Composite biological carbon source and preparation method thereof

Abstract

The invention discloses a composite biological carbon source and a preparation method thereof. The composite biological carbon source is prepared from the following raw materials: carbon source liquid, alcohol organic matters, trace elements, small molecular organic acid, saccharides, glycerol, vitamins, a surfactant and a buffering agent, the nutrient solution comprises the following components in parts by weight: 10-30 parts of carbon source liquid, 5-10 parts of alcohol organic matters, 0.5-1 part of trace elements, 1-5 parts of small molecular organic acids, 10-20 parts of saccharides, 5-10 parts of glycerol, 0.5-1 part of vitamins, 0.5-1 part of a surfactant and 0.5-1 part of a buffering agent. The composite biological carbon source is prepared from the carbon source liquid, the alcohol organic matter, the trace elements, the small molecular organic acid, the saccharides, the glycerol, the vitamins, the surfactant and the buffer agent, the components in the composite biological carbon source can be better utilized by microorganisms, the utilization efficiency of the carbon source is improved, and the composite biological carbon source contains various nutritional ingredients, so that the composite biological carbon source has a good application prospect. Various nutritional requirements required by growth of microorganisms can be met, rapid propagation and metabolic activity of the microorganisms are promoted, and the requirements of sewage treatment can be more effectively met.

Description

Technical Field

[0001] The invention relates to the technical field of composite biological carbon source preparation, in particular to a composite biological carbon source and a preparation method thereof. Background Art

[0002] Composite biological carbon source refers to a product formed by combining one or more carbon sources, such as sugars, organic acids, alcohols, etc., with microbial cells or metabolically active substances. It can not only provide the energy necessary for microbial growth, but also be converted into more useful substances through the metabolism of microorganisms. It is mainly used to treat domestic sewage and industrial wastewater, and can effectively remove COD, BOD, etc. The application of composite biological carbon sources in the field of environmental water treatment is mainly reflected in its ability to biodegrade pollutants. When treating organic pollutants in industrial wastewater, specific microbial composite carbon sources can provide necessary nutrients, promote the growth and metabolism of microorganisms, and accelerate the decomposition process of organic matter.

[0003] In wastewater treatment, composite biological carbon sources are widely used in biodegradation and removal of organic pollutants. By providing a variety of carbon sources, they can support the growth and metabolism of a variety of microorganisms, thereby improving the degradation efficiency of organic matter in wastewater. However, the nutritional components of composite biological carbon sources are limited and cannot meet the growth needs of microorganisms. In addition, the utilization rate is low, resulting in poor absorption by microorganisms. Summary of the invention

[0004] The purpose of the present invention is to provide a composite biological carbon source and a preparation method thereof, which has the advantages of not only being able to provide a carbon source, but also being able to regulate water quality and promote the growth of microorganisms, thereby solving the problem that the nutritional components of the composite biological carbon source are limited and cannot meet the growth needs of microorganisms, and the utilization rate is low, resulting in poor microbial absorption effect.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a composite biological carbon source, the preparation raw materials of which include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the proportion is: 10-30 parts of carbon source liquid, 5-10 parts of alcohol organic matter, 0.5-1 part of trace elements, 1-5 parts of small molecule organic acid, 10-20 parts of sugar, 5-10 parts of glycerol, 0.5-1 part of vitamin, 0.5-1 part of surfactant and 0.5-1 part of buffer.

[0006] Preferably, the raw materials for its preparation include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acids, sugars, glycerol, vitamins, surfactants and buffers, and the ratio is: 10 parts of carbon source liquid, 10 parts of alcohol organic matter, 1 part of trace elements, 5 parts of small molecule organic acids, 20 parts of sugars, 10 parts of glycerol, 1 part of vitamins, 1 part of surfactants and 1 part of buffers.

[0007] Preferably, the raw materials for its preparation include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the ratio is: 30 parts of carbon source liquid, 5 parts of alcohol organic matter, 0.5 parts of trace elements, 1 part of small molecule organic acid, 10 parts of sugar, 5 parts of glycerol, 0.5 parts of vitamins, 0.5 parts of surfactant and 0.5 parts of buffer.

