Cell incubator

Abstract

The invention discloses a cell culture box which comprises a box body, a box door rotationally arranged on the box body, a shading shell fixedly arranged in the box body, a mounting disc arranged in the shading shell, a static platform fixedly arranged on the mounting disc and a rotating platform rotating around the static platform. Culture dishes are arranged on the static platform and the rotating platform; the static platform is of a multi-layer structure, different light sources with adjustable brightness are arranged above each layer of the static platform, and the rotary platform realizes regulation and control of the light sources and dynamic cell culture. A culture dish dynamic moving structure is designed, part of culture dishes can be sequentially switched between an illumination area and a dark area, and simulation of a gradual change or periodic light environment is achieved. The incubator is divided into a plurality of layers, and each layer can be independently provided with an illumination condition, so that a multivariable contrast experiment is realized.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture box. Background Art

[0002] Cell culture incubators are essential equipment in life science research, used to simulate the environmental conditions required for cell growth in vitro. Currently, cell culture incubators on the market are mainly based on a constant environment, with stable control functions for light, temperature, humidity and carbon dioxide concentration.

[0003] In experiments involving illumination, traditional incubators have limitations. Traditional incubators are usually equipped with only fixed light sources, and the illumination intensity is fixed and non-adjustable, making it difficult to simulate the dynamic changes in light intensity in nature; it is impossible to study the light response mechanism of specific cells. In illumination experiments, many studies need to simulate the effects of dynamic day and night illumination changes, gradual light environments, or light cycles on cells. However, existing illumination culture equipment usually uses fixed culture racks and light sources, and experimental samples can only be exposed to a single illumination condition, and it is impossible to dynamically switch light zones or simulate light cycle changes. It is difficult to meet the needs of complex experiments, such as plant photoperiod regulation, animal biological clock research, etc. In traditional incubators, if multiple variable comparison experiments (such as different light intensities and photoperiod combinations) need to be carried out simultaneously, multiple incubators are usually required to set conditions separately. This method is not only costly and inefficient, but also different environments affect the reliability of experimental results. Therefore, the present invention proposes a cell culture incubator. Summary of the invention

[0004] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0005] In view of the inconvenience of light adjustment in the above-mentioned existing cell culture incubators, a new type of cell culture incubator is proposed.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a cell culture box, comprising a box body, a box door rotatably arranged on the box body, a light-shielding shell fixedly arranged in the box body, a mounting plate arranged in the light-shielding shell, a stationary platform fixedly arranged on the mounting plate, and a rotating platform rotating around the stationary platform; culture dishes are respectively arranged on the stationary platform and the rotating platform; the stationary platform is a multi-layer structure, and a light source with adjustable brightness is arranged above each stationary platform. The rotation of the rotating platform causes the culture dishes on it to be irradiated by different light sources, thereby realizing the regulation of the light source and dynamic cell culture.

[0007] As a preferred embodiment of the cell culture box of the present invention, a mounting part is arranged between the box body and the rotating platform; the mounting part includes a support shaft arranged in the box body and a mounting frame arranged at one end of the support shaft; a plurality of rotating platforms are rotatably arranged at the end of the mounting frame.

[0008] As a preferred solution of the cell culture box of the present invention, a support clamp is provided between the box door and the static platform.

[0009] As a preferred embodiment of the cell culture incubator described in the present invention, a sliding member is arranged between the rotating platform and the mounting frame, and the sliding member includes a sliding track connected to the box body, a rotating block slidably arranged in the sliding track, a connecting rod arranged between the rotating block and the rotating platform, and the rotating platforms are connected by a balance rod; the connecting rod passes through the end of the mounting frame and is rotatably arranged.

[0010] As a preferred embodiment of the cell culture box of the present invention, an intermittent component is arranged between the sliding member and the box body, and the intermittent component includes a trigger member arranged on the box body for rotation, a limit member connected to the box body, and a transmission member arranged between the trigger member and the limit member.

