Biological bacteriostatic agent

Abstract

The invention discloses a biological bacteriostatic agent, which belongs to the technical field of biological bacteriostatic agents and comprises the following components: gaseous available chlorine, creeping oxalis extract, sericin and trehalose. The agricultural product sterilization and preservation device can sterilize agricultural products, can preserve the agricultural products, and improves the taste and nutrition of the agricultural products; meanwhile, no toxic effect is generated, and cleaning residues cannot cause harm to human health after being eaten; meanwhile, the preparation processes of the gaseous available chlorine, the oxalis corniculata extracting solution and the sericin complement each other, that is, the product of the gaseous available chlorine can be used for preparing a biological bacteriostatic agent and the oxalis corniculata extracting solution, and heat produced by the oxalis corniculata extracting solution can be used for preparing the sericin, so that the preparation cost can be reduced to the greatest extent; the preparation yield is improved, and the method has wide market competitiveness.

Description

Technical Field

[0001] The invention belongs to the technical field of biological bacteriostatic agents, and specifically relates to a biological bacteriostatic agent. Background Art

[0002] If agricultural products are not properly preserved after being picked or harvested, they are easily contaminated by microorganisms and cause them to rot and deteriorate. Not only will the taste and nutritional value be greatly reduced, but harmful substances may also be produced, posing a threat to human health.

[0003] The current method of preserving agricultural products is generally to spray preservatives and other chemical products to inhibit microbial and enzyme activities. This method has the following problems: the chemical agents may not be completely removed during the agricultural product cleaning stage and will remain on the surface of the agricultural products, which may affect human health after consumption.

[0004] In view of this, a biological antibacterial agent is designed to solve the above problems. Summary of the invention

[0005] To solve the problems raised in the above background technology. The present invention provides a biological antibacterial agent, which can not only sterilize agricultural products, but also preserve agricultural products, improve the taste and nutrition of agricultural products, and has no toxic effects. The cleaning residue will not cause harm to human health after being eaten. At the same time, it can reduce the expenditure of preparation cost to the greatest extent, improve the preparation yield, and has the characteristics of broad market competitiveness.

[0006] To achieve the above object, the present invention provides the following technical solution: a biological antibacterial agent, comprising the following components:

[0007] Gaseous available chlorine, Oxalis extract, sericin and trehalose;

[0008] Gaseous available chlorine is obtained by injecting saturated salt water into an electrolytic cell for electrolysis to generate sodium hydroxide solution, chlorine gas and hydrogen gas. The sodium hydroxide solution, chlorine gas and hydrogen gas are separated and purified to obtain chlorine gas, which is gaseous available chlorine.

[0009] The Oxalis extract is prepared by reacting carbon monoxide with separated and purified hydrogen to generate methanol, fresh Oxalis is picked, washed, dried, and crushed, the crushed Oxalis residue and the generated methanol are placed in a solvent container, the Oxalis residue is immersed in methanol, the solvent container is heated, the Oxalis residue and methanol are heated, the methanol evaporates to form steam, the steam rises to a condenser, cools and refluxes into the solvent container until the heating time is up, the heating is stopped, the extract in the solvent container is filtered, and a methanol extract of quercetin is obtained, and the quercetin is separated and purified by adsorption with DM-130 adsorption resin to obtain the quercetin, that is, the Oxalis extract;

[0010] Sericin is prepared by cutting high-quality silkworm cocoons into small pieces with scissors, washing with deionized water to remove surface impurities, mixing with separated and purified sodium hydroxide solution, preheating with waste heat from a condenser tube and then reheating with a heater, stirring and mixing, waiting for the silkworm cocoons to dissolve in the sodium hydroxide solution until they are completely dissolved, filtering the mixture to remove insoluble impurities, centrifuging to separate the sericin solution, and freeze-drying to remove moisture from the sericin solution;

[0011] Trehalose is prepared by inoculating lactobacillus onto a slant medium for activation culture, then inoculating it into a shake flask medium for shake flask culture to allow the strain to reach a logarithmic growth phase, then inoculating it into a fermenter for fermentation, and then centrifuging and purifying it.

