Multifunctional low-temperature automatic storage system for biological samples

Abstract

The invention discloses a multifunctional low-temperature automatic preservation system for biological samples, and relates to the technical field of biological sample cryopreservation. A low-temperature storage cavity is formed in the heat preservation part, and a freezing storage set and a rotating assembly are placed in the low-temperature storage cavity. The transition part is provided with a transition area located around a taking and placing opening in the top of the heat preservation part, and a driving assembly is arranged in the transition area. A taking and placing cavity is formed in the heat preservation taking and placing part, and a bottom opening of the taking and placing cavity communicates with the top of the low-temperature storage cavity; the bottom of the lifting part is arranged at the top, located in the transition area, of the heat preservation tank body, a lifting assembly is arranged at the top of the lifting part, and a lifting part of the lifting assembly can sequentially penetrate through the heat preservation taking and placing part and the transition part and extend into the heat preservation part; the control part is used for controlling the rotating assembly to rotate through the driving assembly, used for horizontal linear movement of the lifting assembly and used for controlling vertical lifting of the lifting part. The low-temperature automatic storage of biological samples is realized, and the liquid nitrogen freezing frame automatically rotates; and the liquid nitrogen freezing frame is lifted in an auxiliary manner.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample freezing and preservation, and in particular to a multifunctional low-temperature automatic preservation system for biological samples. Background Art

[0002] Cryopreservation of biological samples refers to the use of cryogenic technology to freeze biological samples (such as cells, tissues, blood, sperm, eggs, embryos, etc.) and store them for a long time under appropriate conditions so that they can maintain their vitality or function after thawing in the future. Cryopreservation has a wide range of applications in medicine, agriculture, environmental protection, and scientific research. The basic principle of cryopreservation is to use low temperatures to slow down the biochemical reactions within and between cells to prevent the degradation of samples during storage. At the same time, freezing technology can also slow down the reproduction of pathogens and extend the shelf life of biological samples.

[0003] In the prior art, there is a biological sample freezing storage device and a biological sample moving method with a publication number of CN110654721B. The biological sample freezing storage device includes a storage container and a storage and access device fixed to the storage container. The storage container includes a tank body capable of containing a cryopreservative liquid and a container mouth located at the upper part of the tank body; and the storage and access device includes a column, a cross arm and a biological sample extraction part, the column is provided with a vertical moving part, the vertical moving part can move in the vertical direction along the column, the cross arm is provided with a horizontal moving part, the horizontal moving part can move in the horizontal direction along the cross arm; the cross arm is provided on the vertical moving part, so that the cross arm can move up and down in the vertical direction, and the biological sample extraction part is provided on the horizontal moving part, so that the biological sample extraction part can move in the horizontal direction.

[0004] However, the prior art cannot solve the problem of realizing the functions of automatic rotation of the liquid nitrogen freezing rack and auxiliary lifting of the liquid nitrogen freezing rack. Summary of the invention

[0005] In view of the above technical problems, the present application solves the problem in the prior art that the functions of automatic rotation of the liquid nitrogen freezing rack and auxiliary lifting of the liquid nitrogen freezing rack cannot be solved.

[0006] In order to achieve the above-mentioned purpose, the technical solution adopted in this application is: a multifunctional low-temperature automatic preservation system for biological samples, including a heat preservation part, a transition part, a heat preservation and placement part, a lifting part and a control part.

[0007] The heat preservation part has a low temperature storage cavity, in which a plurality of frozen storage groups for storing biological samples and a rotating assembly for driving the plurality of frozen storage groups to perform intermittent rotational motion are placed;

[0008] The transition part has a transition area around the top access opening of the heat preservation part, and a driving assembly for driving the rotating assembly is arranged in the transition area;

[0009] The heat-insulating access portion has an access cavity, and the bottom opening of the access cavity is connected to the top of the low-temperature storage cavity;

[0010] The bottom of the lifting part is arranged at the top of the heat preservation tank body in the transition area, and a lifting assembly that can be horizontally linearly moved is arranged on the top of the lifting part, and the lifting part of the lifting assembly can sequentially pass through the heat preservation taking and placing part and the transition part and extend into the heat preservation part;

[0011] The control unit is used to control the rotation of the rotating assembly through the driving assembly, to control the horizontal linear movement of the lifting assembly, and to control the vertical lifting of the lifting part.

[0012] In order to better realize the present invention, further, the heat preservation part includes a heat preservation tank body, the heat preservation tank body has a low-temperature storage cavity, the top of the low-temperature storage cavity is provided with a heat preservation tank body sampling port, and the heat preservation tank body sampling port is placed with a heat preservation plug cover B and a heat preservation plug cover A;

[0013] The rotating assembly includes a turntable and a rotating shaft, the turntable is arranged at the lower section of the rotating shaft, a frozen storage group is placed on the turntable, the bottom rotation of the rotating shaft is arranged at the center position of the insulation tank base at the bottom of the insulation tank body, and the top rotation of the rotating shaft is arranged at the top of the insulation tank body.

[0014] In order to better implement the present invention, further, the contact surface between the thermal insulation plug body cover B and the thermal insulation plug body cover A is a stepped surface.

[0015] In order to better implement the present invention, further, the freezing storage group includes a liquid nitrogen freezing rack, the area of ​​the heat preservation tank body located above the turntable forms a plurality of mutually independent freezing placement areas, each freezing placement area is placed with a plurality of the liquid nitrogen freezing racks, and a plurality of freezing boxes are vertically arranged on the liquid nitrogen freezing rack.

[0016] In order to better realize the present invention, further, the driving assembly includes a reducer, a chain, a driven wheel, a motor and a driving wheel.

[0017] The reducer and the motor are both arranged at the top of the heat-insulating tank body in the transition area, the input end of the reducer is connected to the output end of the motor, the output end of the reducer is provided with a driving wheel, and the top of the rotating shaft extending out of the heat-insulating tank body is provided with a driven wheel, and the driven wheel and the driving wheel are linked to each other by a chain.

[0018] In order to better realize the present invention, further, the heat-insulating access portion includes a cylinder, the cylinder has a access cavity, the top of the cylinder is provided with a cylinder top opening, a plug cover is placed on the cylinder top opening, a plug cover strip slit is provided in the middle of the plug cover, a spray assembly is provided on the inner wall surface of the upper section of the access cavity, and a access window assembly is provided in the middle and lower sections of the access cavity.

