Probe preparation method, probe and optical sensing system

Abstract

The embodiment of the invention discloses a preparation method of a probe, the probe and an optical sensing system.The preparation method comprises the steps that firstly, a sol-gel solution is prepared, and an indicator is doped in the sol-gel solution; then coating the sol-gel liquid on a preset area of a probe to form a sensing film; the sensing film is used for monitoring physiological index parameters. By means of the method, the prepared sol-gel liquid doped with the indicator is coated on the preset area of the probe, the sensing film is formed in the preset area, different types of indicators correspond to different types of sensing films, monitoring of physiological index parameters can be achieved through the probe and the sensing film on the probe, and the accuracy of physiological index monitoring is improved. According to the present invention, the rapid change of the biological tissue fluid dissolved oxygen partial pressure, the pH value and the like can be accurately determined, and the probe has advantages of small size, simple detection process, easy operation, sensitive and rapid reaction, good linear relationship, good repeatability, good reversibility and the like.

Description

Technical Field

[0001] The embodiments of the present invention relate to the technical field of optical devices, and in particular to a method for preparing a probe, a probe, and an optical sensing system. Background Art

[0002] pH detection and dissolved oxygen DO detection are the two most common detection methods at present, and are widely used in many fields, such as petroleum, chemical industry, clinical testing, engineering process monitoring, etc. Compared with electrochemical sensors, optical fiber sensors are used to detect pH and dissolved oxygen DO. Optical fiber sensors have the advantages of safety and explosion-proof, high precision, long-distance transmission, no fear of corrosion, and anti-electromagnetic interference.

[0003] However, the detection equipment using optical fiber sensors takes up a large space, is complicated to operate, and the detection process is time-consuming and labor-intensive. Moreover, often a detection device can only detect the pH value, or can only detect the dissolved oxygen DO, and cannot detect the pH value and the dissolved oxygen DO at the same time. Summary of the invention

[0004] The embodiment of the present invention provides a method for preparing a probe, a probe and an optical sensing system, so as to monitor physiological index parameters through the probe and a sensing film on the probe, and the probe is small in size, and the detection process is simple and easy to operate.

[0005] In a first aspect, an embodiment of the present invention provides a method for preparing a probe, comprising:

[0006] preparing a sol-gel solution, wherein the sol-gel solution is doped with an indicator;

[0007] The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film; the sensing film is used to monitor physiological index parameters.

[0008] Optionally, the probe comprises a multimode optical fiber, the multimode optical fiber comprises a core, a cladding and a coating layer, the cladding surrounds the core, and the coating layer surrounds the cladding;

[0009] Before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes:

[0010] At the preset area, removing the coating layer and the cladding layer of the multimode optical fiber until the fiber core is exposed;

[0011] The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising:

[0012] The sol-gel solution is applied on the exposed core to form the sensing film.

[0013] Optionally, the probe includes a plurality of the preset areas;

[0014] At the preset area, removing the coating layer and the cladding of the multimode optical fiber until the core is exposed, comprising:

[0015] At each of the preset areas, the coating layer and the cladding layer of the multimode optical fiber are removed respectively until the fiber core is exposed in each of the preset areas;

[0016] Applying the sol-gel solution on the exposed fiber core to form the sensing film comprises:

[0017] The sol-gel solution doped with different types of the indicator is respectively coated on the exposed fiber cores in each of the preset areas to form a plurality of different types of the sensing films.

[0018] Optionally, the probe comprises an optical fiber bundle and a capillary end, the capillary end comprises a first end and a second end opposite to each other, the first end is an open end, and the first end is used to insert the optical fiber bundle and be fixedly connected to the optical fiber bundle, and the second end is a closed spherical convex bottom structure;

[0019] Before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes:

[0020] Inserting one end of the optical fiber bundle into the first end, and gluing and fixing the first end to one end of the optical fiber bundle by using optical fiber matching liquid or UV acrylic glue;

[0021] The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising:

[0022] The sol-gel solution is coated on the surface of the capillary end to form the sensing film.

[0023] Optionally, the indicator doped in the sol-gel solution includes Ru(dpp)3Cl2 (4,7-diphenyl-1,10-phenanthroline ruthenium dichloride), and the sensing membrane includes a dissolved oxygen sensing membrane, and the dissolved oxygen sensing membrane is used to monitor the dissolved oxygen content;

[0024] The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising:

[0025] The sol-gel solution doped with the Ru(dpp)3Cl2 indicator is applied to and covers the preset area by an immersion-pulling method to form the dissolved oxygen sensing membrane for monitoring the dissolved oxygen content.

[0026] Optionally, the indicator doped in the sol-gel solution includes BCG (bromocresol green) or CNF (5(6)-carboxyfluorescein), and the sensing membrane includes a first pH value sensing membrane or a second pH value sensing membrane, and the first pH value sensing membrane and the second pH value sensing membrane are used to monitor pH value;

[0027] The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising:

[0028] By the immersion pulling method, the sol-gel solution doped with the BCG indicator or the CNF indicator is applied to and covers the preset area to form the first pH value sensing membrane or the second pH value sensing membrane for monitoring the pH value. In a second aspect, an embodiment of the present invention further provides a probe prepared by the preparation method of the probe as described in any one of the first aspects.

[0029] In a third aspect, an embodiment of the present invention further provides an optical sensing system, comprising a light source module, a detection module, and the probe as described in the second aspect, wherein the probe comprises a sensing film;

[0030] The light source module is connected to an end of the probe away from the sensing film, and the light source module is used to emit a light beam to the probe;

[0031] The detection module is connected to an end of the probe away from the sensing film, and the detection module is used to receive the light beam reflected by the probe.

[0032] Optionally, the light source module includes a plurality of light sources and a coupling lens group; each of the light sources is lit at the same time;

[0033] The light source is used to emit an LED light beam;

[0034] The coupling lens group is located on the optical path of the emitted light from the light source, and is used to couple the LED light beams emitted by each of the light sources into a composite light beam.

[0035] Optionally, the light source module includes a plurality of light sources, a plurality of plano-convex lenses, a dichroic mirror and an aspheric lens, the number of the light sources and the plano-convex lenses are the same and correspond one to one; each of the light sources is lit in time-sharing or simultaneously;

[0036] The light source is used to emit an LED light beam;

[0037] The plano-convex lens is located on the optical path of the outgoing light from the light source, and the plano-convex lens is used to collimate the LED light beam emitted by the light source into a beam of parallel light;

[0038] The dichroic mirror is located on the outgoing light path of the plano-convex lens, and the dichroic mirror is used to couple the parallel lights into a composite light.

