Building block type fluorescence spectrum detection system and detection method

Abstract

The invention relates to a building block type fluorescence spectrum detection system and a detection method. The system comprises a building block type bottom plate, a fluorescence excitation module, a filtering focusing module and a fluorescence detection module, the fluorescence excitation module is used for emitting laser and irradiating the laser to a sample to be detected through the dichroscope to excite and generate fluorescence; a driving device of the filtering and focusing module drives a filtering and focusing assembly to move according to the displacement instruction; the fluorescent light is filtered and focused by the filtering and focusing assembly, and the focused fluorescent light passes through a slit of the fluorescence detection module and enters the reflection grating to form diffracted light; the diffracted light is reflected by the reflector to generate reflected light; the imaging assembly collects and processes the reflected light to generate a fluorescence image signal, and sends the fluorescence image signal to the analysis processing device; the analyzing and processing device is used for analyzing and processing the fluorescence image signal to obtain a spectral image of the sample to be detected. Compared with existing fluorescence detection equipment, the building block type fluorescence spectrum detection system is flexible in application, easy to use and low in cost.

Description

Technical Field

[0001] The present invention relates to the technical field of fluorescence spectrum detection, and in particular to a building block type fluorescence spectrum detection system and a detection method. Background Art

[0002] Fluorescence spectroscopy is a technique for qualitative and quantitative analysis of substances based on the property that substances emit fluorescence after absorbing light of a specific wavelength. This technique has the advantages of high sensitivity, high selectivity, and non-destructive analysis, and is widely used in many fields such as biomedicine, environmental monitoring, food safety, and materials science.

[0003] The working principle of fluorescence spectroscopy is to stimulate the fluorescent substance in the sample through a light source to produce excitation light, and then detect and analyze these spectra through a detector to obtain information about the detected substance.

[0004] However, most of the existing fluorescence detection equipment is relatively expensive and difficult to use in practice, such as spectrometers and high-performance liquid chromatographs, which limits the use scenarios of fluorescence spectroscopy detection. Summary of the invention

[0005] The purpose of the present invention is to address the problems existing in the prior art and propose a building block type fluorescence spectrum detection system and detection method. The fluorescence spectrum detection system adopts a modular design, an assembly design adapted to different module designs, and uses 3D building blocks to complete module connection and instrument assembly, which effectively reduces the difficulty of fluorescence spectrum detection, clearly displays the assembly form of each module, and broadens the scope of use of fluorescence spectrum detection.

[0006] To achieve the above-mentioned purpose, in a first aspect, an embodiment of the present invention provides a building block type fluorescence spectrum detection system, the building block type fluorescence spectrum detection system comprising: a building block type base plate, a fluorescence excitation module, a filter focusing module and a fluorescence detection module plugged and fixed on the building block type base plate, a single chip microcomputer and an analysis and processing device;

[0007] The fluorescence excitation module comprises: a laser component, a dichroic mirror component and a sample chamber; the laser emitted by the laser component passes through the dichroic mirror component and irradiates the sample to be tested carried by the sample chamber, and excites the sample to be tested to generate fluorescence;

[0008] The light filtering and focusing module comprises: a driving device and a light filtering and focusing component; the single chip microcomputer sends a displacement instruction to the driving device, and the driving device drives the light filtering and focusing component to move according to the displacement instruction; the fluorescence is reflected to the light filtering and focusing component through the dichroic mirror component; the light filtering and focusing component performs light filtering and focusing on the reflected fluorescence;

[0009] The fluorescence detection module includes: a slit assembly, a reflection grating assembly, a reflector assembly and an imaging assembly; the fluorescence after filtering and focusing passes through the slit assembly and enters the reflection grating assembly to be diffracted to form diffracted light; the diffracted light is reflected by the reflector assembly to generate reflected light; the imaging assembly collects and processes the reflected light to generate a fluorescence image signal, and sends the fluorescence image signal to the analysis and processing device; the analysis and processing device analyzes and processes the fluorescence image signal to obtain a spectral image of the sample to be tested.

[0010] Preferably, the laser assembly comprises: a laser light source fixing seat and a laser light source; the laser light source is plugged and fixed on the building block bottom plate through the laser light source fixing seat;

[0011] The dichroic mirror assembly comprises: a dichroic mirror fixing seat and a dichroic mirror; the dichroic mirror is plugged and fixed on the building block bottom plate through the dichroic mirror fixing seat; the angle between the mirror surface of the dichroic mirror and the laser emitted by the laser light source is 45°;

[0012] The sample chamber comprises: a cuvette fixing seat and a cuvette; the cuvette carries the sample to be tested; the cuvette is plugged and fixed on the building block bottom plate through the cuvette fixing seat;

[0013] The fluorescence excitation module also includes a first light-shielding shell; the dichroic mirror assembly and the sample chamber are placed in the first light-shielding shell; the first light-shielding shell is plugged and fixed on the building block bottom plate; two sides of the first light-shielding shell are respectively provided with a light inlet for laser injection and a light outlet for fluorescence emission;

[0014] The centers of the laser light source, the dichroic mirror and the light inlet are on the same straight line;

[0015] The laser light emitted by the laser light source passes through the light inlet and then through the dichroic mirror to irradiate the sample to be tested carried by the cuvette, and excites the sample to be tested to generate fluorescence;

[0016] The fluorescence is reflected by the dichroic mirror and then passes through the light outlet to reach the filter and focus module.

[0017] Further preferably, the dichroic mirror is a short-pass dichroic mirror.

[0018] Preferably, the driving device comprises: a stepper motor fixing seat, a stepper motor, a driving gear, a rack and a slider fixed to the end of the rack; the stepper motor fixing seat is plugged and fixed to the building block bottom plate; the stepper motor is fixed to the stepper motor fixing seat by bolts; the gear is plugged into the motor shaft of the stepper motor; the gear is meshed with the rack;

[0019] The filter focusing assembly comprises: a bracket fixing seat, a bracket, a filter and a focusing lens; the bracket fixing seat is plugged and fixed on the slider; the bracket is fixed on the bracket fixing seat by a top screw; the filter and the focusing lens are respectively fixed on the bracket by a clamping ring;

[0020] The stepper motor drives the gear to rotate according to the displacement instruction issued by the single chip microcomputer, and the gear drives the rack to move, so that the slider at the end of the rack and the bracket on the slider move to a specified position along the movement direction of the rack;

[0021] The fluorescence reflected by the dichroic mirror assembly is filtered by the filter and then focused by the focusing lens, so that the light beam of the fluorescence is focused onto the slit assembly.

