Barrier repairing oil-soluble lip care composition containing multiple recombinant collagens and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to an oil-soluble lip care composition containing multiple recombinant collagens and capable of repairing a barrier and a preparation method of the oil-soluble lip care composition. The composition is prepared from the following raw materials in parts by weight: 0.01 to 0.5 part of recombinant collagen, 0.05 to 1 part of fermented plant extract, 20 to 40 parts of grease and 10 to 20 parts of polyhydric alcohol. The composition disclosed by the invention contains various active ingredients, can effectively repair a lip skin barrier, has relatively good effects of resisting aging, preserving moisture and resisting influence of external factors, has low irritation and high safety, and also has relatively good effects of moistening and moisturizing lips and smoothing the lips.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to an oil-soluble lip care composition for repairing a barrier containing multiple recombinant collagens and a preparation method thereof. Background Art

[0002] The thickness of the lip skin is only one-third of the skin on other parts of the body; this causes the lip skin to evaporate faster than other parts of the body, and is more susceptible to external environmental stimuli (such as germs, dust, ultraviolet rays, or chemicals). If the lip skin is not properly cared for, it may cause dead skin to accumulate, crack, fine lines at the corners of the lips, and bacterial infection; therefore, it is necessary to use appropriate lip care products to care for the lips in a timely manner.

[0003] Currently, there are many types of lip care compositions with varying quality, and most of them are water-based products, which cannot effectively isolate the lips from the influence of the external environment and are difficult to effectively care for the lips. Therefore, it is necessary to provide an oil-soluble composition for effectively caring for the lips. Summary of the invention

[0004] In view of the above technical problems, the present invention proposes a technical solution of the present invention, which includes an oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens and a preparation method thereof.

[0005] On the one hand, the present invention provides an oil-soluble lip care composition for repairing the barrier containing multiple recombinant collagens, which comprises the following raw materials in parts by mass: 0.01-0.5 parts of recombinant collagen, 0.05-1 parts of fermented plant extracts, 20-40 parts of oils and 10-20 parts of polyols.

[0006] Preferably, the following raw materials are included in parts by mass: 0.02-0.3 parts of recombinant collagen, 0.06-0.8 parts of fermented plant extract, 22-38 parts of oil and fat, and 12-18 parts of polyol.

[0007] Preferably, the recombinant collagen includes recombinant collagen type I (recombinant humanized type I collagen), type III (recombinant humanized type III collagen), and type XVII (recombinant humanized type XVII collagen).

[0008] The structure of recombinant humanized type I collagen is similar to that of natural collagen, with a triple helix structure that can automatically twist and fold to form a stable collagen triple helix structure. This structure enables recombinant collagen to effectively replenish and repair collagen in the skin, thereby achieving anti-wrinkle and moisturizing effects.

[0009] Recombinant humanized type III collagen has a clear triple helix structure, and its amino acid sequence is consistent with the specific functional area of ​​the human body's own collagen amino acid sequence. It has excellent compatibility and will not cause rejection. Recombinant type III humanized collagen has a two-way regulation, repair and regeneration function. It not only supplements the missing type III collagen in the skin, but also repairs damaged and aged fibroblasts, and continues to secrete type I collagen, making the skin firm, elastic, plump and supple. Its unique active triple helix network structure contains many water molecules. The interaction between adjacent triple helices forms dense and regular collagen fibers. A large number of hydrophilic amino acids tightly adsorb a large number of water molecules to generate bound water, which ensures the active metabolic function of the cells and keeps the skin supple.

[0010] Recombinant humanized collagen type XVII is a type of collagen prepared by genetic engineering technology. It has an amino acid sequence that is completely consistent with the body's own collagen, has good biocompatibility, and has no immune rejection reaction. This type of collagen has significant effects on skin anti-aging, repair, and regeneration, and because of its unique structure and function, it has broad application prospects in the fields of medicine and beauty. It has excellent cell adhesion and migration capabilities, which helps promote the regeneration and repair of skin cells; its excellent elasticity and pressure resistance can provide better support and protection for the skin; its moisturizing effect can provide sufficient moisture for the skin to prevent dryness and aging; and recombinant humanized collagen type XVII has high biocompatibility and low immunogenicity, and is highly safe.

