Self-adaptive photoacoustic microscopic imaging method, device and equipment

Abstract

The invention relates to the technical field of photoacoustic microscopic imaging, and discloses a self-adaptive photoacoustic microscopic imaging method, device and equipment. The method is applied to a self-adaptive photoacoustic microscopic imaging device. The device comprises an initial photoacoustic microscopic imaging assembly, a continuous wave laser and a spatial light modulator. The method comprises the following steps: controlling an initial photoacoustic microscopic imaging assembly to emit first pulse laser and second pulse laser, and controlling a continuous wave laser to emit continuous wave laser; controlling a spatial light modulator to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser; wherein when the spatial light modulator carries out wavefront modulation, the differential spectroacoustic signal is used as feedback. According to the invention, the greedy algorithm is used to optimize the phase diagram displayed on the SLM, the aberration caused by the absorber is dynamically compensated point by point through the GR effect, the microstructure which cannot be distinguished by the traditional PAM is disclosed, and the signal intensity and the lateral resolution can be effectively improved by compensating the aberration.

Description

Technical Field

[0001] The present application relates to the field of photoacoustic microscopy technology, and in particular to an adaptive photoacoustic microscopy method, device and equipment. Background Art

[0002] Photoacoustic microscopy (PAM) uses non-ionizing photons and low-scattering ultrasound to image absorbers. All absorbers have the property of absorbing light, so PAM has been widely used. PAM combines the principles of optical excitation and acoustic detection: when a pulsed focused beam excites the absorber, the absorber generates ultrasound due to the absorption of heat, which can achieve lateral resolution as low as the optical diffraction limit of the submicron level. This mode is called optical-resolution photoacoustic microscopy (OR-PAM). However, absorbers are inherently inhomogeneous, which distorts the light wavefront and induces optical aberrations during light propagation. Therefore, the imaging quality decreases with the increase of imaging depth. Summary of the invention

[0003] In view of this, the present application provides an adaptive photoacoustic microscopy method, device and equipment to solve the problem that optical aberrations are caused during light propagation during photoacoustic microscopy imaging, thereby causing the imaging quality to decrease with the increase of imaging depth.

[0004] In a first aspect, the present application provides an adaptive photoacoustic microscopy method, which is applied to an adaptive photoacoustic microscopy device, the device comprising: an initial photoacoustic microscopy component, a continuous wave laser and a spatial light modulator; the method comprises: controlling the initial photoacoustic microscopy component to emit a first pulse laser and a second pulse laser, and controlling the continuous wave laser to emit a continuous wave laser; controlling the spatial light modulator to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser; wherein, when performing wavefront modulation, the spatial light modulator uses a differential photoacoustic signal as feedback.

[0005] In a second aspect, the present application provides an adaptive photoacoustic microscopy imaging device, comprising: an initial photoacoustic microscopy imaging component, a continuous wave laser and a spatial light modulator; wherein the initial photoacoustic microscopy imaging component is used to acquire an image of a target to be imaged based on a first pulse laser and a second pulse laser; the continuous wave laser is used to emit a continuous wave laser to the target to be imaged during the imaging process of the initial photoacoustic microscopy imaging component to produce a GR effect; the spatial light modulator is used to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser; wherein the spatial light modulator uses a differential photoacoustic signal as feedback when performing wavefront modulation.

[0006] In an optional implementation, the differential photoacoustic signal is obtained according to the following steps:

[0007] Obtaining the photoacoustic amplitudes of the first pulse laser and the second pulse laser under preset conditions; wherein the preset conditions are: the light flux obeys a two-dimensional Gaussian distribution in the lateral direction;

[0008] A differential photoacoustic signal between the first pulse laser and the second pulse laser is obtained according to the photoacoustic amplitudes of the first pulse laser and the second pulse laser under preset conditions.

[0009] In an optional embodiment, the initial photoacoustic microscopy imaging assembly includes: a laser transmitter, a laser focusing tool, an ultrasonic transducer and a data processing device.