[0012] The method for preparing a biological antibacterial agent comprises the following steps:

[0013] S1: Pass gaseous available chlorine into separated and purified sodium hydroxide solution or pure water to adjust the pH value until the pH value reaches 8.5-9.5;

[0014] S2: Place Oxalis sorrel extract, sericin and trehalose in a volume ratio of 1:1 with gaseous available chlorine into the above solution, stir and mix until the mixture is uniform, and prepare a biological antibacterial agent.

[0015] Furthermore, in step S1, the specific steps of gaseous available chlorine include:

[0016] S101: Design and build an electrolytic cell with adjustable current, voltage and temperature parameters. Install anodes and cathodes in the electrolytic cell, and connect the anodes and cathodes to an external power source through wires;

[0017] S102: Inject saturated salt water into the electrolytic cell and turn on the power supply. The current flows between the anode and the cathode, triggering an electrolytic reaction. An oxidation reaction occurs at the anode and a reduction reaction occurs at the cathode, generating sodium hydroxide solution, chlorine gas and hydrogen gas. The chlorine gas is the gaseous available chlorine.

[0018] Furthermore, in step S1, the sodium hydroxide solution is the sodium hydroxide solution separated and purified in step S102.

[0019] Furthermore, in step S2, the specific steps of extracting Oxalis sorrel include:

[0020] S201: constructing a reaction system structure, including a solvent container A, a heater A, a condenser and a cooler, wherein the solvent container A is placed in the heater A, the condenser is placed above the solvent container A and fully covers the opening of the solvent container A, the cooler covers the condenser, and the condenser is located above the solvent container A;

[0021] S202: picking fresh Oxalis, washing, drying, and placing in a crushing container for crushing;

[0022] S203: reacting carbon monoxide with the hydrogen separated and purified in step S102 to generate methanol;

[0023] S204: placing the crushed Oxalis residue and methanol in a solvent container A, so that the methanol submerges the Oxalis residue;

[0024] S205: Heater A heats the Oxalis residue and methanol. During the heating process, methanol evaporates to form steam. The steam rises to the condenser. Under the action of the cooler providing a cold source, the steam is cooled into liquid in the condenser and flows back into the solvent container A.

[0025] S206: When the set reaction time is reached, the heating is stopped, and the extract in the solvent container A is placed in a filter container to filter out the residue to obtain a methanol extract of quercetin;

[0026] S207: quercetin, i.e., Oxalis sorrel extract, is separated and purified by adsorption using DM-130 adsorption resin.

[0027] Furthermore, in step S2, the specific steps of preparing sericin include:

[0028] S208: constructing a reaction system structure, including a solvent container B and a heater B, wherein the condenser in step S201 is wound around the outer wall of the solvent container B, the cooler is placed at the end of the condenser, and the solvent container B is placed in the heater B;

[0029] S209: Prepare the finest silkworm cocoons;

[0030] S210: Cut the cocoons into small pieces using scissors;

[0031] S211: Clean the small pieces of silk cocoons with deionized water to remove surface impurities;

[0032] S212: placing the cleaned small pieces of silk cocoons in a solvent container B, adding the sodium hydroxide solution separated and purified in step S102, first preheating them with the residual heat of the steam in the wound condenser tube, and then heating them with the heater B, stirring and mixing during the heating process, and waiting for the silk cocoons to dissolve in the sodium hydroxide solution;

[0033] S213: After being completely dissolved, the mixture is placed in a filtering container to filter out insoluble impurities;

[0034] S214: placing the filtrate in a centrifugal processor for centrifugal treatment to separate the sericin solution;

[0035] S215: placing the sericin solution in a freeze dryer for freeze drying to remove moisture in the sericin solution and obtain sericin protein.

