Ppv virus quantitative detection device

Abstract

The invention discloses a ppv virus quantitative detection device, which comprises a box body, a centrifugation unit, a pressurization module, a liquid discharge module, a negative pressure impurity discharge module and a controller, a centrifugation chamber and a discharge chamber are vertically arranged in the box body, and the centrifugation unit comprises a lower centrifugation disc, an upper centrifugation disc and a centrifugation driving mechanism connected with the controller. The upper centrifugal disc is rotationally mounted on the rear inner wall of the centrifugal chamber and is in transmission connection with the centrifugal driving mechanism, and a plurality of piston type centrifugal tubes are uniformly arranged on the upper centrifugal disc at intervals in the circumferential direction; the lower centrifugal disc is rotationally mounted on a bottom plate of the centrifugal chamber below the upper centrifugal disc and is in transmission connection with the centrifugal driving mechanism; the device is unique in structure and ingenious in design, and aims to improve the detection efficiency and safety, simplify the operation process, accurately control the liquid transfer amount, reduce reagent waste, reduce the detection cost and ensure the consistency and accuracy of results through an automatic and high-precision device.

Description

Technical Field

[0001] The invention belongs to the technical field of porcine parvovirus detection, and in particular relates to a PPV virus quantitative detection device. Background Art

[0002] Porcine Parvovirus (PPV) is an important swine pathogen and a member of the Parvoviridae family. It mainly infects pregnant sows, causing embryonic death, abortion, and increased mortality of newborn piglets, causing significant economic losses to the pig industry. PPV has various transmission routes, including direct contact and airborne transmission, so rapid and accurate detection and control of the virus is crucial to ensure the healthy development of the pig industry.

[0003] At present, when conducting nucleic acid detection of PPV virus, the method usually adopted is nucleic acid extraction and amplification based on PCR technology. However, when extracting nucleic acid after sample processing, traditional nucleic acid extraction requires multiple steps, including batch multiple centrifugation of the mixed solution (virus preservation solution with column binding solution added), discarding the penetration solution after centrifugation, adding universal column washing solution for elution, etc. Each step requires precise manual operation, which is easy to introduce human errors. In addition, due to the large number of pigs in large-scale farms, even if sampling and testing are carried out, each sample requires manual operation. The entire process from sample processing to obtaining purified nucleic acid for virus detection often takes several hours or even longer, which is time-consuming and affects the efficiency of virus detection. In addition, since the amount of manual liquid transfer during the operation is not easy to accurately control, it is easy to cause reagent waste and increase the detection cost. Summary of the invention

[0004] In view of the defects and problems existing in the existing porcine parvovirus detection, the present invention provides a PPV virus quantitative detection device, which has a unique structure and ingenious design. It aims to improve the detection efficiency and safety, simplify the operation process, accurately control the liquid transfer amount, reduce reagent waste, reduce detection costs, and ensure the consistency and accuracy of the results through an automated and high-precision device.

[0005] The solution adopted by the present invention to solve its technical problem is: a PPV virus quantitative detection device, including a box, a centrifugal unit, a pressurization module, a drainage module, a negative pressure impurity removal module and a controller, wherein a centrifugal chamber and a discharge chamber are vertically arranged in the box, the centrifugal unit includes a lower centrifugal disc, an upper centrifugal disc and a centrifugal drive mechanism connected to the controller, the upper centrifugal disc is rotatably mounted on the rear inner wall of the centrifugal chamber and is transmission-connected to the centrifugal drive mechanism, a plurality of piston-type centrifugal tubes are evenly spaced along the circumferential direction on the upper centrifugal disc; the lower centrifugal disc is rotatably mounted on the centrifugal disc below the upper centrifugal disc The bottom plate of the chamber is connected to the centrifugal drive mechanism in a transmission manner; the bottom plate of the centrifugal chamber below the lower centrifugal disc is provided with pure discharge holes and through discharge holes in a circumferential direction, which are connected to the discharge chamber below; the discharge chamber below the pure discharge holes and through discharge holes is provided with a pure connection pipe and a through connection pipe respectively; the lower centrifugal disc is provided with a plurality of placement grooves evenly spaced in a circumferential direction, the bottom of each placement groove is provided with a drainage groove coaxially, the bottom of the drainage groove is provided with a groove hole coaxially, and a normally open one-way drainage valve is installed, and when the centrifugal disc rotates, the groove hole will overlap and connect with the discharge hole and the impurity discharge hole in turn; each placement groove is matched with a plug-in A centrifugal adsorption tube, the centrifugal adsorption tube comprises a centrifugal outer tube, an adsorption column and a plugging assembly, the centrifugal outer tube is matched and inserted into the placement groove, and the bottom of the centrifugal outer tube extends into the drainage groove and is provided with a through hole, in which a one-way drainage valve is installed; the adsorption column is matched and installed on the top of the centrifugal outer tube, and the plugging assembly is matched and installed on the top of the adsorption column; the side wall of the centrifugal outer tube on one side of the adsorption column is provided with an air hole and a one-way air inlet valve is installed; the negative pressure impurity removal module is arranged at intervals in the centrifugal chamber on one side of the lower centrifugal disk, and is connected to the controller. When the slot hole overlaps with the drainage hole, the controller can control the negative pressure The suction end of the impurity removal module is connected to the drainage trough just above the drainage hole; the drainage module is matched and installed in the centrifugal chamber on one side of the pure drainage hole, and is connected to the controller. When the trough hole overlaps with the pure drainage hole, the controller can control the insertion end of the drainage module to dock with the sealing component of the centrifugal adsorption tube placed in the trough just above the pure drainage hole, and inject high-pressure air into the adsorption column; the boost module is connected to the domain controller, and is used to control the piston-type centrifugal tube with its bottom end facing vertically downward to dock with the sealing component of the centrifugal adsorption tube just below, so as to quantitatively discharge the liquid in the piston-type centrifugal tube into the adsorption column of the centrifugal adsorption tube through the sealing component.

