Molecular detection method and device based on optical fiber probe and multi-channel optical fiber sensing system

Abstract

The invention provides a molecule detection method and device based on an optical fiber probe and a multi-channel optical fiber sensing system. The method comprises the following steps: acquiring a sampling signal; determining a target binding rate according to the sampling signal; and determining the target concentration of the target to-be-detected molecule in the to-be-detected sample according to the target binding rate and the rate mapping relation set. According to the embodiment of the invention, the concentration of the target to-be-detected molecule in the to-be-detected sample is obtained by analyzing the target binding rate of the target to-be-detected molecule and the target target on the sensing unit within the preset time period, so that on one hand, the time for waiting for the sensing unit to reach a stable target target adsorption state is saved, and the detection efficiency is improved; the influence of the arrangement state of the target object at the binding site on detection in the process that the sensing unit adsorbs the target object can be reduced, and the detection accuracy can be improved.

Description

Technical Field

[0001] The present application belongs to the field of optical fiber biological detection technology, and specifically relates to a molecular detection method and device based on optical fiber probes and a multi-channel optical fiber sensing system. Background Art

[0002] At present, SPR (Surface plasmon resonance) sensing technology has become a widely used optical detection method. The detection method is to fully contact the SPR sensor with the sample to be tested, and the target target modified on the sensing unit of the SPR sensor specifically binds to the molecule to be tested in the sample to be tested. Then, the spectral signal of the molecule to be tested in a steady state is sampled by the sensing unit of the SPR sensor, and the spectral signal is analyzed to obtain the detection result. However, in the actual detection process, the time consumed by the sensing unit of the SPR sensor to bind to the molecule to be tested to reach a steady state is too long, often requiring tens of minutes, and the longer the consumption time, the greater the influence of the arrangement state of the molecule to be tested on the binding site of the sensing unit on the detection accuracy, which is specifically manifested in that the sensing unit of the SPR sensor has a large influence on the detection accuracy. The unit has multiple binding sites for the molecules to be tested. The molecules to be tested bind to the target targets on the binding sites, and the arrangement state of the molecules to be tested is not always vertical, and there are often partial tilts or complete tilts. The molecules to be tested with abnormal arrangement states will occupy more than two binding sites, and other molecules to be tested can no longer bind to these binding sites. When the molecules to be tested and the target targets initially bind, there are sufficient binding sites, and the molecules to be tested can easily find binding sites. The arrangement state of the molecules to be tested has little effect on the detection accuracy, but as time goes on, the abnormal arrangement state of the molecules to be tested leads to an increasing number of abnormally reduced binding sites, and even an extreme situation occurs where there are a large number of remaining molecules to be tested but no binding sites can be found, and the accuracy of the detection results is affected. Summary of the invention

[0003] The embodiments of the present application provide a molecular detection method, device and multi-channel optical fiber sensing system based on an optical fiber probe, which obtains the concentration of the target molecule to be detected in the sample to be detected by analyzing the target binding rate between the target molecule to be detected and the target target on the sensing unit within a preset time period. Compared with analyzing the corresponding sampling signal under the state of stable adsorption of the target target on the sensing unit to obtain the concentration of the target molecule to be detected in the sample to be detected, on the one hand, it saves the time of waiting for the sensing unit to reach the state of stable adsorption of the target target, which is beneficial to improving the detection efficiency. On the other hand, it can reduce the influence of the arrangement state of the target target at the binding site on the detection during the process of the sensing unit adsorbing the target target, which is beneficial to improving the accuracy of the detection.

[0004] In a first aspect, an embodiment of the present application provides a molecular detection method based on an optical fiber probe, which is applied to a processing device in an optical fiber sensing system, wherein the optical fiber sensing system includes a light source, an incident optical fiber, an optical fiber sensor, an output optical fiber, a processing device, and a mixing device, wherein the mixing device includes a mixing chamber, wherein the incident optical fiber is respectively connected to the light source and the optical fiber sensor, wherein the output optical fiber is respectively connected to the optical fiber sensor and the processing device, wherein the mixing chamber includes a reaction residual sample, wherein the reaction residual sample is a sample remaining after the sample to be tested reacts with a fixed amount of a target target, wherein the optical fiber sensor includes a sensing unit, wherein the sensing unit is modified with a target molecule to be tested that specifically binds to the target target; wherein the method includes: Acquiring a sampling signal from the optical fiber sensor; Determine the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal; The target concentration of the target molecule to be detected in the sample to be detected is determined according to the target binding rate and a rate mapping relationship set, wherein the rate mapping relationship set includes a correspondence between the binding rate and the concentration of the target molecule to be detected in the sample to be detected.

[0005] In a second aspect, an embodiment of the present application provides a multi-channel fiber optic sensing system, the multi-channel fiber optic sensing system comprising a light source, a first detection channel, a second detection channel, a channel switch and a processing device, the first detection channel comprising an incident optical fiber, a first optical fiber sensing unit and an exiting optical fiber, the second detection channel comprising a fourth incident optical fiber, a second optical fiber sensing unit and a fourth exiting optical fiber, the incident optical fiber being respectively connected to the first optical fiber sensing unit and the light source, the fourth incident optical fiber being respectively connected to the second optical fiber sensing unit and the light source, the exiting optical fiber being respectively connected to the first optical fiber sensing unit and the channel switch, the fourth exiting optical fiber being respectively connected to the second optical fiber sensing unit and the channel switch, the channel switch being connected to the processing device, the first optical fiber sensing unit comprising the optical fiber sensor as described in the first aspect, and the second optical fiber sensing unit comprising the optical fiber sensor as described in the first aspect.

[0006] In a third aspect, an embodiment of the present application provides a molecular detection device based on an optical fiber probe, which is applied to a processing device in an optical fiber sensing system, wherein the optical fiber sensing system includes a light source, an incident optical fiber, an optical fiber sensor, an output optical fiber, a processing device and a mixing device, wherein the mixing device includes a mixing chamber, wherein the incident optical fiber is respectively connected to the light source and the optical fiber sensor, wherein the output optical fiber is respectively connected to the optical fiber sensor and the processing device, wherein the mixing chamber includes a reaction residual sample, wherein the reaction residual sample is a sample remaining after the sample to be tested reacts with a fixed amount of a target target, wherein the optical fiber sensor includes a sensing unit, wherein the sensing unit is modified with a target molecule to be tested that specifically binds to the target target, and wherein the molecular detection device based on the optical fiber probe includes an acquisition unit and a determination unit, wherein: The acquisition unit is used to acquire a sampling signal from the optical fiber sensor; The determination unit is used to determine the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal; The determination unit is further used to determine the target concentration of the target molecule to be detected in the sample to be detected according to the target binding rate and a rate mapping relationship set, wherein the rate mapping relationship set includes a correspondence between the binding rate and the concentration of the target molecule to be detected in the sample to be detected.

