Gene sequencing device and gene sequencing process
By reasonably designing the layout of reagent slot components in the gene sequencing device, the problem of inefficient gene sequencing caused by unreasonable reagent slot arrangement in the prior art is solved, and a more efficient gene sequencing process is achieved.
Patent Information
- Application Number
- CN202311014769.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-08-11
- Publication Date
- 2025-02-18
AI Technical Summary
The reagent tank arrangement of existing gene sequencing devices is unreasonable, resulting in messy chip transfer routes, affecting the transfer speed and biochemical reaction efficiency, and thus reducing the gene sequencing efficiency.
Design a gene sequencing device, the reagent tank assembly of the biochemical reaction mechanism is set in sequence according to the order of biochemical reactions, and the amplification reagent area, the hybridization reagent area and the resection synthetic reagent area are placed close to the transfer mechanism to optimize the reagent tank Layout to facilitate chip transfer.
Through reasonable reagent tank layout, the transfer efficiency of the chip in multiple reaction reagent areas is improved, the transfer time is shortened, and the efficiency of gene sequencing is improved.
Smart Images
Figure CN119464032A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of gene sequencing technology, and in particular to a gene sequencing device and a gene sequencing process. Background Art
[0002] In the gene sequencing process, each round of sequencing includes multiple biochemical reactions. When performing biochemical reactions, each chip needs to be transferred in multiple reagent tanks. After multiple biochemical reactions are completed, the chip needs to be transferred to the optical mechanism for photo detection. In the process of realizing this application, the inventor found that the prior art has at least the following problems: the gene sequencing device in the prior art has an unreasonable number and arrangement of reagent tanks. For example, the order of biochemical reactions and the rationality of the loading and unloading positions when the chip is transferred between multiple mechanisms are not taken into account, resulting in a messy route of the chip during transfer, affecting the transfer speed, and resulting in inconvenience; for example, the reagent tank in the reagent tank assembly is not reasonably designed, resulting in too many reagent tanks and too large a size of the reagent tank assembly, which affects the transfer efficiency of the transfer mechanism and makes the biochemical reaction efficiency low, thereby resulting in too low efficiency of gene sequencing. Summary of the invention
[0003] Based on this, the present application provides a gene sequencing device and a gene sequencing process to solve the problem of low gene sequencing efficiency caused by unreasonable arrangement of reagent tanks in the prior art.
[0004] To achieve the above purpose, the technical solution of the embodiment of the present application is implemented as follows:
[0005] On the one hand, an embodiment of the present application provides a gene sequencing device, including a biochemical reaction mechanism, a loading and unloading mechanism, an optical-mechanical mechanism, and a transfer mechanism;
[0006] The biochemical reaction mechanism is used to perform biochemical reactions on the chip; the loading and unloading mechanism is used to carry the chip; the optical-mechanical mechanism is used to perform imaging detection on the chip; the transfer mechanism is used to transfer the chip between the loading and unloading mechanism, the optical-mechanical mechanism and the biochemical reaction mechanism;
[0007] The biochemical reaction mechanism includes a reagent tank assembly, which includes multiple reagent tanks for holding reagents and carrying chips; the reagent tank assembly includes an amplification reagent area, a hybridization reagent area and an excision synthesis reagent area arranged in sequence along a first direction; the amplification reagent area includes a circulation reagent tank group, the excision synthesis reagent area includes a loading reagent tank group and a unloading reagent tank group, and the excision synthesis reagent area is arranged close to the transfer mechanism.
[0008] In one embodiment, the reagent tank assembly includes a reaction zone and a transition zone along the second direction, the transition zone is arranged between two adjacent reaction zones, each reaction zone is respectively provided with a corresponding chip grabbing mechanism, each chip grabbing mechanism is used to transfer the chip within the range between the corresponding reaction zone and the transition zone adjacent to the reaction zone, and the second direction is arranged perpendicular to the first direction.
