Field rapid evaluation method of sesbania lignin

Abstract

The invention relates to the field of crop quality identification, and particularly provides a field rapid evaluation method of sesbania lignin. According to the method, a hydrochloric acid-phloroglucinol solution is used for dyeing the cross section of a sesbania stem growing in the field, and lignin of different sesbania materials is rapidly evaluated in the field. The method can rapidly and efficiently evaluate the lignin content of sesbania, is simple and easy to operate, can be carried out in the field and does not depend on laboratory instruments and equipment, so that the evaluation efficiency of the germplasm resources of sesbania is improved, the screening of new varieties is accelerated, and the method is suitable for industrial popularization and application.

Description

Technical Field

[0001] The invention belongs to the field of crop quality identification and relates to a field rapid evaluation method for sesbania lignin. Background Art

[0002] Sesbania cannabina is a plant of the Fabaceae family, Fabaceae subfamily, and genus Sesbania. It has excellent characteristics such as waterlogging resistance, salt-alkali resistance, and barrenness resistance. The sustainable development of animal husbandry is inseparable from the supply of high-quality forage resources. The stems and leaves of Sesbania cannabina are rich in nutrients and have a high crude protein content. They can be made into silage or harvested and made into hay for storage. They have the potential to become high-quality protein forage. In addition, Sesbania cannabina has a large biomass and can be mowed many times, making it an ideal material for bioenergy crops.

[0003] Lignin is a key factor affecting forage quality and bioenergy utilization. It reduces the digestibility of forage, affects the palatability of forage and livestock intake rate, and hinders the degradation and utilization of structural polysaccharides by cellulase. Lignin has always been considered a substance that cannot be digested and utilized by livestock, which seriously affects the utilization efficiency of nutrients in forage. The content of lignin is negatively correlated with the rumen degradation rate of forage and livestock intake rate.

[0004] The content of lignin is a key indicator for evaluating forage quality. At present, the detection of sesbania lignin relies on some traditional methods such as redox reaction titration, concentrated acid hydrolysis separation determination, photometry, etc. However, these methods are cumbersome, time-consuming, costly and cannot achieve rapid detection in the field. At present, there is a lack of a method for rapid evaluation of sesbania lignin in the field. Summary of the invention

[0005] The invention aims to provide a field rapid evaluation method of sesbania lignin which is simple to operate, short in time consumption, high in efficiency and independent of laboratory operation.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A method for rapid field evaluation of Sesbania lignin is carried out in the following steps:

[0008] S1 Preparation of hydrochloric acid-phloroglucinol solution;

[0009] S2 Cross section of a stalk of Sesbania sesbania growing normally in the field;

[0010] S3 staining of the cross-section of the stalk of Sesbania sesbania;

[0011] Observation and comparison of S4 staining results;

[0012] The position of cross-cutting the sesbania stem in step S2 is the middle part of the sesbania stem, i.e., 1 / 2, and the cutting direction is cross-cutting and perpendicular to the growth direction of the stem.

[0013] Furthermore, the concentration of the hydrochloric acid-phloroglucinol solution in step S1 is 1% v / v.

[0014] Furthermore, in step S3, the amount of hydrochloric acid-phloroglucinol solution added is 3 drops to ensure that the cross section can be completely wetted, and the staining time is 45 seconds.

[0015] Furthermore, in the staining result in step S4, the darker the coloring degree of the cross section and the larger the coloring area, the higher the lignin content.

[0016] In a specific embodiment, the 1% v / v hydrochloric acid-phloroglucinol solution in step S1 is prepared by taking 5 g of phloroglucinol, adding 400 ml of 12% v / v hydrochloric acid to fully dissolve it, and then setting the volume to 500 ml in a volumetric flask.

[0017] Beneficial effects of the present invention:

[0018] The present invention provides for the first time a method for rapid field evaluation of sesbania lignin. After the sesbania stems grown in the field are cut transversely, a hydrochloric acid-phloroglucinol solution is used to stain the sesbania stem lignin. The sesbania lignin content can be evaluated by the degree of stem coloring, and sesbania germplasm with low lignin content can be preliminarily screened.

[0019] The method of the present invention is simple to operate, low in cost, short in time and does not rely on a laboratory platform, and can realize the rapid field evaluation of sesbania lignin, and the result is intuitive and reliable. The present invention can efficiently, quickly and intuitively evaluate the lignin in sesbania stems, thereby improving the quality evaluation efficiency of germplasm resources and the screening efficiency of new low-lignin varieties, and is suitable for promotion and application in the sesbania industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a rapid evaluation of the lignin of different Sesbania materials in the field; A is the cross section of the stem of material YS06055, B is the cross section of the stem of material YS05093, C is the cross section of the stem of YS08001, and D is the cross section of the stem of YS10001.

[0021] Figure 2 This is a schematic diagram of different cross-sectional areas of Sesbania tatarica.

[0022] Figure 3 It is a comparison of cross sections of different Sesbania materials and different stem parts; A is the cross section of the stem of material YS06055, B is the cross section of the stem of material YS05093, C is the cross section of the stem of YS08001, and D is the cross section of the stem of YS10001.

[0023] Figure 4 This is a comparison of different staining times; A is staining for 15 s, B is staining for 30 s, C is staining for 45 s, and D is staining for 60 s. DETAILED DESCRIPTION

[0024] The specific implementation modes of the present invention are described below so that those skilled in the art can understand the present invention. However, it should be clear that the present invention is not limited to the scope of the specific implementation modes. For those of ordinary skill in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the attached claims, these changes are obvious, and all inventions and creations utilizing the concept of the present invention are protected.

