Toothpaste composition for inhibiting dental plaque and improving gingival inflammation, finished toothpaste and preparation method thereof

Abstract

The invention belongs to the technical field of oral health care, and particularly discloses a toothpaste composition for inhibiting dental plaque and improving gingival inflammation, finished toothpaste and a preparation method thereof. The toothpaste composition is prepared from 0.5 to 10 parts of zinc carbonate hydroxide, 0.01 to 0.2 part of pearl powder, 0.01 to 0.2 part of amber powder, 0.5 to 5 parts of lactobacillus / soybean milk fermentation product filtrate, 0.05 to 1 part of borneol and 0.01 to 0.2 part of clove oil. The toothpaste composition provided by the invention inherits a classic formula, is simple in components and high in safety, and has good effects of inhibiting dental plaque and improving gingival inflammation; and the provided preparation process is simple and easy for large-scale production.

Description

Technical Field

[0001] The invention belongs to the technical field of oral health care, and in particular relates to a toothpaste composition capable of inhibiting dental plaque and improving gingival inflammation, a finished toothpaste and a preparation method thereof. Background Art

[0002] Gingivitis refers to an inflammatory reaction in the gum tissue, which can manifest as swollen, bleeding, and painful gums. However, most Chinese people lack a clear understanding of gum health and do not pay enough attention to it, which leads to missed treatment opportunities, resulting in loose teeth, alveolar bone absorption, and ultimately tooth loss. Therefore, improving gum conditions and improving quality of life have become urgent issues to be addressed.

[0003] Effective toothbrushing is an effective way to improve gingivitis. The main ingredients of ordinary toothpaste include abrasives, detergents, wetting agents, preservatives, and fragrances, which generally only have the function of cleaning the mouth. Medicated toothpaste is based on ordinary toothpaste with specific drugs added. When brushing teeth, the toothpaste reaches the surface of the teeth or the environment around the teeth. Through the action of the drug, it reduces dental plaque and relieves inflammation, thereby improving gingivitis. Medicated toothpaste is very popular among people, and using toothpaste to promote drugs with preventive or therapeutic effects is an ideal way. For example, Sensodyne anti-sensitivity toothpaste, by adding strontium acetate or potassium nitrate to relieve the problem of dentin sensitivity, there are also a variety of toothpastes with added Chinese herbal ingredients, such as Yunnan Baiyao toothpaste, Liangmianzhen toothpaste, etc. In the prior art, patent document CN102698122A provides a traditional Chinese medicine, preparation method, toothpaste and administration method for treating periodontitis. The traditional Chinese medicine composition is composed of wolfberry, donkey-hide gelatin, Adenophora australis, Ophiopogon japonicus, Cornus officinalis, tortoise shell, Drynaria fortunei, Cuscuta australis, ochre, gypsum, Anemarrhena asphodeloides, Poria cocos, Coix seeds, Scutellaria baicalensis, Astragalus membranaceus and licorice. The ingredients are complex, easy to cause allergies, and easy to produce use dependence.

[0004] Based on this, developing a medicated toothpaste that can effectively inhibit dental plaque and improve gingivitis has great market prospects. Summary of the invention

[0005] In view of the deficiencies in the prior art, one of the main purposes of the present invention is to provide a toothpaste composition for improving gingivitis, which inherits the classic formula, has simple ingredients, high safety, and has good effects of inhibiting dental plaque and improving gingivitis.

[0006] The second main purpose of the present invention is to provide a use of the above toothpaste composition in the preparation of a finished toothpaste, which can improve gum health and has a simple preparation process.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a toothpaste composition for inhibiting dental plaque and improving gingival inflammation, which is prepared from the following raw materials in parts by weight:

[0009]

[0010] In the above technical solution, zinc carbonate hydroxide can adjust the pH environment of the oral cavity, inhibit the growth of caries-causing bacteria, and also inhibit the growth of local staphylococci, and has good antiseptic, astringent and protective effects on skin inflammation and surface trauma. Pearl powder has the effects of calming the mind, clearing away heat and nourishing yin, detoxifying and promoting tissue regeneration, and can be used to resolve toxicity, transform malignant sores, and heal internal ulcers; amber is a hydrocarbon formed by the resin of ancient pine plants buried underground for a long time, and has the effects of calming the mind, dispersing blood stasis and stopping bleeding, treating carbuncle sores, trauma, and stopping bleeding and promoting tissue regeneration; pearl powder and amber powder can astringe and protect the wound surface and improve the gum condition.

