Preparation method of antioxidant plant extract and application of antioxidant plant extract in cosmetics

Abstract

The invention provides an antioxidant plant extract. The antioxidant plant extract comprises the following components in parts by weight: carrots, scutellaria baicalensis, grape seeds, pomegranate seeds and green tea. Green tea, radix scutellariae, carrots, grape seeds and pomegranate seeds are prepared respectively, and corresponding extraction processes are adopted, so that the stability of all the components is improved, and all the effective components have a synergistic effect; the antioxidant is applied to preparation of cosmetics and shows good oxidation resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of plant extracts, and specifically relates to a preparation method of an antioxidant plant extract and application thereof in cosmetics. Background Art

[0002] Plant extracts refer to natural substances extracted from plants, which can be in liquid, solid or semi-solid form. Extracts may contain a variety of components, such as alkaloids, flavonoids, polyphenols, terpenoids, etc., which have different biological activities and pharmacological effects.

[0003] Plant extracts are widely used in many fields such as medicine, food additives, health supplements, cosmetics and personal care products. Depending on the extraction method, products of different purity and composition can be obtained. Common extraction methods include solvent extraction (using water, ethanol or other organic solvents), distillation, supercritical fluid extraction, etc. Antioxidant plant extracts are those plant extracts that contain natural antioxidant ingredients that can help fight oxidative damage caused by free radicals. Free radicals are a class of very active molecules that can cause oxidative stress when they are too abundant in the body, which can lead to cell damage, inflammation and even certain diseases such as cardiovascular disease, cancer and accelerated aging process.

[0004] At present, in the preparation of antioxidant plant extracts, there are many types of raw materials with varying effects, and the various plant components are easily affected by each other, resulting in unstable antioxidant properties or even reduced effects; therefore, it is of practical significance to develop a method for preparing antioxidant plant extracts and apply them in cosmetics. Summary of the invention

[0005] The purpose of the present invention is to provide an antioxidant plant extract and a preparation method thereof, and to apply the same in cosmetics. By using plants such as carrots, scutellaria, grape seeds, pomegranate seeds, green tea and the like as raw materials, improving the configuration of extraction components and the extraction process, improving the stability of each component, and synergizing the effective components, the extract has good antioxidant properties.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: An antioxidant plant extract comprises the following components by weight: 4-8 parts of carrots, 7-12 parts of scutellaria baicalensis, 8-12 parts of grape seeds, 8-14 parts of pomegranate seeds and 3-7 parts of green tea.

[0007] Furthermore, 6 parts of carrots, 10 parts of scutellaria baicalensis, 10 parts of grape seeds, 12 parts of pomegranate seeds, and 5 parts of green tea.

[0008] The method for preparing the above-mentioned antioxidant plant extract comprises the following steps: S1: Wash, dry and crush 3-7 parts of green tea by weight, add deionized water at a solid-liquid ratio of 1:8-12, reflux and extract at 90-100°C for 2-4 times, each time for 1-2 hours, combine the extracted extracts, filter the residue, and centrifuge to obtain a mixed extract A; S2: taking 7-12 parts of Scutellaria baicalensis radix by weight, soaking it in an alkaline solution, washing it with water, drying it, and crushing it, adding 0.6-2.5 parts of hydrogen-type cation exchange resin and 110-160 parts of deionized water thereto, extracting it under reduced pressure and refluxing at a temperature of 60-80° C. for 15-25 minutes, removing the residue, and centrifuging to obtain a mixed extract B; S3: Taking 4-8 parts of carrots by weight, crushing them, adding 80-100 parts of 70% ethanol solution by weight, extracting at 80-100°C for 2-4h, and taking the supernatant by centrifugation; then adding 60-80 parts of 80% ethanol solution by weight, extracting at 85-95°C for 1-3h, combining the supernatants, and centrifuging to obtain a mixed extract C; S4: taking 8-12 parts of grape seeds and 8-14 parts of pomegranate seeds by weight, freeze-drying and crushing them, adding 60-80% polyethylene glycol solvent PEG600 according to a solid-liquid ratio of 1:15-25, extracting at a temperature of 85-95° C. for 1-4 hours, centrifuging the extracted liquid, and filtering the supernatant to obtain a mixed extract D; S5: Mix the mixed extract A described in step S1, the mixed extract B described in step S2, the mixed extract C described in step S3, and the mixed extract D described in step S4, and perform ultrasonic treatment at a temperature of 35-45° C. for 15-25 minutes to obtain the antioxidant plant extract.