[0011] As a preferred embodiment of the cell culture box of the present invention, the trigger member includes a through hole opened on the box body, a rotating shaft rotatably arranged in the through hole, a trigger block sleeved on one end of the rotating shaft, and a trigger slope arranged at the end of the trigger block.

[0012] As a preferred embodiment of the cell culture box described in the present invention, the limiting member includes a mounting wheel connected to the mounting frame, a limiting groove opened on the mounting wheel, a sliding plate arranged on the inner side of the box body, a sliding groove opened on the sliding plate, a sliding block slidably arranged in the sliding groove, and a compression spring arranged between the sliding block and the sliding groove.

[0013] As a preferred solution of the cell culture box of the present invention, the end of the sliding block is an inclined surface, and the inclined surface of the sliding block can be engaged with the limiting groove.

[0014] As a preferred embodiment of the cell culture incubator of the present invention, the transmission member includes a mounting protrusion arranged on the trigger block, a spring block arranged on the mounting wheel, a tension spring arranged between the mounting protrusion and the spring block, and a limiting protrusion arranged on the mounting wheel.

[0015] As a preferred solution of the cell culture incubator of the present invention, one end of the rotating shaft is connected to a power source.

[0016] Beneficial effects of the cell culture box of the present invention: The present invention designs a dynamic moving structure of the culture dish, and some culture dishes can switch between the light area and the dark area in sequence to achieve the simulation of a gradual or periodic light environment. The culture box is divided into multiple layers, and each layer can independently set the light conditions to achieve a multivariate comparison experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0018] Figure 1 It is a schematic diagram of the overall structure of the cell culture box of the present invention.

[0019] Figure 2 This is an exploded view of the overall structure of the cell culture box of the present invention.

[0020] Figure 3 This is an enlarged view of the sliding structure of the cell culture box of the present invention.

[0021] Figure 4 This is a schematic diagram of the mounting structure of the cell culture box of the present invention.

[0022] Figure 5 This is an enlarged view of the limiting structure of the cell culture box of the present invention.

[0023] Figure 6 This is an enlarged view of the transmission structure of the cell culture box of the present invention. DETAILED DESCRIPTION

[0024] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0025] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0026] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0027] Secondly, the present invention is described in detail with reference to the schematic diagram. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0028] Example 1

[0029] Reference Figures 1 to 4 Provided is a schematic diagram of the overall structure of a cell culture box, a cell culture box comprising a box body 100, a box door 101 rotatably arranged on the box body 100, a light-shielding shell 102 fixedly arranged in the box body 100, a mounting plate 103 arranged in the light-shielding shell 102, a stationary platform 104 fixedly arranged on the mounting plate 103, and a rotating platform 105 rotating around the stationary platform 104; culture dishes 106 are respectively arranged on the stationary platform 104 and the rotating platform 105; the stationary platform 104 is a multi-layer structure, and a light source with adjustable brightness is arranged above each layer of the stationary platform 104, and the rotating platform 105 rotates so that the culture dishes 106 thereon are irradiated by different light sources, thereby realizing the regulation of the light source and dynamic cell culture. In this embodiment, the stationary platform 104 fixedly arranged on one side of the mounting plate 103 is divided into three layers, with a large middle layer and small upper and lower layers, so that the six rotating platforms 105 can rotate around the edges of the three stationary platforms 104, wherein the light source of the upper layer of the stationary platform 104 is arranged on the top inner side of the box body 100, the light source of the middle layer of the stationary platform 104 is arranged below the upper layer of the stationary platform 104, and the light source of the lower layer of the stationary platform 104 is arranged below the middle layer of the stationary platform 104.

[0030] Furthermore, a mounting member 107 is provided between the box 100 and the rotating platform 105; the mounting member 107 comprises a support shaft 107a provided in the box 100, and a mounting frame 107b provided at one end of the support shaft 107a; and a plurality of rotating platforms 105 are rotatably provided at the end of the mounting frame 107b. In this embodiment, the support shaft 107a is rotatably connected to the inner side of the box 100, and the mounting frame 107b is in the shape of a hexagonal star, and a rotating platform 105 is provided at the end of each corner.

