Medicinal ointment for preventing and treating cow mastitis and preparation method thereof

Abstract

The invention discloses a medicinal ointment for preventing and treating cow mastitis and a preparation method thereof, and belongs to the technical field of biology. According to the invention, dandelion, honeysuckle, oldenlandia diffusa, herba violae, centella asiatica, radix paeoniae alba and other Chinese herbal medicines with the effects of clearing away heat and toxic materials and activating blood and promoting lactation are selected as raw materials, active ingredients of the Chinese herbal medicines are extracted by using a biological enzymolysis technology, and polysaccharide and the like are supplemented to prepare the medicinal ointment, so that the prevention and treatment effect of the medicinal ointment on cow mastitis is remarkably improved; the advantages of small toxic and side effects, no drug residue and difficulty in generating drug resistance of Chinese herbal medicines are fully exerted; besides, active substances such as polysaccharide, saponin and flavone in Chinese herbal medicines such as dandelion and honeysuckle are fully extracted through an extraction process combining a biological compound enzymolysis method and ethanol extraction, and algal polysaccharides such as carrageenan polysaccharide, alginate oligosaccharide and green algae polysaccharide are added, so that the health-care food is prepared. The prevention and treatment effects of the medicinal ointment are further improved through the antiviral, antibacterial and anti-inflammatory effects of the algal polysaccharides.

Description

Technical Field

[0001] The invention relates to a medicinal ointment for preventing and treating cow mastitis and a preparation method thereof, in particular to a method for preparing the medicinal ointment for preventing cow mastitis with high efficiency by using a biological enzymolysis method, and belongs to the field of biotechnology. Background Art

[0002] In recent years, my country's dairy farming industry has developed rapidly, and the scale of farming has continued to expand. However, cow mastitis, especially latent mastitis, has a high incidence rate and is difficult to treat, which greatly affects the performance of dairy cow production and seriously restricts the development of the dairy industry. There are many pathogenic microorganisms that cause cow mastitis, among which Escherichia coli, Staphylococcus aureus and Streptococcus dysgalactiae are the most common pathogens. Escherichia coli is the most common Gram-negative bacteria, and the mastitis caused by it can cause the death of dairy cows in severe cases; Staphylococcus aureus is the most common Gram-positive pathogen associated with various types of mastitis, which often leads to the occurrence of chronic mastitis. Among them, methicillin-resistant Staphylococcus aureus will produce biofilms, leading to increased drug resistance, making the treatment of such infections more difficult; Streptococcus dysgalactiae is a Gram-positive pathogen that causes high somatic cell counts and low milk production in infected cows. It is a common zoonotic pathogen that poses a threat to human public health and safety.

[0003] At present, antibiotic treatment is the main treatment for bovine mastitis. Although antibiotics can effectively kill invading pathogens and bacteria, long-term use of antibiotics has the disadvantages of drug resistance affecting the efficacy and excessive antibiotic residues, which restricts the application of antibiotics in dairy farming. The effective ingredients of Chinese herbal medicine and other plant sources are pure natural substances from nature, with advantages such as no drug residue, high efficiency and low toxicity, and not easy to produce drug resistance. They have broad application prospects in the treatment of bovine mastitis and can make up for the defects of drug resistance and antibiotic residues that are easily caused by antibiotics. Although Chinese herbal medicine is more and more widely used in the treatment of bovine mastitis, there are still problems such as poor efficacy and huge differences, and large differences in the efficacy of mastitis caused by different pathogens. It is urgent to develop efficient, popular and green Chinese herbal preparations, such as medicinal ointments, to achieve green and efficient prevention and treatment of mastitis in dairy farming. Summary of the invention

[0004] In order to overcome the shortcomings of the prior art, the purpose of the present invention is to provide a medicinal ointment for preventing and treating cow mastitis and a preparation method thereof, wherein Chinese herbal medicines such as heat-clearing and detoxifying, promoting blood circulation and promoting lactation are selected as raw materials, the active ingredients of the Chinese herbal medicines are extracted by biological enzymatic hydrolysis technology, and the medicinal ointment is prepared with the aid of polysaccharides, etc., which significantly improves the preventive and therapeutic effect of the medicinal ointment on cow mastitis, and gives full play to the advantages of Chinese herbal medicines with small toxic and side effects, no drug residue, and not prone to drug resistance.

