Method for enabling grease of tenebrio molitor to be rich in natural vitamin E by feeding citral

Abstract

The invention belongs to the technical field of breeding, and particularly relates to a method for enabling grease of tenebrio molitor to be rich in natural vitamin E by feeding citral. The method comprises the following steps: S1, selecting healthy and active tenebrio molitor larvae as experimental subjects; s2, mixing citral with a feed to obtain a feed containing citral; s3, mixing the citral-containing feed and the citral-free feed to obtain feeding feed, and feeding the tenebrio molitor larvae with the feeding feed; and S4, extracting and collecting the grease of the adult tenebrio molitor to obtain the natural vitamin E-rich tenebrio molitor grease. According to the method for feeding the tenebrio molitor, the content of vitamin E in the obtained tenebrio molitor grease can be effectively increased, then the stability of the tenebrio molitor grease in the storage process is effectively improved, and the shelf life of the tenebrio molitor grease is greatly prolonged.

Description

Technical Field

[0001] The invention belongs to the technical field of breeding, and particularly relates to a method for enriching yellow mealworm oil with natural vitamin E by feeding citral. Background Art

[0002] Mealworm is a common insect, and its oil is rich in unsaturated fatty acids, has high economic value, and can be used in many fields. However, mealworm oil is easily oxidized during storage, producing peroxides, increasing acid value, and causing its stability to decrease. In order to extend the shelf life of mealworm oil, antioxidants are usually added, but in some application fields of insect oil, there are strict restrictions on exogenously added antioxidants. Therefore, it is urgent to develop a method that can effectively improve the stability of mealworm oil. Summary of the invention

[0003] The present invention aims to provide a method for enriching yellow mealworm oil with natural vitamin E by feeding citral. The yellow mealworm feeding method of the present invention can effectively increase the content of natural vitamin E in the obtained yellow mealworm oil, thereby effectively improving the stability of the yellow mealworm oil during storage, and greatly extending the shelf life of the yellow mealworm oil.

[0004] In order to achieve the above object, the present invention adopts the following technical scheme: a method for enriching yellow mealworm oil with natural vitamin E by feeding citral, comprising the following steps:

[0005] S1. Select healthy and active mealworm larvae as experimental subjects;

[0006] S2, mixing citral with feed to obtain feed containing citral;

[0007] S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae;

[0008] S4, extracting and collecting the oil of the mealworm adults to obtain the mealworm oil rich in natural vitamin E.

[0009] Preferably, the proportion of citral in the feeding feed in step S3 is adjusted according to the different growth stages of the mealworm larvae.

[0010] Preferably, the different growth stages of the mealworm larvae include 20 days old, 20 to 50 days old and after 50 days old.

[0011] Preferably, in step S3, the proportion of citral-containing feed added to the yellow mealworm larvae at different growth stages of 20 days old, 20 to 50 days old, and after 50 days old is 8 to 11 wt.%, 13 to 16 wt.%, and 18 to 23 wt.% of the total mass of the feeding feed, respectively.

[0012] More preferably, in step S3, the proportion of citral-containing feed added to the yellow mealworm larvae at different growth stages of 20 days old, 20 to 50 days old, and after 50 days old is 10wt.%, 15wt.%, and 20wt.% of the total mass of the feeding feed, respectively.

[0013] Preferably, the method for preparing the citral-containing feed in step S2 comprises the following steps: mixing citral with water, adding an emulsifier, stirring and emulsifying to form a stable suspension, and mixing with mealworm feed to obtain the citral-containing feed.

[0014] Preferably, in the method for preparing a feed containing citral, the mass ratio of citral to water is 1:(20-50).

[0015] Preferably, in the method for preparing the citral-containing feed, the amount of citral added is 1-5 kg / ton of mealworm feed.

[0016] Preferably, in the method for preparing a feed containing citral, the content of the emulsifier is 3 to 10% of the total mass of the feed containing citral.

[0017] The emulsifier added in the preparation method of the feed containing citral of the present invention can be selected from conventional emulsifier components in the art such as gum arabic and Tween 80.