[0006] The piston centrifuge tube comprises an outer tube body movably mounted on the front end surface of the upper centrifugal disk along a right-angle discharge channel, the bottom end of the outer tube body is arranged outwardly and is provided with an elastic docking component matching the blocking component, and a composite tube cover is matched and mounted on the top of the outer tube body.

[0007] The composite tube cover includes a tube cover and a piston. The tube cover is sealed and installed on the top of the outer tube body, and a socket matching the boost module is provided in the middle of the tube cover. The piston is matched and installed in the outer tube body below the tube cover, and can slide axially along the inner wall of the outer tube body to adjust the pressure of the lower chamber and control the opening and closing of the elastic docking assembly.

[0008] The boost module includes a hollow rod coaxially fixedly mounted on the outer side wall of the box body at the rear side of the upper centrifugal disk, the tail end of the hollow rod is connected to an air source, and the air source is connected to a controller; the hollow rod extends forward through the upper centrifugal disk to the front side of the centrifugal disk, and is fixed with a mounting seat, a telescopic plug matching the socket is vertically mounted on the bottom surface of the mounting seat, the telescopic plug is connected to the channel inside the hollow rod, and when the air supply channel is ventilated, the plug end of the telescopic plug automatically extends downward and is inserted into the socket.

[0009] The elastic docking assembly comprises an inner core tube, a drainage column, a drainage top spring and a docking plug matched with the blocking assembly, the inner core tube is coaxially mounted on the inner tube bottom of the outer tube body, and the internal channel of the inner core tube penetrates downwardly through the inner tube bottom, and a drainage hole connected with the internal channel is radially arranged at the bottom of one side of the inner core tube; the drainage column is axially slidably mounted in the inner core tube, and the outer annular surface of the drainage column blocks and blocks the opening in the drainage hole; the bottom end of the drainage column extends downward out of the outer tube body, and a docking plug is matched and installed, a right-angle drainage channel is matched in the drainage column, the axial port of the right-angle drainage channel penetrates downwardly through the docking plug setting, and the radial port of the right-angle drainage channel penetrates outwardly through the drainage column and matches the drainage hole setting; the top end of the drainage column extends upward out of the inner core tube, and an elastic support member a is installed, and the elastic support member a will drive the drainage column to slide upward in a natural state to separate the axial port of the right-angle drainage channel from the drainage hole, thereby blocking the drainage hole.

[0010] The sealing assembly includes a docking barrel, a sealing block and an elastic support member b. The size of the docking barrel matches the size of the docking plug and the insertion end of the drainage module; the docking barrel is coaxially installed on the inner top wall of the adsorption column, and the top of the receiving barrel is open and penetrates the inner top wall of the adsorption column upward, and the inner ring side walls on the left and right sides of the receiving barrel are respectively provided with an upper air hole and a lower liquid hole, both of which are connected to the interior of the adsorption column, and the upper air hole is located above the lower liquid hole; the sealing block is installed in the docking barrel along a vertical sliding manner through the elastic support member b, and the elastic support member b will drive the sealing block to move upward in a natural state to seal the inner orifices of the upper air hole and the lower liquid hole; the sealing block is provided with a docking channel matching the axial port of the right-angle drainage channel.

[0011] The drainage module includes a plug post coaxially arranged above the drainage hole, the plug post can be matched and inserted into the docking barrel, an air channel matching the docking channel is vertically arranged in the middle of the plug post, an air pipe is connected to the top of the air channel, the air pipe extends out of the box and is connected to an air pump, and the air pump is connected to the controller; a lifting cylinder connected to the controller is vertically installed in the centrifugal chamber on one side of the plug post, and the lifting end of the lifting cylinder is arranged upward and connected to the plug post.

[0012] A device chamber is separated in the box body at the rear side of the centrifugal chamber, and the centrifugal drive mechanism includes an upper centrifugal drive assembly and a lower centrifugal drive assembly. The fixed axis of the upper centrifugal disk extends backward into the device chamber and is transmission connected to the upper centrifugal drive assembly; the drive shaft of the lower centrifugal disk is transmission connected to the lower centrifugal drive assembly.

[0013] The outer ring surface of the centrifugal disk is evenly equipped with a plurality of docking sockets along the circumferential direction, and the docking sockets are arranged one by one corresponding to the drainage grooves and are connected; the controller can control the suction end of the negative pressure impurity removal module to extend and dock with the docking sockets.

[0014] Beneficial effects of the present invention: The PPV virus quantitative detection device provided by the present invention has the following beneficial effects: The PPV virus quantitative detection device provided by the present invention integrates multiple modules such as centrifugation, pressurization, drainage and negative pressure impurity removal, and is managed uniformly by a controller, thereby realizing full process automation from sample processing to nucleic acid extraction. This not only reduces the need for manual operation, but also improves the consistency and repeatability of the experiment.

[0015] The PPV virus quantitative detection device provided by the present invention adopts a double-layer centrifugal disk design, which can process multiple samples at the same time, greatly increasing the processing volume per unit time. In addition, the piston centrifugal tube can accurately control the liquid transfer, ensuring that the amount of liquid in each step is accurate.

[0016] The PPV virus quantitative detection device provided by the present invention effectively removes impurities through a negative pressure impurity removal module and specifically captures the target substance using a specific adsorption material, thereby ensuring the high purity of the final product. At the same time, the optimized elution step helps to improve the recovery rate of the target substance.

[0017] The PPV virus quantitative detection device provided by the present invention reserves storage space for various liquids such as column washing liquid, eluent, etc., and can flexibly adjust the scheme according to different experimental requirements, so as to be suitable for various types of sample analysis.