[0010] In an optional implementation, the spatial light modulator is used to perform the following steps to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser:

[0011] Obtaining a plurality of ordered Zernike polynomials; wherein the Zernike polynomials are used to describe the optical aberrations generated by the initial photoacoustic microscopy imaging component;

[0012] Loading a plurality of ordered Zernike polynomials into a spatial light modulator in sequence, and determining coefficients corresponding to the plurality of ordered Zernike polynomials; wherein, in the process of determining the coefficients corresponding to the plurality of ordered Zernike polynomials, the differential photoacoustic signal becomes larger;

[0013] The first pulse laser, the second pulse laser and the continuous wave laser are subjected to wavefront modulation according to coefficients corresponding to a plurality of ordered Zernike polynomials.

[0014] In an optional implementation, a plurality of ordered Zernike polynomials are sequentially loaded into a spatial light modulator, and coefficients corresponding to the plurality of ordered Zernike polynomials are determined, including:

[0015] Establishing a feedback loop between the spatial light modulator and the ultrasound transducer and data processing equipment in the initial photoacoustic microscopy imaging assembly;

[0016] According to the feedback loop, a plurality of ordered Zernike polynomials are traversed to determine coefficients corresponding to the plurality of ordered Zernike polynomials.

[0017] In an optional implementation, the spatial light modulator is a reflective liquid crystal spatial light modulator.

[0018] In an optional implementation, the center frequency of the ultrasonic transducer is 50 MHz and the bandwidth is 31.40-73.25 MHz.

[0019] In an optional implementation, the order of the Zernike polynomial is 4-10.

[0020] In a third aspect, the present application provides a computer device, comprising: a memory and a processor, the memory and the processor being communicatively connected to each other, the memory storing computer instructions, and the processor executing the above-mentioned first aspect or any corresponding embodiment thereof applied to an adaptive photoacoustic microscopy imaging method by executing the computer instructions.

[0021] The beneficial effects of this application are:

[0022] Compared with other 3D optical microscopy techniques combined with AO assistance, the adaptive photoacoustic microscopy method provided in this application takes into account defocus correction by using an adaptive photoacoustic microscopy device (AWA-GR-PAM), and uses an intensity-modulated continuous-wave (CW) laser to heat the absorber to produce the GR effect, which can achieve tighter focusing of the light beam. The acoustic differential signal is then used instead of the optical signal to indirectly determine the optimized wavefront. This application uses a greedy algorithm to optimize the phase map displayed on the spatial light modulator SLM, and dynamically compensates for the aberrations caused by the absorber point by point through the GR effect, revealing microstructures that cannot be distinguished by traditional PAM. Compensating for aberrations can effectively improve signal intensity and lateral resolution. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the specific implementation methods of the present application or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0024] Figure 1is a flow chart of a photoacoustic microscopy method provided according to one embodiment of the present application;

[0025] Figure 2 Schematic diagram of the working principle of a photoacoustic microscopy imaging device (AWA-GR-PAM) provided according to one embodiment of the present application;

[0026] Figure 3 is a schematic structural diagram of a photoacoustic microscopy imaging device (AWA-GR-PAM) provided according to an embodiment of the present application;

[0027] Figure 4 is a timing diagram of a laser emitter, a continuous wave laser, and a photoacoustic signal in an initial photoacoustic microscopy imaging assembly provided according to one embodiment of the present application;

[0028] Figure 5 is a 2D MAP image of carbon fiber filaments obtained through AWA-GR correction according to an embodiment of the present application;

[0029] Figure 6 is a 2D MAP image of a carbon fiber filament obtained without AWA-GR correction according to an embodiment of the present application;

[0030] Figure 7 It is a normalized strength distribution diagram of carbon fiber filaments obtained after AWA-GR correction and without AWA-GR correction according to one embodiment of the present application;

[0031] Figure 8 yes Figure 3 Phase diagram at the solid line frame position;

[0032] Fig. 9 It is a schematic diagram of the hardware structure of the computer device of an embodiment of the present application. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of this application.

[0034] Photoacoustic microscopy (PAM) uses non-ionizing photons and low-scattering ultrasound to image absorbers. All absorbers have the property of absorbing light, so PAM has been widely used. PAM combines the principles of optical excitation and acoustic detection: when a pulsed focused beam excites the absorber, the absorber generates ultrasound due to the absorption of heat, which can achieve lateral resolution as low as the optical diffraction limit of the submicron level. This mode is called optical-resolution photoacoustic microscopy (OR-PAM). However, absorbers are inherently inhomogeneous, which distorts the light wavefront and induces optical aberrations during light propagation. Therefore, the imaging quality decreases with the increase of imaging depth.