[0019] The spray assembly comprises a nozzle, an annular channel and a vertical channel. A plurality of output holes are provided in an annular shape at the lower side of the annular channel, each of which is provided with a nozzle. An input hole is provided at the upper side of the annular channel, and the input hole is communicated with the output end of the vertical channel.

[0020] The access window assembly includes an access door, a movable door, a strip observation hole, a limit guide strip, a vertical slide groove, a vertical slide block and a locking bolt. The access window is provided in the middle and lower sections of the access cavity. A connecting piece is provided on the outer peripheral wall surface of the cylinder above the access window. Limit guide strips are provided on the left and right sides of the access window.

[0021] The pick-and-place window is provided with a pick-and-place door that can be flipped to open and close, the movable door is located between the cylinder and the pick-and-place door, the left and right side edges of the movable door respectively correspond to and slide in contact with the inner side surface of the limiting guide strip, a vertical slide groove is vertically opened at the middle position of the movable door, the sliding sleeve in the vertical slide groove is provided with the connecting piece, the slide groove part of the vertical slide groove located above the connecting piece is provided with a vertical slider through a locking bolt sliding sleeve, and the vertical movement of the vertical slider can be locked by the locking bolt, and a strip-shaped observation hole is opened at the position of the movable door located below the vertical slide groove.

[0022] In order to better realize the present invention, further, the lifting part includes a lifting support frame, the insulation tank body of the lifting support frame is located at the top of the transition area, and a lifting assembly is arranged on the top of the lifting support frame, and the lifting assembly includes an aluminum plate, a slide trough, a guide rail, a strip aluminum profile, a lifting frame, a side slider, a rotating wheel, a lifting motor, a chain rope, a hook, a wire trough support frame, a tank chain wire trough, a ring chain and a driving motor.

[0023] The aluminum plate is fixedly arranged on the top of the lifting support frame, a slide groove is arranged on the upper surface of the aluminum plate, a guide rail is slidably arranged in the slide groove, a strip aluminum profile is arranged on the upper surface of the guide rail, a lifting frame is arranged at one end of the strip aluminum profile, a rotating wheel is arranged in the lifting frame to rotate via a first rotating shaft, one end of the first rotating shaft is connected to the lifting motor, one end of the chain lifting rope is arranged on the rotating wheel, and a hook is arranged at the other end of the chain lifting rope.

[0024] A wire trough support frame is provided on the upper surface of the aluminum plate on one side of the slide groove, the tank chain wire trough is provided on the upper surface of the wire trough support frame, a ring chain is provided in the tank chain wire trough, a sprocket is provided on the upper surface of the wire trough support frame away from the tank chain wire trough for rotation via a second rotating shaft, the part of the chain extending out of the tank chain wire trough is linked to the sprocket, and the drive motor is connected to one end of the second rotating shaft.

[0025] In order to better implement the present invention, the preservation system further includes a first liquid level sensor, a liquid inlet pipe, a first temperature sensor, a second liquid level sensor, a liquid replenishing valve, a liquid inlet valve, an exhaust valve and a second temperature sensor.

[0026] The first liquid level sensor, the liquid inlet pipe, the first temperature sensor and the second liquid level sensor are all located inside the heat preservation tank body, the first liquid level sensor and the liquid inlet pipe are both located close to the lower surface of the turntable, the first temperature sensor is located on the outer peripheral wall of the turntable, and the second liquid level sensor is located on the inner top of the heat preservation tank body;

[0027] The liquid replenishing valve, liquid inlet valve and exhaust valve are all located on the peripheral wall surface of the heat preservation tank body, the liquid inlet valve is connected to the liquid replenishing valve, the liquid replenishing valve is connected to the liquid inlet pipeline, the exhaust valve is connected between the liquid replenishing valve and the liquid inlet valve, and the exhaust valve is connected to the vertical channel;

[0028] The second temperature sensor is located in the cylinder;

[0029] The control unit includes a controller,

[0030] When the controller receives a signal that a designated frozen storage area is located below the sampling port of the heat preservation tank body, the controller sends a start signal to the first execution element that controls the opening and closing of the motor, so that the motor and the external power supply are in a conducting state, so that the turntable rotates; when the frozen storage area is located below the sampling port of the heat preservation tank body, the controller sends a shutdown signal to the first execution element that controls the opening and closing of the motor, so that the motor and the external power supply are in a disconnected state;

[0031] When the controller receives a signal for horizontally moving the lifting assembly, the controller sends a start signal to the second execution element that controls the opening and closing of the driving motor of the lifting assembly, so that the driving motor and the external power supply are in a conducting state, so that the lifting assembly moves horizontally; when the lifting assembly moves to a specified position, the controller sends a shutdown signal to the second execution element that controls the opening and closing of the driving motor of the lifting assembly, so that the driving motor and the external power supply are in a disconnected state;

[0032] When the controller receives a signal that causes the hook of the lifting assembly to be raised or lowered, the controller sends a start signal to the third execution element that controls the opening and closing of the lifting motor of the lifting assembly, so that the lifting motor is in a conductive state with the external power supply, so that the hook of the lifting motor rises or falls; when the hook of the lifting motor rises or falls to a specified position, the controller sends a shutdown signal to the third execution element that controls the opening and closing of the lifting motor, so that the lifting or falling of the hook of the lifting motor is disconnected from the external power supply;

[0033] The controller receives a first liquid level signal detected by the first liquid level sensor, and converts the first liquid level signal into a first digital signal. When the first digital signal is less than a preset liquid replenishment starting value, the controller sends a first control signal to the liquid replenishment valve and the liquid inlet valve, and the liquid replenishment valve and the liquid inlet valve are opened, so that the external liquid is replenished to the bottom of the heat preservation tank body through the liquid inlet pipe;

[0034] The controller receives a first temperature signal or a second temperature signal corresponding to the first temperature sensor or the second temperature sensor, and converts the first temperature signal or the second temperature signal into a second digital signal or a third digital signal respectively. When one of the second digital signal or the third digital signal is greater than the exhaust setting value preset in the corresponding control unit, the controller sends a second control signal to the liquid inlet valve and the exhaust valve, and the liquid inlet valve is closed and the exhaust valve is opened; otherwise, the controller sends a third control signal to the liquid inlet valve and the exhaust valve, and the liquid replenishing valve and the liquid inlet valve are opened, and the exhaust valve is closed.

