Chromatography device, chromatography system and method for replacing a chromatographic column

Abstract

The present application relates to a chromatographic device, a chromatographic system and a method for replacing a chromatographic column. The chromatographic device includes a chromatographic cassette and a quick-insert and quick-replace mechanism. The chromatographic cassette includes a cassette body and a chromatographic column disposed in the cassette body. Both ends of the chromatographic column are connected to a transfer assembly passing through the side wall of the cassette body. The transfer assembly is provided with a first channel communicating with the chromatographic column. The quick-insert and quick-replace mechanism includes a bracket and a sample inlet / outlet unit disposed on the bracket. The bracket is provided with a positioning assembly for positioning the chromatographic cassette. The sample inlet / outlet unit includes two movable sample needles, and the two sample needles are used to be respectively connected to the two transfer assemblies. When the chromatographic cassette is positioned on the bracket, the sample needles can move between a first position and a second position; when the sample needles move to the first position, the sample needles are inserted into the first channels of the transfer assemblies so that the sample needles communicate with the first channels; when the sample needles move to the second position, the sample needles are separated from the transfer assemblies. The present application quickly replaces the chromatographic column from the chromatographic device by replacing the chromatographic cassette.

Description

Technical Field

[0001] This application relates to the technical field of analytical and purification equipment, and particularly to a chromatographic device, a chromatographic system, and a method for replacing a chromatographic column. Background Art

[0002] Chromatographic analysis is widely used in many fields such as chemistry, biochemistry, pharmaceuticals, food science, and environmental science for the analysis and purification of complex components in samples. For example: in drug research and development, it can be used for drug purification; in environmental detection, it can be used to detect pollutants in water and air; in food science, it can be used to analyze food ingredients.

[0003] High-performance liquid chromatography (HPLC) is an important branch of chromatographic analysis, and the chromatographic column is a key part in the HPLC system for separating each component in the sample. The chromatographic column usually consists of a slender columnar container filled with a stationary phase (such as silica gel, polymer, etc.). When the mobile phase containing the sample (such as solvent, water, etc.) passes through the stationary phase, the stationary phase will interact with each component in the sample, thereby realizing the separation of each component in the sample. The performance of the chromatographic column directly affects the analysis results and repeatability. However, as the usage time of the chromatographic column increases, problems such as column blockage, chemical degradation, physical wear, and matrix effects may occur in the chromatographic column, thus requiring the replacement of a new chromatographic column; and when chromatographic analysis needs to frequently switch samples, a new chromatographic column is also required.

[0004] Currently, the methods for replacing chromatographic columns mainly include the following several:

[0005] (1) Direct replacement method, that is, unscrew the connectors at both ends of the fixed chromatographic column, remove the old chromatographic column from the HPLC system, then install the new chromatographic column in the same position, and then tighten the connectors used to fix both ends of the chromatographic column again. The direct replacement method is not only inconvenient to operate, but also may cause an instantaneous increase in the mobile phase pressure in the HPLC system during the replacement process, thereby causing an impact on the components in the HPLC system (such as pumps, connectors, etc.); in addition, air may be introduced during the replacement process, resulting in changes in the solvent strength and pH value of the mobile phase, and further affecting the accuracy of the analysis results.

[0006] (2) On-line replacement method, that is, through the on-line replacement interface of the HPLC system, directly switch the old chromatographic column to the new chromatographic column while keeping the HPLC system running. The on-line replacement method has a high cost and complex operation, and requires professional personnel to operate. In addition, during the process of on-line replacing the chromatographic column, the switching between the old and new chromatographic columns may cause a short-term fluctuation in the analysis results, and further affect the accuracy of the analysis results.

[0007] (3) Chromatographic column regeneration method, that is, using specific chemical reagents or physical methods to regenerate a blocked or degraded chromatographic column to restore its performance. The regeneration process of the chromatographic column is relatively complex and requires professional chemical knowledge and equipment; moreover, the performance of the regenerated chromatographic column cannot be fully restored to the initial state, affecting the long-term stability and accuracy of the analysis results.

[0008] (4) Spare chromatographic column method, that is, installing a spare chromatographic column in the HPLC system. When the main chromatographic column needs to be replaced, only switch to the spare chromatographic column. This method requires a spare chromatographic column and an HPLC system capable of automatically switching chromatographic columns, and the spare chromatographic column needs to be regularly maintained and calibrated. This method increases the complexity and cost of the HPLC system. In addition, the long-term non-use of the spare chromatographic column may cause its performance to decline, thus affecting the accuracy of the analysis results. Summary of the Invention

[0009] Based on this, it is necessary to provide a chromatographic device, a chromatographic system and a method for replacing a chromatographic column that can replace the chromatographic column simply and quickly.

[0010] The chromatographic device includes a chromatographic cassette and a quick-insert and quick-change mechanism. The chromatographic cassette includes a cassette body and a chromatographic column disposed in the cassette body. Both ends of the chromatographic column are connected with adapter assemblies. Both of the adapter assemblies penetrate through the side wall of the cassette body. The adapter assembly is provided with a first channel, and the first channel is communicated with the inside of the chromatographic column. The quick-insert and quick-change mechanism includes a bracket and a sampling unit disposed on the bracket. The bracket is provided with a positioning component for positioning the chromatographic cassette. The sampling unit includes two movable sample needles, and the two sample needles are used to be respectively connected with the two adapter assemblies. When the chromatographic cassette is positioned on the bracket by the positioning component, the sample needles can move between a first position and a second position. When the sample needles move to the first position, the sample needles are inserted into the first channels of the adapter assemblies so that the inside of the sample needles is communicated with the first channels. When the sample needles move to the second position, the sample needles are separated from the adapter assemblies.

[0011] In one embodiment, the quick-insert and quick-change mechanism further includes a pressing component, and the pressing component includes a pressing member, a connecting member and a first elastic member.

[0012] One end of the connecting member is rotatably connected to the bracket, and the other end of the connecting member is connected to the pressing member. The first elastic member acts on the connecting member or the pressing member to make the pressing member press against the chromatographic cassette.

[0013] In one embodiment, both ends of the adapter assembly are respectively disposed on both sides of the side wall of the cassette body;

[0014] The transfer component includes a seal, a seal mounting seat, and a seal mounting cover. The seal mounting seat penetrates through the side wall of the box body. The seal mounting seat is detachably connected to the seal mounting cover, and the seal mounting seat and the seal mounting cover enclose a sealed space. The seal is disposed in the sealed space, and the seal is provided with the first channel;

[0015] When the sample needle is in the first position, the sample needle penetrates through the seal mounting cover, and the outer wall of the sample needle is in tight fit with the first channel.

[0016] In one embodiment, the transfer component further includes a seal pressing ring. The seal pressing ring is disposed between the seal and the seal mounting cover to press the seal against the seal mounting seat.

[0017] In one embodiment, a heating sub-box is provided in the chromatography box. A pressing plate and a heating component in tight fit are provided in the heating sub-box. A fixing groove is defined between the pressing plate and the heating component. The fixing groove is used to accommodate at least part of the chromatography column. The heating component is used to heat the chromatography column accommodated in the fixing groove.

[0018] In one embodiment, the chromatography column is connected to the transfer component through a pipeline. The pipeline includes a sampling pipeline. One end of the sampling pipeline is communicated with the first channel of one of the transfer components, and the other end of the sampling pipeline is connected to the sampling port of the chromatography column. The fixing groove is further used to accommodate part of the sampling pipeline. The heating component is further used to heat part of the sampling pipeline accommodated in the fixing groove.