[0009] In one embodiment, each of the biochemical reaction mechanism, the loading and unloading mechanism, the optical-mechanical mechanism and the transfer mechanism constitutes a gene sequencing unit, and each of the gene sequencing devices includes a plurality of gene sequencing units.
[0010] In one embodiment, the number of chip grabbing mechanisms in each biochemical reaction mechanism is 2, the number of chips reacting simultaneously is a, the number of reagent tanks in the transition zone is b=a / 2; the number of reagent tanks in the loading reagent tank group and the unloading reagent tank group are both b.
[0011] In one embodiment, the circulating reagent tank group in each of the biochemical reaction mechanisms includes a first circulating reagent tank group and a second circulating reagent tank group, and the first circulating reagent tank group and the second circulating reagent tank group each include b+1 reagent tanks.
[0012] In one embodiment, the number of chips reacting simultaneously is 6, the number of reagent slots in the transition zone is 3, and the number of reagent slots in the loading reagent slot group and the unloading reagent slot group are both 3.
[0013] In one embodiment, the number of chips reacting simultaneously is 8, the number of reagent slots in the transition zone is 4, and the number of reagent slots in the loading reagent slot group and the unloading reagent slot group are both 4.
[0014] In one embodiment, the reagent tanks in the reaction zone and the transition zone are arranged perpendicular to each other.
[0015] On the other hand, an embodiment of the present application provides a gene sequencing process for use in the gene sequencing device as described above, comprising the following steps:
[0016] S1, the transfer mechanism transfers the chip from the loading and unloading mechanism to the biochemical reaction mechanism;
[0017] S2, the chip sequentially performs PCR amplification reaction, primer hybridization reaction and excision synthesis reaction in the biochemical reaction mechanism;
[0018] S3, the transfer mechanism transfers the chip to the optical-mechanical mechanism for imaging detection;
[0019] S4, obtaining the number of reaction rounds in step S2, and determining whether the number of reaction rounds reaches a preset number of reaction rounds, if so, ending; if not, repeating steps S2 and S3.
[0020] In one embodiment, the PCR amplification reaction is carried out in the amplification reagent area, and the PCR amplification reaction includes a cycling process, which is carried out in the cycling reagent tank group; the primer hybridization reaction is carried out in the hybridization reagent area; the excision synthesis reaction is carried out in the excision synthesis reagent area, the loading reagent tank group is used to carry chips to be subjected to biochemical reactions, and the unloading reagent tank group is used to carry chips that have completed a round of biochemical reactions.
[0021] The present application has at least the following beneficial effects: The gene sequencing device provided by the present application includes multiple components and can realize the automated process of gene sequencing. The reagent tank assembly of its biochemical reaction mechanism is sequentially arranged with an amplification reagent area, a hybridization reagent area, and an excision synthesis reagent area according to the order of the biochemical reaction, so that the chip can be easily transferred in multiple reaction reagent areas. At the same time, the excision synthesis reagent area is arranged close to the transfer mechanism. When the chip completes the excision synthesis reaction, it can be quickly transferred by the transfer mechanism to the optical machine mechanism for photo detection. The gene sequencing device of the embodiment of the present application has a reasonable reagent tank layout, which effectively improves the efficiency of gene sequencing. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 Schematic diagram of the structure of a gene sequencing device according to an embodiment of the present application.
[0023] Figure 2 for Figure 1 Schematic diagram of the structure of the biochemical reaction mechanism of the gene sequencing device.
[0024] Figure 3 This is a schematic diagram of the arrangement of the reagent tank assembly according to an embodiment of the present application (along the second direction).
[0025] Figure 4 for Figure 3 Schematic diagram of the arrangement of the reagent tank assembly (along the first direction).
[0026] Figure 5 This is a schematic diagram of the arrangement of the reagent tank assembly according to another embodiment of the present application (along the second direction).
[0027] Figure 6 for Figure 5 Schematic diagram of the arrangement of the reagent tank assembly (along the first direction).