[0036] Furthermore, in step S2, the specific steps of preparing trehalose include:

[0037] S216: inoculating lactobacillus onto the slant culture medium for activation culture;

[0038] S217: inoculating the activated strain from the slant culture medium into the shake flask culture medium, and culturing the strain in the shake flask until the strain reaches the logarithmic growth phase;

[0039] S218: inoculating the bacterial liquid obtained by the shake flask culture into a fermentation tank for fermentation;

[0040] S219: After the fermentation is completed, the fermentation liquid is placed in a centrifuge for centrifugation to obtain a fermentation liquid;

[0041] S220: pumping the fermentation liquid into the ion exchange column, the trehalose in the fermentation liquid is exchanged with the ions on the ion exchange resin in the ion exchange column, the ion exchange column is eluted with the eluent, the trehalose is eluted from the ion exchange resin, and the eluent, i.e., the trehalose solution, is collected.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] The invention is prepared from gaseous available chlorine, Oxalis extract, sericin and trehalose, wherein the gaseous available chlorine has a disinfecting effect, the Oxalis extract has an antioxidant effect, and the sericin has an antioxidant effect. At the same time, a hydration film can be formed after spraying, which has the effects of moisturizing, preserving and forming a natural barrier to inhibit pests and diseases. The trehalose prepared by microorganisms has the effects of retaining water and improving nutrition. The combination of the four can not only sterilize agricultural products, but also preserve agricultural products, improve the taste and nutrition of agricultural products; at the same time, it has no toxic effect, and the cleaning residue will not cause harm to human health after being eaten; at the same time, the preparation processes of gaseous available chlorine, Oxalis extract and sericin complement each other, that is, the product of gaseous available chlorine can be used for the preparation of biological antibacterial agents and the preparation of Oxalis extract, and the heat generated by the Oxalis extract can be used for the preparation of sericin, which can reduce the expenditure of preparation cost to the greatest extent, improve the preparation yield, and has wide market competitiveness. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 The present invention is a flow chart of the method for preparing the biological antibacterial agent. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] A biological antibacterial agent, comprising the following components:

[0047] Gaseous available chlorine, Oxalis extract, sericin and trehalose;

[0048] Gaseous available chlorine is obtained by injecting saturated salt water into an electrolytic cell for electrolysis to generate sodium hydroxide solution, chlorine gas and hydrogen gas. The sodium hydroxide solution, chlorine gas and hydrogen gas are separated and purified to obtain chlorine gas, which is gaseous available chlorine.

[0049] The Oxalis extract is prepared by reacting carbon monoxide with separated and purified hydrogen to generate methanol, fresh Oxalis is picked, washed, dried, and crushed, the crushed Oxalis residue and the generated methanol are placed in a solvent container, the Oxalis residue is immersed in methanol, the solvent container is heated, the Oxalis residue and methanol are heated, the methanol evaporates to form steam, the steam rises to a condenser, cools and refluxes into the solvent container until the heating time is up, the heating is stopped, the extract in the solvent container is filtered, and a methanol extract of quercetin is obtained, and the quercetin is separated and purified by adsorption with DM-130 adsorption resin to obtain the quercetin, that is, the Oxalis extract;

[0050] Sericin is prepared by cutting high-quality silkworm cocoons into small pieces with scissors, washing with deionized water to remove surface impurities, mixing with separated and purified sodium hydroxide solution, preheating with waste heat from a condenser tube and then reheating with a heater, stirring and mixing, waiting for the silkworm cocoons to dissolve in the sodium hydroxide solution until they are completely dissolved, filtering the mixture to remove insoluble impurities, centrifuging to separate the sericin solution, and freeze-drying to remove moisture from the sericin solution;

[0051] Trehalose is prepared by inoculating lactobacillus onto a slant medium for activation culture, then inoculating it into a shake flask medium for shake flask culture to allow the strain to reach a logarithmic growth phase, then inoculating it into a fermenter for fermentation, and then centrifuging and purifying it.

[0052] See attached Figure 1 , a method for preparing a biological antibacterial agent, comprising the following steps:

[0053] S1: Pass gaseous available chlorine into separated and purified sodium hydroxide solution or pure water to adjust the pH value until the pH value reaches 8.5-9.5;

[0054] In this embodiment, the addition ratio of gaseous available chlorine to sodium hydroxide solution or purified water is to adjust the pH value of the mixed solution to 8.5-9.5;

[0055] S2: Place Oxalis sorrel extract, sericin and trehalose in a volume ratio of 1:1 with gaseous available chlorine into the above solution, stir and mix until the mixture is uniform, and prepare a biological antibacterial agent.

