Anti-depression traditional Chinese medicine formula and application thereof

Abstract

The invention discloses an anti-depression traditional Chinese medicine formula and application thereof, and belongs to the field of medicines, and the traditional Chinese medicine formula comprises the following raw materials in parts by weight: 4-12 parts of radix bupleuri, 2-6 parts of polygala tenuifolia, 2-6 parts of ginseng, 2-8 parts of poria cocos, 2-8 parts of radix paeoniae alba, 3-6 parts of acorus calamus, 4-8 parts of bighead atractylodes rhizome, 4-8 parts of angelica sinensis, 3-9 parts of cape jasmine, 3-9 parts of rhizoma atractylodis, 2-8 parts of medicated leaven and 2-8 parts of liquorice. The traditional Chinese medicine composition can improve the anxiety degree and despair state of LPS mouse behavioristics, which shows that the traditional Chinese medicine composition has the effects of resisting depression, resisting anxiety and improving mental state. The whole formula is reasonable and rigorous, various dosage forms of medicines, substitutional tea and beverages for treating depression can be prepared, the raw materials are easy to obtain, the preparation process is relatively simple, and the cost can be controlled.

Description

Technical Field

[0001] The invention belongs to the field of medicine, and specifically relates to an anti-depressant traditional Chinese medicine prescription and application thereof. Background Art

[0002] Depression, a global mental health challenge, is increasingly becoming a focus of public health attention. Its core feature is a significant and persistent low mood, which not only affects the patient's daily life functions, but may also lead to social isolation, cognitive impairment and even suicidal tendencies, placing a heavy burden on individuals, families and even society. With the accelerated pace of life and the increase in social pressure, the number of people suffering from depression worldwide is rising year by year, becoming an important health problem that needs to be addressed urgently.

[0003] The pathogenesis of depression is complex and has not yet been fully elucidated. Current research generally believes that the occurrence of depression is the result of the combined effects of multiple factors, covering multiple levels such as biology, psychology, and social environment. At the biological level, the imbalance of neurotransmitters (such as serotonin, norepinephrine, etc.), the disorder of the microbiome-brain-gut axis, the dysfunction of the neuroendocrine system, and the involvement of inflammatory responses and cytokines are all considered to be the key mechanisms for the onset of depression. The interaction of these mechanisms forms a complex and subtle network that affects the individual's emotional regulation and psychological state.

[0004] For the treatment of depression, the Western medicine system mainly relies on drug therapy, electroconvulsive therapy and surgical treatment. In drug therapy, antidepressants occupy a core position, including but not limited to serotonin reuptake inhibitors (SSRI), selective serotonin and norepinephrine reuptake inhibitors (SNRI), norepinephrine and specific serotonergic antidepressants (NaSSA), serotonin 2A receptor antagonists and serotonin reuptake inhibitors (SARIs), monoamine oxidase inhibitors (MAOI), etc. Although these drugs can relieve depressive symptoms to a certain extent, their side effects cannot be ignored, such as inducing mania, affecting sleep quality, and causing dependence. In addition, some patients do not respond well to drug treatment, and there are large individual differences in efficacy.

[0005] In addition, although electroconvulsive therapy and surgical treatment are effective for some patients with severe depression, they are often not the first choice of treatment due to the physical pain and psychological pressure they bring. Therefore, it is particularly important to find a safer, more effective and less side-effect antidepressant method.

[0006] In this context, traditional Chinese medicine, with its unique theoretical system and treatment concept, provides a new approach to the treatment of depression. Traditional Chinese medicine emphasizes the holistic concept and treatment based on syndrome differentiation. It believes that the occurrence of depression is related to factors such as dysfunction of internal organs and emotional injury. It achieves the purpose of treatment by adjusting the balance of yin and yang, harmonizing qi and blood, and relieving liver depression. Numerous clinical practices and studies have shown that some single Chinese medicines or compound prescriptions (such as Polygala tenuifolia, Bupleurum chinense, Kaixinsan, Ganmai Dazao Decoction, etc.) have significant therapeutic effects in improving depressive symptoms, and the side effects are relatively small, which reflects the unique advantages of traditional Chinese medicine in the treatment of depression.

[0007] In view of this, combining the multi-dimensional and multi-target treatment characteristics of traditional Chinese medicine, we seek a new antidepressant Chinese medicine formula, aiming to effectively relieve depressive symptoms and improve patients' quality of life by comprehensively regulating human functions and restoring the balance of yin and yang, providing a new option for the treatment of depression. Summary of the invention

[0008] In order to solve the existing technical problems and clinical needs, the present invention proposes an antidepressant Chinese medicine formula and its application, the raw materials are easily available, the preparation is convenient, and it can be used to treat depression.

