A motion-driven self-powered hydrogel nanofiber patch and its preparation method and application

Abstract

The present invention discloses a motion-driven self-powered hydrogel nanofiber patch, a preparation method and an application thereof. The invention comprehensively utilizes the anti-inflammatory / anti-oxidation properties of GelMA hydrogel nanofibers, TA and the piezoelectric effect of tetragonal barium titanate to develop a motion-driven self-powered hydrogel nanofiber patch, which can accelerate wound healing by regulating immune response, inflammation, promoting cell proliferation and adhesion, and forming a state closest to the original skin.

Description

Technical Field

[0001] The present invention relates to a motion-driven self-powered hydrogel nanofiber patch and a preparation method and application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance some understanding of the overall background of the invention and should not be necessarily regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] Skin wounds are common in accidental injuries. Slow healing and scarring can severely impact daily life. Wound healing requires careful consideration. On the one hand, it is crucial to ensure accelerated re-epithelialization, collagen deposition, and angiogenesis; on the other hand, it is crucial to prevent excessive wound inflammation, which can lead to scarring. Under external force, continuous mechanical deformation generates electrical stimulation directly on the wound. However, the development of self-powered skin patches with robust biological functions to address wound healing and leverage both the biomaterial and electrical stimulation is challenging. To this end, researchers have developed biomaterials with piezoelectric properties (DOI:10.1002 / smll.202301981). These patches are then modified with active molecules to impart additional functionalities, such as anti-inflammatory and antioxidant properties (DOI:10.1021 / acsami.6b10491). However, conventional self-powered skin patches generally focus on piezoelectric response. While effective, they are limited by the inherent material properties. For example, the material itself is non-degradable and difficult to perfectly conform to the wound surface. Consequently, complex, irregular wounds and inflammatory environments have not yet received much research attention. For patients with chronic wounds, slow wound healing is also a clinical problem that needs to be urgently addressed. Summary of the Invention

[0004] In response to the shortcomings of the existing technology and in view of the mechanical deformation caused by daily exercise, the present invention has developed a motion-driven, self-powered hydrogel nanofiber patch based on the piezoelectric response characteristics of mechanical deformation and the piezoelectric effect of barium titanate. On this basis, the patch is given anti-inflammatory / antioxidant capabilities to synergistically accelerate the wound healing process and solve the various problems faced by internal wounds in the body.

[0005] The technical solution adopted in the present invention is as follows:

[0006] In a first aspect of the present invention, a method for preparing a motion-driven self-powered hydrogel nanofiber patch is provided, the method comprising the following steps:

[0007] (1) preparing a spinning solution containing GelMA (gelatin methacrylate anhydride) and tetragonal barium titanate nanoparticles as solutes, and obtaining piezoelectric nanofibers after electrospinning;

[0008] (2) drying the piezoelectric nanofibers prepared in step (1), and then immersing the dried piezoelectric nanofibers in a photocrosslinking solution, crosslinking the piezoelectric nanofibers under ultraviolet irradiation, to obtain piezoelectric hydrogel nanofibers;

[0009] (3) The piezoelectric hydrogel nanofiber is immersed in a TA (tannic acid) mixed solution and taken out after immersion to obtain a functionally modified piezoelectric hydrogel fiber patch, which is the motion-driven self-powered hydrogel nanofiber patch of the present invention.

[0010] In one or some embodiments of the present invention, in step (1), the degree of substitution of GelMA is 0-100%, excluding 0; preferably, the degree of substitution of GelMA is 10-80%; more preferably, the degree of substitution of GelMA is 60%.

[0011] In one or some embodiments of the present invention, in step (1), the amount of tetragonal barium titanate nanoparticles added is 0-50% (w / v), excluding 0, which means that 0-50g of tetragonal barium titanate nanoparticles are added to 100mL of solution (added with GelMA solution); preferably, the amount of tetragonal barium titanate nanoparticles added is 0.1-20% (w / v); more preferably, the amount of tetragonal barium titanate nanoparticles added is 0.25% (w / v). The addition of tetragonal barium titanate will affect the piezoelectric properties of the material, and the increase of barium titanate can increase the piezoelectric output. However, when the concentration of barium titanate is too high, its biocompatibility will be reduced. After experimental verification, 0-50% (w / v) is selected.