[0007] In a fourth aspect, an embodiment of the present application provides a computer-readable storage medium having a computer program / instructions stored thereon, which, when executed by a processor, implement the steps in the first aspect of the embodiment of the present application.

[0008] In a fifth aspect, an embodiment of the present application provides a computer program product, wherein the computer program product includes a non-transitory computer-readable storage medium storing a computer program, and the computer program is operable to enable a computer to execute part or all of the steps described in the first aspect of the embodiment of the present application.

[0009] It can be seen that in the embodiment of the present application, the processing device first obtains the sampling signal from the optical fiber sensor, and then determines the target binding rate of the target molecule to be detected on the sensing unit and the target target in the residual sample of the reaction within a preset time period according to the sampling signal, and then determines the target concentration of the target molecule to be detected in the sample to be detected according to the target binding rate and the rate mapping relationship set, and the rate mapping relationship set includes the corresponding relationship between the binding rate and the concentration of the target molecule to be detected in the sample to be detected. By analyzing the target binding rate of the target molecule to be detected on the sensing unit and the target target in the preset time period, the concentration of the target molecule to be detected in the sample to be detected is obtained. Compared with analyzing the corresponding sampling signal under the state of stable adsorption of the target target on the sensing unit to obtain the concentration of the target molecule to be detected in the sample to be detected, on the one hand, it saves the time waiting for the sensing unit to reach the state of stable adsorption of the target target, which is conducive to improving the detection efficiency. On the other hand, it can reduce the influence of the arrangement state of the target target at the binding site on the detection during the process of the sensing unit adsorbing the target target, which is conducive to improving the accuracy of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0011] Figure 1 is a schematic diagram of the architecture of an optical fiber sensing system provided in an embodiment of the present application; Figure 2 It is a schematic diagram of a molecular detection method based on an optical fiber probe provided in an embodiment of the present application; Figure 3A An optical fiber sensor with a reflective portion provided in an embodiment of the present application; Figure 3B It is a U-shaped optical fiber sensor provided in an embodiment of the present application; Figure 3C It is another optical fiber sensor with a reflective portion provided in an embodiment of the present application; Figure 3D It is another U-shaped optical fiber sensor provided in an embodiment of the present application; Figure 3E A schematic diagram of a spectral signal is shown; Figure 3F is a schematic diagram of a change in a characterization value provided in an embodiment of the present application; Figure 4 is a schematic diagram of a multi-channel optical fiber sensing system provided in an embodiment of the present application; Figure 5It is a structural schematic diagram of an electronic device provided in an embodiment of the present application; Figure 6 This is a block diagram of the functional units of a molecular detection device based on an optical fiber probe provided in an embodiment of the present application. DETAILED DESCRIPTION

[0012] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0013] The terms "first", "second", etc. in the specification and claims of this application and the above-mentioned drawings are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.

[0014] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0015] In the embodiments of the present application, "and / or" describes the association relationship of the associated objects, indicating that three relationships may exist. For example, A and / or B can represent the following three situations: A exists alone; A and B exist at the same time; B exists alone. Among them, A and B can be singular or plural.

[0016] In the embodiment of the present application, the symbol " / " can indicate that the objects associated with each other are in an "or" relationship. In addition, the symbol " / " can also indicate a division sign, that is, performing a division operation. For example, A / B can indicate A divided by B.

[0017] In the embodiments of the present application, "at least one item" or similar expressions refer to any combination of these items, including any combination of single items or plural items, and refer to one or more, and multiple refers to two or more. For example, at least one item of a, b, or c can represent the following seven situations: a, b, c, a and b, a and c, b and c, a, b, and c. Among them, each of a, b, and c can be an element or a set containing one or more elements.

[0018] In the embodiments of the present application, "equal to" can be used in conjunction with greater than, and is applicable to the technical solution adopted when greater than, and can also be used in conjunction with less than, and is applicable to the technical solution adopted when less than. When equal to is used in conjunction with greater than, it is not used in conjunction with less than; when equal to is used in conjunction with less than, it is not used in conjunction with greater than.

[0019] In order to better understand the solutions of the embodiments of the present application, the electronic devices, related concepts and backgrounds that may be involved in the embodiments of the present application are first introduced below.

[0020] The electronic devices involved in the embodiments of the present application may include various handheld devices with wireless communication functions, vehicle-mounted devices, wearable devices, computing devices or other processing devices connected to a wireless modem, as well as various forms of user equipment (UE), mobile stations (MS), electronic devices (terminal devices), etc. For the convenience of description, the above-mentioned devices are collectively referred to as electronic devices. The electronic device can also be a processing device in an optical fiber biological detection system.

[0021] See also Figure 1 , Figure 1 Schematic diagram of the architecture of an optical fiber sensing system provided in an embodiment of the present application. Figure 1 As shown, the fiber optic sensing system 1 includes a light source 10 , an incident optical fiber 20 , a fiber optic sensor 30 , an output optical fiber 40 , a processing device 50 and a mixing device 60 .

[0022] Among them, the incident optical fiber 20 is respectively connected to the light source 10 and the optical fiber sensor 30, the output optical fiber 40 is respectively connected to the optical fiber sensor 30 and the processing device 50, the mixing device 60 includes a mixing chamber, the mixing chamber includes a reaction residual sample, and the reaction residual sample is a sample left after the sample to be tested reacts with a fixed amount of target target.

[0023] The optical fiber sensor 30 includes a sensing unit, on which a target molecule to be detected that specifically binds to the target is modified, and the optical fiber sensor 30 detects the concentration of the target molecule to be detected in the sample to be detected by an indirect competition method. The sensing unit includes a single-mode optical fiber and a thin film deposited on the surface of the single-mode optical fiber, and the thin film can be a metal film that can efficiently generate surface plasma or other sensing materials that can excite plasma resonance reaction, such as gold; The mixing device 60 may be a constant temperature mixer, and the mixing device 60 is used to mix the sample to be tested and the target object in its cavity at a preset temperature.

