Traditional Chinese medicine composition for gynecological diseases and preparation method thereof

Abstract

The invention provides a traditional Chinese medicine composition for gynecological diseases. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 50-150 parts of fructus cnidii, 20-50 parts of herba schizonepetae, 50-150 parts of cortex dictamni, 20-50 parts of radix saposhnikoviae, 50-150 parts of cortex phellodendri, 40-80 parts of radix sophorae flavescentis, 20-50 parts of radix gentianae, 5-20 parts of pericarpium zanthoxyli, 10-20 parts of radix stemonae, 10-30 parts of cockscomb and 20-50 parts of herba taraxaci. The traditional Chinese medicine composition provided by the invention has the effects of clearing heat, drying dampness, killing parasites and relieving itching, is suitable for treating female vulva eczema caused by wind-heat damp toxin, and has good effects on symptoms such as flushing, itching, swelling and erosion of female private parts and surrounding skin.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for gynecological diseases and a preparation method thereof. Background Art

[0002] Gynecological diseases are one of the main causes that affect women's health. Common gynecological diseases in modern medicine include vaginitis, cervicitis, pelvic inflammatory disease, etc., which are collectively referred to as leukorrhea in traditional Chinese medicine. The name "leukorrhea" was first seen in the "Inner Canon of Medicine". As far as gynecological diseases are concerned, since these diseases all occur under the belt meridian, they are called "leukorrhea". The main cause of leukorrhea is dampness. "Fu Qingzhu's Gynecology" says: "Leukorrhea is a symptom of dampness." "Suwen Taiyin Yangming Lun" records: "Those who are injured by dampness will be affected first." The most common manifestation of these diseases in clinical practice is vulvar eczema, which is a gynecological disease with redness, itching, swelling, erosion, etc. of the labia majora and minora and the skin around the anus.

[0003] In modern medicine, antibiotics such as metronidazole, fluconazole, and miconazole nitrate are often used to treat vulvar eczema. These drugs have a more significant effect in the initial use, but long-term use of these antibiotics will cause the pathogenic strains to develop drug resistance, which in turn leads to a decrease in the antibacterial effect, resulting in repeated attacks of the disease. In traditional Chinese medicine, the treatment of leucorrhea is based on the principles of clearing heat, detoxifying, and removing dampness. Commonly used prescriptions include Wandai Decoction and Wuwei Xiaodu Yin. The main ingredients of Wandai Decoction are yam, ginseng, plantain seeds, black mustard spikes, etc. The whole prescription has the functions of strengthening the spleen and replenishing qi, raising yang and removing dampness; Wuwei Xiaodu Yin contains dandelion, honeysuckle, wild chrysanthemum, Viola yedoensis, and Tiankui seeds, and the whole prescription has the functions of clearing heat, detoxifying, and removing dampness. However, these traditional prescriptions often have a long use period and a slow onset of effect. Summary of the invention

[0004] The invention provides a Chinese medicine composition for gynecological diseases and a preparation method thereof, which are used to solve the problem that the Chinese medicine composition in the prior art has a poor effect in treating vulvar eczema.

[0005] The present invention provides a traditional Chinese medicine composition for gynecological diseases, which is composed of the following raw materials in proportions by weight:

[0006] 50-150 parts of Fructus Cnidium, 20-50 parts of Herba Schizonepetae, 50-150 parts of Cortex Dictamni, 20-50 parts of Saposhnikoviae, 50-150 parts of Cortex Phellodendri, 40-80 parts of Sophora flavescens, 20-50 parts of Gentiana, 5-20 parts of Zanthoxylum bungeanum, 10-20 parts of Radix Stemonae, 10-30 parts of Flos Celosiae, and 20-50 parts of Herba Taraxaci.

[0007] Optionally, the Chinese medicine composition is composed of the following raw materials in the following weight proportions: 70-120 parts of Cnidium monnieri, 30-40 parts of Schizonepeta tenuifolia, 70-120 parts of Dictamni cortex, 30-40 parts of Saposhnikovia divaricata, 70-120 parts of Phellodendron chinense, 50-70 parts of Sophora flavescens, 30-40 parts of Gentiana scabra, 5-10 parts of Zanthoxylum bungeanum, 10-20 parts of Stemona japonica, 10-30 parts of Celosia cristata, and 20-30 parts of Taraxacum officinale.

[0008] Optionally, the Chinese medicine composition is composed of the following raw materials in the following weight proportions: 100 parts of Cnidium monnieri, 30 parts of Schizonepeta tenuifolia, 100 parts of Dictamni cortex, 30 parts of Saposhnikovia divaricata, 100 parts of Phellodendron chinense, 60 parts of Sophora flavescens, 30 parts of Gentiana scabra, 5 parts of Zanthoxylum bungeanum, 10 parts of Stemona bulbs, 10 parts of Celosia cristata, and 20 parts of Taraxacum officinale.

[0009] Optionally, the dosage form of the Chinese medicine composition is one of lotion, ointment and spray.