[0009] Furthermore, step S1 is specifically as follows: 5 parts of green tea are taken by weight, washed to remove impurities, dried at a temperature of 85°C, crushed to 80-100 mesh, added with deionized water at a solid-liquid ratio of 1:10, refluxed and extracted at 95°C for 3 times, each time for 1 hour, the extracts are combined, the residues are filtered, and the supernatant is taken after centrifugation at a speed of 3000r / min for 15min to obtain a mixed extract A.

[0010] Furthermore, step S2 is specifically as follows: by weight, 10 parts of Scutellaria baicalensis are added with 30 parts of 5% sodium hydroxide solution, soaked at room temperature for 30 minutes, washed with water until neutral, dried at 85°C, and crushed to 100-120 mesh; 2 parts of hydrogen cation exchange resin and 140 parts of deionized water are added thereto, and the mixture is extracted under reduced pressure reflux at 70°C and 0.3MPa for 18 minutes, the residue is removed, and the mixture is centrifuged at 2500r / min for 10 minutes to obtain the supernatant to obtain a mixed extract B.

[0011] Furthermore, step S3 is specifically as follows: by weight, 6 parts of carrots are crushed, 95 parts of 70% ethanol solution are added thereto, extracted at 85°C for 3.5 hours, centrifuged at 1500 r / min for 10 minutes to obtain the supernatant; then 75 parts of 80% ethanol solution are added, extracted at 90°C for 2 hours, the supernatants are combined, and centrifuged at 3500 r / min for 8 minutes to obtain a mixed extract C.

[0012] Furthermore, step S4 is specifically as follows: 10 parts of grape seeds and 12 parts of pomegranate seeds are taken by weight, freeze-dried at a pressure of 4-6Pa and a temperature of -15°C--25°C for 1-2h, cooled to -60°C--70°C at a rate of 5-10°C / min, dried for 2-3h, and crushed to 100-120 mesh; polyethylene glycol solvent PEG600 with a mass concentration of 75% is added thereto according to a solid-liquid ratio of 1:22, extracted at a temperature of 90°C for 2.5h, the extracted liquid is centrifuged at 2000r / min for 10min, and the supernatant is filtered to obtain a mixed extract D.

[0013] Furthermore, the mixture was freeze-dried at a pressure of 5.5 Pa and a temperature of -23°C for 1.5 h, cooled to -65°C at a rate of 8°C / min and dried for 2 h, and then crushed to 100-120 mesh.

[0014] Furthermore, step S5 is specifically: the mixed extract A described in step S1, the mixed extract B described in step S2, the mixed extract C described in step S3, and the mixed extract D described in step S4 are mixed, and ultrasonically treated at a temperature of 42° C. and a power of 2500 W for 18 minutes to obtain the antioxidant plant extract.

[0015] Use of any of the above antioxidant plant extracts in cosmetics.

[0016] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The present invention uses scutellaria baicalensis as a raw material, and after being soaked in a sodium hydroxide solution and treated with a hydrogen-type cation exchange resin, the stability of the extract is enhanced and the extraction rate of antioxidant active substances is improved through the synergistic effect of multiple components; the present invention uses an ethanol solution for secondary extraction of carrots and uses deionized water to extract green tea; the grape seeds and pomegranate seeds are first freeze-dried and then extracted with a polyethylene glycol solvent PEG600, so that the extraction effect of the active ingredients in each component raw material is better, and the extract does not contain irritating substances and has higher safety.

[0017] 2. Under the synergistic effect of the above extracts, the antioxidant effect of the overall plant extracts of the present invention is significantly enhanced, the synergistic effect is enhanced, and the stability is good; when applied to cosmetics, the antioxidant effect is good. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] The hydrogen-type cation exchange resin used in the present invention is produced by Shanghai Myrel Biochemical Technology Co., Ltd., with the product number M79409.