[0039] The aspherical lens is located on the optical path of the outgoing light of the dichroic mirror, and the aspherical lens is used to collimate the composite light into a beam of parallel composite light.

[0040] The embodiment of the present invention provides a method for preparing a probe, a probe and an optical sensing system. The preparation method first prepares a sol-gel liquid, in which an indicator is doped; then the sol-gel liquid is applied to a preset area of ​​the probe to form a sensing film; the sensing film is used to monitor physiological index parameters. Using the above method, the prepared sol-gel liquid doped with an indicator is applied to a preset area of ​​the probe to form a sensing film at the preset area. Different types of indicators form different types of sensing films. The probe and the sensing film on the probe can be used to monitor physiological index parameters, and rapid changes in dissolved oxygen partial pressure and pH value of biological tissue fluid can be accurately measured. The probe has the advantages of small size, simple and easy detection process, sensitive and fast response, good linear relationship, good repeatability and reversibility, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 It is a schematic diagram of a process for preparing a probe provided by an embodiment of the present invention;

[0042] Figure 2 It is a schematic diagram of a process for preparing another probe provided by an embodiment of the present invention;

[0043] Figure 3 is a schematic diagram of the structure of a probe provided by an embodiment of the present invention;

[0044] Figure 4 is a schematic cross-sectional view of a multimode optical fiber provided by an embodiment of the present invention;

[0045] Figure 5 It is a schematic flow chart of another method for preparing a probe provided by an embodiment of the present invention;

[0046] Figure 6 is a schematic diagram of the structure of another probe provided by an embodiment of the present invention;

[0047] Figure 7 It is a schematic flow chart of another method for preparing a probe provided by an embodiment of the present invention;

[0048] Figure 8 is a schematic diagram of the structure of another probe provided by an embodiment of the present invention;

[0049] Fig. 9 is a schematic cross-sectional view of an optical fiber bundle provided by an embodiment of the present invention;

[0050] Fig.10 , Fig.11 and Fig.12 Schematic diagrams of the structures of three optical sensing systems provided by embodiments of the present invention;

[0051] Fig.13 is a structural schematic diagram of a light source module provided by an embodiment of the present invention;

[0052] Fig.14 is a structural schematic diagram of another light source module provided by an embodiment of the present invention;

[0053] Fig.15 It is a structural schematic diagram of another light source module provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0054] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only parts related to the present invention, rather than all structures, are shown in the accompanying drawings.

[0055] The terms used in the embodiments of the present invention are only for the purpose of describing specific embodiments, and are not intended to limit the present invention. It should be noted that the directional words such as "upper", "lower", "left", "right" and the like described in the embodiments of the present invention are described at the angles shown in the accompanying drawings and should not be understood as limitations on the embodiments of the present invention. In addition, in the context, it is also necessary to understand that when it is mentioned that an element is formed "on" or "under" another element, it can not only be directly formed "on" or "under" another element, but also indirectly formed "on" or "under" another element through an intermediate element. The terms "first", "second", etc. are only used for descriptive purposes and do not represent any order, quantity or importance, but are only used to distinguish different components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific circumstances.

[0056] The term “including” and its variations used in the present invention are open inclusions, that is, “including but not limited to.” The term “based on” means “based at least in part on.” The term “one embodiment” means “at least one embodiment.”

[0057] It should be noted that the concepts such as "first" and "second" mentioned in the present invention are only used to distinguish the corresponding contents, and are not used to limit the order or interdependence.

[0058] It should be noted that the modifications of "one" and "plurality" mentioned in the present invention are illustrative rather than restrictive, and those skilled in the art should understand that, unless otherwise clearly indicated in the context, it should be understood as "one or more".

[0059] Figure 1 is a schematic diagram of a method for preparing a probe provided by an embodiment of the present invention, such as Figure 1 As shown, the preparation method comprises:

[0060] S110, preparing a sol-gel solution, wherein the sol-gel solution is doped with an indicator.

[0061] Specifically, the preparation of the sol-gel solution can be understood as a process of uniformly mixing the indicator with the initial sol-gel mixture. For example, the mixing ratio of the indicator to the initial sol-gel mixture can be 0.5-10 mg / g, that is, 0.5-10 mg of the indicator can be mixed in 1 g of the initial sol-gel mixture. Among them, the indicator is mostly in powder or liquid form, has no adhesion, and the indicator cannot be directly applied to the preset area of ​​the probe. The initial sol-gel mixture can use a silane oxide compound (exemplarily, the silane oxide compound can be tetramethylsiloxane (TMOS), tetraethylsiloxane (TEOS), etc.) as a matrix, and can be modified with an organic alkoxide compound (exemplarily, the alkoxide compound can be dimethyldiethoxysilane (DMDES), phenyltriethylsiloxane (Ph-TriEOS), methyltrimethoxysilane (MTMOS), phenyltrimethoxysilane (PTMOS), isobutyltrimethoxysilane (iBu-TMOS), epoxytrimethoxysilane (GLYMO), etc.) to adjust the hydrophobicity of the base film and the gel network structure. Furthermore, the molar ratio of the organic alkoxy compound to the silaneoxy compound can be selected to be in the range of 0.1-1 for mixed modification, and a certain amount of ethanol and hydrochloric acid aqueous solution can be added, and the pH value of the initial sol-gel mixture can be adjusted to about 1-2 by the hydrochloric acid aqueous solution, and then an acid hydrolysis condensation reaction is carried out, wherein the reaction temperature range of the acid hydrolysis condensation reaction is 30-70°C, the reaction time range is 2-5h, and the room temperature aging time range is 1-10d.

[0062] S120, applying the sol-gel liquid to a preset area of ​​the probe to form a sensing film; the sensing film is used to monitor physiological index parameters.