[0009] To achieve the above object, the present invention adopts the following technical solutions:

[0010] An antidepressant Chinese medicine prescription, the Chinese medicine prescription is composed of the following raw materials in parts by weight:

[0011] 4-12 parts of Bupleurum, 2-6 parts of Polygala, 2-6 parts of Ginseng, 2-8 parts of Poria, 2-8 parts of White Peony, 3-6 parts of Acorus, 4-8 parts of Atractylodes, 4-8 parts of Angelica, 3-9 parts of Gardenia, 3-9 parts of Atractylodes, 2-8 parts of Shenqu, and 2-8 parts of Licorice.

[0012] Preferably, the Chinese medicine formula is composed of the following raw materials in parts by weight:

[0013] 6 parts of Bupleurum, 3 parts of Polygala, 3 parts of Ginseng, 6 parts of Poria, 6 parts of White Peony, 3 parts of Calamus, 6 parts of Atractylodes, 6 parts of Angelica, 6 parts of Gardenia, 6 parts of Atractylodes, 6 parts of Shenqu, 6 parts of Liquorice. The combination of Bupleurum, Polygala, Acorus, Poria, Ginseng, White Peony, Atractylodes, and Liquorice can relieve liver depression, invigorate Qi and nourish the heart; the combination of Angelica, Gardenia, Atractylodes, and Shenqu can regulate Qi and relieve depression, relieve fullness in the middle.

[0014] The Chinese medicine formula of the present invention can be prepared into decoction, pills, dripping pills, granules, tablets, hard capsules, soft capsules, decoctions, syrups, oral liquids or beverages.

[0015] The present invention also provides a method for preparing the antidepressant Chinese medicine formula, which comprises mixing the raw materials according to weight, soaking and then decocting, and taking the decocted medicinal liquid to obtain a finished Chinese medicine.

[0016] Specifically, take each raw material, add 10 times the amount of water, reflux extraction twice, each time for 1 hour, combine the decoctions, filter, and concentrate under reduced pressure. The process parameters are: 0.08-0.10Mpa, rotation speed 90rpm, 60°C, to prepare the finished Chinese medicine.

[0017] The present invention also provides application of the antidepressant traditional Chinese medicine formula in preparing antidepressant traditional Chinese medicine products.

[0018] Specifically, the Chinese medicine product is a decoction, pills, dripping pills, granules, tablets, hard capsules, soft capsules, decoctions, syrups, oral liquids or beverages.

[0019] When the present invention is used, the dosage of the traditional Chinese medicine product is 5-20g / kg.

[0020] The present invention can improve the anxiety level and despair state of the behavior of LPS mice, indicating that the Chinese medicine formula of the present invention has the effects of anti-depression, anti-anxiety and improving mental state. The whole formula of the present invention is reasonable and rigorous, and can be prepared into various dosage forms of drugs, substitute teas and beverages for treating depression. The raw materials are easily available, the preparation process is relatively simple, and the cost can be controlled. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a picture of the preparation of the water extract of the Chinese medicine compound according to the present invention.

[0022] Figure 2 The figure shows the effect of the present invention on the forced swimming test of the mouse depression model prepared by intraperitoneal injection of lipopolysaccharide (LPS); compared with the model group, * indicates p<0.05, *** indicates p<0.001; compared with the control group, # indicates p<0.05.

[0023] Figure 3 The figure shows the effect of the present invention on the tail suspension experiment of the mouse depression model prepared by intraperitoneal injection of LPS; compared with the model group, * indicates p<0.05, ** indicates p<0.01; compared with the control group, # indicates p<0.05.

[0024] Figure 4 The figure shows the effect of the present invention on the open field experiment of the mouse depression model prepared by intraperitoneal injection of LPS; wherein, compared with the control group, ## indicates p<0.01.

[0025] Figure 5 This is a diagram showing the movement trajectories of mice in different groups in an open field experiment of a mouse depression model prepared by intraperitoneal injection of LPS in the present invention. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Example 1 Preparation of aqueous extract of Chinese medicine formula

[0028] Prescription: Bupleurum 6g, Polygala 3g, Ginseng 3g, Poria 6g, White Peony 6g, Acorus 3g, Atractylodes 6g, Angelica 6g, Gardenia 6g, Atractylodes 6g, Shenqu 6g, Licorice 6g.