[0035] Therefore, the present application provides an adaptive photoacoustic microscopy method, device and apparatus, such as Figure 1 As shown, a photoacoustic microscopy imaging method is provided in the present application, and the details are as follows.

[0036] Step S1, controlling the initial photoacoustic microscopy imaging component to emit a first pulse laser and a second pulse laser, and controlling the continuous wave laser to emit a continuous wave laser.

[0037] Step S2, controlling the spatial light modulator to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser; wherein the spatial light modulator uses the differential photoacoustic signal as feedback when performing wavefront modulation.

[0038] The above-mentioned adaptive photoacoustic microscopy method is applied to an adaptive photoacoustic microscopy device, which includes: an initial photoacoustic microscopy component, a continuous wave laser and a spatial light modulator. Figure 2-4 As shown, an adaptive photoacoustic microscopy imaging device provided by the present application is shown. The above device includes: an initial photoacoustic microscopy imaging component, a continuous wave laser and a spatial light modulator;

[0039] The adaptive photoacoustic microscopy imaging device provided in the present application is an acoustic feedback wavefront adaptive photoacoustic microscopy imaging device (AWA-GR-PAM) combined with Gruneisen relaxation, which includes an initial photoacoustic microscopy imaging component, a continuous wave laser and a spatial light modulator.

[0040] The initial photoacoustic microscopy imaging assembly is used to acquire an image of the target to be imaged according to the first pulse laser and the second pulse laser.

[0041] Specifically, the initial photoacoustic microscopy imaging assembly includes: a laser transmitter, a laser focusing tool, an ultrasonic transducer and a data processing device.

[0042] The bandwidth of the ultrasonic transducer is 31.40-73.25 MHz, and the center frequency is 50 MHz.

[0043] The above-mentioned continuous wave laser is used to emit continuous wave laser to the target to be imaged during the imaging process of the initial photoacoustic microscopy imaging component to produce the GR effect.

[0044] Specifically, an intensity-modulated continuous-wave (CW) laser is used to heat the absorber to produce the GR effect, which enables tighter focusing of the light beam.

[0045] Among them, the GR effect, or Gruneisen relaxation, is a nonlinear phenomenon in the photoacoustic effect. In traditional photoacoustic imaging, there is a linear relationship between the signal amplitude and the absorption coefficient. However, in the GR effect, this relationship becomes a quadratic nonlinearity, that is, the signal amplitude is proportional to the square of the absorption coefficient. This nonlinear relationship provides new perspectives and applications for photoacoustic imaging.

[0046] The GR effect in this application describes the change of the Gruneissen parameter caused by the heating of the absorber by the first pulse laser and the second pulse laser.

[0047] The above-mentioned spatial light modulator is used to perform wavefront modulation on the above-mentioned first pulse laser, the above-mentioned second pulse laser and the above-mentioned continuous wave laser; wherein, when performing wavefront modulation, the above-mentioned spatial light modulator uses the differential photoacoustic signal as feedback.

[0048] The differential photoacoustic signal is obtained according to the following steps:

[0049] Step a1, obtaining the photoacoustic amplitude of the first pulse laser and the second pulse laser under preset conditions; wherein the preset conditions are: the light flux in the lateral direction obeys a two-dimensional Gaussian distribution;

[0050] Step a2, obtaining a differential photoacoustic signal between the first pulse laser and the second pulse laser according to the photoacoustic amplitudes of the first pulse laser and the second pulse laser under preset conditions.

