Automated molecular operating system

Abstract

The invention provides an automatic molecular operation system, which is used for performing nucleic acid extraction, nucleic acid amplification, primer labeling or reverse transcription on a centrifuge tube for accommodating a specimen, and mainly comprises at least one centrifuge tube bearing module, a transportation module, a plurality of temperature control modules, a capping module, a magnetic field module and an automatic processing module, the automatic processing module is electrically connected with the transportation module, the plurality of temperature control modules, the capping module and the magnetic field module, and controls the transportation module to move the centrifugal tube bearing module, so that the centrifugal tubes accommodated in the centrifugal tube bearing module react in the plurality of temperature control modules; the magnetic field module or the capping unit can be provided for the centrifugal tube as required, so that nucleic acid extraction, nucleic acid amplification, primer labeling, reverse transcription or a combination thereof can be automatically performed on a specimen, errors caused by manual operation are reduced, and the convenience of operation is improved.

Description

Technical Field

[0001] The present invention relates to a nucleic acid detection device, and in particular to an automated molecular operating system that can automatically cycle liquid specimens such as blood, saliva, urine and cerebrospinal fluid at different reaction temperatures for several times according to the required reaction temperature to achieve the required biological operation steps such as nucleic acid extraction, nucleic acid amplification, primer labeling or reverse transcription. Background Art

[0002] With the development of modern medical technology and clinical needs, many diagnostic methods or judgments gradually require quantitative analysis of target genes, especially the use of polymerase chain reaction (PCR) technology to increase the number of nucleic acid detection targets in a short period of time, so that a small amount of nucleic acid detection targets in the specimen can be increased to a detectable amount, which is conducive to the subsequent detection of the presence of the nucleic acid detection target to be detected, and even the determination of its quantity; therefore, the polymerase chain reaction technology has been widely used in medical and biological experimental detection.

[0003] However, during the polymerase chain reaction, multiple cycles of different temperatures are required, and each cycle of different temperatures can increase the number of nucleic acid detection targets in the sample. Therefore, when the sample undergoes a polymerase chain reaction, each cycle generally involves first increasing the temperature to denature the DNA template of the sample, then cooling the temperature to perform primer annealing, and then increasing the temperature again to perform DNA synthesis in the sample, thereby increasing the number of nucleic acids of the target in the sample, and after multiple cycles, sufficient nucleic acids can be obtained for detection. However, most of the above procedures are performed manually, and therefore, the sample needs to be taken or moved by personnel during the heating, cooling and other procedures, which may cause inconsistent sample temperature changes or increase the time for manual waiting for the sample to react. Furthermore, when the user performs nucleic acid extraction, nucleic acid amplification or primer labeling on the sample in a single-function device, since the sample needs to be transferred between devices with different functions, not only may the sample be damaged due to poor temperature control, but also unnecessary errors may occur during manual operation. In view of this, the inventor has devoted himself to research and combined what he has learned to address the above problems and propose a method that can automatically extract nucleic acid, amplify nucleic acid, label primers or a combination thereof according to set procedures and requirements in the same system environment to overcome the deficiencies of the prior art. Summary of the invention

[0004] The object of the present invention is to provide an automated molecular operating system for performing nucleic acid extraction, nucleic acid amplification, primer labeling, reverse transcription or a combination thereof on nucleic acids from liquid specimens such as blood, saliva, urine and cerebrospinal fluid in an automated manner in a single system environment.

[0005] To achieve the above-mentioned purpose, the present invention provides an automated molecular operating system for performing biological operations such as nucleic acid extraction, nucleic acid amplification, primer labeling or reverse transcription on a centrifuge tube containing a specimen, wherein the specimen is one of liquid specimens such as blood, saliva, urine or cerebrospinal fluid, and the automated molecular operating system mainly comprises at least one centrifuge tube carrying module, a transport module, a plurality of temperature control modules, a capping module, a magnetic field module and an automated processing module, and each of the centrifuge tube carrying modules is provided with a plurality of first accommodating holes, to at least one first snap hole and at least one second snap hole, wherein the plurality of first receiving holes are arranged between the first snap holes and the second snap holes; the transport module has a first slide rail unit and a clamping drive unit, and the clamping drive unit is assembled on the first slide rail unit, and the clamping drive unit clamps a centrifuge tube carrying module and moves along the axial direction of the first slide rail unit; the plurality of temperature control modules are each provided with a plurality of second receiving holes and a temperature control unit, wherein the temperature control unit enables the plurality of temperature control modules to reach the desired the required reaction temperature and temperature change mode; the capping module is assembled at one end of the first slide rail unit and is provided with a cover body; the magnetic field module is assembled on the periphery of one of the multiple temperature control modules; and the automatic processing module is electrically connected to the transport module, the multiple temperature control modules, the capping module and the magnetic field module, wherein the automatic processing module controls the temperature control units of the multiple temperature control modules to reach the set reaction temperature or temperature change mode respectively, and controls the clamping drive unit of the transport module to clamp one of the centrifuge tube carrying modules, so that the centrifuge tube carrying module moves along the first slide rail unit, and the centrifuge tube carrying module is selectively moved to the temperature control module with the magnetic field module for nucleic acid extraction, or is placed in the multiple temperature control modules with different temperatures in turn for nucleic acid amplification, or is placed in one of the temperature control modules for filling the reagent containing primers for primer labeling; and when the clamping drive unit places one of the centrifuge tube carrying modules in one of the temperature control modules, the multiple first accommodating holes are arranged relative to the multiple second accommodating holes.

