Acidified soil conditioner and preparation method thereof

Abstract

The invention relates to an acidified soil conditioner and a preparation method thereof, and belongs to the technical field of soil conditioners. The acidic soil conditioner disclosed by the invention is prepared from the following raw materials in parts by weight: 10 to 15 parts of bacillus subtilis, 10 to 15 parts of EM bacteria, 20 to 30 parts of ethylhexylglycerin, 3 to 8 parts of chitin, 10 to 15 parts of sodium tripolyphosphate, 25 to 35 parts of calcium magnesium phosphate fertilizer, 12 to 15 parts of allantoin, 20 to 30 parts of modified oyster shells and 35 to 45 parts of modified brewer's grains. The soil conditioner disclosed by the invention can be used for improving the acidity of soil, increasing the pH of the soil, increasing the content of nutritional ingredients such as organic matters and reducing the content of heavy metals, and is beneficial to crop growth and improving the quality and yield of crops.

Description

Technical Field

[0001] The invention belongs to the technical field of soil conditioners and relates to an acidified soil conditioner and a preparation method thereof. Background Art

[0002] Soil acidification refers to the process in which the soil absorptive complex accepts a certain amount of exchangeable hydrogen ions or aluminum ions, causing the leaching of alkaline (base) ions in the soil.

[0003] 1. Large and concentrated precipitation, strong leaching, and a large loss of alkaline salts such as calcium, magnesium, and potassium are the fundamental causes of soil acidification. 2. The lack of traditional agricultural measures such as lime application, burning manure, and organic fertilizer application has caused an imbalance of nutrients in cultivated soil, which is the main cause of soil acidification. 3. Long-term and large-scale application of chemical fertilizers is an important cause of soil acidification. Even long-term application of urea can cause soil acidification.

[0004] The pH value of soil is crucial for plant growth. Most crops thrive in neutral soils with a pH value of 7 or slightly acidic soils. Once the pH value of the soil drops, the diseases and pests it brings will hinder plant growth, such as the growth of a plant's root system, affecting the plant's absorption of nutrients, resulting in reduced crop yields and quality; at the same time, soil acidification will increase the toxicity of soil metals, and a strong acid environment will accelerate the leaching of toxic metals into surrounding water bodies.

[0005] The application of quicklime is currently the main measure to improve acidic soil in agricultural production, but the effect of lime improvement is poorly sustained, and improper application can easily lead to soil compaction and nutrient imbalance. The main lime improvers on the market can be divided into four categories: carbonates, oxides, hydroxides, and by-products. According to their ability to neutralize acids, from strong to weak, oxides ≥ hydroxides > carbonates ≥ lime by-products. However, although simple oxides and hydroxides can quickly increase soil pH, they have obvious side effects. They absorb moisture and generate high temperatures in the process of neutralizing acids, which affects crops. In addition, excessive application will reduce soil organic matter and cause soil compaction. Summary of the invention

[0006] The main purpose of the present invention is to provide an acidic soil conditioner, which can significantly increase the pH value of acidic soil, reduce the degree of soil acidification, and improve the physical and chemical properties of the soil.

[0007] The present invention adopts the following technical solutions to achieve the above purpose:

[0008] An acidic soil conditioner is mainly prepared from the following raw materials in parts by weight:

[0009] 10-15 parts of Bacillus subtilis, 10-15 parts of EM bacteria, 20-30 parts of ethylhexylglycerol, 3-8 parts of chitin, 10-15 parts of sodium tripolyphosphate, 25-35 parts of calcium magnesium phosphate fertilizer, 12-15 parts of allantoin, 20-30 parts of modified oyster shells, and 35-45 parts of modified brewer's grains.

[0010] Furthermore, in the acidic soil conditioner, the effective viable bacteria count of Bacillus subtilis is ≥ 20 billion / g, and the effective viable bacteria count of EM bacteria is ≥ 20 billion / g.

[0011] Furthermore, the preparation method of the modified oyster shell powder is:

[0012] The oyster shell powder is soaked in a calcium hydroxide solution with a mass fraction of 10-15% for 10-12 hours, filtered, calcined in an oxygen-free environment at 700-800°C for 3-4 hours, then cooled to 300-400°C, sepiolite is added, kept warm for 1-1.5 hours, then cooled to 200-250°C, polyethylene glycol in a molten state is added, sodium carboxymethyl cellulose is added after mixing, mixed, cooled to room temperature, and crushed to obtain modified oyster shell powder.

[0013] Furthermore, in the above-mentioned method for preparing modified oyster shell powder, the mass ratio of oyster shell powder, sepiolite, polyethylene glycol and sodium carboxymethyl cellulose is 1:(0.1-0.15):(0.8-1):(0.3-0.5).

