Liver organ chip-based anti-fibrosis active component high-throughput screening method and application thereof

Abstract

The invention discloses an anti-fibrosis active ingredient high-throughput screening method based on a liver organ chip and application of the anti-fibrosis active ingredient high-throughput screening method. The invention provides a liver organ chip combined with a pore plate. The liver organ chip is used for high-throughput screening of anti-fibrosis active ingredients. The method specifically comprises the steps of building a high-throughput organ-like chip, forming a 3D fibrosis liver organ and screening the biological activity of an anti-fibrosis drug. According to the invention, the physiological state of the human liver is reproduced in an in-vitro culture environment by utilizing the principles of stem cell development and engineering, and the pharmacological action of the liver tissue on the medicines obeticholic acid and semeglutide under the pathological state of the human liver is remodeled; the method has great application value in the aspects of in-vitro organ simulation, high-throughput screening of drug efficacy and exploration of a response mechanism of an organism to a drug, and the construction mode of combining with a commercial pore plate is extremely simple, convenient, expansible and practical.

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