[0056] Specifically, in step S1, the specific steps of gaseous available chlorine include:

[0057] S101: Design and build an electrolytic cell with adjustable current, voltage and temperature parameters. Install anodes and cathodes in the electrolytic cell, and connect the anodes and cathodes to an external power source through wires;

[0058] S102: Inject saturated salt water into the electrolytic cell and turn on the power supply. The current flows between the anode and the cathode, triggering an electrolytic reaction. An oxidation reaction occurs at the anode and a reduction reaction occurs at the cathode, generating sodium hydroxide solution, chlorine gas and hydrogen gas. The chlorine gas is the gaseous available chlorine.

[0059] In this embodiment, the chemical reaction equation is as follows:

[0060] 2NaCl+2H2O=2NaOH+Cl2↑+H2↑

[0061] The addition ratio of NaCl and H2O in the saturated salt water satisfies the completion of the above chemical reaction equation;

[0062] Gaseous available chlorine can quickly inhibit a variety of bacteria such as Escherichia coli, Staphylococcus aureus and Candida albicans, as well as bacterial silkworm disease pathogens such as Bombyx mori nuclear polyhedrosis virus, cytoplasmic polyhedrosis virus, densorrhea virus and malarial disease virus.

[0063] Specifically, in step S1, the sodium hydroxide solution is the sodium hydroxide solution separated and purified in step S102.

[0064] Specifically, in step S2, the specific steps of extracting Oxalis Sorrel include:

[0065] S201: constructing a reaction system structure, including a solvent container A, a heater A, a condenser and a cooler, wherein the solvent container A is placed in the heater A, the condenser is placed above the solvent container A and fully covers the opening of the solvent container A, the cooler covers the condenser, and the condenser is located above the solvent container A;

[0066] S202: picking fresh Oxalis, washing, drying, and placing in a crushing container for crushing;

[0067] S203: reacting carbon monoxide with the hydrogen separated and purified in step S102 to generate methanol;

[0068] In this embodiment, the chemical reaction equation is as follows:

[0069] CO↑+2H2↑=CH3OH

[0070] In this embodiment, the addition ratio of CO and H2 satisfies the above chemical reaction equation;

[0071] S204: placing the crushed Oxalis residue and methanol in a solvent container A, so that the methanol submerges the Oxalis residue;

[0072] S205: Heater A heats the Oxalis residue and methanol. During the heating process, methanol evaporates to form steam. The steam rises to the condenser. Under the action of the cooler providing a cold source, the steam is cooled into liquid in the condenser and flows back into the solvent container A.

[0073] S206: When the set reaction time is reached, the heating is stopped, and the extract in the solvent container A is placed in a filter container to filter out the residue to obtain a methanol extract of quercetin;

[0074] S207: quercetin, i.e., Oxalis sorrel extract, is separated and purified by adsorption using DM-130 adsorption resin.

[0075] Quercetin has strong antioxidant and antibacterial effects. Spraying it on the surface of agricultural products can extend their storage period, reduce the weight loss rate and pathology rate of agricultural products during storage, and slow down the decline in soluble solids, total acid and total sugar content. It protects VC, maintains the activity of CAT and SOD, reduces POD activity, and delays the aging of fruit tissue.

[0076] Specifically, in step S2, the specific steps of sericin include:

[0077] S208: constructing a reaction system structure, including a solvent container B and a heater B, wherein the condenser in step S201 is wound around the outer wall of the solvent container B, the cooler is placed at the end of the condenser, and the solvent container B is placed in the heater B;

[0078] S209: Prepare the finest silkworm cocoons;

[0079] S210: Cut the cocoons into small pieces using scissors;

[0080] S211: Clean the small pieces of silk cocoons with deionized water to remove surface impurities;

[0081] S212: placing the cleaned small pieces of silk cocoons in a solvent container B, adding the sodium hydroxide solution separated and purified in step S102, first preheating them with the residual heat of the steam in the wound condenser tube, and then heating them with the heater B, stirring and mixing during the heating process, and waiting for the silk cocoons to dissolve in the sodium hydroxide solution;

[0082] S213: After being completely dissolved, the mixture is placed in a filtering container to filter out insoluble impurities;

[0083] S214: placing the filtrate in a centrifugal processor for centrifugal treatment to separate the sericin solution;

[0084] S215: placing the sericin solution in a freeze dryer for freeze drying to remove moisture in the sericin solution and obtain sericin protein.