[0005] The above object of the present invention is achieved through the following technical solutions: The invention provides a medicinal ointment for preventing and treating cow mastitis. The raw materials include, by mass, 5-10 parts of compound Chinese herbal medicine extract paste, 0.05-0.1 parts of polysaccharide, 10-20 parts of auxiliary emulsifier, 10-20 parts of liquid paraffin, 30-50 parts of white vaseline, 0.05-0.2 parts of antioxidant and 0.1-0.2 parts of preservative.

[0006] Preferably, the present invention provides a medicinal ointment for preventing and treating bovine mastitis, wherein the raw materials include 8 parts of compound Chinese herbal medicine extract, 0.07 parts of polysaccharide, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant and 0.1 parts of preservative in parts by mass.

[0007] Preferably, the Chinese herbal medicine extract is prepared by the following process: (1) Compatibility of Chinese herbal medicines: accurately match Chinese herbal medicines according to the ratio of 10-20 parts of dandelion, 5-10 parts of honeysuckle, 6-12 parts of oldenlandia diffusa, 5-10 parts of plantain, 3-5 parts of centella asiatica, 3-5 parts of white peony root, and 1-2 parts of liquorice; (2) Grinding and mixing: Grind the Chinese herbal medicine raw materials into 40-60 meshes and mix them evenly to obtain the compound Chinese herbal medicine powder; (3) Ultrasonic-assisted enzymatic hydrolysis: add 5 times the amount of water to the compound Chinese herbal medicine powder, add a compound enzyme preparation at a ratio of 3% of the mass ratio of the Chinese herbal medicine, and reflux and degrade at 60°C for 60 min under the assistance of ultrasonic waves; (4) Filtration: After the enzymatic hydrolysis is completed, filter to obtain the Chinese herbal medicine enzymatic hydrolysate and Chinese herbal medicine residue; (5) Ethanol extraction: add 3 times the mass ratio of anhydrous ethanol to the Chinese herbal medicine residue and reflux extraction for 30 min; (6) Filtration: After ethanol extraction, filter to obtain the Chinese herbal medicine ethanol extract; (7) Combined: a Chinese herbal medicine extract obtained by combining the Chinese herbal medicine enzymatic hydrolysate and the ethanol extract; (8) Concentration: Concentrate the Chinese herbal medicine extract into a paste to obtain a Chinese herbal medicine extract paste.

[0008] Furthermore, the ratio of the Chinese herbal medicines is 15 parts of dandelion, 8 parts of honeysuckle, 9 parts of oldenlandia diffusa, 7.5 parts of plantain, 4.2 parts of centella asiatica, 3.6 parts of white peony root, and 1.8 parts of licorice.

[0009] Preferably, the complex enzyme preparation is a complex enzyme preparation of cellulase, non-starch polysaccharide enzyme, pectinase and protease, and the composition ratio thereof is 5:1:2:1.

[0010] Preferably, the polysaccharide is one or a mixture of carrageenan polysaccharide, brown algae oligosaccharide, and green algae polysaccharide.

[0011] Preferably, the polysaccharide is a mixture of carrageenan polysaccharide and chlorella polysaccharide, with a mass ratio of 3:2.

[0012] Preferably, the auxiliary emulsifier is one of monostearate or lecithin.

[0013] Preferably, the antioxidant is alkyl gallate or butylated hydroxytoluene; and the preservative is ethyl hydroxybenzoate.

[0014] The present invention also provides a method for preparing a medicinal ointment for preventing and treating cow mastitis, which specifically comprises the following steps: heating white vaseline to a molten state, adding liquid paraffin and an auxiliary emulsifier, and stirring evenly; then sequentially adding a Chinese herbal medicine extract, a polysaccharide, an antioxidant, and a preservative, homogenizing, and cooling to obtain the medicinal ointment for preventing and treating cow mastitis.

[0015] Preferably, the process of heating white vaseline to a molten state is: firstly heating the white vaseline to 80-90°C, and then cooling it to 50-55°C after it is completely melted.

[0016] Preferably, the homogenization speed is 4000-5000 rpm and the time is 5-10 min.

