Composite hyaluronic acid solution capable of dehydrating and decomposing fat

Abstract

The invention discloses a composite hyaluronic acid solution capable of dehydrating and decomposing fat, and relates to the technical field of biomedical materials. Adding the sodium hyaluronate into ultrapure water, and standing for 5-6 hours until the sodium hyaluronate is completely hydrated to prepare pure gel; placing the pure gel in a water bath kettle, gently stirring and heating, adding normal saline, homogenizing for 3 minutes, adding a composite vitamin B injection, uniformly stirring, gently stirring, and preparing a composite liquid; and sterilizing the composite liquid, and sub-packaging the sterilized composite liquid into sterile bottles to prepare the composite hyaluronic acid solution capable of dehydrating and decomposing fat. The face-lift mask is free of side effects, suitable for high-sensitivity people, good in face-lift effect and fast in face-lift recovery.

Description

Technical Field

[0001] The invention relates to the technical field of biomedical materials, in particular to a composite hyaluronic acid solution capable of dehydrating and decomposing fat. Background Art

[0002] Common face-slimming products and projects on the market mainly include type A bacilli toxin (botulinum toxin) face-slimming injections and liposuction surgery. Among them, face-slimming injections with botulinum toxin as the main active ingredient have certain complications during use, and botulinum toxin injections are mainly used to target masseter muscle hypertrophy, and cannot be used as a face-slimming method for fat-prone areas. Even if injected into the masseter muscle, it is easy to affect facial expressions or cause facial stiffness, and there is also a risk of toxic reactions, and it is not suitable for highly sensitive people; the professional term for facial liposuction surgery is tumescent anesthesia negative pressure liposuction, which has greater risks and more side effects, including but not limited to: infection, uneven surface, local tissue adhesion and hardening, and the risk of fat embolism. Medical beauty consumers are in urgent need of a product that has no side effects, is suitable for highly sensitive people, has good fat-slimming effects, and has a quick recovery.

[0003] Sodium hyaluronate is an inherent component of the human body and an anionic glycosaminoglycan. It is widely present in tissues such as the placenta, amniotic fluid, lens, articular cartilage, and skin dermis. It has good hydrophilicity, water retention, biocompatibility, and non-immunogenicity. It has been widely used in plastic surgery, tissue engineering, osteoarthritis relief, and lubrication. Summary of the invention

[0004] The object of the present invention is to provide a composite hyaluronic acid solution capable of dehydrating and decomposing fat, so as to solve the problems raised in the background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A composite hyaluronic acid solution capable of dehydrating and decomposing fat, the specific steps of the preparation process are as follows:

[0007] S1. Add sodium hyaluronate to ultrapure water and let stand for 5-6 hours until completely hydrated to obtain a simple gel;

[0008] It should be noted that the simple gel is a non-cross-linked hyaluronic acid gel.

[0009] S2. Place the simple gel in a water bath, stir gently and heat, add normal saline, homogenize for 3 min, add vitamin B complex injection, stir evenly, stir gently to make a composite solution;

[0010] S3. Sterilize the composite liquid and dispense it into sterile bottles to obtain a composite hyaluronic acid solution that can dehydrate and decompose fat.

[0011] Furthermore, the molecular weight of the sodium hyaluronate described in S1 is 1.2 million.

[0012] Furthermore, the dosage ratio of sodium hyaluronate to ultrapure water in S1 is 1 g:30 mL.

[0013] Furthermore, the volume ratio of the simple gel, normal saline and vitamin B complex injection described in S2 is 90:10:1.

[0014] Furthermore, the heating in S2 is heating to 50°C.

[0015] Furthermore, the homogenization condition in S2 is continuous stirring at a rotation speed of 30-45 r / min.

[0016] Furthermore, the vitamin B complex injection described in S2 contains vitamin B1, vitamin B2, vitamin B3, vitamin B5 and vitamin B6.

[0017] It should be noted that vitamin B complex participates in the body's metabolism, providing essential coenzymes for various metabolic links in the body and important coenzymes for tissue respiration; vitamin B1 is an important component of coenzymes required for carbohydrate metabolism; vitamin B2 is an important component of coenzymes required for tissue respiration; vitamin B6 is a cofactor for many enzymes and participates in the metabolism of amino acids and fats; vitamin B3 niacin is a component of coenzymes I and II, which is necessary for lipid metabolism, oxidation of tissue respiration and glycogenolysis. Niacin and niacinamide can be converted into each other in the human body, and niacinamide has the effect of whitening and removing spots; vitamin B5 pantothenic acid is a precursor of coenzyme A and participates in the metabolism of carbohydrates, fats and proteins. Semi-dehydrated fat will be metabolized out of the body with the metabolism of tissues, promoting the firmness and elasticity of skin tissues.

[0018] Furthermore, the sterilization treatment in S3 is ultraviolet sterilization.

[0019] Furthermore, the capacity of the sterile bottle in S3 is 10 mL.