[0022] Further preferably, the filter is a long pass filter.

[0023] Preferably, the slit assembly is plugged and fixed on the building block bottom plate;

[0024] The reflection grating assembly comprises: a reflection grating, a grating seat, a first rotating platform and a first rotating base; the reflection grating is plugged and fixed in the groove of the grating seat; the grating seat is plugged and fixed on the first rotating platform; the first rotating platform is plugged and fixed on the first rotating base; the first rotating base is plugged and fixed on the building block bottom plate;

[0025] The reflector assembly comprises: a reflector, a reflector seat, a second rotating platform and a second rotating base; the reflector is fixed to the reflector seat by bolts; the reflector seat is plugged and fixed to the second rotating platform; the second rotating platform is plugged and fixed to the building block bottom plate;

[0026] The imaging component includes an imaging lens; the imaging component is plugged and fixed on the building block base plate;

[0027] The fluorescence detection module also includes a second light-shielding shell; the second light-shielding shell is plugged and fixed on the building block bottom plate; the second light-shielding shell is provided with a fluorescence inlet and a USB interface; one end of the USB interface is connected to the imaging component, and the other end is connected to the analysis and processing device through a data line;

[0028] The filtered and focused fluorescence passes through the fluorescence inlet and then passes through the slit assembly into the reflection grating;

[0029] The reflection grating diffracts the fluorescence processed by the slit assembly;

[0030] The diffracted fluorescence is reflected to the imaging component through the reflector;

[0031] The imaging component collects the fluorescence reflected by the reflector to generate a fluorescence image signal, and transmits the fluorescence image signal to the analysis and processing device through the data line;

[0032] The analysis and processing device analyzes the acquired fluorescence image signal and generates a spectral image.

[0033] Further preferably, the slit width of the slit assembly includes 0.05mm to 0.2mm;

[0034] The reflection grating has a length of 31 mm, a width of 13 mm and a thickness of 1.3 mm;

[0035] The imaging lens is a CMOS camera; the resolution of the CMOS camera is 1080P and the horizontal pixel is 1920.

[0036] Preferably, before the building block type fluorescence spectrum detection system detects the sample to be tested, the building block type fluorescence spectrum detection system is first calibrated with a standard sample, and the plug-in positions of the fluorescence excitation module, the filter and focusing module, and the fluorescence detection module with the building block base plate, as well as the positions and setting angles of the laser assembly, the filter and focusing assembly, the reflective grating assembly, and the reflector assembly are determined through the calibration.

[0037] In a second aspect, an embodiment of the present invention provides a detection method using the building block type fluorescence spectrum detection system described in the first aspect, the detection method comprising:

[0038] Using standard samples to calibrate the building block fluorescence spectrum detection system;

[0039] The laser component of the fluorescence excitation module emits laser light, so that the laser light passes through the dichroic mirror component of the fluorescence excitation module and irradiates the sample to be tested carried by the sample chamber of the fluorescence excitation module, thereby exciting the sample to be tested to generate fluorescence;

[0040] The dichroic mirror component reflects the fluorescence, so that the reflected fluorescence enters the filter and focus component of the filter and focus module;

[0041] The filtering and focusing component filters and focuses the reflected fluorescence to a slit component of a fluorescence detection module;

[0042] After the fluorescence that has been filtered and focused passes through the slit assembly, it is diffracted in the reflective grating assembly of the fluorescence detection module to form diffracted light;

[0043] The diffracted light is reflected by the reflector assembly of the fluorescence detection module to generate reflected light;

[0044] The imaging component of the fluorescence detection module collects and processes the reflected light to generate a fluorescence image signal, and sends the fluorescence image signal to an analysis and processing device;

[0045] The analysis and processing device analyzes and processes the fluorescence image signal to obtain a spectrum image of the sample to be tested.

[0046] Preferably, the laser assembly comprises: a laser light source fixing seat and a laser light source; the dichroic mirror assembly comprises: a dichroic mirror fixing seat and a dichroic mirror; the sample chamber comprises: a cuvette fixing seat and a cuvette; the driving device comprises: a stepper motor fixing seat, a stepper motor, a driving gear, a rack and a slider arranged on the rack; the filter focusing assembly comprises: a bracket fixing seat, a bracket, a filter and a focusing lens; the reflection grating assembly comprises: a reflection grating, a grating seat, a first rotating platform and a first rotating base; the reflector assembly comprises: a reflector, a reflector seat, a second rotating platform and a second rotating base;

[0047] The detection method comprises:

[0048] The building block type fluorescence spectrum detection system is calibrated, specifically including: placing the standard sample in the cuvette, starting the laser light source to emit laser, so that the laser passes through the dichroic mirror to irradiate the standard sample, and excites the standard sample to generate standard sample fluorescence; the dichroic mirror reflects the standard sample fluorescence, so that the reflected standard sample fluorescence enters the filter and focusing component, passes through the slit component to reach the reflection grating after being filtered by the filter and focused by the focusing lens in sequence, and is diffracted in the reflection grating to form standard diffracted light; the standard diffracted light passes through the The reflector reflects to generate standard reflected light; the imaging component collects and processes the standard reflected light to generate a standard fluorescent image signal and sends it to the analysis and processing device; the analysis and processing device analyzes and processes the standard fluorescent image signal to obtain a test spectrum image; the test spectrum image is compared with the spectrum image of the standard sample in the analysis and processing device to obtain an error value, and the plug-in positions of the fluorescence excitation module, the filter and focus module, and the fluorescence detection module with the building block bottom plate, as well as the positions of the filter and focus component, the grating component, and the reflector component are adjusted according to the error value;

