Indoor seedling raising method for mangrove plant acanthus ilicifolius

Abstract

The invention discloses an indoor seedling raising method for mangrove plant acanthus ilicifolius. The indoor seedling raising method comprises the steps of seed pretreatment, seed germination and seedling indoor cultivation. The method comprises the following specific steps: putting pretreated acanthus ilicifolius seeds into a seedling raising tray filled with sand, and germinating by controlling temperature, salinity and nutrition conditions. Placing the germinated robust seedlings on a cultivation module, and harvesting acanthus ilicifolius seedlings after the seedlings are cultivated to the required height. According to the acanthus ilicifolius seedling cultivation method, indoor vertical cultivation facilities are utilized, specific light intensity, light period, temperature, salinity and the application amount of organic fertilizer, calcium, magnesium and microelements are set, the limitation on conditions such as soil fertility, climate, sunlight and salinity is eliminated, and rapid and high-density cultivation of acanthus ilicifolius seedlings can be achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and particularly relates to an indoor seedling raising method for a mangrove plant Acanthus spheniscus. Background Art

[0002] Mangroves are woody plant communities that grow in the intertidal zones of tropical and subtropical coasts or at river estuaries. They are coastal habitats of great ecological significance. They play an irreplaceable role compared to terrestrial forests in terms of wave dissipation and bank protection, coastal landscape optimization, seawater purification, maintaining offshore biodiversity, carbon fixation and storage, scientific research and ecological experience. However, in the past half century, driven by economic interests, coastal development, drainage, reclamation, aquaculture, and environmental pollution in mangrove areas have continued to emerge. Due to the strong interference of human activities, the area of ​​mangroves has sharply decreased worldwide. Therefore, it is urgent to carry out mangrove afforestation.

[0003] Acanthus Acanthus ilicifolius ) is an upright shrub of the Acanthaceae family and the genus Acanthus, up to 2 meters high. It grows on the southern coast of my country and in coastal areas where tides can reach, and is an important component of mangroves. Acanthus is mainly propagated through mangrove beach nurseries and light beach nurseries. However, nursery seedling cultivation is not only limited by soil, water sources, temperature, salinity and other conditions, but also has the defects of low seedling success rate and slow seedling cultivation speed. Therefore, shortening the seedling cultivation time of Acanthus and increasing the seedling success rate are the keys to restoring mangroves.

[0004] Compared with the outdoor nursery planting method, the use of indoor vertical cultivation facilities for seedling cultivation places the seedling cultivation process in an artificially regulated growth environment and nutrient supply, which has the characteristics of stable growth environment, sufficient nutrient supply and high space utilization, thereby greatly shortening the seedling cultivation cycle, significantly improving the seedling growth rate, and realizing the rapid and high-density cultivation of Acanthus seedlings. Summary of the invention

[0005] The purpose of the present invention is to provide an indoor seedling cultivation method for the mangrove plant Acanthus truncatus. By using indoor vertical cultivation facilities, specific light intensity, photoperiod, temperature, salinity, and application amounts of organic fertilizer, calcium, magnesium and trace elements are set, thereby getting rid of the restrictions on soil fertility, climate sunshine and salinity and other conditions, and realizing rapid and high-density cultivation of Acanthus truncatus seedlings.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: A method for indoor seedling cultivation of a mangrove plant Acanthus sphaerocephalus comprises the following steps: (1) Seed pretreatment: Sterilize the Acanthus truncatus seeds with a carbendazim solution for 15 min to obtain pretreated Acanthus truncatus seeds; (2) Seed germination: insert one quarter of the pre-treated Acanthus truncatus seeds into the holes of the seedling tray. The culture medium in the holes is sand. Spray with clean water and place under light. Adjust the environmental temperature and relative humidity for seed germination, add trace elements, and wait for the Acanthus truncatus seeds to germinate. (3) Indoor cultivation of seedlings: When the seeds of Acanthus truncatus grow 2 to 4 true leaves, select the Acanthus truncatus seedlings that are not bitten by insects and transfer them to the cultivation module in the nursery area. The cultivation medium is sand. Adjust the intensity and time of light, the amount of organic fertilizer applied, the amount of calcium, magnesium, trace elements applied, the salinity of the cultivation medium, the ambient temperature and relative humidity. Harvest after 60 to 90 days of cultivation.

