Extraction method of sargassum pallidum extract, blue light protection effect composition containing sargassum pallidum extract and wash supplies containing sargassum pallidum extract

Abstract

The invention belongs to the technical field of natural product extraction and the field of daily chemicals. According to the composition, effective substances contained in sargassum pallidum are combined with fullerene to achieve an excellent blue light protection effect, and the composition can absorb blue light, resist oxidation, resist / scavenge free radicals, delay the aging speed of skin and relieve hyperpigmentation to achieve the blue light protection effect and reduce damage caused by blue light to the skin. The invention further provides the face cleaning composition with the blue light protection effect by reasonably constructing a composition system structure and limiting key parameters of raw materials.

Description

Technical Field

[0001] The invention belongs to the technical field of natural product extraction and the field of daily chemicals. Background Art

[0002] The wavelength range of the spectrum that the human eye can perceive is 380-780nm, which is also called the visible light band. Blue light usually refers to the closest violet light in the visible light, also known as HIGH ENERGY VISBLE LIGHT, or HEVlight for short. The wavelength range of the blue band of visible light is 380nm to 500nm, of which the wavelength range of high-energy blue light is 415nm to 455nm. It has relatively high energy light and strong penetration, and is harmful blue light. With the development of human society, blue light is ubiquitous, and is commonly found in the light projected by lighting fixtures and 3C electronic product screens in modern life. Solar radiation is the main source of blue light. In the past, people mainly focused on the damage of ultraviolet rays in sunlight to human skin, but the blue light area of ​​the spectrum has been largely ignored. Generally speaking, about 25% of sunlight is blue light, which is the shortest wavelength and highest energy band in the visible spectrum. It will reach deeper into the skin and has stronger penetration ability than ultraviolet rays. If the skin is exposed to blue light sources for a long time, the impact on the skin will be more lasting.

[0003] The harm of blue light to the skin is mainly manifested in three aspects: (a) causing cell apoptosis and damage. First, blue light acts on mitochondria, because mitochondria are the main site of oxidative stress and the main site of reactive oxygen free radicals (ROS) in cells, which can regulate cell apoptosis. Mitochondria absorb blue light (415nm~455nm) through the electron respiratory chain, undergo oxidation reactions, and induce the formation of ROS. ROS reduces the mitochondrial membrane potential, opens the mitochondrial permeability transition pore (MPTP), releases mitochondrial cytochrome C (CytC), and combines with apoptosis protein activating factor-1 (Apaf-1) to form the apoptosis complex Apaf-1-CytC; the apoptosis complex in the cytoplasmic matrix recruits the Caspase family, Caspase-9 self-cleaves and activates, activates Caspase-3 and Caspase-7, initiates a series of cascade reactions, and induces cell apoptosis. Second, blue light mediated by the fluorescent group A2E of lipofuscin can cause damage to cells. The intracellular metabolite lipofuscin is a marker of senescent cells. Its main fluorescent group N-retinylidene-N-retinolamine (A2E) is very sensitive to blue light. Under the stimulation of blue light, it can accelerate the production of ROS and activate the cell apoptosis pathway. Studies have shown that A2E is mainly present in lysosomes, with a small amount distributed on the mitochondrial membrane, and does not exist in other organelles. A2E mediates blue light and has two aspects of damage to cells. On the one hand, A2E is affected by blue light, which causes the lysosomal transmembrane electron gradient to be destroyed, causing lysosomal enzymes and ROS to be released into the cytoplasm, causing cell apoptosis; on the other hand, A2E on the mitochondrial membrane causes mitochondrial membrane abnormalities, prompting mitochondria to release death-promoting factors, Apaf, apoptosis-inducing factors (AIF), etc., and initiates the mitochondrial-mediated cell apoptosis process. (II) Causes skin photoaging. After blue light irradiates the skin, it induces an abnormal increase in ROS in skin cells, triggering a series of oxidative stress reactions and inflammatory reactions. Oxidative stress caused by blue light can further inhibit the TGF-β signaling pathway, activate the mitogen-activated protein kinase (MAPK) signaling pathway, and cause the downstream transcription factor c-Jun N-terminal kinase (JNK), which triggers calcium influx by increasing the expression of transient receptor potential vanilloid 4 (TRPV4) channel protein on the cell surface, inducing kinase (ERK) and p38 phosphorylation levels, activating transcription activator (AP-1), and thus inducing the expression of matrix metalloproteinases (MMPs). Among the abnormal expression of MMPs, MMP-1 can degrade the most important type I and type III collagen fibers in human skin, and MMP-3 can degrade type IV collagen fibers in the basement membrane, producing photoaging effects on the skin, causing wrinkles, sagging and other aging phenomena on the skin. (III) Causes skin pigmentation.Under the influence of a large number of reactive oxygen species, blue light can act on opsin 3 (OPN3), which is a member of the G protein-coupled receptor superfamily and exists in epidermal keratinocytes and melanocytes. It activates calcium / calmodulin-dependent protein kinase Ⅱ (CAMKⅡ) through OPN3, induces phosphorylation of cAMP-response element binding protein (CREB), microphthalmia-associated transcription factor (MITF), etc., and causes the expression of intracellular tyrosinase and dopachrome tautomerase, which leads to the formation of melanin and causes skin pigmentation. According to the above analysis, it can be concluded that the harm caused by blue light to the human body is no less than that caused by ultraviolet rays, so people should pay close attention to it. In this regard, based on the analysis of the hazards of blue light to the skin, the "anti-blue light" claimed in the present invention mainly achieves the purpose of reducing skin damage caused by blue light by absorbing blue light, anti-oxidation, resisting / scavenging free radicals to slow down the aging of the skin, and alleviating pigmentation, thereby playing a role in resisting the damage of blue light to the skin.