[0010] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition, comprising the following steps:

[0011] (1) Weigh the following raw materials in parts by weight: 50-150 parts of Cnidium monnieri, 20-50 parts of Schizonepeta tenuifolia, 50-150 parts of Dictamni cortex, 20-50 parts of Saposhnikovia divaricata, 50-150 parts of Phellodendron chinense, 40-80 parts of Sophora flavescens, 20-50 parts of Gentiana scabra, 5-20 parts of Zanthoxylum bungeanum, 10-20 parts of Stemona tuberosum, 10-30 parts of Celosia cristata, and 20-50 parts of Taraxacum officinale;

[0012] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0013] (3) mixing the volatile oil A and the aqueous solution B, adding polysorbate 80 thereto, and mixing well to obtain a clear drug solution D;

[0014] (4) boiling the residue C with Dictamni cortex, Phellodendron amurense, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata with water, and filtering the mixture to obtain a concentrated solution E;

[0015] (5) adding ethanol to the concentrated solution E and allowing the solution to stand, and then filtering the solution to obtain an alcohol precipitate F;

[0016] (6) boiling the stemonae and dandelions in an ethanol solution and filtering the resulting mixture; boiling the residue in an ethanol solution again and filtering the resulting mixture; combining the two filtrates to obtain an ethanol extract G;

[0017] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, adding activated carbon to the solution after ethanol recovery for adsorption, and then filtering to obtain a concentrated solution H and a concentrated solution I, respectively;

[0018] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and a preservative is added to obtain a Chinese medicine composition.

[0019] Optionally, the ethanol concentration in step (5) is 65%, and the ethanol concentration in step (6) is 75%.

[0020] Optionally, in step (4), water is added and decocted for three times, the first time for two hours, the second time for 1.5 hours, and the third time for 1 hour, and the medicinal liquids are combined after decocting.

[0021] Optionally, the relative density of the concentrated liquid E is 1.10-1.20.

[0022] Optionally, the Chinese medicine composition needs to be mixed with clean water in a ratio of 1:10 before use.

[0023] The medicinal properties of the Chinese medicine composition provided by the present invention are as follows: Cnidium monnieri: pungent, bitter, warm; it enters the kidney meridian and has the functions of drying dampness and dispelling wind, killing insects and relieving itching.

[0024] Schizonepeta: pungent, slightly warm. Enters the lung and liver meridians. Has the effects of dispelling wind, clearing rashes and eliminating sores.

[0025] Dictamni: bitter, cold. Enters the spleen, stomach, and bladder meridians. Has the effects of clearing away heat and dampness, dispelling wind and detoxifying

[0026] Saposhnikovia root: pungent, sweet, slightly warm. Enters the bladder, liver, and spleen meridians. Expels wind and relieves exterior symptoms, overcomes dampness and relieves pain, and stops spasms.

[0027] Phellodendron chinense: bitter, cold. Enters the kidney and bladder meridians. Clears away heat and dampness, purges fire and eliminates steam, detoxifies and treats sores.

[0028] Sophora flavescens: bitter, cold. Enters the heart, liver, stomach, large intestine, and bladder meridians. Has the effects of clearing away heat and dampness, purging fire and detoxifying.

[0029] Gentian: bitter, cold. Enters the liver and gallbladder meridians. Clears away heat and dampness, and relieves liver and gallbladder fire.

[0030] Zanthoxylum bungeanum: spicy, warm. Enters the spleen, stomach, and kidney meridians. Warms the middle and relieves pain, kills insects and relieves itching.

[0031] Radix Stemonae: sweet, bitter, slightly warm. Enters the lung meridian. Kills insects and lice.

[0032] Celosia: sweet, astringent, cool. Enters the liver and large intestine meridians. Astringent, hemostatic, leucorrhea, and dysentery.

[0033] Dandelion: bitter, sweet, cold. Enters the liver and stomach meridians. Clears away heat and detoxifies, reduces swelling and resolves stagnation, and promotes diuresis and relieves stranguria.

[0034] The Chinese medicine composition provided by the present invention has the effects of clearing away heat and drying dampness, killing insects and relieving itching, is suitable for female vulva eczema caused by wind-heat-dampness toxins, and has good effects on the symptoms of flushing, itching, swelling, erosion and the like of female genitals and surrounding skin. Among them, Zanthoxylum bungeanum, Radix Stemonae and Fructus Cnidii are pungent and warm, and the combination of the three can restrict the coldness of the other medicines, avoid excessive coldness and damage to Yang Qi, and the combination of the three can increase the effect of killing insects and relieving itching. Celosia cristata has significant effects of stopping bleeding and stopping leucorrhea, relieving pain and improving body immunity. Taraxacum officinale clears away heat and detoxifies, and relieves swelling and disperses nodules. At the same time, when preparing the Chinese medicine composition, activated carbon adsorption is added to the solution after recovering ethanol, and the impurities in the solution can be further reduced by the adsorption of activated carbon, thereby improving product quality. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work also fall within the scope of protection of the present invention.