[0018] The automated process of the PPV virus quantitative detection device provided by the present invention reduces the experimental preparation time and manpower investment; and the efficient processing capacity and higher yield mean less reagent consumption, thereby reducing the overall operating cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0020] Figure 2 It is a schematic diagram of the structure of the centrifugal unit of the present invention.

[0021] Figure 3 It is a structural schematic diagram of the centrifugal drive assembly of the present invention.

[0022] Figure 4 It is a schematic diagram of the structure of the negative pressure impurity removal module of the present invention.

[0023] Figure 5 It is a schematic diagram of the structure of a piston type centrifugal tube and a centrifugal adsorption tube of the present invention.

[0024] Figure 6 It is a schematic diagram of the matching relationship between the piston type centrifuge tube and the centrifugal adsorption tube of the present invention.

[0025] Figure 7 It is a schematic diagram of the structure of the boost module of the present invention.

[0026] Figure 8 It is a schematic diagram of the structure of the liquid discharge module of the present invention.

[0027] Fig. 9 It is a schematic diagram of the structure of the liquid injection module of the present invention.

[0028] Fig.10 It is a schematic diagram of the matching relationship between the liquid injection module and the piston type centrifuge tube of the present invention.

[0029] In the figure, the reference numerals are: 1 is a box body, 11 is a horizontal partition, 12 is a device cavity, 13 is a vertical partition, 2 is a centrifugal unit, 21 is an upper centrifugal disc, 211 is a pipe sleeve, 22 is a lower centrifugal disc, 222 is a placement slot, 223 is a docking socket, 224 is a drainage slot, 225 is a normally open one-way drainage valve, 23 is a motor seat, 25 is a worm, 24 is a turbine, 26 is a drive motor, 3 is a booster module, 31 is a hollow rod, 32 is a mounting seat, 33 is a telescopic plug, 331 is a plug shell, 332 is a plug body, 333 is a piston block, 334 is a retraction top spring, 4 is a piston type centrifugal tube, 41 is an outer tube body, 42 is a composite tube cover, 421 is a tube cover, 422 is a piston, 43 is an inner core tube, 431 is an injection hole, 432 is a drainage hole, 44 is a drainage column, 441 is a right-angle liquid channel, 45 is a docking plug, 46 is an injection top spring, and 47 is a drainage top spring , 5 is a centrifugal adsorption tube, 51 is a centrifugal outer tube, 52 is an adsorption column, 53 is a blocking block, 531 is a docking channel, 55 is an elastic support member b, 551 is a guide column, 552 is a support spring, 553 is a limit plate, 54 is a docking barrel, 541 is a lower liquid hole, 542 is an upper air hole, 6 is a liquid discharge module, 61 is a lifting cylinder, 62 is a connecting arm, 63 is a plug column, 631 is an airway, 64 is an air pipe, 7 is a negative pressure impurity removal module, 71 is a boss, 72 is a telescopic rod a, 73 is a plug seat a, 74 is a docking plug a, 75 is a negative pressure tube, 8 is a liquid injection module, 81 is a platform plate, 82 is a telescopic cylinder b, 83 is a slide seat a, 84 is a plug seat b, 85 is a docking plug b, and 86 is a liquid supply pipe. DETAILED DESCRIPTION

[0030] The present invention is further described below in conjunction with the accompanying drawings and embodiments. Example

[0031] In view of the problems raised in the above background technology, the present invention provides a PPV virus quantitative detection device, such as Figure 1-8 The figure shows a box body 1, a centrifugal unit 2, a booster module 3, a drainage module 6, a negative pressure impurity removal module 7, a piston 422 type centrifugal tube 4, a centrifugal adsorption tube 5 and a controller. A centrifugal chamber and a discharge chamber are vertically arranged in the box body 1. There are many ways to set the centrifugal chamber and the discharge chamber. For example, a horizontal partition 11 is matched in the box body 1, and the horizontal partition 11 divides the internal space of the box body 1 from top to bottom into a centrifugal chamber and a discharge chamber.

[0032] The centrifugal unit 2 includes a lower centrifugal disc 22, an upper centrifugal disc 21 and a centrifugal drive mechanism connected to the controller. The upper centrifugal disc 21 is rotatably mounted on the rear inner wall of the centrifugal chamber and is in transmission connection with the centrifugal drive mechanism. The lower centrifugal disc 22 is rotatably mounted on the bottom plate of the centrifugal chamber below the upper centrifugal disc 21 and is in transmission connection with the centrifugal drive mechanism. The controller can control the rotation of the lower centrifugal disc 22 and the upper centrifugal disc 21 through the centrifugal drive mechanism. Specifically: like Figure 1 and Figure 3 As shown, a device chamber is separated by a vertical partition 13 in the box body 1 at the rear side of the centrifugal chamber, and an upper centrifugal disk 21 is rotatably mounted on the vertical partition 13. The centrifugal drive mechanism includes an upper centrifugal drive assembly and a lower centrifugal drive assembly. The fixed axis of the upper centrifugal disk 21 extends backward into the device chamber and is transmission-connected to the upper centrifugal drive assembly. The upper centrifugal drive assembly includes a drive motor 26, a worm 25 and a turbine 24. The turbine 24 is fixedly sleeved on the fixed axis of the upper centrifugal disk 21 in the device chamber. The worm 25 is rotatably mounted on the vertical partition 13 above the turbine 24 and meshes with the turbine 24. A motor seat 23 is matched on the vertical partition 13 on one side of the worm 25. The drive motor 26 is fixedly mounted on the motor seat 23 and is control-connected to the controller. The motor shaft of the drive motor 26 is arranged toward the end of the worm 25 and is transmission-connected together through a coupling.