[0012] In one or some embodiments of the present invention, in step (1), the solvent of the spinning solution is HFIP (hexafluoroisopropanol), and the concentration of the spinning solution is 1-20% (w / v), which means that 1-20 g of GelMA is added to 100 mL of HFIP.

[0013] In one or some embodiments of the present invention, in step (1), the electrospinning parameters are: voltage 9-10 kV, injection rate 20 μL / min, receiving distance 20 cm, drum speed 200 rpm, and environmental conditions maintained at 20-25° C. and 25-30% humidity.

[0014] In one or some embodiments of the present invention, in step (2), in order to obtain piezoelectric hydrogel nanofibers with good cross-linking effect, the photocrosslinking solution is prepared by dissolving a photoinitiator in anhydrous ethanol, and the concentration of the photocrosslinking solution is 1-20% (w / v), which means that 1-20g of photoinitiator is dissolved in 100mL of anhydrous ethanol; preferably, the solubility of the photocrosslinking solution is 1-10% (w / v); more preferably, the solution concentration is 5% (w / v).

[0015] Preferably, the photoinitiator is Irgacure 2959.

[0016] In one or some embodiments of the present invention, in step (2), during the cross-linking treatment, 365 nm ultraviolet irradiation is performed for 1 to 300 min; preferably, the ultraviolet irradiation is performed for 1 to 100 min; more preferably, the ultraviolet irradiation is performed for 10 min.

[0017] In one or some embodiments of the present invention, in step (3), the TA mixed solution is prepared by dissolving tannic acid in Tris-HCl buffer, with a concentration of 1 to 20 mg / mL; preferably, the concentration of the TA (tannic acid) mixed solution is 8 mg / mL.

[0018] In one or some embodiments of the present invention, in step (3), slight shaking is performed during soaking, with an shaking frequency of 10 to 1000 rpm.

[0019] In a second aspect of the present invention, a motion-driven self-powered hydrogel nanofiber patch prepared by the above method is provided.

[0020] Preferably, it is used for skin repair.

[0021] In a third aspect of the present invention, there is provided a use of the hydrogel nanofiber patch in the preparation of medical supplies.

[0022] Preferably, the hydrogel nanofiber patch is used in the preparation of skin repair products. Compared with the related art known to the inventors, one of the technical solutions of the present invention has the following beneficial effects:

[0023] The present invention comprehensively utilizes the piezoelectric effect of GelMA hydrogel nanofibers and tetragonal barium titanate to develop a motion-driven self-powered hydrogel nanofiber patch.

[0024] The present invention comprehensively utilizes GelMA hydrogel nanofibers, the anti-inflammatory / antioxidant properties of TA, and the piezoelectric effect of tetragonal barium titanate to develop a motion-driven, self-powered hydrogel nanofiber patch, which can accelerate wound healing by regulating immune response, inflammation, and promoting cell proliferation and adhesion, forming a state closest to the original skin.

[0025] This invention not only generates electrical stimulation driven by daily exercise to simulate endogenous bioelectric fields and promote wound healing, but also mimics the extracellular matrix to provide a suitable cell survival environment. The antioxidant and anti-inflammatory properties of TA can achieve immune regulation and inflammation control to accelerate wound healing. The electrospinning technology is low-cost and can be adapted to wounds of various sizes. In addition to skin injuries, it has greater application advantages in scenarios such as vascular and cardiac patches to promote tissue repair within the body.

[0026] The motion-driven self-powered hydrogel nanofiber patch of the present invention has the following functions: 1) The unique hydrogel nanofiber structure imitates the natural extracellular matrix environment to create a microenvironment most suitable for cell growth. The inflammatory response regulated by TA prevents the occurrence of excessive inflammation, which is crucial for patients with chronic diseases. It can prevent the wound from continuing to open and cause infection and scar formation, and promote wound repair; 2) The motion-driven self-powered hydrogel nanofiber converts mechanical energy into electrical stimulation to accelerate wound closure. Electrical stimulation that promotes wound healing can be generated remotely at any time, simulating endogenous bioelectric fields to solve various complex wound problems, while also dealing with wounds of different sizes. Figure 1 . BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings, which constitute a part of the specification of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0028] Figure 1 Schematic diagram of the hydrogel nanofiber patch of the present invention promoting skin healing.

