Bactericidal composition for preventing and treating fig rust disease

Abstract

The invention belongs to the technical field of bactericides, and particularly relates to a bactericidal composition for preventing and treating fig rust. The invention relates to a bactericidal composition, which is used for preventing and treating fig rust disease, and the bactericidal composition is prepared by compounding trifluoropyridylamine and flusilazole or 1-(2-hydroxyphenyl)-1-butanone. According to the bactericidal composition, the trifluoropyridinamine and the flusilazole or the 1-(2-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1-hydroxyphenyl)-1-(1- meanwhile, generation and development of drug resistance of pathogenic bacteria can be delayed, and the drug resistance risk of the bactericidal composition is reduced. And secondly, the bactericidal composition disclosed by the invention can broaden the compounding combination of the trifluoropyridylamine, and provides support for fully developing the compounding medicament of the trifluoropyridylamine.

Description

Technical Field

[0001] The invention belongs to the technical field of fungicides, and particularly relates to a fungicide composition for preventing and treating fig rust. Background Art

[0002] Fig rust is prone to occur in rainy years and in humid orchards in greenhouses. The disease first infects the base of the leaves and gradually spreads upward. The early symptom is rust-colored leaf veins. As the disease becomes more serious, reddish-brown spots appear on the front of the leaves. The spots gradually expand and connect into pieces when they are serious. A large number of rust-colored powdery spores are produced on the back of the diseased leaves. Shaking the trees or branches in orchards where rust is prevalent can see the spores spread in the air like smoke. When fig rust occurs, it will cause early leaf fall of figs and occur together with deadwood disease, affecting yield and quality.

[0003] The pathogen of fig rust is Uredinales imperfecti, which only has summer spore generation, but no rust spore and winter spore generation. It belongs to Uredinales imperfecti. Summer spore piles grow on the back of leaves and are brown; summer spores are yellow to yellow-brown, with warty thorns and thick walls. In the production of figs, chemical control is mainly used for fig rust, and methyl thiophanate, tebuconazole, difenoconazole, triadimefon, diniconazole and myclobutanil can be sprayed. However, in the process of chemical control of pests and diseases, drug resistance is an issue that cannot be ignored. With the long-term and large-scale unscientific use of drugs, the fig rust pathogen has developed different degrees of drug resistance to various existing drugs, resulting in a year-on-year decrease in the control effect.

[0004] Triflupyridam is a fungicide and nematicide of the succinate dehydrogenase inhibitor (SDHI) class. In terms of pathogen control, triflupyridam is a pathogen respiration inhibitor. It interferes with the tricarboxylic acid cycle on the respiratory electron transport chain complex II, inhibits the function of mitochondria, prevents them from producing energy, and then inhibits the growth of pathogens, eventually leading to the death of pathogens; in terms of nematode control, it inhibits the activity of succinate dehydrogenase and destroys the production of ATP in the mitochondria of plant pathogenic nematodes, and can be used to prevent and control a variety of fungal diseases and nematodes. Summary of the invention

[0005] The purpose of the present invention is to provide a fungicide composition for preventing and controlling fig rust. In the process of chemical prevention and control of pests and diseases, drug resistance is a problem that cannot be ignored. With the long-term, large-scale and unscientific use of agents, fig rust pathogens have developed different degrees of drug resistance to various existing agents, resulting in a problem that the prevention and control effect is also reduced year by year.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A fungicide composition is used for preventing and treating fig rust. The fungicide composition is prepared by compounding trifluopyramide with flusilazole or fenclofenone.

[0008] Furthermore, the mass ratio of trifluoropyridinamine to flusilazole is 1-40:15-1.

[0009] Furthermore, the mass ratio of triflupyridamole to fenclofenone is 1-20:20-1.

[0010] A bactericide, comprising the bactericidal composition, wherein the mass of the bactericidal composition accounts for 0.75-55% of the total mass of the bactericide.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] (1) The present invention compounds triflupyramide with flusilazole or fenclofenone in a certain mass ratio. The compound combination shows a synergistic effect on the fig rust pathogen Uergysporium ficifolium within a certain mass ratio range. Compared with a single component, the control effect on fig rust can be improved, and the generation and development of pathogen resistance can be delayed, thereby reducing the resistance risk of the fungicidal composition.

[0013] (2) The fungicidal composition of the present invention can broaden the compound combinations of trifluopyridine and provide support for the full development of compound agents of trifluopyridine. DETAILED DESCRIPTION

[0014] The following is a clear and complete description of the technical solution of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0015] Example :Indoor biological activity test of trifluopyramide complex against fig rust

[0016] 1. Test strains

[0017] Fresh uredospores of fig uredospore rust were collected from fig leaves with typical symptoms.