[0006] Furthermore, the clamping drive unit is provided with a first body, a first driver, a second body, a second slide rail unit, a first support arm, a second support arm, a first clamp, a second clamp and a second driver, and the first body is assembled at one end of the first driver and is located on the first slide rail unit, and the first driver is arranged on the periphery of the first slide rail unit, and the first body is assembled with the second body above it, and the second body is provided with the second slide rail unit on a side, and the first support arm and the second support arm are respectively slidably arranged on the second On opposite sides of the slide rail unit, one end of the first arm is assembled with the first clamp and the other end is assembled with one end of the second driver, and one end of the second arm is assembled with the second clamp and the other end is assembled with the other end of the second driver, and the second driver is assembled on the second body, wherein the first driver controls the axial movement of the second body along the first driver, and enables the first driver to control a first distance between the second body and the first body, and the second driver controls a second distance between the first clamp and the second clamp.

[0007] Furthermore, the second driver is provided with a rotating drive block, a first connecting rod and a second connecting rod, one end of the first connecting rod is connected to the first support arm for axial movement along the second slide rail unit, and the other end is pivotally connected to one end of the rotating drive block, and one end of the second connecting rod is connected to the second support arm for axial movement along the second slide rail unit, and the other end is pivotally connected to the other end of the rotating drive block.

[0008] Furthermore, the other end of the first clamp relatively assembled on the first support arm is respectively provided with a first locking groove, and the other end of the second clamp relatively assembled on the second support arm is respectively provided with a second locking groove, wherein after the clamping drive unit clamps a centrifuge tube carrying module, the rotating drive block of the second driver shortens the second distance, and the first locking groove is limited and locked to the first locking hole of the clamped centrifuge tube carrying module, and the second locking groove is limited and locked to the first locking hole and the second locking hole of the clamped centrifuge tube carrying module.

[0009] Furthermore, the covering module is provided with a third driver and at least one auxiliary rod. The third driver is arranged on the peripheral side of the first slide rail unit, and one end of the third driver is assembled with the cover body. The auxiliary rod is adjacent to the third driver and one end is respectively assembled with the cover body.

[0010] Furthermore, the magnetic field module is provided with a fourth driver, at least one telescopic rod and a magnetic field generator. The fourth driver is assembled on the periphery of the first slide rail unit, and one end of the telescopic rod is assembled on the fourth driver, and the other end is assembled with the magnetic field generator. The fourth driver controls the length of the telescopic rod to adjust the distance between the magnetic field generator and the temperature control module.

[0011] Further, the automated processing module controls the clamping drive unit to clamp a centrifuge tube carrying module and move it first to the temperature control module with the magnetic field module for nucleic acid extraction, and then add a reagent containing a primer to one of the temperature control modules for primer labeling, or clamp a centrifuge tube carrying module and move it first to the temperature control module with the magnetic field module for nucleic acid extraction, and then clamp the centrifuge tube carrying module and place it in turn in the multiple temperature control modules with different temperatures for nucleic acid amplification, or clamp a centrifuge tube carrying module and move it first to the temperature control module with the magnetic field module for nucleic acid extraction, and then clamp the centrifuge tube carrying module and place it in turn in the multiple temperature control modules with different temperatures for nucleic acid amplification, and then add a reagent containing a primer to one of the temperature control modules for primer labeling.