[0008] Preferably, the carbon source liquid is extracted from waste crop straw through fermentation, the alcohol organic matter is ethanol, and the trace elements include iron, zinc and copper.

[0009] Preferably, the small molecule organic acid is citric acid, the sugar is sucrose, and the vitamins are vitamin B group.

[0010] Preferably, the preparation method comprises the following steps:

[0011] A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process;

[0012] B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion;

[0013] C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

[0014] Preferably, in step A, aerobic fermentation requires 30-45 days, and anaerobic fermentation requires 15-30 days. The aerobic fermentation temperature should be controlled at 40-50° C., the humidity should be controlled at 50-70%, and the oxygen concentration should be controlled at 10%.

[0015] Preferably, the control standards of the enzymatic hydrolysis treatment in step B are: temperature 30-50°C, pH 5.0-7.0, time 12-24h, and substrate concentration 10-50g / L.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The composite biological carbon source prepared by the present invention is prepared by carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer. The components in the composite biological carbon source can be better utilized by microorganisms, thereby improving the utilization efficiency of the carbon source. The composite biological carbon source also contains a variety of nutrients, which can meet the various nutritional requirements of microbial growth and promote the rapid reproduction and metabolic activities of microorganisms. It can not only provide a carbon source, but also regulate water quality and promote microbial growth. It shows significant advantages in bioavailability, nutritional balance, environmental compatibility, stability, versatility and cost-effectiveness, and can more effectively meet the needs of sewage treatment. DETAILED DESCRIPTION

[0018] A composite biological carbon source and a preparation method thereof. The preparation raw materials include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the proportion is: 10-30 parts of carbon source liquid, 5-10 parts of alcohol organic matter, 0.5-1 part of trace elements, 1-5 parts of small molecule organic acid, 10-20 parts of sugar, 5-10 parts of glycerol, 0.5-1 part of vitamins, 0.5-1 part of surfactant and 0.5-1 part of buffer.

[0019] The preparation method thereof comprises the following steps:

[0020] A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process;

[0021] B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion;

[0022] C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

[0023] Embodiment 2:

[0024] In the first embodiment, the following steps are added:

[0025] The raw materials for its preparation include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the proportion is: 10 parts of carbon source liquid, 10 parts of alcohol organic matter, 1 part of trace elements, 5 parts of small molecule organic acid, 20 parts of sugar, 10 parts of glycerol, 1 part of vitamins, 1 part of surfactant and 1 part of buffer.

[0026] The test results of the composite biocarbon source prepared using the above raw material ratio are as follows:

[0027]

[0028] The preparation method thereof comprises the following steps:

[0029] Embodiment three:

[0030] In the second embodiment, the following steps are added:

[0031] The raw materials for its preparation include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the proportion is: 30 parts of carbon source liquid, 5 parts of alcohol organic matter, 0.5 parts of trace elements, 1 part of small molecule organic acid, 10 parts of sugar, 5 parts of glycerol, 0.5 parts of vitamins, 0.5 parts of surfactant and 0.5 parts of buffer.

[0032] The test results of the composite biocarbon source prepared using the above raw material ratio are as follows:

[0033]

[0034] The preparation method thereof comprises the following steps:

[0035] A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process;

[0036] B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion;

[0037] C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

[0038] Embodiment 4:

[0039] In the third embodiment, the following steps are added:

[0040] The carbon source liquid is extracted from waste crop straw through fermentation, the alcohol organic matter uses ethanol, and the trace elements include iron, zinc and copper.

[0041] The preparation method thereof comprises the following steps:

[0042] A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process;

[0043] B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion;

[0044] C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

[0045] Embodiment five:

[0046] In the fourth embodiment, the following steps are added:

[0047] The small molecule organic acid is citric acid, the sugar is sucrose, and the vitamins are B vitamins.