[0049] Wherein, the adjusting of the filter focusing assembly comprises: the single chip computer sends a displacement instruction to the stepping motor, the stepping motor drives the gear to move according to the displacement instruction, the gear drives the rack to move, so that the slider at the end of the rack and the bracket on the slider move to a specified position along the movement direction of the rack; the adjusting of the grating assembly comprises: rotating the first rotating stage according to the error value, so that the first rotating stage drives the reflective grating seat and the reflective grating to rotate to a suitable position; the adjusting of the reflector assembly comprises: rotating the second rotating stage according to the error value, so that the second rotating stage drives the reflector seat and the reflector to rotate to a suitable position;

[0050] After the calibration, the sample to be tested is placed in the cuvette, and the laser light source emits laser light, so that the laser light passes through the dichroic mirror and irradiates the sample to be tested, thereby exciting the sample to be tested to generate fluorescence;

[0051] The dichroic mirror reflects the fluorescence;

[0052] The filter filters the reflected fluorescence and then focuses it through the focusing lens, so that the filtered and focused fluorescence is focused on the slit assembly;

[0053] After the focused fluorescent light passes through the slit assembly, it is diffracted in the reflection grating to form the diffracted light;

[0054] The diffracted light is reflected by the reflector to generate the reflected light;

[0055] The imaging lens in the imaging component collects and processes the reflected light to generate a fluorescent image signal, and transmits the fluorescent image signal to the analysis and processing device through a data line;

[0056] The analysis and processing device analyzes and processes the fluorescence image signal to obtain a spectrum image of the sample to be tested.

[0057] The embodiments of the present invention provide a building block type fluorescence spectrum detection system and detection method, which have the following beneficial effects compared with the prior art.

[0058] (1) In the building block type fluorescence spectrum detection system provided by the embodiment of the present invention, each component in the fluorescence excitation module, the filter focusing module and the fluorescence detection module is fixed on the building block type base plate by plugging. The module replacement design can be carried out according to different usage scenarios. At the same time, the compatibility of the corresponding modules is guaranteed as high as possible, which effectively reduces the difficulty of fluorescence spectrum detection and broadens the application scope of fluorescence spectrum detection.

[0059] (2) The building block type fluorescence spectrum detection system provided in the embodiment of the present invention uses 3D printing technology to manufacture the building block type base plate and the components plugged into the building block type base plate, which can not only ensure the detection accuracy, but also improve the application flexibility of fluorescence spectrum analysis.

[0060] (3) In the building block type fluorescence spectrum detection system provided by the present invention, each module is composed of relatively common and low-cost components, which greatly reduces the cost compared with the existing fluorescence detection equipment. This also makes the building block type fluorescence spectrum detection system of the present invention have a broader market prospect.

[0061] (4) The detection method based on the building block type fluorescence spectrum detection system provided by the embodiment of the present invention is simple and different components can be replaced according to the requirements of different test samples. It is easy to use and has high detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] Figure 1 A structural block diagram of a building block type fluorescence spectrum detection system provided in an embodiment of the present invention.

[0063] Figure 2 A flow chart of a detection method using a building block type fluorescence spectrum detection system provided in an embodiment of the present invention.

[0064] Figure 3 A schematic structural diagram of a building block type fluorescence spectrum detection system according to a preferred solution provided by an embodiment of the present invention.

[0065] Figure 4 A schematic diagram of the three-dimensional structure of a building block type fluorescence spectrum detection system according to a preferred solution provided in an embodiment of the present invention.

[0066] Figure 5 A schematic structural diagram of a fluorescence excitation module of a building block type fluorescence spectrum detection system according to a preferred solution provided in an embodiment of the present invention.

[0067] Figure 6 A schematic structural diagram of a filter and focus adjustment module of a building block type fluorescence spectrum detection system according to a preferred solution provided by an embodiment of the present invention.

[0068] Figure 7 A schematic structural diagram of a fluorescence detection module of a building block type fluorescence spectrum detection system according to a preferred solution provided by an embodiment of the present invention.

[0069] Figure 8 A schematic diagram of the optical path of a building block type fluorescence spectrum detection system according to a preferred embodiment of the present invention

[0070] Fig. 9 This is a spectral image obtained by using the building block type fluorescence spectrum detection system to detect the sample in Example 1 of the present invention. DETAILED DESCRIPTION

[0071] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0072] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments.

[0073] The embodiment of the present invention provides a building block type fluorescence spectrum detection system, such as Figure 1 As shown in the structural block diagram, the building block type fluorescence spectrum detection system 1 includes: a building block type base plate 11, a fluorescence excitation module 12, a filter and focus module 13 and a fluorescence detection module 14 plugged and fixed on the building block type base plate, a single chip computer 15 and an analysis and processing device 16.

[0074] The fluorescence excitation module 12 includes: a laser assembly 121, a dichroic mirror assembly 122 and a sample chamber 123. The laser emitted by the laser assembly 121 passes through the dichroic mirror assembly 122 and irradiates the sample to be tested carried by the sample chamber 123, and excites the sample to be tested to generate fluorescence.

[0075] The filter focusing module 13 includes: a driving device 131 and a filter focusing component 132. The fluorescence generated by the sample to be tested is reflected to the filter focusing component 132 through the dichroic mirror component 122; the filter focusing component 132 performs filter focusing on the reflected fluorescence. Before testing the sample to be tested, the building block type fluorescence spectrum detection system 1 needs to be calibrated. During calibration, the single chip microcomputer 15 sends a displacement instruction to the driving device 131, and the driving device 131 drives the filter focusing component 132 to move to a suitable position according to the displacement instruction.

[0076] The fluorescence detection module 14 includes: a slit assembly 141, a reflection grating assembly 142, a reflector assembly 143 and an imaging assembly 144. The fluorescence after the above filtering and focusing passes through the slit assembly 141 and enters the reflection grating assembly 142 to be diffracted to form diffracted light; the diffracted light is reflected by the reflector assembly 143 to generate reflected light; the imaging assembly 144 collects and processes the reflected light to generate a fluorescence image signal, and sends the fluorescence image signal to the analysis and processing device 16; the analysis and processing device 16 analyzes and processes the fluorescence image signal to obtain a spectral image of the sample to be tested.