[0035] The controller receives a second liquid level signal detected by the first liquid level sensor and converts the second liquid level signal into a second digital signal. When the second digital signal is greater than a preset liquid replenishment stop value, the controller sends a fourth control signal to the liquid replenishment valve, the liquid inlet valve and the exhaust valve, and the liquid replenishment valve, the liquid inlet valve and the exhaust valve are closed.

[0036] In order to better implement the present invention, further, the preservation system also includes a laser locator, and the laser locator is arranged on one side of the lifting frame.

[0037] In order to better implement the present invention, further, a step frame is provided on one side of the bottom of the heat-insulating tank body, and an endoscope is placed on the top of the heat-insulating tank body in the transition area.

[0038] Compared with the prior art, the technical solution provided by the present invention has the following beneficial effects:

[0039] 1. The present invention realizes the automatic low-temperature preservation of biological samples through the coordinated design of the heat preservation part, the transition part, the heat preservation and placement part, the lifting part and the control part. On the one hand, it does not need to manually rotate the liquid nitrogen freezing rack to place the tray; the internal spray cooling of the heat preservation box keeps the cryobox at -80℃~

[0040] The low temperature area of ​​-170℃ keeps the samples in a protected state at all times; it also assists in lifting the liquid nitrogen freezing rack.

[0041] 2. The present invention rotates the freezing placement area through the coordinated design of the driving component and the rotating component, so that the liquid nitrogen freezing rack where the pre-taken biological sample is located is rotated to just below the sampling port of the insulation tank body, which is convenient for taking and placing.

[0042] 3. The present invention facilitates taking by designing the contact surface between the thermal insulation plug body cover B and the thermal insulation plug body cover A as a stepped surface.

[0043] 4. The present invention is adapted to different biological samples by designing different freezing placement areas.

[0044] 5. The present invention provides a larger space for the heat preservation tank body by locating the drive assembly in the transition area.

[0045] 6. The present invention ensures that the biological sample remains in a heat-insulating state during the process of taking and placing by designing the cylinder, the taking and placing door, the top opening of the cylinder, the plug cover, the strip slit of the plug cover, the movable door, the strip observation hole, the limit guide strip, the nozzle, the annular channel, the vertical channel, the vertical slide groove, the vertical slide block and the locking bolt.

[0046] 7. The present invention assists operators in extracting the liquid nitrogen freezing rack through the coordinated design of the lifting part.

[0047] 8. The present invention ensures, through the design of the control unit, on the one hand, the automatic rotation of the liquid nitrogen freezing rack in the insulation unit, the horizontal sliding of the lifting unit, and the automatic lifting and lowering of the lifting assembly in the lifting unit; on the other hand, the automatic implementation of liquid inlet, liquid replenishment, and exhaust is ensured.

[0048] 9. The present invention uses a laser locator designed to be used in conjunction with a lifting part to locate the top lifting position of the liquid nitrogen freezing rack in a mist state, making it convenient to hang it with a hook.

[0049] 10. The present invention provides a sufficient height for the operator to operate at the movable door through the design of the ladder rack. The design of the endoscope, based on the laser locator, further facilitates the operator to identify the required position of the liquid nitrogen freezing rack. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative labor.

[0051] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0052] Figure 2 for Figure 1 A front view of

[0053] Figure 3 for Figure 2 Sectional view at AA;

[0054] Figure 4 for Figure 1 The schematic diagram of the structure of the cylinder in the heat preservation taking and placing part is removed;

[0055] Figure 5 for Figure 4 Sectional view at the middle BB;

[0056] Figure 6 for Figure 1 A three-dimensional schematic diagram of the cylinder in the heat preservation and placement part is removed

[0057] Figure 7 for Figure 6 Enlarged view of point C in the middle;

[0058] Figure 8 This is a schematic diagram of the structure without the middle part of the insulation tank;

[0059] Fig. 9 for Figure 8 The enlarged view of point D in the middle;

[0060] Fig.10 It is a schematic diagram of the structure of the plug body cover in the present invention;

[0061] Fig.11 It is a schematic diagram of the cooperation between the thermal insulation plug body cover A and the thermal insulation plug body cover B in the present invention;

[0062] Fig.12 It is a schematic diagram of the structure of the liquid nitrogen freezing rack in the present invention.

[0063] In the figure: 10-insulation part; 20-transition part; 30-insulation taking and placing part; 40-lifting part; 50-control part; 101-insulation tank body; 102-insulation tank body sampling port; 103-insulation plug body cover B; 104-insulation plug body cover A; 105-insulation tank body base; 106-turntable; 107-liquid nitrogen freezing rack; 108-first liquid level sensor; 109-liquid inlet pipeline; 110-first temperature sensor; 111-second liquid level sensor; 112-liquid replenishing valve; 113-liquid inlet valve; 114-exhaust valve; 115-rotating shaft; 116-ladder rack; 2 01-reducer; 202-chain; 203-driven wheel; 204-motor; 205-driving wheel; 301-cylinder; 302-pick-and-place door; 303-top opening of cylinder; 304-plug cover; 305-slit of plug cover; 306-movable door; 307-strip observation hole; 308-limiting guide strip; 309-nozzle; 310-annular channel; 311-vertical channel; 312-vertical slide groove; 313-vertical slider; 314-locking bolt; 315-connecting plate; 401-lifting support frame; 402-chain lifting rope; 501-endoscope. DETAILED DESCRIPTION

[0064] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0065] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for which protection is sought, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present application.

[0066] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0067] In the description of this application, it should be noted that if the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the drawings, or the orientation or position relationship in which the product of the application is usually placed when in use. It is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation on this application. In addition, if the terms "first", "second", etc. appear in the description of this application, they are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0068] In addition, if the terms "horizontal" or "vertical" appear in the description of this application, it does not mean that the components are required to be absolutely horizontal or suspended, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0069] In the description of this application, it should also be noted that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0070] Embodiment 1

[0071] like Figure 1 As shown, a multifunctional low-temperature automatic preservation system for biological samples includes a heat preservation part 10, a transition part 20, a heat preservation taking and placing part 30, a lifting part 40 and a control part 50.