[0018] Preferably, the method for extracting and collecting the fat of the adult mealworms in step S4 is supercritical carbon dioxide extraction.

[0019] The present invention also claims protection for the yellow mealworm oil prepared by the method of enriching yellow mealworm oil with natural vitamin E by feeding citral. The content of vitamin E in the prepared yellow mealworm oil can reach more than 250 mg / 100 g, and can reach up to 900 mg / 100 g.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention feeds citral to the mealworms, so that the mealworm oil is naturally rich in natural vitamin E, thereby improving the stability and nutritional value of the mealworm oil. At the same time, the dependence on artificially added antioxidants is reduced, and the production cost and environmental pollution risk are reduced. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] In the embodiments and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0024] Example 1

[0025] A method for enriching mealworm oil with natural vitamin E by feeding citral, comprising the following steps:

[0026] S1. Select healthy and active mealworm larvae as experimental subjects;

[0027] S2, mixing citral with feed to obtain feed containing citral;

[0028] S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae;

[0029] S4. The supercritical carbon dioxide extraction method is used to extract and collect the oil of the yellow mealworm adults to obtain the yellow mealworm oil rich in natural vitamin E.

[0030] The method for preparing the citral-containing feed comprises the following steps: mixing citral with water (the mass ratio of citral to water is 1:30), adding an emulsifier, stirring and emulsifying to form a stable suspension, and mixing it with mealworm feed (the content of citral is 3.2 kg / ton of mealworm feed) to obtain the citral-containing feed.

[0031] Wherein, the ratio of the feed containing citral to the feed not containing citral in step S3 is adjusted according to the following growth stages of the mealworm larvae:

[0032] 20 days old: citral-containing feed accounts for 10% of the total weight of the feed;

[0033] 20-50 days old: citral-containing feed accounts for 15% of the total weight of the feed;

[0034] After 50 days of age: citral-containing feed accounts for 20% of the total mass of the feed.

[0035] Example 2

[0036] A method for enriching mealworm oil with natural vitamin E by feeding citral, comprising the following steps:

[0037] S1. Select healthy and active mealworm larvae as experimental subjects;

[0038] S2, mixing citral with feed to obtain feed containing citral;

[0039] S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae;

[0040] S4. The supercritical carbon dioxide extraction method is used to extract and collect the oil of the yellow mealworm adults to obtain the yellow mealworm oil rich in natural vitamin E.

[0041] The preparation method of the citral-containing feed comprises the following steps: mixing citral with water (the mass ratio of citral to water is 1:20), adding an emulsifier, stirring and emulsifying to form a stable suspension, and mixing it with mealworm feed (the content of citral is 1kg / ton of mealworm feed) to obtain the citral-containing feed.

[0042] Wherein, the ratio of the feed containing citral to the feed not containing citral in step S3 is adjusted according to the following growth stages of the mealworm larvae:

[0043] 20 days old: citral-containing feed accounts for 8% of the total weight of the feed;

[0044] 20-50 days old: citral-containing feed accounts for 13% of the total weight of the feed;

[0045] After 50 days of age: feed containing citral accounts for 18% of the total weight of the fed feed.

[0046] Example 3

[0047] A method for enriching mealworm oil with natural vitamin E by feeding citral, comprising the following steps:

[0048] S1. Select healthy and active mealworm larvae as experimental subjects;

[0049] S2, mixing citral with feed to obtain feed containing citral;

[0050] S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae;

[0051] S4. The supercritical carbon dioxide extraction method is used to extract and collect the oil of the yellow mealworm adults to obtain the yellow mealworm oil rich in natural vitamin E.

[0052] The preparation method of the citral-containing feed comprises the following steps: mixing citral with water (the mass ratio of citral to water is 1:50), adding an emulsifier, stirring and emulsifying to form a stable suspension, and mixing it with mealworm feed (the content of citral is 5kg / ton of mealworm feed) to obtain the citral-containing feed.