[0036] The present invention provides a traditional Chinese medicine composition for gynecological diseases, which is composed of the following raw materials in proportions by weight:

[0037] 50-150 parts of Fructus Cnidium, 20-50 parts of Herba Schizonepetae, 50-150 parts of Cortex Dictamni, 20-50 parts of Saposhnikoviae, 50-150 parts of Cortex Phellodendri, 40-80 parts of Sophora flavescens, 20-50 parts of Gentiana, 5-20 parts of Zanthoxylum bungeanum, 10-20 parts of Stemona, 10-30 parts of Celosia cristata, and 20-50 parts of Taraxacum.

[0038] Furthermore, the Chinese medicine composition is composed of the following raw materials in proportion by weight: 70-120 parts of Cnidium monnieri, 30-40 parts of Schizonepeta tenuifolia, 70-120 parts of Dictamni cortex, 30-40 parts of Saposhnikovia divaricata, 70-120 parts of Phellodendron chinense, 50-70 parts of Sophora flavescens, 30-40 parts of Gentiana scabra, 5-10 parts of Zanthoxylum bungeanum, 10-20 parts of Stemona japonica, 10-30 parts of Celosia cristata, and 20-30 parts of Taraxacum officinale.

[0039] Furthermore, the Chinese medicine composition is composed of the following raw materials in the following weight proportions: 100 parts of Cnidium monnieri, 30 parts of Schizonepeta tenuifolia, 100 parts of Dictamni cortex, 30 parts of Saposhnikovia divaricata, 100 parts of Phellodendron chinense, 60 parts of Sophora flavescens, 30 parts of Gentiana scabra, 5 parts of Zanthoxylum bungeanum, 10 parts of Stemona bulbs, 10 parts of Celosia cristata, and 20 parts of Taraxacum officinale.

[0040] Furthermore, the dosage form of the Chinese medicine composition is one of lotion, liniment and spray.

[0041] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition, comprising the following steps:

[0042] (1) Weigh the following raw materials in parts by weight: 50-150 parts of Cnidium monnieri, 20-50 parts of Schizonepeta tenuifolia, 50-150 parts of Dictamni cortex, 20-50 parts of Saposhnikovia divaricata, 50-150 parts of Phellodendron chinense, 40-80 parts of Sophora flavescens, 20-50 parts of Gentiana scabra, 5-20 parts of Zanthoxylum bungeanum, 10-20 parts of Stemona officinalis, 10-30 parts of Celosia cristata, and 20-50 parts of Taraxacum officinale;

[0043] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0044] (3) mixing the volatile oil A and the aqueous solution B, adding polysorbate 80 thereto, and mixing well to obtain a clear drug solution D;

[0045] (4) boiling the residue C with Dictamni cortex, Phellodendron amurense, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata with water, and filtering the mixture to obtain a concentrated solution E;

[0046] (5) adding ethanol to the concentrated solution E and allowing the solution to stand, and then filtering the solution to obtain an alcohol precipitate F;

[0047] (6) boiling the stemonae and dandelions in an ethanol solution and filtering the resulting mixture; boiling the residue in an ethanol solution again and filtering the resulting mixture; combining the two filtrates to obtain an ethanol extract G;

[0048] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, adding activated carbon to the solution after ethanol recovery for adsorption, and then filtering to obtain a concentrated solution H and a concentrated solution I, respectively;

[0049] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and a preservative is added to obtain a Chinese medicine composition.

[0050] Furthermore, the ethanol concentration in step (5) is 65%, and the ethanol concentration in step (6) is 75%.

[0051] Furthermore, in step (4), water is added and decocted for three times, the first time for two hours, the second time for 1.5 hours, and the third time for one hour, and the medicinal liquids are combined after decocting.

[0052] Furthermore, the relative density of the concentrated solution E is 1.10-1.20.

[0053] Furthermore, the Chinese medicine composition needs to be mixed with clean water in a ratio of 1:10 before use.

[0054] The present invention is further described in detail below in conjunction with specific embodiments.

[0055] Example 1

[0056] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 50 parts of cnidium monnieri, 20 parts of schizonepeta tenuifolia, 50 parts of dittanyi cortex, 20 parts of siler, 50 parts of phellodendron amurense, 40 parts of sophora flavescens, 20 parts of gentian, 5 parts of zanthoxylum bungeanum, 10 parts of stemonae, 10 parts of cockscomb and 20 parts of dandelion (the above weight units are in grams).

[0057] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0058] (1) Weigh the raw materials according to the above components;

[0059] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0060] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0061] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0062] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0063] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 10 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 10 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0064] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding activated carbon with a concentration of 0.5% of the drug solution to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0065] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0066] Example 2

[0067] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 150 parts of cnidium monnieri, 50 parts of schizonepeta tenuifolia, 150 parts of dittanyi cortex, 50 parts of siler, 150 parts of phellodendron amurense, 80 parts of sophora flavescens, 50 parts of gentian, 20 parts of zanthoxylum bungeanum, 20 parts of stemona, 30 parts of cockscomb and 50 parts of dandelion (the above weight units are in grams).