[0072] The heat preservation part 10 has a low temperature storage cavity in which a plurality of frozen storage groups for storing biological samples and a rotating assembly for driving the plurality of frozen storage groups to perform intermittent rotational motion are placed;

[0073] The transition part 20 has a transition area around the top access opening of the heat preservation part 10, and a driving component for driving the rotating component is arranged in the transition area;

[0074] The heat-insulating access portion 30 has an access cavity therein, and the bottom opening of the access cavity is connected to the top of the low-temperature storage cavity;

[0075] The bottom of the lifting part 40 is arranged at the top of the heat preservation tank body 101 in the transition area, and a lifting assembly that can be horizontally linearly moved is arranged on the top of the lifting part 40, and the lifting part of the lifting assembly can sequentially pass through the heat preservation taking and placing part 30 and the transition part 20 and extend into the heat preservation part 10;

[0076] The control unit 50 is used to control the rotation of the rotating assembly through the driving assembly, to control the horizontal linear movement of the lifting assembly, and to control the vertical lifting of the lifting part.

[0077] Through the coordinated design of the heat preservation part 10, the transition part 20, the heat preservation taking and placing part 30, the lifting part 40 and the control part 50, on the one hand, the low-temperature automatic preservation of the biological sample is realized; on the other hand, there is no need to manually rotate the liquid nitrogen freezing rack 107 to place the tray; the internal spray cooling of the heat preservation box keeps the cryobox in the low temperature area of ​​-80°C to -170°C during the storage and retrieval process, so that the sample is always in a protected state; and the liquid nitrogen freezing rack is assisted in lifting.

[0078] like Figure 1 , Figure 2 , Figure 4 , Figure 5 , Figure 6 and Figure 8 As shown, in this embodiment, the heat preservation part 10 includes a heat preservation tank body 101, and the heat preservation tank body 101 has a low-temperature storage cavity. The top of the low-temperature storage cavity is provided with a heat preservation tank body sampling port 102, and the heat preservation tank body sampling port 102 is placed with a heat preservation plug cover B103 and a heat preservation plug cover A104;

[0079] The rotating assembly includes a turntable 106 and a rotating shaft 115. The turntable 106 is arranged at the lower section of the rotating shaft 115. A frozen storage group is placed on the turntable 106. The bottom rotation of the rotating shaft 115 is arranged at the center position of the insulation tank base 105 at the bottom of the insulation tank body 101, and the top rotation of the rotating shaft 115 is arranged at the top of the insulation tank body 101.

[0080] By means of the coordinated design of the driving assembly and the rotating assembly, the freezing placement area is rotated so that the liquid nitrogen freezing rack 107 where the pre-taken biological sample is located is rotated to the position directly below the sampling port 102 of the insulation tank body, which is convenient for taking and placing.

[0081] like Figure 8 and Fig.11 As shown, in this embodiment, the contact surface between the thermal insulation plug body cover B103 and the thermal insulation plug body cover A104 is a stepped surface.

[0082] The contact surface between the thermal insulation plug body cover B103 and the thermal insulation plug body cover A104 is designed as a stepped surface, which is convenient for taking.

[0083] like Figure 2 , Figure 5 , Figure 6 , Figure 8 and Fig.12 As shown, in this embodiment, the freezing storage group includes a liquid nitrogen freezing rack 107, and the area of ​​the heat preservation tank body 101 located above the turntable 106 forms a plurality of mutually independent freezing placement areas, each freezing placement area is placed with a plurality of the liquid nitrogen freezing racks 107, and a plurality of freezing boxes are vertically arranged on the liquid nitrogen freezing rack 107.

[0084] By designing different freezing storage areas, it can adapt to different biological samples.

[0085] like Figure 5 As shown, in this embodiment, the driving assembly includes a reducer 201, a chain 202, a driven wheel 203, a motor 204 and a driving wheel 205.

[0086] The reducer 201 and the motor 204 are both arranged at the top of the heat-insulating tank body 101 in the transition area, the input end of the reducer 201 is connected to the output end of the motor 204, the output end of the reducer 201 is provided with a driving wheel 205, and the top of the rotating shaft 115 extending out of the heat-insulating tank body 101 is provided with a driven wheel 203, and the driven wheel 203 and the driving wheel 205 are linked to each other by a chain 202.

[0087] The design of locating the driving assembly in the transition area allows the heat-insulating tank body 101 to have a larger space.

[0088] like Figure 1 , Figure 3 , Figure 4 , Figure 6 , Figure 7 , Figure 8 As shown in FIG. 10 , in this embodiment, the heat-insulating taking-and-placing part 30 comprises a barrel 301, the barrel 301 having a taking-and-placing cavity, the top of the barrel 301 is provided with a barrel top opening 303, a plug cover 304 is placed on the barrel top opening 303, a plug cover strip slit 305 is provided in the middle of the plug cover 304, a spray assembly is provided on the inner wall surface of the upper section of the taking-and-placing cavity, and a taking-and-placing window assembly is provided in the middle and lower sections of the taking-and-placing cavity,

[0089] The spray assembly includes a nozzle 309, an annular channel 310 and a vertical channel 311. A plurality of output holes are provided in an annular shape at the lower side of the annular channel 310, each of which is provided with a nozzle 309. An input hole is provided at the upper side of the annular channel 310, and the input hole is communicated with the output end of the vertical channel 311.

[0090] The access window assembly includes an access door 302, a movable door 306, a strip observation hole 307, a limit guide strip 308, a vertical slide groove 312, a vertical slide block 313 and a locking bolt 314. The access window is provided in the middle and lower sections of the access cavity. A connecting piece 315 is provided on the outer peripheral wall surface of the cylinder 301 above the access window. The limit guide strips 308 are provided on the left and right sides of the access window.

[0091] The pick-and-place window is provided with a pick-and-place door 302 that can be flipped to open and close, and the movable door 306 is located between the cylinder 301 and the pick-and-place door 302. The left and right side edges of the movable door 306 correspond to and slide in contact with the inner side surface of the limiting guide strip 308 respectively, and a vertical slide groove 312 is vertically opened at the middle position of the movable door 306. The connecting piece 315 is slidably sleeved in the vertical slide groove 312. The slide groove part of the vertical slide groove 312 located above the connecting piece 315 is slidably sleeved with a vertical slider 313 through a locking bolt 314, and the vertical movement of the vertical slider 313 can be locked by the locking bolt 314. A strip-shaped observation hole 307 is opened at the position of the movable door 306 located below the vertical slide groove 312.