[0012] Therefore, the automated molecular manipulation system of the present invention has the following beneficial effects compared to the prior art:

[0013] An automated molecular operating system of the present invention is mainly controlled by the automated processing module to control the clamping drive unit of the transport module, so that the clamping drive unit clamps a centrifuge tube carrying module and enables it to move axially along the first slide rail unit according to a set mode, so that the specimen in the centrifuge tube placed in the multiple first accommodating holes of the centrifuge tube carrying module can be automatically moved to the temperature control module with the magnetic field module for nucleic acid extraction, or placed in the multiple temperature control modules with different temperatures in turn for nucleic acid amplification, or placed in one of the temperature control modules to fill the reagent containing primers for primer labeling, or nucleic acid is carried out before the temperature control module with the magnetic field module. After extraction, it is selected according to needs whether to place the sample in the plurality of temperature-controlled modules with different temperatures in turn for nucleic acid amplification, or whether to place the sample in one of the temperature-controlled modules to add a reagent containing a primer for primer labeling after nucleic acid amplification is completed. In this way, the user can perform nucleic acid extraction, nucleic acid amplification or primer labeling in an automated manner in the automated molecular operating system according to needs, or perform nucleic acid amplification, primer labeling or a combination of the above according to needs after nucleic acid extraction. In this way, the sample can be transferred and reacted in an automated manner in the same system environment, which not only reduces labor costs, but also avoids and reduces errors caused by manual operations and increases the convenience of operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 : is a three-dimensional schematic diagram of the automated molecular operating system of the present invention;

[0015] Figure 2 : is a three-dimensional schematic diagram of the automated molecular operating system of the present invention from another viewing angle;

[0016] Figure 3 : is a three-dimensional exploded schematic diagram of the automated molecular operating system of the present invention;

[0017] Figure 4-1 to Figure 4-2 : It is a schematic diagram of the action of the rotary drive block of the automated molecular operating system of the present invention controlling the clamping drive unit to clamp the centrifuge tube carrying module;

[0018] Figure 4-3 to Figure 4-5 : is a schematic diagram of the action of the clamping drive unit of the automated molecular operating system of the present invention moving the centrifuge tube carrying module to the temperature control module;

[0019] Figure 5 : is a schematic diagram of the action blocks of the automated molecular operating system of the present invention.

[0020]

Explanation of symbols

[0021] 100: Automated Molecular Operating System

[0022] 10: Centrifuge tube carrying module

[0023] 11: First receiving hole

[0024] 12: First buckle hole

[0025] 13: Second buckle hole

[0026] 20: Transport module

[0027] 21: First rail unit

[0028] 22: Clamping drive unit

[0029] 221: First ontology

[0030] 222: First Drive

[0031] 223: Second Body

[0032] 224: Second rail unit

[0033] 225: First arm

[0034] 226: Second arm

[0035] 227: First fixture

[0036] 2271: First stop groove

[0037] 228: Second fixture

[0038] 2281: Second locking groove

[0039] 229: Second Drive

[0040] 2291: Rotary drive block

[0041] 2292: First connecting rod

[0042] 2293: Second connecting rod

[0043] 30: Temperature control module

[0044] 31: Second receiving hole

[0045] 32: Temperature control unit

[0046] 40: Covering module

[0047] 41: Cover

[0048] 42: Third Drive

[0049] 43: Auxiliary rod

[0050] 50: Magnetic field module

[0051] 51: Fourth Drive

[0052] 52: Telescopic rod

[0053] 53: Magnetic field generator

[0054] 60: Automated processing module

[0055] 200: Centrifuge tube

[0056] D1: First distance

[0057] D2: Second distance DETAILED DESCRIPTION

[0058] Hereinafter, several preferred embodiments of the technical features and operation methods of this application are given, and the accompanying drawings are described for reference. Furthermore, the drawings in this invention are not necessarily drawn according to the actual proportions for the convenience of explanation, and the proportions in the drawings are not intended to limit the scope of the protection claimed by this invention.

[0059] For the content of the present invention, please refer to Figures 1 to 5 The figure shows an automated molecular operation system 100 provided by the present invention, which is used to perform biological operations such as nucleic acid extraction, nucleic acid amplification, primer labeling or reverse transcription on a centrifuge tube 200 containing a sample, and the automated molecular operation system 100 includes a plurality of centrifuge tube carrying modules 10, a transport module 20, a plurality of temperature control modules 30, a capping module 40, a magnetic field module 50 and an automated processing module 60. Therefore, please refer to Figure 5As shown, the specimen in the centrifuge tube 200 is one of the liquid specimens including blood, saliva, urine or cerebrospinal fluid. The specimen can be set to perform biological operations such as nucleic acid extraction, nucleic acid amplification, primer labeling or reverse transcription on the specimen according to its needs. The automated molecular operation system 100 then selects corresponding modules and processes from the transport module 20, the temperature control module 30, the capping module 40 and the magnetic field module 50 according to the selected mode, wherein:

[0060] The plurality of centrifuge tube supporting modules 10, each of which is provided with a plurality of first accommodating holes 11, two first buckle holes 12 and two second buckle holes 13, please refer to Figures 1 to 4-5 As shown, the plurality of first receiving holes 11 are disposed between the first buckle hole 12 and the second buckle hole 13;

[0061] The transport module 20 has a first slide rail unit 21 and a clamping drive unit 22, and the clamping drive unit 22 is assembled on the first slide rail unit 21, and the clamping drive unit 22 clamps a centrifuge tube carrying module 10 and moves along the axial direction of the first slide rail unit 21, and the clamping drive unit 22 is further provided with a first body 221, a first driver 222, a second body 223, a second slide rail unit 224, a first arm 225, a second arm 226, a first clamp 227, a second clamp 228 and a second driver 229, and the first body 221 is assembled at one end of the first driver 222 and is located on the first slide rail unit 21, and the The first driver 222 is arranged on the side of the first slide rail unit 21, and the second body 223 is assembled on the first body 221, and the second body 223 is also equipped with the second slide rail unit 224 on one side. The first arm 225 and the second arm 226 are respectively slidably arranged on the opposite sides of the second slide rail unit 224, and one end of the first arm 225 is assembled with the first clamp 227 and the other end is assembled on one end of the second driver 229, and one end of the second arm 226 is assembled with the second clamp 228 and the other end is assembled on the other end of the second driver 229. The second driver 229 is assembled on the second body 223, and the second driver 229 is provided with a rotation driving block 2291, a A first connecting rod 2292 and a second connecting rod 2293, one end of the first connecting rod 2292 is connected to the first arm 225 to move along the axial direction of the second slide rail unit 224, and the other end is pivotally connected to one end of the rotation driving block 2291, and one end of the second connecting rod 2293 is connected to the second arm 226 to move along the axial direction of the second slide rail unit 224, and the other end is pivotally connected to the other end of the rotation driving block 2291, and the other end of the first clamp 227 relative to the first arm 225 is respectively provided with a first locking groove 2271, and the other end of the second clamp 228 relative to the second arm 226 is respectively provided with a second locking groove 2281, wherein the first driver 222 controls the second body 2 23 moves along the axial direction of the first driver 222, and the first driver 222 controls a first distance D1 between the second body 223 and the first body 221, and the second driver 229 controls a second distance D2 between the first clamp 227 and the second clamp 228; in addition, after the clamping drive unit 22 clamps a centrifuge tube carrying module 10, the rotating drive block 2291 of the second driver 229 shortens the second distance D2, and the first retaining groove 2271 is limited and retained in the first retaining hole 12 of the clamped centrifuge tube carrying module 10, and the second retaining groove 2281 is limited and retained in the second retaining holes 13 of the clamped centrifuge tube carrying module 10;

[0062] The plurality of temperature control modules 30, each of which is provided with a plurality of second accommodating holes 31 and a temperature control unit 32, wherein the temperature control unit 32 enables the plurality of temperature control modules 30 to reach the desired reaction temperature and temperature change mode. The plurality of temperature control modules 30 of this embodiment are provided with four groups of the temperature control units 32 of the temperature control modules 30 as heaters, so that the temperature of the four groups of the temperature control modules 30 is higher than the room temperature, and the temperature control unit 32 of one group of the temperature control modules 30 is used as a cooler, so that the temperature of the group of temperature control modules 30 is equivalent to zero degrees Celsius, and the temperature control units 32 of two other groups of the temperature control modules 30 are thermostats, so that the temperature of the two groups of temperature control modules 30 is equivalent to the room temperature, but not limited thereto;

[0063] The capping module 40 is assembled at one end of the first slide rail unit 21, and the capping module 40 is provided with a cover body 41, a third driver 42 and two auxiliary rods 43. The third driver 42 is arranged on the peripheral side of the first slide rail unit 21, and one end of the third driver 42 is assembled with the cover body 41. The auxiliary rods 43 are arranged adjacent to the third driver 42 and one end is respectively assembled with the cover body 41. When the third driver 42 lifts and moves the cover body 41, the auxiliary rods 43 can assist the cover body 41 in lifting and lowering, thereby extending the operating life of the third driver 42. The cover body 41 is mainly used to cover the temperature control module 30 which is a heater of the temperature control unit 32, so as to prevent the liquid in the centrifuge tube 200 from evaporating when the temperature control module 30 is heated, but the invention is not limited thereto.