[0068] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0069] (1) Weigh the raw materials according to the above components;

[0070] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0071] (3) Mix the volatile oil A and the aqueous solution B, add 1200 ml of polysorbate 80 thereto, and mix well to obtain a clear solution D;

[0072] (4) adding water 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata to decoct for 2 hours, filtering; adding water 6 times the weight of the medicinal materials again to decoct for 1.5 hours, filtering; adding water 6 times the weight of the medicinal materials to decoct for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0073] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0074] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 12 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 12 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0075] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding activated carbon with a concentration of 0.5% of the drug solution to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0076] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0077] Example 3

[0078] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 100 parts of cnidium monnieri, 40 parts of schizonepeta tenuifolia, 100 parts of dittanyi root, 40 parts of siler, 100 parts of phellodendron amurense, 60 parts of sophora flavescens, 30 parts of gentian, 15 parts of zanthoxylum bungeanum, 15 parts of stemona, 15 parts of cockscomb and 40 parts of dandelion (the above weight units are in grams).

[0079] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0080] (1) Weigh the raw materials according to the above components;

[0081] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0082] (3) Mix the volatile oil A and the aqueous solution B, add 1100 ml of polysorbate 80 thereto, and mix well to obtain a clear solution D;

[0083] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0084] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0085] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 11 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decoct the residue again in an ethanol solution 11 times the amount of the medicinal materials, and decocting and filtering, and combining the two filtrates to obtain an alcohol extract G;

[0086] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding activated carbon with a concentration of 0.5% of the drug solution to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0087] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0088] Example 4

[0089] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 90 parts of Cnidium monnieri, 30 parts of Schizonepeta tenuifolia, 90 parts of Dictamni cortex, 30 parts of Saposhnikovia divaricata, 90 parts of Phellodendron chinense, 50 parts of Sophora flavescens, 40 parts of Gentiana scabra, 10 parts of Zanthoxylum bungeanum, 15 parts of Stemona tuberosum, 15 parts of Celosia cristata and 30 parts of Taraxacum officinale (the above weight units are in grams).

[0090] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0091] (1) Weigh the raw materials according to the above components;

[0092] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0093] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0094] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0095] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0096] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 10 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 10 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0097] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding 0.5% of the drug solution amount of activated carbon to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0098] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0099] Example 5

[0100] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 100 parts of cnidium monnieri, 30 parts of schizonepeta tenuifolia, 100 parts of dittanyi root, 30 parts of siler, 100 parts of phellodendron, 60 parts of sophora flavescens, 30 parts of gentian, 5 parts of zanthoxylum bungeanum, 10 parts of stemonae, 10 parts of cockscomb and 20 parts of dandelion (the above weight units are in grams).

[0101] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0102] (1) Weigh the raw materials according to the above components;

[0103] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0104] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0105] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0106] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0107] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 9 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 9 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0108] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding activated carbon with a concentration of 0.5% of the drug solution to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0109] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0110] Example 6

[0111] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 110 parts of cnidium monnieri, 30 parts of schizonepeta tenuifolia, 110 parts of dittanyi cortex, 30 parts of siler, 110 parts of phellodendron amurense, 70 parts of sophora flavescens, 20 parts of gentian, 5 parts of zanthoxylum bungeanum, 15 parts of stemonae, 20 parts of cockscomb and 25 parts of dandelion (the above weight units are in grams).

[0112] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0113] (1) Weigh the raw materials according to the above components;

[0114] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0115] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0116] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0117] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0118] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 9 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 9 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0119] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding 0.5% of the drug solution amount of activated carbon to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0120] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0121] Example 7

[0122] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 130 parts of Cnidium monnieri, 30 parts of Schizonepeta tenuifolia, 130 parts of Dictamni cortex, 30 parts of Saposhnikovia divaricata, 130 parts of Phellodendron chinense, 60 parts of Sophora flavescens, 20 parts of Gentiana scabra, 5 parts of Zanthoxylum bungeanum, 10 parts of Stemona bulbs, 10 parts of Celosia cristata and 20 parts of Taraxacum officinale (the above weight units are in grams).

[0123] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0124] (1) Weigh the raw materials according to the above components;

[0125] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0126] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0127] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0128] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0129] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 9 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 9 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0130] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding 0.5% of the drug solution amount of activated carbon to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0131] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0132] Example 8

[0133] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 120 parts of Cnidium monnieri, 35 parts of Schizonepeta tenuifolia, 120 parts of Dictamni cortex, 35 parts of Saposhnikovia divaricata, 120 parts of Phellodendron chinense, 50 parts of Sophora flavescens, 25 parts of Gentiana scabra, 10 parts of Zanthoxylum bungeanum, 10 parts of Stemona tuberosum, 10 parts of Celosia cristata and 20 parts of Taraxacum officinale (the above weight units are in grams).

[0134] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0135] (1) Weigh the raw materials according to the above components;

[0136] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0137] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0138] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0139] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0140] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 9 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 9 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0141] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding activated carbon with a concentration of 0.5% of the drug solution to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0142] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0143] Example 9

[0144] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 80 parts of Cnidium monnieri, 40 parts of Schizonepeta tenuifolia, 120 parts of Dictamni cortex, 40 parts of Saposhnikovia divaricata, 80 parts of Phellodendron chinense, 80 parts of Sophora flavescens, 40 parts of Gentiana scabra, 15 parts of Zanthoxylum bungeanum, 20 parts of Stemona tuberosum, 25 parts of Celosia cristata and 45 parts of Taraxacum officinale (the above weight units are in grams).