[0019] In one embodiment, the heating component includes a heating element, a heat conducting element, a temperature sensor, and a temperature protection switch. One side of the heat conducting element is connected to the heating element, and the other side of the heat conducting element is in tight fit with the pressing plate. The fixing groove is defined between the pressing plate and the heat conducting element.

[0020] The heating element is used to heat the chromatography column accommodated in the fixing groove;

[0021] The temperature sensor is used to detect the temperature of the heat conducting element;

[0022] The temperature sensor and the heating element are both communicatively connected to the temperature protection switch. The temperature protection switch is used to turn on or off the heating element according to the temperature detected by the temperature sensor.

[0023] In one embodiment, a socket is further provided in the box body. An intelligent chip is provided in the socket. The socket penetrates through the box body;

[0024] The bracket is provided with a plug-in component. When the chromatographic cartridge is positioned on the bracket by the positioning component, the plug-in component is inserted into the plug-in socket.

[0025] The present application also provides a chromatographic system, including the chromatographic device described in any one of the above embodiments.

[0026] The present application also provides a method for replacing a chromatographic column. The replacement method is used to replace the chromatographic column in the chromatographic device in any one of the above embodiments. The chromatographic cartridge includes a first chromatographic cartridge and a second chromatographic cartridge. The first chromatographic cartridge is the chromatographic cartridge positioned on the bracket of the chromatographic device before replacing the chromatographic column, and the second chromatographic cartridge is the chromatographic cartridge positioned on the bracket of the chromatographic device after replacing the chromatographic column.

[0027] The replacement method includes the following steps:

[0028] The sample needle moves from the first position to the second position, and the sample needle is separated from the transfer component of the first chromatographic cartridge.

[0029] Take out the first chromatographic cartridge from the bracket.

[0030] Position the second chromatographic cartridge on the bracket.

[0031] The sample needle moves from the second position to the first position, and the sample needle is inserted into the first channel of the transfer component of the second chromatographic cartridge. The sample needle is in communication with the first channel of the second chromatographic cartridge, thereby completing the replacement of the chromatographic column.

[0032] Compared with the prior art, the chromatographic device provided by the present application includes a chromatographic cartridge and a quick-insert and quick-replace mechanism. The quick-insert and quick-replace mechanism includes a bracket and a sampling unit provided on the bracket. In the present application, the chromatographic column is integrated into the box body, and the transfer components connected to both ends of the chromatographic column are arranged on the box body. When the chromatographic column needs to be replaced, only the entire chromatographic cartridge needs to be replaced. Since the bracket is provided with positioning parts, the chromatographic cartridge is positioned by the positioning parts. Then, by moving the sample needle, when the sample needle moves to the first position, the inside of the sample needle is in communication with the first channel. Since the first channel is in communication with the inside of the chromatographic column, the inside of the sample needle and the inside of the chromatographic column can be communicated, thus realizing the installation of the chromatographic cartridge. When the sample needle moves to the second position, the sample needle is separated from the transfer component, so that the chromatographic cartridge can be removed from the bracket, thus realizing the quick disassembly of the chromatographic cartridge. It can be seen that in the present application, by integrating the chromatographic column and the box body into a whole and providing a movable sample needle on the bracket, the quick replacement of the chromatographic cartridge can be realized. Description of the Drawings

[0033] To more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0034] Figure 1 Stereogram of a chromatographic device according to an embodiment of the present application;

[0035] Figure 2 Schematic diagram of the internal structure of a chromatographic cartridge according to an embodiment of the present application;

[0036] Figure 3 Cross-sectional view of a chromatographic cartridge according to an embodiment of the present application;

[0037] Figure 4 For Figure 3 Partial enlarged view at A in

[0038] Figure 5 Schematic diagram of a quick-insert and quick-change mechanism according to an embodiment of the present application;

[0039] Figure 6 Schematic diagram of another perspective of a quick-insert and quick-change mechanism according to an embodiment of the present application.

[0040] Reference numerals:

[0041] 10, chromatographic cartridge; 110, cartridge body; 111, guide groove; 112, limiting member; 113, socket; 114, handle;

[0042] 120, chromatographic column; 121, sampling pipeline;

[0043] 130, adapter assembly; 131, first channel; 132, seal; 133, seal mounting seat; 134, seal mounting cover; 135, seal pressing ring; 136, first avoidance hole; 137, second avoidance hole; 138, third avoidance hole;

[0044] 140, heating sub-cartridge; 141, bottom plate; 142, pressing plate; 143, heating assembly; 1431, fixing groove; 1432, heating element; 1433, heat conducting member; 1434, temperature sensor; 1435, temperature protection switch; 144, cover;

[0045] 20, quick-insert and quick-change mechanism;

[0046] 210, bracket; 211, first position detection member; 212, plug-in member;

[0047] 220. Sample inlet and outlet unit; 221. Sample needle; 222. Pushing component; 2221. Fixed plate; 2222. Pressing plate; 2223. Second elastic member; 2224. Guide shaft; 2225. Stopping portion; 2226. Groove; 223. Driving component; 2231. Driving member; 2232. Lead screw; 2233. Slide block; 2234. First guide rail; 2235. Transmission group; 224. Mounting seat; 225. Second position detecting member;

[0048] 230. Positioning component; 231. Positioning groove; 232. First opening; 233. Second opening; 234. Positioning plate; 2341. First flanging; 2342. Second flanging; 2343. Third flanging; 235. First plate; 236. Guide sliding rail;

[0049] 240. Pressing component; 241. Pressing member; 242. Connecting member; 243. First elastic member; 244. Shock pad. Detailed implementation manners

[0050] To make the above objects, features, and advantages of the present application more obvious and understandable, the following will describe the detailed implementation manners of the present application with reference to the accompanying drawings. Many specific details are set forth in the following description to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein. Those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.

[0051] It should be noted that when a component is referred to as being "fixed to" or "disposed on" another component, it can be directly on the other component or there may also be an intermediate component. When a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be an intermediate component at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in the description of the present application are only for the purpose of illustration and do not represent the only implementation manner.

[0052] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0053] In this application, unless otherwise clearly specified and defined, the first feature being "on" or "under" the second feature may mean that the first feature is in direct contact with the second feature, or the first feature is in indirect contact with the second feature through an intermediate medium. Moreover, the first feature being "above", "over" or "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" or "underneath" the second feature may mean that the first feature is directly below or obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0054] Unless otherwise defined, all technical and scientific terms used in the description of this application have the same meaning as commonly understood by those skilled in the technical field to which this application belongs. The terms used in the description of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used in the description of this application includes any and all combinations of one or more of the related listed items.

[0055] Please refer to Figures 1 to 6 , this application provides a chromatography device, including a chromatography cartridge 10 and a quick-insert and quick-change mechanism 20. The chromatography cartridge 10 includes a cartridge body 110 and a chromatography column 120 disposed inside the cartridge body 110. Both ends of the chromatography column 120 are connected with an adapter assembly 130. Both adapter assemblies 130 penetrate through the side wall of the cartridge body 110. The adapter assembly 130 is provided with a first channel 131, and the first channel 131 is in internal communication with the chromatography column 120. The quick-insert and quick-change mechanism 20 includes a bracket 210 and a sample inlet / outlet unit 220 disposed on the bracket 210. The bracket 210 is provided with a positioning assembly 230 for positioning the chromatography cartridge 10. The sample inlet / outlet unit 220 includes two movable sample needles 221 for respectively connecting with the two adapter assemblies 130. When the chromatography cartridge 10 is positioned on the bracket 210 by the positioning assembly 230, the sample needles 221 can move between a first position and a second position. When the sample needles 221 move to the first position, the sample needles 221 are inserted into the first channel 131 of the adapter assembly 130 to make the inside of the sample needles 221 communicate with the first channel 131. When the sample needles 221 move to the second position, the sample needles 221 are separated from the adapter assembly 130.