[0011] Lactobacillus / soy milk fermentation product filtrate improves oral health by inhibiting the synthesis of extracellular polysaccharides by Streptococcus mutans and weakening the adhesion of cariogenic bacteria to form biofilm. It can effectively reduce gingival inflammation, reduce the formation of dental plaque, and effectively regulate the pH balance in the oral cavity and improve breath.

[0012] Borneol has good antibacterial and anti-inflammatory effects, can increase the permeability of human capillaries, and eliminate various inflammations in the patient's body. Borneol also has the effects of clearing heat, relieving pain and promoting tissue regeneration, and can relieve gum pain.

[0013] Clove oil is a natural plant essential oil with broad spectrum antibacterial, analgesic and anti-inflammatory effects. It has a strong inhibitory effect on a variety of caries-causing bacteria. It is rich in antioxidants, which can inhibit inflammatory responses in the mouth and reduce redness, swelling and pain symptoms of the oral mucosa.

[0014] As a preferred embodiment of the above technical solution, the toothpaste composition is prepared from the following raw materials in parts by weight:

[0015]

[0016] As a further preferred embodiment of the above technical solution, the toothpaste composition is prepared from the following raw materials in parts by weight:

[0017]

[0018] In a second aspect, the present invention provides a use of the toothpaste composition in preparing toothpaste.

[0019] In a third aspect, more specifically, the present invention provides a toothpaste for inhibiting dental plaque and improving gingival inflammation, which is prepared from the above toothpaste composition and necessary auxiliary materials.

[0020] As a further preference of the above technical solution, the auxiliary materials used in the above toothpaste product are selected from one or more of moisturizers, abrasives, thickeners, adhesives, surfactants, preservatives, sweeteners, flavors, and purified water.

[0021] As a further preferred embodiment of the above technical solution, the dosage of each auxiliary material is as follows: 5-50 parts of moisturizing agent, 5-30 parts of abrasive, 1-10 parts of thickener, 0.1-5 parts of adhesive, 0.5-5 parts of surfactant, 0.1-1 parts of preservative, 0.05-0.5 parts of sweetener, and 0.01-0.1 parts of flavor.

[0022] As a further preferred embodiment of the above technical solution, the humectant is selected from one or more of glycerol, sorbitol, 1,3-propylene glycol, and polyethylene glycol;

[0023] The friction agent is selected from one or more of calcium carbonate, calcium hydrogen phosphate dihydrate, precipitated silica, and aluminum hydroxide;

[0024] The thickener is selected from one or more of thickening silicon dioxide, xanthan gum, and sodium carboxymethyl cellulose;

[0025] The adhesive is selected from one or both of cellulose gum and sodium alginate;

[0026] The surfactant is selected from one or more of sodium lauryl sulfate, sodium lauroyl sarcosinate, and sodium dodecyl sulfate;

[0027] The preservative is selected from one or more of phenoxyethanol, potassium sorbate, and sodium benzoate;

[0028] The sweetener is selected from one or more of saccharin sodium, galactose trichloride and xylitol.

[0029] In a fourth aspect, the present invention further provides a method for preparing the above toothpaste, comprising the following steps:

[0030] (1) mixing pearl powder, amber powder and zinc carbonate hydroxide, grinding and sieving to obtain natural mineral powder;

[0031] (2) mixing the lactobacillus / soymilk fermentation product filtrate, clove oil and the natural mineral powder obtained in step (1), then adding abrasives, moisturizers, sweeteners, preservatives, flavors and borneol, and stirring evenly to obtain a mixture A;

[0032] (3) stirring and dispersing the thickener and the binder in deionized water to obtain a mixture B;

[0033] (4) Mixing the mixture A obtained in step (2) and the mixture B obtained in step (3) and stirring them, then slowly adding a surfactant and continuing to stir until uniform.