[0017] The present invention provides a medicinal ointment for preventing and treating cow mastitis and a preparation method thereof, which has the following significant advantages over the prior art: (1) The present invention uses Chinese herbal medicines such as dandelion, honeysuckle, oldenlandia diffusa, plantain, gout, and white peony root that have the functions of clearing away heat and detoxifying, promoting blood circulation and promoting lactation as raw materials, uses biological enzymatic hydrolysis technology to extract the active ingredients of Chinese herbal medicines, and prepares them into medicinal ointment with the aid of polysaccharides, etc., which significantly improves the preventive and therapeutic effect of the medicinal ointment on bovine mastitis, and gives full play to the advantages of Chinese herbal medicines with small toxic and side effects, no drug residue, and not prone to drug resistance; (2) Different Chinese herbal medicines have different properties and flavors, and their effects vary greatly. There are complex interactions between different Chinese herbal medicines when they are combined, and the ratios between Chinese herbal medicines are relatively close. Different ratios will also cause substantial changes in the relationship between monarch, minister, assistant, and envoy, thereby causing substantial changes in the relationship between the compound Chinese herbal medicines. The present invention achieves significant technical effects in the medicinal ointment product for preventing and treating bovine mastitis by scientifically combining Chinese herbal medicines such as dandelion, honeysuckle, oldenlandia diffusa, plantain, gout, and white peony root, and by using the complex relationships between the Chinese herbal medicines. The test results show that the synergistic effect between Chinese herbal medicines is the technical effect of "1+1>2" achieved by the product of the present invention; (3) The present invention uses an extraction process that combines biological composite enzymatic hydrolysis with ethanol extraction to fully extract active substances such as polysaccharides, saponins, and flavonoids from Chinese herbal medicines such as dandelions and honeysuckle. At the same time, the extraction process promotes the reaction and transformation between Chinese herbal medicines, further improving the prevention and treatment effect of bovine mastitis; (4) The present invention adds seaweed polysaccharides such as carrageenan polysaccharides, brown algae oligosaccharides, and green algae polysaccharides to the medicinal ointment, and further improves the preventive and therapeutic effects of the medicinal ointment through the antiviral, antibacterial, and anti-inflammatory effects of the seaweed polysaccharides; at the same time, through the biological activity of seaweed polysaccharides, the permeability of active ingredients of Chinese herbal medicines on the skin surface is promoted, significantly improving the preventive and therapeutic effects of the product; (5) The present invention prepares the therapeutic drug into an ointment, which is not only convenient for administration, but also gives full play to the advantages of Chinese medicine, such as low toxicity and side effects, low resistance to drug resistance, and no drug residue. At the same time, the preparation method provided by the present invention is simple, which is conducive to large-scale promotion and application. DETAILED DESCRIPTION

[0018] The Chinese herbal medicine extracts described in the following examples are prepared according to the following steps: (1) Compatibility of Chinese herbal medicines: accurately prepare the Chinese herbal medicines according to the compatibility ratio of the Chinese herbal medicines in the examples; (2) Grinding and mixing: Grind the Chinese herbal medicine raw materials into 40-60 meshes and mix them evenly to obtain the compound Chinese herbal medicine powder; (3) Ultrasonic-assisted enzymatic hydrolysis: add 5 times of water to the compound Chinese herbal medicine powder, add a composite enzyme preparation at a ratio of 3% of the mass ratio of the Chinese herbal medicine, and reflux and degrade at 60°C for 60 minutes under the assistance of ultrasonic waves; the composite enzyme preparation is a composite enzyme preparation of cellulase, non-starch polysaccharide enzyme, pectinase, and protease, and its composition ratio is 5:1:2:1; (4) Filtration: After the enzymatic hydrolysis is completed, filter to obtain the Chinese herbal medicine enzymatic hydrolysate and Chinese herbal medicine residue; (5) Ethanol extraction: add 3 times the mass ratio of anhydrous ethanol to the Chinese herbal medicine residue and reflux extraction for 30 min; (6) Filtration: After ethanol extraction, filter to obtain the Chinese herbal medicine ethanol extract; (7) Combined: a Chinese herbal medicine extract obtained by combining the Chinese herbal medicine enzymatic hydrolysate and the ethanol extract; (8) Concentration: Concentrate the Chinese herbal medicine extract into a paste to obtain a Chinese herbal medicine extract paste.