[0056] In this embodiment, the adapter assembly 130 of the sample needle 221 for connecting the chromatographic column 120 and the quick-insert and quick-change mechanism 20 is inserted through the side wall of the box body 110 of the chromatographic cassette 10, and then the chromatographic column 120 is integrated into the box body 110 of the chromatographic cassette 10. When it is necessary to replace the chromatographic column 120 in the chromatographic device, the chromatographic cassette 10 with the old chromatographic column is directly removed, and another chromatographic cassette 10 with a new chromatographic column is installed, thus completing the replacement of the chromatographic column 120. That is to say, in this application, the chromatographic column 120 and the box body 110 are integrated into a whole, and a sample needle 221 that can move between a first position and a second position is provided on the bracket 210, so that the sample needle 221 is connected or disconnected from the chromatographic column 120 inside the chromatographic cassette 10 through the first channel 131 of the adapter assembly 130, and the adapter assembly 130 is inserted through the side wall of the box body 110 of the chromatographic cassette 10. In this embodiment, the quick replacement of the chromatographic column 120 can be completed by replacing the chromatographic cassette 10.

[0057] It can be understood that before installing the chromatographic cassette 10 with a new chromatographic column into the chromatographic device, it is necessary to install the new chromatographic column in the chromatographic cassette 10 in advance and connect the new chromatographic column to the adapter assembly 130. Because when preparing the chromatographic cassette 10 with a new chromatographic column, it is not necessary to pause the operation of the chromatographic device, and it can be prepared before removing the chromatographic cassette 10 with the old chromatographic column. For the chromatographic device, when the sample needle 221 is moved to the second position, after the sample needle 221 is separated from the adapter assembly 130, the chromatographic cassette 10 with the old chromatographic column is directly taken out, and then the chromatographic cassette 10 with the new chromatographic column is placed at the target position. When the sample needle 221 is moved to the first position, the inside of the sample needle 221 is connected to the first channel 131. In this way, the quick replacement of the chromatographic cassette 10 and the chromatographic column 120 is realized, and using the chromatographic device in this embodiment can simplify the process of replacing the chromatographic column 120 and save the time for replacing the chromatographic column 120.

[0058] Furthermore, both ends of the chromatographic column 120 are connected to the adapter assembly 130 through pipelines and are connected to the first channel 131 through the pipelines. In this way, the installation and removal of the chromatographic column 120 in the chromatographic cassette 10 can be facilitated, and the universality of the chromatographic column 120 for the chromatographic cassette 10 can be improved.

[0059] It should also be noted that when the sample needle 221 is not inserted into the first channel 131 of the adapter assembly 130, the first channel 131 is airtight. In this way, when the sample needle 221 is connected to the chromatographic column 120 through the first channel 131, it can be ensured that the sample will not leak in the adapter assembly 130 during the process of the sample flowing into the chromatographic column 120.

[0060] The chromatographic device is used as follows: Place the chromatographic cartridge 10 on the support 210. The positioning component 230 on the support 210 positions the chromatographic cartridge 10. After the chromatographic cartridge 10 reaches the target position, when the sample needle 221 is moved to the first position, the inside of the sample needle 221 is in communication with the inside of the chromatographic column 120 through the first channel 131 of the transfer component 130, thus realizing the installation of the chromatographic column 120 on the chromatographic device. If there is a chromatographic cartridge 10 with an old chromatographic column on the support 210 of the quick plug-and-play mechanism 20, it is necessary to first disassemble the chromatographic cartridge 10 with the old chromatographic column. The disassembly method is as follows: When the sample needle 221 is moved to the second position, the sample needle 221 is moved away from the transfer component 130, and the sample needle 221 is disconnected from the chromatographic column 120 inside the chromatographic cartridge 10, and then the chromatographic cartridge 10 with the old chromatographic column can be directly taken out from the support 210; if there is no chromatographic cartridge 10 with an old chromatographic column on the support 210, that is, it is not necessary to first remove the chromatographic cartridge 10 with the old chromatographic column, then the chromatographic cartridge 10 with the new chromatographic column can be directly installed on the chromatographic device.

[0061] Further, in this embodiment, the sample needle 221 can also be moved between the first position and the second position in an automated manner. Schematically, the positioning component 230 is communicatively connected to a programmable logic controller (PLC). The positioning component 230 detects the position of the chromatographic cartridge 10 in real time and uploads it to the PLC. After the positioning component 230 or the PLC determines that the chromatographic cartridge 10 has reached the target position, the PLC controls the driving member 2231 (such as a motor or a cylinder) to move the sample needle 221 from the second position to the first position, and the sample needle 221 is in communication with the chromatographic column 120 through the first channel 131 of the transfer component 130. Similarly, after the PLC receives the instruction that the sample needle 221 needs to move from the second position to the first position, the PLC controls the driving member 2231 to move the sample needle 221 from the first position to the second position, and the sample needle 221 is disconnected from the chromatographic column 120, and the sample needle 221 is separated from the first channel 131 of the transfer component 130. In other embodiments, the sample needle 221 can also be moved between the first position and the second position manually, and the present application does not limit this.

[0062] It should be explained that the target position is the position where the sample needle 221 can enter the first channel 131 of the transfer component 130 when the sample needle 221 is in the first position. The present application does not limit the specific position of the target position on the quick plug-and-play mechanism 20.

[0063] In one embodiment, refer to Figure 2 、 Figure 3 and Figure 4, both ends of the transfer component 130 are respectively arranged on both sides of the side wall of the box body 110; the transfer component 130 includes a seal 132, a seal mounting seat 133 and a seal mounting cover 134. The seal mounting seat 133 penetrates through the side wall of the box body 110. The seal mounting seat 133 is detachably connected to the seal mounting cover 134, and the seal mounting seat 133 and the seal mounting cover 134 enclose a sealed space. The seal 132 is arranged in the sealed space, and the seal 132 is provided with a first channel 131. When the sample needle 221 is in the first position, the sample needle 221 penetrates through the seal mounting cover 134, and the outer wall of the sample needle 221 is in tight fit with the first channel 131. Even if the chromatographic column 120 is placed in the chromatographic box 10, the sample can enter the chromatographic column 120 through the sample needle 221 and the transfer component 130, and leave the chromatographic column 120 through another sample needle 221 and the transfer component 130. In this way, the chromatographic column 120 can be replaced by replacing the chromatographic box 10 in this embodiment. Moreover, when the sample needle 221 is connected to the chromatographic column 120 through the first channel 131, it can ensure that the sample will not leak in the transfer component 130 during the process of flowing into the chromatographic column 120, thereby ensuring the normal operation of the subsequent chromatographic device.

[0064] It can be understood that the seal mounting cover 134 is provided with a first avoidance hole 136, and the first avoidance hole 136 is arranged corresponding to the first channel 131. The sample needle 221 penetrates through the seal mounting cover 134 through the first avoidance hole 136. During the process of the sample needle 221 moving from the second position to the first position and inserting into the first channel 131, the sample needle 221 needs to pass through the first avoidance hole 136 before inserting into the first channel 131.