[0033] The drive shaft of the lower centrifugal disc 22 is transmission-connected to the lower centrifugal drive assembly. There are many types of lower centrifugal drive assemblies. In this embodiment, the lower centrifugal drive assembly is installed in the discharge chamber, and includes a horizontal centrifugal motor and a transmission gear set. The drive shaft of the lower centrifugal disc 22 extends downward into the discharge chamber and is transmission-connected to the horizontal centrifugal motor through the transmission gear set. The horizontal centrifugal motor is connected to the controller.

[0034] A plurality of piston 422 type centrifuge tubes 4 are evenly spaced along the circumferential direction on the upper centrifugal disk 21. In this embodiment, there are two piston 422 type centrifuge tubes 4 on the upper centrifugal disk 21 to store column washing liquid and eluent respectively; a pure discharge hole and a through discharge hole connected to the discharge chamber below are spaced along the circumferential direction on the bottom plate of the centrifugal chamber below the lower centrifugal disk 22, and a pure connection pipe and a through connection pipe are respectively arranged in the discharge chamber below the pure discharge hole and the through discharge hole; a plurality of placement grooves 222 for inserting centrifugal adsorption tubes 5 are evenly spaced along the circumferential direction on the lower centrifugal disk 22, and a drainage groove 224 is provided at the bottom of each placement groove 222, and the drainage groove 224 is straight The diameter size is smaller than the diameter size of the placement groove 222. The bottom of the drainage groove 224 is provided with a slot hole, and a normally open one-way drainage valve 225 is installed. When the centrifugal disk rotates, the slot hole will overlap and connect with the discharge hole and the impurity discharge hole in sequence; there are many types of normally open one-way drainage valves 225. The normally open one-way drainage valve 225 in this embodiment adopts a diaphragm check valve. In the absence of external pressure difference, the diaphragm of the diaphragm check valve remains in a relaxed state, and the liquid flowing into the drainage groove 224 can flow out through the slot hole. When negative pressure is generated in the drainage groove 224, the external atmospheric pressure will cause the diaphragm to bulge upward, seal the outlet, and close the slot hole.

[0035] Each placement slot 222 is matched with a centrifugal adsorption tube 5. When in use, the number of installed centrifugal adsorption tubes 5 can be selected according to the number of nucleic acid individuals to be extracted. The centrifugal adsorption tube 5 includes a centrifugal outer tube 51, an adsorption column 52 and a plugging component. The centrifugal outer tube 51 is matched and inserted in the placement slot 222, and the bottom of the centrifugal outer tube 51 extends into the drainage slot 224 and is provided with a through hole, in which a one-way drainage valve is installed; the adsorption column 52 is matched and installed on the top of the centrifugal outer tube 51, and the plugging component is matched and installed on the adsorption column 52. The top of the column 52; the side wall of the centrifugal outer tube 51 on one side of the adsorption column 52 is provided with an air hole, and a one-way air inlet valve is installed; the negative pressure impurity removal module 7 is arranged in the centrifugal chamber on one side of the lower centrifugal disk 22 at intervals, and is connected to the controller. When the slot hole overlaps with the drainage hole, the controller can control the suction end of the negative pressure impurity removal module 7 to communicate with the drainage groove 224 just above the drainage hole, and extract the air in the drainage groove 224 to drive the normally open one-way drainage valve 225 to close and extract the air in the centrifugal outer tube 51 inserted in the placement groove 222. Specifically: like Figure 5As shown, a plurality of docking sockets 223 are evenly installed on the outer ring surface of the centrifugal disk along the circumferential direction, and the docking sockets 223 are arranged one by one with the drainage grooves 224 and are connected; the controller can control the suction end of the negative pressure impurity removal module 7 to extend and dock with the docking socket, and the negative pressure impurity removal module 7 includes a fixedly installed centrifugal chamber bottom boss 71, and a slide seat is radially slidably installed on the boss 71 at the center of the lower centrifugal disk 22, and a telescopic device connected to the controller is matched and installed on the boss 71 on the side of the slide away from the lower centrifugal disk 22. Rod a72, telescopic rod a72 is an electric push rod or a pneumatic push rod, telescopic rod a72 is connected to the slide, and the controller can drive the slide to move through the telescopic rod a72; a plug seat a73 is matched and installed on the slide, and a section of the plug seat a73 facing the lower centrifugal disk 22 is matched and installed with a docking plug a74, and the tail end of the docking plug a74 is matched and connected with a negative pressure pipe 75, and the other end of the negative pressure pipe 75 extends out of the box body 1 and is connected to a negative pressure pump, which is connected to the controller, and the lower centrifugal disk 22 is connected to the lower centrifugal disk 22. After the mixed liquid in the centrifugal adsorption tube 5 is centrifuged, the controller will control the centrifugal adsorption tube 5 to move to the top of the drainage port, so that the slot below the centrifugal adsorption tube 5 is connected to the drainage hole. When the controller controls the telescopic rod a72 to push the slide seat to drive the plug seat a73 to move to the lower centrifugal disk 22, the docking plug a74 will match and be inserted into the docking socket 223 corresponding to the centrifugal adsorption tube 5. When the negative pressure pump is working, the normally open one-way drainage valve 225 in the slot will be closed. Under the action of negative pressure, the one-way drain valve at the bottom of the centrifugal adsorption tube 5 will open, thereby sucking and discharging the penetrating liquid in the centrifugal adsorption tube 5, and during the process of washing and suctioning the penetrating liquid, the one-way air inlet valve on the centrifugal adsorption tube 5 will open, thereby replenishing air into the centrifugal adsorption tube 5 to prevent the negative pressure from acting on the adsorption column 52, and the penetrating liquid discharged into the drainage groove 224 will be discharged into the pure pipe through the drainage hole through the opened normally open one-way drain valve 225 when the docking tube a74 is separated from the plug socket a73.