[0007] The carbendazim solution in step (1) is a carbendazim powder containing 50% of the active ingredient dissolved in water at a ratio of 1 g: 500 mL, and the water temperature is 20°C.

[0008] The illumination in step (2) is carried out using an LED light source with an illumination intensity of 50 to 400 μmol·m -2 ·s -1 , the light duration is greater than 6 hours per day; the trace elements include concentrated solution A and concentrated solution B, the application amount is 0.5 mL of concentrated solution A and concentrated solution B per kg of substrate, and it is applied once every 20 days; the environmental temperature for seed germination is 26-32°C, the relative humidity is 70-90%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist.

[0009] The illumination in step (3) is carried out using an LED light source with an illumination intensity of 200 to 400 μmol·m -2 ·s -1 , the lighting duration is 8-12 h / d; the amount of organic fertilizer applied is 5.39 g per kg of cultivation substrate; the amount of calcium element applied is 0.278-1.665 g of CaCl2 per kg of cultivation substrate; the amount of magnesium element applied is 0.308-1.849 g of MgSO4·7H2O per kg of cultivation substrate; the amount of trace elements applied is 0.5-2 mL of concentrate A and concentrate B respectively per kg of cultivation substrate, applied once every 20 days, and the trace elements include concentrate A and concentrate B.

[0010] The salinity of the cultured substrate in step (3) is 0-5‰, the ambient temperature is 28-32°C, the relative humidity is 70-90%, and the evaporated water is replenished every day.

[0011] The concentrated solution A contains 2.860 g / L H3BO3, 1.015 g / L MnSO4, 0.079 g / L CuSO4·5H2O, and 0.220 g / L ZnSO4·7H2O, and the concentrated solution B contains 0.090 g / L H2MoO4, 5.570 g / L FeSO4·7H2O, and 7.450 g / L EDTA-Na2.

[0012] The effective ingredients in the organic fertilizer in step (3) are: 9.91% N, 2.36% P2O5, and 2.50% K2O.

[0013] The spectrum composition of the LED light source is that the proportion of photons with a wavelength of 400-499 nm is 31%, the proportion of photons with a wavelength of 500-599 nm is 11%, the proportion of photons with a wavelength of 600-700 nm is 56%, and the proportion of photons with a wavelength of 701-780 nm is 2%.

[0014] The beneficial effects of the present invention are: The present invention utilizes indoor vertical cultivation facilities to set specific light intensity, photoperiod, temperature, salinity, application amount of organic fertilizer, calcium, magnesium and trace elements, gets rid of the restrictions on soil fertility, climate, sunshine and salinity, and can achieve rapid and high-density cultivation of Acanthus seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a diagram of the module installation for cultivating the mangrove plant Acanthus sphaerocephalus.

[0016] Figure 2 To screen the light duration during the cultivation period of the mangrove plant Acanthus dasyphylla seedlings.

[0017] Figure 3 To screen the light intensity during the cultivation period of the mangrove plant Acanthus sphaerocephalus seedlings.

[0018] Figure 4 To screen the temperature during the cultivation period of the mangrove plant Acanthus sphaerocephalus seedlings.

[0019] Figure 5 To screen the salinity during the cultivation period of the mangrove plant Acanthus sphaerocephalus seedlings.

[0020] Figure 6 To screen the amount of organic fertilizer to be applied during the cultivation period of the mangrove plant Acanthus dasyphylla seedlings.

[0021] Figure 7 To screen the amount of calcium and magnesium to be applied during the cultivation period of the mangrove plant Acanthus dasyphylla seedlings.