[0025] The reagents used in the following examples are: 12% hydrochloric acid, phloroglucinol solid powder

[0026] Example 1: A rapid field evaluation method for Sesbania tinctoria lignin

[0027] 1. Preparation of hydrochloric acid-phloroglucinol solution

[0028] The hydrochloric acid-phloroglucinol solution is prepared as needed: take 5 g of phloroglucinol, add 400 ml of 12% hydrochloric acid to fully dissolve it, and then dilute to 500 ml in a volumetric flask.

[0029] 2. Cross-section of the stalks of Sesbania sesbania growing in the field

[0030] Cut the stalk horizontally at the middle part, i.e. 1 / 2, and remove the upper half.

[0031] 3. Staining of cross-section of sesbania stems

[0032] Use a rubber-tipped dropper to absorb the prepared hydrochloric acid-phloroglucinol solution, use a clean paper towel to gently wipe off the juice secreted from the cut of the sesbania stalk, and immediately add 3 drops of hydrochloric acid-phloroglucinol solution to the cross-section to ensure that the cross-section is completely soaked. After staining for 45 seconds, wipe off the solution with a clean paper towel.

[0033] 4. Observation of dyeing results and comparison between different materials

[0034] After dyeing, the cross section of the stalk will show different coloring effects due to the difference in lignin content. The higher the lignin content, the darker the coloring and the larger the coloring area. Figure 1 As shown, there are large differences in the degree of coloration between different materials, among which YS10001 has the weakest coloration and the lowest lignin content, and YS05093 has the darkest coloration and the highest lignin content.

[0035] Example 2: Effect of different dyeing sites on the evaluation of sesbania lignin content

[0036] 1. Preparation of hydrochloric acid-phloroglucinol solution

[0037] The hydrochloric acid-phloroglucinol solution is prepared as needed: take 5 g of phloroglucinol, add 400 ml of 12% hydrochloric acid to fully dissolve it, and then dilute to 500 ml in a volumetric flask.

[0038] 2. Selection of different sesbania materials

[0039] Different sesbania materials that were in the initial flowering stage and were grown for one month included YS06055, YS05093, YS08001, and YS10001.

[0040]

[0041] 3. Cross-section of different parts of the stalk of Sesbania sesbania

[0042] Make cross-sections at 5 / 6, 1 / 2, and 1 / 6 of the stalk of Sesbania in order. Figure 2 shown.

[0043] 4. Staining of cross-sections of Sesbania sesbania stems

[0044] Use a rubber-tipped dropper to absorb the prepared hydrochloric acid-phloroglucinol solution, use a clean paper towel to gently wipe off the juice secreted from the cut of the sesbania stalk, and immediately add 3 drops of hydrochloric acid-phloroglucinol solution to the cross-section to ensure that the cross-section is completely soaked. After staining for 45 seconds, wipe off the solution with a clean paper towel.

[0045] 5. Comparison of staining results at different cross-section positions of different sesbania materials

[0046] The degree of staining at different cross-section positions of different sesbania materials was compared to determine the location with the greatest difference between the materials. Figure 3 As shown, when the cross-section position is 1 / 6 and 5 / 6 of the stem, there is no significant difference in the degree of coloring between different materials, but at the 1 / 2 position, there is a clear difference in the degree of coloring between different materials.

[0047] Example 3: Effect of different dyeing times on the evaluation of sesbania lignin

[0048] 1. Preparation of hydrochloric acid-phloroglucinol solution

[0049] The hydrochloric acid-phloroglucinol solution is prepared as needed: take 5 g of phloroglucinol, add 400 ml of 12% hydrochloric acid to fully dissolve it, and then dilute to 500 ml in a volumetric flask.

[0050] 2. Cross-section of the stalks of Sesbania sesbania growing in the field

[0051] Cut the stalk in the middle, i.e. 1 / 2 of the way through, and remove the upper half.

[0052] 3. Staining of cross-section of sesbania stems

[0053] Use a rubber-tipped dropper to absorb the prepared hydrochloric acid-phloroglucinol solution, use a clean paper towel to gently wipe off the juice secreted from the cut of the stalk of Sesbania sesbania, and immediately add 3 drops of hydrochloric acid-phloroglucinol solution to the cross-section to ensure that the cross-section is completely soaked. After staining for 15s, 30s, 45s, and 60s, take photos to record the degree of coloration of the cross-section.

[0054] 4. Comparison of staining results of Sesbania sesbania stem cross sections at different staining times

[0055] The cross-section staining results at different staining times were compared. Figure 4 As shown in the figure, when the dyeing time is 45s, the coloring degree tends to be stable and there is no difference with dyeing for 60s.

[0056] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for rapid field evaluation of Sesbania juncea lignin, characterized in that: The following steps are involved: S1 Preparation of hydrochloric acid-phloroglucinol solution; S2 Cross section of a stalk of Sesbania sesbania growing normally in the field; S3 staining of the cross-section of the sesbania stem; Observation and comparison of S4 staining results; The position of cross-cutting the sesbania stem in step S2 is the middle part of the sesbania stem, i.e., 1 / 2, and the cutting direction is cross-cutting and perpendicular to the growth direction of the stem.

2. The method according to claim 1, characterized in that: The concentration of the hydrochloric acid-phloroglucinol solution in step S1 is 1% v / v.

3. The method according to claim 1, characterized in that: In step S3, the amount of hydrochloric acid-phloroglucinol solution added is 3 drops to ensure that the cross section can be completely wetted, and the staining time is 45 seconds.

4. The method according to claim 1, characterized in that: In the dyeing result in step S4, the darker the coloring degree of the cross section and the larger the coloring area, the higher the lignin content.

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