[0063] It should be noted that the probe mentioned here can be understood as an initial probe, that is, an initial probe that has not undergone any processing and does not have a detection function. After the corresponding sensing film is formed on the preset area of ​​the probe, the probe and the sensing film thereon can be used together in the detection process to play the role of data detection. Specifically, the sol-gel liquid is applied to the preset area of ​​the probe. Since the sol-gel liquid is in a wet film state, it is also necessary to undergo room temperature drying and mechanical drying processes to remove the solvent and moisture in the sol-gel liquid, and further condense to obtain a porous dry film, which is the sensing film. Different types of indicators correspond to different types of sensing films. Different types of sensing films can realize the monitoring of physiological index parameters, wherein the physiological index parameters can be dissolved oxygen content and pH value, or blood sugar / glucose content, etc. For example, the probe in the embodiment of the present invention can be used to monitor the physiological index parameters in biological tissue fluid (specifically cerebrospinal fluid). For example, the flash drying time of the room temperature flash drying process is 5-20min, the drying time of the mechanical drying process is 1-5h, and the drying temperature is 60-100℃.

[0064] The technical solution in the embodiment of the present invention is that the preparation method first prepares a sol-gel liquid, in which an indicator is doped; then the sol-gel liquid is applied to a preset area of ​​the probe to form a sensing film; the sensing film is used to monitor physiological index parameters. Using the above method, the prepared sol-gel liquid doped with an indicator is applied to a preset area of ​​the probe to form a sensing film at the preset area. Different types of indicators correspond to different types of sensing films. The monitoring of physiological index parameters can be achieved through the probe and the sensing film on the probe. The rapid changes of dissolved oxygen partial pressure and pH value of biological tissue fluid can be accurately measured, and the probe has the advantages of small size, simple and easy to operate detection process, sensitive and fast response, good linear relationship, good repeatability and reversibility, etc.

[0065] In one embodiment, optionally, the indicator doped in the sol-gel liquid includes Ru(dpp)3Cl2 (4,7-diphenyl-1,10-phenanthroline ruthenium dichloride), and the sensing membrane includes a dissolved oxygen sensing membrane, which is used to monitor the dissolved oxygen content; the sol-gel liquid is applied to a preset area of ​​the probe to form a sensing membrane, including: applying and covering the preset area with the sol-gel liquid doped with Ru(dpp)3Cl2 indicator by an immersion pulling method to form a dissolved oxygen sensing membrane for monitoring the dissolved oxygen content.

[0066] Specifically, by the immersion and pulling method, the preset area of ​​the probe is vertically immersed in a sol-gel liquid, in which the Ru(dpp)3Cl2 indicator is doped. After a period of immersion, the preset area of ​​the probe is vertically pulled up from the sol-gel liquid at a low speed, and the sol-gel liquid attached to the preset area of ​​the probe covers the preset area. The formed sensing film is a dissolved oxygen sensing film, which can be used to monitor the dissolved oxygen content. In the detection process of the probe formed with the dissolved oxygen sensing film, the dissolved oxygen sensing film is immersed in the solution to be tested. Due to the quenching effect of oxygen, the fluorescence effect of the Ru(dpp)3Cl2 indicator at different wavelengths is related to the oxygen concentration. For example, an LED light source with a wavelength of 450nm is used to emit a light beam. The LED light source is incident on the dissolved oxygen sensing film and reflected by the dissolved oxygen sensing film. The fluorescence intensity of the Ru(dpp)3Cl2 indicator in the wavelength range of 600-640nm decreases with the increase of oxygen concentration. A calibration curve of the relationship between fluorescence intensity and oxygen concentration can be obtained according to the reflected light beam. Where I0 represents the fluorescence intensity of the system when oxygen is absent, I represents the fluorescence intensity of the system when oxygen is present, and K SV is the fluorescence enhancement constant, Indicates the dissolved oxygen concentration in the test solution, thereby determining the dissolved oxygen content / dissolved oxygen concentration in the test solution.

[0067] In another embodiment, optionally, the indicator doped in the sol-gel liquid includes BCG (bromocresol green) or CNF (5(6)-carboxyfluorescein), and the sensing membrane includes a first pH sensing membrane or a second pH sensing membrane, and the first pH sensing membrane and the second pH sensing membrane are used to monitor the pH value; the sol-gel liquid is applied to a preset area of ​​the probe to form a sensing membrane, including: by an immersion pulling method, the sol-gel liquid doped with BCG indicator or CNF indicator is applied and coated on the preset area to form a first pH sensing membrane or a second pH sensing membrane for monitoring the pH value.

[0068] Specifically, the indicator doped in the sol-gel liquid includes BCG (bromocresol green), and the sensing film includes a first pH sensing film, which is used to monitor the pH value; the sol-gel liquid is applied to the preset area of ​​the probe to form a sensing film, including: applying and covering the preset area with the sol-gel liquid doped with the BCG indicator by the immersion-pulling method to form a first pH sensing film for monitoring the pH value. By the immersion-pulling method, the preset area of ​​the probe is vertically immersed in the sol-gel liquid, which is doped with the BCG indicator. After immersion for a period of time, the preset area of ​​the probe is vertically pulled up from the sol-gel liquid at a low speed, and the sol-gel liquid attached to the preset area of ​​the probe covers the preset area. The sensing film formed is the first pH sensing film, which can be used to monitor the pH value. The first pH sensing film can also be called a white light sensing film. In the detection process of the probe formed with the first pH value sensing film, the first pH value sensing film is immersed in the solution to be tested. Since the BCG indicator absorbs white light in the wavelength range of 420-720nm, wherein the acid isomer has an absorption peak at 440-445nm, and the base isomer has an absorption peak at 615-625nm, and both have isosbestic points at 480-515nm, illustratively, an LED light source with an emission light beam wavelength range of 400-700nm is used, and the LED light source is incident on the first pH value sensing film and reflected by the first pH value sensing film. The intensity of the BCG indicator in the wavelength range of 615-625nm decreases with the increase of the pH value, and the calibration curve of the fluorescence intensity normalized by the isosbestic point and the pH value can be obtained. Wherein, I represents the normalized system strength of the isosbestic point when the BCG indicator is present, I0 represents the normalized system strength of the isosbestic point when the BCG indicator is not present, a and b are the calibration curve fitting coefficients of the intensity and pH value, a and b are constants, thereby determining the pH value in the test solution.