[0020] Furthermore, the method of use is: using an ultra-fine needle, the composite hyaluronic acid solution that can dehydrate and decompose fat is injected into the subcutaneous fat layer in layers under pressure.

[0021] It should be noted that non-cross-linked hyaluronic acid has strong water absorption. Using a small amount can increase skin moisture and maintain skin humidity. When the prepared hyaluronic acid gel is injected into the subcutaneous fat layer in large quantities, it will quickly absorb moisture from the surrounding tissues, causing the surrounding fat tissues to be temporarily semi-dehydrated, thereby reducing the volume of the surrounding tissues. The semi-dehydrated fat will be metabolized out of the body as the tissues metabolize and local swelling subsides, and the skin tissue will then become firm and elastic, avoiding excess skin, orange peel, and wrinkled skin.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. Compared with most commercially available face-slimming injections, the present invention has simple ingredients, no side effects, is suitable for highly sensitive people with non-endocrine disorders, has excellent fat decomposition effect, and has a simple preparation process.

[0024] 2. Compared with the common product of hyaluronic acid solution: the cross-linked hyaluronic acid gel filler used for facial filling, the present invention adopts a simple gel made of non-cross-linked hyaluronic acid. On the one hand, it can make the adipose tissue semi-dehydrated and reduce its volume, which is contrary to the effect of cross-linked hyaluronic acid gel in promoting stable fat production. On the other hand, the ingredients of the present invention are only composed of components contained in the human body and important vitamins for maintaining normal functions and metabolic activities of the human body. The ingredients are simple and healthy, while cross-linked hyaluronic acid is generally cross-linked with some polymer materials that are not contained in the human body itself, which has a certain degree of toxicity.

[0025] 3. Compared with traditional liposuction surgery, the composite hyaluronic acid solution prepared by the present invention is injected into the subcutaneous fat layer with an ultra-fine needle, which causes only slight and negligible pain, no cyanosis, no unevenness, no hardening, no loosening and wrinkling of the skin, and can be recovered within 48 hours. It is suitable for people with more facial fat accumulation, can make the facial lines smoother, and the fat is naturally discharged with metabolism, reducing local fat accumulation on the face. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is an ultrasonic diagnostic image of the mandibular fat on the face of a test subject before injection of the composite hyaluronic acid solution prepared in Example 1.

[0027] In the picture: Fat is obviously accumulated at the jawline.

[0028] Figure 2 This is an ultrasonic diagnostic image of the mandibular fat on the face of a subject 20 days after injection of the composite hyaluronic acid solution prepared in Example 1.

[0029] In the picture: The fat at the jawline is significantly thinner than that at Figure 1 70.5% reduction in fat. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] A composite hyaluronic acid solution capable of dehydrating and decomposing fat, the preparation process of which is as follows:

[0033] S1. Add 3 g of sodium hyaluronate with a molecular weight of 1.2 million to 90 mL of ultrapure water and let stand for 5-6 hours until completely hydrated to prepare a simple gel;

[0034] S2. Place 90 mL of simple gel in a water bath, stir gently and heat to 50°C, add 10 mL of normal saline, homogenize at 30 r / min for 3 min, add 1 mL of complex vitamin B injection containing vitamin B1, vitamin B2, vitamin B3, vitamin B5 and vitamin B6, stir evenly, stir gently to make a complex solution;

[0035] S3. The composite liquid is sterilized by ultraviolet light and dispensed into 10 mL sterile bottles to obtain a composite hyaluronic acid solution that can dehydrate and decompose fat.

[0036] Example 2

[0037] A composite hyaluronic acid solution capable of dehydrating and decomposing fat, the preparation process of which is as follows:

[0038] S1. Add 3 g of sodium hyaluronate with a molecular weight of 1.2 million to 90 mL of ultrapure water and let stand for 5-6 hours until completely hydrated to prepare a simple gel;

[0039] S2. Place 90 mL of simple gel in a water bath, stir gently and heat to 50°C, add 10 mL of normal saline, homogenize at 45 r / min for 3 min, add 1 mL of complex vitamin B injection containing vitamin B1, vitamin B2, vitamin B3, vitamin B5 and vitamin B6, stir evenly, stir gently to make a complex solution;

[0040] S3. The composite liquid is sterilized by ultraviolet light and dispensed into 10 mL sterile bottles to obtain a composite hyaluronic acid solution that can dehydrate and decompose fat.

[0041] Comparative Example 1

[0042] A composite hyaluronic acid solution capable of dehydrating and decomposing fat, the preparation process of which is as follows:

[0043] S1. Add 3 g of sodium hyaluronate with a molecular weight of 1.2 million to 90 mL of ultrapure water and let stand for 5-6 hours until completely hydrated to prepare a simple gel;

[0044] S2. Place 90 mL of simple gel in a water bath, stir gently and heat to 50°C, add 10 mL of normal saline, and homogenize at 40 r / min for 3 min to prepare a composite solution;

[0045] S3. The composite liquid is sterilized by ultraviolet light and dispensed into 10 mL sterile bottles to obtain a composite hyaluronic acid solution that can dehydrate and decompose fat.