[0019] The medicinal ointment described in the following examples is prepared according to the following steps: white vaseline is heated to 80-90° C., and after being completely melted, the temperature is lowered to 50-55° C.; liquid paraffin and an auxiliary emulsifier are then added and stirred evenly; then Chinese herbal medicine extract, polysaccharide, antioxidant, and preservative are added in sequence, homogenized, and cooled to obtain a medicinal ointment for preventing and treating bovine mastitis. Example

[0020] A medicinal ointment for preventing and treating mastitis in dairy cows, comprising the following raw materials by mass: 8 parts of compound Chinese herbal medicine extract, 0.07 parts of polysaccharide, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant, and 0.1 parts of preservative; The proportion of the Chinese herbal medicines is 15 parts of dandelion, 8 parts of honeysuckle, 9 parts of oldenlandia diffusa, 7.5 parts of plantain, 4.2 parts of centella asiatica, 3.6 parts of white peony root, and 1.8 parts of liquorice; The polysaccharide is a mixture of carrageenan polysaccharide and green algae polysaccharide, and the mass ratio thereof is 3:2; The auxiliary emulsifier is monostearate of fatty acid; The antioxidant is alkyl gallate or butyl hydroxytoluene, and the preservative is ethyl hydroxybenzoate. Example

[0021] A medicinal ointment for preventing and treating mastitis in dairy cows, comprising the following raw materials by mass: 8 parts of compound Chinese herbal medicine extract, 0.07 parts of polysaccharide, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant, and 0.1 parts of preservative; The proportion of the Chinese herbal medicines is 20 parts of dandelion, 10 parts of honeysuckle, 12 parts of oldenlandia diffusa, 10 parts of plantain, 5 parts of centella asiatica, 5 parts of white peony root, and 2 parts of liquorice; The polysaccharide is a mixture of carrageenan polysaccharide and green algae polysaccharide, and the mass ratio thereof is 3:2; The auxiliary emulsifier is monostearate of fatty acid; The antioxidant is alkyl gallate or butyl hydroxytoluene, and the preservative is ethyl hydroxybenzoate. Example

[0022] A medicinal ointment for preventing and treating mastitis in dairy cows, comprising the following raw materials by mass: 8 parts of compound Chinese herbal medicine extract, 0.07 parts of polysaccharide, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant, and 0.1 parts of preservative; The proportion of the Chinese herbal medicines is 15 parts of dandelion, 7.5 parts of honeysuckle, 9 parts of oldenlandia diffusa, 7.5 parts of plantain, 4 parts of centella asiatica, 4 parts of white peony root, and 1.5 parts of liquorice; The polysaccharide is a mixture of carrageenan polysaccharide and green algae polysaccharide, and the mass ratio thereof is 3:2; The auxiliary emulsifier is monostearate of fatty acid; The antioxidant is alkyl gallate or butyl hydroxytoluene, and the preservative is ethyl hydroxybenzoate. Example

[0023] A medicinal ointment for preventing and treating mastitis in dairy cows, comprising the following raw materials by mass: 8 parts of compound Chinese herbal medicine extract, 0.07 parts of polysaccharide, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant, and 0.1 parts of preservative; The proportion of the Chinese herbal medicines is 15 parts of dandelion, 8 parts of honeysuckle, 9 parts of oldenlandia diffusa, 7.5 parts of plantain, 4.2 parts of centella asiatica, 3.6 parts of white peony root, and 1.8 parts of liquorice; The polysaccharide is carrageenan polysaccharide; The auxiliary emulsifier is monostearate of fatty acid; The antioxidant is alkyl gallate or butyl hydroxytoluene, and the preservative is ethyl hydroxybenzoate. Example

[0024] A medicinal ointment for preventing and treating mastitis in dairy cows, comprising the following raw materials by mass: 8 parts of compound Chinese herbal medicine extract, 0.07 parts of polysaccharide, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant, and 0.1 parts of preservative; The proportion of the Chinese herbal medicines is 15 parts of dandelion, 8 parts of honeysuckle, 9 parts of oldenlandia diffusa, 7.5 parts of plantain, 4.2 parts of centella asiatica, 3.6 parts of white peony root, and 1.8 parts of liquorice; The polysaccharide is green algae polysaccharide; The auxiliary emulsifier is monostearate of fatty acid; The antioxidant is alkyl gallate or butyl hydroxytoluene, and the preservative is ethyl hydroxybenzoate.