[0011] Preferably, the mass ratio of recombinant collagen type I, type III and type XVII is 1:(0.5-1.2):(0.5-1.2).

[0012] Preferably, the oils and fats include synthetic esters and vegetable oils.

[0013] Preferably, the synthetic ester includes at least one of caprylic / capric triglyceride, cetyl lactylate, myristyl lactylate, trimyristin, castor oil fatty acid methyl ester, and olein.

[0014] Preferably, the vegetable oil includes at least one of avocado oil, camellia oil, jojoba oil, soybean oil, palm oil, almond oil and coconut oil.

[0015] Preferably, the polyol includes at least one of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, butylene glycol, and sorbitol.

[0016] Preferably, the raw materials for preparing the fermented plant extract include at least one of Gentiana macrophylla, Dipsacus asper, Glehnia littoralis leaf, and Crab shell.

[0017] Another aspect of the present invention provides a method for preparing the composition, comprising the following steps:

[0018] Step 1: Heat and melt the oil, add the polyol, and stir to mix to obtain a mixture 1;

[0019] Step 2: Cooling the mixture 1, adding the fermented plant extract, stirring and mixing, to obtain a mixture 2;

[0020] Step 3: Cool the mixture 2, add recombinant collagen, and stir to mix, so as to obtain the barrier-repairing oil-soluble lip care composition containing multiple recombinant collagens.

[0021] Preferably, in step 1, the melting temperature is 80-95° C. and the stirring time is 0.2-1 hour.

[0022] Preferably, in step 2, the temperature is lowered to 40-55° C. and the stirring time is 0.2-1 hour.

[0023] Preferably, in step 3, the temperature is lowered to 25-35° C. and the stirring time is 0.2-1 hour.

[0024] Preferably, in step 3, after stirring and mixing, sterilization and packaging steps are also included.

[0025] The method for preparing the aforementioned fermented plant extract comprises:

[0026] S1: crush the raw material to less than 80 mesh to obtain powder, add 15-40 times the weight of the powder into water, boil, then stir at 60-80℃ for 2-5 hours, cool, and obtain product 1;

[0027] S2: adding serrapeptase and snail protease to product 1, performing enzymolysis at 40-60°C for 2-5 hours, and then inactivating the enzyme to obtain product 2; wherein the amounts of serrapeptase and snail protease added are 300-500 U / g product 1 and 200-450 U / g product 1, respectively;

[0028] S3: Product 2 is sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria are added, and the mixture is fermented anaerobically at 30-40°C for 95-155 hours. After the fermentation is completed, the mixture is sterilized to obtain Product 3; wherein the glucose and potassium dihydrogen phosphate are 0.5-3.75% and 0.005-0.08% of the mass of Product 2, and the amount of fermentation bacteria added is 2.7×10 8 -8.2×10 8 cfu / g product 2, the fermentation bacteria consisted of Paenibacillus polymyxa CICC23712 and Lactobacillus reuteri CICC6126 in a quantitative ratio of 1:0.2-0.8;

[0029] S4: Product 3 is concentrated under reduced pressure to 0.5-1.5 times the mass of the powder in S1, and then anhydrous ethanol is added to adjust the ethanol mass concentration to 85-95%, and heated and stirred at 40-60°C for 2-4 hours to obtain product 4;

[0030] S5: The product 4 is filtered to obtain a filtrate, the filtrate is subjected to ultrafiltration treatment to obtain an ultrafiltrate, the ultrafiltrate is concentrated under reduced pressure, and dried to obtain the fermented plant extract.

[0031] Preferably, the raw materials include Gentiana macrophylla, Dipsacus asper (Chuanxiongduan), and Glehnia littoralis leaves.

[0032] Preferably, the raw materials may include 0.1-2 parts of Gentiana macrophylla, 0.2-2 parts of Dipsacus asper and 0.1-2 parts of Echinochloa macrophylla leaves in parts by mass.