[0036] The boost module 3 is connected to the controller, and is used to control the piston 422 type centrifuge tube 4 with the bottom end facing vertically downward to dock with the sealing component of the centrifugal adsorption tube 5 directly below, so as to quantitatively discharge the liquid in the piston 422 type centrifuge tube 4 into the adsorption column 52 of the centrifugal adsorption tube 5 through the sealing component; the piston 422 type centrifuge tube 4 includes an outer tube body 41 movably installed radially on the front end surface of the upper centrifugal disk 21, and there are multiple ways to install the outer tube body 41, for example: the front end surface of the upper centrifugal disk 21 is evenly spaced along the circumferential direction. A plurality of pipe sleeves 211 for inserting the outer tube body 41 are installed, and the pipe sleeves 211 are arranged radially along the upper centrifugal disk 21, and a clamp is matched and installed on the pipe sleeves 211 for fixing the outer tube body 41. The bottom end of the outer tube body 41 is arranged outward, and is provided with an elastic docking assembly matching the plugging assembly. A composite tube cover 42 is matched and installed on the top of the outer tube body 41. When the piston 422 type centrifuge tube 4 rotates vertically downward, the controller can control the air supply end of the booster module 3 to extend downward and dock with the composite tube cover 42 on the top of the piston 422 type centrifuge tube 4, drive the elastic docking assembly to extend downward and dock with the plugging assembly of the centrifugal adsorption tube 5 directly below, and quantitatively discharge the liquid in the piston 422 type centrifuge tube 4 into the adsorption column 52 of the centrifugal adsorption tube 5, specifically: like Figure 6 As shown, the composite tube cover 42 includes a tube cover 421 and a piston 422. The tube cover 421 is sealed and installed on the top of the outer tube body 41, and a socket matching the boost module 3 is provided in the middle of the tube cover 421. The piston 422 is matched and installed in the outer tube body 41 below the tube cover 421, and can slide axially along the inner wall of the outer tube body 41 to adjust the pressure in the lower chamber, thereby controlling and driving the opening and closing of the elastic docking assembly.

[0037] The elastic docking assembly includes an inner core tube 43, a drainage column 44, a drainage top spring 47 and a docking plug 45 that matches the blocking assembly. The inner core tube 43 is coaxially installed on the inner tube bottom of the outer tube body 41, and the internal channel of the inner core tube 43 passes through the inner tube bottom downward. A drainage hole 432 connected to the internal channel is radially arranged at the bottom of one side of the inner core tube 43; the drainage column 44 is axially slidably installed in the inner core tube 43, and the outer annular surface of the drainage column 44 blocks and blocks the inner opening of the drainage hole 432; the bottom end of the drainage column 44 extends downward out of the outer tube body 41, and is matched with the docking plug 45. A right-angle drainage channel is matched in the drainage column 44. The axial port passes through the docking plug 45 downward, and the radial port of the right-angle drainage channel passes through the drainage column 44 outward and is matched with the drainage hole 432; the top of the drainage column 44 extends upward from the inner core tube 43 and is installed with an elastic support member a, which includes a drainage top spring 47. The top of the drainage column 44 is matched with a ring plate with a diameter larger than the diameter of the inner core tube 43. The drainage top spring 47 is matched and installed on the drainage column 44 between the ring plate and the inner core tube 43. In a natural state, the drainage top spring 47 will drive the drainage column 44 to slide upward to separate the axial port of the right-angle drainage channel from the drainage hole 432, thereby sealing the drainage hole 432.

[0038] The boost module 3 includes a hollow rod 31 coaxially fixedly mounted on the outer wall of the box body 1 at the rear side of the upper centrifugal disk 21, the tail end of the hollow rod 31 is connected to an air source, and the air source is connected to a controller; the center of the upper centrifugal disk 21 is axially provided with an avoidance hole that penetrates the disk body and the fixed axis of the upper centrifugal disk 21, the front end of the hollow rod 31 passes through the avoidance hole and extends to the enclosed area of ​​the piston 422-type centrifugal tube 4, and is fixed with a mounting seat 32, and a telescopic plug 33 matching the socket is vertically mounted on the bottom surface of the mounting seat 32, the telescopic plug 33 is connected to the channel inside the hollow rod 31, and when the air supply channel is ventilated, the plug end of the telescopic plug 33 automatically extends downward and is inserted into the socket; the telescopic plug 33 includes a plug shell 331 vertically mounted on the bottom surface of the mounting seat 32, a piston block 333 is vertically slidably mounted in the plug shell 331, a plug body 332 matching the socket is vertically mounted on the bottom surface of the piston block 333, and the plug body 3 The bottom end of 32 extends downwardly out of the plug shell 331, and the middle part of the piston block 333 is axially provided with an air hole connected to the internal channel of the hollow rod 31, and the air hole penetrates the plug body 332 downward, and a one-way exhaust valve is matched and installed in the air hole of the plug body 332, and a retraction top spring 334 is matched and installed on the plug body 332 in the plug shell 331 below the piston block 333. The retraction top spring 334 will push the piston block 333 upward to drive the plug body 332 to retract into the plug shell 331 when the internal channel of the hollow rod 31 is not ventilated. When the controller controls the air source to inject air into the hollow rod 31, since the one-way exhaust valve is matched and installed in the air hole of the plug body 332, the air pressure will drive the plug body 332 to drive the piston 422 to move downward and extend quickly. When the plug body 332 extends downward to the limit state, as the air pressure in the hollow rod 31 increases, the one-way exhaust valve will automatically open.