[0004] At present, there are few products on the market that protect the skin from blue light damage, and there are even fewer cleaning products with related effects. In the prior art, patent CN110433092A records a composition for repairing blue light damage, including cranberry extract, somnifera root extract, Mediterranean cypress leaf extract and wormwood extract. Patent CN110393676A records an anti-blue light composition, including: 10-50 parts of resveratrol, 10-50 parts of vitamin C, and 35-40 parts of vitamin E. Patent CN110575410A discloses a composition containing a protective blue light component, mainly containing marigold extract, camellia extract, milo extract, nicotinic acid, vitamin E, hyaluronic acid and other ingredients. Patent CN111494261A records a purple alfalfa extract seat anti-blue light component. Patent CN115025028A discloses a composition of carnosine, Dendrobium officinale stem extract and / or Egyptian blue water lily extract as a blue light protection component. However, in the existing research on blue light protection products, there is no record of using a combination of seaweed and fullerene as a blue light protection component. Cleaning products are used almost every day, so it is very meaningful to develop a skin care product that is suitable for daily cleaning and can also achieve blue light protection.

[0005] Seaweed was first recorded in Shennong's Herbal Classic. Seaweed contains some active substances with special physiological functions, with novel structure and significant physiological activity. It can be used as both medicine and food. Seaweed contains a large amount of chlorophyll, which can produce energy through photosynthesis through its own pigments. It has a high use value and is one of the important marine natural medicines. Common seaweed mainly includes three categories: green algae, red algae and brown algae, with a wide variety of genera and species. Among them, large-leaf seaweed is the dried algae of Sargassum pallidun (Turn.) C.Ag, a plant of the Sargassum family. It is wrinkled and curled, dark brown, and some are covered with white frost. It is 30 to 60 cm long. Its trunk is cylindrical with conical protrusions. The main branches grow from both sides of the trunk, and the side branches grow from the axils of the main branches and leaves, with short thorn-like protrusions. The primary leaves are lanceolate or obovate, 5-7cm long, about 1cm wide, entire or coarsely serrated; the secondary leaves are strip-shaped or lanceolate, with small branches of strip-shaped leaves in the axils. The air sacs are dark brown, spherical or oval, some with petioles, blunt tops, and some with short tips. It has rich chemical components, such as polysaccharides, lipids, proteins, minerals, vitamins, cellulose, polyphenols, seaweed glycoproteins, etc.