[0051] In this embodiment, as the temperature increases, the Gruneisen parameter increases, thereby generating a stronger photoacoustic signal. Specifically, two identical nanosecond laser pulses (a first pulse laser and a second pulse laser) sequentially excite the absorber (the target to be imaged) with a sub-microsecond delay, and the initial voltage boost generated by the first pulse laser can be expressed as:

[0052] p 1 =Γ 0 η th μ a F (1)

[0053] Among them, Γ0 is the Gruneiss parameter at the initial temperature, η th is the thermal conversion efficiency, μ a is the light absorption coefficient, and F is the light flux. During the thermal relaxation time of the first laser pulse, the second laser pulse excites the same absorber, resulting in another initial boost:

[0054] p 2 =(Γ 0 +Gη th μ a F)η th μ a F (2)

[0055] where G is the coefficient that relates the change in the Gruneissen parameter to the energy absorbed by the first laser pulse. Assuming that the light flux follows a two-dimensional Gaussian distribution in the transverse direction, the photoacoustic amplitude generated by the first laser pulse can be expressed as:

[0056]

[0057] Where k is a constant related to the sensitivity of photoacoustic signal detection, E is the pulse energy, w is the waist of the Gaussian beam, and x and y are the transverse coordinates. Similarly, the photoacoustic amplitude generated by the second laser pulse can be expressed as:

[0058]

[0059] Perform differential operation on equations (3) and (4) to obtain the differential photoacoustic signal:

[0060]

[0061] Comparing equation (3) and equation (5), it can be seen that the resolution of imaging using the GR effect is √2 higher than that of traditional PAM, which can achieve tighter focusing of the light beam. The present application uses an intensity modulated CW laser to heat the absorber, which is convenient for cost saving.

[0062] Furthermore, the spatial light modulator is used to perform the following steps to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser:

[0063] Step b1, obtaining a plurality of ordered Zernike polynomials; wherein the above Zernike polynomials are used to describe the optical aberrations produced by the initial photoacoustic microscopy imaging component.

[0064] Step b2, loading a plurality of ordered Zernike polynomials into the spatial light modulator in sequence, and determining coefficients corresponding to the plurality of ordered Zernike polynomials; wherein, in the process of determining the coefficients corresponding to the plurality of ordered Zernike polynomials, the differential photoacoustic signal becomes larger.

[0065] Here, a series of ordered Zernike polynomials are sequentially loaded into the spatial light modulator SLM. These Zernike polynomials are orthogonal to each other within the circular pupil, and each polynomial represents an optical aberration.

[0066] Step b3, performing wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser according to coefficients corresponding to a plurality of ordered Zernike polynomials.

[0067] Among them, a plurality of ordered Zernike polynomials are sequentially loaded into the spatial light modulator, and coefficients corresponding to the plurality of ordered Zernike polynomials are determined, including:

[0068] First, a feedback loop is constructed between the spatial light modulator and the ultrasonic transducer and the data processing device in the initial photoacoustic microscopy imaging assembly. Then, according to the feedback loop, a plurality of ordered Zernike polynomials are traversed to determine the coefficients corresponding to the plurality of ordered Zernike polynomials.

[0069] By building a feedback loop between the spatial light modulator and the ultrasonic transducer and data processing equipment in the initial photoacoustic microscopy imaging assembly, the coefficients of each order of Zernike polynomials are traversed and determined. This feedback loop ensures that the differential photoacoustic signal ΔPA(x,y) is continuously enhanced after correcting each order of aberration. Since ΔPA(x,y) does not include the unlabeled photoacoustic signal, it is related to E 2 The AWA-GR-PAM can effectively compensate for the optical aberrations of both large and small features. The AWA-GR-PAM does not require optical wavefront sensing to determine the optical aberrations, and is particularly suitable for PAM for ultrasonic testing. At the same time, Zernike polynomials can well represent optical aberrations, so the feedback process is very effective, and in this application, only low-order Zernike polynomials are needed to solve the optical aberration problem.

[0070] It should be noted that the working principle of AWA-GR-PAM provided in this application is as follows Figure 2 As shown: The initial photoacoustic microscopy imaging component emits a first pulse laser to generate a reference photoacoustic signal; after being heated by the continuous wave laser emitted by the continuous wave laser, the second pulse laser is triggered to generate a photoacoustic signal with a larger amplitude, and the spatial light modulator SLM modulates the wavefronts of the first pulse laser, the second pulse laser, and the continuous wave laser to compensate for the aberration caused by the absorber. The phase image displayed by the spatial light modulator SLM is the inversion of the distorted wavefront, thereby eliminating distortion and producing a high-quality focus at depth. In order to obtain the optimal phase image, a feedback loop is established between the spatial light modulator SLM, the ultrasonic transducer, and the computer to optimize the differential photoacoustic signal amplitude, where ΔPA N>…>ΔPA 2 >ΔPA 1 .