[0065] It can also be understood that the seal mounting seat 133 is provided with a second avoidance hole 137, and the second avoidance hole 137 is arranged corresponding to the first channel 131. The pipeline connected to the chromatographic column 120 penetrates through the seal mounting seat 133 through the second avoidance hole 137. When the pipeline is communicated with the first channel 131, the pipeline needs to pass through the second avoidance hole 137 before inserting into the first channel 131 and being communicated with the first channel 131.

[0066] Furthermore, the central axis of the first avoidance hole 136, the central axis of the first channel 131 and the central axis of the second avoidance hole 137 are coaxial. In this way, when the sample needle 221 is in the first position, it can ensure the smooth flow of the sample through the sample needle 221 and the transfer component 130.

[0067] In one embodiment, the adapter assembly 130 further includes a sealing pressure ring 135, which is disposed between the sealing member 132 and the sealing mounting cover 134 to press the sealing member 132 against the sealing mounting seat 133. In this way, the position of the sealing member 132 can be stabilized to prevent the sealing member 132 from moving or deviating in the sealed space enclosed by the sealing mounting seat 133 and the sealing mounting cover 134, thereby ensuring that the central axis of the first channel 131 remains unchanged, so that the sample needle 221 can be accurately inserted into the first channel 131 each time it reaches the first position.

[0068] It can be understood that the third avoidance hole 138 is provided on the sealing pressure ring 135, the first avoidance hole 136, the third avoidance hole 138 and the first channel 131 are correspondingly provided, and the sample needle 221 is inserted into the sealing pressure ring 135 through the third avoidance hole 138. In the process of the sample needle 221 moving from the second position to the first position and inserting into the first channel 131, the sample needle 221 needs to first pass through the first avoidance hole 136 and the third avoidance hole 138 before being inserted into the first channel 131.

[0069] In one embodiment, see Figure 2 and Figure 3 , a heating sub-box 140 is provided in the chromatographic box 10, a pressing plate 142 and a heating assembly 143 which are tightly fitted are provided in the heating sub-box 140, a fixing groove 1431 is provided between the pressing plate 142 and the heating assembly 143, the fixing groove 1431 is used to accommodate at least part of the chromatographic column 120, and the heating assembly 143 is used to heat the chromatographic column 120 accommodated in the fixing groove 1431. There is only one heating sub-box 140 in each chromatographic box 10, and only one chromatographic column 120 is provided in each heating sub-box 140. By providing the fixing groove 1431 between the pressing plate 142 and the heating assembly 143 as the temperature control space of the chromatographic column 120, and only one chromatographic column 120 is provided in each fixing groove 1431, the overall size of the heating assembly 143 is greatly reduced, and the temperature control space is made more compact. At the same time, a heating component 143 controls the temperature of a chromatographic column 120. When multiple chromatographic devices are used at the same time, each chromatographic column 120 can be individually temperature controlled. Since the chromatographic columns 120 are independent of each other, the temperature control of each chromatographic column 120 is more precise, avoiding temperature interference between multiple chromatographic columns 120, and making the chromatographic device's detection results of samples more stable and reliable.

[0070] In one embodiment, the chromatographic column 120 is connected through a pipeline and a transfer assembly 130. The pipeline includes an injection pipeline 121. One end of the injection pipeline 121 communicates with the first channel 131 of one of the transfer assemblies 130, and the other end of the injection pipeline 121 is connected to the injection port of the chromatographic column 120. The fixing groove 1431 is further used to accommodate a part of the injection pipeline 121, and the heating assembly 143 is further used to heat the part of the injection pipeline 121 accommodated in the fixing groove 1431 to preheat the sample in the injection pipeline 121. In this way, the temperature of the sample in the chromatographic column 120 can reach the target temperature faster, and the temperature of the sample in the chromatographic column 120 is more accurate, so that the detection result of the chromatographic device for the sample is more stable and reliable.

[0071] In one embodiment, the heating assembly 143 includes a heating element 1432 and a heat conducting element 1433. One side of the heat conducting element 1433 is connected to the heating element 1432, and the other side of the heat conducting element 1433 is in tight fit with the pressing plate 142. A fixing groove 1431 is defined between the pressing plate 142 and the heat conducting element 1433. The heating element 1432 is used to heat the chromatographic column 120 and a part of the injection pipeline 121 accommodated in the fixing groove 1431. It can be understood that after the heating element 1432 generates heat, the heat is transferred to the heat conducting element 1433, and then the heat conducting element 1433 transfers the heat to the temperature control space of the fixing groove 1431, so as to heat the chromatographic column 120 and a part of the injection pipeline 121 in the fixing groove 1431.

[0072] In one embodiment, the heating assembly 143 further includes a temperature sensor 1434 and a temperature protection switch 1435. The temperature sensor 1434 is used to detect the temperature of the heat conducting element 1433; both the temperature sensor 1434 and the heating element 1432 are communicatively connected to the temperature protection switch 1435, and the temperature protection switch 1435 is used to turn on or off the heating element 1432 according to the temperature detected by the temperature sensor 1434. In this way, precise temperature control of the chromatographic column 120 in the heating sub-box 140 can be achieved.

[0073] Further, the heating sub-box 140 includes a bottom plate 141 and a cover 144. The bottom plate 141 and the cover 144 define an installation space, and the chromatographic column 120, the heating assembly 143 and the pressing plate 142 are all arranged in this installation space. And the heating assembly 143 is arranged on the bottom plate 141, the bottom plate 141 is arranged on the side of the heating element 1432 away from the heat conducting element 1433, and the bottom plate 141 and the cover 144 are detachably connected. After the cover 144 is removed from the bottom plate 141, the chromatographic column 120 can be replaced. Integrating the chromatographic column 120 and the heating assembly 143 in the heating sub-box 140, in this way, the setting of the heating sub-box 140 is more compact, saving the volume of the heating sub-box 140 and the chromatographic box 10.

[0074] It should be noted that the steps for replacing the chromatographic column 120 from the heating sub-box 140 are as follows: Remove the cover 144, remove the pressing plate 142, disconnect the connections between the two ends of the chromatographic column 120 and the pipelines, connect the two ends of the new chromatographic column 120 to the pipelines, place the chromatographic column 120 into the fixing groove 1431, and after connecting the pressing plate 142, install the cover 144.

[0075] Furthermore, the pipeline connecting the chromatographic column 120 and the adapter assembly 130 passes through the cover 144, so that the chromatographic column 120 can communicate with the first channel 131 of the adapter assembly 130.

[0076] In one embodiment, a socket 113 is further provided in the box body 110. A smart chip is provided in the socket 113, and the socket 113 passes through the box body 110; a plug 212 is provided on the bracket 210. When the chromatographic box 10 is positioned on the bracket 210 by the positioning assembly 230, the plug 212 is plugged into the socket 113. The smart chip is used to identify, read, load, and edit the chromatographic column information integrated in the chromatographic box 10. The chromatographic column information includes but is not limited to the type of packing material, packing density, specification model, production batch, and expiration date.

[0077] It can be understood that in the prior art, the identification method of chromatographic column information is mostly to paste the corresponding bar code on the chromatographic column and then identify it through a bar code scanner. However, there are many problems with the method of identifying chromatographic column information using bar codes, such as limited information storage capacity, strong environmental dependence, low stability, poor material adaptability, and the need to be equipped with a corresponding infrared bar code scanner, resulting in high costs; moreover, there are reading limitations for infrared bar code scanners, and the bar code must be within the line of sight of the infrared bar code scanner to be scanned. In this embodiment, by setting a smart chip, the intelligent automatic identification, reading, and loading of chromatographic column information can be realized by reading the smart chip. In this way, the smart chip in this embodiment not only has a high storage capacity, can be recycled, and has a low cost, but also does not require a special scanning operation and is easy to use. The identification and reading of the smart chip are both prior arts, and the description thereof is omitted in this application.