[0024] The light source 10 may be monochromatic light or broadband white light, which is not limited here.

[0025] The optical fiber sensor 30 may be a single optical fiber sensor or a dual optical fiber sensor, which is not limited here.

[0026] The processing device 50 may be a spectrometer, the processing device 50 may also include a photoelectric conversion chip and a processor, and the processing device 50 may also include an optical path switch and a spectrometer.

[0027] The input optical fiber 20 and the output optical fiber 40 may be multimode optical fibers.

[0028] The optical fiber sensing system 1 may include an optical fiber holder, and the optical fiber holder is used to fix the optical fiber sensor 30 so as to be inserted into the mixing cavity with the optical fiber sensor 30 for detection.

[0029] In one possible example, the processing device 50 first obtains a sampling signal from the optical fiber sensor 30, and then determines the target binding rate of the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset period of time according to the sampling signal, and then determines the target concentration of the target molecule to be detected in the sample to be detected according to the target binding rate and the rate mapping relationship set, and the rate mapping relationship set includes the corresponding relationship between the binding rate and the concentration of the target molecule to be detected in the sample to be detected. By analyzing the target binding rate of the target molecule to be detected on the sensing unit and the target target in the preset period of time, the concentration of the target molecule to be detected in the sample to be detected is obtained. Compared with analyzing the corresponding sampling signal under the state of stable adsorption of the target target on the sensing unit to obtain the concentration of the target molecule to be detected in the sample to be detected, on the one hand, the time of waiting for the sensing unit to reach the state of stable adsorption of the target target is saved, which is conducive to improving the detection efficiency, and on the other hand, it can reduce the influence of the arrangement state of the target target at the binding site on the detection during the process of the sensing unit adsorbing the target target, which is conducive to improving the accuracy of the detection.

[0030] See also Figure 2 , Figure 2The present invention is a flow chart of a molecular detection method based on an optical fiber probe provided in an embodiment of the present application, which is applied to a processing device in an optical fiber sensing system, wherein the optical fiber sensing system comprises a light source, an incident optical fiber, an optical fiber sensor, an output optical fiber, a processing device and a mixing device, wherein the mixing device comprises a mixing chamber, wherein the incident optical fiber is respectively connected to the light source and the optical fiber sensor, wherein the output optical fiber is respectively connected to the optical fiber sensor and the processing device, wherein the mixing chamber comprises a reaction residual sample, wherein the reaction residual sample is a sample remaining after the sample to be tested reacts with a fixed amount of a target target, wherein the optical fiber sensor comprises a sensing unit, wherein the sensing unit is modified with a target molecule to be tested that specifically binds to the target target; wherein the method comprises: Step S201, obtaining a sampling signal from the optical fiber sensor.

[0031] When the optical fiber probe, ie, the optical fiber sensor, is inserted into the reaction residual sample in the mixing cavity, the processing device can collect the sampling signal of the outgoing light from the optical fiber sensor in real time.

[0032] The sensing unit first modifies the target molecule to be detected and then blocks it with a blocking agent, which may be bovine serum albumin.

[0033] The sample to be tested can be serum. Since Hcy molecules are easily bound to proteins in the blood and are unstable in nature, in order to detect the content of Hcy molecules in serum, the Hcy protein complex in the sample to be tested can be reduced and then hydrolyzed into S-(5-adenosine)-L-homocysteine ​​(SAH), which is the target molecule to be tested. The target targeting substance can be the antibody Anti-SAH of SAH. The sample to be tested can be added to the mixing chamber first. After the sample to be tested is reduced and hydrolyzed, a fixed amount of the target targeting substance Anti-SAH is added. After the reaction is complete, a residual sample of the reaction is obtained. The optical fiber sensor is inserted into the residual sample of the reaction. The SAH modified on the optical fiber sensor specifically binds to the Anti-SAH in the residual sample of the reaction. The SAH modified on the optical fiber sensor forms an indirect competitive relationship with the reduced and hydrolyzed SAH of the sample to be tested.

[0034] The amount of the target substance can be set manually or by system default, and is not limited here.

[0035] Among them, the optical fiber sensor can be a single optical fiber sensor or a dual optical fiber sensor.

[0036] Optionally, see Figure 3A , Figure 3AAn optical fiber sensor with a reflective part is provided in an embodiment of the present application. The sensing unit includes a first sensing part. The optical fiber sensor includes the first transmission part, the first sensing part and the first reflective part. The first transmission part is respectively connected to the incident optical fiber and the first sensing part, and the first sensing part is connected to the first reflective part. The light transmission path is as follows (arrow direction): the incident light of the light source enters the first transmission part through the incident optical fiber, then enters the first sensing part, then enters the first reflective part, enters the first sensing part after being reflected by the first reflective part, then enters the first transmission part, and passes through the outgoing optical fiber to the processing device.

[0037] Optionally, see Figure 3B , Figure 3B A U-shaped optical fiber sensor provided by an embodiment of the present application, wherein the sensing unit comprises a second sensing part, and the optical fiber sensor comprises a second transmission part, a second sensing part, and a third transmission part; the third transmission part comprises a first bending part and a first body part, one end of the first bending part is bent and connected to the second sensing part, one end of the first body part is bent and connected to the other end of the first bending part, the first body part and the third transmission part are arranged oppositely, and the first body part and the second sensing part are arranged oppositely, the second transmission part is connected to the incident optical fiber, and the third transmission part is connected to the output optical fiber. The transmission direction of the incident light is consistent with the direction of the arrow in the figure.

[0038] Optionally, see Figure 3C , Figure 3C It is another optical fiber sensor with a reflector provided in an embodiment of the present application. The optical fiber sensing system includes a splitter coupler, the optical fiber sensor includes a first sensor and a second sensor, the output optical fiber includes a first output optical fiber and a second output optical fiber, and the input optical fiber includes a first input optical fiber and a second input optical fiber; the splitter coupler includes a first port, a second port, a third port and a fourth port, the first sensor includes a fourth transmission part, a third sensing part and a second reflector, and the second sensor includes a fifth transmission part, a fourth sensing part and a third reflector; the third sensing part is respectively connected to the fourth transmission part and the second reflector, the fourth sensing part is respectively connected to the fifth transmission part and the third reflector, the fourth transmission part is connected to the first input optical fiber through the first port, the fifth transmission part is connected to the second input optical fiber through the second port, the third port is connected to the processing device through the first output optical fiber, and the fourth port is connected to the processing device through the second output optical fiber. The processing device includes an optical path switcher and a spectrometer, the first output optical fiber and the second output optical fiber are respectively connected to the optical path switcher, and the optical path switcher is connected to the spectrometer.