[0064] The magnetic field module 50 is assembled around one of the multiple temperature control modules 30. Further, the magnetic field module 50 is provided with a fourth driver 51, two telescopic rods 52 and a magnetic field generator 53. The fourth driver 51 is assembled around the first slide rail unit 21, and one end of the telescopic rod 52 is assembled with the fourth driver 51, and the other end is assembled with the magnetic field generator 53. The fourth driver 51 controls the length of the telescopic rod 52 to adjust the distance between the magnetic field generator 53 and the temperature control module 30. In addition, the magnetic field generator 53 of the magnetic field module 50 of this embodiment is arranged on the lower side of the temperature control module 30. The magnetic field generator 53 can also be arranged around the temperature control module 30, and can be customized with specific antibody magnetic beads (not shown) to provide the magnetic field generator 53 with purification of specific proteins for extraction or reverse transcription; and

[0065] The automated processing module 60 is electrically connected to the transport module 20, the temperature control modules 30, the capping module 40 and the magnetic field module 50;

[0066] The automatic processing module 60 controls the temperature control units 32 of the plurality of temperature control modules 30, and the plurality of temperature control modules 30 make the temperature control units 32 reach the set reaction temperature or temperature change mode respectively, and controls the clamping drive unit 22 of the transport module 20 to clamp a centrifuge tube carrying module 10, so that the centrifuge tube carrying module 10 moves along the first slide rail unit 21, and makes the centrifuge tube carrying module 10 selectively move to the temperature control module 30 with the magnetic field module 50 for nucleic acid extraction, and the nucleic acid extraction is mainly for DNA (deoxyribonucleic acid: deoxyribonucleic acid, DNA), RNA (ribonucleic acid: ribonucleic acid, RNA) or molecular markers (Molecular The centrifuge tube carrying module 10 is clamped and placed in the plurality of temperature control modules 30 with different temperatures in turn for nucleic acid amplification, or the centrifuge tube carrying module 10 is clamped and placed in one of the temperature control modules 30 to add a reagent containing a primer to mark all primers on the specimen for primer marking, or the centrifuge tube carrying module 10 is clamped and moved to the temperature control module 30 with the magnetic field module 50 for nucleic acid extraction, or one of the temperature control modules 30 is used to add a reagent containing a primer to mark all primers on the specimen for primer marking, or the centrifuge tube carrying module 10 is clamped and moved to the temperature control module 30 with the magnetic field module 50 for nucleic acid extraction, and then the centrifuge tube carrying module 10 is clamped and moved to the temperature control module 30 with the magnetic field module 50 for nucleic acid extraction, and then the centrifuge tube carrying module 10 is clamped and placed in the plurality of temperature control modules 30 with different temperatures in turn for nucleic acid amplification, or the centrifuge tube carrying module 10 is clamped and placed in one of the temperature control modules 30 to add a reagent containing a primer to mark all primers on the specimen for primer marking, The carrier module 10 is placed in turn in the plurality of temperature control modules 30 with different temperatures for nucleic acid amplification, or the centrifuge tube carrier module 10 is first moved to the temperature control module 30 with the magnetic field module 50 for nucleic acid extraction, and then the centrifuge tube carrier module 10 is placed in turn in the plurality of temperature control modules 30 with different temperatures for high temperature denaturation, low temperature primer connection, and sequence extension to achieve amplification effect for nucleic acid amplification, and then one of the temperature control modules 30 is filled with a reagent containing primers to mark all primers on the specimen for primer marking; and the temperature control unit 32 makes the centrifuge tubes 200 placed in the second containing holes 31 of the plurality of temperature control modules 30 reach the set reaction temperature or temperature change mode respectively;When the clamping drive unit 22 places the centrifuge tube carrying module 10 on the temperature control module 30, the first receiving hole 11 is arranged opposite to the second receiving hole 31. In addition, reverse transcription can also be performed after the nucleic acid extraction. The reverse transcription is to first transcribe the DNA of the specimen to obtain mRNA (messenger RNA: messenger RNA, mRNA), and then the extracted mRNA is reversely transcribed into cDNA (complementary deoxyribonucleic acid: complementary DNA, cDNA) through Oligo (dT) (polythymine, T repeat oligonucleotide: Oligo (dT)) and reverse transcriptase. Oligo (dT) is a nucleotide chain composed of thymine to produce only the required cDNA. ;