[0034] As a further preferred embodiment of the above technical solution, in step (1), the product is sieved through 200 mesh.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) The toothpaste composition provided by the present invention inherits the classic formula of the applicant's Babao Hemorrhoid Cream product. By adding natural mineral ingredients such as zinc carbonate hydroxide, pearl powder and amber powder, and combining with multi-strain fermentation products obtained by modern fermentation technology, the multi-component synergistic effect regulates the oral pH environment and microecological balance, improves gingival inflammation, thereby inhibiting the growth of caries-causing bacteria, inhibiting dental plaque, relieving gingival bleeding, and playing a role in astringency and protection of the wound surface; further adding borneol and clove oil that relieve gum pain, inhibits dental plaque, improves gingival inflammation, and improves the health of gums in multiple dimensions.

[0037] (2) The toothpaste composition provided by the present invention has a major innovation in that natural mineral ingredients such as zinc carbonate hydroxide, pearl powder and amber powder are added, which expands the new application uses of the above substances; the various substances are used in combination with each other, the raw material ingredients are simple, the effect of improving dental health problems is significant, and it has a good market application prospect.

[0038] (3) The toothpaste composition provided by the present invention further adjusts the dosage of the natural mineral components zinc carbonate hydroxide, pearl powder and amber powder in the toothpaste composition. The natural mineral components exert a better anti-inflammatory effect under specific ratio conditions, thereby better improving the symptoms of gingival swelling, bleeding and pain.

[0039] (4) The toothpaste preparation method provided by the present invention has a simple process and is suitable for industrial large-scale production. DETAILED DESCRIPTION

[0040] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Unless otherwise specified, all commodities or reagents in this invention are purchased through market channels. Some raw material information is as follows:

[0042] The lactobacillus / soy milk fermentation product filtrate used in the present invention was purchased from France's Cintin, with the trade name ORAL (INCI: Aqua, Lactobacillus / Soymilk Ferment Filtrate, Butylene Glycol, Pentylene Glycol).

[0043] Example 1

[0044] A toothpaste for inhibiting dental plaque and improving gingival inflammation, which is made of the following raw materials by weight:

[0045] Table 1 Example 1 Raw material composition

[0046]

[0047]

[0048] In this embodiment, a method for preparing the toothpaste is also provided, comprising the following steps:

[0049] (1) grinding pearl powder, amber powder and zinc carbonate hydroxide, and passing through a 200-mesh sieve to obtain natural mineral powder;

[0050] (2) mixing the lactobacillus / soy milk fermentation product filtrate, clove oil and the natural mineral powder obtained in step (1), adding an abrasive (calcium hydrogen phosphate dihydrate), a moisturizer (sorbitol), a sweetener (saccharin sodium), a preservative (sodium benzoate), a flavor and borneol, and stirring evenly to obtain a mixture A;

[0051] (3) stirring and dispersing a thickener (thickening silica) and a binder (cellulose gum) in deionized water (i.e., purified water, the same below) to obtain a mixture B;

[0052] (4) Mixture A and mixture B are mixed and stirred, and then a surfactant (sodium lauryl sulfate) is slowly added under stirring, and vacuum stirring is continued until the mixture is uniform to obtain a toothpaste paste.

[0053] Example 2

[0054] A toothpaste for inhibiting dental plaque and improving gingival inflammation, which is made of the following raw materials by weight:

[0055] Table 2 Example 2 Raw material composition

[0056]

[0057]

[0058] In this embodiment, a method for preparing the toothpaste is also provided, comprising the following steps:

[0059] (1) grinding pearl powder, amber powder and zinc carbonate hydroxide, and passing through a 200-mesh sieve to obtain natural mineral powder;

[0060] (2) mixing the lactobacillus / soy milk fermentation product filtrate, clove oil and the natural mineral powder obtained in step (1), adding an abrasive (precipitated silica), a moisturizer (sorbitol), a sweetener (saccharin sodium), a preservative (sodium benzoate), a flavor and borneol, and stirring evenly to obtain a mixture A;

[0061] (3) stirring and dispersing a thickener (sodium carboxymethyl cellulose) and a binder (cellulose gum) in deionized water to obtain a mixture B;

[0062] (4) Mixture A and mixture B are mixed and stirred, and then a surfactant (sodium lauryl sulfate) is slowly added under stirring, and vacuum stirring is continued until the mixture is uniform to obtain a toothpaste paste.