[0092] Through the design of the cylinder 301, the taking and placing door 302, the top opening 303 of the cylinder, the plug cover 304, the plug cover strip slit 305, the movable door 306, the strip observation hole 307, the limiting guide strip 308, the nozzle 309, the annular channel 310, the vertical channel 311, the vertical slide groove 312, the vertical slide block 313 and the locking bolt 314, it is ensured that the biological sample remains in a heat-insulating state during the taking and placing process.

[0093] like Figure 1 , Figure 2 and Figure 8 As shown, in this embodiment, the lifting part 40 includes a lifting support frame 401, and the heat preservation tank body 101 of the lifting support frame 401 is located at the top of the transition area. A lifting component is arranged on the top of the lifting support frame 401, and the lifting component includes an aluminum plate, a slide groove, a guide rail, a strip aluminum profile, a lifting frame, a side slider, a rotating wheel, a lifting motor, a chain rope 402, a hook, a wire trough support frame, a tank chain wire trough, a ring chain and a driving motor.

[0094] The aluminum plate is fixedly arranged on the top of the lifting support frame 401, a slide groove is arranged on the upper surface of the aluminum plate, a guide rail is slidably arranged in the slide groove, a strip aluminum profile is arranged on the upper surface of the guide rail, a lifting frame is arranged at one end of the strip aluminum profile, a rotating wheel is arranged in the lifting frame to rotate through a first rotating shaft, one end of the first rotating shaft is connected to the lifting motor, one end of the chain lifting rope 402 is arranged on the rotating wheel, and a hook is arranged at the other end of the chain lifting rope 402.

[0095] A wire trough support frame is provided on the upper surface of the aluminum plate on one side of the slide groove, the tank chain wire trough is provided on the upper surface of the wire trough support frame, a ring chain is provided in the tank chain wire trough, a sprocket is provided on the upper surface of the wire trough support frame away from the tank chain wire trough for rotation via a second rotating shaft, the part of the chain extending out of the tank chain wire trough is linked to the sprocket, and the drive motor is connected to one end of the second rotating shaft.

[0096] The coordinated design of the lifting portion 40 assists the operator in extracting the liquid nitrogen freezing rack 107 .

[0097] like Figure 8 and Fig. 9 As shown, in this embodiment, the preservation system also includes a first liquid level sensor 108, a liquid inlet pipe 109, a first temperature sensor 110, a second liquid level sensor 111, a liquid replenishing valve 112, a liquid inlet valve 113, an exhaust valve 114 and a second temperature sensor.

[0098] The first liquid level sensor 108, the liquid inlet pipe 109, the first temperature sensor 110 and the second liquid level sensor 111 are all located inside the heat preservation tank body 101. The first liquid level sensor 108 and the liquid inlet pipe 109 are both located close to the lower surface of the turntable 106 (the distance between the liquid inlet pipe 109 and the lower surface of the turntable 106 is 10 mm to 50 mm). The first temperature sensor 110 is located at the outer peripheral wall of the turntable 106. The second liquid level sensor 111 is located at the inner top of the heat preservation tank body 101.

[0099] The liquid replenishing valve 112, the liquid inlet valve 113 and the exhaust valve 114 are all located on the outer peripheral wall of the heat-insulating tank body 101, the liquid inlet valve 113 is in communication with the liquid replenishing valve 112, the liquid replenishing valve 112 is in communication with the liquid inlet pipe 109, the exhaust valve 114 is in communication between the liquid replenishing valve 112 and the liquid inlet valve 113, and the exhaust valve 114 is in communication with the vertical channel 311;

[0100] The second temperature sensor is located in the cylinder 301;

[0101] The control unit 50 includes a controller,

[0102] When the controller receives a signal that specifies a frozen storage area to be located below the sampling port 102 of the heat preservation tank, the controller sends a start signal to a first execution element that controls the opening and closing of the motor 204 (for example, a first relay that conducts between the motor 204 and an external power source), so that the motor 204 and the external power source are in a conducting state, so that the turntable 106 is rotated; when the frozen storage area is located below the sampling port 102 of the heat preservation tank, the controller sends a shutdown signal to the first execution element that controls the opening and closing of the motor 204, so that the motor 204 and the external power source are in a disconnected state;

[0103] When the controller receives a signal for horizontally moving the lifting assembly, the controller sends a start signal to a second execution element (for example, a second relay between the drive motor and an external power source) that controls the opening and closing of the drive motor of the lifting assembly, so that the drive motor and the external power source are in a conducting state, so that the lifting assembly moves horizontally; when the lifting assembly moves to a specified position, the controller sends a shutdown signal to the second execution element that controls the opening and closing of the drive motor of the lifting assembly, so that the drive motor and the external power source are in a disconnected state;

[0104] When the controller receives a signal causing the hook of the lifting assembly to be raised or lowered, the controller sends a start signal to a third execution element that controls the opening and closing of the lifting motor of the lifting assembly (for example, turning on a third relay between the lifting motor and an external power supply), so that the lifting motor and the external power supply are in a conducting state, so that the hook of the lifting motor rises or falls; when the hook of the lifting motor rises or falls to a specified position, the controller sends a shutdown signal to the third execution element that controls the opening and closing of the lifting motor, so that the lifting or falling of the hook of the lifting motor is disconnected from the external power supply;

[0105] The controller receives a first liquid level signal detected by the first liquid level sensor 108, and converts the first liquid level signal into a first digital signal. When the first digital signal is less than a preset liquid replenishment starting value, the controller sends a first control signal to the liquid replenishment valve 112 and the liquid inlet valve 113, and the liquid replenishment valve 112 and the liquid inlet valve 113 are opened, so that the external liquid is replenished to the bottom of the thermal insulation tank body 101 through the liquid inlet pipe 109;

[0106] The controller receives a first temperature signal or a second temperature signal corresponding to the first temperature sensor 110 or the second temperature sensor, and converts the first temperature signal or the second temperature signal into a second digital signal or a third digital signal respectively. When one of the second digital signal or the third digital signal is greater than the exhaust setting value preset in the corresponding control unit, the controller sends a second control signal to the liquid inlet valve 113 and the exhaust valve 114, and the liquid inlet valve 113 is closed and the exhaust valve 114 is opened; otherwise, the controller sends a third control signal to the liquid inlet valve 113 and the exhaust valve 114, and the liquid replenishing valve 112 and the liquid inlet valve 113 are opened, and the exhaust valve 114 is closed.