[0029] Preparation: Take each raw material, add 10 times the amount of water, reflux extraction twice, each time for 1 hour, combine the decoction, filter, and concentrate under reduced pressure. The process parameters are: 0.08-0.10Mpa, speed 90r / min, 60℃, and prepare three concentrations of water extract: low, medium and high. The crude drug dosage is 0.5g / ml, 1g / ml and 2g / ml respectively. Figure 1 ).

[0030] Example 2

[0031] Prescription: Bupleurum 4g, Polygala 2g, Ginseng 2g, Poria 2g, White Peony 6g, Acorus 3g, Atractylodes 4g, Angelica 4g, Gardenia 3g, Atractylodes 3g, Shenqu 2g, Licorice 2g.

[0032] Preparation: Take each raw material, add 10 times the amount of water, reflux extraction twice, 1 hour each time, combine the decoctions, filter, and concentrate under reduced pressure. The process parameters are: 0.08-0.10Mpa, speed 90r / min, 60℃, to prepare the water extract.

[0033] Example 3

[0034] Prescription: Bupleurum 12g, Polygala 6g, Ginseng 6g, Poria 8g, White Peony 8g, Acorus 6g, Atractylodes 8g, Angelica 8g, Gardenia 9g, Atractylodes 9g, Shenqu 8g, Licorice 8g.

[0035] Preparation: Take each raw material, add 10 times the amount of water, reflux extraction twice, 1 hour each time, combine the decoctions, filter, and concentrate under reduced pressure. The process parameters are: 0.08-0.10Mpa, speed 90r / min, 60℃, to prepare the water extract.

[0036] Example 4 Establishment of experimental animal depression model

[0037] 1. Experimental animals

[0038] SPF-grade C57BL / 6 mice, male, weighing 19.0-22.0 g, were purchased from Zhejiang Weitong Lihua Experimental Animal Technology Co., Ltd. with license number SCXK(Zhejiang)2020-0002 and were kept in the SPF-grade laboratory of the Experimental Animal Center of Nanhu Brain-Computer Cross-Research Institute with a relative temperature of 23±2°C and a relative humidity of 45%-65%. The feed was regular feed, and the animals were free to eat and drink water.

[0039] (II) Experimental plan

[0040] 1. Animal Grouping

[0041] 60 C57BL / 6 mice were randomly divided into 6 groups, 10 mice in each group, namely, a control group, a model group, a positive drug group, and low-, medium- and high-dose groups of the aqueous extract of the traditional Chinese medicine formula prepared in Example 1 of the present invention.

[0042] 2. Dosage regimen

[0043] The mice were intragastrically administered with 10 ml / kg per mouse per day. The positive drug group was given 5 mg / kg of fluoxetine hydrochloride (FluoxetineHCl), the high-dose group of the water extract prepared in Example 1 of the present invention was given 20 g / kg (abbreviated as ZY 20 g / kg), the medium-dose group of the granules prepared in Example 1 of the present invention was given 10 g / kg (abbreviated as ZY 10 g / kg), the low-dose group of the granules prepared in Example 1 of the present invention was given 5 g / kg (abbreviated as ZY 5 g / kg), and the control group (Control) and the model group were given the same volume of distilled water for 14 consecutive days.

[0044] 3. Depression Model Establishment

[0045] After 14 consecutive days of administration, LPS was dissolved in sterile saline to a concentration of 0.1 mg / ml for later use. The control group was intraperitoneally injected with sterile saline on days 14-16, with an injection volume of 10 ml / kg (equivalent to a dosage of 1 mg / kg). The other five groups were intraperitoneally injected with 10 ml / kg of LPS saline solution on days 14-16.

[0046] Example 5 Experimental Animal Forced Swim Test (FST)

[0047] 1. Experimental Protocol

[0048] According to Example 4, 0.5 h after the end of dosing on the 16th day, the mice were placed in a transparent water bucket with a depth of 20 cm and a diameter of 40 cm, the water temperature was maintained at 25°C, and pre-adapted for 2 min. The immobility time within the next 4 min was recorded.

[0049] 2. Experimental results

[0050] The results showed that the swimming immobility time of the mice in the model group was significantly increased compared with that in the control group (P<0.05), indicating that the mice showed obvious despair behavior after modeling, indicating that the modeling was successful. The water extract of the Chinese medicine formula prepared in Example 1 of the present invention at different doses showed antidepressant effects in the forced swimming test, and in the forced swimming test, the low, medium and high dose groups had significant differences compared with the model group (P<0.05), as shown in Table 1. Figure 2 .

[0051] Example 6 Experimental Animal Tail Suspension Test (TST)

[0052] 1. Experimental Protocol

[0053] Use medical tape to stick it on the tail of the mouse 1 cm away from the tip of the tail, and hang it 15 cm above the table surface. Allow the mouse to pre-adapt for 2 minutes, and record the immobility time of the mouse within the next 4 minutes.