[0063] Example 3

[0064] A toothpaste for inhibiting dental plaque and improving gingival inflammation, which is made of the following raw materials by weight:

[0065] Table 3 Example 3 Raw material composition

[0066]

[0067]

[0068] In this embodiment, a method for preparing the toothpaste is also provided, comprising the following steps:

[0069] (1) grinding pearl powder, amber powder and zinc carbonate hydroxide, and passing through a 200-mesh sieve to obtain natural mineral powder;

[0070] (2) mixing the lactobacillus / soy milk fermentation product filtrate, clove oil and the natural mineral powder obtained in step (1), adding an abrasive (calcium carbonate), a moisturizer (polyethylene glycol), a sweetener (saccharin sodium), a preservative (phenoxyethanol), a flavor and borneol, and stirring evenly to obtain a mixture A;

[0071] (3) stirring and dispersing a thickener (thickening silica) and a binder (cellulose gum) in deionized water to obtain a mixture B;

[0072] (4) Mixture A and mixture B are mixed and stirred, and then a surfactant (sodium lauroyl sarcosinate) is slowly added under stirring, and vacuum stirring is continued until uniform to obtain a toothpaste paste.

[0073] Comparative Example 1

[0074] Compared with Example 1, the difference is that the amber powder is omitted in Comparative Example 1 and replaced with an equal amount of purified water, and the other raw materials and preparation methods are the same.

[0075] Comparative Example 2

[0076] Compared with Example 1, the difference is that in Comparative Example 2, the use of lactobacillus / soymilk fermentation product filtrate is omitted and replaced with an equal amount of purified water, and the other raw materials and preparation methods are the same.

[0077] Comparative Example 3

[0078] Compared with Example 1, the difference is that in Comparative Example 3, the use of zinc carbonate hydroxide is omitted and replaced by an equal amount of purified water, and the other raw materials and preparation methods are the same.

[0079] Comparative Example 4

[0080] Compared with Example 1, the difference is that in Comparative Example 4, pearls are omitted and replaced with an equal amount of purified water, and the other raw materials and preparation methods are the same.

[0081] Comparative Example 5

[0082] Compared with Example 1, the difference is that in Comparative Example 5, amber powder and zinc carbonate hydroxide are omitted and replaced with an equal amount of purified water, and the other raw materials and preparation methods are the same.

[0083] Test Example 1

[0084] Example 1 and Comparative Examples 1 to 5 were evaluated, and the inhibition rate of Streptococcus mutans biofilm at different treatment times was determined by crystal violet (CV) staining. The specific method is as follows:

[0085] The toothpastes prepared in Example 1 and Comparative Examples 1 to 5 of the present invention were prepared into a 1 g / L test solution for later use. Streptococcus mutans (Streptococcus mutans CGMCC 12499; China General Microorganism Collection Center) was inoculated into a BHI liquid culture medium, anaerobically cultured at 37° C. for 24 hours, and an appropriate amount of the culture solution was mixed with the BHI liquid culture medium to prepare a 5% (volume fraction) mixed solution of Streptococcus mutans, and then anaerobically cultured at 37° C. for 15 hours to obtain a Streptococcus mutans solution for later use.

[0086] Take a sterile 96-well plate, add mutans streptococcus solution to each well and culture for 24 hours to form a mature biofilm of mutans streptococcus, aspirate the supernatant, add the test solution and treat for 1min, 10min, 30min, and 60min respectively, and set up a blank control group. After staining the biofilm in each well with the CV staining method, the decolorization solution is used to detect the absorbance value of mutans streptococcus at 600nm (OD600nm) with a UV-visible spectrophotometer. The above experiment was effectively repeated 3 times, and the OD value test results of each group of the experiment were averaged, and the product's inhibition rate on biofilm was calculated according to the following formula:

[0087] Biofilm inhibition rate (%) = [(blank control OD value - experimental group OD value) / blank control OD value] × 100%.