[0006] Fullerenes have been used in the cosmetics field because of their free radical scavenging and antioxidant effects. From the perspective of elemental composition, fullerenes are a type of hollow molecules composed of carbon atoms in the shape of ellipsoids, spheres or tubes. Like diamond and graphite, they are typical allotropes of carbon. From a structural point of view, fullerenes are very similar to graphite. They are both composed of cyclic molecules composed of carbon atoms. However, graphite has only a single six-membered ring, while the cyclic molecules of fullerenes are diverse, with both five-membered rings and six-membered rings, and seven-membered rings are occasionally seen. C60 is the most common fullerene, composed of 60 evenly distributed carbon atoms, with a unique football-shaped cage structure, so it is also called "football ene". C60 is a three-dimensional aromatic compound, and its formation mechanism is very similar to that of graphite: on the sp2 hybrid orbital, seemingly isolated carbon atoms will be connected to each other under the action of chemical bonds to form a spherical surface that is tightly connected to each other. This football-like cage structure is very stable and exhibits a high degree of symmetry and excellent physical and chemical properties. Summary of the invention

[0007] The technical problem to be solved by the present invention is to provide an extraction method of a large leaf seaweed extract. The present invention also provides a blue light protection efficacy composition of a large leaf seaweed extract and a fullerene stock solution, which can absorb blue light, resist oxidation, resist / scavenger free radicals, delay the aging of the skin, relieve pigmentation, play a blue light protection effect, and reduce the damage caused by blue light to the skin. The present invention further provides a facial cleansing composition with blue light protection efficacy by rationally constructing a composition system architecture and limiting key parameters of raw materials.

[0008] The technical solution adopted by the present invention to solve the above problems is as follows:

[0009] A method for extracting a macrophylla extract comprises the following steps:

[0010] (1) Impurity removal: Weigh an appropriate amount of large-leaf seaweed, remove impurities, wash it with deionized water until it is clean, then add a certain amount of deionized water, stir it thoroughly, and soak it at room temperature for 1-2 hours;

[0011] (2) Cell extraction: Take a suitable container and fully mash the soaked Zona algae, filter it with a 100-200 mesh filter cloth, repeat the above steps until the color of the filtrate becomes lighter, collect the filtrate, centrifuge for 3-5 minutes, repeat 3-5 times, remove the upper clear liquid, and collect the Zona algae cell fluid at the lower layer;

[0012] (3) Cell wall disruption: Add the Zona algae cell solution obtained in step (2) into a high-pressure cell disruptor, introduce carbon dioxide at 0.15-0.3 MPa, adjust the disruption time to 30-40 min, take samples 3-4 times in between, and stop the high-pressure treatment when a large amount of Zona algae cell walls are observed under a microscope to obtain a Zona algae crude solution;

[0013] (4) extracting essence: placing the macroalgae crude liquid treated in step (3) in an extraction tank for a first supercritical extraction, the extraction time is 100-120 min, the temperature is set to 30° C.-40° C., the extraction pressure is 30-40 MPa, and the flow rate of carbon dioxide is 600 kg / h, to obtain an extract A1 and a residue B1; the residue B1 is subjected to a second supercritical extraction, the extraction time is 60-80 min, the temperature is set to 30° C.-40° C., the extraction pressure is 30-40 MPa, and the flow rate of carbon dioxide is 600 kg / h, to obtain an extract A2;

[0014] (5) The extracts A1 and A2 are mixed evenly to obtain a macrophylla extract.

[0015] A proper amount of preservatives can also be added to the extract to obtain an essence of the large-leaf seaweed extract that can be stably stored and used for a long time.

[0016] A blue light protection composition comprises a large leaf seaweed extract and a fullerene stock solution, wherein the weight ratio of the two is 0.1-15:0.0001-0.01. The weight ratio is further preferably 3-15:0.0005-0.001, and more preferably 4000:1. The large leaf seaweed extract can be the large leaf seaweed extract prepared by the extraction method disclosed in the present invention, or other commercially available large leaf seaweed extracts can be used, and the fullerene stock solution can be a composition treated with a water-soluble polymer.