[0145] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0146] (1) Weigh the raw materials according to the above components;

[0147] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0148] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0149] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0150] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0151] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 9 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 9 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0152] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding activated carbon with a concentration of 0.5% of the drug solution to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0153] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0154] Example 10

[0155] A traditional Chinese medicine composition for gynecological diseases comprises the following raw materials in proportion by weight: 100 parts of cnidium monnieri, 20 parts of schizonepeta tenuifolia, 100 parts of dittanyi root, 20 parts of siler, 100 parts of phellodendron, 75 parts of sophora flavescens, 35 parts of gentian, 10 parts of zanthoxylum bungeanum, 15 parts of stemona, 20 parts of cockscomb and 35 parts of dandelion (the above weight units are in grams).

[0156] The preparation process of the above-mentioned Chinese medicine composition is as follows:

[0157] (1) Weigh the raw materials according to the above components;

[0158] (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively;

[0159] (3) Mix the volatile oil A and the aqueous solution B, add 1000 ml of polysorbate 80 thereto, and mix well to obtain a clear drug solution D;

[0160] (4) adding 6 times the weight of the medicinal materials to the residue C and Dictamni, Phellodendron, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata, boiling for 2 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1.5 hours, filtering; adding 6 times the weight of the medicinal materials to boil for 1 hour, filtering; combining the three decoctions to obtain a concentrated solution E;

[0161] (5) adding ethanol to the concentrated solution E so that the alcohol content in the solution reaches 65%, allowing the solution to stand, and filtering after standing to obtain an alcohol precipitate F;

[0162] (6) decocting the Radix Stemonae and Herba Taraxaci in a 75% ethanol solution, wherein the amount of ethanol used is 9 times the total amount of the Radix Stemonae and Herba Taraxaci, filtering after decocting, decocting the residue again in an ethanol solution 9 times the amount of the medicinal materials, and filtering, combining the two filtrates to obtain an alcohol extract G;

[0163] (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, and adding activated carbon with a concentration of 0.5% of the drug solution to the solution after ethanol recovery for adsorption, followed by filtration to obtain a concentrated solution H and a concentrated solution I, respectively;

[0164] (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and sodium benzoate is added for preservation to obtain a Chinese medicine composition.

[0165] Comparative Example 1

[0166] The only difference between Comparative Example 1 and Example 1 is that no Zanthoxylum bungeanum is added to the raw materials of the Chinese medicine composition.

[0167] Comparative Example 2

[0168] The only difference between Comparative Example 2 and Example 2 is that Stemona bulbosa is not added to the raw materials of the Chinese medicine composition.

[0169] Comparative Example 3

[0170] The only difference between Comparative Example 3 and Example 3 is that no cockscomb is added to the raw materials of the Chinese medicine composition.

[0171] Comparative Example 4

[0172] The only difference between Comparative Example 4 and Example 4 is that no dandelion is added to the raw materials of the Chinese medicine composition.

[0173] Comparative Example 5

[0174] The only difference between Comparative Example 5 and Example 5 is that Zanthoxylum bungeanum, Stemona tuberosum, Taraxacum officinale and Celosia cristata are not added to the raw materials of the Chinese medicine composition.

[0175] Comparative Example 6

[0176] The only difference between Comparative Example 6 and Example 5 is that the decoctions in step (4) and step (6) are each performed only once.

[0177] Comparative Example 7

[0178] The only difference between Comparative Example 7 and Example 5 is that the amount of ethanol used in step (6) is 5 times the total amount of Stemona and Taraxacum.

[0179] Comparative Example 8

[0180] The only difference between Comparative Example 8 and Example 5 is that the amount of ethanol used in step (6) is 15 times the total amount of Stemona and Taraxacum.

[0181] Comparative Example 9

[0182] The difference between Comparative Example 9 and Example 5 is that step (6) is eliminated, and the Stemona and Taraxacum in the original step (6) are decocted together with the medicinal materials in step (4), and the decocted medicinal liquid is processed according to the original steps (7) and (8).

[0183] Comparative Example 10

[0184] The only difference between Comparative Example 10 and Example 5 is that no activated carbon adsorption is used in step (7).

[0185] Test Example 1

[0186] In vitro antibacterial experiment: The antibacterial effects of the traditional Chinese medicine compositions prepared in Examples 1-10 of the present invention and Comparative Examples 1-10 were evaluated according to WS / T650-2019 “Antibacterial and Antibacterial Effect Evaluation Method”.