[0025] A medicinal ointment for preventing and treating mastitis in dairy cows, comprising the following raw materials by mass: 8 parts of compound Chinese herbal medicine extract, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant, and 0.1 parts of preservative; The proportion of the Chinese herbal medicines is 15 parts of dandelion, 8 parts of honeysuckle, 9 parts of oldenlandia diffusa, 7.5 parts of plantain, 4.2 parts of centella asiatica, 3.6 parts of white peony root, and 1.8 parts of liquorice; The auxiliary emulsifier is monostearate of fatty acid; The antioxidant is alkyl gallate or butyl hydroxytoluene, and the preservative is ethyl hydroxybenzoate.

[0026] (1) Test sample treatment: The medicinal ointments prepared in Examples 1-5 and Comparative Example 1 were diluted 10 times for later use; (2) Take Staphylococcus aureus and Escherichia coli grown on ordinary nutrient agar plates, dip a small amount of colonies with an inoculation loop, inoculate into 5 mL of MH broth, and culture at 37°C and 200 r / min for 6 h; take the bacterial solution in the logarithmic growth phase and dilute it with sterile saline to a bacterial count of 1 to 2 × 108 CFU / mL; then dilute 1000 times with sterile saline for later use; (3) Use a punch to make a filter paper disc with a diameter of 6.5 mm, dry heat sterilize, first evenly inoculate the bacterial solution obtained in step (2) into a nutrient agar culture dish, then attach the sterilized filter paper disc to the agar surface, use a micropipette to transfer 20 μL of the test sample in step (1) and drop it on the center of the filter paper disc, incubate at 37°C for 24 h, and measure the diameter of the inhibition zone of the ointment of different embodiments.

[0027] The test results are shown in the following table: Table 1 Antibacterial results of medicinal ointments in different embodiments Group measurement indicators Staphylococcus aureus inhibition zone diameter (mm) Diameter of Escherichia coli inhibition zone (mm) Example 1 23.5 21.6 Example 2 20.6 17.3 Example 3 21.1 15.4 Example 4 19.2 17.5 Example 5 20.1 18.1 Comparative Example 1 17.4 15.9 The above experimental results show that the medicinal ointment for preventing and treating bovine mastitis of the present invention has significant antibacterial effect, among which Example 1 has the best antibacterial effect and is the best embodiment of the present invention; the experimental results of Examples 2-3 show that the compatibility ratio of Chinese herbal medicines in the present invention has a significant effect on the antibacterial effect of the ointment, and the difference is significant, which indicates that the synergistic effect between Chinese herbal medicines is the fundamental reason for the efficacy of the medicinal ointment of the present invention; the experimental results of Examples 4-5 show that adding polysaccharides in the present invention has a significant effect on improving the antibacterial effect; compared with the results of the comparative example, it can be found that the synergistic effect between carrageenan polysaccharides and green algae polysaccharides in the technical scheme of the present invention has achieved the technical effect of "1+1>2".

[0028] Experimental scheme: In a farm in Henan Province, 80 cows with mastitis were detected by mastitis test method (SMT), of which 20 cows were externally coated with the medicinal ointment for preventing and treating mastitis in cows prepared in Example 1, 20 cows were externally coated with the medicinal ointment for preventing and treating mastitis in cows prepared in Example 2, and 20 cows were externally coated with the medicinal ointment for preventing and treating mastitis in cows prepared in Example 3, twice a day for 5 days, with each dosage of 1 g; the remaining 20 cows were drained of all milk from their udders, and 100,000 IU of penicillin was infused into their udders as a control group; Cure criteria: After stopping medication for 1 day, the patient is considered cured if the sodium hydroxide curd test is negative.

[0029] The test results are shown in Table 2: Table 2 Results of different examples of medicinal ointments for preventing and treating mastitis Group measurement indicators Cows with mastitis (heads) Number of cured cows (head) Cure rate (%) Example 1 20 19 95 Example 2 20 16 80 Example 3 20 15 75 Control group 20 18 90 The above experimental results show that the medicinal ointment described in Example 1 of the present invention has a significant technical effect in preventing and treating mastitis, and the cure rate is higher than that of the antibiotic control group; the experimental results of Examples 2-3 show that the prepared medicinal ointment has a certain curative effect, but due to the difference in the compatibility ratio of Chinese herbal medicines, the curative effect is lower than that of the antibiotic control group.

[0030] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the invention has been described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to replace some of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions claimed to be protected by the present invention.