[0039] The third sensing part is directly blocked with a blocking agent, and the fourth sensing part is first modified with a target molecule to be detected and then blocked with a blocking agent.

[0040] Among them, a third sampling signal from the first sensor at the first time point can be obtained, and a fourth sampling signal from the second sensor at the first time point can be obtained; a first characterization value can be determined based on the third sampling signal and the fourth sampling signal, a fifth sampling signal from the first sensor at the second time point can be obtained, and a sixth sampling signal from the second sensor at the second time point can be obtained; a second characterization value is determined based on the fifth sampling signal and the sixth sampling signal, and a target characterization value is determined based on the first characterization value and the second characterization value.

[0041] For example, the light source is broadband white light, the first characterization value = the fourth resonance peak wavelength of the fourth sampling signal - the third resonance peak wavelength of the third sampling signal, or the light source is monochromatic light, the first characterization value = the fourth light intensity value of the fourth sampling signal - the third light intensity value of the third sampling signal.

[0042] Among them, the third sampling signal is a reference signal of the fourth sampling signal, which is used to eliminate the influence of conditions such as temperature, background liquid concentration, and component conditions at the first time point; the fifth sampling signal is a reference signal of the sixth sampling signal, which is used to eliminate the influence of conditions such as temperature, background liquid concentration, and component conditions at the second time point.

[0043] Optionally, see Figure 3D , Figure 3D It is another U-shaped optical fiber sensor provided in an embodiment of the present application, and the optical fiber sensor includes a sixth transmission part, a fifth sensing part, a seventh transmission part, a sixth sensing part and an eighth transmission part, the two ends of the seventh transmission part are respectively connected to one end of the fifth sensing part and one end of the sixth sensing part, the other end of the fifth sensing part is connected to one end of the sixth transmission part, the other end of the sixth sensing part is connected to one end of the eighth transmission part, the other end of the sixth transmission part is connected to the incident optical fiber, the other end of the eighth transmission part is connected to the output optical fiber, the seventh transmission part is bent, the fifth sensing part and the sixth sensing part are symmetrically arranged, and the sixth transmission part and the eighth transmission part are symmetrically arranged.

[0044] Among them, the fifth sensing part is directly blocked with a blocking agent, and the sixth sensing part is first modified with a target molecule to be detected and then blocked with a blocking agent.

[0045] Wherein, the light source is broadband white light; determining the first characterization value according to the first sampling signal, and determining the second characterization value according to the second sampling signal include the following steps: determining the fifth resonance peak wavelength and the sixth resonance peak wavelength in the first sampling signal, determining the seventh resonance peak wavelength and the eighth resonance peak wavelength in the second sampling signal, determining the first characterization value according to the fifth resonance peak wavelength and the sixth resonance peak wavelength, determining the second characterization value according to the seventh resonance peak wavelength and the eighth resonance peak wavelength, and determining the target characterization value according to the first characterization value and the second characterization value.

[0046] Among them, the first characterization value=|fifth resonance peak wavelength-sixth resonance peak wavelength|, and the second characterization value=|seventh resonance peak wavelength-eighth resonance peak wavelength|.

[0047] Step S202 , determining a target binding rate between the target molecule to be detected on the sensor unit and the target target in the remaining reaction sample within a preset time period according to the sampling signal.

[0048] Among them, the higher the content of the target molecule to be tested in the sample to be tested, the smaller the amount of the fixed amount of target targeting substance remaining after the mixed reaction with the target molecule to be tested in the sample to be tested, then the light signal detected by the optical fiber sensor changes weaker, that is, the target binding rate between the target molecule to be tested on the sensing unit and the target targeting substance in the remaining sample in the reaction within the preset time period is smaller, the lower the content of the target molecule to be tested in the sample to be tested, the more the fixed amount of target targeting substance remaining after the mixed reaction with the target molecule to be tested in the sample to be tested, then the light signal detected by the optical fiber sensor changes stronger, and the target binding rate between the target molecule to be tested on the sensing unit and the target targeting substance in the remaining sample in the reaction within the preset time period is greater.

[0049] Among them, the preset time period can be set manually or by system default, and is not limited here. For example, the preset time period is 10 seconds, which means that a 10-second quick test of the target molecule in the sample to be tested can be achieved. Compared with traditional detection methods that take dozens of minutes, this greatly saves the time cost of detection.

[0050] Step S203, determining the target concentration of the target molecule to be detected in the sample to be detected according to the target binding rate and the rate mapping relationship set, wherein the rate mapping relationship set includes the corresponding relationship between the binding rate and the concentration of the target molecule to be detected in the sample to be detected.

[0051] Among them, the binding rate in the rate mapping relationship set is negatively correlated with the concentration of the target molecule to be tested in the sample to be tested, and the corresponding relationship between the binding rate in the rate mapping relationship set and the concentration of the target molecule to be tested in the sample to be tested can be determined in advance through a large number of experiments. The binding rate is positively correlated with the logarithmic concentration of the target molecule to be tested in the sample to be tested.

[0052] Among them, the target binding rate can be used to query the rate mapping relationship set to obtain the target concentration corresponding to the target binding rate. When the target molecule to be detected is SAH, after obtaining the target concentration of SAH, the concentration of Hcy can be further obtained based on the conversion relationship between SAH and Hcy.

[0053] It can be seen that in this example, the processing device first obtains the sampling signal from the optical fiber sensor, and then determines the target binding rate of the target molecule to be measured on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal, and then determines the target concentration of the target molecule to be measured in the sample to be measured according to the target binding rate and the rate mapping relationship set, and the rate mapping relationship set includes the corresponding relationship between the binding rate and the concentration of the target molecule to be measured in the sample to be measured. By analyzing the target binding rate of the target molecule to be measured on the sensing unit and the target target in the preset time period, the concentration of the target molecule to be measured in the sample to be measured is obtained. Compared with analyzing the corresponding sampling signal under the state of stable adsorption of the target target on the sensing unit to obtain the concentration of the target molecule to be measured in the sample to be measured, on the one hand, it saves the time waiting for the sensing unit to reach a stable adsorption state of the target target, which is conducive to improving the detection efficiency. On the other hand, it can reduce the influence of the arrangement state of the target target at the binding site on the detection during the process of the sensing unit adsorbing the target target, which is conducive to improving the accuracy of the detection.