[0039] The plugging assembly includes a docking barrel 54, a plugging block 53 and an elastic support member b55. The size of the docking barrel 54 matches the size of the docking plug 45 and the insertion end of the drainage module 6. The docking barrel 54 is coaxially installed on the inner top wall of the adsorption column 52, and the top of the receiving barrel is open and penetrates the inner top wall of the adsorption column 52 upward. The inner ring side walls on the left and right sides of the receiving barrel are respectively provided with an upper air hole 542 and a lower liquid hole 541. The upper air hole 542 and the lower liquid hole 541 are both connected to the inside of the adsorption column 52, and the upper air hole 542 is located above the lower liquid hole 541. The plugging block 53 is vertically slidably installed in the docking barrel 54 through the elastic support member b55. The elastic support member b55 includes a guide column 551 vertically installed on the bottom surface of the plugging block 53. The guide column 551 passes downward through the bottom of the docking barrel 54 and extends into the adsorption column 52, and is connected to the bottom of the docking barrel 54. A limit plate 553 is connected, and a supporting spring 552 is matched on the guide column 551 in the docking barrel 54. The supporting spring 552 will drive the blocking block 53 to move upward in a natural state so that the limit plate 553 contacts the bottom of the docking barrel 54, and the inner openings of the upper air hole 542 and the lower liquid hole 541 are blocked by the outer annular surface of the blocking block 53; a docking channel 531 matching the axial port of the right-angle discharge channel is provided in the blocking block 53, and when the docking plug 45 pushes the blocking block 53 downward to insert the radial port of the right-angle discharge channel in the docking barrel 54 and communicate with the discharge hole 432, the axial port of the right-angle discharge channel is communicated with the lower liquid hole 541 through the docking channel 531. In this state, the upper air hole 542 is connected to the outside through the annular space between the discharge column 44 and the inner wall of the docking barrel 54; specifically: When the controller drives the centrifugal adsorption tube 5 that needs to be injected with liquid on the lower centrifugal disk 22 to rotate and move to the bottom of the telescopic plug 33 through the centrifugal driving device, the controller will drive the piston 422 type centrifugal tube 4 storing the corresponding liquid on the upper centrifugal disk 21 to rotate to the bottom of the telescopic plug 33 through the centrifugal driving device according to the injection demand, and then the controller controls the boost module 3 to start injecting air into the outer tube body 41 above the piston 422 through the jack on the top of the composite tube cover 42, pushing the piston 422 downward, driving the docking plug 45 at the bottom end of the drainage column 44 to slide downward and extend the pushing and blocking block 53 to be inserted into the docking barrel 54. As the piston 422 moves downward, when the drainage column 44 extends to the limit state, the right-angle drainage channel in the drainage column 44 will be connected to the drainage hole 432 and the lower liquid hole 541 at the same time, so that the downward movement of the piston 422 can be controlled according to demand to stably discharge the liquid in the piston 422 type centrifugal tube 4 into the adsorption column 52 of the centrifugal adsorption tube 5.

[0040] The drainage module 6 includes a plug post 63 coaxially arranged above the drainage hole, the plug post 63 can be matched and inserted into the docking barrel 54, and an air channel 631 matching the docking channel 531 is vertically arranged in the middle of the plug post 63, and an air pipe 64 is connected to the top of the air channel 631, and the air pipe 64 extends out of the box body 1 and is connected to an air pump, and the air pump is connected to the controller; a lifting cylinder 61 connected to the controller is vertically installed in the centrifugal chamber on one side of the plug post 63, and the lifting end of the lifting cylinder 61 is arranged upward and connected to the plug post 63. The lifting end of the lifting cylinder 61 is connected to the plug post 63 in many ways, for example, the lifting end of the lifting cylinder 61 is connected to the connecting arm 62, and the other end of the connecting arm 62 is fixedly sleeved on the top of the plug post 63. The controller can drive the plug post 63 to push the blocking block 53 downward and insert it into the docking barrel 54 through the lifting cylinder 61, so that the air channel 631 is connected to the lower liquid hole 541 through the docking channel 531. In this state, the upper air The hole 542 is blocked and blocked by the outer annular surface of the plug 63. Therefore, when the centrifugal purification of the nucleic acid injected with the elution solution is completed, the controller first controls the centrifugal adsorption tube 5 to move to the top of the drainage port, and after controlling the negative pressure impurity removal module 7 to pump the permeated liquid in the centrifugal outer tube 51 into the pure receiving tube, the controller will control the lower centrifugal disk 22 to rotate so that the centrifugal adsorption tube 5 after drainage moves to the top of the pure drainage hole, and control the bottom slot hole of the placement slot 222 for installing the centrifugal adsorption tube 5 to overlap and connect with the pure drainage hole. Then the controller will control the drainage module 6 to start, drive the lifting cylinder 61 to drive the plug 63 to push the blocking block 53 downward and insert it into the docking barrel 54, so that the airway 631 is connected with the lower liquid hole 541 through the docking channel 531, and then start the air pump to pump high-pressure gas into the adsorption column 52, and discharge the purified nucleic acid through the bottom hole of the adsorption column 52 through the centrifugal outer tube 51 drainage slot 224 and the pure drainage hole into the pure receiving tube for detection.