[0187] The dosage form of the Chinese medicine composition provided by the present invention is a lotion or a spray, which belongs to a liquid preparation. The evaluation method of the antibacterial effect thereof is a suspension quantitative antibacterial experiment. The experimental strains are Staphylococcus aureus (ATCC6538), Escherichia coli (8099), Candida albicans (ATCC10231), Candida neoformans (ATCC32264), and Pseudomonas aeruginosa (ATCC27853). The antibacterial effect experimental results are shown in Table 1:

[0188] Table 1

[0189]

[0190]

[0191] It can be seen from Table 1 that the Chinese medicine composition obtained in Examples 1-10 of the present invention can achieve an inhibition rate of more than 99.85% against Staphylococcus aureus, Escherichia coli and Candida albicans under the synergistic effect of various raw materials, an inhibition rate of more than 99.50% against Candida novelta, and an inhibition rate of more than 99.3% against Pseudomonas aeruginosa. This indicates that the Chinese medicine composition provided by the present invention can inhibit and kill a variety of pathogens.

[0192] In Comparative Examples 1-10, due to the changes in raw material components and process, the antibacterial rate of the traditional Chinese medicine composition was reduced.

[0193] Test Example 2

[0194] Experiment on the effect of 5-hydroxytryptamine-induced pruritus in mice: The effect of the Chinese medicine compositions prepared in Examples 1-5 of the present invention and Comparative Examples 1-5 on 5-hydroxytryptamine-induced pruritus in mice was evaluated, and a blank group and a control group were set for comparison. The calculation method of the pruritus inhibition rate is as follows:

[0195] Itch inhibition rate = (number of scratching of mice in the blank group - number of scratching of mice in the experimental group) / number of scratching of mice in the blank group × 100%.

[0196] The specific experimental process is as follows:

[0197] 120 female mice from Kunming, weighing 25±2g, were selected and randomly divided into 13 experimental groups with 10 mice as a group. Experimental groups 1-5 used the Chinese medicine composition obtained in Example 1-5, experimental groups 6-10 used the Chinese medicine composition obtained in Comparative Examples 1-5, the control group (i.e., experimental group 11, hereinafter referred to as Control Group 1) selected Baicao Fuyan cleaning solution purchased on the market, and the blank group (i.e., experimental group 12, hereinafter referred to as blank group) selected physiological saline with the same volume as the above-mentioned experimental groups 1-11. It should be noted that the Chinese medicine compositions obtained in Examples 1-5 and Comparative Examples 1-5 are mixed evenly with clean water in a ratio of 1:10 when used and are set aside (when the Chinese medicine compositions provided by the embodiments and comparative examples of the present invention are used in subsequent test examples, they are mixed evenly with clean water in a ratio of 1:10 for standby use, which will not be repeated).

[0198] Before the experiment, the mice were adaptively raised for 3 days, and then the right ears of the mice were sprayed with drugs. The dosage was appropriate to coat the entire right ear of the mice, twice a day, and the drug was administered for 5 consecutive days. One hour after the last administration, 0.02 ml of 5 mol / L 5-hydroxytryptamine was subcutaneously injected into the right ears of the mice. After the injection, the number of times the mice scratched their right ears within 1 hour was counted, and the itching inhibition rate of the drugs in each experimental group was calculated.

[0199] The experimental results of each experimental group in Test Example 2 are shown in Table 2:

[0200] Table 2

[0201] Number of mice Number of scratches Itch suppression rate Experimental Group 1 10 13.9±1.2 76.91％ Experimental Group 2 10 13.5±1.0 77.57％ Experimental Group 3 10 13.4±1.2 77.74％ Experimental Group 4 10 14.1±1.9 76.58％ Experimental Group 5 10 14.3±2.7 76.24％ Experimental Group 6 10 19.2±2.1 68.11％ Experimental Group 7 10 25.4±2.5 57.81％ Experimental Group 8 10 18.2±1.9 69.76％ Experimental Group 9 10 30.1±2.6 50.00％ Experimental Group 10 10 17.4±1.7 71.10％ Control group 10 26.3±2.6 56.31％ Blank Group 10 60.2±3.2

[0202] It can be seen from Table 2 that the Chinese medicine composition provided by the present invention can effectively reduce the scratching frequency of mice after injection of 5-HT, and the itch inhibition rate of the Chinese medicine compositions in Examples 1-5 on mice is all above 75%.

[0203] Test Example 3

[0204] Experiment on the effect on cotton ball granuloma in rats: The effect of the Chinese medicine composition prepared in Examples 6-8 of the present invention and Comparative Examples 6-8 on cotton ball granuloma in rats was evaluated, and a blank group and control group 1 and control group 2 were set for comparison.

[0205] The specific experimental process is as follows:

[0206] 90 Wistar female rats weighing 200±20g were selected and randomly divided into 9 experimental groups with 10 rats as a group. Experimental groups 1-3 selected the Chinese medicine composition obtained in Examples 6-8, experimental groups 4-6 selected the Chinese medicine composition obtained in Comparative Examples 6-8, control group 1 and control group 2 (i.e., experimental group 7 and experimental group 8) each selected a purchased Baicao Fuyan cleaning liquid, and the blank group (experimental group 9) selected physiological saline with the same volume as the above experimental groups.

[0207] The rats were fed for 3 days before the experiment. Three days later, the rats were intraperitoneally injected with sodium pentobarbital at a dose of 30 mg / kg. After anesthesia, the rats were fixed, the skin of the lower abdomen was disinfected, a midline incision was made, a sterile cotton ball weighing 10 mg was placed under the skin of the groin on both sides, and then the incision was sutured.