[0077] The embodiment of the present invention provides a detection method using the above-mentioned building block type fluorescence spectrum detection system 1, such as Figure 2 As shown, combined Figure 1 The detection method is described, and the detection method specifically includes the following steps:

[0078] Step 110, calibrating the building block type fluorescence spectrum detection system 1 using a standard sample;

[0079] Specifically, the building block fluorescence spectrum detection system 1 is calibrated by standard samples to determine the plug-in positions of the fluorescence excitation module 12, the filter and focusing module 13 and the fluorescence detection module 14 with the building block base plate 11, as well as the positions and setting angles of the laser component 121, the filter and focusing component 132, the reflection grating component 142 and the reflector component 143.

[0080] In step 120 , the laser assembly 121 of the fluorescence excitation module emits laser light, so that the laser light passes through the dichroic mirror assembly 122 of the fluorescence excitation module and irradiates the sample to be tested carried by the sample chamber 123 of the fluorescence excitation module, thereby exciting the sample to be tested to generate fluorescence.

[0081] In step 130 , the dichroic mirror assembly 122 reflects the fluorescence, so that the reflected fluorescence enters the filter and focus assembly 132 of the filter and focus module.

[0082] Step 140 : The filter and focus component 132 filters and focuses the reflected fluorescence to the slit component 141 of the fluorescence detection module.

[0083] Step 150 , after the filtered and focused fluorescence passes through the slit assembly 141 , it is diffracted in the reflective grating assembly 142 of the fluorescence detection module to form diffracted light.

[0084] In step 160 , the diffracted light is reflected by the reflector assembly 143 of the fluorescence detection module to generate reflected light.

[0085] Step 170 , the imaging component 144 of the fluorescence detection module collects and processes the reflected light, generates a fluorescence image signal, and sends the fluorescence image signal to the analysis and processing device 16 .

[0086] In step 180 , the analysis and processing device 16 analyzes and processes the fluorescence image signal to obtain a spectrum image of the sample to be tested.

[0087] Figure 3 A preferred solution of the above-mentioned building block type fluorescence spectrum detection system provided by an embodiment of the present invention, a schematic diagram of the specific structure of the building block type fluorescence spectrum detection system 2 is a top view; Figure 4 The three-dimensional structure diagram of the building block type fluorescence spectrum detection system 2 is shown below. The components of the building block type fluorescence spectrum detection system 2 are described in detail below.

[0088] like Figure 3As shown, the modular fluorescence spectrum detection system 2 includes: a modular base plate 30, a laser assembly 31, a dichroic mirror assembly 32 and a sample chamber 33 of a fluorescence excitation module, a driving device 34 and a filter focusing assembly 35 of a filter focusing module, a slit assembly 36, a reflection grating assembly 37, a reflector assembly 38 and an imaging assembly 39 of a fluorescence detection module. In addition, as Figure 4 As shown, the building block type fluorescence spectrum detection system 2 also includes two light shielding shells, namely the first light shielding shell 41 of the fluorescence excitation module and the second light shielding shell 42 of the fluorescence detection module. Among them, the dichroic mirror assembly 32 and the sample chamber 33 are placed in the first light shielding shell 41; the first light shielding shell 41 is plugged and fixed on the building block bottom plate 30; the two sides of the first light shielding shell 41 are respectively provided with a light inlet 411 for laser injection and a light outlet 412 for fluorescence emission (not shown in the figure). The second light shielding shell 42 includes a shell cover 423 and a shell body 424; the slit assembly 36, the reflection grating assembly 37, the reflector assembly 38 and the imaging assembly 39 are placed in the second light shielding shell 42; the second light shielding shell 42 is plugged and fixed on the building block bottom plate 30; the second light shielding shell 42 is provided with a fluorescence inlet 421 and a USB interface 422; one end of the USB interface 422 is connected to the imaging assembly 39, and the other end is connected to the analysis and processing device (not shown in the figure) through a data line.

[0089] In the present invention, the two light-shielding shells, the first light-shielding shell 41 and the second light-shielding shell 42, are made of opaque material, and the specific material is not particularly limited as long as the light-shielding effect is satisfied; preferably, the specific material may include but is not limited to polylactic acid or modified polylactic acid (Hyper P la), and the color of the light-shielding shell includes but is not limited to black.

[0090] Figure 5 FIG. 2 is a schematic diagram of the overall structure of a fluorescence excitation module including a laser component 31, a dichroic mirror component 32 and a sample chamber 33 in a building block type fluorescence spectrum detection system 2. Figure 5 As shown, the laser assembly 31 specifically includes: a laser light source 50 and a laser light source fixing seat 51 ; the laser light source 50 is plugged and fixed on the building block base plate 30 through the laser light source fixing seat 51 .

[0091] The laser light source 50 includes but is not limited to a laser or a laser pen; the wavelength of the laser emitted by the laser light source 50 can be selected according to different samples to be tested, for example, a laser with a wavelength of 532 nm can be selected.

[0092] The dichroic mirror assembly 32 specifically includes: a dichroic mirror 52 and a dichroic mirror fixing seat 53; the dichroic mirror 52 is plugged and fixed on the building block base plate 30 through the dichroic mirror fixing seat 53; a dichroic mirror fixing seat groove 531 is provided at the lower end of the dichroic mirror fixing seat 53; the angle between the mirror surface of the dichroic mirror 52 and the laser emitted by the laser light source 50 is 45°.

[0093] Among them, the dichroic mirror 32 can transmit the wavelength of the laser, allowing the laser to smoothly enter the sample to be tested to excite and produce fluorescence. The dichroic mirror 32 then reflects the fluorescence with a longer wavelength, thereby guiding the fluorescence to the subsequent filtering and focusing module; the type of dichroic mirror 52 can be selected according to the different samples to be tested. For example, the dichroic mirror 52 can adopt a short-pass dichroic mirror with a reflection band of 590nm to 740nm.

[0094] The centers of the laser light source 50 , the dichroic mirror 52 and the light inlet 411 of the first light shielding housing 41 are on the same straight line.

[0095] The sample chamber 33 specifically includes: a cuvette 54 and a cuvette holder 55; the cuvette 54 is used to carry the sample to be tested; the cuvette 54 is plugged and fixed on the building block bottom plate 30 through the cuvette holder 55. The laser emitted by the laser light source 50 passes through the light inlet 411 and then passes through the dichroic mirror 52 to irradiate the sample to be tested carried by the cuvette 54, and excites the sample to be tested to produce fluorescence.