[0022] Figure 8 To screen the amount of trace elements to be applied during the cultivation period of the mangrove plant Acanthus dasyphylla seedlings. DETAILED DESCRIPTION

[0023] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0024] A method for indoor seedling cultivation of a mangrove plant Acanthus sphaerocephalus comprises the following steps: (1) Seed pretreatment: Sterilize the Acanthus truncatus seeds with a carbendazim solution for 15 min to obtain pretreated Acanthus truncatus seeds; (2) Seed germination: insert one quarter of the pre-treated Acanthus truncatus seeds into the holes of the seedling tray. The culture medium in the holes is sand. Spray with clean water and place under light. Adjust the environmental temperature and humidity for seed germination, add trace elements, and wait for the Acanthus truncatus seeds to germinate. (3) Indoor cultivation of seedlings: When the seeds of Acanthus truncatus grow 2 to 4 true leaves, select the Acanthus truncatus seedlings that are not bitten by insects and transfer them to the cultivation module in the nursery area. The cultivation medium is sand. Adjust the light intensity and time, the amount of organic fertilizer, the amount of calcium, magnesium, trace elements, the salinity of the cultivation medium and the ambient temperature, and harvest after 60 to 90 days of cultivation.

[0025] The carbendazim solution in step (1) is a carbendazim powder containing 50% of the active ingredient, which is dissolved in water at a ratio of 1 g: 500 mL, and the water temperature is 20°C.

[0026] The illumination in step (2) is carried out using an LED light source with an illumination intensity of 100 μmol·m -2 ·s -1 , the light duration is 12 h per day; the trace elements include concentrated solution A and concentrated solution B, the application amount is 0.5 mL of concentrated solution A and concentrated solution B per kg of substrate, and it is applied once every 20 days; the environmental temperature for seed germination is 28°C, the relative humidity is 70-90%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist.

[0027] The illumination in step (3) is carried out using an LED light source with an illumination intensity of 200 to 400 μmol·m -2 ·s -1 , the lighting duration is 8-12 h / d; the amount of organic fertilizer applied is 5.39 g per kg of cultivation substrate; the amount of calcium element applied is 0.278-1.665 g of CaCl2 per kg of cultivation substrate; the amount of magnesium element applied is 0.308-1.849 g of MgSO4·7H2O per kg of cultivation substrate; the trace elements include concentrated solution A and concentrated solution B, and the application amount is 0.5-2 mL of concentrated solution A and concentrated solution B per kg of cultivation substrate, and it is applied once every 20 days.

[0028] The culture medium in step (3) is sand, the salinity of the culture medium is 0-5‰, the ambient temperature is 28-32°C, the relative humidity is 70-90%, and the evaporated water is replenished every day to maintain the culture conditions within a stable range.

[0029] The concentration of the concentrated solution A is 2.860 g / L H3BO3, 1.015 g / L MnSO4, 0.079 g / L CuSO4·5H2O, 0.220 g / L ZnSO4·7H2O, 0.090 g / L, and the concentration of the concentrated solution B is H2M O O4, 5.570 g / LFeSO4·7H2O, 7.450 g / L EDTA-Na2.

[0030] The effective ingredients in the organic fertilizer are: 9.91% N, 2.36% P2O5, and 2.50% K2O.

[0031] The spectrum composition of the LED lamp is that the proportion of photons with a wavelength of 400-499 nm is 31%, the proportion of photons with a wavelength of 500-599 nm is 11%, the proportion of photons with a wavelength of 600-700 nm is 56%, and the proportion of photons with a wavelength of 701-780 nm is 2%.

[0032] The following examples only list the parts that are different from the above steps, and the same parts are not listed again. At the same time, in order to obtain the best implementation method, a method for indoor seedling cultivation of a mangrove plant Acanthus sphaerocephalus is provided as follows.