[0028] The meanings of the symbols in the accompanying drawings are as follows:
[0029] 1. Biochemical reaction mechanism; 11. Chip grabbing mechanism; 12. Reagent tank assembly; 13. Reaction zone; 14. Transition zone; 15. Amplification reagent zone; 151. First cycle reagent tank group; 152. Second cycle reagent tank group; 16. Hybridization reagent zone; 17. Excision synthesis reagent zone; 171. Loading reagent tank group; 172. Unloading reagent tank group; 18. Reagent tank; 2. Transfer mechanism; 3. Loading and unloading mechanism; 4. Optical mechanism; 5. Chip. DETAILED DESCRIPTION
[0030] The technical solution of the present application is further elaborated in detail below in conjunction with the accompanying drawings and specific embodiments of the specification.
[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit the implementation of this application. The term "and / or" used herein includes any and all combinations of one or more related listed items.
[0032] In the description of the present application, it should be understood that the terms "center", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating positions or positional relationships, are based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as a limitation on the present application. In the description of the present application, unless otherwise specified, "multiple" means two or more.
[0033] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.
[0034] See also Figure 1 and Figure 2 The gene sequencing device of the embodiment of the present application includes a biochemical reaction mechanism 1, a loading and unloading mechanism 3, an optical-mechanical mechanism 4 and a transfer mechanism 2.
[0035] The biochemical reaction mechanism 1 is used to perform biochemical reaction on the chip 5; the loading and unloading mechanism 3 is used to carry the chip 5; the optical mechanism 4 is used to perform imaging detection on the chip 5; the transfer mechanism 2 is used to transfer the chip 5 between the loading and unloading mechanism 3, the optical mechanism 4 and the biochemical reaction mechanism 1. Specifically, the chip 5 to be tested is placed in the loading and unloading mechanism 3, transferred to the biochemical reaction mechanism 1 by the transfer mechanism 2, and after the biochemical reaction is carried out in the biochemical reaction mechanism 1, it is transferred to the optical mechanism 4 by the transfer mechanism 2 for photographic imaging detection. After the detection is completed, the chip 5 is transferred again by the transfer mechanism 2 to the biochemical reaction mechanism 1 for the next round of biochemical reaction, or after the number of biochemical reaction rounds reaches a preset number of rounds, it is transferred by the transfer mechanism 2 to the loading and unloading mechanism 3. In this embodiment, the transfer mechanism 2 is a six-axis manipulator. In other embodiments, the transfer mechanism 2 can also be a transfer device of other structures.
[0036] In order to further improve the working efficiency of the gene sequencing device, multiple gene sequencing units can be set, and each gene sequencing unit includes a group of biochemical reaction mechanisms 1, loading and unloading mechanisms 3, optical and mechanical mechanisms 4 and transfer mechanisms 2. Each gene sequencing unit completes the entire sequencing process of the corresponding chip 5, or multiple gene sequencing units jointly complete the entire sequencing process of the chip 5. That is, one chip 5 completes the entire gene sequencing process only in the corresponding gene sequencing unit, or one chip 5 completes the entire gene sequencing process in multiple gene sequencing units.
[0037] like Figure 2 As shown, the biochemical reaction mechanism 1 includes a reagent tank assembly 12 and a chip grabbing mechanism 11. The reagent tank assembly 12 includes a plurality of reagent tanks 18. The reagent tanks 18 are used to hold reagents and carry chips 5. The reagent tank assembly 12 includes an amplification reagent area 15, a hybridization reagent area 16 and a cutting synthesis reagent area 17 arranged in sequence along the first direction. The amplification reagent area 15 includes a circulation reagent tank group, and the cutting synthesis reagent area 17 includes a loading reagent tank group 171 and a unloading reagent tank group 172. The cutting synthesis reagent area 17 is arranged near the transfer mechanism 2. The reagent tank assembly 12 includes a reaction area 13 and a transition area 14 along the second direction. The transition area 14 is arranged between two adjacent reaction areas 13. Each reaction area 13 is respectively provided with a chip grabbing mechanism 11. Each chip grabbing mechanism 11 is used to transfer the chip 5 within the range between the corresponding reaction area 13 and the transition area 14 adjacent to the reaction area 13. The second direction is arranged perpendicular to the first direction. The loading reagent tank group 171 and the unloading reagent tank group 172 are respectively arranged close to one side of the transfer mechanism 2. The loading reagent tank group 171 is used to carry the chips 5 to be subjected to biochemical reactions, and the unloading reagent tank group 172 is used to carry the chips 5 that have completed a round of biochemical reactions.
