0054] (7) Observe and record daily, and remove dead shrimp in time.

[0055] (8) Samples were collected from each of the three groups every 4 weeks for analysis. The remaining live shrimp were kept in temporary storage.

[0056] (9) The experiment lasted for 12 weeks, and the terminal survival rates reached 94%, 87.5%, and 81%, respectively.

[0057] In this embodiment, the number of temporary culture containers selected is small, but the volume is large, and the culture density is average, so supports need to be placed. The experimental requirement of this embodiment is to temporarily culture the crayfish indoors for 12 weeks. The results show that the survival rate of the temporary culture method of Procambarus clarkii in this embodiment reaches more than 80% at the end, which can meet the experimental requirements and provide a solid foundation for the development of the experiment and the analysis of the results.

[0058] Example 3

[0059] This embodiment provides a method for temporarily culturing Procambarus clarkii indoors without equipment, and the specific technical solution is as follows:

[0060] (1) A large culture box with a size of 63 cm × 46 cm × 35 cm was selected, and 18 Procambarus clarkii crayfish were placed in the culture box.

[0061] (2) Place some volcanic rocks in the middle of the box to ensure that the breeding space is larger than 30 cm × 30 cm, inject tap water, and set the water level to 8 cm.

[0062] (3) The breeding box is placed in a dark place, the indoor temperature is adjusted to 24℃, and the air conditioner is not turned off at night.

[0063] (4) Feed fresh shrimp tails every two days, with the feeding amount being 3% of the shrimp weight.

[0064] (5) Keep quiet, avoid using oxygen equipment, water pumps and other equipment, and avoid talking loudly.

[0065] (6) Completely change the water once a week.

[0066] (7) Observe and record daily, and remove dead shrimp in time.

[0067] Table 2 Correlation comparison between the effects of the present invention and the effects of the control group

[0068]

[0069] *No measures means no management is taken; the control group is the patent application with publication number CN111700012A; the management items include water management, temperature management, volume management, light management, density management, feeding management and whether to set up supports.

[0070] As shown in Table 2, the 3-month survival rate of Procambarus clarkii in this embodiment exceeded 80%, and the 6-month survival rate exceeded 60%. Although lower than that of the control group, this embodiment does not have oxygenation equipment and water pumps, and thus has low energy consumption costs and is highly applicable.

[0071] Example 4

[0072] This embodiment provides a method for temporarily culturing Procambarus clarkii indoors without equipment, and the specific technical solution is as follows:

[0073] Add aquaculture water to the temporary container, put Procambarus clarkii for aquaculture, and manage it. The specific management items are as follows:

[0074] (1) A large culture box with a size of 60 cm × 50 cm × 40 cm was selected, and 21 Procambarus clarkii were placed in the culture box.

[0075] (2) Place some volcanic rocks in the middle of the box to ensure that the breeding space is larger than 30 cm × 30 cm, inject tap water, and set the water level to 8 cm.

[0076] (3) The breeding box is placed in a dark place, the indoor temperature is adjusted to 24℃, and the air conditioner is not turned off at night.

[0077] (4) Feed fresh shrimp tails every two days, with the feeding amount being 3% of the shrimp weight.

[0078] (5) Keep quiet, avoid using oxygen equipment, water pumps and other equipment, and avoid talking loudly.

[0079] (6) Completely change the water once a week.

[0080] (7) Observe and record daily, and remove dead shrimp in time.

[0081] like Figure 1 As shown, in this embodiment, the 3-month survival rate of Procambarus clarkii exceeds 80%, and the 6-month survival rate is close to 70%.

[0082] In summary, the present invention specifically targets the physiological characteristics of Procambarus clarkii and the requirements of laboratory work, avoids the use of professional equipment, and creates a simple-to-operate and replicable breeding environment under relatively simple indoor conditions, thereby maintaining the life characteristics and quality of life of the living Procambarus clarkii.

[0083] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. The present invention is not limited to the above examples. Any changes, modifications, additions or substitutions made by technicians in this technical field within the essential scope of the present invention should also fall within the protection scope of the present invention.

Claims

1. A method for temporarily culturing Procambarus clarkii indoors without equipment, characterized in that: The method comprises adding aquaculture water into a temporary container, placing Procambarus clarkii in the container for aquaculture, and performing management; the aquaculture management comprises the following contents: (1) Water management: The water depth of the aquaculture water body shall not exceed 10 cm; (2) Temperature management: the water temperature is adjusted by room temperature, which is less than or equal to 24° C. (3) Volume management: the volume is less than or equal to 40 decibels; (4) Lighting management: The lighting time is 10-14 hours in a 24-hour cycle, and the rest is kept without light; (5) Density management: The breeding density is less than 80 / m 2 ; (6) Feeding management: The feeding frequency is once every 2 days.

2. The method according to claim 1, characterized in that The depth of the temporary holding container is not less than 25 cm, and the material of the temporary holding container is a light-proof material.

3. The method according to claim 1 or 2, characterized in that: The breeding density shall not exceed 70 per meter. 2 .

4. The method according to any one of claims 1 to 3, characterized in that: The temporary storage container is provided with a support, and the support is selected from one or more of volcanic rocks, coral bones, ceramic rings and PVC pipes.

5. The method according to any one of claims 1 to 4, characterized in that: Conventional tap water is selected as the aquaculture water body, and the aquaculture water body is completely replaced once a week.

6. The method according to any one of claims 1 to 5, characterized in that: The water depth of the aquaculture water body is 7-10 cm.

7. The method according to any one of claims 1 to 6, characterized in that: The illumination adopts outdoor scattered light or indoor light.

8. The method according to any one of claims 1 to 7, characterized in that: The no-light management method is as follows: avoid raising the animals near windows or doorways, draw curtains on windows, turn off lights at night, and cover the tops of temporary containers with black plastic bags.

9. The method according to any one of claims 1 to 8, characterized in that: The feed is fresh feed, and the fresh feed is fresh shrimp tails or small fish.

10. The method according to any one of claims 1 to 8, characterized in that: The feeding amount is 3-4% of the weight of the crayfish.

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