[0018] 2. Test reagents

[0019] 86% triflupyridamole technical (Syngenta Crop Protection Co., Ltd., Switzerland), 95% flusilazole technical (Shandong Weifang Runfeng Chemical Co., Ltd.), 98% fenclofenone technical (Hubei Hengjingrui Chemical Co., Ltd.)

[0020] The test agent was first dissolved in dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple groups of proportions were set, and each single dose and proportioned mixture was set with 5 mass concentration gradients according to the equal ratio method.

[0021] 3. Test method (refer to "NY / T 1156.1-2006 Pesticide Indoor Bioassay Test Guidelines Fungicides Part 1: Inhibition of Pathogenic Fungal Spore Germination Test Concave Slide Method")

[0022] Collect fresh summer spores of Fig Summer Rust Fungus on fig leaves and resuspend the spores to 1×10 7 / mL, and add 0.5% glucose solution. Take 0.5mL of the agent and 0.5mL of the spore suspension and mix them evenly, then use a micropipette to drop the above mixture onto a concave glass slide, place it in a culture dish with a shallow layer of water, cover it and place it in a 25℃ incubator. Set up 4 replicates for each treatment, and set the treatment with 0.1% Tween-80 aqueous solution as a blank control.

[0023] When the spore germination rate of the blank control reached more than 90%, the spore germination of each treatment was checked. Three fields of view were randomly observed for each treatment and each repetition, and a total of 200 spores were investigated. The number of germination and the total number of spores were recorded respectively. The spore germ tube length greater than the short radius of the spore was considered to be germinated. According to the investigation results, the relative inhibition rate of spore germination of each treatment was calculated.

[0024]

[0025] 4. Data Analysis

[0026] DPS software was used for data statistical analysis. The logarithmic value of fungicide concentration was taken as x and the corresponding relative inhibition rate of spore germination probability value was taken as y for linear regression to obtain the EC value of the toxicity of the agent to the target pathogen. 50 The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0027] 5. Measurement results

[0028] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0029] Table 1 Indoor bioactivity test of trifluopyramide and flusilazole against fig rust

[0030] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Trifluopyridine 0.9357 100.0000 -- -- Flusilazole 12.7031 7.3659 -- -- Trifluopyridine 1: Flusilazole 15 5.6311 16.6166 13.1555 126.3090 Trifluopyridine 1: Flusilazole 10 3.0037 31.1516 15.7872 197.3218 Trifluopyridine 1: Flusilazole 5 3.3702 27.7639 22.8049 121.7453 Triflupyridine amine 1: Flusilazole 1 1.0531 88.8520 53.6830 165.5124 Triflupyridine 5: Flusilazole 1 0.7413 126.2242 84.5610 149.2700 Triflupyridine amine 10: Flusilazole 1 0.5462 171.3109 91.5787 187.0641 Triflupyridine 20: Flusilazole 1 0.8075 115.8762 95.5889 121.2235 Trifluopyridine 30: Flusilazole 1 0.6239 149.9760 97.0118 154.5956 Trifluopyridine 40: Flusilazole 1 0.7774 120.3627 97.7406 123.1450

[0031] As shown in Table 1, after trifluopyridine and flusilazole were compounded, the co-toxicity coefficients against fig uremic rust were all greater than 120 within the mass ratio of 1-40:15-1, showing a synergistic effect.

[0032] Table 2 Indoor bioactivity test of triflupyridamole and fenclofenone against fig uremic rust

[0033]

[0034]

[0035] As shown in Table 2, after triflupyridamole and fenclofenac were compounded, the co-toxicity coefficients against Uergyps syringae pv. figi were all greater than 120 within the mass ratio of 1-20:20-1, showing a synergistic effect.

[0036] In summary, the present invention compounds triflupyridamole with flusilazole or fenclofenone in a certain mass ratio, and the compound combination shows a synergistic effect on the fig rust pathogen Uergystis syringae within a certain mass ratio range. Compared with a single component, the control effect on fig rust can be improved, and the generation and development of pathogen resistance can also be delayed, thereby reducing the drug resistance risk of the fungicidal composition.

Claims

1. A fungicidal composition for preventing and treating fig rust, characterized in that: The bactericidal composition is prepared by compounding trifluopyramide with flusilazole or fenclofenone.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of trifluoropyridinamine to flusilazole is 1-40:15-1.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of trifluopyramide to fenclofenone is 1-20:20-1.

4. A bactericide, characterized in that: The bactericide comprises the bactericidal composition according to claim 1, and the mass of the bactericidal composition accounts for 0.75-55% of the total mass of the bactericide.

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