[0038] In some embodiments, the number of chip grabbing mechanisms 11 in each biochemical reaction mechanism 1 is 2, the number of chips 5 reacting at the same time is a, the number of reagent tanks 18 in the transition zone 14 is b=a / 2; the number of reagent tanks 18 in the loading reagent tank group 171 and the unloading reagent tank group 172 is both b. The chip grabbing mechanism 11 can be a four-axis manipulator, etc., and the specific structure is not limited, as long as it can realize the transfer of the chip 5 between the corresponding reaction zone 13 and the transition zone 14.
[0039] For example, Figure 3 and Figure 4 As shown, in one embodiment, the number of chip grabbing mechanisms 11 is 2, and correspondingly, the number of reaction zones 13 is also 2, and a transition zone 14 is provided between the two reaction zones 13. The reagent tank 18 of the transition zone 14 is used to store cleaning or pre-cleaning reagents. The reagent tank 18 of the transition zone 14 is not used for biochemical reactions, but is only used for temporary storage when the chip 5 is transferred from one reaction zone 13 to another reaction zone 13. Because the working range of the chip grabbing mechanism 11 only includes the corresponding reaction zone 13 and the adjacent transition zone 14, but does not include another reaction zone 13 adjacent to the transition zone 14, therefore, when a chip 5 needs to be transferred from one reaction zone 13 to the next reaction zone 13, it needs to be transferred to the transition zone 14 for temporary storage. As shown in the figure, the second direction is the horizontal direction in the figure, and the first direction is the vertical direction in the figure. The reagent tank assembly 12 shown in the figure includes a total of 39 reagent tanks 18, among which the three reagent tanks 18 in the middle are transition zones 14, and the remaining 36 reagent tanks 18 are evenly divided into two parts, located on both sides of the transition zone 14, forming two reaction zones 13, and the number of reagent tanks 18 in each reaction zone 13 is 18, arranged in a matrix, and the reagent tanks 18 in the two reaction zones 13 and the reagent tanks 18 in the transition zone 14 are arranged perpendicular to each other, which is conducive to making the overall layout of the reagent tank assembly 12 more compact. The reagent tank assembly 12 is divided into an amplification reagent zone 15, a hybridization reagent zone 16 and an excision synthesis reagent zone 17 along a first direction (the direction in the figure is from top to bottom), specifically, the top two rows of reagent tanks 18 in the two reaction zones 13 are amplification reagent zones 15, the middle two rows are hybridization reagent zones 16, and the bottom two rows are excision synthesis reagent zones 17. In the gene sequencing reaction process, the gene sequencing process includes PCR amplification reaction, primer hybridization reaction and excision synthesis reaction, and the three reaction processes are performed in sequence. The amplification reagent area 15, the hybridization reagent area 16 and the excision synthesis reagent area 17 are arranged in sequence according to the reaction process sequence, which is convenient for the circulation of the chip 5 and is conducive to improving the efficiency of gene sequencing. At the same time, the excision synthesis reagent area 17 is arranged on the side close to the transfer mechanism 2, which is convenient for the transfer mechanism 2 to transfer the chip 5, thereby improving the efficiency of gene sequencing.