[0029] Figure 2 Schematic diagram of the preparation principle of the hydrogel nanofiber patch of the present invention.

[0030] Figure 3 Morphological observation. GelMA: GelMA hydrogel nanofibers; Piezo: T-BTO-doped GelMA hydrogel nanofibers; Piezo@TA / Nonpiezo@TA: piezoelectric / non-piezoelectric hydrogel nanofibers coated with TA.

[0031] Figure 4 Analysis of material properties and piezoelectric effect: (ab) Optical images and quantitative curves of water contact angle variation over time; (c) Shape memory effect; (d) Height, piezoelectric amplitude, and phase images of barium titanate nanoparticles; (eg) Piezoelectric properties of tetragonal barium titanate nanoparticles; (hi) Piezoelectric response of Piezo and Piezo@TA to different pressures; (j) Comparison of piezoelectric responses of different materials at 25N.

[0032] Figure 5 Material biocompatibility evaluation: (a) cell viability, cell adhesion (30 min) and cell spreading (24 h); (b) changes in intracellular ROS expression and mitochondrial membrane potential.

[0033] Figure 6 Piezoelectric hydrogel nanofiber patch promotes skin healing: (a) Wound healing after different treatments; (b) Changes in wound healing marks and healing rate curve.

[0034] Figure 7 TA-modified piezoelectric hydrogel nanofiber patch promoted wound repair on day 14: (a) histological staining, immunofluorescence and immunohistochemistry; (bg) quantitative analysis.

[0035] Figure 8 TA-modified piezoelectric hydrogel nanofiber patch promoted wound repair on the 14th day: (a) Two-photon microscopy image of regenerated tissue; (b) quantitative analysis. DETAILED DESCRIPTION

[0036] It should be noted that the following detailed descriptions are exemplary and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs.

[0037] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations and / or combinations thereof.

[0038] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with reference to specific embodiments.

[0039] Example 1

[0040] Schematic diagram of preparation principle Figure 2 .

[0041] ① GelMA nanofiber bundles were prepared by stable jet electrospinning technology: GelMA (substitution degree of 60%) was weighed and dissolved in HFIP solution to prepare a spinning solution with a concentration of 5% (w / v). In order to give GelMA hydrogel nanofibers piezoelectric properties, 0.25% (w / v) tetragonal barium titanate nanoparticles (T-BTO) were added to the spinning solution, and then ultrasonicated for 15 minutes to ensure uniform dispersion. The solution was stirred on a magnetic stirrer for more than 12 hours until a uniform solution was formed, and ultrasonicated again for 15 minutes before electrospinning. Piezoelectric nanofibers were then prepared based on the electrospinning method. The spinning parameters were: voltage 9-10kV, injection rate 20μL / min, receiving distance 20cm, drum speed 200rpm, and environmental conditions were maintained at 20-25°C and 25-30% humidity. To induce the formation of hydrogel nanofibers, dried T-BTO-added GelMA nanofibers were soaked in a photocrosslinking solution (Irgacure 2959 dissolved in anhydrous ethanol at a concentration of 5% (w / v)) and irradiated with 365nm ultraviolet light for 10 minutes to induce crosslinking. Subsequently, the fibers were washed with anhydrous ethanol to remove excess photoinitiator, resulting in piezoelectric hydrogel nanofibers.

[0042] ②TA functional modified hydrogel nanofibers: tannic acid (TA, 8 mg / mL) was dissolved in Tris-HCl (10 mM, pH 8.5) buffer to prepare a TA mixed solution; the piezoelectric hydrogel nanofibers were then immersed in the solution and gently oscillated at room temperature for 12 h at an oscillation frequency of 100 rpm; the hydrogel nanofibers were taken out and washed with ultrapure water several times to obtain a TA functional modified piezoelectric hydrogel fiber patch.