[0078] Specifically, the plug 212 and the socket 113 are correspondingly arranged. When the chromatographic box 10 reaches the target position, the plug 212 is automatically plugged into the socket 113. In this way, the automation degree of the chromatographic device can be improved, and the replacement process of the chromatographic column 120 can be further simplified.

[0079] In one embodiment, a handle 114 is further provided on the box body 110, which is beneficial to the taking and placing of the chromatographic box 10.

[0080] In one embodiment, participate Figure 6, the positioning component 230 is provided with a positioning groove 231 for receiving the chromatography cartridge 10. A first opening 232 is provided on the side of the positioning groove 231, and the first opening 232 faces the sample injection and sampling unit 220. It can be understood that when the chromatography cartridge 10 is placed in the positioning groove 231, the side wall of the cartridge body 110 through which the transfer assembly 130 is inserted faces the first opening 232, and the transfer assembly 130 is close to the first opening 232. When the sample needle 221 of the sample injection and sampling unit 220 moves from the second position to the first position, the sample needle 221 can pass through the first opening 232 and insert into the first channel 131 of the transfer assembly 130, so that the sample needle 221 is communicated with the chromatographic column 120.

[0081] Further, a second opening 233 is provided at the upper end of the positioning groove 231. In this way, the chromatography cartridge 10 can be conveniently placed into the positioning groove 231 or taken out from the positioning groove 231. Further, the extending direction of the first opening 232 intersects with the extending direction of the second opening 233. In this embodiment, the chromatography cartridge 10 can be placed into the positioning groove 231 or taken out from the positioning groove 231 through the first opening 232, the second opening 233, or the intersection of the first outlet and the second outlet. In this way, the taking and placing of the chromatography cartridge 10 are more convenient.

[0082] In one embodiment, the first opening 232 and the second opening 233 can be the same opening, and the present application does not limit this. As long as the opening can enable the chromatography cartridge 10 to enter the positioning groove 231 and does not affect the insertion of the sample needle 221 into the first channel 131 of the transfer assembly 130. Schematically, the opening is an opening inclined downward towards the sample injection and sampling unit 220. In this way, not only can the sample needle 221 pass through the opening and insert into the first channel 131 of the transfer assembly 130, but also the taking and placing of the chromatography cartridge 10 are convenient.

[0083] In one embodiment, the positioning component 230 includes two relatively arranged positioning plates 234. The positioning groove 231 is defined between the two positioning plates 234. A first plate 235 is provided on the side of the positioning groove 231 opposite to the first opening 232. It can be understood that the first channel 131 in the transfer assembly 130 is sealed, and a certain thrust needs to be applied to the sample needle 221 to insert it into the first channel 131 of the transfer assembly 130. The first plate 235 can provide a abutting site for the chromatography cartridge 10. During the process of the sample needle 221 inserting into the first channel 131, it can prevent the chromatography cartridge 10 from moving along the extending direction of the positioning groove 231 due to the thrust of the sample needle 221 on the transfer assembly 130, so as to ensure that the sample needle 221 smoothly enters the first channel 131 of the transfer assembly 130. Moreover, the positioning groove 231 can provide a limiting effect on the chromatography cartridge 10 to prevent the chromatography cartridge 10 from moving in the positioning groove 231.

[0084] Further, at least part of the channels in the first channel 131 are linear, and at least one end of the sample needle 221 close to the positioning groove 231 is linear. The positioning groove 231 can help the central axis of the linear first channel 131 in the chromatography cartridge 10 to align with the central axis of the linear sample needle 221, thereby further ensuring that the sample needle 221 can smoothly enter the first channel 131 of the transfer assembly 130.

[0085] Specifically, the first plate 235 can be disposed on the bracket 210 or on the side of the positioning plate 234 away from the first opening 232. The present application does not limit this, as long as the first plate 235 and the positioning plate 234 can enclose the positioning groove 231.

[0086] In one embodiment, the length of the positioning groove 231 is longer than the length of the chromatography cartridge 10. In this way, sufficient operating space is provided for the placement and removal of the chromatography cartridge 10 in the positioning groove 231, making the placement and removal of the chromatography cartridge 10 more convenient, and preventing accidental injury to the sample injection and sampling unit 220 when placing and removing the chromatography cartridge 10, especially avoiding accidental injury to the sample needle 221 when placing and removing the chromatography cartridge 10.

[0087] Further, a guiding slide rail 236 is provided at the bottom of the positioning groove 231, and a guiding groove 111 is provided on the chromatography cartridge 10. The guiding groove 111 is slidably engaged with the guiding slide rail 236. In this way, the chromatography cartridge 10 can be pushed along the guiding slide rail 236 to the target position, facilitating the movement of the chromatography cartridge 10 in the positioning groove 231.

[0088] In one embodiment, referring to Figure 5 and Figure 6 , the quick-insert and quick-change mechanism 20 further includes a pressing assembly 240. The pressing assembly 240 includes a pressing member 241, a connecting member 242, and a first elastic member 243. One end of the connecting member 242 is rotatably connected to the bracket 210, the other end of the connecting member 242 is connected to the pressing member 241, and the first elastic member 243 acts on the connecting member 242 or the pressing member 241 to make the pressing member 241 press against the chromatography cartridge 10. By applying pressure to the chromatography cartridge 10 through the pressing assembly 240, in this way, the position of the chromatography cartridge 10 in the positioning groove 231 can be further stabilized, thereby ensuring that the sample needle 221 can smoothly enter the first channel 131 of the transfer assembly 130, and ensuring that the position of the chromatography cartridge 10 does not move when the sample needle 221 leaves the first channel 131.

[0089] The bottom surface of the pressing member 241 is flush with or slightly lower than the top surface of the chromatography cartridge 10. The position where the pressing member 241 is not in contact with the chromatography cartridge 10 is defined as the in-situ position. It can be understood that during the process from when the pressing member 241 just contacts the chromatography cartridge 10 to when the chromatography cartridge 10 moves to the target position, the pressing member 241 will perform an arc-shaped movement with the connection point between the connecting member 242 and the bracket 210 as the center and the distance between the pressing member 241 and this connection point as the radius. In this way, the pressing member 241 will not hinder the smooth arrival of the chromatography cartridge 10 at the target position. As the pressing member 241 gradually moves away from the in-situ position along with the movement of the chromatography cartridge 10, the first elastic member 243 pulls the pressing member 241 back to the in-situ position or applies a pulling force to the pressing member 241 to return it to the in-situ position. In this way, it can be ensured that the pressing member 241 always presses against the chromatography cartridge 10, and the pressure exerted by the pressing assembly 240 on the chromatography cartridge 10 is maximized, and the pressing effect of the pressing assembly 240 on the chromatography cartridge 10 is the best.

[0090] Schematically, the first elastic member 243 is a component such as a spring or a tension belt that can store and release energy and maintain the contact pressure between the connecting member 242 and the chromatography cartridge 10. Specifically, the end of the connecting member 242 away from the pressing member 241 can be rotatably connected to the first plate 235 and can also be rotatably connected to the positioning plate 234. This application does not limit this, as long as the pressing member 241 can press against the chromatography cartridge 10.