[0040] The circulating reagent tank group in the amplification reagent area 15 includes a first circulating reagent tank group 151 and a second circulating reagent tank group 152, and the first circulating reagent tank group 151 and the second circulating reagent tank group 152 respectively include b+1 reagent tanks 18. The specific positions of the first circulating reagent tank group 151 and the second circulating reagent tank group 152 are not limited, as long as they are located in the amplification reagent area 15, and preferably are located in different reaction areas 13 respectively. For example, in this embodiment, the number of reagent tanks 18 in the first circulating reagent tank group 151 is 4, and the number of reagent tanks 18 in the second circulating reagent tank group 152 is also 4. The first circulating reagent tank group 151 is arranged in the reaction area 13 on the left side of the figure, and the second circulating reagent tank group 152 is arranged in the reaction area 13 on the right side of the figure. The first circulating reagent tank group 151 includes 4 reagent tanks 18, and the second circulating reagent tank group 152 also includes 4 reagent tanks 18, which can respectively meet the PCR amplification reaction of 3 chips 5 at the same time, and a total of 6 chips 5 can be reacted simultaneously. The circulating reagent tank group is used to hold circulating reagents that need to be used multiple times. The provision of the circulating reagent tank 18 can reduce the total number of reagent tanks 18, making the structure of the reagent tank assembly 12 more compact. In order to allow three chips 5 to perform circulating reactions at the same time, the number of reagent tanks 18 in a circulating reagent tank group is at least one more than the number of chips 5. The extra reagent tank 18 is used to temporarily store the chips 5, and the cleaning reagent can be held therein.
[0041] The cut-out synthesis reagent area 17 includes a loading reagent tank group 171 and a unloading reagent tank group 172. The number of reagent tanks 18 in the loading reagent tank group 171 is 3, and the number of unloading reagent tank group 172 is also 3. The loading reagent tank group 171 and the unloading reagent tank group 172 are respectively arranged close to the transfer mechanism 2 to facilitate the transfer of the transfer mechanism 2. They can be respectively arranged in the same reaction area 13, or in different reaction areas 13.
[0042] For example, Figure 5 and Figure 6As shown, in one embodiment, the number of chip grabbing mechanisms 11 is 2, and correspondingly, the number of reaction zones 13 is also 2, and a transition zone 14 is provided between the two reaction zones 13. As shown in the figure, the second direction is the horizontal direction in the figure, and the first direction is the vertical direction in the figure. The reagent tank assembly 12 shown in the figure includes a total of 48 reagent tanks 18, wherein the middle 4 reagent tanks 18 are transition zones 14, and the remaining 44 reagent tanks 18 are evenly divided into two parts, located on both sides of the transition zone 14, forming two reaction zones 13, and the number of reagent tanks 18 in each reaction zone 13 is 22, arranged in a matrix, and the reagent tanks 18 in the two reaction zones 13 and the reagent tanks 18 in the transition zone 14 are arranged perpendicular to each other. The reagent tank assembly 12 is divided into an amplification reagent area 15, a hybridization reagent area 16 and a cutting synthesis reagent area 17 along a first direction (the direction in the figure is from top to bottom). Specifically, the top four rows of reagent tanks 18 in the two reaction areas 13 are amplification reagent areas 15 (the top eight reagent tanks 18 in each reaction area 13 are amplification reagent areas 15, for a total of 16 reagent tanks 18), the middle seven reagent tanks 18 in each reaction area 13 are hybridization reagent areas 16 (for a total of 14 reagent tanks 18), and the bottom seven reagent tanks 18 in each reaction area 13 are cutting synthesis reagent areas 17 (for a total of 14 reagent tanks 18).
[0043] In this embodiment, the number of reagent slots 18 in the first circulating reagent slot group 151 is 5, and the number of reagent slots 18 in the second circulating reagent slot group 152 is also 5. The first circulating reagent slot group 151 is arranged in the reaction area 13 on the left side of the diagram, and the second circulating reagent slot group 152 is arranged in the reaction area 13 on the right side of the diagram. The first circulating reagent slot group 151 includes 5 reagent slots 18, and the second circulating reagent slot group 152 also includes 5 reagent slots 18, which can respectively meet the PCR amplification reaction of 4 chips 5 at the same time, and a total of 8 chips 5 can be reacted at the same time.