[0033] Example 1 Screening of photoperiod for seedling cultivation The light duration was set at 8, 12, 16, and 20 h / d. The light intensity was 150 μmol·m -2 ·s -1 , the ambient temperature was 28°C, the relative humidity was 85%, the salinity was 0‰, and the amount of organic fertilizer applied was 5.39 g / kg substrate.

[0034] Observe the growth of seedlings every day, replenish the evaporated water, and keep the culture conditions within a stable range. Figure 1 ) Harvest was carried out 60 days after planting, and samples were collected at harvest to measure various plant indicators.

[0035] Figure 2 The effect of different light durations on the growth of mangrove plant Acanthus serrata seedlings. Figure 2It can be seen that the plant height increment and leaf length increment of Acanthus truncatus seedlings decreased with the increase of light duration. When the light duration was 8 h / d, the maximum values ​​were 1.45±0.08 cm / plant and 5.07±0.52 cm / leaf, respectively, followed by 12 h / d. There was no significant difference in the leaf width increment under different light durations. The leaf number increment showed a trend of increasing first and then decreasing. When the light duration was 12 h / d, the leaf number increment of Acanthus truncatus seedlings reached a maximum value of 5.33±0.61 leaf / plant, followed by 8 h / d. Therefore, a light duration of 8-12 h / d can significantly increase the growth rate of Acanthus truncatus.

[0036] Example 2 Screening of light intensity for cultivating Acanthus sibiricus seedlings The light intensity was set at 50, 100, 200, and 400 μmol·m -2 ·s -1 The light duration was 12 h / d, the ambient temperature was 28°C, the relative humidity was 85%, the salinity was 0‰, and the amount of organic fertilizer applied was 5.39 g / kg substrate.

[0037] Observe the growth of seedlings every day, replenish evaporated water, and maintain the culture conditions within a stable range. The Acanthus plants were harvested 60 days after being planted in the plant factory module, and samples were collected at the time of harvest to measure various plant indicators.

[0038] Figure 3 The effect of different light intensities on the growth of the mangrove plant Acanthus serrata seedlings. Figure 3 It can be seen that the increment of plant height, leaf length, leaf width and leaf number of Acanthus truncatus seedlings increased first and then decreased with the increase of light intensity, and .... -2 ·s -1 The maximum values ​​were 1.72 ± 0.07 cm / plant, 5.70 ± 0.72 cm / piece, 2.47 ± 0.47 cm / piece, and 7.07 ± 0.23 piece / plant, respectively. Therefore, the light intensity was 200~400 μmol·m -2 ·s -1 It can significantly increase the growth rate of rat lily.

[0039] Example 3 Screening of Cultivation Temperature for Acanthus sibiricus Seedlings The temperature was set at 20°C, 24°C, 28°C, and 32°C. The light duration was 12 h / d, and the light intensity was 150 μmol·m -2 ·s -1 , relative humidity was 85%, salinity was 0‰, and the amount of organic fertilizer applied was 5.39 g / kg.

[0040] The growth of seedlings was observed every day, and the evaporated water was replenished to maintain the culture conditions within a stable range. The Acanthus plants were harvested 90 days after being planted in the plant factory module, and samples were collected during the harvest to measure various plant indicators.

[0041] Figure 4 The effect of different temperatures on the growth of the mangrove plant Acanthus serrata seedlings. Figure 4 It can be seen that the increment of plant height and the increment of leaf number of Acanthus truncatus showed a gradual increasing trend, reaching the maximum values ​​of 7.84 ± 0.48 cm / plant and 5.75 ± 0.00 leaves / plant at 32°C; the increment of leaf length and leaf width showed a trend of first increasing and then decreasing under various temperature conditions, reaching the maximum values ​​of 6.21 ± 0.29 cm / leaf and 2.51 ± 0.04 cm / leaf at 28°C, followed by 32°C. Therefore, the growth rate of Acanthus truncatus was significantly increased when the ambient temperature was 28~32°C.