[0091] In one embodiment, the pressing member 241 is rotatably connected to the connecting member 242. In this way, after the pressing member 241 contacts the chromatography cartridge 10, the relative movement between the pressing member 241 and the chromatography cartridge 10 is smoother, and the pressing member 241 will not hinder the movement of the chromatography cartridge 10 in the positioning groove 231.

[0092] Specifically, the pressing member 241 is connected to the connecting member 242 through a shaft. The pressing member 241 is threaded through this shaft, and the pressing member 241 can rotate circumferentially around this shaft. Further, the pressing member 241 is a roller or a roll. It can be understood that the above specific embodiments are all illustrative, and this application does not limit this.

[0093] In one embodiment, in the height direction of the chromatography cartridge 10, a limiting member 112 protrudes from the top surface of the chromatography cartridge 10. When one end of the chromatography cartridge 10 abuts against the first plate 235, the pressing member 241 presses against the limiting member 112. The limiting member 112 can limit the pressing member 241. In this way, the relative movement between the pressing member 241 and the chromatography cartridge 10 can be avoided, and the pressing effect of the pressing member 241 on the chromatography cartridge 10 can be improved.

[0094] Specifically, the limiting member 112 is wavy. The limiting member 112 includes two wave crests and a wave trough clamped between the two wave crests. When one end of the chromatography cartridge 10 abuts against the first plate 235, the pressing member 241 presses against the wave trough, and the two wave crests can limit the pressing member 241. In this way, the pressing effect of the pressing member 241 on the chromatography cartridge 10 can be further improved, and the relative movement between the pressing member 241 and the chromatography cartridge 10 can be avoided. When the wave crest close to the pressing member 241 reaches the position of the pressing member 241, the pressing member 241 moves along the surface of the wave crest and is lifted by the wave crest to the highest point of the wave crest. Subsequently, the pressing member 241 gradually descends with the alternation of the wave crest and the wave trough and the pressure of the first elastic member 243, and finally presses against the position of the wave trough.

[0095] In one embodiment, the pressing assembly 240 further includes a shock pad 244. In the height direction of the positioning plate 234, the shock pad 244 is disposed between the upper end of the positioning plate 234 and the connecting member 242, and the shock pad 244 is connected to the positioning plate 234 and abuts against the connecting member 242. It can be understood that when the chromatography cartridge 10 is separated from the pressing member 241, the pressing member 241 will move towards the positioning groove 231 under the action of the pressure of the first elastic member 243 and gravity, and the shock pad 244 can prevent the pressing member 241 from touching the positioning plate 234, thereby ensuring the stability of the positioning plate 234 and the positioning groove 231; and the shock pad 244 can also keep the pressing member 241 in place.

[0096] In one embodiment, the upper part of the positioning plate 234 is provided with a first flanging 2341 extending in a direction away from the positioning groove 231, and the shock pad 244 is connected to the first flanging 2341. The first flanging 2341 can increase the connection area between the positioning plate 234 and the shock pad 244. In this way, the connection of the shock pad 244 on the first flanging 2341 is more stable, and further the shock pad 244 can better play a shock-absorbing role. Moreover, when the chromatography cartridge 10 is placed in the positioning groove 231, the first flanging 2341 can prevent the edge of the positioning plate 234 from cutting the chromatography cartridge 10.

[0097] Further, a second flanging 2342 is provided on the side of the positioning plate 234 away from the sample inlet / outlet unit 220, and the second flanging 2342 extends in a direction away from the positioning groove 231. The second flanging 2342 is connected to the edge of the first plate 235. That is to say, in the width direction of the positioning groove 231, there is a certain space between the side of the positioning plate 234 away from the positioning groove 231 and the edge of the first plate 235. The first elastic member 243 can be placed in this space. Thus, this space can provide protection for the first elastic member 243 to prevent the first elastic member 243 from being accidentally touched and falling off, thereby ensuring the stability of the first elastic member 243 applying a pulling force to the pressing member 241, ensuring the pressing effect of the pressing assembly 240 on the chromatography cartridge 10, and making the quick insertion and replacement mechanism 20 more compact.

[0098] Further, a third flanging 2343 is provided at the first opening 232 of the positioning plate 234, and the third flanging 2343 extends in a direction away from the positioning groove 231. The third flanging 2343 can provide a abutting position for the sample inlet / outlet unit 220.

[0099] In one embodiment, a first position detector 211 is further provided on the bracket 210. The first position detector 211 is used to detect the position of the chromatography cartridge 10, and the first position detector 211 is communicatively connected to the sample inlet / outlet unit 220. After the first position detector 211 detects that the chromatography cartridge 10 reaches the target position, the sample inlet / outlet unit 220 can drive the sample needle 221 to move to the first position. Thus, the sample needle 221 can be automatically moved between the first position and the second position, thereby improving the automation degree of the chromatography device and further simplifying the replacement process of the chromatography column 120.

[0100] In one embodiment, the sample inlet / outlet unit 220 further includes a driving assembly 223 and a pushing assembly 222. Both sample needles 221 are provided on the pushing assembly 222. The driving assembly 223 drives the pushing assembly 222 to move, so as to drive the two sample needles 221 to move between the first position and the second position. By controlling the movement of the sample needles 221 between the first position and the second position through the driving assembly 223 and the pushing assembly 222, the automation degree of the chromatography device is improved, and the replacement process of the chromatography column 120 is further simplified.

[0101] In one embodiment, the propulsion assembly 222 includes a fixed plate 2221, a clamping plate 2222, a second elastic member 2223 and a guide shaft 2224. The fixed plate 2221 and the clamping plate 2222 are arranged opposite to each other, one end of the sample needle 221 is fixed to the fixed plate 2221, and the other end of the sample needle 221 is inserted through the clamping plate 2222. The guide shaft 2224 is movably inserted through the fixed plate 2221, one end of the guide shaft 2224 is fixedly connected to the clamping plate 2222, and the other end of the guide shaft 2224 is provided with a stopper 2225, which stops at one side of the fixed plate 2221 to limit the guide shaft 2224 from being separated from the fixed plate 2221. The fixed plate 2221 can move along the axial direction of the guide shaft 2224 under the drive of the driving member 2231, and drive the sample needle 221 to move between the first position and the second position. The second elastic member 2223 is sleeved on the guide shaft 2224, and the two ends of the second elastic member 2223 are respectively pressed against the fixed plate 2221 and the pressing plate 2222, so that the stopper 2225 is pressed against the side of the fixed plate 2221 away from the pressing plate 2222. In this way, the linear motion of the sample needle 221 can be ensured, making it easier for the sample needle 221 to be inserted into the first channel 131 of the adapter assembly 130. In addition, within a certain position range, the second elastic member 2223 can make the pressing plate 2222 move synchronously with the fixed plate 2221, so that the pressing plate 2222 and the fixed plate 2221 remain relatively still and stable, so as to further stabilize the motion of the sample needle 221.