[0067] Furthermore, please see Figures 3 to 4-5 As shown, the user places the sample to be extracted in the centrifuge tube 200, and places the centrifuge tube 200 with the sample placed therein and subjected to the same reaction temperature or temperature change procedure in the first receiving hole 11 of the same centrifuge tube supporting module 10, and can add the required reagents in the centrifuge tube 200 to react with the sample, and the centrifuge tube supporting module 10 with the centrifuge tube 200 placed in the first receiving hole 11 can be placed in the temperature control module 30 first, and the user can set the automatic processing module 60 according to the requirements, please refer to Figure 1 As shown, the multiple temperature control modules 30 from left to right in this embodiment are respectively the temperature control module 30 in which the temperature control unit 32 is a cooler, two groups of the temperature control modules 30 in which the temperature control unit 32 is a thermostat and is maintained at room temperature, and one of the two groups of the temperature control modules 30 is also provided with the magnetic field module 50, and four groups of the temperature control modules 30 in which the temperature control unit 32 is a heater, and the four groups of the temperature control modules 30 not only have different temperatures from each other but are all higher than room temperature, and three of the groups of the temperature control modules 30 can be covered with the cover body 41 by the cover module 40, please refer to Figures 4 to 5. Figure 5As shown, the automated processing module 60, on the one hand, enables the multiple temperature control modules 30 to control the corresponding temperature control units 32 to reach a preset reaction temperature or a temperature change mode to be performed, and on the other hand, controls the second driver 229 of the clamping drive unit 22, and the second driver 229 controls the first support arm 225 and the second support arm 226 to move axially along the second slide rail unit 224, so that the second distance D2 between the first support arm 225 and the second support arm 226 is gradually shortened, thereby causing the first retaining groove 2271 of the first clamp 227 to be retained in the first retaining hole 12 of the centrifuge tube supporting module 10 to be moved, and the second retaining groove 2281 of the second clamp 228 to be retained in the first retaining hole 12 of the centrifuge tube supporting module 10 to be moved. The second buckle hole 13, that is, the second driver 229 will control the rotating drive block 2291 to rotate clockwise or counterclockwise, so that the rotating drive block 2291 drives the first connecting rod 2292 and the second connecting rod 2293 pivoted at both ends, so that the first connecting rod 2292 will drive the first support arm 225 connected to the other end to move axially along the second slide rail unit 224, and the second connecting rod 2293 will also drive the second support arm 226 connected to the other end to move axially along the second slide rail unit 224. By changing the length of the second distance D2, the first clamp 227 and the second clamp 228 of the clamping drive unit 22 can clamp a centrifuge tube supporting module 10 and make the centrifuge tube supporting module 10 move axially along the first slide rail unit 21.

[0068] Also, please refer to Figure 4-3 to Figure 4-5As shown, after the first clamp 227 and the second clamp 228 of the clamping drive unit 22 clamp a centrifuge tube carrying module 10, the automatic processing module 60 will control the clamping drive unit 22 to make the centrifuge tube carrying module 10 move along the axial direction of the first slide rail unit 21. When the centrifuge tube carrying module 10 moves to a corresponding temperature control module 30, the automatic processing module 60 will control the first driver 222, and the first driver 222 makes the second body 223 move toward the first body 221, that is, the first distance D1 between the second body 222 and the first body 221 is shortened, so that the centrifuge tubes 200 in the first accommodating hole 11 are placed one by one into the corresponding second accommodating hole 31; in addition, when the automatic processing module 60 controls the temperature control unit 32 to place the plurality of temperature control modules 30 to a predetermined After the reaction temperature or temperature change mode is set, the automatic processing module 60 will clamp the corresponding centrifuge tube carrying module 10 according to the setting, so that the specimen in the centrifuge tube 200 of the centrifuge tube carrying module 10 can only be moved to the temperature control module 30 with the magnetic field module 50, and the centrifuge tubes 200 are placed one by one in the corresponding second receiving holes 31, and the automatic processing module 60 will control the fourth driver 51 of the magnetic field module 50, so that the telescopic rod 52 will position the magnetic field generator 53 around the corresponding centrifuge tubes 200, so as to perform magnetic bead extraction on the specimen in the centrifuge tube 200, wherein the magnetic field generator 53 can be arranged below the temperature control module 30, or be arranged around the periphery of the temperature control module 30. In this embodiment, the magnetic field generator 53 is arranged below the temperature control module 30, but is not limited thereto.