[0071] According to the following Figure 3-Figure 4 The photoacoustic microscopy imaging device provided in the present application is described with reference to the corresponding specific device embodiments.

[0072] Among them, Figure 3 As shown, the photoacoustic microscopy imaging device includes a 532nm nanosecond pulse laser (laser transmitter in the initial photoacoustic microscopy imaging component) and a 532nm CW laser (continuous wave laser), wherein the pulse intensity of the pulse laser fluctuates greatly, which will affect the feedback signal. In this embodiment, a photodiode is used to monitor the intensity of the light reflected by the beam splitter, and the measured value is used for normalization. Then, the combination of the half-wave plate and the polarization beam splitter sets the combined light (the first pulse laser, the second pulse laser and the continuous wave laser) to horizontal polarization, and the combined light is expanded by a lens group with a focal length of 25mm and 125mm, and spatially filtered by a pinhole located in the focal plane. Next, a reflective liquid crystal SLM is used for wavefront modulation, and the combined light is reflected by the SLM and focused on the absorber through the lens. The numerical aperture (NA) of the AWA-GR-PAM in this embodiment is 0.1. After the light is absorbed by the absorber, the photoacoustic signal is captured by an ultrasonic transducer with a center frequency of 50MHz, and is collected by a data acquisition card after amplification. The sample was scanned in two dimensions using a motorized translation stage. Figure 3 The time series of the pulsed laser (the laser transmitter in the initial photoacoustic microscopy imaging assembly), the CW laser (continuous wave laser), and the photoacoustic signal are shown. The pulsed laser (the laser transmitter in the initial photoacoustic microscopy imaging assembly) generates a double pulse sequence with a repetition rate of 1 kHz. In each sequence, the first pulse laser is triggered before the CW laser (continuous wave laser) is heated to generate a reference photoacoustic signal, such as Figure 4 The second pulse laser is triggered at the end of CW laser (continuous wave laser) heating to generate a photoacoustic signal with a larger amplitude induced by the GR effect, such as Figure 4 PA2 and PA4 in the figure. The time interval between the two pulses is about 100μs. The intensity modulation frequency of the CW laser (continuous wave laser) is 1kHz, and the heating time is 30μs. Since the thermal relaxation time of the spot is much shorter than the 1ms period of the CW laser, the heat energy generated by the CW laser (continuous wave laser) in each sequence can be dissipated, and the impact on the next sequence is negligible. Between each sequence, such as Figure 4 At time t1, the spatial light modulator SLM loads a series of ordered Zernike polynomials.

[0073] It should be noted that Figure 3In the figure, HWP is a half-wave plate; BS is a beam splitter; PBS is a polarization beam splitter; PD is a photodiode; L1, L2, and L3 are lenses; PH is a pinhole; SLM is a spatial light modulator; UT is an ultrasonic transducer; MRA is a right-angle reflection prism; AMP is an amplifier; and DAQ is a data acquisition card. Figure 4 From top to bottom are the timing diagrams of pulsed laser (laser transmitter in the initial photoacoustic microscopy imaging assembly), CW laser (continuous wave laser), and photoacoustic signal. At time t1, the SLM loads a series of ordered Zernike polynomials to compensate for the optical aberration caused by the absorber, so that PA3>PA1, PA4>PA2, and (PA4-PA3)>(PA2–PA1).

[0074] According to the following Figure 5-Figure 8 The corresponding specific device embodiments are used to illustrate the corresponding effects of the photoacoustic microscopy imaging device provided in the present application.