[0069] Alternatively, the indicator doped in the sol-gel liquid includes CNF (5(6)-carboxyfluorescein), the sensing membrane includes a second pH sensing membrane, and the second pH sensing membrane is used to monitor the pH value; the sol-gel liquid is applied to the preset area of ​​the probe to form a sensing membrane, including: applying and covering the preset area with the sol-gel liquid doped with the CNF indicator by the immersion-pulling method to form a second pH sensing membrane for monitoring the pH value. By the immersion-pulling method, the preset area of ​​the probe is vertically immersed in the sol-gel liquid, which is doped with the CNF indicator. After immersion for a period of time, the preset area of ​​the probe is vertically pulled up from the sol-gel liquid at a low speed, and the sol-gel liquid attached to the preset area of ​​the probe covers the preset area. The sensing membrane formed is the second pH sensing membrane, which can be used to monitor the pH value. The second pH sensing membrane can also be called a fluorescence sensing membrane. In the detection process of the probe formed with the second pH value sensing membrane, the second pH value sensing membrane is immersed in the solution to be tested. Since the CNF indicator has an isosbestic point that is independent of the excitation light near the wavelength of 640nm, the fluorescent isomers of CNF are on both sides of the isosbestic point. For example, an LED light source with an emission light beam wavelength of 514.5nm is used. The LED light source is incident on the second pH value sensing membrane and is induced to emit fluorescence by the second pH value sensing membrane. The fluorescence intensity of the acid isomer of the CNF indicator at a wavelength of about 595nm decreases as the pH value increases, and the fluorescence intensity of the base isomer at about 660nm increases as the pH value increases. The calibration curve of the ratio of the fluorescence intensity at 660nm to 595nm normalized by the isosbestic point and the pH value can be obtained. Among them, I 660 I represents the fluorescence intensity of the system at 660 nm after normalization of the isosbestic point when the CNF indicator is present. 595 It represents the system fluorescence intensity at 595nm normalized to the isosbestic point when the CNF indicator exists. a and b are the calibration curve fitting coefficients of fluorescence intensity and pH value. a and b are constant values, thereby determining the pH value of the solution to be tested.

[0070] Figure 2 is a schematic flow chart of another method for preparing a probe provided in an embodiment of the present invention, Figure 3 is a schematic diagram of the structure of a probe provided by an embodiment of the present invention, and this embodiment is optimized on the basis of the above embodiment. Optionally, the probe includes a multimode optical fiber, the multimode optical fiber includes a core, a cladding and a coating layer, the cladding surrounds the core, and the coating layer surrounds the cladding;

[0071] Before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes:

[0072] At a preset area, the coating and cladding of the multimode optical fiber are removed until the fiber core is exposed;

[0073] The sol-gel liquid is applied to a preset area of ​​the probe to form a sensing film, including:

[0074] The sol-gel solution is applied to the exposed fiber core to form a sensing film.

[0075] For details not yet provided in this embodiment, please refer to the above embodiments. Figure 2 As shown, the preparation method comprises:

[0076] S210, preparing a sol-gel solution, wherein the sol-gel solution is doped with an indicator.

[0077] S220, removing the coating layer and cladding of the multimode optical fiber at a preset area until the fiber core is exposed.

[0078] Optionally, the coating and cladding of the multimode optical fiber are removed at a preset area until the fiber core is exposed, including: using a stripping machine to strip off the coating at the preset area; using an etching mixture to etch off the cladding at the preset area until the fiber core is exposed; wherein the etching mixture is a mixture of hydrofluoric acid, acetic acid and deionized water in a ratio of 1:4:5.

[0079] Specifically, Figure 4 is a schematic cross-sectional view of a multimode optical fiber provided by an embodiment of the present invention, such as Figure 3 and Figure 4 As shown, the probe includes a multimode optical fiber 10. For example, the multimode optical fiber 10 can be made of quartz optical fiber. The optical fiber diameter of the multimode optical fiber 10 can be 200-600 μm, and the refractive index of the multimode optical fiber 10 can be 1.45. The multimode optical fiber 10 includes a core 11, a cladding 12 and a coating 13. The cladding 12 surrounds the core 11, and the coating 13 surrounds the cladding 12. It should be noted that Figure 4 The multimode optical fiber 10 shown is a schematic cross-sectional view of an unprocessed optical fiber, and is not a schematic cross-sectional view of a probe after being manufactured into a probe.

[0080] The probe includes a preset area 20, and a sensing film 21 can be formed at the preset area 20. It should be noted that the selection of the preset area 20 is related to the size of the probe, the detection type and the scene. For example, the preset area 20 can be located in the middle of the probe, the preset area 20 can also be located at the end of the probe, or the preset area 20 can also be located at any position of the probe. The area outside the preset area 20 on the probe can be protected and fixed with paraffin to avoid the area outside the preset area 20 on the probe from being damaged and the situation where the preset area 20 cannot be distinguished and confirmed. At the preset area 20 of the probe, a stripping machine can be used to strip off the coating 13. For example, the stripping machine can be an electric stripper, a wire stripper, etc. The stripping machine will not scratch the surface of the multimode optical fiber 10 and has little effect on the multimode optical fiber 10. In addition, the coating 13 can also be stripped off with manual tools such as a wire brush and sandpaper, and the coating 13 can also be softened and removed with a chemical solution. The removal of the coating 13 can reduce the outer diameter of the probe on the one hand, and facilitate the subsequent etching process of the cladding 12 on the other hand.

[0081] Afterwards, the preset area 20 of the probe is immersed in the etching mixture, and the cladding 12 is etched away by the etching mixture until the core 11 is exposed, and then the probe is taken out from the etching mixture. The etching mixture is a mixture of hydrofluoric acid, acetic acid and deionized water in a ratio of 1:4:5, and the reaction rate of the cladding 12 in the etching mixture is 2.5 μm / h. During the etching process using the etching mixture, as the etching progresses, the concentration of the etching mixture should be appropriately reduced to reduce the reaction rate of the cladding 12 in the etching mixture, so as to accurately control the etching process and avoid over-etching the core 11. In addition, after the etching process using the etching mixture is completed, the surface of the core 11 can be repeatedly cleaned with deionized water to avoid the influence of the residual etching mixture on the core 11.

[0082] S230, applying the sol-gel solution onto the exposed fiber core to form a sensing film.

[0083] Specifically, continue to refer to Figure 3 and Figure 4 , the sol-gel liquid is applied to the exposed core 11 to ensure that the surface of the core 11 is wrapped in the sol-gel liquid, which is beneficial to the subsequent formation of the sensing film 21 and the detection effect of data detection.

[0084] Continue to refer Figure 3 The probe can be prepared by the method for preparing the probe of the embodiment of the present invention.