[0020] Example 1 This embodiment provides a method for preparing an antioxidant plant extract, comprising the following steps: S1: Take 3g of green tea, wash and remove impurities, dry at 85℃, crush to 80-100 mesh, add deionized water at a solid-liquid ratio (g / ml) of 1:8; reflux extract at 90℃ for 2 times, each time for 1h, combine the extracted extracts, filter the residue, and centrifuge at 3000r / min for 15min to obtain mixed extract A; S2: Take 7g of Scutellaria baicalensis and add 30g of 5% sodium hydroxide solution, soak at room temperature for 30min, then wash with water until neutral, dry at 85℃, and crush to 100-120 mesh; then add 0.6g of hydrogen cation exchange resin and 110g of deionized water, reflux extract at 60℃ and -0.3MPa for 15min, remove the residue, and centrifuge at 2500r / min for 10min to obtain mixed extract B; S3: 4 g of carrots were crushed, 80 g of 70% ethanol solution was added thereto, the mixture was extracted at 80°C for 2 h, and the mixture was centrifuged at 1500 r / min for 10 min to obtain the supernatant; 60 g of 80% ethanol solution was added thereto, the mixture was extracted at 85°C for 1 h, the supernatants were combined, and the mixture was centrifuged at 3500 r / min for 8 min to obtain a mixed extract C; S4: Take 8g of grape seeds and 8g of pomegranate seeds, freeze-dry them at a pressure of 4Pa and a temperature of -15°C for 1h, cool them to -60°C at a rate of 5°C / min, dry them for 2h, and crush them into 100-120 meshes; add 60% polyethylene glycol solvent PEG600 at a solid-liquid ratio (g / ml) of 1:15, extract them at a temperature of 85°C for 1h, centrifuge the extracted liquid at 2000r / min for 10min, take the supernatant and filter it to obtain a mixed extract D; S5: Mix the mixed extract A described in step S1, the mixed extract B described in step S2, the mixed extract C described in step S3, and the mixed extract D described in step S4, and perform ultrasonic treatment for 15 minutes at a temperature of 35° C. and a power of 2500 W to obtain the antioxidant plant extract.

[0021] Example 2 This embodiment provides a method for preparing an antioxidant plant extract, comprising the following steps: S1: Take 7g of green tea, wash and remove impurities, dry at 85℃, crush to 80-100 mesh, add deionized water at a solid-liquid ratio (g / ml) of 1:12; reflux extract at 100℃ for 4 times, each time for 2h, combine the extracted extracts, filter the residue, and centrifuge at 3000r / min for 15min to obtain mixed extract A; S2: Take 12g of Scutellaria baicalensis and add 30g of 5% sodium hydroxide solution, soak at room temperature for 30min, then wash with water until neutral, dry at 85℃, and crush to 100-120 mesh; then add 2.5g of hydrogen cation exchange resin and 160g of deionized water, reflux extract at 80℃ and -0.3MPa for 25min, remove the residue, and centrifuge at 2500r / min for 10min to obtain mixed extract B; S3: 8 g of carrots were crushed, 100 g of 70% ethanol solution was added thereto, the mixture was extracted at 100°C for 4 h, and the mixture was centrifuged at 1500 r / min for 10 min to obtain the supernatant; 80 g of 80% ethanol solution was added thereto, the mixture was extracted at 95°C for 3 h, the supernatants were combined, and the mixture was centrifuged at 3500 r / min for 8 min to obtain a mixed extract C; S4: Take 12g of grape seeds and 14g of pomegranate seeds, freeze-dry them at a pressure of 6Pa and a temperature of -25°C for 2h, cool them to -70°C at a rate of 10°C / min, dry them for 3h, and crush them into 100-120 meshes; add 80% polyethylene glycol solvent PEG600 at a solid-liquid ratio (g / ml) of 1:25, extract them at a temperature of 95°C for 4h, centrifuge the extracted liquid at 2000r / min for 10min, take the supernatant and filter it to obtain a mixed extract D; S5: Mix the mixed extract A described in step S1, the mixed extract B described in step S2, the mixed extract C described in step S3, and the mixed extract D described in step S4, and perform ultrasonic treatment at a temperature of 45° C. and a power of 2500 W for 25 minutes to obtain the antioxidant plant extract.