[0085] Sericin has strong antioxidant and dismutase properties, which can prevent the attack of active oxygen produced during metabolism and effectively maintain the integrity of the membrane structure. It also has the function of absorbing water and retaining moisture. When its aqueous solution is sprayed on the surface of agricultural products, it can form a layer of hydration film. This film is like a natural barrier that can effectively inhibit the occurrence of plant diseases and play a role in preserving freshness.

[0086] Specifically, in step S2, the specific steps of preparing trehalose include:

[0087] S216: inoculating lactobacillus onto the slant culture medium for activation culture;

[0088] S217: inoculating the activated strain from the slant culture medium into the shake flask culture medium, and culturing the strain in the shake flask until the strain reaches the logarithmic growth phase;

[0089] In this embodiment, the shaking flask culture temperature is 30°C, the shaking flask culture speed is 140r / min, and the shaking flask culture time is 20h;

[0090] S218: inoculating the bacterial liquid obtained by the shake flask culture into a fermentation tank for fermentation;

[0091] In this embodiment, the inoculation amount of the bacterial solution is 10%;

[0092] S219: After the fermentation is completed, the fermentation liquid is placed in a centrifuge for centrifugation to obtain a fermentation liquid;

[0093] S220: pumping the fermentation liquid into the ion exchange column, the trehalose in the fermentation liquid is exchanged with the ions on the ion exchange resin in the ion exchange column, the ion exchange column is eluted with the eluent, the trehalose is eluted from the ion exchange resin, and the eluent, i.e., the trehalose solution, is collected.

[0094] The hydroxyl groups in the molecules of trehalose are all hydrophilic ethanes, which can effectively lock the moisture in the aqueous solution and play a role in water retention. At the same time, trehalose is a nutrient that is easily absorbed by the small intestine and turned into energy. It is non-toxic and can provide nutrients to the human body after residue.

[0095] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological antibacterial agent, characterized in that: Includes the following components: Gaseous available chlorine, Oxalis extract, sericin and trehalose; Gaseous available chlorine is obtained by injecting saturated salt water into an electrolytic cell for electrolysis to generate sodium hydroxide solution, chlorine gas and hydrogen gas. The sodium hydroxide solution, chlorine gas and hydrogen gas are separated and purified to obtain chlorine gas, which is gaseous available chlorine. The Oxalis extract is prepared by reacting carbon monoxide with separated and purified hydrogen to generate methanol, fresh Oxalis is picked, washed, dried, and crushed, the crushed Oxalis residue and the generated methanol are placed in a solvent container, the Oxalis residue is immersed in methanol, the solvent container is heated, the Oxalis residue and methanol are heated, the methanol evaporates to form steam, the steam rises to a condenser, cools and refluxes into the solvent container until the heating time is up, the heating is stopped, the extract in the solvent container is filtered, and a methanol extract of quercetin is obtained, and the quercetin is separated and purified by adsorption with DM-130 adsorption resin to obtain the quercetin, that is, the Oxalis extract; Sericin is prepared by cutting high-quality silkworm cocoons into small pieces with scissors, washing with deionized water to remove surface impurities, mixing with separated and purified sodium hydroxide solution, preheating with waste heat from a condenser tube and then reheating with a heater, stirring and mixing, waiting for the silkworm cocoons to dissolve in the sodium hydroxide solution until they are completely dissolved, filtering the mixture to remove insoluble impurities, centrifuging to separate the sericin solution, and freeze-drying to remove moisture from the sericin solution; Trehalose is prepared by inoculating lactobacillus onto a slant medium for activation culture, then inoculating it into a shake flask medium for shake flask culture to allow the strain to reach a logarithmic growth phase, then inoculating it into a fermenter for fermentation, and then centrifuging and purifying it.