[0085] Figure 5 is a schematic flow chart of another method for preparing a probe provided in an embodiment of the present invention, Figure 6is a schematic diagram of the structure of another probe provided by an embodiment of the present invention, and this embodiment is optimized on the basis of the above embodiment. Optionally, the probe includes a multimode optical fiber, the multimode optical fiber includes a core, a cladding and a coating layer, the cladding surrounds the core, and the coating layer surrounds the cladding;

[0086] Before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes:

[0087] At a preset area, the coating and cladding of the multimode optical fiber are removed until the fiber core is exposed;

[0088] The sol-gel liquid is applied to a preset area of ​​the probe to form a sensing film, including:

[0089] The sol-gel solution is applied to the exposed fiber core to form a sensing film.

[0090] Further, the probe includes a plurality of preset areas;

[0091] At the preset area, remove the coating and cladding of the multimode optical fiber until the core is exposed, including:

[0092] At each preset area, the coating layer and the cladding layer of the multimode optical fiber are removed respectively until the fiber core is exposed in each preset area;

[0093] The sol-gel solution is applied to the exposed fiber core to form a sensing film, including:

[0094] Sol-gel liquid doped with different types of indicators is coated on the exposed fiber cores in each preset area to form a plurality of different types of sensing films.

[0095] For details not yet provided in this embodiment, please refer to the above embodiments. Figure 5 As shown, the preparation method comprises:

[0096] S310, preparing a sol-gel solution, wherein the sol-gel solution is doped with an indicator.

[0097] S320, at each preset area, respectively remove the coating layer and the cladding layer of the multimode optical fiber until the fiber core is exposed in each preset area.

[0098] Specifically, continue to refer to Figure 4 and Figure 6The probe includes a plurality of preset areas 20, each of which is used to form a different type of sensing film 21 for different data detection processes. The coating layer 13 of each preset area 20 can be removed by a stripping machine. The area outside each preset area 20 on the probe can be protected and fixed by paraffin wax. Each preset area 20 on the probe can be immersed in an etching mixture, and the cladding 12 of each preset area 20 can be etched away by the etching mixture until each preset area 20 exposes the core 11, and then the probe is taken out from the etching mixture.

[0099] S330, applying sol-gel liquid doped with different types of indicators to the exposed fiber cores in each preset area to form a plurality of different types of sensing films.

[0100] Specifically, continue to refer to Figure 4 and Figure 6 , by the immersion and pulling method, the sol-gel liquid doped with different types of indicators is respectively applied to the exposed fiber core 11 of each preset area 20 to ensure that the surface of the fiber core 11 is wrapped in the sol-gel liquid, which is conducive to the subsequent formation of the sensing membrane 21 and the detection effect of data detection. Exemplarily, the probe may include two preset areas 20, one of which is used to form a dissolved oxygen sensing membrane, which is used to monitor the dissolved oxygen content, and the other preset area 20 is used to form a first pH value sensing membrane, which is used to monitor the pH value. In the detection process of the probe, the dissolved oxygen content and the pH value can be detected at the same time, which can shorten the detection time, improve the detection efficiency, and simplify the detection operation.

[0101] Continue to refer Figure 6 The probe can be prepared by the method for preparing the probe of the embodiment of the present invention.

[0102] Figure 7 is a schematic flow chart of another method for preparing a probe provided in an embodiment of the present invention, Figure 8 is a schematic diagram of the structure of another probe provided by an embodiment of the present invention, and this embodiment is optimized on the basis of the above embodiment. Optionally, the probe includes an optical fiber bundle and a capillary end, the capillary end includes a first end and a second end opposite to each other, the first end is an open end, and the first end is used to insert the optical fiber bundle and be fixedly connected to the optical fiber bundle, and the second end is a closed spherical convex bottom structure;

[0103] Before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes:

[0104] Insert one end of the optical fiber bundle into the first end, and glue and fix the first end to one end of the optical fiber bundle by using optical fiber matching liquid or UV acrylic glue;

[0105] The sol-gel liquid is applied to a preset area of ​​the probe to form a sensing film, including:

[0106] The sol-gel solution is coated on the surface of the capillary end to form a sensing film.

[0107] For details not yet provided in this embodiment, please refer to the above embodiments. Figure 7 As shown, the preparation method comprises:

[0108] S410, preparing a sol-gel solution, wherein the sol-gel solution is doped with an indicator.

[0109] S420, inserting one end of the optical fiber bundle into the first end, and gluing and fixing the first end and one end of the optical fiber bundle by using optical fiber matching liquid or UV acrylic glue.

[0110] Specifically, Fig. 9 is a cross-sectional schematic diagram of an optical fiber bundle provided by an embodiment of the present invention, such as Figure 8 and Fig. 9 As shown, the probe includes an optical fiber bundle 30 and a capillary end 40. The optical fiber bundle 30 includes at least two fiber cores 11, a cladding 12 and a coating 13. A portion of the at least two fiber cores 11 is used for emitting light, and the remaining portion of the fiber cores 11 is used for receiving light. In order to determine the incidence and emission of the light beam, among the at least two fiber cores 11, a portion of the at least two fiber cores 11 is used for emitting light, and the remaining portion of the fiber cores 11 is used for receiving light. Exemplarily, if the optical fiber bundle 30 includes seven fiber cores 11, the optical fiber bundle 30 can be a one-core light-emitting and six-core light-receiving structure (or a six-core light-emitting and one-core light-receiving structure), a two-core light-emitting and five-core light-receiving structure (or a five-core light-emitting and two-core light-receiving structure), and a three-core light-emitting and four-core light-receiving structure (or a four-core light-emitting and three-core light-receiving structure). It should be noted that Fig. 9 The optical fiber bundle 30 shown is a schematic cross-sectional view of an unprocessed optical fiber, and is not a schematic cross-sectional view of a probe after being manufactured into a probe.

[0111] The capillary end 40 can be made of transparent plastic or glass (such as high borosilicate glass or inorganic transparent glass such as quartz, etc.), and the inner diameter range of the capillary end 40 can be 0.2-1.2mm, and the outer diameter range can be 1-2mm. The capillary end 40 includes a first end 41 and a second end 42 opposite to each other. The first end 41 is an open end, and the first end 41 can be used to insert the optical fiber bundle 30 and be fixedly connected to the optical fiber bundle 30. The second end 42 is a closed spherical convex bottom structure, that is, the capillary end 40 is a single-sealed structure. In addition, the capillary end 40 is a convex bottom structure, and the shape of the convex bottom structure can be hemispherical, ellipsoidal, bullet-shaped or triangular. For example, the ratio of the spherical diameter height of the convex bottom structure to the outer diameter of the capillary end 40 can be 1 / 4-1. First, one end of the optical fiber bundle 30 is inserted into the first end 41, and then the first end 41 can be glued to one end of the optical fiber bundle 30 through optical fiber matching liquid or UV acrylic glue to achieve a fixed connection between the first end 41 and one end of the optical fiber bundle 30, and the end face of the optical fiber bundle 30 should be as close as possible to the inner cavity of the capillary end 40. Exemplarily, the refractive index of the optical fiber matching liquid or UV acrylic glue can be 1.40.