[0044] The cut-out synthesis reagent area 17 includes a loading reagent tank group 171 and a unloading reagent tank group 172. The number of reagent tanks 18 in the loading reagent tank group 171 is 4, and the number of unloading reagent tank group 172 is also 4. The loading reagent tank group 171 and the unloading reagent tank group 172 are respectively arranged close to the transfer mechanism 2 to facilitate the transfer of the transfer mechanism 2. They can be respectively arranged in the same reaction area 13, or they can be respectively arranged in different reaction areas 13.
[0045] The present application embodiment also provides a gene sequencing process, which is used in the above-mentioned gene sequencing device, and includes the following steps:
[0046] S1 . The transfer mechanism 2 transfers the chip 5 from the loading and unloading mechanism 3 to the biochemical reaction mechanism 1 .
[0047] The chip 5 to be reacted is placed in the loading and unloading mechanism 3, and is fed to the loading port of the loading and unloading mechanism 3 through the loading and unloading mechanism 3. The transfer mechanism 2 grabs the chip 5 at the loading port and places it in the loading reagent slot group 171 of the cut-out synthesis reagent area 17, and loading is performed from this position.
[0048] S2. The chip 5 performs PCR amplification reaction, primer hybridization reaction and excision synthesis reaction in sequence in the biochemical reaction mechanism 1.
[0049] The PCR amplification reaction is carried out in the amplification reagent area 15. The PCR amplification reaction includes a circulation process, and the circulation process is carried out in the circulation reagent tank group. The primer hybridization reaction is carried out in the hybridization reagent area 16. The excision synthesis reaction is carried out in the excision synthesis reagent area 17. The reagents contained in the reagent tanks 18 in the three reagent areas do not overlap except for the cleaning reagents. In order to ensure that the dripping does not affect the biochemical reaction when the chip 5 is transferred, the cleaning reagent can be inserted between the main reagents to clean the chip 5.
[0050] The chip grabbing mechanism 11 corresponding to the loading reagent slot group 171 sequentially grabs the chip 5 in the loading reagent slot group 171 to the amplification reagent area 15 for PCR amplification reaction; after the PCR amplification reaction is completed, the chip grabbing mechanism 11 sequentially grabs the chip 5 to the hybridization reagent area 16 for primer hybridization reaction; after the primer hybridization reaction is completed, the chip grabbing mechanism 11 sequentially grabs the chip 5 to the excision synthesis reagent area 17 for excision synthesis reaction.
[0051] S3. The transfer mechanism 2 transfers the chip 5 to the optical-mechanical mechanism 4 for imaging detection.
[0052] After the excision synthesis reaction is completed, the chip grabbing mechanism 11 sequentially grabs the chips 5 to the optical mechanism 4 for photographic imaging detection.
[0053] S4, obtaining the number of reaction rounds in step S2, and determining whether the number of reaction rounds reaches a preset number of reaction rounds. If so, the process ends; if not, steps S2 and S3 are repeated.
[0054] After the imaging detection is completed, the specific reaction round of step S2 is obtained. When the number of reaction rounds reaches the preset number of reaction rounds, the biochemical reaction ends, and the transfer mechanism 2 transfers the chip 5 to the loading and unloading mechanism 3; when the number of reaction rounds does not reach the preset number of reaction rounds, steps S2 and S3 are repeated until the preset number of reaction rounds is reached and the reaction ends.
[0055] The gene sequencing device of the embodiment of the present application has a reasonable layout and a compact structure, and can increase the speed of the gene sequencing process, thereby improving the efficiency of gene sequencing.
[0056] It should be noted that, in this article, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "comprises a ..." does not exclude the existence of other identical elements in the process, method, article or device including the element.
[0057] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.