[0031] A support clamp 110 is provided between the box door 101 and the stationary platform 104. In this embodiment, a support clamp 110 is fixedly provided on the inner side of the box door 101, and the support clamp 110 is divided into an upper support clamp and a lower support clamp. When the box door 101 is closed, the upper support clamp and the lower support clamp clamp the upper and lower surfaces of the middle layer of the stationary platform 104 respectively, so that the mounting plate 103 is fixed in the light shielding shell 102 and does not rotate.

[0032] Furthermore, a sliding member 108 is provided between the rotating platform 105 and the mounting frame 107b, and the sliding member 108 includes a sliding track 108a connected to the box 100, a rotating block 108b slidably disposed in the sliding track 108a, a connecting rod 108c disposed between the rotating block 108b and the rotating platform 105, and the rotating platforms 105 are connected by a balancing rod 108d; the connecting rod 108c penetrates and is rotatably disposed at the end of the mounting frame 107b. In this embodiment, one side of each two rotating platforms 105 is rotatably connected by a connecting rod 108c, and the other side of the rotating platform 105 is rotatably connected to the connecting rod 108c. Through the mutual cooperation of the connecting rod 108c, the balancing rod 108d, the sliding block 109b-5 and the sliding track 108a, when the rotating platform 105 rotates, the upper surface of the rotating platform 105 is always facing upward.

[0033] Operation process: When the support shaft 107a rotates, it drives the mounting plate 103 to rotate, and the mounting plate 103 drives the rotating platform 105 to rotate around the stationary platform 104. Through the cooperation of the connecting rod 108c, the balance rod 108d, the sliding block 109b-5 and the sliding track 108a, the upper surface of the rotating platform 105 always faces upward. Even when the rotating platform 105 rotates, the cell culture dish 106 can be stably placed on the rotating platform 105.

[0034] Beneficial effects: The cell culture incubator is divided into three layers, each layer can be set to a different light zone. Cells can be cultured in the same light zone on the stationary platform 104, and cells can be dynamically cultured in different light zones on the rotating platform 105. The illumination intensity of the three layers of the cell culture incubator is changed to strong, weak, and dark to simulate the dynamic changes of light intensity in nature, so as to realize the research on plant photoperiod regulation and animal biological clock. After the experiment, the cells in the stationary platform 104 and the rotating platform 105 can be compared to verify the effects of different light source environments on cell growth. Since comparative experiments usually require the use of multiple cell culture incubators, the use of cell culture incubators is reduced. Since the cells grow in the same cell culture incubator, the temperature and humidity are consistent, which also avoids the use of multiple different cell culture incubators. The different environments may lead to inconsistent experimental results.

[0035] Example 2

[0036] Reference Figures 4 to 6This embodiment is different from the first embodiment in that an intermittent component 109 is provided between the sliding member 108 and the box body 100, and the intermittent component 109 includes a trigger member 109a which is provided on the box body 100 for rotation, a limit member 109b which is connected to the box body 100, and a transmission member 109c which is provided between the trigger member 109a and the limit member 109b.

[0037] Specifically, the trigger member 109a includes a through hole 109a-1 opened on the box body 100, a rotating shaft 109a-2 rotatably disposed in the through hole 109a-1, a trigger block 109a-3 sleeved on one end of the rotating shaft 109a-2, and a trigger inclined surface 109a-4 disposed at the end of the trigger block 109a-3. In this embodiment, the trigger block 109a-3 and the end inclined surface of the trigger block 109a-3 form a trapezoidal block, which is fixedly disposed at one end of the rotating shaft 109a-2 and rotates with the rotating shaft 109a-2.