[0112] S430, applying the sol-gel solution onto the surface of the capillary end to form a sensing film.

[0113] Specifically, continue to refer to Figure 8 The sol-gel liquid is applied to the surface of the capillary end 40 by the immersion pulling method to ensure that the surface of the capillary end 40 is wrapped in the sol-gel liquid, which is beneficial to the subsequent formation of the sensing film 21 and the detection effect of the data detection.

[0114] Continue to refer Figure 8 The probe can be prepared by the method for preparing the probe of the embodiment of the present invention.

[0115] Optionally, before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes: performing a hydrophilic surface treatment on the capillary end.

[0116] Specifically, the capillary end is subjected to hydrophilic surface treatment by first soaking the capillary end in concentrated nitric acid solution for 1-24 hours, then ultrasonically cleaning the capillary end with ultrapure water, ethanol and isopropanol for multiple times, drying with nitrogen, drying in an oven for 2-10 hours, and finally placing in a dryer for standby use. The hydrophilic surface treatment is conducive to the subsequent coating of the sol-gel solution on the capillary end.

[0117] Optionally, the optical fiber bundle includes at least two fiber cores, a cladding and a coating, the cladding surrounds each fiber core, and the coating surrounds the cladding; before gluing and fixing the first end to one end of the optical fiber bundle by optical fiber matching liquid or UV acrylic glue, it also includes: removing at least part of the coating of the optical fiber bundle.

[0118] Specifically, a stripping machine can be used to remove at least part of the coating of the optical fiber bundle, and the coating at one end of the optical fiber bundle fixedly glued to the first end can be mainly removed. Removal of the coating is conducive to reducing the outer diameter of the probe and facilitating the matching of the optical fiber bundle with the capillary end. Exemplarily, the thickness of the removed coating can be 20-100 mm.

[0119] Optionally, the probe also includes a plastic tube, and the first end is fixedly connected to one end of the plastic tube; before the first end is glued and fixed to one end of the optical fiber bundle by optical fiber matching liquid or UV acrylic glue, it also includes: polishing the first end to make the first end rough and have a step shape; gluing and fixing the first end to one end of the plastic tube by UV glue or epoxy glue.

[0120] Specifically, the probe also includes a plastic tube, and the plastic tube can be made of materials such as polytetrafluoroethylene tube (PTFE), polyimide (PI) tube, polyetheretherketone (PEEK) tube, etc. The plastic tube can be a soft plastic tube or a hard plastic tube. The length of the plastic tube can be adjusted according to the actual use situation. For example, the length of the plastic tube can be 50-200cm. The first end of the capillary end can be first glued and fixed with one end of the plastic tube by UV glue or epoxy glue, and then the optical fiber bundle is inserted into the plastic tube, and the optical fiber matching liquid or UV acrylic glue is applied to the first end of the capillary end by a small syringe to achieve the gluing and fixing of the first end of the capillary end and one end of the optical fiber bundle. Among them, UV glue or epoxy glue has the advantages of good biocompatibility and low viscosity. Since the first end of the capillary end may not completely match one end of the plastic tube, the first end of the capillary end may be polished to make the first end rough and have a step shape. For example, the first end of the capillary end is cut out to a length of 5-20 mm from the sensing film on the capillary end, and the surface of the capillary end 2-10 mm away from the cut is polished to polish out a rough surface step with a height of 20-800 μm.

[0121] Based on the same inventive concept, an embodiment of the present invention further provides an optical sensing system. Fig.10 , Fig.11 and Fig.12 is a schematic diagram of the structure of three optical sensing systems provided by embodiments of the present invention, such as Fig.10 , Fig.11 and Fig.12As shown, the optical sensing system includes a light source module 50, a detection module 60 and a probe 1 as provided in an embodiment of the present invention, wherein the probe 1 includes a sensing film 21; the light source module 50 is connected to the end of the probe 1 away from the sensing film 21, and the light source module 50 is used to emit a light beam to the probe 1; the detection module 60 is connected to the end of the probe 1 away from the sensing film 21, and the detection module 60 is used to receive the light beam reflected by the probe 1. Further, it also includes an optical fiber interface 70 and a connecting optical fiber 80; the light source module 50 is connected to the end of the probe 1 away from the sensing film 21 through the optical fiber interface 70 and the connecting optical fiber 80 in turn; the detection module 60 is connected to the end of the probe 1 away from the sensing film 21 through the optical fiber interface 70 and the connecting optical fiber 80 in turn.

[0122] Specifically, the optical sensing system includes a light source module 50, a detection module 60, a probe 1, an optical fiber interface 70 and a connecting optical fiber 80, and the probe 1 includes a sensing film 21. The light source module 50 is connected to the end of the probe 1 away from the sensing film 21 through the optical fiber interface 70 and the connecting optical fiber 80 in turn, and the light source module 50 is used to emit a light beam to the probe 1. Exemplarily, the light source module 50 may include multiple light sources, and the light source module 50 may emit narrowband light source signals and / or broadband light source signals. The detection module 60 is connected to the end of the probe 1 away from the sensing film 21 through the optical fiber interface 70 and the connecting optical fiber 80 in turn, and the detection module 60 is used to receive the light beam reflected by the probe 1. Optionally, the detection module 60 includes a spectrometer, which can present the information of the received light beam in the form of a spectrum. Exemplarily, the optical fiber interface 70 can be an SMA905 connector, an MPO connector or a duplex LC quick plug, and the interface type can be an FC, LC, SC or SMA interface, etc. Fig.10 and Fig.11 As shown, the circulator 71 is also included. The circulator 71 is located at one end of the probe 1 away from the sensing film 21. The circulator 71 can be connected to the optical fiber interface 70 through the connecting optical fiber 80, or the circulator 71 can be connected to the optical fiber interface 70 through the optical fiber bundle in the probe 1. Fig.10 The probe 1 shown includes a sensing film 21. The sensing film 21 on the probe 1 is located on the side wall of one end of the probe 1. Fig.11 The probe 1 shown includes two sensing films 21. The sensing films 21 on the probe 1 are respectively located on the side wall of one end of the probe 1. Fig.12 The probe 1 shown comprises a sensing film 21. The sensing film 21 on the probe 1 is located on the surface of the capillary end.