[0033] Beneficial effects of the present invention:

[0034] The present invention uses recombinant collagen, fermented plant extracts, oils and polyols to prepare an oil-soluble composition. Type I, type III and type XVII of recombinant collagen give the composition excellent moisturizing, anti-aging and skin nutrition effects; the fermented plant extract gives the composition excellent free radical resistance, anti-aging and moisturizing effects; the oil component can effectively isolate the stimulating effect of external environmental factors on the sensitive skin of the lips, and give better resistance to the influence of external factors. The composition of the present invention contains various active ingredients, can effectively repair the lip skin barrier, has good anti-aging, moisturizing and external factor resistance effects, and has a better experience.

[0035] The extract prepared by the invention has a good free radical resistance effect, and gives the skin anti-aging and oxidation functions. After the extract is diluted to 1.5%, the scavenging rates of DPPH and ABTS free radicals reach 77.91% and 65.48%.

[0036] The present invention uses serrapeptase and snail protease for co-fermentation, which increases the types and richness of active ingredients in the extract, and gives the extract a better free radical scavenging effect. The two have a mutually reinforcing relationship in enhancing the free radical scavenging effect, and have a better effect than other proteases for enzymatic hydrolysis.

[0037] The extract prepared by using specific fermentation bacteria Paenibacillus polymyxa CICC23712 and Lactobacillus reuteri CICC6126 has a better free radical scavenging effect than extracts obtained from other fermentation bacteria, and can give the cosmetic composition excellent anti-aging function. The two have a synergistic relationship in enhancing the free radical scavenging effect, and when the ratio is 1:0.2-0.8, the obtained extract has a better effect.

[0038] The extract prepared by the present invention has a good moisturizing effect on the skin and can effectively make the lips plump and moist. The specific enzyme combination of the present invention makes the extract have a better effect than other enzymes. The combined enzymatic hydrolysis process of the present invention can give the extract a better moisturizing effect on the composition. The process of co-fermentation of the specific fermentation bacteria of the present invention makes the plant promote the moisturizing effect of the composition show significant improvement.

[0039] The lip care composition prepared by the invention has low irritation and high safety.

[0040] The lip care composition prepared by the present invention has good effect of moisturizing and hydrating lips and has a relatively delicate effect. The composition of the present invention has an excellent use experience and has a good use effect.

[0041] The preparation method of the invention is simple, the product effect is excellent, and it has good application prospect. DETAILED DESCRIPTION

[0042] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0043] Paenibacillus polymyxa CICC23712, Lactobacillus reuteri CICC6126, and Saccharomyces cerevisiae CICC1301 were purchased from China Industrial Microbiological Culture Collection Center.

[0044] 1. Preparation and testing of extracts

[0045] Extract 1:

[0046] S1: Preparation: 1 part of Gentiana macrophylla (whole plant), 0.9 part of Dipsacus asper (whole plant) and 0.8 part of Radix Glehniae leaf by weight; wherein the weight ratio of Gentiana macrophylla to Radix Glehniae leaf is 1:0.8;

[0047] The raw materials were crushed in a pulverizer to less than 80 meshes to obtain powder, and 27 times the weight of the powder was added with water, boiled, then cooled to 70°C, kept warm, stirred at 60 rpm for 3 hours, and cooled to room temperature to obtain product 1;

[0048] S2: Add serrapeptase and snail protease to product 1, stir and enzymolyze at 50°C and 70rpm for 3.3 hours, then inactivate the enzyme to obtain product 2; wherein the amount of serrapeptase and snail protease added is 400U / g product 1 and 300U / g product 1, respectively;

[0049] S3: Product 2 was sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria were added, and the mixture was fermented anaerobically at 35°C for 142 hours. After the fermentation was completed, the mixture was sterilized to obtain Product 3; wherein, glucose and potassium dihydrogen phosphate accounted for 2.87% and 0.045% of the mass of Product 2, and the amount of fermentation bacteria added was 6.60×10 8 cfu / g product 2, the fermentation bacteria consisted of Paenibacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a quantitative ratio of 1:0.5;

[0050] S4: Product 3 was concentrated under reduced pressure to 1.2 times the mass of the powder in S1, and then anhydrous ethanol was added to adjust the ethanol mass concentration to 90.5%, and stirred at 52°C and 65rpm for 2.5 hours to obtain product 4;

[0051] S5: The product 4 is filtered through a 0.5 micron filter membrane to obtain a filtrate, and the filtrate is ultrafiltered through an ultrafiltration membrane with a molecular weight cutoff of 2000Da to obtain an ultrafiltrate, and the ultrafiltrate is concentrated under reduced pressure at 50°C to 0.8 times the mass of the powder in S1, and freeze-dried at -40°C to a water content of 1.75% to obtain the fermented plant extract.