[0054] In one possible example, in terms of obtaining a sampling signal from the optical fiber sensor, the method may include the following steps: obtaining a first sampling signal from the optical fiber sensor at a first time point, the first time point being the time point at which the target molecule to be measured on the sensing unit begins to react with the target target; and obtaining a second sampling signal from the optical fiber sensor at a second time point, the second time point being the time point after the preset time period has passed from the first time point.

[0055] Among them, the processing equipment can sample the sampling signal of the output light of the optical fiber sensor in real time, and analyze the two adjacent sampling signals by windowing to determine whether the signal change occurs in the latter sampling signal compared with the previous sampling signal. Specifically, when the light source is monochromatic light, it is analyzed whether the light intensity value corresponding to the monochromatic light wavelength in the latter sampling signal changes compared with the light intensity value corresponding to the monochromatic light wavelength in the previous sampling signal. If it increases or decreases, the time point corresponding to the latter sampling signal is used as the first time point, the latter sampling signal is used as the first sampling signal, and the sampling signal at the second time point after the preset time period of the first time point is used as the second sampling signal; when the light source is broadband white light, it is analyzed whether the resonance peak wavelength in the latter sampling signal changes compared with the resonance peak wavelength in the previous sampling signal. If it increases or decreases, the latter sampling signal is used as the first sampling signal. The time point corresponding to the sampling signal is taken as the first time point, the next sampling signal is taken as the first sampling signal, and the sampling signal at the second time point after the preset time period of the first time point is taken as the second sampling signal. Optionally, when the light source is broadband white light, the target axis is determined, the first closed figure formed by the target axis and the first spectrum graph corresponding to the next sampling signal is determined, and the second closed figure formed by the second spectrum graph corresponding to the previous sampling signal of the target axis is determined, the first wavelength of the first centroid of the first closed figure is determined, and the second wavelength of the second centroid of the second closed figure is determined. When it is determined that the first wavelength is not equal to the second wavelength, the time point corresponding to the next sampling signal is taken as the first time point, the next sampling signal is taken as the first sampling signal, and the sampling signal at the second time point after the preset time period of the first time point is taken as the second sampling signal. Please refer to Figure 3E , Figure 3E A schematic diagram of a spectral signal is shown, where the horizontal axis is the light wavelength in nm, the vertical axis is the output light intensity, the target axis and the waveform graph have two intersection points, the closed area enclosed by the target axis and the waveform graph is a closed figure, the target axis is parallel to the horizontal axis, and the vertical distance between the target axis and the horizontal axis is in the range of [0.9-0.98].

[0056] The target binding rate is the average binding rate within a preset period of time when the target molecule to be detected on the sensor unit begins to react with the target target.

[0057] It can be seen that in this example, by selecting the first sampling signal sampled at the first time point and the second sampling signal sampled at the second time point, and analyzing the first sampling signal and the second sampling signal to obtain the target binding rate, and further determining the target concentration based on the target binding rate, it is beneficial to improve the accuracy of detection, and it is beneficial to improve the detection efficiency.

[0058] In one possible example, in terms of determining the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period based on the sampling signal, the above method may include the following steps: determining a first characterization value based on the first sampling signal, and determining a second characterization value based on the second sampling signal, the first characterization value being used to characterize the absorption capacity of the sensing unit for the first incident light of the light source at the first time point, and the second characterization value being used to characterize the absorption capacity of the sensing unit for the second incident light of the light source at the second time point; determining a target change value based on the first characterization value and the second characterization value; and determining the target binding rate based on the target change value and the preset time period.

[0059] Among them, when the film surface medium of the sensing unit changes, the refractive index of the film surface medium also changes. Correspondingly, the sensing unit's ability to absorb incident light also changes. Specifically, when the target molecules to be measured modified on the film surface begin to gather target targets, the refractive index of the film surface medium increases.

[0060] The target variation characterization value is used to characterize the variation of the absorption capacity of the optical fiber sensor to the incident light within the interval between the first time point and the second time point.

[0061] Wherein, target binding rate = target change value / preset time period.

[0062] Among them, see Figure 3F , Figure 3F This is a schematic diagram of a change in a characterization value provided in an embodiment of the present application. The horizontal axis is time and the vertical axis is the characterization value. The figure shows a first time point and a second time point. The characterization value corresponding to the first time point is the first characterization value, and the characterization value corresponding to the second time point is the second characterization value.

[0063] It can be seen that in this example, the target binding rate is obtained by analyzing the first sampling signal and the second sampling signal, and the target concentration is further determined according to the target binding rate, which is beneficial to improving the accuracy of detection and the detection efficiency.

[0064] In one possible example, the light source is broadband white light; in terms of determining the first characterization value according to the first sampling signal, and determining the second characterization value according to the second sampling signal, the following steps may be included: determining a first resonance peak wavelength in the first sampling signal, and using the first resonance peak wavelength as the first characterization value; determining a second resonance peak wavelength in the second sampling signal, and using the second resonance peak wavelength as the second characterization value.

[0065] The first resonance peak wavelength is a wavelength value corresponding to a peak point in a waveform diagram corresponding to the first sampling signal, and the second resonance peak wavelength is a wavelength value corresponding to a peak point in a waveform diagram corresponding to the second sampling signal.

[0066] Optionally, the waveform graph corresponding to the target axis and the first sampling signal forms a third closed figure, the waveform graph corresponding to the target axis and the second sampling signal forms a fourth closed figure, the wavelength value corresponding to the centroid of the third closed figure is the first characterization value, and the wavelength value corresponding to the centroid of the fourth closed figure is the second characterization value.

[0067] It can be seen that in this example, the target binding rate is obtained by analyzing the first sampling signal and the second sampling signal, and the target concentration is further determined according to the target binding rate, which is beneficial to improving the accuracy of detection and the detection efficiency.

[0068] In one possible example, the light source is monochromatic light; in terms of determining the first characterization value based on the first sampling signal, and determining the second characterization value based on the second sampling signal, the following steps may be included: determining a first light intensity value corresponding to the wavelength of the monochromatic light in the first sampling signal, and using the first light intensity value as the first characterization value; determining a second light intensity value corresponding to the wavelength of the monochromatic light in the second sampling signal, and using the second light intensity value as the second characterization value.

[0069] The wavelength of the monochromatic light belongs to the first wavelength segment or the second wavelength segment. The wavelength range of the first wavelength segment includes 500nm to 580nm; the wavelength range of the second wavelength segment includes 660nm to 780nm.