Claims

1. A medicinal ointment for preventing and treating mastitis in dairy cows, characterized in that The raw materials include, by mass, 5 to 10 parts of compound Chinese herbal medicine extract, 0.05 to 0.1 parts of polysaccharides, 10 to 20 parts of auxiliary emulsifiers, 10 to 20 parts of liquid paraffin, 30 to 50 parts of white vaseline, 0.05 to 0.2 parts of antioxidants, and 0.1 to 0.2 parts of preservatives.

2. A medicinal ointment for preventing and treating cow mastitis according to claim 1, characterized in that The raw materials include 8 parts of compound Chinese herbal medicine extract, 0.07 parts of polysaccharide, 20 parts of auxiliary emulsifier, 15 parts of liquid paraffin, 40 parts of white vaseline, 0.1 parts of antioxidant and 0.1 parts of preservative in parts by mass.

3. A medicinal ointment for preventing and treating cow mastitis according to claim 1, characterized in that The Chinese herbal medicine extract is prepared by the following process: (1) Compatibility of Chinese herbal medicines: accurately match Chinese herbal medicines according to the ratio of 10-20 parts of dandelion, 5-10 parts of honeysuckle, 6-12 parts of oldenlandia diffusa, 5-10 parts of plantain, 3-5 parts of centella asiatica, 3-5 parts of white peony root, and 1-2 parts of liquorice; (2) Grinding and mixing: Grind the Chinese herbal medicine raw materials into 40-60 meshes and mix them evenly to obtain the compound Chinese herbal medicine powder; (3) Ultrasonic-assisted enzymatic hydrolysis: add 5 times the amount of water to the compound Chinese herbal medicine powder, add a compound enzyme preparation at a ratio of 3% of the mass ratio of the Chinese herbal medicine, and reflux and degrade at 60°C for 60 min under the assistance of ultrasonic waves; (4) Filtration: After the enzymatic hydrolysis is completed, filter to obtain the Chinese herbal medicine enzymatic hydrolysate and Chinese herbal medicine residue; (5) Ethanol extraction: add 3 times the mass ratio of anhydrous ethanol to the Chinese herbal medicine residue and reflux extraction for 30 min; (6) Filtration: After ethanol extraction, filter to obtain the Chinese herbal medicine ethanol extract; (7) Combined: a Chinese herbal medicine extract obtained by combining the Chinese herbal medicine enzymatic hydrolysate and the ethanol extract; (8) Concentration: Concentrate the Chinese herbal medicine extract into a paste to obtain a Chinese herbal medicine extract paste.

4. A medicinal ointment for preventing and treating cow mastitis according to claim 4, characterized in that The proportions of the Chinese herbal medicines are 15 parts of dandelion, 8 parts of honeysuckle, 9 parts of oldenlandia diffusa, 7.5 parts of plantain, 4.2 parts of centella asiatica, 3.6 parts of white peony root and 1.8 parts of liquorice.

5. A medicinal ointment for preventing and treating cow mastitis according to claim 3, characterized in that The complex enzyme preparation is a complex enzyme preparation of cellulase, non-starch polysaccharide enzyme, pectinase and protease, and the composition ratio thereof is 5:1:2:

1.

6. The medicinal ointment for preventing and treating cow mastitis according to claim 1, characterized in that The polysaccharide is one or a mixture of carrageenan polysaccharide, brown algae oligosaccharide and green algae polysaccharide.

7. A medicinal ointment for preventing and treating cow mastitis according to claim 6, characterized in that The polysaccharide is a mixture of carrageenan polysaccharide and green algae polysaccharide, and the mass ratio thereof is 3:

2.

8. The medicinal ointment for preventing and treating cow mastitis according to claim 1, characterized in that The auxiliary emulsifier is one of monostearate or lecithin.

9. The medicinal ointment for preventing and treating cow mastitis according to claim 1, characterized in that The antioxidant is alkyl gallate or butyl hydroxytoluene; the preservative is ethyl hydroxybenzoate.

10. The medicinal ointment for preventing and treating cow mastitis according to claim 1, characterized in that The preparation method of the medicinal ointment specifically comprises the following steps: heating white vaseline to a molten state, adding liquid paraffin and an auxiliary emulsifier, and stirring evenly; then sequentially adding Chinese herbal medicine extract, polysaccharide, antioxidant, and preservative, homogenizing, and cooling to obtain the medicinal ointment for preventing and treating cow mastitis.