[0088] The results of the inhibition rates of mutans streptococcus biofilms treated with the products obtained in the embodiments and comparative examples for 1 min, 10 min, 30 min and 60 min respectively are shown in Table 4.

[0089] Table 4 Results of inhibition rate of Streptococcus mutans biofilm

[0090]

[0091] Test Example 2

[0092] The toothpastes prepared in Example 1 and Comparative Examples 1 to 5 were used as test products to conduct a human efficacy evaluation test to evaluate the overall oral health of the products.

[0093] 2.1 Evaluation indicators

[0094] (1) Overall oral health - plaque index

[0095] The supragingival plaque was stained and scored according to the following criteria: 0 means no plaque; 1 means scattered plaque at the edge of the tooth neck; 2 means a continuous thin plaque band (up to 1 mm wide) can be seen at the edge of the tooth neck; 3 means the plaque band at the tooth neck is larger than 1 mm but less than 1 / 3 of the tooth surface; 4 means the plaque covers 1 / 3 to 2 / 3 of the tooth surface; 5 means the plaque covers more than 2 / 3 of the tooth surface.

[0096] Plaque index (PLI) = the sum of the scores of all teeth examined for each subject / number of teeth measured (number of teeth × 2).

[0097] (2) Overall oral health - Gum index

[0098] The gingival index (GI) was measured by the modified Loe-Silness method. The gingivitis scoring method and standard were as follows: 0 for no inflammation; 1 for mild inflammation, with slight changes in gingival color and texture, but no bleeding on probing; 2 for moderate inflammation, with shiny, red, swollen, and hyperplastic gingiva, and bleeding on probing; 3 for severe inflammation, with obvious redness and hyperplasia of the gingiva, and spontaneous bleeding.

[0099] Gingival index (GI) = the sum of all tooth scores / number of measured tooth surfaces.

[0100] 2.2 Test methods

[0101] 60 healthy Chinese subjects aged 19 to 60 were selected and randomly divided into 6 groups, 10 in each group. They used the test product for 28 consecutive days, brushed their teeth twice a day for 2 minutes each time, and had a washout period of 1 week. All subjects were required to be evaluated from 8:00 to 12:00 in the morning on the 0th, 14th and 28th days, and did not brush their teeth or eat anything after waking up; the data were then statistically analyzed using SPSS software.

[0102] 2.3 Test results

[0103] 2.3.1 Plaque Index (PLI)

[0104] The results of the plaque index (PLI) obtained by the test are shown in Tables 5 and 6. As shown in Table 5, after using the test product for 2 weeks, the plaque index decreased by 9.54%, and after using the test product for 4 weeks, the plaque index decreased significantly by 23.65% (P < 0.01), indicating that the product has excellent plaque removal effect, and the plaque removal effect of the product of the embodiment of the present invention is much better than that of the comparative product.

[0105] Table 5 Plaque index results

[0106]

[0107]

[0108] (Compared with before using the product, *P<0.05, **P<0.01)

[0109] Table 6 Results of plaque index improvement rate

[0110]

[0111] 2.3.2 Gingival Index (GI)

[0112] The gingival index (GI) results obtained by the test are shown in Tables 7 and 8. As shown in Table 7, after using the test product for 2 weeks, the gingival index decreased by 14.29%, and after using the test product for 4 weeks, the gingival index decreased significantly by 37.01% (P < 0.01), indicating that the product has excellent efficacy in improving gingival inflammation, and the product of the embodiment of the present invention is much better than the comparative product in improving gingival inflammation.

[0113] Table 7 Gingival index results

[0114]

[0115] (Compared with before using the product, *P<0.05, **P<0.01)

[0116] Table 8 Results of improvement rate of gingival index

[0117]

[0118]

[0119] 2.3.3 Overall satisfaction survey results

[0120] After using the test product for 4 weeks, the subjects were asked to fill out a questionnaire with options including strongly agree, somewhat agree, neither agree nor disagree, somewhat disagree and strongly disagree, and the percentage of subjects who strongly agreed and somewhat agreed was calculated. The results of the overall satisfaction survey of the subjects after using the product for 4 weeks are shown in Table 9.