[0042] Example 4 Screening of salinity for cultivation of Acanthus sphaerocephala seedlings The salinity of the culture medium was set at 0‰, 10‰, 15‰, and 25‰. The light duration was 12 h / d, and the light intensity was 150 μmol·m -2 ·s -1 , the ambient temperature was 28 °C, the relative humidity was 85%, and the amount of organic fertilizer applied was 5.39 g / kg substrate.

[0043] The growth of seedlings was observed every day, and the evaporated water was replenished to maintain the culture conditions within a stable range. The Acanthus plants were harvested 60 days after being planted in the plant factory module, and samples were collected during the harvest to measure various plant indicators.

[0044] Figure 5 The effect of different salinity levels on the growth of mangrove plant Acanthus serrata seedlings. Figure 5 It can be seen that compared with 0‰ salinity, the growth indicators of Acanthus truncatus were inhibited at 10‰, 15‰ and 25‰ salinities and gradually decreased with the increase of salinity, while at 5‰ salinity, the plant height increment, leaf width increment and leaf number increment of Acanthus truncatus reached the maximum values, which were 4.20 ± 0.15 cm / plant, 2.44 ± 0.13 cm / leaf, and 6.00 ± 0.00 leaf / plant, respectively, and the leaf length increment reached the maximum value of 6.07 ± 0.58 cm / leaf at 0‰ salinity. Therefore, the growth rate of Acanthus truncatus was significantly increased at 0-5‰ salinity.

[0045] Example 5 Screening of the amount of organic fertilizer applied to the cultivation of Acanthus sibiricus seedlings The application amount of organic fertilizer was set at 0, 2.70, 5.39, 10.77, and 21.54 g / kg of culture medium. The light duration was 12 h / d, and the light intensity was 150 μmol·m-2 ·s -1 , the ambient temperature is 28℃, the relative humidity is 85%, and the salinity is 0‰. The effective ingredients in organic fertilizer are: 9.91% N, 2.36% P2O5, 2.50% K2O.

[0046] The growth of seedlings was observed every day, and the evaporated water was replenished to maintain the culture conditions within a stable range. The Acanthus plants were harvested 90 days after being planted in the plant factory module, and samples were collected during the harvest to measure various plant indicators.

[0047] Figure 6 The effect of different amounts of organic fertilizer on the growth of mangrove plant Acanthus sibiricus seedlings. Figure 6 It can be seen that the plant height increment of Acanthus truncatus first increased and then decreased with the increase of organic fertilizer application, and the increment of leaf length, leaf width and leaf number had no obvious change pattern under the application of organic fertilizer. The increment of plant height, leaf length and leaf width reached the maximum value when the organic fertilizer application amount was 5.39 g / kg, which were 4.78 ± 0.99 cm / plant, 5.44 ± 0.14 cm / leaf and 2.25 ± 0.09 cm / leaf, respectively. Therefore, the application of organic fertilizer at 5.39 g / kg significantly increased the growth rate of Acanthus truncatus.

[0048] Example 6 Screening of calcium and magnesium application amounts for Acanthus sibiricum seedling cultivation The amount of calcium and magnesium applied was set at 0.5, 1, 2, and 3 times the concentration, with 1 times the concentration being MgSO4·7H2O: 0.612 g / kg culture medium and CaCl2: 0.555 g / kg culture medium. The light duration was 12 h / d, and the light intensity was 150 μmol·m -2 ·s -1 , the ambient temperature was 28°C, the relative humidity was 85%, the salinity was 0‰, and the amount of organic fertilizer applied was 5.39 g / kg substrate.

[0049] The growth of seedlings was observed every day, and the evaporated water was replenished to maintain the culture conditions within a stable range. The Acanthus plants were harvested 90 days after being planted in the plant factory module, and samples were collected during the harvest to measure various plant indicators.