[0022] Example 3 This embodiment provides a method for preparing an antioxidant plant extract, comprising the following steps: S1: Take 5g of green tea, wash and remove impurities, dry at 85℃, crush to 80-100 mesh, add deionized water at a solid-liquid ratio of 1:10, reflux extract at 95℃ for 3 times, each extraction for 1h, combine the extracts, filter the residue, centrifuge at 3000r / min for 15min, and take the supernatant to obtain mixed extract A; S2: Take 10g of Scutellaria baicalensis and add 30g of 5% sodium hydroxide solution, soak at room temperature for 30min, then wash with water until neutral, dry at 85℃, and crush to 100-120 mesh; then add 2g of hydrogen cation exchange resin and 140g of deionized water, extract under reduced pressure reflux at 70℃ and 0.3MPa for 18min, remove the residue, centrifuge at 2500r / min for 10min, and take the supernatant to obtain mixed extract B; S3: 6 g of carrots were crushed, 95 g of 70% ethanol solution was added thereto, the mixture was extracted at 85°C for 3.5 h, and the mixture was centrifuged at 1500 r / min for 10 min to obtain the supernatant; 75 g of 80% ethanol solution was added thereto, the mixture was extracted at 90°C for 2 h, the supernatants were combined, and the mixture was centrifuged at 3500 r / min for 8 min to obtain a mixed extract C; S4: Take 10g of grape seeds and 12g of pomegranate seeds, freeze-dry them at a pressure of 5.5Pa and a temperature of -23°C for 1.5h, cool them to -65°C at a rate of 8°C / min, dry them for 2h, and crush them into 100-120 meshes; add 75% polyethylene glycol solvent PEG600 at a solid-liquid ratio of 1:22, extract them at a temperature of 90°C for 2.5h, centrifuge the extracted liquid at 2000r / min for 10min, take the supernatant and filter it to obtain a mixed extract D; S5: The mixed extract A described in step S1, the mixed extract B described in step S2, the mixed extract C described in step S3, and the mixed extract D described in step S4 are mixed, and ultrasonically treated at a temperature of 42° C. and a power of 2500 W for 18 minutes to obtain the antioxidant plant extract.

[0023] Comparative Example 1 The difference between this comparative example and Example 1 is that step S1 is missing, and the other steps are the same.

[0024] Comparative Example 2 The difference between this comparative example and Example 1 is that step S2 is missing, and the other steps are the same.

[0025] Comparative Example 3 The difference between this comparative example and Example 1 is that step S3 is missing, and the other steps are the same.

[0026] Comparative Example 4 The difference between this comparative example and Example 1 is that step S4 is missing, and the other steps are the same.

[0027] Comparative Example 5 The difference between this comparative example and Example 1 is that in step S2, water extraction is directly adopted, that is, 7 g of Scutellaria baicalensis is added to 30 g of deionized water solution, soaked at room temperature for 30 min, dried at a temperature of 85° C., and crushed to 100-120 mesh; then 110 g of deionized water is added thereto, refluxed and extracted at a temperature of 60° C. and -0.3 MPa for 15 min, the residue is removed, and the mixed extract B is obtained by centrifugation at a speed of 2500 r / min for 10 min; the other steps are the same.

[0028] Comparative Example 6 The difference between this comparative example and Example 1 is that in step S4, freeze-drying is not performed, that is, 8 g of grape seeds and 8 g of pomegranate seeds are taken, and polyethylene glycol solvent PEG600 with a mass concentration of 60% is added thereto according to a solid-liquid ratio (g / ml) of 1:15, and the mixture is extracted at a temperature of 85°C for 1 h, the extracted liquid is centrifuged at 2000 r / min for 10 min, and the supernatant is filtered to obtain a mixed extract D; the other steps are the same.

