Polypeptide beverage containing various amino acids and PQQ and preparation method thereof

Abstract

The invention provides a polypeptide beverage containing various amino acids and PQQ and a preparation method of the polypeptide beverage. It is found for the first time that PQQ combined with glutamine, asparagine and lysine can effectively improve the antioxidant activity of the beverage, the beverage has good DPPH free radical scavenging rate and superoxide anion scavenging rate, and a beverage product with nutrition and health functions can be prepared.

Description

Technical Field

[0001] The present invention relates to the field of compound beverages, and more specifically, to a polypeptide beverage containing multiple amino acids and PQQ and a preparation method thereof. Background Art

[0002] Pyrroloquinoline quinone (PQQ) is a redox enzyme cofactor in the body and is known as the fourteenth vitamin. It has a very strong antioxidant capacity, 5,000 times that of vitamin B, and can effectively remove free radicals in the body, enhance the activity of intracellular antioxidant enzymes such as superoxide dismutase and glutathione peroxidase, and reduce oxidative stress damage to mitochondrial structure. In addition, it also improves energy metabolism, promotes growth and reproduction, and protects nerves and cardiovascular.

[0003] Amino acids are the basic units that make up proteins. They are composed of an amino group and a carboxyl group, and usually have a side chain. Amino acids are important organic molecules in living organisms and can participate in building cell structures, regulating the metabolic processes of living organisms, and transmitting signals. In living organisms, amino acids can be synthesized through protein synthesis pathways or ingested through diet. Common amino acids include lysine, phenylalanine, serine, etc.

[0004] Antioxidant drinks can effectively clear free radicals produced in the body, inhibit lipid peroxidation, and play an important role in improving the body's resistance to aging and disease. Summary of the invention

[0005] In view of the technical problems existing in the prior art, the present invention provides a polypeptide beverage containing multiple amino acids and PQQ and a preparation method thereof. The present invention first discovered that PQQ combined with glutamine, asparagine, and lysine can effectively improve the antioxidant activity of the beverage, has good DPPH free radical scavenging rate and superoxide anion scavenging rate, and can prepare a beverage product with nutritional and health functions.

[0006] Specifically, the present invention first provides a polypeptide drink containing multiple amino acids and PQQ, which includes, in parts by mass: 0.001-0.002 parts by mass of PQQ, 2-7 parts by mass of glutamine, 2-7 parts by mass of asparagine, 2-7 parts by mass of lysine, 0.005-0.01 parts by mass of xylitol, 0.005-0.01 parts by mass of citric acid, 0.005-0.01 parts by mass of sodium benzoate, 0.05-0.1 parts by mass of vitamin B, 0.002-0.008 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

[0007] Preferably, the polypeptide beverage containing multiple amino acids and PQQ includes, in parts by mass: 0.001 parts by mass of PQQ, 2 parts by mass of glutamine, 2 parts by mass of asparagine, 2 parts by mass of lysine, 0.005 parts by mass of xylitol, 0.005 parts by mass of citric acid, 0.005 parts by mass of sodium benzoate, 0.05 parts by mass of vitamin B, 0.002 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

[0008] Preferably, the polypeptide beverage containing multiple amino acids and PQQ includes, in parts by mass: 0.0015 parts by mass of PQQ, 5 parts by mass of glutamine, 5 parts by mass of asparagine, 5 parts by mass of lysine, 0.008 parts by mass of xylitol, 0.008 parts by mass of citric acid, 0.008 parts by mass of sodium benzoate, 0.08 parts by mass of vitamin B, 0.006 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

[0009] Preferably, the polypeptide beverage containing multiple amino acids and PQQ includes, in parts by mass: 0.002 parts by mass of PQQ, 7 parts by mass of glutamine, 7 parts by mass of asparagine, 7 parts by mass of lysine, 0.01 parts by mass of xylitol, 0.01 parts by mass of citric acid, 0.01 parts by mass of sodium benzoate, 0.1 parts by mass of vitamin B, 0.008 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

[0010] On the other hand, the present invention also provides a method for preparing a polypeptide beverage containing multiple amino acids and PQQ, characterized in that the method comprises the following steps:

[0011] 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 60-75°C, and stir thoroughly to dissolve;

[0012] 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use;

[0013] 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

[0014] Preferably, the method for preparing the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps:

[0015] 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 60°C, and stir thoroughly to dissolve;

[0016] 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use;

[0017] 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and then homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

[0018] Preferably, the method for preparing the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps:

[0019] 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 65°C, and stir thoroughly to dissolve;

[0020] 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use;

[0021] 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

[0022] Preferably, the method for preparing the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps:

[0023] 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 70°C, and stir thoroughly to dissolve;

[0024] 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use;

[0025] 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and then homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

[0026] Furthermore, the present invention also provides the use of a combination of PQQ and glutamine, asparagine and lysine in preparing a polypeptide drink.