[0038] Furthermore, the limiting member 109b includes a mounting wheel 109b-1 connected to the mounting frame 107b, a limiting groove 109b-2 opened on the mounting wheel 109b-1, a sliding plate 109b-3 arranged on the inner side of the box body 100, a sliding groove 109b-4 opened on the sliding plate 109b-3, a sliding block 109b-5 slidably arranged in the sliding groove 109b-4, and a compression spring 109b-6 arranged between the sliding block 109b-5 and the sliding groove 109b-4. In this embodiment, the mounting frame 107b is fixedly connected to the mounting wheel 109b-1 through the support shaft 107a, and there are six limit grooves 109b-2, sliding blocks 109b-5 and compression springs 109b-6, which are equidistantly distributed on the inner side of the sliding disk 109b-3. The compression spring 109b-6 is in a compressed state, so that the end of the sliding block 109b-5 is in conflict with the surface of the mounting wheel 109b-1.

[0039] Furthermore, the end of the sliding block 109b-5 is an inclined surface, and the inclined surface of the sliding block 109b-5 can be engaged with the limiting groove 109b-2.

[0040] The transmission member 109c includes a mounting protrusion 109c-1 disposed on the trigger block 109a-3, a spring block 109c-2 disposed on the mounting wheel 109b-1, a tension spring 109c-3 disposed between the mounting protrusion 109c-1 and the spring block 109c-2, and a limiting protrusion 109c-4 disposed on the mounting wheel 109b-1. The trigger block 109a-3 and the limiting protrusion 109c-4 are 60 degrees apart.

[0041] Furthermore, one end of the rotating shaft 109a-2 is connected to a power source.

[0042] The rest of the structure is the same as that of Example 1.

[0043] Operation process: rotate the rotating shaft 109a-2, the rotating shaft 109a-2 drives the trigger block 109a-3 to rotate, and the mounting protrusion 109c-1 stretches the tension spring 109c-3 between the mounting protrusion 109c-1 and the spring block 109c-2. Since the inclined surface of the sliding block 109b-5 is engaged with the limiting groove 109b-2, the mounting wheel 109b-1 does not move. When the end of the trigger block 109a-3 hits the end of the sliding block 109b-5, the sliding block 109b-5 is pushed out of the limiting groove 109b-2, and the tension spring 109c-3 contracts, pulling the mounting wheel 109b-1 to rotate. Then the limiting block hits the trigger block 109a-3, and the mounting wheel 109b-1 stops rotating. Since the trigger block 109a-3 and the limiting protrusion 109c-4 are 60 degrees, the mounting wheel 109b-1 rotates 60 degrees.

[0044] Beneficial effects: An intermittent component 109 is provided between the sliding member 108 and the box body 100, which enables the rotating platform 105 to rotate to the edge of the stationary platform 104 more quickly. When the rotating platform 105 moves, the influence of different light intensities of the stationary platform 104 on the cell culture through the gap during rotation is reduced. The rotation angle of the rotating platform 105 can also be controlled so that the rotating platform 105 can stop at the edge of the stationary platform 104 each time it rotates, thereby reducing the gap between the rotating platform 105 and the stationary platform 104, and making the light of each layer in the box body 100 less easily affected.

[0045] Importantly, it should be noted that the construction and arrangement of the present application shown in a plurality of different exemplary embodiments are only exemplary. Although only a few embodiments are described in detail in this disclosure, it should be readily understood by those who refer to this disclosure that many modifications are possible (e.g., the size, scale, structure, shape and proportion of various elements, and parameter values ​​(e.g., temperature, pressure, etc.), installation arrangement, use of materials, color, directional changes, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in the application. For example, the element shown as integrally formed can be composed of multiple parts or elements, the position of the element can be inverted or otherwise changed, and the nature or number or position of the discrete element can be changed or changed. Therefore, all such modifications are intended to be included in the scope of the present invention. The order or sequence of any process or method steps can be changed or reordered according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure of performing the function described herein, and is not only structurally equivalent but also equivalent structure. Without departing from the scope of the present invention, other replacements, modifications, changes and omissions can be made in the design, operating conditions and arrangement of the exemplary embodiments. Therefore, the invention is not limited to a specific embodiment, but extends to numerous modifications still falling within the scope of the appended claims.