[0123] Optionally, continue to refer to Fig.10 , Fig.11 and Fig.12 The optical sensing system further includes a computer module 90 , which is electrically connected to the detection module 60 , and is used to analyze the detection result of the detection module 60 .

[0124] Optionally, Fig.13 is a schematic diagram of the structure of a light source module provided by an embodiment of the present invention, such as Fig.10 , Fig.11 , Fig.12 and Fig.13 As shown, the light source module 50 includes multiple light sources 51 and a coupling lens group 52; each light source 51 is lit at the same time; the light source 51 is used to emit an LED light beam; the coupling lens group 52 is located on the light path of the emitted light of the light source 51, and the coupling lens group 52 is used to couple the LED light beams emitted by each light source 51 into a composite light beam.

[0125] Specifically, the light source module 50 includes a plurality of light sources 51 and a coupling lens group 52. Exemplarily, the light source module 50 includes two light sources 51 (exemplarily, the two light sources 51 are respectively a first light source 511 and a second light source 512) and a coupling lens group 52 (exemplarily, the coupling lens group 52 includes a first coupling lens 521 and a second coupling lens 522). Each light source 51 is lit at the same time, and the coupling lens group 52 is located on the light path of the output light of the light source 51. The coupling lens group 52 can couple the LED light beams emitted by each light source 51 into a composite light beam. The coupling lens group 52 plays the role of light uniformity and focusing to ensure that the light beams emitted by the plurality of light sources 51 can be evenly coupled into the optical fiber interface 70. It should be noted that the number of light sources 51 is set the same as the number of sensing films on the probe. The LED light beams emitted by the light sources 51 of different wavelength ranges are incident on different sensing films on the probe, and the generated fluorescence intensity and material detection effect are different. Exemplarily, the first light source 511 may be a wide-spectrum LED (white light LED, with a wavelength range of 400-700nm), and the sensing film corresponding to the first light source 511 may be a first pH value sensing film. The first light source 511 may also be a single-wavelength LED (with a wavelength of 514.5nm), and the sensing film corresponding to the first light source 511 may be a second pH value sensing film. The second light source 512 may be a single-wavelength LED (with a wavelength of 450nm), and the sensing film corresponding to the second light source 512 may be a dissolved oxygen sensing film. The light source module 50 of the embodiment of the present invention may be applied to Fig.10 The optical sensing system shown performs two different data detection processes simultaneously.

[0126] Optionally, Fig.14 is a schematic diagram of the structure of another light source module provided by an embodiment of the present invention. Fig.10 , Fig.11 , Fig.12 and Fig.14As shown, the light source module 50 includes multiple light sources 51 and multiple plano-convex lenses 53, the number of light sources 51 and the plano-convex lenses 53 are the same and correspond one to one; each light source 51 is lit in time or at the same time; the light source 51 is used to emit an LED light beam; the plano-convex lens 53 is located on the light path of the emitted light of the light source 51, and the plano-convex lens 53 is used to collimate the LED light beam emitted by the light source 51 into a beam of parallel light.

[0127] Specifically, the light source module 50 includes a plurality of light sources 51 and a plurality of plano-convex lenses 53, and the number of light sources 51 and the plano-convex lenses 53 are the same and correspond one to one. Exemplarily, the light source module 50 includes two light sources 51 and two plano-convex lenses 53, and the two light sources 51 can emit LED light beams of different wavelength ranges, and the plano-convex lenses 53 are located on the light path of the emitted light of the light source 51, and the plano-convex lenses 53 can collimate the LED light beam emitted by the light source 51 into a beam of parallel light, and couple it into the optical fiber interface 70. It should be noted that the number of light sources 51 is set to be the same as the number of sensing films on the probe, and the LED light beams emitted by the light sources 51 of different wavelength ranges are incident on different sensing films on the probe, and the generated fluorescence intensity and material detection effect are different. Through circuit control, each light source 51 can be lit up at different times. Then, the optical sensing system can be provided with a fiber optic interface 70 accordingly. The LED light beams emitted by each light source 51 are alternately coupled into the fiber optic interface 70. Multiple sensing films can be provided on the probe to avoid frequent replacement during the detection process. The optical sensing system can also be provided with multiple fiber optic interfaces 70 accordingly. The LED light beams emitted by each light source 51 are respectively coupled into a fiber optic interface 70. Each fiber optic interface 70 is connected to a probe provided with a different type of sensing film. Through circuit control, each light source 51 can be lit up at the same time. Then, the optical sensing system can be provided with a fiber optic interface 70 accordingly. The LED light beams emitted by each light source 51 are coupled into a composite light beam and then coupled into the fiber optic interface 70. Multiple sensing films are provided on the probe to perform two different data detection processes simultaneously.

[0128] Optionally, Fig.15 is a structural diagram of another light source module provided by an embodiment of the present invention, such as Fig.10 , Fig.11 , Fig.12 and Fig.15As shown, the light source module 50 includes multiple light sources 51, multiple plano-convex lenses 53, a dichroic mirror 54 and an aspheric lens 55. The number of light sources 51 and the plano-convex lenses 53 are the same and correspond to each other one by one. Each light source 51 is lit in time or at the same time. The light source 51 is used to emit an LED light beam. The plano-convex lens 53 is located on the light path of the emitted light of the light source 51, and the plano-convex lens 53 is used to collimate the LED light beam emitted by the light source 51 into a beam of parallel light. The dichroic mirror 54 is located on the light path of the emitted light of the plano-convex lens 53, and the dichroic mirror 54 is used to couple each parallel light into a beam of composite light. The aspheric lens 55 is located on the light path of the emitted light of the dichroic mirror 54, and the aspheric lens 55 is used to collimate the composite light into a beam of parallel composite light.