[0102] It is understandable that the axial direction of the guide shaft 2224 is the same as the direction in which the sample needle 221 moves between the first position and the second position. Driven by the driving assembly 223, the fixed plate 2221 can move along the axial direction of the guide shaft 2224, that is, move in a direction close to or away from the adapter assembly 130. Since the second elastic member 2223 is sleeved on the guide shaft 2224, and the two ends of the second elastic member 2223 are respectively abutted against the fixed plate 2221 and the clamping plate 2222, when the fixed plate 2221 moves in a direction close to the adapter assembly 130, it can also drive the clamping plate 2222 to move in a direction close to the adapter assembly 130, and can also drive the sample needle 221, the second elastic member 2223 and the guide shaft 2224 to move in a direction close to the adapter assembly 130. From the above, it can be seen that the adapter assembly 130 is arranged in the positioning groove 231 surrounded by the positioning plate 234, and the side of the positioning groove 231 is provided with a first opening 232 facing the clamping plate 2222. After the clamping plate 2222 abuts against the first opening 232, the clamping plate 2222 and the guide shaft 2224 cannot continue to move toward the adapter assembly 130, while the fixed plate 2221 continues to move toward the direction close to the adapter assembly 130. At this time, the fixing plate 2221 is separated from the stopper 2225, and the fixing plate 2221 begins to squeeze the second elastic member 2223, causing the second elastic member 2223 to enter a compressed state from the original state, and the end of the sample needle 221 close to the adapter assembly 130 begins to move relative to the clamping plate 2222, and the end of the sample needle 221 gradually moves away from the clamping plate 2222 and gradually approaches the adapter assembly 130 until it is inserted into the first channel 131 of the adapter assembly 130, and the fixing plate 2221 stops moving toward the direction close to the adapter assembly 130.

[0103] When the fixing plate 2221 moves from the above position toward the direction away from the adapter assembly 130 driven by the driving assembly 223, there is a relative movement between the end of the sample needle 221 close to the adapter assembly 130 and the pressing plate 2222, and the end of the sample needle 221 is separated from the first channel 131 of the adapter assembly 130, and the end gradually moves away from the adapter assembly 130 and gradually approaches the pressing plate 2222, until the second elastic member 2223 gradually recovers from the compressed state to the original state, and the fixing plate 2221 abuts against the stopper 2225 of the guide shaft 2224. Then, the fixing plate 2221 continues to move toward the direction away from the adapter assembly 130 driven by the driving assembly 223, and at this time, the fixing plate 2221 can simultaneously drive the sample needle 221, the pressing plate 2222, the second elastic member 2223 and the guide shaft 2224 to move toward the direction away from the adapter assembly 130.

[0104] Furthermore, a third flange 2343 is provided at the first opening 232 of the positioning plate 234, that is, a third flange 2343 is provided at the end of the positioning plate 234 close to the pressing plate 2222. The pressing plate 2222 can abut against the third flange 2343. The third flange 2343 increases the contact area between the pressing plate 2222 and the first opening 232 of the positioning plate 234, so that a better abutment position can be provided for the pressing plate 2222, and the edge of the positioning plate 234 can be prevented from cutting the pressing plate 2222.

[0105] In one embodiment, a groove 2226 is provided on one side of the pressing plate 2222 close to the adapter assembly 130; the adapter assembly 130 can be accommodated in the groove 2226. The outer wall of the adapter assembly 130 abuts against a portion of the outer circumference of the sealing mounting cover 134 of the adapter assembly 130, so that the central axis of the sample needle 221 and the central axis of the first channel 131 of the adapter assembly 130 are coaxial, so that the sample needle 221 can accurately enter the first channel 131.

[0106] In one embodiment, the driving assembly 223 includes a driving member 2231, a screw rod 2232, a slider 2233 and a first guide rail 2234. The slider 2233 is slidably matched with the first guide rail 2234. The fixing plate 2221 is connected to the slider 2233. The driving member 2231 is used to drive the screw rod 2232 to rotate, so that the slider 2233 moves along the axial direction of the screw rod 2232, and the fixing plate 2221 drives the sample needle 221 to move between the first position and the second position. In this way, the driving member 2231 can drive the sample needle 221 to move between the first position and the second position.

[0107] It can be understood that the axial direction of the screw rod 2232 is the same as the axial direction of the guide shaft 2224. Specifically, the driving member 2231 is a motor, and the central axis of the output shaft of the motor is coaxial with the central axis of the screw rod 2232. In this way, the motor can be directly connected to the screw rod 2232 through its output shaft, without using a transmission member to transmit the driving force of the motor to the screw rod 2232, thereby saving costs.

[0108] In other embodiments, the driving member 2231 is a motor, the central axis of the output shaft of the motor is not coaxial with the central axis of the screw rod 2232, and the driving assembly 223 further includes a transmission group 2235, the transmission member connects the motor and the screw rod 2232, and the motor transmits its own driving force to the screw rod 2232 through the transmission member. In this way, the position of the motor can be set arbitrarily, thereby improving the flexibility and universality of the chromatographic device.

[0109] In one embodiment, the sample inlet and outlet unit 220 further includes a mounting base 224, and the first guide rail 2234 is disposed on the mounting base 224. In this way, the support for the first guide rail 2234 is increased; and by adjusting the height of the mounting base 224, the central axis of the sample needle 221 can be coaxial with the central axis of the first channel 131 of the transfer assembly 130.

[0110] In one embodiment, the sample inlet and outlet unit 220 further includes a second position detector 225 for detecting the position of the fixed plate 2221. The second position detector 225 is communicatively connected to both the PLC and the driving member 2231. Based on the position of the fixed plate 2221 detected by the second position detector 225, the PLC controls the driving member 2231 to start driving the fixed plate 2221 to move or stop the fixed plate 2221 from moving. Further, the second position detector 225 is a photoelectric switch and a baffle used in cooperation with the photoelectric switch. The baffle is disposed on a side of the fixed plate 2221 away from the pressing plate 2222, and the photoelectric switch is disposed on the support 210.

[0111] In one embodiment, the end of the sample needle 221 close to the transfer assembly 130 is in a contracted shape. Define the end of the sample needle 221 close to the transfer assembly 130 as the first site, and the position adjacent to the end as the second site. The sample needle 221 gradually narrows from the second site towards the first site. In this way, it is more convenient to insert the sample needle 221 into the first channel 131 of the transfer assembly 130.

[0112] The present application also provides a chromatography system, including the chromatography device described in any of the above embodiments.

[0113] In the prior art, multiple chromatographic columns are placed in the same column incubator, and the column incubator needs to be equipped with a large heating element, making the incubator and the chromatography system relatively large in volume and high in cost. Moreover, when it is necessary to replace one of the chromatographic columns in the column incubator, since it is necessary to open the door or lid of the column incubator and it takes a certain amount of time to replace one chromatographic column, this will cause temperature fluctuations in the entire column incubator, thereby affecting the temperature control of other normally operating chromatographic columns and the stability and reliability of the detection results.

[0114] In this embodiment, multiple chromatography devices can be arranged simultaneously, and each chromatography device only includes 1 chromatographic column 120. In this way, each chromatographic column 120 can be temperature-controlled independently, and since the chromatographic columns 120 are independent of each other, the temperature control of each chromatographic column 120 is more accurate, avoiding temperature interference between multiple chromatographic columns 120, and making the detection results of the chromatography device for samples more stable and reliable.

[0115] The present application also provides a method for replacing a chromatographic column. The replacement method is used to replace the chromatographic column in the chromatographic device in any of the above embodiments. The chromatographic cassette includes a first chromatographic cassette and a second chromatographic cassette. The first chromatographic cassette is the chromatographic cassette located on the bracket of the chromatographic device before replacing the chromatographic column, and the second chromatographic cassette is the chromatographic cassette located on the bracket of the chromatographic device after replacing the chromatographic column.