[0208] For two consecutive days after surgery, penicillin potassium salt was injected into the rats once a day. Each experimental group applied the drug to the wound on the second day after surgery, once a day for 15 consecutive days, with a dosage of 1000 mg / kg. The blank group (experimental group 9) used the same volume of saline to apply to the wound. The animals were killed 1 hour after the last administration, and the cotton ball granuloma was taken out and dried in an oven and weighed. The weight calculation method of the granuloma is as follows:

[0209] Granuloma weight = granuloma weight of dried cotton ball - original cotton ball weight.

[0210] The experimental results of each experimental group in Test Example 3 are shown in Table 3:

[0211] Table 3

[0212] Number of mice Dosage Granuloma weight (mg) Experimental Group 1 10 1000mg / kg 29.95±2.73 Experimental Group 2 10 1000mg / kg 30.07±2.96 Experimental Group 3 10 1000mg / kg 30.58±3.11 Experimental Group 4 10 1000mg / kg 32.97±3.62 Experimental Group 5 10 1000mg / kg 33.15±3.47 Experimental Group 6 10 1000mg / kg 32.58±3.11 Control group 1 10 1000mg / kg 33.09±3.75 Control group 2 10 1000mg / kg 32.86±3.41 Blank Group 10 1000mg / kg 36.71±3.56

[0213] It can be seen from Table 3 that the Chinese medicine composition provided by the present invention can significantly inhibit the proliferation of cotton ball granuloma in rats.

[0214] Test Example 4

[0215] Experiment on the effect on pain caused by pressure on the base of the tail of mice: The effect of the Chinese medicine composition prepared in Examples 9 and 10 of the present invention and Comparative Examples 9 and 10 on the pain caused by pressure on the base of the tail of mice was evaluated, and a control group and a blank group were set for control experiments.

[0216] The specific experimental process is as follows:

[0217] 100 female mice from Kunming were selected, weighing 25 ± 2g. The mice were adaptively raised for 3 days before the experiment, and the mice were subjected to a tenderness test three days later. The mouse tail root tenderness method was adopted, and 1 cm from the mouse tail root was selected as the tenderness point. The pain threshold was determined with a tenderness meter, and 70 female mice with a pain threshold within 10-45 were screened based on the screaming of the mouse due to pain in the tail. The 70 selected mice were randomly divided into 7 experimental groups with 10 as a group. Experimental group 1 and experimental group 2 respectively adopted the Chinese medicine composition obtained in Examples 9 and 10, and experimental group 3 and experimental group 4 respectively adopted the Chinese medicine composition obtained in Comparative Examples 9 and 10, and control group 1 (i.e., experimental group 5) adopted the Baicao Fuyan cleaning solution purchased from outside, and control group 2 (i.e., experimental group 6) selected normal saline, and blank group (i.e., experimental group 7) was not subjected to any treatment.

[0218] The pain threshold of the selected mice was measured again, and the average of the measured pain thresholds was used as the pre-drug pain threshold.

[0219] The mice in each experimental group were given medication at the tender point, the medication method was smearing, and the dosage was 1500 mg / kg. The pain threshold of the mice in each experimental group was measured again 1 hour after the medication.

[0220] The experimental results of each experimental group in Test Example 4 are shown in Table 4:

[0221] Table 4

[0222] Number of mice Dosage Post-drug pain threshold (g) Experimental Group 1 10 1500mg / kg 42.57±4.35 Experimental Group 2 10 1500mg / kg 42.93±5.01 Experimental Group 3 10 1500mg / kg 39.85±4.73 Experimental Group 4 10 1500mg / kg 36.92±5.32 Control group 1 10 1500mg / kg 38.41±4.83 Control group 2 10 1500mg / kg 32.27±4.55 Blank Group 10 No medication 32.39±4.32

[0223] It can be seen from Table 4 that the Chinese medicine compositions obtained in Examples 9 and 10 of the present invention have a significant analgesic effect on the pain caused by pressure on the tail root of mice, and can increase the pressure of tenderness.

[0224] Test Example 5

[0225] Experiment on stimulation of rat vaginal mucosa: 30 SD female rats weighing 220±20g were selected. After adaptive feeding for 3 days before the experiment, the selected 30 rats were randomly divided into 3 experimental groups with 10 rats in a group. Among them, experimental group 1 used sterilized cotton wool of the same size as the rat vagina to soak the Chinese medicine composition obtained in Example 1. The soaking amount was preferably enough to wet the cotton wool completely. After soaking, the cotton wool was placed in the rat vagina. Experimental group 2 used sterilized cotton wool of the same size as the rat vagina to soak physiological saline. The soaking amount was preferably enough to wet the cotton wool completely. After soaking, the cotton wool was placed in the rat vagina. Experimental group 3 did not perform any treatment. After the cotton wool was placed for 24 hours, the rats were killed, the vagina was taken out, and the appearance of the rat vagina was observed. The results are shown in Table 5:

[0226] Table 5

[0227] Is there any swelling? Is there erosion? Is there congestion? Other phenomena Experimental Group 1 no no no no Experimental Group 2 no no no no Experimental Group 3 no no no no

[0228] It can be seen from Table 5 that the vaginas of the rats in the three experimental groups did not show congestion, swelling, erosion and the like, indicating that the Chinese medicine composition provided by the present invention has no irritation to SD female rats.