[0029] Application in cosmetics The antioxidant plant extracts obtained in the embodiments and comparative examples are applied to cosmetics. 1% of the antioxidant plant extract, 1% of alcohol, 1% of glycerin, 1% of methylcellulose and 1% of cyclopentasiloxane are added to water, and stirred at 80°C until there is no solid insoluble matter; 0.01% of xanthan gum and 1% of polyoxyethylene-2 ​​stearyl ether are added in sequence, and mixed thoroughly, keeping the temperature constant, and fully emulsifying to a uniform and stable cream state; 0.01% of fragrance and 0.02% of imidazolidinyl urea are added, mixed and stirred, and the solution is cooled to room temperature, and 0.01% of hyaluronic acid and 0.1% of ethylhexyl palmitate are added, and stirred evenly to obtain antioxidant cosmetics.

[0030] Performance Test: Evaluation of DPPH antioxidant effect: Take 2.5mL of the antioxidant cosmetics prepared by the embodiment and comparative example, add it to 2.5mL of 65μmol / L DPPH solution, stir evenly, leave it for 40min, and measure the absorbance at 517nm as Ai; in the same way, take 2.5mL of anhydrous ethanol and add it to 2.5mL of DPPH solution and mix evenly, and measure the absorbance as Ac; take another 2.5mL of the embodiment and comparative example products and add them to 2.5mL of anhydrous ethanol, mix evenly, and measure the absorbance as Aj. Calculate its free radical scavenging rate according to the following formula. Scavenging rate (%) = [1-(Ai-Aj) / Ac]×100%; where Aj is the contribution of the embodiment and comparative example to the absorbance; Ai is the absorbance value of the embodiment and comparative example after the action of DPPH; Ac is the absorption of DPPH itself at the measured wavelength.

[0031] Centrifugal stability test: Take 3 groups of 30 mL samples of the example in a centrifuge, keep the speed at 2500 r / min for 10 min, take out, and compare whether the appearance and smell of the samples have changed; High temperature stability test: 3 groups of 30 mL samples of the example were placed in a 42°C oven for 1 month, then taken out and placed at room temperature for 24 hours to return to room temperature, and the appearance and smell of the samples were compared for changes. The results are shown in the following table: Table 1 Performance test results

[0032] From the above performance test results, it can be seen that the free radical scavenging rate and stability of Examples 1-3 are good and the effects are obvious. In particular, the comprehensive performance of Example 3 is the most outstanding.

[0033] However, the comparative example does not adopt the necessary technical solution, resulting in its performance being significantly worse than that of the embodiment in the corresponding performance test. The above experimental results further prove the importance of the technical solution defined in the present invention to its technical effect.

[0034] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A plant extract having antioxidant properties, characterized in that: The invention comprises the following components by weight: 4-8 parts of carrots, 7-12 parts of scutellaria baicalensis, 8-12 parts of grape seeds, 8-14 parts of pomegranate seeds and 3-7 parts of green tea.

2. The antioxidant plant extract according to claim 1, characterized in that: The components are further optimized as follows: 6 parts of carrots, 10 parts of scutellaria baicalensis, 10 parts of grape seeds, 12 parts of pomegranate seeds, and 5 parts of green tea.

3. A method for preparing an antioxidant plant extract, characterized in that: The steps include: S1: Wash, dry and crush 3-7 parts of green tea by weight, add deionized water at a solid-liquid ratio of 1:8-12, reflux and extract at 90-100°C for 2-4 times, each time for 1-2 hours, combine the extracted extracts, filter the residue, and centrifuge to obtain a mixed extract A; S2: taking 7-12 parts of Scutellaria baicalensis by weight, soaking it in an alkaline solution, washing it with water, drying it, and crushing it, adding 0.6-2.5 parts of hydrogen-type cation exchange resin and 110-160 parts of deionized water thereto, extracting it under reduced pressure and refluxing at a temperature of 60-80°C for 15-25 minutes, removing the residue, and mixing it by centrifugation to obtain extract B; S3: Taking 4-8 parts of carrots by weight, crushing them, adding 80-100 parts of 70% ethanol solution by weight, extracting at 80-100°C for 2-4h, and taking the supernatant by centrifugation; then adding 60-80 parts of 80% ethanol solution by weight, extracting at 85-95°C for 1-3h, combining the supernatants, and centrifuging to obtain a mixed extract C; S4: taking 8-12 parts of grape seeds and 8-14 parts of pomegranate seeds by weight, freeze-drying and crushing them, adding 60-80% polyethylene glycol solvent PEG600 according to a solid-liquid ratio of 1:15-25, extracting at a temperature of 85-95° C. for 1-4 hours, centrifuging the extracted liquid, and filtering the supernatant to obtain a mixed extract D; S5: Mix the mixed extract A described in step S1, the mixed extract B described in step S2, the mixed extract C described in step S3, and the mixed extract D described in step S4, and perform ultrasonic treatment at a temperature of 35-45° C. for 15-25 minutes to obtain the antioxidant plant extract.