[0129] Specifically, the light source module 50 includes a plurality of light sources 51, a plurality of plano-convex lenses 53, a dichroic mirror 54 and an aspheric lens 55. The number of light sources 51 and the plano-convex lenses 53 is the same and corresponds to each other. Exemplarily, the light source module 50 includes two light sources 51, two plano-convex lenses 53, a dichroic mirror 54 and an aspheric lens 55. The two light sources 51 can emit LED light beams of different wavelength ranges, and the light paths of the two light sources 51 are the same. The plano-convex lens 53 is located on the light path of the outgoing light of the light source 51, and the plano-convex lens 53 can collimate the LED light beam emitted by the light source 51 into a beam of parallel light. The dichroic mirror 54 is located on the light path of the outgoing light of the plano-convex lens 53, and the dichroic mirror 54 can couple each parallel light into a beam of composite light. The aspheric lens 55 is located on the light path of the outgoing light of the dichroic mirror 54, and the aspheric lens 55 can collimate the composite light into a beam of parallel composite light, and couple it into the optical fiber interface 70. It should be noted that the number of light sources 51 is set to be the same as the number of sensing films on the probe. The LED light beams emitted by the light sources 51 of different wavelength ranges are incident on different sensing films on the probe, and the generated fluorescence intensity and material detection effect are different. Through circuit control, each light source 51 can be lit up in time, and the optical sensing system can be set up with a fiber optic interface 70 accordingly. The LED light beams emitted by each light source 51 are alternately coupled into the fiber optic interface 70, and multiple sensing films can be set up on the probe to avoid frequent replacement during the detection process. Through circuit control, each light source 51 can be lit up at the same time, and the optical sensing system can be set up with a fiber optic interface 70 accordingly. The LED light beams emitted by each light source 51 are coupled into a composite light and then coupled into the fiber optic interface 70. Multiple sensing films are set up on the probe to perform two different data detection processes at the same time.

[0130] Note that the above are only preferred embodiments of the present invention and the technical principles used. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments, combinations and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in more detail through the above embodiments, the present invention is not limited to the above embodiments, and may include more other equivalent embodiments without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A method for preparing a probe, characterized in that: include: preparing a sol-gel solution, wherein the sol-gel solution is doped with an indicator; Applying the sol-gel solution on a preset area of ​​the probe to form a sensing film; The sensing membrane is used to monitor physiological index parameters.

2. The preparation method according to claim 1, characterized in that: The probe comprises a multimode optical fiber, wherein the multimode optical fiber comprises a core, a cladding and a coating layer, wherein the cladding surrounds the core, and the coating layer surrounds the cladding; Before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes: At the preset area, removing the coating layer and the cladding layer of the multimode optical fiber until the fiber core is exposed; The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising: The sol-gel solution is applied on the exposed core to form the sensing film.

3. The preparation method according to claim 2, characterized in that: The probe includes a plurality of the preset areas; At the preset area, removing the coating layer and the cladding of the multimode optical fiber until the core is exposed, comprising: At each of the preset areas, the coating layer and the cladding layer of the multimode optical fiber are removed respectively until the fiber core is exposed in each of the preset areas; Applying the sol-gel solution on the exposed core to form the sensing film comprises: The sol-gel solution doped with different types of the indicator is respectively coated on the exposed fiber cores in each of the preset areas to form a plurality of different types of the sensing films.

4. The preparation method according to claim 1, characterized in that: The probe comprises an optical fiber bundle and a capillary end, wherein the capillary end comprises a first end and a second end opposite to each other, wherein the first end is an open end and is used to insert the optical fiber bundle and be fixedly connected to the optical fiber bundle, and the second end is a closed spherical convex bottom structure; Before applying the sol-gel solution to a preset area of ​​the probe to form a sensing film, the method further includes: Inserting one end of the optical fiber bundle into the first end, and gluing and fixing the first end to one end of the optical fiber bundle by using optical fiber matching liquid or UV acrylic glue; The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising: The sol-gel solution is coated on the surface of the capillary end to form the sensing film.

5. The preparation method according to claim 1, characterized in that: The indicator doped in the sol-gel solution includes Ru(dpp)3Cl2 (4,7-diphenyl-1,10-phenanthroline ruthenium dichloride), and the sensing membrane includes a dissolved oxygen sensing membrane, and the dissolved oxygen sensing membrane is used to monitor the dissolved oxygen content; The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising: The sol-gel solution doped with the Ru(dpp)3Cl2 indicator is applied to and covers the preset area by an immersion-pulling method to form the dissolved oxygen sensing membrane for monitoring the dissolved oxygen content.

6. The preparation method according to claim 1, characterized in that: The indicator doped in the sol-gel solution includes BCG (bromocresol green) or CNF (5(6)-carboxyfluorescein), and the sensing membrane includes a first pH value sensing membrane or a second pH value sensing membrane, and the first pH value sensing membrane and the second pH value sensing membrane are used to monitor the pH value; The sol-gel solution is applied to a preset area of ​​the probe to form a sensing film, comprising: The sol-gel solution doped with the BCG indicator or the CNF indicator is applied to and covers the preset area by an immersion-pulling method to form the first pH-sensing membrane or the second pH-sensing membrane for monitoring pH value.

7. A probe, characterized in that: The probe is prepared by the method for preparing the probe according to any one of claims 1 to 6.

8. An optical sensing system, characterized in that: It comprises a light source module, a detection module and the probe as claimed in claim 7, wherein the probe comprises a sensing film; The light source module is connected to an end of the probe away from the sensing film, and the light source module is used to emit a light beam to the probe; The detection module is connected to an end of the probe away from the sensing film, and the detection module is used to receive the light beam reflected by the probe.

9. The optical sensing system according to claim 8, characterized in that: The light source module includes a plurality of light sources and a coupling lens group; each of the light sources is lit at the same time; The light source is used to emit an LED light beam; The coupling lens group is located on the optical path of the emitted light from the light source, and is used to couple the LED light beams emitted by each of the light sources into a composite light beam.

10. The optical sensing system according to claim 8, characterized in that: The light source module comprises a plurality of light sources, a plurality of plano-convex lenses, a dichroic mirror and an aspherical lens, wherein the number of the light sources and the plano-convex lenses is the same and corresponds one to one; each of the light sources is lit up in time or at the same time; The light source is used to emit an LED light beam; The plano-convex lens is located on the optical path of the outgoing light from the light source, and the plano-convex lens is used to collimate the LED light beam emitted by the light source into a beam of parallel light; The dichroic mirror is located on the outgoing light path of the plano-convex lens, and the dichroic mirror is used to couple the parallel lights into a composite light. The aspherical lens is located on the optical path of the outgoing light of the dichroic mirror, and the aspherical lens is used to collimate the composite light into a beam of parallel composite light.

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