[0107] The controller receives a second liquid level signal detected by the first liquid level sensor 108, and converts the second liquid level signal into a second digital signal. When the second digital signal is greater than a preset liquid replenishment stop value, the controller sends a fourth control signal to the liquid replenishment valve 112, the liquid inlet valve 113 and the exhaust valve 114, and the liquid replenishment valve 112, the liquid inlet valve 113 and the exhaust valve 114 are closed.

[0108] Through the design of the control unit 50, on the one hand, the automatic rotation of the liquid nitrogen freezing rack 107 in the insulation unit 10 is ensured, the horizontal sliding of the lifting unit 40 and the automatic lifting of the lifting assembly in the lifting unit 40 are ensured; on the other hand, the automatic implementation of liquid inlet, liquid replenishment and exhaust is ensured.

[0109] In this embodiment, the preservation system also includes a laser locator, and the laser locator is arranged on one side of the lifting frame.

[0110] Through the design of the laser locator, used in conjunction with the lifting part 40, the top lifting position of the liquid nitrogen freezing rack 107 is located in the mist state, which is convenient for hanging with the hook.

[0111] like Figure 1 As shown, in this embodiment, a step frame 116 is further provided on one side of the bottom of the heat-insulating tank body 101, and an endoscope 501 is also placed on the top of the heat-insulating tank body 101 in the transition area.

[0112] The design of the step rack 116 provides the operator with a sufficient height to operate at the movable door 306. The design of the endoscope 501, on the basis of the laser locator, further facilitates the operator to identify the required position of the liquid nitrogen freezing rack 107.

[0113] In addition, the control unit 50 is further provided with a touch screen, and the touch screen is connected to the controller.

[0114] Working principle:

[0115] Automatic mode rehydration can be achieved:

[0116] Operations before starting automatic refilling: pre-set the refilling start value and refilling stop value;

[0117] After the settings are completed, click [Automatic Mode] to turn on the automatic refilling mode through the control of the controller;

[0118] When the liquid level is lower than the starting value of refilling, the automatic refilling mode is turned on.

[0119] STEP 1: When the liquid level is lower than the starting value of liquid replenishment, open the [liquid inlet valve];

[0120] STEP2: When the exhaust temperature is higher than the exhaust setting value, open the exhaust valve to enter the exhaust process;

[0121] The time / temperature of the exhaust process can be set as required in the parameter settings;

[0122] When the time is reached or the exhaust temperature is ≤ the set exhaust temperature, the exhaust process ends;

[0123] STEP3: After the exhaust process is completed, close the exhaust valve and open the refill valve to enter the refill process;

[0124] When the rehydration stop value is reached, rehydration stops. The rehydration start and stop values ​​are set in the parameter settings.

[0125] Effect achieved: Automatic refilling can be achieved in automatic mode, and refilling is performed automatically as liquid nitrogen is consumed.

[0126] When defogging, liquid nitrogen is injected into the heat-insulating tank body 101 or sprayed through the nozzle 309 .

[0127] During gas phase storage, if a malfunction or accident causes the liquid nitrogen storage volume to exceed the lowest frozen sample position (i.e., exceeds the height of the turntable 106),

[0128] The protection program is started to stop the automatic filling of liquid nitrogen and start the alarm (the control unit also includes an alarm device, which is connected to the controller).

[0129] Conditions to determine if liquid nitrogen is too high:

[0130] Condition 1: Liquid level height > set maximum liquid level height value;

[0131] Condition 2: The lower temperature exceeds the lower limit of the lower temperature -195°C;

[0132] When one of the above conditions is triggered, all valves are forced to close, and the inlet valve and the replenishing valve cannot be opened manually or automatically.

[0133] Preset the insulation chamber refrigeration start temperature and insulation chamber refrigeration shut-off temperature;

[0134] Then click the "Insulation Chamber Cooling" button on the control screen homepage;

[0135] After the insulation chamber refrigeration is turned on, the following control process can also be performed:

[0136] Step 1: First open the [liquid inlet valve] + [exhaust valve] to exhaust air.

[0137] Step 2: After the exhaust temperature is reached, close the exhaust valve; open the insulation chamber refrigeration solenoid valve (hereinafter referred to as the refrigeration valve), and then determine whether the main tank liquid level needs to be replenished; if the main tank liquid level is above the set [replenishment start value], do not open the replenishment valve:

[0138] If it is not above the refilling start value, the tank body is triggered to automatically refill (i.e., the refilling valve of the main tank is opened, and the refilling valve is closed after the refilling stop value is reached).

[0139] Step 3: As liquid nitrogen is sprayed, the temperature of the insulation chamber decreases. When the temperature of the insulation chamber reaches the set value (insulation chamber refrigeration shut-off temperature), the refrigeration valve is closed after the delayed spraying delay time (seconds):

[0140] Step 4: After the refrigeration valve is closed, as the temperature of the insulation chamber rises to above the refrigeration opening temperature of the insulation chamber, the refrigeration valve is opened again to spray: maintain the temperature in the insulation chamber.

[0141] Step 5: Until the user opens the door of the insulation chamber (door switch signal), or closes the insulation chamber on the screen

[0142] The refrigeration button stops the above refrigeration and heat preservation process, resets all solenoid valves, and opening the door will reset the refrigeration button of the heat preservation chamber.

[0143] Sample access operation flow

[0144] 1. Manually open the insulation box door (i.e., the insulation box is the cylinder 301, and the insulation box door is the access door 302 and the movable door 306 at the access window), the box opening size is 275mm wide * 4 insulation access parts 30mm high.

[0145] (Freezing rack height about 660mm)

[0146] 2. Manually remove the large-neck plug cover, which is divided into two parts: A and B. Remove the A and B covers in order.

[0147] 3. Click the access button on the touch screen to select a storage area (or a frozen storage area, for example, frozen storage area A, frozen storage area B, frozen storage area C, frozen storage area D), so that a frozen storage area is rotated to the bottom of the sampling port 102 of the insulation tank body.

[0148] 4. Press and hold the lifting and lowering button (physical button) to lower the lifting hook to the appropriate position, slide the lifting hook back and forth to the freezing rack position and hang the lifting hook. (The height distance between the top of the freezing rack and the sampling port is about 580mm).