[0070] It can be seen that in this example, selecting monochromatic light in the first wavelength range or the second wavelength range for detection is beneficial to improving detection efficiency compared to locating the resonance peak wavelength.

[0071] See also Figure 4 , Figure 4 It is a schematic diagram of a multi-channel fiber optic sensing system provided in an embodiment of the present application, wherein the multi-channel fiber optic sensing system includes a light source, multiple detection channels, a channel switch and a processing device, wherein a single detection channel includes an incident optical fiber, a fiber optic sensing unit, an output optical fiber and a mixing device, wherein the incident optical fiber is respectively connected to the fiber optic sensing unit and the light source, the output optical fiber is respectively connected to the fiber optic sensing unit and the channel switch, the channel switch is connected to the processing device, and the fiber optic sensing unit includes the aforementioned fiber optic sensor.

[0072] The multiple detection channels include a first detection channel, a second detection channel, ..., an nth detection channel.

[0073] The optical fiber sensing units of different detection channels can be used to detect the same or different molecules to be detected in parallel under the same conditions or different conditions, which is not limited here.

[0074] See also Figure 5 , Figure 5 : is a structural schematic diagram of an electronic device provided in an embodiment of the present application, which is applied to a processing device in an optical fiber sensing system, wherein the optical fiber sensing system comprises a light source, an incident optical fiber, an optical fiber sensor, an output optical fiber, a processing device and a mixing device, wherein the mixing device comprises a mixing chamber, wherein the incident optical fiber is respectively connected to the light source and the optical fiber sensor, wherein the output optical fiber is respectively connected to the optical fiber sensor and the processing device, wherein the mixing chamber comprises a reaction residual sample, wherein the reaction residual sample is a sample remaining after the sample to be tested reacts with a fixed amount of a target target, wherein the optical fiber sensor comprises a sensing unit, wherein the sensing unit is modified with a target molecule to be tested that specifically binds to the target target; Figure 5 As shown, the electronic device includes a processor, a memory, a communication interface, and one or more programs, wherein the one or more programs are stored in the memory, and the one or more programs are configured to execute the following steps by the processor: Acquiring a sampling signal from the optical fiber sensor; Determine the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal; The target concentration of the target molecule to be detected in the sample to be detected is determined according to the target binding rate and a rate mapping relationship set, wherein the rate mapping relationship set includes a correspondence between the binding rate and the concentration of the target molecule to be detected in the sample to be detected.

[0075] It can be seen that in the embodiment of the present application, the electronic device first obtains the sampling signal from the optical fiber sensor, and then determines the target binding rate of the target molecule to be tested on the sensing unit and the target target in the residual sample of the reaction within a preset time period according to the sampling signal, and then determines the target concentration of the target molecule to be tested in the sample to be tested according to the target binding rate and the rate mapping relationship set, and the rate mapping relationship set includes the corresponding relationship between the binding rate and the concentration of the target molecule to be tested in the sample to be tested. The concentration of the target molecule to be tested in the sample to be tested is obtained by analyzing the target binding rate of the target molecule to be tested on the sensing unit and the target target in the preset time period. Compared with analyzing the corresponding sampling signal under the state of stable adsorption of the target target on the sensing unit to obtain the concentration of the target molecule to be tested in the sample to be tested, on the one hand, it saves the time waiting for the sensing unit to reach the state of stable adsorption of the target target, which is conducive to improving the detection efficiency. On the other hand, it can reduce the influence of the arrangement state of the target target at the binding site on the detection during the process of the sensing unit adsorbing the target target, which is conducive to improving the accuracy of the detection.

[0076] In a possible example, in terms of acquiring a sampling signal from the optical fiber sensor, the program includes instructions for executing the following steps: Acquire a first sampling signal from the optical fiber sensor at a first time point, wherein the first time point is a time point when the target molecule to be detected on the sensing unit begins to react with the target target; A second sampling signal is obtained from the optical fiber sensor at a second time point, where the second time point is a time point after the first time point has passed the preset period of time.

[0077] In one possible example, in determining the target binding rate between the target molecule to be detected on the sensor unit and the target in the remaining sample of the reaction within a preset time period according to the sampling signal, the program includes instructions for executing the following steps: Determine a first characterization value according to the first sampling signal, and determine a second characterization value according to the second sampling signal, wherein the first characterization value is used to characterize the absorption capacity of the sensing unit to the first incident light of the light source at the first time point, and the second characterization value is used to characterize the absorption capacity of the sensing unit to the second incident light of the light source at the second time point; Determining a target variation value according to the first characterization value and the second characterization value; The target binding rate is determined according to the target change value and the preset time period.

[0078] In a possible example, the light source is broadband white light; in determining the first characterization value according to the first sampling signal, and determining the second characterization value according to the second sampling signal, the program includes instructions for performing the following steps: Determine a first resonance peak wavelength in the first sampling signal, and use the first resonance peak wavelength as the first characterization value; According to the second resonance peak wavelength in the second sampling signal, the second resonance peak wavelength is used as the second characterization value.

[0079] In a possible example, the light source is monochromatic light; in terms of determining the first characterization value according to the first sampling signal, and determining the second characterization value according to the second sampling signal, the program further includes instructions for performing the following steps: Determine a first light intensity value corresponding to the wavelength of the monochromatic light in the first sampling signal, and use the first light intensity value as the first characterization value; A second light intensity value corresponding to the wavelength of the monochromatic light in the second sampling signal is determined, and the second light intensity value is used as the second characterization value.

[0080] The above mainly introduces the scheme of the embodiment of the present application from the perspective of the execution process on the method side. It is understandable that, in order to realize the above functions, the electronic device includes a hardware structure and / or software module corresponding to the execution of each function. Those skilled in the art should easily realize that, in combination with the units and algorithm steps of each example described in the embodiment provided in this article, the present application can be implemented in the form of hardware or a combination of hardware and computer software. Whether a function is executed in the form of hardware or computer software driving hardware depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this application.

[0081] The embodiment of the present application can divide the electronic device into functional units according to the above method example. For example, each functional unit can be divided according to each function, or two or more functions can be integrated into one processing unit. The above integrated unit can be implemented in the form of hardware or in the form of software functional units. It should be noted that the division of units in the embodiment of the present application is schematic and is only a logical function division. There may be other division methods in actual implementation.