[0050] Figure 7 The effect of different calcium and magnesium application levels on the growth of mangrove plant Acanthus sibiricus seedlings. Figure 7It can be seen that the plant height and leaf length increment of Acanthus truncatus gradually increased with the increase of calcium and magnesium application, and the maximum value was obtained at 3 times the concentration, with the values ​​of 3.73 ± 0.12 cm / plant and 5.51 ± 0.20 cm / leaf respectively; the leaf width increment and leaf number increment had no obvious change pattern under the application of calcium and magnesium elements, and the maximum value was obtained at 0.5 and 2 times the concentration, with the values ​​of 2.39 ± 0.50 cm / leaf and 2.58 ± 0.80 leaf / plant respectively. Therefore, the application of calcium and magnesium elements at 0.5~3 times significantly increased the growth rate of Acanthus truncatus.

[0051] Example 7 Screening of trace element application amount for seedling cultivation The trace element application amount was set as follows: 0.5 (0.5 mL / kg substrate for each concentrate A and B), 1 (1 mL / kg substrate for each concentrate A and B), 2 (2 mL / kg substrate for each concentrate A and B), and 3 times (3 mL / kg substrate for each concentrate A and B), and was applied once every 20 days. The light duration was 12 h / d, and the light intensity was 150 μmol·m -2 ·s -1 , the ambient temperature was 28°C, the relative humidity was 85%, the salinity was 0‰, and the amount of organic fertilizer applied was 5.39 g / kg substrate. Concentrate A was 2.860 g / L H3BO3, 1.015 g / LMnSO4, 0.079 g / L CuSO4·5H2O, and 0.220 g / L ZnSO4·7H2O, and the concentrate B was 0.090 g / L H2MoO4, 5.570 g / L FeSO4·7H2O, and 7.450 g / L EDTA-Na2 The growth of seedlings was observed every day, and the evaporated water was replenished to maintain the culture conditions within a stable range. The Acanthus plants were harvested 90 days after being planted in the plant factory module, and samples were collected during the harvest to measure various plant indicators.

[0052] Figure 8 The effect of different trace element application amounts on the growth of mangrove plant Acanthus serrata seedlings. Figure 8 It can be seen that the increment of plant height and leaf width of Acanthus truncatus showed a trend of first increasing and then decreasing with the increase of trace element application, and the increment of leaf length and leaf number had no obvious change pattern under each trace element application amount. The increment of plant height and leaf width reached the maximum value when the trace element application amount was 0.5 times, and the values ​​were 2.61 ± 0.47 cm / plant and 2.25 ± 0.03 cm / plant, respectively. The increment of leaf length reached the maximum value of 5.14 ± 0.24 cm / plant when the concentration of trace element application was 2 times, followed by 0.5 times. Therefore, the growth rate of Acanthus truncatus was significantly increased when the trace element application amount was 0.5~2 times.

[0053] In summary, the screening of light intensity, photoperiod, temperature, salinity, and application of organic fertilizer, calcium, magnesium, and trace elements (Examples 1-7) in seedling cultivation showed that the light intensity of 200-400 μmol·m -2 ·s -1 When the ambient temperature is 28~32℃, the salinity is 0~5‰, the organic fertilizer application amount is 5.39 g / kg, the calcium and magnesium application amount is 0.5~3 times, and the trace element application amount is 0.5~2 times, the growth indicators of the mangrove plant Acanthus truncatus seedlings are good, which is conducive to the growth of Acanthus truncatus seedlings.

[0054] The above describes the specific content of the present invention through embodiments. For those skilled in the art, it is easy to make several changes and modifications to the content of the present invention based on known principles, so the above embodiments do not represent the entire patent protection scope of the present invention. Therefore, in accordance with the concept limited by the claims, modifications based on the present invention also belong to the protection scope of the claims.