[0149] 5. Close the incubator door

[0150] Set the required temperature for insulation on the touch screen and click the cooling button for cooling.

[0151] After reaching the refrigeration temperature, click the touch screen to turn off the refrigeration button and open the insulation box door (the controller is also connected to a refrigeration solenoid valve, which is forced to close after the door is opened, which is an existing mature technical means).

[0152] 7. Press the lifting button (physical button) to raise the lifting hook to the sampling door.

[0153] Manually take out and put in the cryopreservation boxes. (The cryopreservation boxes need to be manually found on which layer of the cryopreservation rack)

[0154] 8. After sampling, return the cryopreservation rack to the storage area, cover it with the wide-necked plug cap, and place it in order.

[0155] Put on covers B and A.

[0156] 9. Close the insulation box door, and the entire sampling process is completed.

[0157] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A multifunctional low-temperature automatic preservation system for biological samples, characterized in that: It comprises a heat preservation part (10), a transition part (20), a heat preservation taking and placing part (30), a lifting part (40) and a control part (50), The heat preservation part (10) has a low-temperature storage cavity, in which a plurality of frozen storage groups for storing biological samples and a rotating assembly for driving the plurality of frozen storage groups to perform intermittent rotational motion as a whole are placed; The transition part (20) has a transition area located around the top access opening of the heat preservation part (10), and a driving component for driving the rotating component is arranged in the transition area; The heat-insulating access portion (30) has an access cavity therein, and the bottom opening of the access cavity is connected to the top of the low-temperature storage cavity; The bottom of the lifting part (40) is arranged at the top of the heat-insulating tank body (101) located in the transition area, and a lifting assembly that can be horizontally linearly moved is arranged on the top of the lifting part (40), and the lifting part of the lifting assembly can sequentially pass through the heat-insulating taking and placing part (30), the transition part (20) and extend into the heat-insulating part (10); The control unit (50) is used to control the rotation of the rotating assembly through the driving assembly, to control the horizontal linear movement of the lifting assembly, and to control the vertical lifting of the lifting part.

2. A multifunctional low-temperature automatic preservation system for biological samples according to claim 1, characterized in that: The heat preservation part (10) comprises a heat preservation tank body (101), wherein the heat preservation tank body (101) has a low-temperature storage cavity, a heat preservation tank body sampling port (102) is provided at the top of the low-temperature storage cavity, and a heat preservation tank body sampling port (102) is placed at the heat preservation tank body sampling port (102); a heat preservation plug body cover B (103) and a heat preservation plug body cover A (104) are placed at the heat preservation tank body sampling port (102); The rotating assembly comprises a turntable (106) and a rotating shaft (115), wherein the turntable (106) is arranged at the lower section of the rotating shaft (115), a frozen storage group is placed on the turntable (106), the bottom of the rotating shaft (115) is rotatably arranged at the center position of the heat preservation tank body base (105) at the bottom of the heat preservation tank body (101), and the top of the rotating shaft (115) is rotatably arranged at the top of the heat preservation tank body (101).

3. A multifunctional low-temperature automatic preservation system for biological samples according to claim 2, characterized in that: The contact surface between the thermal insulation plug body cover B (103) and the thermal insulation plug body cover A (104) is a stepped surface.

4. The multifunctional low-temperature automatic preservation system for biological samples according to claim 2, characterized in that: The freezing storage group comprises a liquid nitrogen freezing rack (107), the area of ​​the heat-insulating tank body (101) located above the turntable (106) forms a plurality of mutually independent freezing placement areas, each freezing placement area is provided with a plurality of the liquid nitrogen freezing racks (107), and a plurality of freezing boxes are vertically arranged on the liquid nitrogen freezing rack (107).

5. A multifunctional low-temperature automatic preservation system for biological samples according to claim 4, characterized in that: The driving assembly comprises a reducer (201), a chain (202), a driven wheel (203), a motor (204) and a driving wheel (205). The reducer (201) and the motor (204) are both arranged at the top of the heat-insulating tank body (101) located in the transition area, the input end of the reducer (201) is connected to the output end of the motor (204), the output end of the reducer (201) is provided with a driving wheel (205), the top of the rotating shaft (115) extending out of the heat-insulating tank body (101) is provided with a driven wheel (203), and the driven wheel (203) and the driving wheel (205) are linked to each other by a chain (202).

6. A multifunctional low-temperature automatic preservation system for biological samples according to claim 5, characterized in that: The heat-insulating access portion (30) comprises a cylinder (301), the cylinder (301) having an access cavity, a cylinder top opening (303) being provided at the top of the cylinder (301), a plug cover (304) being placed on the cylinder top opening (303), a plug cover strip slit (305) being provided at the middle of the plug cover (304), a spray assembly being provided on the inner wall surface of the upper section of the access cavity, and a access window assembly being provided at the middle and lower sections of the access cavity. The spray assembly comprises a nozzle (309), an annular channel (310) and a vertical channel (311); a plurality of output holes are provided in an annular shape on the lower side of the annular channel (310); each output hole is provided with a nozzle (309); an input hole is provided on the upper side of the annular channel (310); the input hole is communicated with the output end of the vertical channel (311); The access window assembly comprises an access door (302), a movable door (306), a strip-shaped observation hole (307), a limiting guide strip (308), a vertical slide groove (312), a vertical slide block (313) and a locking bolt (314); a access window is provided in the middle and lower sections of the access cavity; a connecting piece (315) is provided on the outer peripheral wall surface of the cylinder (301) above the access window; and limiting guide strips (308) are provided on the left and right sides of the access window; The pick-and-place window is provided with a pick-and-place door (302) that can be flipped open and closed, the movable door (306) is located between the cylinder (301) and the pick-and-place door (302), the left and right side edges of the movable door (306) respectively correspond to the inner side surface of the limiting guide strip (308) for sliding fit, a vertical slide groove (312) is vertically opened at the middle position of the movable door (306), the connecting piece (315) is slidably sleeved in the vertical slide groove (312), the slide groove part of the vertical slide groove (312) located above the connecting piece (315) is slidably sleeved with a vertical slider (313) through a locking bolt (314), and the vertical movement of the vertical slider (313) can be locked by the locking bolt (314), and a strip-shaped observation hole (307) is opened at the position of the movable door (306) located below the vertical slide groove (312).