2. The method for preparing a biological antibacterial agent according to claim 1, characterized in that: The following steps are involved: S1: Pass gaseous available chlorine into separated and purified sodium hydroxide solution or pure water to adjust the pH value until the pH value reaches 8.5-9.5; S2: Place Oxalis sorrel extract, sericin and trehalose in a volume ratio of 1:1 with gaseous available chlorine into the above solution, stir and mix until the mixture is uniform, and prepare a biological antibacterial agent.

3. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: In step S1, the specific steps of gaseous available chlorine include: S101: Design and build an electrolytic cell with adjustable current, voltage and temperature parameters. Install anodes and cathodes in the electrolytic cell, and connect the anodes and cathodes to an external power source through wires; S102: Inject saturated salt water into the electrolytic cell and turn on the power supply. The current flows between the anode and the cathode, triggering an electrolytic reaction. An oxidation reaction occurs at the anode and a reduction reaction occurs at the cathode, generating sodium hydroxide solution, chlorine gas and hydrogen gas. The chlorine gas is the gaseous available chlorine.

4. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: In step S1, the sodium hydroxide solution is the sodium hydroxide solution separated and purified in step S102.

5. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: In step S2, the specific steps of extracting Oxalis Sorrel include: S201: constructing a reaction system structure, including a solvent container A, a heater A, a condenser and a cooler, wherein the solvent container A is placed in the heater A, the condenser is placed above the solvent container A and fully covers the opening of the solvent container A, the cooler covers the condenser, and the condenser is located above the solvent container A; S202: picking fresh Oxalis, washing, drying, and placing in a crushing container for crushing; S203: reacting carbon monoxide with the hydrogen separated and purified in step S102 to generate methanol; S204: placing the crushed Oxalis residue and methanol in a solvent container A, so that the methanol submerges the Oxalis residue; S205: Heater A heats the Oxalis residue and methanol. During the heating process, methanol evaporates to form steam. The steam rises to the condenser. Under the action of the cooler providing a cold source, the steam is cooled into liquid in the condenser and flows back into the solvent container A. S206: When the set reaction time is reached, the heating is stopped, and the extract in the solvent container A is placed in a filter container to filter out the residue to obtain a methanol extract of quercetin; S207: quercetin, i.e., Oxalis sorrel extract, is separated and purified by adsorption using DM-130 adsorption resin.

6. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: In step S2, the specific steps of preparing sericin include: S208: constructing a reaction system structure, including a solvent container B and a heater B, wherein the condenser in step S201 is wound around the outer wall of the solvent container B, the cooler is placed at the end of the condenser, and the solvent container B is placed in the heater B; S209: Prepare the finest silkworm cocoons; S210: Cut the cocoons into small pieces using scissors; S211: Clean the small pieces of silk cocoons with deionized water to remove surface impurities; S212: placing the cleaned small pieces of silk cocoons in a solvent container B, adding the sodium hydroxide solution separated and purified in step S102, first preheating them with the residual heat of the steam in the wound condenser tube, and then heating them with the heater B, stirring and mixing during the heating process, and waiting for the silk cocoons to dissolve in the sodium hydroxide solution; S213: After being completely dissolved, the mixture is placed in a filtering container to filter out insoluble impurities; S214: placing the filtrate in a centrifugal processor for centrifugal treatment to separate the sericin solution; S215: placing the sericin solution in a freeze dryer for freeze drying to remove moisture in the sericin solution and obtain sericin protein.

7. The method for preparing a biological antibacterial agent according to claim 2, characterized in that: In step S2, the specific steps of adding trehalose include: S216: inoculating lactobacillus onto the slant culture medium for activation culture; S217: inoculating the activated strain from the slant culture medium into the shake flask culture medium, and culturing the strain in the shake flask until the strain reaches the logarithmic growth phase; S218: inoculating the bacterial liquid obtained by the shake flask culture into a fermentation tank for fermentation; S219: After the fermentation is completed, the fermentation liquid is placed in a centrifuge for centrifugation to obtain a fermentation liquid; S220: pumping the fermentation liquid into the ion exchange column, the trehalose in the fermentation liquid is exchanged with the ions on the ion exchange resin in the ion exchange column, the ion exchange column is eluted with the eluent, the trehalose is eluted from the ion exchange resin, and the eluent, i.e., the trehalose solution, is collected.

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