[0046] The difference between this comparative example and Example 1 is that no vitamin B complex injection is added.

[0047] Comparative Example 2

[0048] A composite hyaluronic acid solution capable of dehydrating and decomposing fat, the preparation process of which is as follows:

[0049] S1. Place 90 mL of cross-linked hyaluronic acid gel in a water bath, gently stir and heat to 50°C, add 10 mL of normal saline, and homogenize at 40 r / min for 3 min to prepare a composite solution;

[0050] S3. The composite liquid is sterilized by ultraviolet light and dispensed into 10 mL sterile bottles to obtain a composite hyaluronic acid solution that can dehydrate and decompose fat.

[0051] The cross-linked hyaluronic acid gel is an HA filler produced by Shandong Pangshi Biotechnology Co., Ltd.

[0052] The difference between this comparative example and Example 1 is that a commercially available cross-linked hyaluronic acid gel is used instead of a non-cross-linked simple gel.

[0053] test:

[0054] 1. Human Evaluation Method

[0055] (1) Inclusion criteria:

[0056] ①BMI>24kg / m 2 , there is a lot of fat accumulation on the face, especially under the jaw;

[0057] ② The subjects were aged between 25 and 50 years old, regardless of gender;

[0058] ③ The subjects are willing to cooperate with the test requirements during the entire research test period and not use other products of the same type at the same time;

[0059] ④ Eat normally during the test period.

[0060] (2) Exclusion criteria:

[0061] ① Women who are pregnant, within six months after childbirth, breastfeeding or preparing for pregnancy;

[0062] ② Patients with endocrine disorders represented by obesity.

[0063] ③ Have received or are receiving medication for endocrine diseases or systemic diseases within the past 3 months;

[0064] Physical therapy;

[0065] ④ Those who have received facial cosmetic surgery in the past 6 months;

[0066] ⑤ Those who are considered unsuitable to participate in the trial by the researchers.

[0067] (3) Test conditions: 40 test subjects were injected with the composite hyaluronic acid solutions prepared in Example 1-2 and Comparative Example 1-2 on their faces, with 5 ml injected into the bilateral corners of the mouth, 8 ml injected into each side of the mandibular margin, and 10 ml injected into the chin. One test subject was injected with only one composite hyaluronic acid solution. There were 10 test subjects in each of Example 1-2 and Comparative Example 1-2. Ultrasonic diagnosis was performed on the test subjects' faces and mandibles before injection, 5 days after injection, and 20 days after injection to observe the fat decomposition. The evaluation results of the residual fat content were averaged within each group.

[0068] 2. Human Evaluation Results

[0069] As shown in Table 1.

[0070]

[0071] Table 1

[0072] 3. Conclusion

[0073] As shown in Table 1, the fat decomposition effect of Example 1-2 is significantly better than that of Comparative Example 1-2, and can decompose and metabolize up to 70% of the fat in the mandible; the fat decomposition effect of Comparative Example 1 is poor because no complex vitamin B solution that can promote fat decomposition and body metabolism is added; and Comparative Example 2 not only fails to decompose fat, but further promotes fat generation. This is because the cross-linked sodium hyaluronate gel is used.

[0074] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0075] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A composite hyaluronic acid solution capable of dehydrating and decomposing fat, characterized in that: The specific steps of the preparation process are as follows: S1. Add sodium hyaluronate to ultrapure water and let stand for 5-6 hours until completely hydrated to obtain a simple gel; S2. Place the simple gel in a water bath, gently stir and heat, add normal saline, homogenize for 3 minutes, add vitamin B complex injection, stir evenly, and prepare a complex solution; S3. Sterilize the composite liquid and dispense it into sterile bottles to obtain a composite hyaluronic acid solution that can dehydrate and decompose fat.

2. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The molecular weight of sodium hyaluronate described in S1 is 1.2 million.

3. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The dosage ratio of sodium hyaluronate to ultrapure water in S1 is 1 g:30 mL.

4. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The volume ratio of the simple gel, normal saline and vitamin B complex injection described in S2 is 90:10:

1.

5. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The heating in S2 is to raise the temperature to 50°C.

6. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The homogenization condition in S2 is continuous stirring at a rotation speed of 30-45 r / min.

7. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The vitamin B complex injection described in S2 contains vitamin B1, vitamin B2, vitamin B3, vitamin B5 and vitamin B6.

8. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The sterilization treatment described in S3 is ultraviolet sterilization.

9. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The capacity of the sterile bottle S3 is 10 mL.

10. The composite hyaluronic acid solution capable of dehydrating and decomposing fat according to claim 1, characterized in that: The method of use is: use an ultra-fine needle to inject the composite hyaluronic acid solution that can dehydrate and decompose fat into the subcutaneous fat layer in layers under pressure.

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