[0052] Extract 2:

[0053] S1: Prepare materials: 1.3 parts of Gentiana macrophylla (whole plant), 1 part of Dipsacus asper (whole plant) and 0.7 parts of Echinochloa macrophylla leaves by weight; wherein the weight ratio of Gentiana macrophylla to Echinochloa macrophylla leaves is 1:0.54;

[0054] The raw materials were placed in a pulverizer and crushed to less than 80 meshes to obtain powder, and 26.5 times the weight of the powder was added with water, boiled, and then cooled to 72°C, kept warm, stirred at 60 rpm for 3.2 hours, and cooled to room temperature to obtain product 1;

[0055] S2: Add serrapeptase and snail protease to product 1, stir and enzymolyze at 50°C and 70rpm for 3.5 hours, then inactivate the enzyme to obtain product 2; wherein the amount of serrapeptase and snail protease added is 300U / g product 1 and 400U / g product 1, respectively;

[0056] S3: Product 2 was sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria were added, and the mixture was fermented anaerobically at 36.5°C for 137 hours. After the fermentation was completed, the mixture was sterilized to obtain Product 3; wherein glucose and potassium dihydrogen phosphate accounted for 3.03% and 0.049% of the mass of Product 2, and the amount of fermentation bacteria added was 6.45×10 8 cfu / g product 2, the fermentation bacteria consisted of Paenibacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a quantitative ratio of 1:0.8;

[0057] S4: Product 3 was concentrated under reduced pressure to 1.2 times the mass of the powder in S1, and then anhydrous ethanol was added to adjust the ethanol mass concentration to 87.5%, and stirred at 52°C and 65rpm for 3 hours to obtain product 4;

[0058] S5: The product 4 is filtered through a 0.5 micron filter membrane to obtain a filtrate, and the filtrate is ultrafiltered through an ultrafiltration membrane with a molecular weight cutoff of 2000Da to obtain an ultrafiltrate, and the ultrafiltrate is concentrated under reduced pressure at 50°C to 0.8 times the mass of the powder in S1, and freeze-dried at -40°C to a water content of 1.71% to obtain the fermented plant extract.

[0059] Extract 3:

[0060] S2 is as follows: add bromelain to product 1, stir and enzymolyze at 50°C and 70rpm for 3.3 hours, then inactivate the enzyme to obtain product 2; wherein the amount of bromelain added is 700U / g product 1; and the rest are the same as extract 1.

[0061] Extract 4:

[0062] S2 is as follows: add serrapeptase to product 1, stir and enzymolyze at 50°C and 70rpm for 3.3 hours, then inactivate the enzyme to obtain product 2; wherein, the amount of serrapeptase added is 700U / g product 1; and the rest is the same as extract 1.

[0063] Extract 5:

[0064] S2 is: add snail protease to product 1, stir and hydrolyze at 50°C and 70rpm for 3.3 hours, then inactivate the enzyme to obtain product 2; wherein, the amount of snail protease added is 700U / g product 1; and the rest is the same as extract 1.

[0065] Extract 6:

[0066] S3 is as follows: Product 2 is sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria are added, and anaerobically fermented at 35°C for 142 hours. After the fermentation is completed, sterilization is performed to obtain Product 3; wherein glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of Product 2, and the amount of fermentation bacteria added is 6.60×10 8 cfu / g product 2, the fermentation bacteria is Bacillus polymyxa CICC23712; the others are the same as extract 1.