[0075] This example uses carbon fiber filaments for imaging to verify the effectiveness of AWA-GR-PAM. First, the order of the Zernike polynomial is set to 4 to 10, that is, excluding the piston, x tilt and y tilt. The coefficient step size of each order of the Zernike polynomial is set to πrad, and the optimization range is -3π~3πrads. Figure 5 and Figure 6 These are the maximum amplitude projection (MAP) images after and without AWA-GR correction. By comparing these two MAP images, it can be seen that the normalized signal intensity after AWA-GR correction is generally greater than the normalized signal intensity without AWA-GR correction, and the MAP image after AWA-GR correction shows the characteristics of carbon fiber filaments more clearly. In addition, Figure 7 It is along Figure 5 and Figure 6 The one-dimensional curve plotted by the white dashed line in shows that AWA-GR correction can also enhance image contrast. Figure 8 Shows Figure 5 Phase image at the solid-line box position, which is used to compensate for sample-induced aberrations, shows a normalized signal intensity enhancement of about 2.2 times. These results highlight the advanced capabilities of AWA-GR-PAM in eliminating optical aberrations to provide clear microstructures. Figure 7 The solid and dashed lines represent the normalized intensity distributions after and without AWA-GR correction, respectively.

[0076] In summary, the present application modulates the first pulse laser, the second pulse laser and the continuous wave laser through SLM, and develops AWA-GR-PAM to compensate for the aberration induced by the absorber, and the endogenous absorber acts as a guide star to provide acoustic feedback. According to the above embodiments, when imaging the carbon fiber filaments, it is found that the lateral resolution and signal intensity are improved. The results show that PAM combined with AO can achieve high-quality focusing of the light beam, and the present application can provide insights into the understanding of complex vascular networks.

[0077] The embodiment of the present application also provides a computer device.

[0078] See also Fig. 9 , Fig. 9 is a schematic diagram of the structure of a computer device provided by an optional embodiment of the present application, such as Fig. 9 As shown, the computer device includes: one or more processors 10, a memory 20, and interfaces for connecting various components, including high-speed interfaces and low-speed interfaces. Various components are connected to each other using different buses for communication, and can be installed on a common mainboard or installed in other ways as needed. The processor can process the instructions executed in the computer device, including instructions stored in or on the memory to display the graphical information of the GUI on an external input / output device (such as, a display device coupled to the interface). In some optional embodiments, if necessary, multiple processors and / or multiple buses can be used together with multiple memories and multiple memories. Similarly, multiple computer devices can be connected, and each device provides some necessary operations (for example, as a server array, a group of blade servers, or a multi-processor system). Figure 8 A processor 10 is taken as an example.

[0079] The processor 10 may be a central processing unit, a network processor or a combination thereof. The processor 10 may further include a hardware chip. The hardware chip may be a dedicated integrated circuit, a programmable logic device or a combination thereof. The programmable logic device may be a complex programmable logic device, a field programmable gate array, a general purpose array logic or any combination thereof.

[0080] The memory 20 stores instructions executable by at least one processor 10, so that at least one processor 10 executes the method shown in the above embodiment.

[0081] The memory 20 may include a program storage area and a data storage area, wherein the program storage area may store an operating system, an application required for at least one function; the data storage area may store data created according to the use of the computer device, etc. In addition, the memory 20 may include a high-speed random access memory, and may also include a non-transient memory, such as at least one disk storage device, a flash memory device, or other non-transient solid-state storage device. In some optional embodiments, the memory 20 may optionally include a memory remotely arranged relative to the processor 10, and these remote memories may be connected to the computer device via a network. Examples of the above-mentioned network include, but are not limited to, the Internet, an intranet, a local area network, a mobile communication network, and combinations thereof.

[0082] The memory 20 may include a volatile memory, such as a random access memory; the memory may also include a non-volatile memory, such as a flash memory, a hard disk or a solid state drive; the memory 20 may also include a combination of the above types of memory.

[0083] The computer device further comprises a communication interface 30 for the computer device to communicate with other devices or a communication network.

[0084] The embodiment of the present application also provides a computer-readable storage medium. The above method according to the embodiment of the present application can be implemented in hardware, firmware, or can be implemented as a computer code that can be recorded in a storage medium, or can be implemented as a computer code that is originally stored in a remote storage medium or a non-temporary machine-readable storage medium and will be stored in a local storage medium through a network download, so that the method described herein can be stored in such software processing on a storage medium using a general-purpose computer, a dedicated processor, or programmable or dedicated hardware. Among them, the storage medium can be a magnetic disk, an optical disk, a read-only storage memory, a random access memory, a flash memory, a hard disk or a solid-state hard disk, etc.; further, the storage medium can also include a combination of the above types of memories. It can be understood that a computer, a processor, a microprocessor controller, or programmable hardware includes a storage component that can store or receive software or computer code. When the software or computer code is accessed and executed by a computer, a processor, or hardware, the method shown in the above embodiment is implemented.