[0053] Wherein, the ratio of the feed containing citral to the feed not containing citral in step S3 is adjusted according to the following growth stages of the mealworm larvae:

[0054] 20 days old: citral-containing feed accounts for 10% of the total weight of the feed;

[0055] 20-50 days old: citral-containing feed accounts for 15% of the total weight of the feed;

[0056] After 50 days of age: feed containing citral accounts for 23% of the total mass of the fed feed.

[0057] Example 4

[0058] A method for enriching mealworm oil with natural vitamin E by feeding citral, comprising the following steps:

[0059] S1. Select healthy and active mealworm larvae as experimental subjects;

[0060] S2, mixing citral with feed to obtain feed containing citral;

[0061] S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae;

[0062] S4. The supercritical carbon dioxide extraction method is used to extract and collect the oil of the yellow mealworm adults to obtain the yellow mealworm oil rich in natural vitamin E.

[0063] The method for preparing the citral-containing feed comprises the following steps: mixing citral with water (the mass ratio of citral to water is 1:30), adding an emulsifier, stirring and emulsifying to form a stable suspension, and mixing it with mealworm feed (the content of citral is 3.2 kg / ton of mealworm feed) to obtain the citral-containing feed.

[0064] Wherein, the ratio of the feed containing citral to the feed not containing citral in step S3 is adjusted according to the following growth stages of the mealworm larvae:

[0065] 20 days old: citral-containing feed accounts for 15% of the total weight of the feed;

[0066] 20-50 days old: citral-containing feed accounts for 20% of the total weight of the feed;

[0067] After 50 days of age: feed containing citral accounts for 10% of the total mass of the feed.

[0068] Example 5

[0069] A method for enriching mealworm oil with natural vitamin E by feeding citral, comprising the following steps:

[0070] S1. Select healthy and active mealworm larvae as experimental subjects;

[0071] S2, mixing citral with feed to obtain feed containing citral;

[0072] S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae;

[0073] S4. The supercritical carbon dioxide extraction method is used to extract and collect the oil of the yellow mealworm adults to obtain the yellow mealworm oil rich in natural vitamin E.

[0074] The method for preparing the citral-containing feed comprises the following steps: mixing citral with water (the mass ratio of citral to water is 1:30), adding an emulsifier, stirring and emulsifying to form a stable suspension, and mixing it with mealworm feed (the content of citral is 3.2 kg / ton of mealworm feed) to obtain the citral-containing feed.

[0075] Wherein, the ratio of the feed containing citral to the feed not containing citral in step S3 is adjusted according to the following growth stages of the mealworm larvae:

[0076] 20 days old: citral-containing feed accounts for 20% of the total weight of the feed;

[0077] 20-50 days old: citral-containing feed accounts for 20% of the total weight of the feed;

[0078] After 50 days of age: citral-containing feed accounts for 20% of the total mass of the feed.

[0079] Example 6

[0080] A method for enriching mealworm oil with natural vitamin E by feeding citral, comprising the following steps:

[0081] S1. Select healthy and active mealworm larvae as experimental subjects;

[0082] S2, mixing citral with feed to obtain feed containing citral;

[0083] S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae;

[0084] S4. The supercritical carbon dioxide extraction method is used to extract and collect the oil of the yellow mealworm adults to obtain the yellow mealworm oil rich in natural vitamin E.

[0085] The method for preparing the citral-containing feed comprises the following steps: mixing citral with water (the mass ratio of citral to water is 1:30), adding an emulsifier, stirring and emulsifying to form a stable suspension, and mixing it with mealworm feed (the content of citral is 6kg / ton of mealworm feed) to obtain the citral-containing feed.

[0086] Wherein, the ratio of the feed containing citral to the feed not containing citral in step S3 is adjusted according to the following growth stages of the mealworm larvae:

[0087] 20 days old: citral-containing feed accounts for 10% of the total weight of the feed;

[0088] 20-50 days old: citral-containing feed accounts for 15% of the total weight of the feed;

[0089] After 50 days of age: citral-containing feed accounts for 20% of the total mass of the feed.