[0067] Extract 7:

[0068] S3 is as follows: Product 2 is sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria are added, and anaerobically fermented at 35°C for 142 hours. After the fermentation is completed, sterilization is performed to obtain Product 3; wherein glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of Product 2, and the amount of fermentation bacteria added is 6.60×10 8 cfu / g product 2, the fermentation bacteria is Lactobacillus reuteri CI CC6126; the rest are the same as extract 1.

[0069] Extract 8:

[0070] S3 is as follows: Product 2 is sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria are added, and anaerobically fermented at 35°C for 142 hours. After the fermentation is completed, sterilization is performed to obtain Product 3; wherein glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of Product 2, and the amount of fermentation bacteria added is 6.60×10 8 cfu / g product 2, the fermentation bacteria consisted of Paenibacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a quantitative ratio of 1:0.1; the rest were the same as extract 1.

[0071] Extract 9:

[0072] S3 is as follows: Product 2 is sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria are added, and anaerobically fermented at 35°C for 142 hours. After the fermentation is completed, sterilization is performed to obtain Product 3; wherein glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of Product 2, and the amount of fermentation bacteria added is 6.60×10 8 cfu / g product 2, the fermentation bacteria consisted of Paenibacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a quantitative ratio of 1:1.5; the rest were the same as extract 1.

[0073] Extract 10:

[0074] S3 is as follows: Product 2 is sterilized, and then glucose, potassium dihydrogen phosphate and fermentation bacteria are added, and anaerobically fermented at 35°C for 142 hours. After the fermentation is completed, sterilization is performed to obtain Product 3; wherein glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of Product 2, and the amount of fermentation bacteria added is 6.60×10 8 cfu / g product 2, the fermentation bacteria is Saccharomyces cerevisiae CICC1301; the others are the same as extract 1.

[0075] A. Antioxidant effect test:

[0076] 1. DPPH free radical scavenging ability test:

[0077] Weigh DPPH and dilute it with anhydrous ethanol to prepare 2×10 -4 mol / L DPPH solution was stored in a refrigerator away from light for future use.

[0078] Take 4.0mL of 2×10 -4 1 mol / L DPPH solution and 1.0 mL 95% ethanol solution were mixed, kept away from light for half an hour, and the absorbance was measured at 515 nm and recorded as A0.

[0079] Take 4.0 mL of 95% ethanol solution and 1.0 mL of the test sample (the extract is prepared by diluting 85% ethanol solution, and the extract concentration is 1.5%), mix well, keep it away from light for half an hour, and measure the absorbance at 515 nm, which is recorded as Ax.

[0080] Take 4.0mL of 2×10 -4 1 mol / L DPPH solution and 1.0 mL of the test sample (prepared by diluting the above extract with 85% ethanol solution, the extract concentration is 1.5%), mix well, keep it in the dark for half an hour, and measure the absorbance at 515 nm, which is recorded as Ay. Calculate the DPPH clearance rate T = [1-(Ay-Ax) / A0] × 100%.

[0081] 2. ABTS free radical scavenging ability test

[0082] Prepare ABTS into a 2 mmol / L solution with distilled water, take 50 mL of the solution and mix it with 200 mL of a 70 mmol / L K2S2O8 aqueous solution, place it away from light for 10 hours, and obtain an ABTS solution for use. Dilute the ABTS solution with a pH of 6.3 phosphate buffer to an absorbance of 1.0 to obtain a diluted ABTS solution; take 1 mL of the sample (the extract is prepared by diluting an 85% ethanol solution, and the extract concentration is 1.5%), add 2 mL of the diluted ABTS solution, mix; place it for 10 minutes; measure the absorbance of different solutions at 734 nm with a spectrophotometer. Calculate the ABTS clearance rate K = (A0-A) / A0×100%; where A0 is the absorbance of the diluted ABTS solution, and A is the absorbance of the mixture of 1 mL sample + 2 mL diluted ABTS solution.

[0083] The test results of the antioxidant effect of each extract dilution are shown in Table 1.