Claims

1. A method for indoor seedling cultivation of a mangrove plant Acanthus sphaerocephalus, characterized in that: The following steps are involved: (1) Seed pretreatment: Sterilize the Acanthus truncatus seeds with a carbendazim solution for 15 min to obtain pretreated Acanthus truncatus seeds; (2) Seed germination: insert one quarter of the pre-treated Acanthus truncatus seeds into the holes of the seedling tray. The culture medium in the holes is sand. Spray with clean water and place under light. Adjust the environmental temperature and relative humidity for seed germination, add trace elements, and wait for the Acanthus truncatus seeds to germinate. (3) Indoor cultivation of seedlings: When the seeds of Acanthus truncatus grow 2 to 4 true leaves, select the Acanthus truncatus seedlings that are not bitten by insects and transfer them to the cultivation module in the nursery area. The cultivation medium is sand. Adjust the intensity and time of light, the amount of organic fertilizer applied, the amount of calcium, magnesium, trace elements applied, the salinity of the cultivation medium, the ambient temperature and relative humidity. Harvest after 60 to 90 days of cultivation.

2. The indoor seedling raising method of a mangrove plant Acanthus sphaerocephalus according to claim 1, characterized in that: The carbendazim solution in step (1) is a carbendazim powder containing 50% of the active ingredient dissolved in water at a ratio of 1 g: 500 mL, and the water temperature is 20°C.

3. The indoor seedling raising method of a mangrove plant Acanthus sphaerocephalus according to claim 1, characterized in that: The illumination in step (2) is carried out using an LED light source with an illumination intensity of 50 to 400 μmol·m -2 ·s -1 , the light duration is greater than 6 hours per day; the trace elements include concentrated solution A and concentrated solution B, the application amount is 0.5 mL of concentrated solution A and concentrated solution B per kg of substrate, and it is applied once every 20 days; the environmental temperature for seed germination is 26-32°C, the relative humidity is 70-90%, and the evaporated water is replenished every day to keep the growth environment of the seeds moist.

4. The indoor seedling raising method of a mangrove plant Acanthus sphaerocephalus according to claim 1, characterized in that: The illumination in step (3) is carried out using an LED light source with an illumination intensity of 200 to 400 μmol·m -2 ·s -1 , the lighting duration is 8-12 h / d; the amount of organic fertilizer applied is 5.39 g per kg of cultivation substrate; the amount of calcium element applied is 0.278-1.665 g of CaCl2 per kg of cultivation substrate; the amount of magnesium element applied is 0.308-1.849 g of MgSO4·7H2O per kg of cultivation substrate; the amount of trace elements applied is 0.5-2 mL of concentrate A and concentrate B respectively per kg of cultivation substrate, applied once every 20 days, and the trace elements include concentrate A and concentrate B.

5. The indoor seedling raising method of a mangrove plant Acanthus sphaerocephalus according to claim 1, characterized in that: The salinity of the cultured substrate in step (3) is 0-5‰, the ambient temperature is 28-32°C, the relative humidity is 70-90%, and the evaporated water is replenished every day.

6. The indoor seedling raising method of the mangrove plant Acanthus sphaerocephalus according to any one of claims 3 or 4, characterized in that: The concentrated solution A contains 2.860 g / L H3BO3, 1.015 g / L MnSO4, 0.079 g / L CuSO4·5H2O, and 0.220 g / L ZnSO4·7H2O, and the concentrated solution B contains 0.090 g / L H2MoO4, 5.570 g / L FeSO4·7H2O, and 7.450 g / L EDTA-Na2.

7. The indoor seedling raising method of a mangrove plant Acanthus sphaerocephalus according to claim 1, characterized in that: The effective ingredients in the organic fertilizer in step (3) are: 9.91% N, 2.36% P2O5, and 2.50% K2O.

8. The indoor seedling raising method of the mangrove plant Acanthus sphaerocephalus according to any one of claims 3 or 4, characterized in that: The spectrum composition of the LED light source is that the proportion of photons with a wavelength of 400-499 nm is 31%, the proportion of photons with a wavelength of 500-599 nm is 11%, the proportion of photons with a wavelength of 600-700 nm is 56%, and the proportion of photons with a wavelength of 701-780 nm is 2%.

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