[0041] When in use, the PPV virus quantitative detection device provided in this embodiment uses a piston 422-type centrifuge tube 4 to store a mixed liquid. After the piston 422-type centrifuge tube 4 storing the mixed liquid and the additive liquid is installed on the upper centrifugal disk 21, the nucleic acid extraction device operating parameters of the controller are adjusted according to the installation position number of the piston 422-type centrifuge tube 4, so that the controller injects the mixed liquid into the same centrifugal adsorption tube 5 in batches through the boosting module 3 for centrifugal treatment according to the device operating parameters, and automatically and quantitatively adds column washing liquid, elution liquid, etc. to the centrifugal adsorption tube 5 according to the extraction parameters, and uses the controller to control each module to realize the full process automation of nucleic acid extraction and reduce the need for manual operation. Example

[0042] The difference between Example 2 and Example 1 is that Figure 1 As shown, a liquid injection module 8 connected to a controller is provided in the centrifugal chamber outside the upper centrifugal disc 21 along the circumferential direction. Figure 5 and Figure 6The drainage column 44 between the docking plug 45 and the outer tube body 41 is matched with an injection top spring 46, and the inner core tube 43 above the drainage hole 432 is matched with an injection hole 431 connected to the inner core tube 43. The injection top spring 46 and the drainage top spring 47 will drive the drainage column 44 to maintain a stable state in a natural state, so that the radial port of the right-angle drainage channel is located between the injection hole 431 and the drainage hole 432. When the piston 422 type centrifuge tube 4 follows the upper centrifugal disk 21 to rotate the tail end toward the injection module 8, the controller can control the injection end of the injection module 8 to extend and match and insert into the right-angle drainage channel on the docking plug 45, and push the top The docking plug 45 drives the drainage column 44 to retract into the outer tube body 41, so that the radial port of the right-angle drainage channel overlaps and is connected with the injection hole 431, thereby being able to automatically inject the additive liquid into the piston 422 type centrifuge tube 4, saving the time for nucleic acid extraction. For example: during the rotation of the lower centrifugal disk 22 for centrifugal operation, the column washing liquid is directly injected into the piston 422 type centrifuge tube 4 with only the last transfer amount of the mixed liquid remaining through the cooperation of the injection module 8 and the piston 422 type centrifuge tube 4. When the next drainage is performed, the mixed liquid with the oil column washing liquid can be directly discharged into the corresponding centrifugal adsorption tube 5 for direct centrifugation, thereby saving placement time.

[0043] There are many types of injection modules 8, for example: Fig. 9 and Fig.10 As shown, the injection module 8 includes a platform plate 81, on which a telescopic cylinder b82 is radially installed along the upper centrifugal disk 21, the telescopic cylinder b82 is connected to the controller, a slide seat a83 is matched and installed on the telescopic end of the telescopic rod b, a plug seat b84 is matched and installed on the slide seat a83, and a docking plug b85 is matched and installed on the end of the plug seat facing the upper centrifugal disk 21, and the docking plug b85 passes through the plug seat b84 backward at the end away from the upper centrifugal disk 21, and is connected to a liquid supply pipe 86, and the liquid supply pipe 86 is connected to a drainage mechanism, and the drainage mechanism is connected to the controller. When the controller controls the telescopic cylinder b82 to extend, the docking plug b85 will be matched and inserted into the right-angle drainage channel on the docking plug 45, and when the docking plug b85 at the front end of the plug seat b84 is fully inserted into the right-angle drainage channel on the docking plug 45, as the telescopic cylinder b82 continues to extend, the plug seat b84 will push the docking plug 45 to drive the drainage column 44 to retract into the outer tube body 41.

[0044] It should be understood that the above specific embodiments of the present invention are only used to illustrate or explain the principles of the present invention, and do not constitute a limitation of the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc. made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. In addition, the appended claims of the present invention are intended to cover all changes and modifications that fall within the scope and boundaries of the appended claims, or the equivalent forms of such scope and boundaries.

Claims

1. A PPV virus quantitative detection device, characterized in that: The invention comprises a casing, a centrifugal unit, a pressurizing module, a drainage module, a negative pressure impurity removal module and a controller, wherein a centrifugal chamber and a discharge chamber are vertically arranged in the casing, wherein the centrifugal unit comprises a lower centrifugal disc, an upper centrifugal disc and a centrifugal drive mechanism connected to the controller, wherein the upper centrifugal disc is rotatably mounted on the rear inner wall of the centrifugal chamber and is transmission-connected to the centrifugal drive mechanism, wherein a plurality of piston-type centrifugal tubes are evenly spaced along the circumferential direction on the upper centrifugal disc; wherein the lower centrifugal disc is rotatably mounted on the bottom plate of the centrifugal chamber below the upper centrifugal disc and is transmission-connected to the centrifugal drive mechanism; wherein the lower centrifugal disc is rotatably mounted on the bottom plate of the centrifugal chamber below the upper centrifugal disc and is transmission-connected to the centrifugal drive mechanism; wherein the lower centrifugal disc is rotatably mounted on the rear inner wall of the centrifugal chamber ... The bottom plate of the centrifugal chamber below the disc is provided with pure drainage holes and through drainage holes in the circumferential direction, which are connected to the discharge chamber below. The discharge chamber below the pure drainage holes and through drainage holes is provided with a pure connection pipe and a through connection pipe respectively. The lower centrifugal disc is provided with multiple placement grooves in the circumferential direction at even intervals. The bottom of each placement groove is provided with a drainage groove coaxially. The bottom of the drainage groove is provided with a groove hole coaxially, and an open one-way drainage valve is installed. When the centrifugal disc rotates, the groove hole will overlap and connect with the discharge hole and the impurity discharge hole in turn. Each placement groove is matched with a centrifugal adsorption tube, and the centrifugal adsorption tube includes a centrifugal outer The centrifugal outer tube is matched and inserted in the placement groove, and the bottom of the centrifugal outer tube extends into the drainage groove and is provided with a through hole, and a one-way drainage valve is installed in the through hole; the adsorption column is matched and installed on the top of the centrifugal outer tube, and the sealing assembly is matched and installed on the top of the adsorption column; the side wall of the centrifugal outer tube on one side of the adsorption column is provided with an air hole and a one-way air inlet valve is installed; the negative pressure impurity removal module is arranged at intervals in the centrifugal chamber on one side of the lower centrifugal disk, and is connected to the controller. When the slot hole overlaps with the drainage hole, the controller can control the suction end of the negative pressure impurity removal module. It is connected to the drainage trough just above the drainage hole; the drainage module is matched and installed in the centrifugal chamber on one side of the pure drainage hole, and is connected to the controller. When the trough hole overlaps with the pure drainage hole, the controller can control the insertion end of the drainage module to dock with the sealing component of the centrifugal adsorption tube placed in the trough just above the pure drainage hole, and inject high-pressure air into the adsorption column; the boost module is connected to the domain controller, and is used to control the piston-type centrifugal tube with its bottom end facing vertically downward to dock with the sealing component of the centrifugal adsorption tube just below, so as to quantitatively discharge the liquid in the piston-type centrifugal tube into the adsorption column of the centrifugal adsorption tube through the sealing component.