[0096] Figure 6 It is a schematic diagram of the overall structure of the filter and focus module including the driving device 34 and the filter and focus assembly 35 in the building block type fluorescence spectrum detection system 2.

[0097] like Figure 6 As shown, the driving device 34 specifically includes: a stepper motor 60, a stepper motor fixing seat 61, a gear 62, a rack 63 and a slider 64 fixed to the end of the rack; the stepper motor fixing seat 61 is plugged and fixed on the building block base 30; the stepper motor 60 is fixed to the stepper motor fixing seat 61 by bolts; the gear 62 is plugged on the motor shaft of the stepper motor 60; the gear 62 is meshed with the rack 63; the rack 63 can pass through the groove 531 of the dichroic mirror fixing seat.

[0098] The filter focusing assembly 35 specifically includes: a bracket 65, a bracket fixing seat 66, a filter 67 and a focusing lens 68; the bracket fixing seat 66 is plugged and fixed on the slider 64; the bracket 65 is fixed on the bracket fixing seat 66 by a top screw; the filter 67 and the focusing lens 68 are fixed on the bracket 65 by a retaining ring 69 respectively.

[0099] The parameters of the filter 67 can be selected according to the different samples to be tested, for example, a filter band including but not limited to a filter with a wavelength above 560nm can be selected. The purpose of filtering fluorescence is to remove background light or interference light to ensure that the light signal entering the system is mainly the target fluorescence signal.

[0100] The focusing lens 68 can be selected according to the different samples to be tested, for example, including but not limited to a focusing lens with a focal length of 30 mm. The focusing lens is used to focus the filtered fluorescence to ensure that the light beam is focused on the slit before entering the slit assembly, thereby achieving the best spatial resolution. The use of the focusing lens improves the clarity and signal-to-noise ratio of the fluorescence signal.

[0101] Specifically, the stepper motor 60 drives the gear 62 to rotate according to the displacement instruction issued by the single chip microcomputer (not shown in the figure), and the gear 62 drives the rack 63 to move, so that the slider 64 at the end of the rack 63 and the bracket 65 on the slider 64 move to the specified position along the movement direction of the rack 63.

[0102] After being reflected by the dichroic mirror 52 , the fluorescence is filtered by the filter 67 and then focused by the focusing lens 68 so that the fluorescence beam is focused onto the slit assembly 36 .

[0103] Figure 7 It is a schematic diagram of the overall structure of the fluorescence detection module in the building block type fluorescence spectrum detection system 2, which includes a slit assembly 36, a reflection grating assembly 37, a reflector assembly 38 and an imaging assembly 39.

[0104] like Figure 7 As shown, the slit assembly 36 is plugged and fixed on the building block bottom plate 30, and the slit assembly 36 is provided with a slit 70. The width of the slit 70 is between 0.05 mm and 0.2 mm, and can be any value within this range, such as 0.05 mm, 0.1 mm, 0.2 mm, etc. The fluorescent light focused after filtering and focusing passes through the fluorescent inlet 421 on the second light shielding housing 42 and enters the slit 70. The function of the slit 70 is to limit the width of the fluorescent light beam, thereby improving the spectral resolution and avoiding the interference of redundant light.

[0105] The reflection grating assembly 37 specifically includes: a reflection grating 71, a grating seat 72, a first rotating platform 73 and a first rotating base 74; the reflection grating 71 is plugged and fixed in the groove of the grating seat 72; the grating seat 72 is plugged and fixed on the first rotating platform 73; the first rotating platform 73 is plugged and fixed on the first rotating base 74; the first rotating base 74 is plugged and fixed on the building block base 30.

[0106] The reflection grating 71 diffracts the fluorescence processed by the slit 70. Specifically, the surface of the reflection grating 71 has regular lines or microstructures. When the fluorescence signal is incident on the surface of the reflection grating 71, diffraction occurs due to the wave nature of light. The reflection grating 71 decomposes light of different wavelengths into its component wavelengths, and light of different frequencies is deflected at different angles, thereby achieving spectral separation. Among them, the parameters of the reflection grating 71 can be selected according to different fluorescence wavelengths. For example, the length of the reflection grating 71 can be 31 mm, the width can be 13 mm, and the thickness can be 1.3 mm.

[0107] The reflector assembly 38 specifically includes: a reflector 75, a reflector seat 76, a second rotating platform 77 and a second rotating base 78; the reflector 75 is fixed to the reflector seat 76 by bolts; the reflector seat 76 is plugged and fixed to the second rotating platform 77; the second rotating platform 77 is plugged and fixed to the second rotating base 78; the second rotating base 78 is plugged and fixed to the building block base 30. The diffracted fluorescence generates reflected light through the reflector 75.

[0108] The imaging assembly 39 includes: an imaging lens 79, a lens fixing seat 791, a circuit board 792, a USB interface 422 and a USB interface fixing seat 793; the imaging lens 79 is fixed on the circuit board 792, and the circuit board is fixed on the lens fixing seat 791 by screws; the USB interface 422 is fixed on the USB interface fixing seat 793 by screws; the lens fixing seat 791 and the USB interface fixing seat 793 are respectively plugged and fixed on the building block base 30.

[0109] The imaging lens 79 collects and processes the fluorescence reflected by the reflector 75 to generate a fluorescence image signal, and transmits the fluorescence image signal to the analysis and processing device (not shown in the figure) through a data line; the analysis and processing device (not shown in the figure) analyzes the acquired fluorescence image signal and generates a spectral image. Among them, the imaging lens 79 includes but is not limited to the selection of a CMOS camera, the resolution of the CMOS camera is 1080P, and the horizontal pixel is 1920; the analysis and processing device (not shown in the figure) can be a computer system including hardware and software.

[0110] In the present invention, the building block base plate 30 has protrusions for plugging with other components, and the spacing of the protrusions matches the building blocks on the market. The building block base plate 30 can be printed and manufactured by 3D printing technology with an accuracy of less than or equal to 0.1 mm to ensure assembly error. Preferably, the specific material of the building block base plate 30 can include but is not limited to polylactic acid or modified polylactic acid (Hyper P la).