[0121] Table 9 Overall satisfaction survey results

[0122] Test example Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Overall better oral health 100％ 80％ 50％ 70％ 90％ 60％ Less breath 100％ 70％ 40％ 70％ 90％ 50％ Reduced gum bleeding 100％ 80％ 50％ 70％ 100％ 60％ Reduce gum redness and swelling 100％ 90％ 50％ 80％ 90％ 60％ Reduced tooth sensitivity 90％ 50％ 30％ 60％ 80％ 40％ Overall product satisfaction 100％ 70％ 60％ 70％ 90％ 50％

[0123] It can be seen from Table 9 that after the subjects used the product for 28 days, 100% of the subjects were satisfied with the gum improvement effect of the example product (including very satisfied and relatively satisfied), while the satisfaction with the gum improvement effect of the products in Comparative Examples 1 to 5 was relatively poor. All subjects did not experience adverse reactions or discomfort during the use of the product, and the product is highly safe and non-irritating.

Claims

1. A toothpaste composition for inhibiting dental plaque and improving gingival inflammation, characterized in that: In parts by weight, it is prepared from the following raw materials:

2. A toothpaste composition for inhibiting dental plaque and improving gingival inflammation according to claim 1, characterized in that: In parts by weight, it is prepared from the following raw materials:

3. A toothpaste composition for inhibiting dental plaque and improving gingival inflammation according to claim 1, characterized in that: In parts by weight, it is prepared from the following raw materials:

4. Use of the toothpaste composition according to any one of claims 1 to 3 in the preparation of toothpaste.

5. A toothpaste for inhibiting dental plaque and improving gingivitis, characterized in that: The toothpaste is prepared from the toothpaste composition according to any one of claims 1 to 3 and auxiliary materials.

6. The toothpaste according to claim 5, characterized in that The auxiliary material is selected from one or more of a moisturizer, a friction agent, a thickener, a binder, a surfactant, a preservative, a sweetener, a flavor, and purified water.

7. The toothpaste according to claim 6, characterized in that The dosage of each auxiliary material is as follows: 5-50 parts of moisturizing agent, 5-30 parts of friction agent, 1-10 parts of thickener, 0.1-5 parts of adhesive, 0.5-5 parts of surfactant, 0.1-1 parts of preservative, 0.05-0.5 parts of sweetener, 0.01-0.1 parts of flavor.

8. The toothpaste according to claim 6, characterized in that The moisturizer is selected from one or more of glycerol, sorbitol, 1,3-propylene glycol, and polyethylene glycol; The friction agent is selected from one or more of calcium carbonate, calcium hydrogen phosphate dihydrate, precipitated silica, and aluminum hydroxide; The thickener is selected from one or more of thickening silicon dioxide, xanthan gum, and sodium carboxymethyl cellulose; The adhesive is selected from one or both of cellulose gum and sodium alginate; The surfactant is selected from one or more of sodium lauryl sulfate, sodium lauroyl sarcosinate, and sodium dodecyl sulfate; The preservative is selected from one or more of phenoxyethanol, potassium sorbate, and sodium benzoate; The sweetener is selected from one or more of saccharin sodium, galactose trichloride and xylitol.

9. A method for preparing the toothpaste according to any one of claims 5 to 9, characterized in that: The steps include: (1) mixing pearl powder, amber powder and zinc carbonate hydroxide, grinding and sieving to obtain natural mineral powder; (2) mixing the lactobacillus / soymilk fermentation product filtrate, clove oil and the natural mineral powder obtained in step (1), then adding abrasives, moisturizers, sweeteners, preservatives, flavors and borneol, and stirring evenly to obtain a mixture A; (3) stirring and dispersing the thickener and the binder in deionized water to obtain a mixture B; (4) Mixing the mixture A obtained in step (2) and the mixture B obtained in step (3) and stirring them, then slowly adding a surfactant and continuing to stir until uniform.

10. The preparation method according to claim 9, characterized in that: In step (1), the product is sieved through 200 mesh.

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