7. A multifunctional low-temperature automatic preservation system for biological samples according to claim 6, characterized in that: The lifting part (40) includes a lifting support frame (401), the lifting support frame (401) and the heat preservation tank body (101) are located at the top of the transition area, and a lifting component is arranged on the top of the lifting support frame (401), and the lifting component includes an aluminum plate, a slide groove, a guide rail, a strip aluminum profile, a lifting frame, a side slider, a rotating wheel, a lifting motor, a chain rope (402), a hook, a wire trough support frame, a tank chain wire trough, a ring chain and a driving motor. The aluminum plate is fixedly arranged on the top of the lifting support frame (401), a slide groove is arranged on the upper surface of the aluminum plate, a guide rail is slidably arranged in the slide groove, a strip aluminum profile is arranged on the upper surface of the guide rail, a lifting frame is arranged at one end of the strip aluminum profile, a rotating wheel is arranged in the lifting frame for rotation via a first rotating shaft, one end of the first rotating shaft is connected to the lifting motor, one end of the chain lifting rope (402) is arranged on the rotating wheel, and a hook is arranged at the other end of the chain lifting rope (402). A wire trough support frame is provided on the upper surface of the aluminum plate on one side of the slide groove, the tank chain wire trough is provided on the upper surface of the wire trough support frame, a ring chain is provided in the tank chain wire trough, a sprocket is provided on the upper surface of the wire trough support frame away from the tank chain wire trough for rotation via a second rotating shaft, the part of the chain extending out of the tank chain wire trough is linked to the sprocket, and the drive motor is connected to one end of the second rotating shaft.

8. The multifunctional low-temperature automatic preservation system for biological samples according to claim 7, characterized in that: The preservation system also includes a first liquid level sensor (108), a liquid inlet pipeline (109), a first temperature sensor (110), a second liquid level sensor (111), a liquid replenishing valve (112), a liquid inlet valve (113), an exhaust valve (114) and a second temperature sensor. The first liquid level sensor (108), the liquid inlet pipe (109), the first temperature sensor (110) and the second liquid level sensor (111) are all located inside the heat-insulating tank body (101); the first liquid level sensor (108) and the liquid inlet pipe (109) are both located close to the lower surface of the rotating disk (106); the first temperature sensor (110) is located on the outer peripheral wall surface of the rotating disk (106); and the second liquid level sensor (111) is located on the inner top of the heat-insulating tank body (101); The liquid replenishing valve (112), the liquid inlet valve (113) and the exhaust valve (114) are all located on the outer peripheral wall of the heat-insulating tank body (101); the liquid inlet valve (113) is in communication with the liquid replenishing valve (112); the liquid replenishing valve (112) is in communication with the liquid inlet pipeline (109); the exhaust valve (114) is in communication between the liquid replenishing valve (112) and the liquid inlet valve (113); and the exhaust valve (114) is in communication with the vertical channel (311); The second temperature sensor is located in the cylinder (301); The control unit (50) includes a controller, When the controller receives a signal that specifies a frozen storage area to be located below the sampling port (102) of the heat preservation tank body, the controller sends a start signal to the first execution element that controls the opening and closing of the motor (204), so that the motor (204) and the external power supply are in a conducting state, thereby rotating the turntable (106); when the frozen storage area is located below the sampling port (102) of the heat preservation tank body, the controller sends a shut-down signal to the first execution element that controls the opening and closing of the motor (204), so that the motor (204) and the external power supply are in a disconnected state; When the controller receives a signal for horizontally moving the lifting assembly, the controller sends a start signal to the second execution element that controls the opening and closing of the driving motor of the lifting assembly, so that the driving motor and the external power supply are in a conducting state, so that the lifting assembly moves horizontally; when the lifting assembly moves to a specified position, the controller sends a shutdown signal to the second execution element that controls the opening and closing of the driving motor of the lifting assembly, so that the driving motor and the external power supply are in a disconnected state; When the controller receives a signal that causes the hook of the lifting assembly to be raised or lowered, the controller sends a start signal to the third execution element that controls the opening and closing of the lifting motor of the lifting assembly, so that the lifting motor is in a conductive state with the external power supply, so that the hook of the lifting motor rises or falls; when the hook of the lifting motor rises or falls to a specified position, the controller sends a shutdown signal to the third execution element that controls the opening and closing of the lifting motor, so that the lifting or falling of the hook of the lifting motor is disconnected from the external power supply; The controller receives a first liquid level signal detected by the first liquid level sensor (108), and converts the first liquid level signal into a first digital signal; when the first digital signal is less than a preset liquid replenishment starting value, the controller sends a first control signal to the liquid replenishment valve (112) and the liquid inlet valve (113), and the liquid replenishment valve (112) and the liquid inlet valve (113) are opened, so that external liquid is replenished to the bottom of the heat-insulating tank body (101) through the liquid inlet pipe (109); The controller receives a first temperature signal or a second temperature signal corresponding to the first temperature sensor (110) or the second temperature sensor, and converts the first temperature signal or the second temperature signal into a second digital signal or a third digital signal respectively; when one of the second digital signal or the third digital signal is greater than a pre-set exhaust setting value in the corresponding control unit, the controller sends a second control signal to the liquid inlet valve (113) and the exhaust valve (114), and the liquid inlet valve (113) is closed and the exhaust valve (114) is opened; otherwise, the controller sends a third control signal to the liquid inlet valve (113) and the exhaust valve (114), and the liquid replenishing valve (112) and the liquid inlet valve (113) are opened, and the exhaust valve (114) is closed; The controller receives a second liquid level signal detected by the first liquid level sensor (108), and converts the second liquid level signal into a second digital signal. When the second digital signal is greater than a preset liquid replenishment stop value, the controller sends a fourth control signal to the liquid replenishment valve (112), the liquid inlet valve (113), and the exhaust valve (114), and the liquid replenishment valve (112), the liquid inlet valve (113), and the exhaust valve (114) are closed.

9. A multifunctional low-temperature automatic preservation system for biological samples according to claim 8, characterized in that: The storage system also includes a laser locator, which is arranged on one side of the lifting frame.

10. A multifunctional low-temperature automatic preservation system for biological samples according to claim 9, characterized in that: A stepped frame (116) is also provided on one side of the bottom of the heat-insulating tank body (101), and an endoscope (501) is also placed on the top of the heat-insulating tank body (101) located in the transition area.

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