[0069] Furthermore, when the user only needs to perform nucleic acid amplification, the automatic processing module 60 will clamp the corresponding one of the centrifuge tube carrying modules 10 according to the setting, so that the sample in the centrifuge tube 200 of the centrifuge tube carrying module 10 is placed in the second receiving hole 31 of the temperature control module 30 corresponding to the reaction temperature, and the sample in the centrifuge tube 200 reaches the required reaction temperature and thus reaches the required nucleic acid quantity; furthermore, if the user wants to perform primer labeling, the automatic processing module 60 will clamp the corresponding one of the centrifuge tube carrying modules 10 according to the setting, and place one of the The second receiving hole 31 of the temperature control module 30 is used to add the reagent containing the primer into the centrifuge tube 200 for primer labeling; further, the user can also input the command into the automatic processing module 60 according to the demand, so that the automatic molecular operation system 100 can not only perform nucleic acid extraction, nucleic acid amplification, primer labeling or reverse transcription on liquid specimens such as blood, saliva, urine or cerebrospinal fluid alone, but also perform primer labeling after nucleic acid extraction, or perform nucleic acid amplification first after nucleic acid extraction and then primer labeling, etc. Furthermore, in order to prevent the reagent added in the centrifuge tube 200 from escaping due to the high temperature during the heating process, the capping module 40 is set at the temperature control module 30 where the temperature control unit 32 is a heater, so that when the centrifuge tube carrying module 10 places the centrifuge tube 200 in the temperature control module 30 where the temperature control unit 32 is a heater, the automatic processing module 60 controls the third driver 42, and the third driver 42 makes the cover body 41 cover the centrifuge tube 200 of the centrifuge tube carrying module 10, and waits to be moved to other temperature control modules 30 Or when performing subsequent extraction operations, the cover 41 is raised again; therefore, the automated molecular operating system 100 automatically moves the centrifuge tube carrying module 10 by the automated processing module 60 according to the set process, so that the centrifuge tube 200 reaches the required reaction temperature or temperature change mode in the corresponding multiple temperature control modules 30, and then automatically completes the required temperature change and number of cycles, thereby achieving the required nucleic acid extraction, nucleic acid amplification, primer labeling, reverse transcription or a combination thereof, reducing errors caused by manual operation and increasing convenience.

[0070] In summary, the automated molecular operating system 100 of the present invention is mainly controlled by the automated processing module 60 to control the clamping drive unit 22 of the transport module 20, so that the clamping drive unit 22 clamps a centrifuge tube carrying module 10 so that it can move axially along the first slide rail unit 21 according to the set mode, so that the specimen in the centrifuge tube 200 placed in the first receiving hole 11 of the centrifuge tube carrying module 10 can be automatically moved to the temperature control module 30 with the magnetic field module 50 for nucleic acid extraction, or placed in the multiple temperature control modules 30 with different temperatures in turn for nucleic acid amplification, or placed in one of the temperature control modules 30 to fill with a reagent containing a primer for primer labeling, or placed in the temperature control module 30 with the magnetic field module 50 before the sample is extracted. After nucleic acid extraction, it is selected according to needs whether to place the sample in the plurality of temperature-controlled modules 30 with different temperatures in turn for nucleic acid amplification, or whether to place the sample in one of the temperature-controlled modules 30 after nucleic acid amplification to add reagents containing primers for primer labeling or reverse transcription and other biological procedures. In this way, the user can perform nucleic acid extraction, nucleic acid amplification, primer labeling, reverse transcription or a combination thereof in an automated manner in the automated molecular operating system 100 according to needs, that is, perform nucleic acid amplification or a combination of nucleic acid amplification and primer labeling according to needs after nucleic acid extraction. In this way, the sample can be transferred and reacted in an automated manner in the same system environment, which not only reduces labor costs, but also avoids and reduces errors caused by manual operations and increases the convenience of operation.