[0084] Table 1: Antioxidant effect test

[0085] Testing / 1.5% DPPH removal rate T / % ABTS clearance rate K / % Extract 1 77.91 65.48 Extract 2 76.53 64.41 Extract 3 70.68 59.92 Extract 4 75.21 63.40 Extract 5 73.49 62.07 Extract 6 69.87 59.26 Extract 7 66.34 56.54 Extract 8 74.93 63.19 Extract 9 73.06 61.75 Extract 10 64.12 54.86

[0086] According to the test, the extract prepared by the present invention has a good effect of resisting free radicals, giving the skin anti-aging and oxidation functions. After the extract is diluted to 1.5%, the scavenging rates for DPPH and ABTS free radicals reach 77.91% and 65.48%.

[0087] According to the test of extracts 1, 3-5, the present invention uses serrapeptase and snail protease for co-fermentation, which increases the types and richness of active ingredients in the extract, gives the extract a better free radical scavenging effect, and the two have a mutually reinforcing relationship in enhancing the free radical scavenging effect, and at the same time has a better effect than enzymatic hydrolysis with other proteases.

[0088] According to the test of extracts 1, 6-10, the extracts prepared by the specific fermentation bacteria Paenibacillus polymyxa CICC23712 and Lactobacillus reuteri CICC6126 in the present invention have better free radical scavenging effect than the extracts obtained by other fermentation bacteria, and can give the cosmetic composition excellent anti-aging function. The two have a synergistic relationship in enhancing the free radical scavenging effect, and when the ratio is 1:0.2-0.8, the obtained extract has a better effect.

[0089] B. Moisturizing effect test

[0090] 2. Moisturizing experiment

[0091] Volunteers aged 30-45 with healthy skin and no ethanol allergy were recruited and randomly divided into 11 groups according to age, with 4 males and 4 females in each group. A 2.5cm×2.5cm area of ​​the volunteer's right arm was taken as the test area, and the sample was used in the test area (the above extract was diluted to a mass concentration of 2.5% with ethanol), and the usage amount was 0.5g / person. The moisture content of the test area was tested before using the sample, 3 hours and 10 hours after using the composition (five points were randomly selected for each test and the average value was taken), the environment was 25°C and the humidity was 55%; the increase in the skin moisture content of the test area after using the composition for 3 hours and 10 hours was calculated (compared to the increase before using the sample). The results are shown in Table 5.

[0092] Table 5: Moisturizing test

[0093]

[0094]

[0095] According to the test, the extract prepared by the present invention has a good moisturizing effect on the skin and can effectively give the lips a plump and hydrated state. Extract 1-2 has a better moisturizing effect than Extract 3, indicating that the specific enzyme combination of the present invention has a better effect than other enzymes. Extract 1 adopts the combined enzymatic hydrolysis process of the present invention compared to Extracts 4-5, giving the extract-promoting composition a better moisturizing effect. Composition 1 adopts the process of co-fermentation of the specific fermentation bacteria of the present invention compared to Compositions 6-10, so that the moisturizing effect of the plant-promoting composition shows significant improvement.

[0096] 2. Preparation and testing of the composition

[0097] The composition and dosage (parts by weight) of the composition are shown in Table 2.

[0098] Table 2: Composition and dosage

[0099]

[0100]

[0101] Among them, the extract used in composition 1 is the above-mentioned extract 1; the extract used in composition 2 is the above-mentioned extract 2.

[0102] Compared with composition 1, composition 4-11 uses extracts 3-10 to replace extract 1 in sequence, and the rest are the same.

[0103] The preparation method of the above composition is as follows:

[0104] Step 1: heat and melt oils (caprylic / capric triglyceride, glyceryl trimyristate, avocado oil, camellia oil, coconut oil) at 85° C., add polyols (ethylene glycol, glycerol, 1,3-butylene glycol), stir and mix at 60 rpm for 0.3 hours to obtain a mixture 1;

[0105] Step 2: Cooling the mixture 1 to 42° C., adding the fermented plant extract, stirring at 65 rpm for 0.3 hours to obtain a mixture 2;

[0106] Step 3: Cool the mixture 2 to 30° C., add recombinant collagen, stir at 60 rpm for 0.4 hours to mix, sterilize, and package to obtain the oil-soluble lip care composition for repairing the barrier containing multiple recombinant collagens.