[0111] In addition, the building block bottom plate 30 further includes building block bottom plate fixing holes 301 for fixing the building block bottom plate 30 on the table top. There may be multiple building block bottom plate fixing holes.

[0112] The optical path schematic diagram of the building block type fluorescence spectrum detection system 2 provided in the embodiment of the present invention is as follows: Figure 8 As shown, the specific path of light is as follows: the laser light source emits a laser beam, which passes through a dichroic mirror and irradiates the sample to be tested in the cuvette, exciting the sample to be tested to generate a fluorescent beam. The fluorescent beam is reflected by the dichroic mirror and enters the filter and focusing assembly. After filtering and focusing, it is focused and passes through the slit to reach the reflection grating. The diffraction light is formed by the diffraction of the reflection grating. The diffraction light is then reflected by the reflector to form reflected light. The imaging lens collects and processes the reflected light.

[0113] The embodiment of the present invention provides a detection method using the above-mentioned building block type fluorescence spectrum detection system 2, combined with Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7 The specific detection method steps are described as follows:

[0114] Step 210, calibrating the building block type fluorescence spectrum detection system 2;

[0115] The calibration process specifically includes: placing the standard sample in the cuvette 54, starting the laser light source 50 to emit laser light, so that the laser light passes through the dichroic mirror 52 to irradiate the standard sample, and excites the standard sample to generate standard sample fluorescence; the dichroic mirror 52 reflects the standard sample fluorescence, so that the reflected standard sample fluorescence enters the filter and focusing assembly 35, and is filtered by the filter 67 and focused by the focusing lens 68 in turn, and then focuses through the slit assembly 36 to reach the reflection grating 71, and is diffracted in the reflection grating 71 to form standard diffracted light; the standard diffracted light is reflected by the reflector 75 to generate standard reflected light; The imaging component 39 collects and processes the standard reflected light, generates a standard fluorescence image signal and sends it to the analysis and processing device (not shown in the figure); the analysis and processing device (not shown in the figure) analyzes and processes the standard fluorescence image signal to obtain a test spectrum image; the test spectrum image is compared with the spectrum image of the standard sample in the analysis and processing device (not shown in the figure) to obtain an error value, and the plug-in position of each component in the building block type fluorescence spectrum detection system 2 and the building block type bottom plate, as well as the position and angle of the filter focusing component 35, the reflection grating component 37 and the reflector component 38 are adjusted according to the error value;

[0116] Among them, the specific process of adjusting the filter focusing component 35 includes: using a single-chip microcomputer (not shown in the figure) to send a displacement instruction to the stepper motor 60, the stepper motor 60 drives the gear 62 to move according to the displacement instruction, the gear 62 drives the rack 63 to move, so that the slider 64 at the end of the rack 63 and the bracket 65 on the slider 64 move to a suitable position along the movement direction of the rack 63; adjusting the reflection grating component 37 includes: manually rotating the first rotating table 73 according to the error value, so that the first rotating table 73 drives the reflection grating seat 72 and the reflection grating 71 to rotate to a suitable position; adjusting the reflector component 38 includes: manually rotating the second rotating table 77 according to the error value, so that the second rotating table 77 drives the reflector seat 76 and the reflector 75 to rotate to a suitable position.

[0117] Step 220 , after calibrating the building block type fluorescence spectrum detection system 2 , the sample to be tested is placed in the cuvette 54 , and the laser light source 50 emits laser light, so that the laser light passes through the dichroic mirror 52 and irradiates the sample to be tested, thereby exciting the sample to be tested to generate fluorescence.

[0118] In step 230 , the dichroic mirror 52 reflects the fluorescence generated by the sample to be tested.

[0119] In step 240 , the filter 67 filters the reflected fluorescence and then focuses it through the focusing lens 68 , so that the filtered and focused fluorescence is focused onto the slit 70 of the slit assembly 36 .

[0120] In step 250 , the focused fluorescent light passes through the slit 70 and is diffracted in the reflection grating 71 to form diffracted light.

[0121] In step 260 , the diffracted light is reflected by the reflector 75 to generate reflected light.

[0122] Step 270, the imaging lens 79 in the imaging component 39 collects and processes the reflected light to generate a fluorescent image signal, and transmits the fluorescent image signal to an analysis and processing device (not shown in the figure) through a data line; wherein the imaging lens 79 uses a CMOS camera.

[0123] Step 280: an analysis and processing device (not shown) analyzes and processes the fluorescent image signal to obtain a spectral image of the sample to be tested.

[0124] Example 1

[0125] The embodiment of the present invention provides a method for using the above-mentioned building block fluorescence spectrum detection system to detect fluorescent brightening agents (Fluorescent Brightener, CAS No. 7128-64-5) at different concentrations; the sample concentrations of the fluorescent brightening agents are 1×10 -2 mol / L、1×10 -3mol / L、1×10 -4 mol / L、1×10 -5 mol / L、1×10 -6 mol / L、3×10 -6 mol / L、5×10 -6 mol / L, wherein the solvent is dimethyl sulfoxide (DSMO).

[0126] The specific detection process is as follows:

[0127] (1) The samples to be tested are placed in different cuvettes. The laser light source is a 405nm laser pen, which emits a laser with a wavelength of 405nm. The laser is irradiated onto the samples to be tested through a short-pass dichroic mirror, exciting the samples to produce fluorescence.

[0128] (2) The short-pass dichroic mirror reflects the fluorescence generated by the sample to be tested.

[0129] (3) The filter filters the reflected fluorescence and then focuses it through a focusing lens, so that the filtered and focused fluorescence is focused on the slit of the slit assembly. The focal length of the focusing lens is 30 mm, and the inner width of the slit is 0.1 mm.

[0130] (4) After the focused fluorescence passes through the slit, it is diffracted in the reflection grating to form diffracted light. The reflection grating is 31 mm long, 13 mm wide and 1.3 mm thick.

[0131] (5) The diffracted light is reflected by a reflector to generate reflected light. The diameter of the reflector is 25.4 mm.

[0132] (6) The CMOS camera collects and processes the reflected light to generate a fluorescent image signal, and transmits the fluorescent image signal to the computer system through a data line. The resolution of the CMOS camera is 1080P and the horizontal pixel is 1920.