[0085] Although the embodiments of the present application are described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present application, and such modifications and variations are all within the scope defined by the appended claims.

Claims

1. An adaptive photoacoustic microscopy imaging method, characterized in that: The invention is applied to an adaptive photoacoustic microscopy imaging device, the device comprising: an initial photoacoustic microscopy imaging component, a continuous wave laser and a spatial light modulator; the method comprising: Controlling the initial photoacoustic microscopy imaging component to emit a first pulse laser and a second pulse laser, and controlling the continuous wave laser to emit a continuous wave laser; The spatial light modulator is controlled to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser; wherein the spatial light modulator uses the differential photoacoustic signal as feedback when performing wavefront modulation.

2. An adaptive photoacoustic microscopy imaging device, characterized in that: For executing the method of claim 1, the device comprises: an initial photoacoustic microscopy imaging component, a continuous wave laser and a spatial light modulator; wherein, The initial photoacoustic microscopy imaging component is used to acquire an image of the target to be imaged according to the first pulse laser and the second pulse laser; The continuous wave laser is used to emit continuous wave laser to the target to be imaged during the imaging process of the initial photoacoustic microscopy imaging assembly to produce the GR effect; The spatial light modulator is used to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser; wherein, when performing wavefront modulation, the spatial light modulator uses the differential photoacoustic signal as feedback.

3. The device according to claim 2, characterized in that The differential photoacoustic signal is obtained according to the following steps: Obtaining the photoacoustic amplitudes of the first pulse laser and the second pulse laser under preset conditions; wherein the preset conditions are: the light flux obeys a two-dimensional Gaussian distribution in the lateral direction; A differential photoacoustic signal between the first pulse laser and the second pulse laser is obtained according to the photoacoustic amplitudes of the first pulse laser and the second pulse laser under preset conditions.

4. The device according to claim 2, characterized in that The initial photoacoustic microscopy imaging assembly includes: a laser transmitter, a laser focusing tool, an ultrasonic transducer and a data processing device.

5. The device according to claim 4, characterized in that The spatial light modulator is used to perform the following steps to perform wavefront modulation on the first pulse laser, the second pulse laser and the continuous wave laser: Acquire a plurality of ordered Zernike polynomials; wherein the Zernike polynomials are used to describe the optical aberrations generated by the initial photoacoustic microscopy imaging component; Loading a plurality of ordered Zernike polynomials into a spatial light modulator in sequence, and determining coefficients corresponding to the plurality of ordered Zernike polynomials; wherein, in the process of determining the coefficients corresponding to the plurality of ordered Zernike polynomials, the differential photoacoustic signal becomes larger; The first pulse laser, the second pulse laser and the continuous wave laser are subjected to wavefront modulation according to coefficients corresponding to a plurality of ordered Zernike polynomials.

6. The device according to claim 5, characterized in that A plurality of ordered Zernike polynomials are sequentially loaded into a spatial light modulator, and coefficients corresponding to the plurality of ordered Zernike polynomials are determined, including: Establishing a feedback loop between the spatial light modulator and the ultrasound transducer and data processing equipment in the initial photoacoustic microscopy imaging assembly; According to the feedback loop, a plurality of ordered Zernike polynomials are traversed to determine coefficients corresponding to the plurality of ordered Zernike polynomials.

7. The device according to claim 5, characterized in that The spatial light modulator is a reflective liquid crystal spatial light modulator.

8. The device according to claim 4, characterized in that The center frequency of the ultrasonic transducer is 50 MHz, and the bandwidth is 31.40-73.25 MHz.

9. The device according to claim 6, characterized in that The order of the Zernike polynomial is 4-10.

10. A computer device, characterized in that: include: A memory and a processor, wherein the memory and the processor are communicatively connected to each other, the memory stores computer instructions, and the processor executes the adaptive photoacoustic microscopy method according to claim 1 by executing the computer instructions.

Images