[0107] 1. Human patch test

[0108] The above composition was diluted to a concentration of 100 mg / mL with a PBS buffer solution of pH 7 and tested as a sample. Volunteers aged between 25 and 45 were selected as test subjects and randomly divided into groups of 12 people in each group. 0.05 mL of the sample was taken with a syringe and placed in a spot tester, and then the spot test tape was attached to the skin of the volunteer's left forearm, and the air was expelled by light pressure to make the sample evenly distributed. After 24 hours, the observation was carried out, and the results of the skin irritation test were evaluated according to the skin irritation test standards recorded in Table 3. The test results are shown in Table 4.

[0109] Table 3: Skin irritation test standards

[0110] grade Degree of reaction Skin reactions 0 - Negative: no irritation, no erythema 1 ± Weakly positive: slight erythema 2 + Weak positive: erythema 3 ++ Strong positive: erythema, papules, blisters 4 +++ Very strong positive: severe edema, large bullae

[0111] Table 4 Skin irritation test results

[0112]

[0113]

[0114] According to the test in Table 5, the composition prepared by the present invention has low irritation and high safety.

[0115] 2. Smear test

[0116] Ten volunteers aged 35-45 were recruited, 5 males and 5 females. All ten volunteers used composition 1-2 on their lips and scored them. The evaluation indexes included moisturizing effect (maximum 5 points, minimum 1 point), delicate effect (maximum 5 points, minimum 1 point) and moisturizing effect (maximum 5 points, minimum 1 point); each test item could be scored in decimals. The test results are shown in Table 6.

[0117] Table 6: Smear test

[0118] test Moisturizing score Detailed rating Moisturizing score Composition 1 4.7 4.6 4.8 Composition 2 4.6 4.7 4.7

[0119] According to the test results, the composition prepared by the present invention has a good effect of moisturizing and hydrating the lips, and has a relatively delicate effect. The composition of the present invention has an excellent use experience and has a good application prospect.

[0120] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. An oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens, characterized in that: The raw materials are as follows, in parts by mass: 0.01-0.5 parts of recombinant collagen, 0.05-1 parts of fermented plant extract, 20-40 parts of oil and fat, and 10-20 parts of polyol.

2. The oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens according to claim 1, characterized in that: The recombinant collagen includes recombinant collagen type I, type III and type XVII, and the mass ratio of the recombinant collagen type I, type III and type XVII is 1:(0.5-1.2):(0.5-1.2).

3. The oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens according to claim 1, characterized in that: Oils and fats include synthetic esters and vegetable oils.

4. The oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens according to claim 1, characterized in that: The polyol includes at least one of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, butylene glycol, and sorbitol.

5. The oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens according to claim 1, characterized in that: The raw materials for preparing the fermented plant extract include at least one of qinjiao, xuduan, caishen leaves and crab shell.

6. The oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens according to claim 1, characterized in that: The synthetic ester includes at least one of caprylic / capric triglyceride, cetyl lactylate, myristyl lactylate, trimyristinyl ester, castor oil fatty acid methyl ester, and oleic acid glyceryl.

7. The oil-soluble lip care composition for repairing the lip barrier containing multiple recombinant collagens according to claim 1, characterized in that: The vegetable oil includes at least one of avocado oil, camellia oil, jojoba oil, soybean oil, palm oil, almond oil, and coconut oil.

8. A method for preparing an oil-soluble lip care composition for repairing the barrier containing multiple recombinant collagens according to claims 1-7, characterized in that: The steps include: Step 1: Heat and melt the oil, add the polyol, and stir to mix to obtain a mixture 1; Step 2: Cooling the mixture 1, adding the fermented plant extract, stirring and mixing, to obtain a mixture 2; Step 3: Cool the mixture 2, add recombinant collagen, and stir to mix, so as to obtain the barrier-repairing oil-soluble lip care composition containing multiple recombinant collagens.

9. The preparation method according to claim 8, characterized in that: The heating melting temperature in step 1 is 80-95°C.

10. The preparation method according to claim 8, characterized in that: In step 2, the temperature is lowered to 40-55°C, and in step 3, the temperature is lowered to 25-35°C.

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