[0229] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some or all of the technical features therein may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for gynecological diseases, characterized in that: It is composed of the following raw materials in the following weight proportions: 50-150 parts of Fructus Cnidium, 20-50 parts of Herba Schizonepetae, 50-150 parts of Cortex Dictamni, 20-50 parts of Saposhnikoviae, 50-150 parts of Cortex Phellodendri, 40-80 parts of Sophora flavescens, 20-50 parts of Gentiana, 5-20 parts of Zanthoxylum bungeanum, 10-20 parts of Radix Stemonae, 10-30 parts of Flos Celosiae, and 20-50 parts of Herba Taraxaci.

2. The Chinese medicine composition for gynecological diseases according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in proportion by weight: 70-120 parts of Cnidium monnieri, 30-40 parts of Schizonepeta tenuifolia, 70-120 parts of Dictamni cortex, 30-40 parts of Saposhnikovia divaricata, 70-120 parts of Phellodendron chinense, 50-70 parts of Sophora flavescens, 30-40 parts of Gentiana scabra, 5-10 parts of Zanthoxylum bungeanum, 10-20 parts of Stemona tuberosum, 10-30 parts of Celosia cristata and 20-30 parts of Taraxacum officinale.

3. The Chinese medicine composition for gynecological diseases according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in proportion by weight: 100 parts of Cnidium monnieri, 30 parts of Schizonepeta tenuifolia, 100 parts of Dictamni cortex, 30 parts of Saposhnikovia divaricata, 100 parts of Phellodendron chinense, 60 parts of Sophora flavescens, 30 parts of Gentiana scabra, 5 parts of Zanthoxylum bungeanum, 10 parts of Stemona tuberosum, 10 parts of Celosia cristata and 20 parts of Taraxacum officinale.

4. The Chinese medicine composition for gynecological diseases according to claim 1, characterized in that: The dosage form of the Chinese medicine composition is one of lotion, liniment and spray.

5. A method for preparing a Chinese medicine composition for gynecological diseases according to any one of claims 1 to 4, characterized in that: The steps include: (1) Weigh the following raw materials in parts by weight: 50-150 parts of Cnidium monnieri, 20-50 parts of Schizonepeta tenuifolia, 50-150 parts of Dictamni cortex, 20-50 parts of Saposhnikovia divaricata, 50-150 parts of Phellodendron chinense, 40-80 parts of Sophora flavescens, 20-50 parts of Gentiana scabra, 5-20 parts of Zanthoxylum bungeanum, 10-20 parts of Stemona tuberosum, 10-30 parts of Celosia cristata, and 20-50 parts of Taraxacum officinale; (2) taking Nepeta tenuifolia, Saposhnikovia divaricata, and Cnidium monnieri, and processing them by steam distillation, collecting the distillate, and filtering the remaining material to obtain volatile oil A, aqueous solution B, and medicinal residue C, respectively; (3) mixing the volatile oil A and the aqueous solution B, adding polysorbate 80 thereto, and mixing well to obtain a clear drug solution D; (4) boiling the residue C with Dictamni cortex, Phellodendron amurense, Sophora flavescens, Gentiana scabra, Zanthoxylum bungeanum, and Celosia cristata with water, and filtering the mixture to obtain a concentrated solution E; (5) adding ethanol to the concentrated solution E and allowing the solution to stand, and then filtering the solution to obtain an alcohol precipitate F; (6) boiling the stemonae and dandelions in an ethanol solution and filtering the resulting mixture; boiling the residue in an ethanol solution again and filtering the resulting mixture; combining the two filtrates to obtain an ethanol extract G; (7) recovering ethanol from the alcohol precipitation solution F and the alcohol extract solution G, adding activated carbon to the solution after ethanol recovery for adsorption, and then filtering to obtain a concentrated solution H and a concentrated solution I, respectively; (8) The clarified medicinal solution D, the concentrated solution H and the concentrated solution I are mixed and a preservative is added to obtain the Chinese medicine composition.

6. The method for preparing the Chinese medicine composition for gynecological diseases according to claim 5, characterized in that: The amount of ethanol added in step (5) is based on the alcohol content in the concentrated solution E reaching 65%; The amount of ethanol added in step (6) is 9-13 times the weight of the medicinal material, and the concentration of ethanol is 75%.

7. The method for preparing the Chinese medicine composition for gynecological diseases according to claim 5, characterized in that: In the step (4), water is added and boiled for three times, the first time for two hours, the second time for 1.5 hours, and the third time for 1 hour. After boiling, the medicinal liquids are combined.

8. The method for preparing the Chinese medicine composition for gynecological diseases according to claim 5, characterized in that: The relative density of the concentrated solution E is 1.10-1.20 (70° C.).

9. The method for preparing the Chinese medicine composition for gynecological diseases according to claim 5, characterized in that: The Chinese medicine composition needs to be mixed with clean water in a ratio of 1:10 before use.

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