[0043] like Figure 3 As shown, the GelMA hydrogel nanofiber membrane (named GelMA) and the hydrogel nanofiber membrane with piezoelectric properties (named Piezo) prepared by stable jet electrospinning technology both exhibit smooth surfaces. The piezoelectric / non-piezoelectric hydrogel nanofibers modified with TA (named Piezo@TA / Nonpiezo@TA) have thicker fiber diameters and an uneven fiber surface. These results demonstrate that the present invention successfully prepared GelMA hydrogel fiber membranes modified with piezoelectric / non-piezoelectric and TA functions.

[0044] like Figure 4 As shown in (a), the hydrophilicity of the hydrogel nanofibers modified with TA is significantly improved, which helps cells transport nutrients and metabolites. Quantitative analysis of the optical images shows Figure 4 (b) more intuitively reflects the change in the hydrophilicity of the hydrogel nanofibers. The mechanical properties of the fiber membrane changed significantly, indicating that tannic acid and barium titanate nanoparticles can change the mechanical properties of the piezoelectric hydrogel fiber membrane ( Figure 4 (c)); Subsequently, the present invention analyzed the piezoelectric properties of barium titanate nanoparticles and found that tetragonal barium titanate nanoparticles are beneficial to improving the piezoelectric properties of hydrogel nanofibers. Although cubic barium titanate nanoparticles have certain piezoelectric properties, they are still far lower than tetragonal barium titanate nanoparticles ( Figure 4 (dg)), which proves that tetragonal barium titanate has the potential to give hydrogel nanofibers piezoelectric properties; when the piezoelectric hydrogel nanofibers are placed under different pressures, it is found that the piezoelectric output generated by the pressure increases significantly ( Figure 4 (h)), the piezoelectric effect weakened to a certain extent after TA modification ( Figure 4 (i)). This may be attributed to the fact that TA makes the fiber network denser, resulting in smaller mechanical deformation under the same force, and TA has a certain binding and neutralizing effect on the generated electrons. Similarly, under the same force of 25N, the hydrogel nanofiber membrane without barium titanate has no piezoelectric effect, while the one doped with Nonpiezo@TA also has some changes ( Figure 4 (j)). These results indicate that tetragonal barium titanate can endow hydrogel nanofibers with good piezoelectric properties, and the hydrogel nanofiber membrane modified with TA has better hydrophilicity and is more suitable for cell proliferation and growth.

[0045] like Figure 5 As shown in (a), live-dead staining found that all material groups showed good cell compatibility, among which the Piezo@TA modified group had the largest proportion of live cells. At the same time, GelMA hydrogel nanofibers can promote fibroblast adhesion, and the promotion effect is further enhanced after TA and piezoelectric functional modification; similarly, when the cells are cultured for 24 hours, it can be observed that Piezo@TA modification significantly enhances the stress fiber remodeling of the cells and is also conducive to the formation of the cytoskeleton. From a functional point of view, TA modification can give the material antioxidant / anti-inflammatory ability, clear intracellular ROS to prevent inflammation and maintain the normal functional expression of mitochondria ( Figure 5 (b)).

[0046] like Figure 6 As shown in (a), the Piezo@TA patch was attached to the wound. The mechanical deformation caused by the mouse's daily movement caused the patch to produce electrical stimulation seams, thereby promoting wound healing. The wound was observed for 14 days. It can be seen that in the untreated Blank group, there was still a clear unclosed area at the wound ( Figure 6 (a)); The wound healing rate of Piezo@TA was significantly improved, and at 14. Interestingly, the healing rate of the Nonpiezo@TA group was also significantly higher than that of the Blank group ( Figure 6 (b) This indicates that the promotion of wound healing by Piezo@TA is the combined effect of electrical stimulation and TA-modified hydrogel nanofibers.