[0133] (7) The computer system analyzes and processes the fluorescent image signal to obtain spectral images of samples of fluorescent whitening reagent with different concentrations.

[0134] The spectral images of the fluorescent whitening reagent at different concentrations generated by the detection in this embodiment are as follows: Fig. 9As shown, the horizontal axis is wavelength (Wave length, unit nm), and the vertical axis is intensity (Intensity, unit au). It can be seen that a fluorescence peak is generated in the wavelength range of 430nm to 490nm, which is consistent with the actual spectral information of the fluorescent whitening agent, and the greater the sample concentration of the fluorescent whitening agent, the greater the intensity of the characteristic peak. This proves that the building block type fluorescence spectrum detection system provided in the embodiment of the present invention can adapt to different usage scenario requirements and realize fluorescence spectrum analysis of more wavelengths.

[0135] The professionals should further realize that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented by electronic hardware, computer software, or a combination of the two. In order to clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described in the above description according to the function. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professionals and technicians can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of the present invention.

[0136] The steps of the method or algorithm described in conjunction with the embodiments disclosed herein may be implemented using hardware, a software module executed by a processor, or a combination of the two. The software module may be placed in a random access memory (RAM), a memory, a read-only memory (ROM), an electrically programmable ROM, an electrically erasable programmable ROM, a register, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art.

[0137] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A building block type fluorescence spectrum detection system, characterized in that: The building block type fluorescence spectrum detection system comprises: a building block type base plate, a fluorescence excitation module, a filter focusing module and a fluorescence detection module plugged and fixed on the building block type base plate, a single chip microcomputer and an analysis and processing device; The fluorescence excitation module comprises: a laser component, a dichroic mirror component and a sample chamber; the laser emitted by the laser component passes through the dichroic mirror component and irradiates the sample to be tested carried by the sample chamber, and excites the sample to be tested to generate fluorescence; The light filtering and focusing module comprises: a driving device and a light filtering and focusing component; the single chip microcomputer sends a displacement instruction to the driving device, and the driving device drives the light filtering and focusing component to move according to the displacement instruction; the fluorescence is reflected to the light filtering and focusing component through the dichroic mirror component; the light filtering and focusing component performs light filtering and focusing on the reflected fluorescence; The fluorescence detection module includes: a slit assembly, a reflection grating assembly, a reflector assembly and an imaging assembly; the fluorescence after filtering and focusing passes through the slit assembly and enters the reflection grating assembly to be diffracted to form diffracted light; the diffracted light is reflected by the reflector assembly to generate reflected light; the imaging assembly collects and processes the reflected light to generate a fluorescence image signal, and sends the fluorescence image signal to the analysis and processing device; the analysis and processing device analyzes and processes the fluorescence image signal to obtain a spectral image of the sample to be tested.

2. The building block type fluorescence spectrum detection system according to claim 1, characterized in that: The laser assembly comprises: a laser light source fixing seat and a laser light source; the laser light source is plugged and fixed on the building block bottom plate through the laser light source fixing seat; The dichroic mirror assembly comprises: a dichroic mirror fixing seat and a dichroic mirror; the dichroic mirror is plugged and fixed on the building block bottom plate through the dichroic mirror fixing seat; the angle between the mirror surface of the dichroic mirror and the laser emitted by the laser light source is 45°; The sample chamber comprises: a cuvette fixing seat and a cuvette; the cuvette carries the sample to be tested; the cuvette is plugged and fixed on the building block bottom plate through the cuvette fixing seat; The fluorescence excitation module also includes a first light-shielding shell; the dichroic mirror assembly and the sample chamber are placed in the first light-shielding shell; the first light-shielding shell is plugged and fixed on the building block bottom plate; two sides of the first light-shielding shell are respectively provided with a light inlet for laser injection and a light outlet for fluorescence emission; The centers of the laser light source, the dichroic mirror and the light inlet are on the same straight line; The laser emitted by the laser light source passes through the light inlet and then through the dichroic mirror to irradiate the sample to be tested carried by the cuvette, and excites the sample to be tested to generate fluorescence; The fluorescence is reflected by the dichroic mirror and then passes through the light outlet to reach the filtering and focusing module.

3. The building block type fluorescence spectrum detection system according to claim 2, characterized in that: The dichroic mirror is a short-pass dichroic mirror.

4. The building block type fluorescence spectrum detection system according to claim 1, characterized in that: The driving device comprises: a stepper motor fixing seat, a stepper motor, a driving gear, a rack and a slider fixed to the end of the rack; the stepper motor fixing seat is plugged and fixed to the building block type bottom plate; the stepper motor is fixed to the stepper motor fixing seat by bolts; the gear is plugged on the motor shaft of the stepper motor; the gear is meshed with the rack; The filter focusing assembly comprises: a bracket fixing seat, a bracket, a filter and a focusing lens; the bracket fixing seat is plugged and fixed on the slider; the bracket is fixed on the bracket fixing seat by a top screw; the filter and the focusing lens are respectively fixed on the bracket by a clamping ring; The stepper motor drives the gear to rotate according to the displacement instruction issued by the single chip microcomputer, and the gear drives the rack to move, so that the slider at the end of the rack and the bracket on the slider move to a specified position along the movement direction of the rack; The fluorescence reflected by the dichroic mirror assembly is filtered by the filter and then focused by the focusing lens, so that the light beam of the fluorescence is focused onto the slit assembly.

5. The building block type fluorescence spectrum detection system according to claim 4, characterized in that: The optical filter is a long pass filter.