[0082] In the case of dividing each functional module into corresponding functional modules, Figure 6 is a block diagram of the functional units of a molecular detection device based on an optical fiber probe provided in an embodiment of the present application, such as Figure 6As shown, a processing device used in an optical fiber sensing system, the optical fiber sensing system includes a light source, an incident optical fiber, an optical fiber sensor, an output optical fiber, a processing device and a mixing device, the mixing device includes a mixing chamber, the incident optical fiber is respectively connected to the light source and the optical fiber sensor, the output optical fiber is respectively connected to the optical fiber sensor and the processing device, the mixing chamber includes a reaction residual sample, the reaction residual sample is a sample left after the sample to be tested reacts with a fixed amount of target target, the optical fiber sensor includes a sensing unit, the sensing unit is modified with a target molecule to be tested that specifically binds to the target target; the molecular detection device based on the optical fiber probe includes: an acquisition unit 601 and a determination unit 602, wherein, The acquisition unit 601 is used to acquire a sampling signal from the optical fiber sensor; The determination unit 602 is used to determine the target binding rate between the target molecule to be detected on the sensor unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal; The determination unit 602 is further configured to determine a target concentration of the target molecule to be detected in the sample to be detected according to the target binding rate and a rate mapping relationship set, wherein the rate mapping relationship set includes a correspondence between binding rate and concentration of the target molecule to be detected in the sample to be detected.

[0083] It can be seen that the optical fiber biological detection device described in the embodiment of the present application can first obtain a sampling signal from the optical fiber sensor, and then determine the target binding rate between the target molecule to be detected on the sensing unit and the target target in the residual sample of the reaction within a preset time period according to the sampling signal, and then determine the target concentration of the target molecule to be detected in the sample to be detected according to the target binding rate and the rate mapping relationship set, and the rate mapping relationship set includes the corresponding relationship between the binding rate and the concentration of the target molecule to be detected in the sample to be detected. By analyzing the target binding rate between the target molecule to be detected and the target target on the sensing unit within a preset time period, the concentration of the target molecule to be detected in the sample to be detected is obtained. Compared with analyzing the corresponding sampling signal under the state of stable adsorption of the target target on the sensing unit to obtain the concentration of the target molecule to be detected in the sample to be detected, on the one hand, it saves the time of waiting for the sensing unit to reach a stable adsorption state of the target target, which is conducive to improving the detection efficiency. On the other hand, it can reduce the influence of the arrangement state of the target target at the binding site on the detection during the process of the sensing unit adsorbing the target target, which is conducive to improving the accuracy of the detection.

[0084] In a possible example, in terms of acquiring the sampling signal from the optical fiber sensor, the acquiring unit 601 is specifically configured to: Acquire a first sampling signal from the optical fiber sensor at a first time point, wherein the first time point is a time point when the target molecule to be detected on the sensing unit begins to react with the target target; A second sampling signal is obtained from the optical fiber sensor at a second time point, where the second time point is a time point after the first time point has passed the preset period of time.

[0085] In a possible example, in determining the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal, the determining unit 602 is specifically used to: Determine a first characterization value according to the first sampling signal, and determine a second characterization value according to the second sampling signal, wherein the first characterization value is used to characterize the absorption capacity of the sensing unit to the first incident light of the light source at the first time point, and the second characterization value is used to characterize the absorption capacity of the sensing unit to the second incident light of the light source at the second time point; Determining a target variation value according to the first characterization value and the second characterization value; The target binding rate is determined according to the target change value and the preset time period.

[0086] In a possible example, the light source is broadband white light; in determining the first characterization value according to the first sampling signal, and determining the second characterization value according to the second sampling signal, the determining unit 602 is specifically configured to: Determine a first resonance peak wavelength in the first sampling signal, and use the first resonance peak wavelength as the first characterization value; According to the second resonance peak wavelength in the second sampling signal, the second resonance peak wavelength is used as the second characterization value.

[0087] In a possible example, the light source is monochromatic light; in determining the first characterization value according to the first sampling signal, and determining the second characterization value according to the second sampling signal, the determining unit 602 is specifically configured to: Determine a first light intensity value corresponding to the wavelength of the monochromatic light in the first sampling signal, and use the first light intensity value as the first characterization value; A second light intensity value corresponding to the wavelength of the monochromatic light in the second sampling signal is determined, and the second light intensity value is used as the second characterization value.

[0088] It should be noted that all relevant contents of each step involved in the above method embodiment can be referred to the functional description of the corresponding functional module and will not be repeated here.

[0089] The electronic device provided in this embodiment is used to execute the above-mentioned molecular detection method based on optical fiber probe, and thus can achieve the same effect as the above-mentioned implementation method.

[0090] In the case of an integrated unit, the electronic device may include a processing module, a storage module and a communication module. The processing module may be used to control and manage the actions of the electronic device, for example, it may be used to support the electronic device to execute the steps performed by the above-mentioned functional unit. The storage module may be used to support the electronic device to execute stored program codes and data, etc. The communication module may be used to support the communication between the electronic device and other devices.

[0091] Among them, the processing module can be a processor or a controller. It can implement or execute various exemplary logic boxes, modules and circuits described in conjunction with the disclosure of this application. The processor can also be a combination that implements computing functions, such as a combination of one or more microprocessors, a combination of digital signal processing (DSP) and a microprocessor, etc. The storage module can be a memory. The communication module can specifically be a device that interacts with other electronic devices, such as a radio frequency circuit, a Bluetooth chip, a Wi-Fi chip, etc.

[0092] An embodiment of the present application also provides a computer storage medium, wherein the computer storage medium stores a computer program for electronic data exchange, wherein the computer program enables a computer to execute part or all of the steps of any method described in the above method embodiments, and the above computer includes an electronic device.

[0093] The present application also provides a computer program product, which includes a non-transitory computer-readable storage medium storing a computer program, and the computer program is operable to cause a computer to execute some or all of the steps of any method described in the method embodiment. The computer program product may be a software installation package, and the computer includes a control platform.

[0094] It should be noted that, for the aforementioned method embodiments, for the sake of simplicity, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present application is not limited by the described order of actions, because according to the present application, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily required by the present application.

[0095] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0096] In the several embodiments provided in the present application, it should be understood that the disclosed device can be implemented in other ways. For example, the device embodiments described above are only schematic, such as the division of the above-mentioned units, which is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, and the indirect coupling or communication connection of devices or units can be electrical or other forms.