[0048] The preparation method thereof comprises the following steps:

[0049] A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process;

[0050] B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion;

[0051] C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

[0052] Embodiment six:

[0053] In the fifth embodiment, the following steps are added:

[0054] In step A, aerobic fermentation takes 30-45 days, and anaerobic fermentation takes 15-30 days. The temperature of aerobic fermentation should be controlled at 40-50° C., the humidity should be controlled at 50-70%, and the oxygen concentration should be controlled at 10%.

[0055] The preparation method thereof comprises the following steps:

[0056] A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process;

[0057] B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion;

[0058] C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

[0059] Embodiment seven:

[0060] In the sixth embodiment, the following steps are added:

[0061] The control standards for the enzymatic hydrolysis treatment in step B are: temperature 30-50°C, pH 5.0-7.0, time 12-24h, and substrate concentration 10-50g / L.

[0062] The preparation method thereof comprises the following steps:

[0063] A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process;

[0064] B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion;

[0065] C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

[0066] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0067] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A composite biological carbon source, characterized in that: The preparation raw materials include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the proportion is: 10-30 parts of carbon source liquid, 5-10 parts of alcohol organic matter, 0.5-1 part of trace elements, 1-5 parts of small molecule organic acid, 10-20 parts of sugar, 5-10 parts of glycerol, 0.5-1 part of vitamins, 0.5-1 part of surfactant and 0.5-1 part of buffer.

2. A composite biological carbon source according to claim 1, characterized in that: The raw materials for its preparation include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the proportion is: 10 parts of carbon source liquid, 10 parts of alcohol organic matter, 1 part of trace elements, 5 parts of small molecule organic acid, 20 parts of sugar, 10 parts of glycerol, 1 part of vitamins, 1 part of surfactant and 1 part of buffer.

3. A composite biocarbon source according to claim 1, characterized in that: The raw materials for its preparation include carbon source liquid, alcohol organic matter, trace elements, small molecule organic acid, sugar, glycerol, vitamins, surfactant and buffer, and the proportion is: 30 parts of carbon source liquid, 5 parts of alcohol organic matter, 0.5 parts of trace elements, 1 part of small molecule organic acid, 10 parts of sugar, 5 parts of glycerol, 0.5 parts of vitamins, 0.5 parts of surfactant and 0.5 parts of buffer.

4. A composite biocarbon source according to claim 1, characterized in that: The carbon source liquid is extracted from waste crop straw through fermentation, the alcohol organic matter is ethanol, and the trace elements include iron, zinc and copper.

5. A composite biocarbon source according to claim 1, characterized in that: The small molecule organic acid is citric acid, the sugar is sucrose, and the vitamins are vitamin B group.

6. A method for preparing a composite biological carbon source according to any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: A. First, the crop straw is crushed and squeezed to obtain solid powder and squeezed liquid, and then the solid powder and squeezed liquid are subjected to aerobic fermentation and anaerobic fermentation to obtain carbon source liquid, and trace elements, vitamins and other additives that can promote microbial growth and metabolism are added according to the proportion during the aerobic fermentation and anaerobic fermentation process; B. After the fermentation is completed, the primary carbon source is obtained, and the primary carbon source is subjected to enzymatic hydrolysis. During this process, specific enzymes, dextranase and acid protease are added to convert large molecular proteins and sugars into small molecular alcohols, polypeptides, and amino acids. During the enzymatic hydrolysis process, small molecular organic acids, sugars, glycerol, alcohol organic matter, surfactants, and buffers are added according to the proportion; C. Separating and purifying the carbon source after enzymatic hydrolysis, and obtaining a composite biological carbon source by decolorizing, filtering and concentrating the separated supernatant.

7. A composite biological carbon source and a preparation method thereof according to claim 6, characterized in that: In step A, aerobic fermentation takes 30-45 days, and anaerobic fermentation takes 15-30 days. The aerobic fermentation temperature should be controlled at 40-50° C., the humidity should be controlled at 50-70%, and the oxygen concentration should be controlled at 10%.

8. A composite biological carbon source and a preparation method thereof according to claim 6, characterized in that: The control standards of the enzymatic hydrolysis treatment in step B are: temperature 30-50°C, pH value 5.0-7.0, time 12-24h, and substrate concentration 10-50g / L.

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