2. The PPV virus quantitative detection device according to claim 1, characterized in that: The piston centrifugal tube comprises an outer tube body movably mounted radially on the front end surface of the upper centrifugal disk, the bottom end of the outer tube body is arranged outwardly and is provided with an elastic docking component matching the blocking component, and a composite tube cover is matched and mounted on the top of the outer tube body.

3. The PPV virus quantitative detection device according to claim 2, characterized in that: The composite tube cover includes a tube cover and a piston. The tube cover is sealed and installed on the top of the outer tube body, and a socket matching the boost module is provided in the middle of the tube cover. The piston is matched and installed in the outer tube body below the tube cover, and can slide axially along the inner wall of the outer tube body to adjust the pressure of the lower chamber and control the opening and closing of the elastic docking assembly.

4. The PPV virus quantitative detection device according to claim 3, characterized in that: The boost module includes a hollow rod coaxially fixedly mounted on the outer side wall of the box body at the rear side of the upper centrifugal disk, the tail end of the hollow rod is connected to an air source, and the air source is connected to a controller; the hollow rod extends forward through the upper centrifugal disk to the front side of the centrifugal disk, and is fixed with a mounting seat, a telescopic plug matching the socket is vertically mounted on the bottom surface of the mounting seat, the telescopic plug is connected to the channel inside the hollow rod, and when the air supply channel is ventilated, the plug end of the telescopic plug automatically extends downward and is inserted into the socket.

5. The PPV virus quantitative detection device according to claim 3, characterized in that: The elastic docking assembly comprises an inner core tube, a drainage column, a drainage top spring and a docking plug matched with the blocking assembly, the inner core tube is coaxially mounted on the inner tube bottom of the outer tube body, and the internal channel of the inner core tube penetrates downwardly through the inner tube bottom, and a drainage hole connected with the internal channel is radially arranged at the bottom of one side of the inner core tube; the drainage column is axially slidably mounted in the inner core tube, and the outer annular surface of the drainage column blocks and blocks the opening in the drainage hole; the bottom end of the drainage column extends downward out of the outer tube body, and a docking plug is matched and installed, a right-angle drainage channel is matched in the drainage column, the axial port of the right-angle drainage channel penetrates downwardly through the docking plug setting, and the radial port of the right-angle drainage channel penetrates outwardly through the drainage column and matches the drainage hole setting; the top end of the drainage column extends upward out of the inner core tube, and an elastic support member a is installed, and the elastic support member a will drive the drainage column to slide upward in a natural state to separate the axial port of the right-angle drainage channel from the drainage hole, thereby blocking the drainage hole.

6. The PPV virus quantitative detection device according to claim 5, characterized in that: The sealing assembly includes a docking barrel, a sealing block and an elastic support member b. The size of the docking barrel matches the size of the docking plug and the insertion end of the drainage module; the docking barrel is coaxially installed on the inner top wall of the adsorption column, and the top of the receiving barrel is open and penetrates the inner top wall of the adsorption column upward, and the inner ring side walls on the left and right sides of the receiving barrel are respectively provided with an upper air hole and a lower liquid hole, both of which are connected to the interior of the adsorption column, and the upper air hole is located above the lower liquid hole; the sealing block is installed in the docking barrel along a vertical sliding manner through the elastic support member b, and the elastic support member b will drive the sealing block to move upward in a natural state to seal the inner orifices of the upper air hole and the lower liquid hole; the sealing block is provided with a docking channel matching the axial port of the right-angle drainage channel.

7. The PPV virus quantitative detection device according to claim 6, characterized in that: The drainage module includes a plug post coaxially arranged above the drainage hole, the plug post can be matched and inserted into the docking barrel, an air channel matching the docking channel is vertically arranged in the middle of the plug post, an air pipe is connected to the top of the air channel, the air pipe extends out of the box and is connected to an air pump, and the air pump is connected to the controller; a lifting cylinder connected to the controller is vertically installed in the centrifugal chamber on one side of the plug post, and the lifting end of the lifting cylinder is arranged upward and connected to the plug post.

8. The PPV virus quantitative detection device according to claim 1, characterized in that: A device chamber is separated in the box body at the rear side of the centrifugal chamber, and the centrifugal drive mechanism includes an upper centrifugal drive assembly and a lower centrifugal drive assembly. The fixed axis of the upper centrifugal disk extends backward into the device chamber and is transmission connected to the upper centrifugal drive assembly; the drive shaft of the lower centrifugal disk is transmission connected to the lower centrifugal drive assembly.

9. The PPV virus quantitative detection device according to claim 1, characterized in that: The outer ring surface of the centrifugal disk is evenly equipped with a plurality of docking sockets along the circumferential direction, and the docking sockets are arranged one by one corresponding to the drainage grooves and are connected; the controller can control the suction end of the negative pressure impurity removal module to extend and dock with the docking sockets.

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