[0097] The units described above as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0098] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of software functional units.

[0099] If the above-mentioned integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable memory. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a memory, including a number of instructions to enable a computer device (which can be a personal computer, server or network device, etc.) to execute all or part of the steps of the above-mentioned methods in each embodiment of the present application. The aforementioned memory includes: U disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), mobile hard disk, disk or optical disk and other media that can store program codes.

[0100] A person of ordinary skill in the art can understand that all or part of the steps in the various methods of the above embodiments can be completed by instructing related hardware through a program, and the program can be stored in a computer-readable memory, which can include: a flash drive, a read-only memory, a random access memory, a magnetic disk or an optical disk, etc.

[0101] The embodiments of the present application are introduced in detail above. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and core idea of ​​the present application. At the same time, for general technical personnel in this field, according to the idea of ​​the present application, there will be changes in the specific implementation method and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A molecular detection method based on optical fiber probe, characterized in that: A processing device used in an optical fiber sensing system, the optical fiber sensing system comprising a light source, an incident optical fiber, an optical fiber sensor, an output optical fiber, a processing device and a mixing device, the mixing device comprising a mixing chamber, the incident optical fiber being respectively connected to the light source and the optical fiber sensor, the output optical fiber being respectively connected to the optical fiber sensor and the processing device, the mixing chamber comprising a reaction residual sample, the reaction residual sample being a sample remaining after a sample to be tested reacts with a fixed amount of a target target, the optical fiber sensor comprising a sensing unit, the sensing unit being modified with a target molecule to be tested that specifically binds to the target target; the method comprising: Acquiring a sampling signal from the optical fiber sensor; Determine the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal; The target concentration of the target molecule to be detected in the sample to be detected is determined according to the target binding rate and a rate mapping relationship set, wherein the rate mapping relationship set includes a correspondence between the binding rate and the concentration of the target molecule to be detected in the sample to be detected.

2. The method according to claim 1, characterized in that The sensing unit comprises a first sensing part, and the optical fiber sensor comprises a first transmission part, the first sensing part and a first reflection part; The first transmission part is connected to the incident optical fiber and the first sensing part respectively, and the first sensing part is connected to the first reflecting part.

3. The method according to claim 1, characterized in that The sensing unit includes a second sensing part, and the optical fiber sensor includes a second transmission part, the second sensing part and a third transmission part; The third transmission part includes a first bending part and a first main body part, one end of the first bending part is bent and connected to the second sensing part, one end of the first main body part is bent and connected to the other end of the first bending part, the first main body part and the third transmission part are arranged opposite to each other, and the first main body part and the second sensing part are arranged opposite to each other, the second transmission part is connected to the incident optical fiber, and the third transmission part is connected to the output optical fiber.

4. The method according to claim 2 or 3, characterized in that: The acquiring of a sampling signal from the optical fiber sensor comprises: Acquire a first sampling signal from the optical fiber sensor at a first time point, wherein the first time point is a time point when the target molecule to be detected on the sensing unit begins to react with the target target; A second sampling signal is obtained from the optical fiber sensor at a second time point, where the second time point is a time point after the first time point has passed the preset period of time.

5. The method according to claim 4, characterized in that The step of determining the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal comprises: Determine a first characterization value according to the first sampling signal, and determine a second characterization value according to the second sampling signal, wherein the first characterization value is used to characterize the absorption capacity of the sensing unit to the first incident light of the light source at the first time point, and the second characterization value is used to characterize the absorption capacity of the sensing unit to the second incident light of the light source at the second time point; Determining a target variation value according to the first characterization value and the second characterization value; The target binding rate is determined according to the target change value and the preset time period.

6. The method according to claim 5, characterized in that The light source is broadband white light; determining a first characterization value according to the first sampling signal, and determining a second characterization value according to the second sampling signal, comprises: Determine a first resonance peak wavelength in the first sampling signal, and use the first resonance peak wavelength as the first characterization value; According to the second resonance peak wavelength in the second sampling signal, the second resonance peak wavelength is used as the second characterization value.

7. The method according to claim 5, characterized in that The light source is monochromatic light; determining a first characterization value according to the first sampling signal, and determining a second characterization value according to the second sampling signal, comprises: Determine a first light intensity value corresponding to the wavelength of the monochromatic light in the first sampling signal, and use the first light intensity value as the first characterization value; A second light intensity value corresponding to the wavelength of the monochromatic light in the second sampling signal is determined, and the second light intensity value is used as the second characterization value.

8. The method according to claim 1, characterized in that The binding rate in the rate mapping relationship set is negatively correlated with the concentration of the target molecule to be detected in the sample to be detected.

9. A multi-channel optical fiber sensing system, characterized in that: The multi-channel fiber optic sensing system includes a light source, multiple detection channels, a channel switch and a processing device. A single detection channel includes an incident optical fiber, a fiber optic sensing unit, an output optical fiber and a mixing device. The incident optical fiber is respectively connected to the fiber optic sensing unit and the light source, the output optical fiber is respectively connected to the fiber optic sensing unit and the channel switch, the channel switch is connected to the processing device, and the fiber optic sensing unit includes the fiber optic sensor as described in any one of claims 1 to 8.

10. A molecular detection device based on an optical fiber probe, characterized in that: A processing device used in an optical fiber sensing system, the optical fiber sensing system comprising a light source, an incident optical fiber, an optical fiber sensor, an output optical fiber, a processing device and a mixing device, the mixing device comprising a mixing chamber, the incident optical fiber is respectively connected to the light source and the optical fiber sensor, the output optical fiber is respectively connected to the optical fiber sensor and the processing device, the mixing chamber comprises a reaction residual sample, the reaction residual sample is a sample left after the sample to be tested reacts with a fixed amount of target target, the optical fiber sensor comprises a sensing unit, the sensing unit is modified with a target molecule to be tested that specifically binds to the target target, the molecular detection device based on the optical fiber probe comprises an acquisition unit and a determination unit, wherein, The acquisition unit is used to acquire a sampling signal from the optical fiber sensor; The determination unit is used to determine the target binding rate between the target molecule to be detected on the sensing unit and the target target in the remaining sample of the reaction within a preset time period according to the sampling signal; The determination unit is further used to determine the target concentration of the target molecule to be detected in the sample to be detected according to the target binding rate and a rate mapping relationship set, wherein the rate mapping relationship set includes a correspondence between the binding rate and the concentration of the target molecule to be detected in the sample to be detected.

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