[0017] The above-mentioned blue light protection efficacy composition is added to toiletries at a mass percentage of 0.1001-15.01% to bring about blue light protection efficacy. The above-mentioned blue light protection efficacy composition can be applied to skin care or cleaning cosmetics, and the cosmetic dosage form can be one of a solution, a suspension, an emulsion, a paste, and a solid. Among them, the mass percentage of the large leaf seaweed extract in the toiletries is preferably 3%.

[0018] For example, a facial cleansing product contains the following components in percentage by weight:

[0019] (A) anti-blue light efficacy composition 0.1001-15.01%;

[0020] (B) surfactant 12-18%;

[0021] (C) 3-4% thickening stabilizer;

[0022] (D) pearlescent agent 2-3%;

[0023] (E) skin conditioning agent 0.5-1%; and the balance

[0024] (F) Polyol.

[0025] Among them, the surfactant is one or more of sodium cocoyl glycinate, potassium cocoyl glycinate, sodium lauroyl sarcosinate, cocamidopropyl betaine, sodium cocoyl glutamate, decyl glucoside, sodium cocoyl methyl taurate taurate, disodium lauroamphodiacetate, and sodium lauroamphoacetate, preferably sodium cocoyl glycinate and disodium lauroamphodiacetate.

[0026] Among them, the thickening stabilizer is a combination of one or more of hydroxypropyl starch sodium phosphate, hydroxypropyl starch and acrylic acid (ester) copolymer modified corn starch, which plays a role in stabilizing the system and improving the rheological properties of the paste, and is preferably hydroxypropyl starch sodium phosphate.

[0027] Wherein, the pearlescent agent is one or more of ethylene glycol distearate and ethylene glycol stearate.

[0028] The skin conditioning agent is one or more of sugar isomers, betaine, sodium PCA, niacinamide, and vitamin B6, which act as moisturizing factors to play a role in moisturizing and nourishing.

[0029] The polyol is used as a moisturizer and solvent, preferably one or more of glycerol, propylene glycol or 1,3-propylene glycol and butylene glycol, which can play a good moisturizing role and has a significant moisturizing effect on the skin. At the same time, it increases the solubility of other components in the system and makes the appearance of the paste more beautiful. The polyol includes propylene glycol or 1,3-propylene glycol and one or more of butylene glycol and glycerol, preferably glycerol.

[0030] In addition to the above-mentioned essential ingredients, the present invention may also contain at least one of the following components:

[0031] Preservatives: 0.01-1%;

[0032] pH adjuster: 0.001-2%

[0033] Fragrance agent: 0.01-3%;

[0034] The preparation method of the facial cleansing composition having blue light protection effect is as follows:

[0035] S1. Disperse hydroxypropyl sodium starch phosphate completely with glycerol, add surfactant, solid preservative and pearlescent agent, raise the temperature to 75-85°C, and keep warm for 28-38 minutes.

[0036] S2. After the insulation is completed, add citric acid into the system and adjust the pH to between 6.4 and 7.2.

[0037] S3. Start cooling down, and control the cooling speed to drop by 1°C every two minutes.

[0038] S4. When the temperature drops to 40-45°C, add the essence of the large-leaf kelp extract, fullerene stock solution, liquid preservative and skin conditioner, etc.

[0039] S5. Cool down to 35-40℃, the system starts to form a paste, adjust the stirring speed, stir until the paste is smooth and not rough, and proceed to the next step.

[0040] S6. Add the flavoring agent into the system and stir evenly.

[0041] S7. Re-test the pH value and adjust it to 6.2-7.0.

[0042] S8. After the viscosity test is qualified, it is completed.

[0043] The present invention has the following beneficial effects:

[0044] 1. The present invention adopts cell wall breaking-supercritical fluid extraction to prepare the extract of giant seaweed. The biggest advantage of cell wall breaking extraction is that the extraction temperature is not high, the extraction rate is high, the product is easy to purify, and it is green and environmentally friendly, which can effectively protect the giant seaweed rich in various chemical components; while the supercritical fluid extraction method has the dual characteristics of liquid and gas at the same time, its diffusion coefficient and viscosity are close to gas, while the molecular density is greatly increased, hundreds of times larger than gas, almost close to liquid, the increase in density increases the intermolecular interaction force, and at the same time accelerates the movement rate of molecules, and also increases the collision frequency between molecules, so that the molecules reach an activated state, and the solubility of compounds is enhanced, so the solubility of supercritical fluid is similar to that of liquid, but the extraction rate is more environmentally friendly and efficient than that of liquid solvent extraction. The present invention adopts cell wall breaking method combined with supercritical fluid extraction method, and wonderfully achieves the extraction effect of 1+1>2, so that the effective ingredients in giant seaweed can be brought into play to the greatest extent. If traditional single methods such as hot reflux method and acid extraction method are adopted, not only will the effective active substances of giant seaweed be destroyed during the extraction process, but the extraction efficiency is low.

[0045] 2. According to the hazards of blue light causing skin cell apoptosis and damage, skin photoaging and skin pigmentation, the present invention uses large leaf seaweed extract compounded with fullerene as a blue light protection efficacy composition. Large leaf seaweed extract is rich in chlorophyll, and the essence prepared by it has the function of absorbing blue light, directly reducing the penetration of blue light into the skin, and weakening the harmful effect of blue light on the skin; large leaf seaweed extract is rich in polysaccharides, among which the polysaccharides DEI and DEII connected by β-configuration pyranosidic bonds of monosaccharides can inhibit oxidative stress by activating the TGF-β pathway, reduce MMPs degradation of skin collagen, and delay skin aging, thereby preventing and treating blue light-induced skin photoaging; seaweed polyphenols can inhibit the phosphorylation of CREB and MITF, inhibit the effect of melanocyte stimulating hormone in promoting melanogenesis, reduce the synthesis of melanin in cells, and thus reduce pigmentation. Fullerene has two functions in the blue light protection efficacy composition. First, fullerene has a strong ability to capture free radical molecules. Free radicals free around cells will be captured by fullerene in large quantities and react with them to reduce the amount of free radicals in the biological medium. When the adsorption amount reaches an even number, the free radicals on the surface of fullerene are coupled with each other and quenched, and fullerene returns to the state before adsorption, and its biological activity is not reduced. Therefore, the collagen fibers of the skin are prevented from being lost due to decomposition, the activity of elastic fibers is maintained, and the good effects of anti-wrinkle and skin tightening are achieved, and skin aging is effectively slowed down and delayed. Second, under the action of fullerene, starting from the source of photoaging, by enhancing the action of superoxide dismutase in skin cells, a large amount of active oxygen components are removed, and the accumulation of active oxygen in skin tissue is reduced, thereby reducing the damage of active oxygen to the skin. In addition, the signal transmission process that stimulates skin tissue is blocked, thereby cutting off the formation pathway of melanin and reducing the deposition of melanin in skin tissue. The two work together to achieve the effect of 1+1>2. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 The blue light absorption spectra of the compositions with different ratios are tested. DETAILED DESCRIPTION

[0047] The features and performance of the present invention are further described in detail below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments, and are not limitations of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] Unless otherwise specified, the test materials, reagents, methods and equipment used in the following examples are conventional test materials, reagents, methods and equipment in the technical field; unless otherwise specified, the test materials and reagents used in the following examples can be obtained from commercial channels.

[0049] (I) Blue light absorption ability test

[0050] Test 1: Take the prepared large leaf seaweed extract essence and dilute it with distilled water to obtain large leaf seaweed extract aqueous solutions with mass fractions of 15%, 7%, 5%, 3% and 0.1%, respectively, and mark them as sample 1, sample 2, sample 3, sample 4, and sample 5. Take 80ml of sample 1, sample 2, sample 3, sample 4, and sample 5 respectively and put them in five plastic bottles with an inner diameter of about 4cm. Place the anti-blue light test card at a distance of about 5cm from the plastic bottle. On the other side of the plastic bottle, at a distance of 4-5cm from the bottle, use a blue light pen to irradiate to test the effect of large leaf seaweed extract essence on absorbing blue light. Take 80ml of distilled water and put it in a plastic bottle with an inner diameter of about 4cm, marked as sample 6, and operate in the same way as a control. The transmittance set in this experiment is 0-10, which indicates the degree of transmittance. The higher the transmittance, the worse the blue light absorption effect. The test results are shown in Table 1. According to the test results, the blue light absorption effect is best when the mass fraction of the giant seaweed extract aqueous solution is above 3%. Considering the production cost, the preferred solution in this experiment is the giant seaweed extract aqueous solution with a mass fraction of 3%.