Claims

1. An automated molecular operating system, characterized in that: The centrifuge tube containing the specimen is used for nucleic acid extraction, nucleic acid amplification, primer labeling or reverse transcription. The automated molecular operating system mainly includes: At least one centrifuge tube supporting module, each of which is provided with a plurality of first receiving holes, at least one first buckle hole and at least one second buckle hole, wherein the plurality of first receiving holes are provided between the first buckle hole and the second buckle hole; A transport module, which has a first slide rail unit and a clamping drive unit, wherein the clamping drive unit is assembled on the first slide rail unit, and the clamping drive unit clamps a centrifuge tube carrying module and moves along the axial direction of the first slide rail unit; A plurality of temperature control modules, each of which is provided with a plurality of second accommodating holes and a temperature control unit, wherein the temperature control unit enables the plurality of temperature control modules to reach a desired reaction temperature and temperature change mode; A cover module is assembled at one end of the first slide rail unit and is provided with a cover body; a magnetic field module, which is assembled around one of the plurality of temperature control modules; and an automated processing module electrically connected to the transport module, the temperature control modules, the capping module and the magnetic field module; The automatic processing module controls the temperature control units of the plurality of temperature control modules to reach the set reaction temperature or temperature change mode respectively, and controls the clamping drive unit of the transport module to clamp one of the centrifuge tube carrying modules, so that the centrifuge tube carrying module moves along the first slide rail unit, and the centrifuge tube carrying module is selectively moved to the temperature control module with the magnetic field module for nucleic acid extraction, or is alternately placed in the plurality of temperature control modules with different temperatures for nucleic acid amplification, or is placed in one of the temperature control modules for adding a reagent containing a primer for primer labeling; Wherein, when the clamping driving unit places a centrifuge tube carrying module on a temperature control module, the plurality of first accommodating holes are arranged opposite to the plurality of second accommodating holes.

2. The automated molecular manipulation system according to claim 1, characterized in that: Furthermore, the clamping drive unit is provided with a first body, a first driver, a second body, a second slide rail unit, a first support arm, a second support arm, a first clamp, a second clamp and a second driver, and the first body is assembled at one end of the first driver and is located on the first slide rail unit, and the first driver is arranged on the periphery of the first slide rail unit, and the first body is assembled with the second body above it, and the second body is provided with the second slide rail unit on a side, and the first support arm and the second support arm are respectively slidably arranged on the second On opposite sides of the slide rail unit, one end of the first arm is assembled with the first clamp and the other end is assembled with one end of the second driver, and one end of the second arm is assembled with the second clamp and the other end is assembled with the other end of the second driver, and the second driver is assembled on the second body, wherein the first driver controls the axial movement of the second body along the first driver, and enables the first driver to control a first distance between the second body and the first body, and the second driver controls a second distance between the first clamp and the second clamp.

3. The automated molecular manipulation system according to claim 2, characterized in that: Furthermore, the second driver is provided with a rotating drive block, a first connecting rod and a second connecting rod, one end of the first connecting rod is connected to the first support arm for axial movement along the second slide rail unit, and the other end is pivotally connected to one end of the rotating drive block, and one end of the second connecting rod is connected to the second support arm for axial movement along the second slide rail unit, and the other end is pivotally connected to the other end of the rotating drive block.

4. The automated molecular manipulation system according to claim 2, characterized in that: Furthermore, the other end of the first clamp relatively assembled on the first support arm is respectively provided with a first locking groove, and the other end of the second clamp relatively assembled on the second support arm is respectively provided with a second locking groove, wherein after the clamping drive unit clamps a centrifuge tube carrying module, the rotating drive block of the second driver shortens the second distance, and the first locking groove is limited and locked to the first locking hole of the clamped centrifuge tube carrying module, and the second locking groove is limited and locked to the first locking hole and the second locking hole of the clamped centrifuge tube carrying module.

5. The automated molecular manipulation system according to claim 1, wherein: Furthermore, the covering module is provided with a third driver and at least one auxiliary rod. The third driver is arranged on the peripheral side of the first slide rail unit, and one end of the third driver is assembled with the cover body. The auxiliary rod is adjacent to the third driver and one end is respectively assembled with the cover body.

6. The automated molecular manipulation system according to claim 1, wherein: Furthermore, the magnetic field module is provided with a fourth driver, at least one telescopic rod and a magnetic field generator. The fourth driver is assembled on the periphery of the first slide rail unit, and one end of the telescopic rod is assembled on the fourth driver, and the other end is assembled with the magnetic field generator. The fourth driver controls the length of the telescopic rod to adjust the distance between the magnetic field generator and the temperature control module.

7. The automated molecular manipulation system according to claim 1, wherein: The automated processing module controls the clamping drive unit to clamp a centrifuge tube carrying module and move it first to the temperature control module with the magnetic field module for nucleic acid extraction, and then add a reagent containing a primer to one of the temperature control modules for primer labeling, or clamp a centrifuge tube carrying module and move it first to the temperature control module with the magnetic field module for nucleic acid extraction, and then clamp the centrifuge tube carrying module and place it in turn in the multiple temperature control modules with different temperatures for nucleic acid amplification, or clamp a centrifuge tube carrying module and move it first to the temperature control module with the magnetic field module for nucleic acid extraction, and then clamp the centrifuge tube carrying module and place it in turn in the multiple temperature control modules with different temperatures for nucleic acid amplification, and then add a reagent containing a primer to one of the temperature control modules for primer labeling.

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