[0047] like Figure 7 (a) Analysis of wound changes over 14 days. Compared with the Blank group, hydrogel nanofiber treatment significantly promoted tissue remodeling in the wound area. It was observed that Piezo@TA significantly promoted skin regeneration, and wound healing was basically achieved under electrical stimulation, with obvious epithelialization ( Figure 7 (b) Masson staining results also confirmed that hydrogel nanofibers can promote collagen formation, and Nonpiezo@TA can also promote collagen remodeling and maturation. This phenomenon is further enhanced under electrical stimulation (Piezo@TA, Figure 7 (c)). PCNA / Integrinβ1 and CD31 immunofluorescence staining results further confirmed that Piezo@TA patches can significantly promote the proliferation of new cells and guide cell migration for epidermal remodeling under electrical stimulation ( Figure 7 (de)), considering that the density of CD31-positive capillaries decreased significantly during the remodeling phase Figure 7 (f), indicating that electrical stimulation of hydrogel nanofibers can accelerate skin healing and promote the wound to enter the remodeling stage. In addition, the TGF-β / IL-6 ratio of the Piezo@TA group was the highest ( Figure 7 (g)), indicating that a sustained anti-inflammatory microenvironment is beneficial for wound healing.

[0048] like Figure 8 (a) Analysis of skin changes after repair shows that compared with the Blank group, the regenerated epidermal tissue induced by the hydrogel nanofiber patch is very similar to the skin of normal rats. Under the dual effects of electrical stimulation and materials, Piezo@TA forms neatly arranged collagen fibers and elastic fibers, which indicates that electrical stimulation has a guiding effect on cell behavior and extracellular matrix remodeling. Specifically, since excessive thickness of the stratum corneum (SC) will delay wound healing, compared with normal skin tissue, the Blank group showed increased SC thickness and decreased granular layer (DSG) density ( Figure 8 (bc)). Therefore, after treatment with piezoelectric@TA and non-piezoelectric@TA, the SC and DSG were very similar to those of normal skin. The second harmonic generation (SHG)-autofluorescence (AF) dermal aging index (SAAID) reflects the changes in the average strength of collagen fibers and elastic fibers. Compared with the non-piezoelectric@TA group, the SAAID and dermal-epidermal junction index (DEJI) values detected in the piezoelectric@TA group were closer to those of the original skin ( Figure 8 (e)). This shows that the regenerated tissue is closest to the natural tissue under the dual effects of electrical stimulation and materials.

[0049] In summary, the present invention successfully prepared a motion-driven self-powered hydrogel nanofiber patch, and proved that it can not only use its own properties to accelerate wound closure, but also generate electrical stimulation under motion drive, further accelerating wound repair.

[0050] Example 2

[0051] ① GelMA nanofiber bundles were prepared by stable jet electrospinning technology: GelMA (substitution degree 65%) was weighed and dissolved in HFIP solution to prepare a spinning solution with a concentration of 10% (w / v). In order to give GelMA hydrogel nanofibers piezoelectric properties, 10% (w / v) tetragonal barium titanate nanoparticles (T-BTO) were added to the spinning solution, and then ultrasonicated for 20 minutes to ensure uniform dispersion. The solution was stirred on a magnetic stirrer for more than 14 hours until a uniform solution was formed, and ultrasonicated again for 20 minutes before electrospinning. Piezoelectric nanofibers were then prepared based on the electrospinning method. The spinning parameters were: voltage 9-10kV, injection rate 20μL / min, receiving distance 20cm, drum speed 200rpm, and environmental conditions were maintained at 20-25°C and 25-30% humidity. To induce the formation of hydrogel nanofibers, dried T-BTO-added GelMA nanofibers were soaked in a photocrosslinking solution (Irgacure 2959 dissolved in anhydrous ethanol at a concentration of 10% (w / v)) and irradiated with 365nm ultraviolet light for 100 minutes to induce crosslinking. Subsequently, the fibers were washed with anhydrous ethanol to remove excess photoinitiator, resulting in piezoelectric hydrogel nanofibers.

[0052] ②TA functional modified hydrogel nanofibers: dissolve tannic acid (TA, 15 mg / mL) in Tris-HCl (10 mM, pH 8.5) buffer to prepare a TA mixed solution; then soak the piezoelectric hydrogel nanofibers in the solution and gently oscillate at room temperature for 15 hours at an oscillation frequency of 150 rpm; take out the hydrogel nanofibers and wash them with ultrapure water several times to obtain a TA functional modified piezoelectric hydrogel fiber patch.