6. The building block type fluorescence spectrum detection system according to claim 1, characterized in that: The slit assembly is plugged and fixed on the building block bottom plate; The reflection grating assembly comprises: a reflection grating, a grating seat, a first rotating platform and a first rotating base; the reflection grating is plugged and fixed in the groove of the grating seat; the grating seat is plugged and fixed on the first rotating platform; the first rotating platform is plugged and fixed on the first rotating base; the first rotating base is plugged and fixed on the building block bottom plate; The reflector assembly comprises: a reflector, a reflector seat, a second rotating platform and a second rotating base; the reflector is fixed to the reflector seat by bolts; the reflector seat is plugged and fixed to the second rotating platform; the second rotating platform is plugged and fixed to the building block bottom plate; The imaging component includes an imaging lens; the imaging component is plugged and fixed on the building block base plate; The fluorescence detection module also includes a second light-shielding shell; the second light-shielding shell is plugged and fixed on the building block bottom plate; the second light-shielding shell is provided with a fluorescence inlet and a USB interface; one end of the USB interface is connected to the imaging component, and the other end is connected to the analysis and processing device through a data line; The filtered and focused fluorescence passes through the fluorescence inlet and then passes through the slit assembly into the reflection grating; The reflection grating diffracts the fluorescence processed by the slit assembly; The diffracted fluorescence is reflected to the imaging component through the reflector; The imaging component collects the fluorescence reflected by the reflector to generate a fluorescence image signal, and transmits the fluorescence image signal to the analysis and processing device through the data line; The analysis and processing device analyzes the acquired fluorescence image signal and generates a spectral image.

7. The building block type fluorescence spectrum detection system according to claim 6, characterized in that: The slit width of the slit assembly includes 0.05mm to 0.2mm; The reflection grating has a length of 31 mm, a width of 13 mm and a thickness of 1.3 mm; The imaging lens is a CMOS camera; the resolution of the CMOS camera is 1080P and the horizontal pixel is 1920.

8. The building block type fluorescence spectrum detection system according to claim 1, characterized in that: Before the building block type fluorescence spectrum detection system detects the sample to be tested, the building block type fluorescence spectrum detection system is first calibrated with a standard sample, and the plug-in positions of the fluorescence excitation module, the filter and focusing module, and the fluorescence detection module with the building block base plate, as well as the positions and setting angles of the laser assembly, the filter and focusing assembly, the reflective grating assembly, and the reflector assembly are determined through the calibration.

9. A detection method using the building block type fluorescence spectrum detection system according to any one of claims 1 to 8, characterized in that: The detection method comprises: Using standard samples to calibrate the building block fluorescence spectrum detection system; The laser component of the fluorescence excitation module emits laser light, so that the laser light passes through the dichroic mirror component of the fluorescence excitation module and irradiates the sample to be tested carried by the sample chamber of the fluorescence excitation module, thereby exciting the sample to be tested to generate fluorescence; The dichroic mirror component reflects the fluorescence, so that the reflected fluorescence enters the filter and focus component of the filter and focus module; The filtering and focusing component filters and focuses the reflected fluorescence to a slit component of a fluorescence detection module; After the fluorescence that has been filtered and focused passes through the slit assembly, it is diffracted in the reflective grating assembly of the fluorescence detection module to form diffracted light; The diffracted light is reflected by the reflector assembly of the fluorescence detection module to generate reflected light; The imaging component of the fluorescence detection module collects and processes the reflected light to generate a fluorescence image signal, and sends the fluorescence image signal to an analysis and processing device; The analysis and processing device analyzes and processes the fluorescent image signal to obtain a spectral image of the sample to be tested.

10. The detection method according to claim 9, characterized in that: The laser assembly includes: a laser light source fixing seat and a laser light source; the dichroic mirror assembly includes: a dichroic mirror fixing seat and a dichroic mirror; the sample chamber includes: a cuvette fixing seat and a cuvette; the driving device includes: a stepping motor fixing seat, a stepping motor, a driving gear, a rack and a slider arranged on the rack; the filter focusing assembly includes: a bracket fixing seat, a bracket, a filter and a focusing lens; the reflection grating assembly includes: a reflection grating, a grating seat, a first rotating platform and a first rotating base; the reflector assembly includes: a reflector, a reflector seat, a second rotating platform and a second rotating base; The detection method comprises: The building block type fluorescence spectrum detection system is calibrated, specifically including: placing the standard sample in the cuvette, starting the laser light source to emit laser, so that the laser passes through the dichroic mirror to irradiate the standard sample, and excites the standard sample to generate standard sample fluorescence; the dichroic mirror reflects the standard sample fluorescence, so that the reflected standard sample fluorescence enters the filter and focusing component, passes through the slit component to reach the reflection grating after being filtered by the filter and focused by the focusing lens in sequence, and is diffracted in the reflection grating to form standard diffracted light; the standard diffracted light passes through the The reflector reflects to generate standard reflected light; the imaging component collects and processes the standard reflected light to generate a standard fluorescent image signal and sends it to the analysis and processing device; the analysis and processing device analyzes and processes the standard fluorescent image signal to obtain a test spectrum image; the test spectrum image is compared with the spectrum image of the standard sample in the analysis and processing device to obtain an error value, and the plug-in positions of the fluorescence excitation module, the filter and focus module, and the fluorescence detection module with the building block bottom plate, as well as the positions of the filter and focus component, the grating component, and the reflector component are adjusted according to the error value; Wherein, the adjusting of the filter focusing assembly comprises: the single chip computer sends a displacement instruction to the stepping motor, the stepping motor drives the gear to move according to the displacement instruction, the gear drives the rack to move, so that the slider at the end of the rack and the bracket on the slider move to a specified position along the movement direction of the rack; the adjusting of the grating assembly comprises: rotating the first rotating stage according to the error value, so that the first rotating stage drives the reflective grating seat and the reflective grating to rotate to a suitable position; the adjusting of the reflector assembly comprises: rotating the second rotating stage according to the error value, so that the second rotating stage drives the reflector seat and the reflector to rotate to a suitable position; After the calibration, the sample to be tested is placed in the cuvette, and the laser light source emits laser light, so that the laser light passes through the dichroic mirror and irradiates the sample to be tested, thereby exciting the sample to be tested to generate fluorescence; The dichroic mirror reflects the fluorescence; The filter filters the reflected fluorescence and then focuses it through the focusing lens, so that the filtered and focused fluorescence is focused on the slit assembly; After the focused fluorescent light passes through the slit assembly, it is diffracted in the reflection grating to form the diffracted light; The diffracted light is reflected by the reflector to generate the reflected light; The imaging lens in the imaging component collects and processes the reflected light to generate a fluorescent image signal, and transmits the fluorescent image signal to the analysis and processing device through a data line; The analysis and processing device analyzes and processes the fluorescent image signal to obtain a spectral image of the sample to be tested.

Images