[0027] Preferably, the combination of PQQ and glutamine, asparagine and lysine can effectively improve the antioxidant activity of the polypeptide beverage.

[0028] The advantages of the invention are as follows: The present invention is the first to discover that PQQ combined with glutamine, asparagine, and lysine can effectively improve the antioxidant activity of beverages, has good DPPH free radical scavenging rate and superoxide anion scavenging rate, and can prepare a beverage product with nutritional and health functions. DETAILED DESCRIPTION

[0029] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand the present invention more clearly.

[0030] The following embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. Based on the specific embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work belong to the protection scope of the present invention.

[0031] In the examples of the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art; in the examples of the present invention, unless otherwise specified, the technical means used are conventional means well known to those skilled in the art.

[0032] Example 1

[0033] A polypeptide drink containing multiple amino acids and PQQ, comprising, in parts by mass: 0.001 parts by mass of PQQ, 2 parts by mass of glutamine, 2 parts by mass of asparagine, 2 parts by mass of lysine, 0.005 parts by mass of xylitol, 0.005 parts by mass of citric acid, 0.005 parts by mass of sodium benzoate, 0.05 parts by mass of vitamin B, 0.002 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

[0034] The preparation method of the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps:

[0035] 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 60°C, and stir thoroughly to dissolve;

[0036] 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use;

[0037] 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

[0038] Example 2

[0039] A polypeptide drink containing multiple amino acids and PQQ, comprising, in parts by mass: 0.0015 parts by mass of PQQ, 5 parts by mass of glutamine, 5 parts by mass of asparagine, 5 parts by mass of lysine, 0.008 parts by mass of xylitol, 0.008 parts by mass of citric acid, 0.008 parts by mass of sodium benzoate, 0.08 parts by mass of vitamin B, 0.006 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

[0040] The preparation method of the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps:

[0041] 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 65°C, and stir thoroughly to dissolve;

[0042] 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use;

[0043] 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and then homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

[0044] Example 3

[0045] A polypeptide drink containing multiple amino acids and PQQ, comprising, in parts by mass: 0.002 parts by mass of PQQ, 7 parts by mass of glutamine, 7 parts by mass of asparagine, 7 parts by mass of lysine, 0.01 parts by mass of xylitol, 0.01 parts by mass of citric acid, 0.01 parts by mass of sodium benzoate, 0.1 parts by mass of vitamin B, 0.008 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

[0046] The preparation method of the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps:

[0047] 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 70°C, and stir thoroughly to dissolve;

[0048] 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use;

[0049] 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and then homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

[0050] Comparative Example 1

[0051] A beverage, the formula and preparation method of which are the same as those of Example 1, except that the beverage does not contain PQQ.

[0052] Comparative Example 2

[0053] A beverage, the formula and preparation method of which are the same as those of Example 1, except that the beverage does not contain glutamine, asparagine or lysine.

[0054] Comparative Example 3

[0055] A beverage, the formula and preparation method of which are the same as those of Example 1, except that the beverage does not contain PQQ, glutamine, asparagine, or lysine.

[0056] Verification Example

[0057] 1. DPPH free radical scavenging assay

[0058] Treatment group: Take 1 mL of each of the beverages of Examples 1-3 and Comparative Examples 1-3, add 1 mL of 0.5 mol / L DPPH solution, mix well, react at room temperature in the dark for 45 minutes, centrifuge at 5000 rpm for 10 minutes, and measure the absorbance A1 at a wavelength of 517 nm. Blank group: Add 1 mL of 0.5 mol / L DPPH solution to 1 mL of deionized water. Mix well, react at room temperature in the dark for 30 minutes, centrifuge at 5000 rpm for 5 minutes, and measure the absorbance A0 at a wavelength of 517 nm. The calculation formula is shown in Formula I: DPPH free radical scavenging rate (%) = (1-(A1÷A0))×100% (Formula I).

[0059] 2. Superoxide Anion Radical Scavenging Assay

[0060] Treatment group: 5 mL of 0.05 mol / L Tris-HCl buffer (pH 8.5) was taken, placed in a 25°C water bath for preheating for 30 min, 4 mL of the beverages of Examples 1-3 and Comparative Examples 1-3 were added, 1 mL of 3 mmol / L pyrogallol solution was added, mixed, placed in a 25°C water bath for 5 min, 1 mL of 10 mol / L HCl was added to terminate the reaction, and the absorbance B1 was measured at 320 nm; Blank group: The composite beverage was replaced with the same volume of distilled water, and after the same treatment, the absorbance B0 was measured at 320 nm. The calculation formula is shown in Formula II: Superoxide anion radical scavenging (%) = (1-(B1÷B0)) × 100% (Formula II).