[0116] The replacement method includes the following steps:

[0117] The sample needle moves from the first position to the second position, and the sample needle is separated from the transfer assembly of the first chromatographic cassette;

[0118] Take out the first chromatographic cassette from the bracket;

[0119] Position the second chromatographic cassette on the bracket;

[0120] The sample needle moves from the second position to the first position, the sample needle is inserted into the first channel of the transfer assembly of the second chromatographic cassette, and the sample needle is in communication with the first channel of the second chromatographic cassette, thereby completing the replacement of the chromatographic column.

[0121] It can be understood that before installing the second chromatographic cassette into the chromatographic device, a new chromatographic column needs to be installed in the second chromatographic cassette in advance, and the new chromatographic column is connected to the transfer assembly. Since when preparing the second chromatographic cassette, it is not necessary to pause the operation of the chromatographic device, it can be prepared before removing the first chromatographic cassette. For the chromatographic device, when the sample needle moves to the second position, after the sample needle and the transfer assembly are separated, directly take out the first chromatographic cassette, then place the second chromatographic cassette at the target position, and when the sample needle moves to the first position, make the inside of the sample needle communicate with the first channel. In this way, the rapid replacement of the chromatographic cassette and the chromatographic column is achieved. By integrating the chromatographic column into the chromatographic cassette and replacing the chromatographic cassette to quickly replace the chromatographic column, the present application can simplify the process of replacing the chromatographic column and save the time for replacing the chromatographic column.

[0122] The technical features of the above embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0123] The above embodiments only represent several implementation manners of the present application, and the description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the patent protection scope of the present application should be subject to the appended claims.

Claims

1. A chromatographic device, characterized in that It comprises a chromatographic box (10) and a quick-insert and quick-change mechanism (20). The chromatographic box (10) comprises a box body (110) and a chromatographic column (120) disposed in the box body (110), both ends of the chromatographic column (120) being connected to a transfer assembly (130) via a pipeline, the two transfer assemblies (130) being disposed through a side wall of the box body (110), the transfer assembly (130) being provided with a first channel (131), the first channel (131) being in communication with the interior of the chromatographic column (120), The quick-insert and quick-change mechanism (20) comprises a bracket (210) and a sample inlet and outlet unit (220) disposed on the bracket (210); the bracket (210) is provided with a positioning component (230); the positioning component (230) is used to position the chromatographic box (10); The sample inlet and outlet unit (220) comprises two movable sample needles (221), and the two sample needles (221) are used to be connected to two adapter components (130) respectively. When the chromatographic box (10) is positioned on the bracket (210) by the positioning component (230), the sample needle (221) can move between a first position and a second position. When the sample needle (221) moves to the first position, the sample needle (221) is inserted into the first channel (131) of the adapter component (130) so that the interior of the sample needle (221) and the first channel (131) are connected. When the sample needle (221) moves to the second position, the sample needle (221) and the adapter component (130) are separated, so that the chromatographic column (120) in the chromatographic device can be replaced by replacing the chromatographic box (10).

2. The chromatographic device according to claim 1, characterized in that The quick-insert and quick-change mechanism (20) further comprises a clamping assembly (240), wherein the clamping assembly (240) comprises a clamping member (241), a connecting member (242) and a first elastic member (243). One end of the connecting member (242) is rotatably connected to the bracket (210), and the other end of the connecting member (242) is connected to the pressing member (241). The first elastic member (243) acts on the connecting member (242) or the pressing member (241) to press the pressing member (241) against the chromatography box (10).

3. The chromatographic device according to claim 1, characterized in that Two ends of the adapter component (130) are respectively arranged on two sides of the side wall of the box body (110); The adapter assembly (130) comprises a sealing member (132), a sealing mounting seat (133) and a sealing mounting cover (134); the sealing mounting seat (133) is disposed through a side wall of the box body (110); the sealing mounting seat (133) and the sealing mounting cover (134) are detachably connected; the sealing mounting seat (133) and the sealing mounting cover (134) enclose a sealed space; the sealing member (132) is disposed in the sealed space; and the sealing member (132) is provided with the first channel (131); When the sample needle (221) is in the first position, the sample needle (221) is inserted into the sealing installation cover (134), and the outer wall of the sample needle (221) is tightly fitted with the first channel (131).

4. The chromatographic device according to claim 3, characterized in that The adapter assembly (130) further comprises a sealing pressure ring (135), wherein the sealing pressure ring (135) is arranged between the sealing member (132) and the sealing installation cover (134) so ​​as to press the sealing member (132) against the sealing installation seat (133).

5. The chromatographic device according to claim 1, characterized in that The chromatography box (10) is provided with a heating sub-box (140), and the heating sub-box (140) is provided with a tightly fitting pressing plate (142) and a heating component (143), a fixing groove (1431) is provided between the pressing plate (142) and the heating component (143), and the fixing groove (1431) is used to accommodate at least a part of the chromatography column (120), and the heating component (143) is used to heat the chromatography column (120) accommodated in the fixing groove (1431).

6. The chromatographic device according to claim 5, characterized in that The pipeline comprises an injection pipeline (121), one end of the injection pipeline (121) is connected to a first channel (131) of one of the adapter components (130), and the other end of the injection pipeline (121) is connected to an injection port of the chromatographic column (120); the fixed groove (1431) is also used to accommodate part of the injection pipeline (121); and the heating component (143) is also used to heat the part of the injection pipeline (121) accommodated in the fixed groove (1431).

7. The chromatographic device according to claim 5, characterized in that The heating assembly (143) comprises a heating element (1432), a heat conducting element (1433), a temperature sensor (1434) and a temperature protection switch (1435); one side of the heat conducting element (1433) is connected to the heating element (1432); the other side of the heat conducting element (1433) is tightly fitted to the pressing plate (142); the fixing groove (1431) is arranged between the pressing plate (142) and the heat conducting element (1433); The heating element (1432) is used to heat the chromatographic column (120) accommodated in the fixing groove (1431); The temperature sensor (1434) is used to detect the temperature of the heat conducting member (1433); The temperature sensor (1434) and the heating element (1432) are both communicatively connected to the temperature protection switch (1435), and the temperature protection switch (1435) is used to turn on or off the heating element (1432) according to the temperature detected by the temperature sensor (1434).

8. The chromatographic device according to claim 1, characterized in that A socket (113) is also provided in the box body (110), a smart chip is provided in the socket (113), and the socket (113) is inserted through the box body (110); The support (210) is provided with a plug-in component (212), and when the chromatography box (10) is positioned on the support (210) by the positioning component (230), the plug-in component (212) is plugged into the socket (113).

9. A chromatography system, characterized in that: Comprising a chromatography device as claimed in any one of claims 1 to claim 8.

10. A method for replacing a chromatographic column, characterized in that: The replacement method is used to replace a chromatographic column in a chromatographic device according to any one of claims 1 to 8, wherein the chromatographic box comprises a first chromatographic box and a second chromatographic box, wherein the first chromatographic box is a chromatographic box positioned on the support of the chromatographic device before the chromatographic column is replaced, and the second chromatographic box is a chromatographic box positioned on the support of the chromatographic device after the chromatographic column is replaced. The replacement method includes the following contents: The sample needle moves from the first position to the second position, and the sample needle is separated from the adapter component of the first chromatographic box; taking the first chromatography box out of the support; positioning the second chromatography box on the support; The sample needle moves from the second position to the first position, the sample needle is inserted into the first channel of the adapter assembly of the second chromatographic box, and the sample needle is communicated with the first channel of the second chromatographic box, thereby completing the replacement of the chromatographic column.

Images