Claims
1. A gene sequencing device, characterized in that: Including biochemical reaction mechanism, loading and unloading mechanism, optical-mechanical mechanism and transfer mechanism; The biochemical reaction mechanism is used to perform biochemical reactions on the chip; the loading and unloading mechanism is used to carry the chip; the optical-mechanical mechanism is used to perform imaging detection on the chip; the transfer mechanism is used to transfer the chip between the loading and unloading mechanism, the optical-mechanical mechanism and the biochemical reaction mechanism; The biochemical reaction mechanism includes a reagent tank assembly, which includes multiple reagent tanks for holding reagents and carrying chips; the reagent tank assembly includes an amplification reagent area, a hybridization reagent area and an excision synthesis reagent area arranged in sequence along a first direction; the amplification reagent area includes a circulation reagent tank group, the excision synthesis reagent area includes a loading reagent tank group and a unloading reagent tank group, and the excision synthesis reagent area is arranged close to the transfer mechanism.
2. The gene sequencing device according to claim 1, characterized in that: The reagent tank assembly includes a reaction zone and a transition zone along the second direction, the transition zone is arranged between two adjacent reaction zones, each reaction zone is respectively provided with a corresponding chip grabbing mechanism, each chip grabbing mechanism is used to transfer the chip within the range between the corresponding reaction zone and the transition zone adjacent to the reaction zone, and the second direction is arranged perpendicular to the first direction.
3. The gene sequencing device according to claim 2, wherein: Each of the biochemical reaction mechanisms, the loading and unloading mechanisms, the optical-mechanical mechanisms and the transfer mechanisms constitute a gene sequencing unit, and each of the gene sequencing devices includes a plurality of gene sequencing units.
4. The gene sequencing device according to claim 3, characterized in that: The number of chip grabbing mechanisms in each biochemical reaction mechanism is 2, the number of chips reacting simultaneously is a, the number of reagent tanks in the transition zone is b=a / 2; the number of reagent tanks in the loading reagent tank group and the unloading reagent tank group is both b.
5. The gene sequencing device according to claim 4, characterized in that: The circulating reagent tank group in each of the biochemical reaction mechanisms includes a first circulating reagent tank group and a second circulating reagent tank group, and the first circulating reagent tank group and the second circulating reagent tank group each include b+1 reagent tanks.
6. The gene sequencing device according to claim 5, characterized in that: The number of chips reacting simultaneously is 6, the number of reagent slots in the transition zone is 3, and the number of reagent slots in the loading reagent slot group and the unloading reagent slot group are both 3.
7. The gene sequencing device according to claim 5, characterized in that: The number of chips reacting simultaneously is 8, the number of reagent slots in the transition zone is 4, and the number of reagent slots in the loading reagent slot group and the unloading reagent slot group are both 4.
8. The gene sequencing device according to claim 2, wherein: The reagent tanks in the reaction zone and the transition zone are arranged perpendicular to each other.
9. A gene sequencing process, characterized in that: A gene sequencing device as claimed in any one of claims 1 to 8, comprising the following steps: S1, the transfer mechanism transfers the chip from the loading and unloading mechanism to the biochemical reaction mechanism; S2, the chip sequentially performs PCR amplification reaction, primer hybridization reaction and excision synthesis reaction in the biochemical reaction mechanism; S3, the transfer mechanism transfers the chip to the optical-mechanical mechanism for imaging detection; S4, obtaining the number of reaction rounds in step S2, and determining whether the number of reaction rounds reaches a preset number of reaction rounds, if so, ending; if not, repeating steps S2 and S3.
10. The gene sequencing process according to claim 9, characterized in that: The PCR amplification reaction is carried out in the amplification reagent area, and the PCR amplification reaction includes a circulation process, which is carried out in the circulation reagent tank group; the primer hybridization reaction is carried out in the hybridization reagent area; the excision synthesis reaction is carried out in the excision synthesis reagent area, the loading reagent tank group is used to carry the chip to be subjected to biochemical reaction, and the unloading reagent tank group is used to carry the chip that has completed a round of biochemical reaction.