[0051]

[0052] Test 2: According to Test 1, a 3% aqueous solution of a large leaf seaweed extract was selected, and the large leaf seaweed extract essence and the fullerene stock solution were mixed in a conventional manner to prepare a blue light protection efficacy composition; the composition was mixed at 3000:1, 4000:1, and 6000:1 to prepare a blue light protection efficacy composition, which were marked as sample A, sample B, and sample C, respectively, and a blue light spectrum was scanned to record the absorption value (absorbance A) in the 380nm to 500nm band. The test results are as follows: Figure 1 According to the test results, the applicant unexpectedly discovered that the blue light protection composition sample B prepared by mixing the macrophylla extract essence and the fullerene stock solution in a ratio of 4000:1 has a better blue light absorption effect in the 400nm-460nm band, while the high-energy blue light band with the strongest damage to the skin ranges from 415nm to 455nm, so the mixing ratio of the composition prepared by sample B is the best combination to achieve the effect of this experiment.

[0053] (II) Consumer Testing

[0054] The best combination for absorbing blue light obtained from the above experiments was applied to facial cleansing products, and preferred embodiments and comparative examples were selected. The following Table 2 shows preferred embodiments 1-3 and 3 comparative examples.

[0055] Table 2: Examples and Comparative Examples

[0056]

[0057] The above embodiments and comparative examples were evaluated by instrument efficacy evaluation and human sensory evaluation. The above embodiments 1-3 and comparative examples 1-3 were used for efficacy evaluation and human sensory experience evaluation, respectively. The change rates of the embodiments and comparative examples before and after use are the average results shown in Table 3.

[0058] Test description:

[0059] 1) Test equipment

[0060] Delfin ElastiMeter: It uses contact and pressure measurement. When the probe is in full contact with the skin, the pressure head will produce a constant deformation. By measuring the strength of the skin's resistance to deformation, the instantaneous elasticity of the skin is measured. The larger the ISE value, the better the instantaneous elasticity of the skin.

[0061] VISIA Facial Image Analyzer: Using advanced optical imaging, RBX and software technology, it has three built-in lights: standard light, polarized light, and UV (ultraviolet light), of which UV light can penetrate deep into the skin. It can instantly measure and analyze wrinkles, texture, skin quality, pores, pigment, red blood, oil distribution, and subcutaneous blood vessels and pigments under ultraviolet light.

[0062] Skin color measuring instrument (Delfin SkinColorCatch): detects skin color ITA° and melanin index MI. The smaller the MI value of the test area, the whiter the skin color, and vice versa. The larger the ITA° value, the lighter the skin color, and vice versa.

[0063] 2) Test environment: temperature 21±1℃, relative humidity 50%±10%RH

[0064] 3) Skin instant elasticity ISE: 60 subjects aged 30 to 65 years old were selected, regardless of gender. Two test sites of the subjects were marked, one for the test product side and one for the control product side. Before using the sample, the subjects used a skin elasticity test probe (Delfin ElastiMeter) to test the elasticity of the skin and recorded it. After using the test product on the test site, the subjects tested the elasticity of the skin and recorded it.

[0065] 4) Skin wrinkle scoring: 60 subjects aged 30 to 65 with obvious wrinkles were selected, regardless of gender. Two test sites of the subjects were marked, one for the test product side and one for the control product side. Before using the sample, the subjects used a facial image analyzer (Visia) to analyze the distribution and number of wrinkles on the skin and recorded them. After the subjects used the test product continuously for 4 weeks at the test site, the subjects analyzed the distribution and number of wrinkles on the skin and recorded them.