[0090] Test Example 1

[0091] The yellow mealworm oil extracted in the example was tested for the average value of the vitamin E content in the yellow mealworm oil according to "Feed Additive Vitamin E (GB / T 9454-2008)". The test results are shown in Table 1.

[0092] The content of vitamin E in peanut oil currently on the market is about 42 mg / 100 g.

[0093] Table 1

[0094]

[0095] It can be seen from the experimental data in Table 1 that citral is used to feed mealworms in the embodiment of the present invention, and the oil finally extracted from the mealworms contains a high content of vitamin E, which can be achieved at more than 250 mg / 100 g, which is much higher than the vitamin E content of about 42 mg / 100 g contained in peanut oil in the existing market.

[0096] Compared with Example 1, in Example 4, the citral content fed to the yellow mealworms at different growth stages of 20 days old, 20 to 50 days old, and after 50 days old was different, and the vitamin E content contained in the yellow mealworm oil finally extracted was reduced;

[0097] Compared with Example 1, in Example 5, the content of citral in the feed fed to the yellow mealworms at different growth stages of 20 days, 20 to 50 days, and after 50 days of age was 20% of the total mass of the feed, and the vitamin E content in the yellow mealworm oil finally extracted was lower than that in the example;

[0098] Compared with Example 1, in Example 6, the content of citral added during the preparation of the citral-containing feed is 6 kg / ton of mealworm feed, which reduces the content of vitamin E contained in the oil extracted from mealworms fed at different feeding times.

[0099] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for enriching mealworm oil with natural vitamin E by feeding citral, characterized in that: The following steps are involved: S1. Select healthy and active mealworm larvae as experimental subjects; S2, mixing citral with feed to obtain feed containing citral; S3, mixing the feed containing citral and the feed not containing citral to obtain a feeding feed, and feeding the feed to the mealworm larvae; S4, extracting and collecting the oil of the mealworm adults to obtain the mealworm oil rich in natural vitamin E.

2. A method for enriching mealworm oil with natural vitamin E by feeding citral as claimed in claim 1, characterized in that: The proportion of citral in the feeding feed in step S3 is adjusted according to the different growth stages of the mealworm larvae.

3. A method for enriching mealworm oil with natural vitamin E by feeding citral as claimed in claim 2, characterized in that: The different growth stages of the yellow mealworm larvae include 20 days old, 20 to 50 days old and after 50 days old.

4. A method for enriching mealworm oil with natural vitamin E by feeding citral as claimed in claim 3, characterized in that: In step S3, the proportion of citral-containing feed added to the yellow mealworm larvae at different growth stages of 20 days old, 20 to 50 days old, and after 50 days old is 8 to 11 wt.%, 13 to 16 wt.%, and 18 to 23 wt.% of the total mass of the feeding feed, respectively.

5. A method for enriching mealworm oil with natural vitamin E by feeding citral as claimed in claim 4, characterized in that: In step S3, the proportion of citral-containing feed added to the mealworm larvae at different growth stages of 20 days old, 20 to 50 days old, and after 50 days old is 10wt.%, 15wt.%, and 20wt.% of the total mass of the feeding feed, respectively.

6. A method for enriching mealworm oil with natural vitamin E by feeding citral as claimed in claim 1, characterized in that: The method for preparing the citral-containing feed in step S2 comprises the following steps: mixing citral with water, adding an emulsifier, stirring, and mixing with yellow mealworm feed to obtain the citral-containing feed.

7. A method for enriching mealworm oil with natural vitamin E by feeding citral as claimed in claim 6, characterized in that: At least one of the following (1) to (3): (1) The mass ratio of citral to water is 1:(20-50); (2) The amount of citral added is 1-5 kg / ton of mealworm feed; (3) The content of the emulsifier is 3 to 10% of the total mass of the feed containing citral.

8. A method for enriching mealworm oil with natural vitamin E by feeding citral as claimed in claim 1, characterized in that: The method for extracting and collecting the fat of the mealworm adults in step S4 is supercritical carbon dioxide extraction.

9. A mealworm oil prepared by the method of enriching mealworm oil with natural vitamin E by feeding citral as claimed in any one of claims 1 to 8.

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