[0053] Example 3

[0054] ① GelMA nanofiber bundles were prepared by stable jet electrospinning technology: GelMA (substitution degree of 75%) was weighed and dissolved in HFIP solution to prepare a spinning solution with a concentration of 15% (w / v). In order to give GelMA hydrogel nanofibers piezoelectric properties, 20% (w / v) tetragonal barium titanate nanoparticles (T-BTO) were added to the spinning solution, and then ultrasonicated for 25 minutes to ensure uniform dispersion. The solution was stirred on a magnetic stirrer for more than 10 hours until a uniform solution was formed, and ultrasonicated again for 25 minutes before electrospinning. Piezoelectric nanofibers were then prepared based on the electrospinning method. The spinning parameters were: voltage 9-10kV, injection rate 20μL / min, receiving distance 20cm, drum speed 200rpm, and environmental conditions were maintained at 20-25°C and 25-30% humidity. To induce the formation of hydrogel nanofibers, dried T-BTO-added GelMA nanofibers were soaked in a photocrosslinking solution (Irgacure 2959 dissolved in anhydrous ethanol at a concentration of 15% (w / v)) and irradiated with 365nm ultraviolet light for 200 minutes to induce crosslinking. Subsequently, the fibers were washed with anhydrous ethanol to remove excess photoinitiator, resulting in piezoelectric hydrogel nanofibers.

[0055] ②TA functional modified hydrogel nanofibers: tannic acid (TA, 20 mg / mL) was dissolved in Tris-HCl (10 mM, pH 8.5) buffer to prepare a TA mixed solution; the piezoelectric hydrogel nanofibers were then immersed in the solution and gently oscillated at room temperature for 15 h at an oscillation frequency of 200 rpm; the hydrogel nanofibers were taken out and washed with ultrapure water several times to obtain a TA functional modified piezoelectric hydrogel fiber patch.

[0056] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. A method for preparing a motion-driven self-powered hydrogel nanofiber patch, characterized in that: The method comprises the following steps: (1) preparing a spinning solution, wherein the solute is GelMA and tetragonal barium titanate nanoparticles, and obtaining piezoelectric nanofibers after electrospinning; the degree of substitution of the GelMA is 10-60%; The addition amount of tetragonal barium titanate nanoparticles was 0.25% (w / v); (2) drying the piezoelectric nanofibers prepared in step (1), and then immersing the dried piezoelectric nanofibers in a photocrosslinking solution, crosslinking the piezoelectric nanofibers under ultraviolet irradiation, to obtain piezoelectric hydrogel nanofibers; (3) soaking the piezoelectric hydrogel nanofiber in a TA mixed solution, and taking it out after soaking to obtain a functionally modified piezoelectric hydrogel fiber patch, which is the motion-driven self-powered hydrogel nanofiber patch; The TA (tannic acid) mixed solution was prepared by dissolving tannic acid in Tris-HCl buffer at a concentration of 8 mg / mL.

2. The method for preparing the motion-driven self-powered hydrogel nanofiber patch according to claim 1, wherein: In step (1), the solvent of the spinning solution is HFIP, and the concentration of the spinning solution is 1-20% (w / v).

3. The method for preparing the motion-driven self-powered hydrogel nanofiber patch according to claim 1, wherein: In step (1), the electrospinning parameters were: voltage 9-10 kV, injection rate 20 μL / min, receiving distance 20 cm, drum speed 200 rpm, and the environmental conditions were maintained at 20-25°C and 25-30% humidity.

4. The method for preparing the motion-driven self-powered hydrogel nanofiber patch according to claim 1, wherein: In step (2), the photocrosslinking solution is prepared by dissolving a photoinitiator in anhydrous ethanol, and the concentration of the photocrosslinking agent solution is 1-20% (w / v); the photoinitiator is Irgacure 2959.

5. The method for preparing the motion-driven self-powered hydrogel nanofiber patch according to claim 1, wherein: In step (2), during the cross-linking treatment, 365 nm ultraviolet irradiation is performed for 1 to 300 min.

6. A motion-driven, self-powered hydrogel nanofiber patch prepared by the method according to any one of claims 1 to 5.

7. Use of the motion-driven self-powered hydrogel nanofiber patch according to claim 6 in the preparation of medical supplies.

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