[0046] Additionally, in order to provide a concise description of exemplary embodiments, all features of an actual embodiment (ie, those features that are not relevant to the best mode presently contemplated for carrying out the invention or those that are not relevant to implementing the invention) may not be described.

[0047] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

[0048] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A cell culture incubator, characterized in that: The invention comprises a box body (100), a box door (101) rotatably arranged on the box body (100), a light shielding shell (102) fixedly arranged in the box body (100), a mounting plate (103) arranged in the light shielding shell (102), a stationary platform (104) fixedly arranged on the mounting plate (103), and a rotating platform (105) rotating around the stationary platform (104); A culture dish (106) is respectively arranged on the stationary platform (104) and the rotating platform (105); The stationary platform (104) is a multi-layer structure, and a light source with adjustable brightness is provided above each layer of the stationary platform (104). By rotating the rotating platform (105), the culture dish (106) on it is illuminated by different light sources, thereby realizing the regulation of the light source and dynamic cell culture.

2. The cell culture incubator according to claim 1, characterized in that: A mounting member (107) is provided between the box (100) and the rotating platform (105); the mounting member (107) comprises a support shaft (107a) provided in the box (100) and a mounting frame (107b) provided at one end of the support shaft (107a); A plurality of rotating platforms (105) are rotatably arranged at the end of the mounting frame (107b).

3. The cell culture incubator according to claim 1, characterized in that: A supporting clamp (110) is provided between the box door (101) and the stationary platform (104).

4. The cell culture incubator according to claim 2, characterized in that: A sliding member (108) is provided between the rotating platform (105) and the mounting frame (107b), and the sliding member (108) comprises a sliding track (108a) connected to the box (100), a rotating block (108b) slidably arranged in the sliding track (108a), and a connecting rod (108c) arranged between the rotating block (108b) and the rotating platform (105). The rotating platforms (105) are connected via a balancing rod (108d); The connecting rod (108c) is rotatably disposed through the end of the mounting frame (107b).

5. The cell culture incubator according to claim 4, characterized in that: An intermittent component (109) is arranged between the sliding member (108) and the box body (100), and the intermittent component (109) comprises a trigger member (109a) arranged on the box body (100) for rotation, a limit member (109b) connected to the box body (100), and a transmission member (109c) arranged between the trigger member (109a) and the limit member (109b).

6. The cell culture incubator according to claim 5, characterized in that: The trigger member (109a) comprises a through hole (109a-1) opened on the box body (100), a rotating shaft (109a-2) rotatably arranged in the through hole (109a-1), a trigger block (109a-3) sleeved on one end of the rotating shaft (109a-2), and a trigger inclined surface (109a-4) arranged at the end of the trigger block (109a-3).

7. The cell culture incubator according to claim 6, characterized in that: The limiting member (109b) comprises a mounting wheel (109b-1) connected to the mounting frame (107b), a limiting groove (109b-2) provided on the mounting wheel (109b-1), a sliding plate (109b-3) arranged on the inner side of the box body (100), a sliding groove (109b-4) provided on the sliding plate (109b-3), a sliding block (109b-5) slidably arranged in the sliding groove (109b-4), and a compression spring (109b-6) arranged between the sliding block (109b-5) and the sliding groove (109b-4).

8. The cell culture incubator according to claim 7, characterized in that: The end of the sliding block (109b-5) is an inclined surface, and the inclined surface of the sliding block (109b-5) can be engaged with the limiting groove (109b-2).

9. The cell culture incubator according to claim 8, characterized in that: The transmission member (109c) comprises a mounting protrusion (109c-1) arranged on the trigger block (109a-3), a spring block (109c-2) arranged on the mounting wheel (109b-1), a tension spring (109c-3) arranged between the mounting protrusion (109c-1) and the spring block (109c-2), and a limiting protrusion (109c-4) arranged on the mounting wheel (109b-1).

10. The cell culture incubator according to claim 6, wherein: One end of the rotating shaft (109a-2) is connected to a power source.

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