[0061] Table 1. Activity analysis of beverages

[0062] DPPH free radical scavenging rate (%) Superoxide anion clearance rate (%) Example 1 80.78±3.55 25.68±1.25 Example 2 85.45±2.12 35.57±1.24 Example 3 95.23±1.25 40.78±2.12 Comparative Example 1 35.27±1.68 18.56±2.45 Comparative Example 2 20.54±3.25 5.54±0.75 Comparative Example 3 2.24±0.23 0.89±0.05

[0063] As shown in Table 1, the peptide beverages containing PQQ and various amino acids described in Examples 1-3 of the present invention have excellent antioxidant activity, high DPPH free radical scavenging rate and superoxide anion scavenging rate, and are beverage products with nutritional and health functions. In particular, there is a synergistic effect between PQQ and various amino acids, which can effectively improve the antioxidant activity of the beverage.

[0064] It is necessary to point out that the above embodiments are limited to further elaboration and explanation of the technical solution of the present invention, and are not further limitations of the technical solution of the present invention. The method of the present invention is only a preferred implementation scheme, and is not used to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A polypeptide drink containing multiple amino acids and PQQ, characterized in that: In parts by mass, the invention comprises: 0.001-0.002 parts by mass of PQQ, 2-7 parts by mass of glutamine, 2-7 parts by mass of asparagine, 2-7 parts by mass of lysine, 0.005-0.01 parts by mass of xylitol, 0.005-0.01 parts by mass of citric acid, 0.005-0.01 parts by mass of sodium benzoate, 0.05-0.1 parts by mass of vitamin B, 0.002-0.008 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

2. The beverage according to claim 1, characterized in that The polypeptide drink containing multiple amino acids and PQQ includes, in parts by mass: 0.001 parts by mass of PQQ, 2 parts by mass of glutamine, 2 parts by mass of asparagine, 2 parts by mass of lysine, 0.005 parts by mass of xylitol, 0.005 parts by mass of citric acid, 0.005 parts by mass of sodium benzoate, 0.05 parts by mass of vitamin B, 0.002 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

3. The beverage according to claim 1, characterized in that The polypeptide drink containing multiple amino acids and PQQ includes, in parts by mass: 0.0015 parts by mass of PQQ, 5 parts by mass of glutamine, 5 parts by mass of asparagine, 5 parts by mass of lysine, 0.008 parts by mass of xylitol, 0.008 parts by mass of citric acid, 0.008 parts by mass of sodium benzoate, 0.08 parts by mass of vitamin B, 0.006 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

4. The beverage according to claim 1, characterized in that The polypeptide drink containing multiple amino acids and PQQ includes, in parts by mass: 0.002 parts by mass of PQQ, 7 parts by mass of glutamine, 7 parts by mass of asparagine, 7 parts by mass of lysine, 0.01 parts by mass of xylitol, 0.01 parts by mass of citric acid, 0.01 parts by mass of sodium benzoate, 0.1 parts by mass of vitamin B, 0.008 parts by mass of potassium sorbate and 100 parts by mass of sterile water.

5. The method for preparing a polypeptide beverage containing multiple amino acids and PQQ according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 60-75°C, and stir thoroughly to dissolve; 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use; 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

6. The method according to claim 5, characterized in that The preparation method of the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps: 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 60°C, and stir thoroughly to dissolve; 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use; 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

7. The method according to claim 5, characterized in that The preparation method of the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps: 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 65°C, and stir thoroughly to dissolve; 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use; 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

8. The method according to claim 5, characterized in that The preparation method of the polypeptide beverage containing multiple amino acids and PQQ comprises the following steps: 1) Weigh PQQ, glutamine, asparagine, lysine, sodium benzoate, and potassium sorbate according to the number of portions, dissolve them in sterile water, mix well, heat to 70°C, and stir thoroughly to dissolve; 2) Weigh xylitol, citric acid and vitamin B in portions, and stir and mix them evenly for later use; 3) The mixed solution obtained in step 1) is mixed with the mixed solution obtained in step 2), stirred evenly and homogenized to obtain a polypeptide drink containing multiple amino acids and PQQ.

9. Use of a combination of PQQ and glutamine, asparagine and lysine in the preparation of a polypeptide drink.

10. The use according to claim 9, characterized in that The combination of PQQ and glutamine, asparagine and lysine can effectively improve the antioxidant activity of the polypeptide drink.