4. The method for preparing an antioxidant plant extract according to claim 3, characterized in that: Step S1 is specifically as follows: 5 parts of green tea are washed to remove impurities, dried at 85°C, crushed to 80-100 mesh, added with deionized water at a solid-liquid ratio of 1:10, and extracted 3 times at 95°C under reflux, each time for 1 hour, the extracts are combined, the residue is filtered, and the mixed extract A is obtained after centrifugation at 3000r / min for 15 minutes.

5. The method for preparing an antioxidant plant extract according to claim 3, characterized in that: Step S2 is specifically as follows: by weight, 10 parts of Scutellaria baicalensis are added with 30 parts of 5% sodium hydroxide solution, soaked at room temperature for 30 minutes, washed with water until neutral, dried at 85°C, and crushed to 100-120 mesh; 2 parts of hydrogen cation exchange resin and 140 parts of deionized water are added thereto, and the mixture is extracted under reduced pressure reflux at 70°C and 0.3MPa for 18 minutes, the residue is removed, and the mixture is centrifuged at 2500r / min for 10 minutes to obtain the supernatant to obtain a mixed extract B.

6. The method for preparing an antioxidant plant extract according to claim 3, characterized in that: Step S3 is specifically as follows: by weight, 6 parts of carrots are crushed, 95 parts of 70% ethanol solution are added thereto, the mixture is extracted at 85°C for 3.5 hours, and the mixture is centrifuged at 1500 r / min for 10 minutes to obtain the supernatant; then 75 parts of 80% ethanol solution are added, the mixture is extracted at 90°C for 2 hours, the supernatants are combined, and the mixture is centrifuged at 3500 r / min for 8 minutes to obtain a mixed extract C.

7. The method for preparing an antioxidant plant extract according to claim 3, characterized in that: Step S4 is specifically as follows: taking 10 parts of grape seeds and 12 parts of pomegranate seeds by weight, freeze-drying them at a pressure of 4-6 Pa and a temperature of -15°C--25°C for 1-2 hours, cooling them to -60°C--70°C at a rate of 5-10°C / min, drying them for 2-3 hours, and crushing them into 100-120 meshes; adding 75% polyethylene glycol solvent PEG600 at a solid-liquid ratio of 1:22, extracting them at a temperature of 90°C for 2.5 hours, centrifuging the extracted liquid at 2000r / min for 10 minutes, and filtering the supernatant to obtain a mixed extract D.

8. The method for preparing an antioxidant plant extract according to claim 7, characterized in that: Freeze-dry at a pressure of 5.5 Pa and a temperature of -23°C for 1.5 h, cool to -65°C at a rate of 8°C / min, and dry for 2 h, then crush to 100-120 mesh.

9. The method for preparing an antioxidant plant extract according to claim 3, characterized in that: Step S5 is specifically as follows: the mixed extract A described in step S1, the mixed extract B described in step S2, the mixed extract C described in step S3, and the mixed extract D described in step S4 are mixed, and ultrasonically treated for 18 minutes at a temperature of 42° C. and a power of 2500 W to obtain the antioxidant plant extract.

10. Use of the antioxidant plant extract according to any one of claims 1 to 9 in cosmetics.

Images