[0066] 5) Skin color ITA° value and melanin index MI value: Select healthy Asian subjects (except pregnant and lactating women) with obvious pigmentation or skin color, regardless of gender. Mark two test sites of the subjects, one for the test product side and one for the control product side. Before using the sample, the subjects use a skin color measuring instrument (Delfin SkinColorCatch) to evaluate and test the color and initial state of melanin, and record them. After the subjects use the test product continuously for 4 weeks at the test site, the skin color ITA° value and skin melanin index MI value are tested and recorded.

[0067] 6) Data statistics: The data were processed by data processing software, and the change rate K before and after itself was: [(mean value after use of the sample - mean value before use of the sample) / mean value before use of the sample] * 100%. The change rate before and after itself K>-0.01% and K<0.01% were invalid. The change rates before and after use of the embodiments and comparative examples were the average results shown in Table 3.

[0068] Table 3: Test results of different effects of examples and comparative examples

[0069]

[0070] According to the different efficacy test results of Examples 1-3 and Comparative Examples 1-3, after using Example 1, the skin is significantly firmer, wrinkles are also improved, and the skin melanin is reduced, which has a whitening effect. Therefore, the application of the large-leaf seaweed extract and the fullerene composition in facial cleansing products can achieve the purpose of reducing skin damage caused by blue light by absorbing blue light, anti-oxidation, resisting / scavenging free radicals, delaying the aging of the skin, and alleviating pigmentation, thereby playing a role in resisting the damage of blue light to the skin.

[0071] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0072] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0073] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A method for extracting a Zona algae extract, characterized in that: The steps include: (1) Impurity removal: Weigh an appropriate amount of large-leaf seaweed, remove impurities, wash it with deionized water until it is clean, then add a certain amount of deionized water, stir it thoroughly, and soak it at room temperature for 1-2 hours; (2) Cell extraction: Take a suitable container and fully mash the soaked Zona algae, filter it with a 100-200 mesh filter cloth, repeat the above steps until the color of the filtrate becomes lighter, collect the filtrate, centrifuge for 3-5 minutes, repeat 3-5 times, remove the upper clear liquid, and collect the Zona algae cell fluid at the lower layer; (3) Cell wall disruption: Add the Zona algae cell solution obtained in step (2) into a high-pressure cell disruptor, introduce carbon dioxide at 0.15-0.3 MPa, adjust the disruption time to 30-40 min, take samples 3-4 times in between, and stop the high-pressure treatment when a large amount of Zona algae cell walls are observed under a microscope to obtain a Zona algae crude solution; (4) extracting essence: placing the macroalgae crude liquid treated in step (3) in an extraction tank for a first supercritical extraction, the extraction time is 100-120 min, the temperature is set to 30° C.-40° C., the extraction pressure is 30-40 MPa, and the flow rate of carbon dioxide is 600 kg / h, to obtain an extract A1 and a residue B1; the residue B1 is subjected to a second supercritical extraction, the extraction time is 60-80 min, the temperature is set to 30° C.-40° C., the extraction pressure is 30-40 MPa, and the flow rate of carbon dioxide is 600 kg / h, to obtain an extract A2; (5) The extracts A1 and A2 are mixed evenly to obtain a macrophylla extract.

2. A blue light protection composition, characterized in that: The invention contains macrophylla extract and fullerene stock solution, and the weight ratio of the two is 0.1-15:0.0001-0.

01.

3. The blue light protection composition according to claim 2, characterized in that: The weight ratio of the macrophylla extract to the fullerene stock solution is 3-15:0.0005-0.

001.

4. The blue light protection composition according to claim 3, characterized in that: The mass percentage of the macrophylla extract to the fullerene stock solution is 4000:

1.

5. A toiletries containing the blue light protection composition according to claim 2 or 3.

6. The toiletries according to claim 5, characterized in that: The content of the blue light protection composition is 0.1001-15.01%.

7. The toiletries according to claim 6, characterized in that: Extracts of macrophylla seaweed make up 3% of the toiletries by mass.

8. The toiletries according to claim 5, characterized in that: The washing product is a facial cleaning product, which contains the following components in percentage by weight: (A) anti-blue light efficacy composition 0.1001-15.01%; (B) surfactant 12-18%; (C) 3-4% thickening stabilizer; (D) pearlescent agent 2-3%; (E) skin conditioning agent 0.5-1%; and the balance (F) Polyol.

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