[0054] 2. Experimental results

[0055] Compared with the control group, the tail suspension immobility time of the model group mice was significantly increased compared with the control group (P<0.05), indicating that the mice showed obvious despair behavior after modeling, indicating that the modeling was successful. The water extract of the Chinese medicine formula prepared in Example 1 of the present invention at different doses showed an antidepressant effect in the tail suspension experiment, and in the tail suspension experiment, the immobility time of the low and medium dose groups showed a downward trend compared with the model group, and the high dose group was significantly higher than the model group (P<0.05), indicating that the drug played a role in depressive-like behavior results, see Figure 3 .

[0056] Example 7 Experimental Animal Open Field Test (OFT)

[0057] 1. Experimental Protocol

[0058] The mice were placed in a 30×30 cm white square frame and pre-adapted for 2 min. The time spent in the center area and the proportion of movement distance in the center area were recorded for the next 4 min.

[0059] 2. Experimental results

[0060] In the open field experiment, the medium-dose group was selected as a representative. Compared with the control group, the time spent in the center of the open field and the proportion of the distance moved in the center of the open field in the model group were significantly reduced (P<0.05), indicating that the anxiety level of the mice increased after modeling, indicating that the modeling was successful. Compared with the model group, the time spent in the center of the open field and the proportion of the distance moved in the center of the open field in the medium-dose group showed an upward trend ( Figure 4 and Figure 5 ), indicating that the drug resulted in depressive-like behavior.

[0061] The experimental results showed that after LPS modeling, the swimming immobility time, tail suspension immobility time, time spent in the center of the open field, and the proportion of movement distance in the center of the open field in each model group were significantly reduced compared with the blank group, indicating that the depression model was successful. The results of behavioral index determination showed that, in general, the low, medium, and high dose groups prepared in Example 1 of the present invention can improve the depressive-like behavior of LPS mice.

[0062] In summary, the aqueous extract of the Chinese medicine formula prepared by the present invention can improve the anxiety level and despair state of the behavior of LPS mice, indicating that the Chinese medicine formula of the present invention has the effects of anti-depression, anti-anxiety and improving mental state.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An antidepressant Chinese medicine prescription, characterized in that: The Chinese medicine formula is composed of the following raw materials in parts by weight: 4-12 parts of Bupleurum, 2-6 parts of Polygala, 2-6 parts of Ginseng, 2-8 parts of Poria, 2-8 parts of White Peony, 3-6 parts of Acorus, 4-8 parts of Atractylodes, 4-8 parts of Angelica, 3-9 parts of Gardenia, 3-9 parts of Atractylodes, 2-8 parts of Shenqu, and 2-8 parts of Licorice.

2. The antidepressant Chinese medicine prescription according to claim 1, characterized in that: The Chinese medicine formula is composed of the following raw materials in parts by weight: 6 parts of Bupleurum, 3 parts of Polygala, 3 parts of Ginseng, 6 parts of Poria, 6 parts of White Peony, 3 parts of Acorus calamus, 6 parts of Atractylodes, 6 parts of Angelica, 6 parts of Gardenia, 6 parts of Atractylodes, 6 parts of Shenqu, and 6 parts of Licorice.

3. The antidepressant Chinese medicine prescription according to claim 1 or 2, characterized in that: The Chinese medicine formula is made into decoction, pills, dripping pills, granules, tablets, hard capsules, soft capsules, decoctions, syrups, oral liquids or beverages.

4. The method for preparing the antidepressant Chinese medicine formula according to any one of claims 1 to 3, characterized in that: The raw materials are mixed, soaked and boiled according to weight, and the boiled liquid is taken to obtain a finished Chinese medicine.

5. The preparation method according to claim 4, characterized in that: Take each raw material, add 10 times the amount of water, reflux extraction twice, each time for 1 hour, combine the decoctions, filter, and concentrate under reduced pressure. The process parameters are: 0.08-0.10Mpa, rotation speed 90rpm, 60℃, to prepare the finished Chinese medicine.

6. Use of the antidepressant Chinese medicine formula according to any one of claims 1 to 3 in the preparation of antidepressant Chinese medicine products.

7. The use according to claim 6, characterized in that: The Chinese medicine product is a decoction, pill, dripping pill, granule, tablet, hard capsule, soft capsule, decoction, syrup, oral liquid or beverage.

8. The use according to claim 6, characterized in that: When used, the dosage of the Chinese medicine product is 5-20g / kg.

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