[0014] Further, the preparation method of modified brewer's grains is:

[0015] The brewer's grains are inoculated with an Aspergillus niger suspension at a solid-liquid ratio of 1:0.3 (g / mL), the pH is adjusted to 7.0-7.5, fermented and cultured at 25-30°C for 1-1.5 days, boiled with water, cooled to room temperature, adjusted to pH 5.0-6.0, cellulase, β-glucosidase, and Bacillus subtilis protease are added, enzymolysis is carried out at 35-40°C for 6-8 hours, boiled again, cooled to room temperature, and dried to obtain product I for standby use; 0.1 mol / L NaOH solution is added to product I at a solid-liquid ratio of 1:30 (g / mL), placed in a 40°C water bath for insulation for 3-4 hours, filtered, and a mixed solution containing triethanolamine and polyvinyl pyrrolidone is added at a solid-liquid ratio of 1:4 (g / mL), mixed well, and dried to obtain modified brewer's grains.

[0016] Furthermore, in the modified brewer's grains preparation method, the amount of Aspergillus niger spores in the Aspergillus niger suspension is 5×10 10 Pieces / mL.

[0017] Furthermore, in the modified brewer's grain preparation method, the added amount of cellulase is 5-8% of the mass of the brewer's grain, the added amount of β-glucosidase is 4-7% of the mass of the brewer's grain, and the added amount of Bacillus subtilisin is 10-12% of the mass of the brewer's grain.

[0018] Furthermore, in the modified brewer's grains preparation method, the mass fraction of triethanolamine in the mixed solution is 10-15%, and the mass fraction of polyvinyl pyrrolidone is 25-30%.

[0019] The present invention provides a method for preparing the acidic soil conditioner, comprising the following steps:

[0020] Step A, mixing Bacillus subtilis, EM bacteria, chitin and ethylhexylglycerol, drying and crushing to obtain mixture I;

[0021] Step B, mixing mixture I, sodium tripolyphosphate, calcium magnesium phosphate fertilizer, allantoin, modified oyster shell powder and modified brewer's grains to obtain an acidic soil conditioner.

[0022] The present invention has the following beneficial effects:

[0023] 1. The main component of the oyster shell used in the present invention is calcium carbonate, which will decompose to produce calcium oxide after high-temperature calcination. Polyethylene glycol and sodium carboxymethyl cellulose are used to seal it, so as to avoid the reaction of calcium oxide with a large amount of water to generate strong alkaline calcium hydroxide, release high heat, and cause harm to crops; in actual use, the adsorption properties of polyethylene glycol, sodium carboxymethyl cellulose, and sepiolite are utilized to preserve moisture, so that it slowly contacts with calcium oxide and acts slowly. At the same time, this is a long-term and continuous process, and a small amount of continuous reaction can generate calcium hydroxide, which can improve the soil pH for a long time.

[0024] 2. The conditioner of the present invention also creatively adds alkaline modified brewer's grains. During the modification process, enzymatic hydrolysis is used to decompose some macromolecular substances in the brewer's grains into small molecular substances, which can effectively increase the nutritional components in the soil. When it is used in the soil, the alkaline components can neutralize the acidic substances in the soil. The modified brewer's grains have the dual functions of improving the pH of the soil and providing nutrients.

[0025] 3. In the present invention, microorganisms and alkaline substances work together. Both microorganisms and modified oyster shell powder can continuously and long-term improve the soil pH, and are the main active components of the second gradient; the other components are the main components of the first gradient, which can reduce soil acidity and provide nutrients for crops when applied. DETAILED DESCRIPTION

[0026] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications of various equivalent forms of the present invention by those skilled in the art all fall within the scope of protection of the claims of this application. In the following implementation manner, the preparation method of the Aspergillus niger suspension is to activate the Aspergillus niger strain with a deposit number of CGMCC 3.15297 deposited from the China General Microbiological Culture Collection Center according to the instructions, expand the culture to prepare a seed solution, and then dilute it to prepare a spore amount of 5×10 10 / mL of Aspergillus niger suspension; the cellulase CAS number is 9012-54-8, the enzyme activity is 50u / mg, the β-glucosidase CAS number is 9001-22-3, the enzyme activity is 100u / g, the subtilis protease CAS number is 9014-01-1, the enzyme activity is 150U / mg, and they can be obtained through online or offline channels; Bacillus subtilis and EM bacteria are both existing commercially available products, and in the present invention, they were purchased from Shandong Runwo Biotechnology Co., Ltd.

[0027] Example 1

[0028] Preparation of modified oyster shell powder:

[0029] Take oyster shells, crush and sieve to obtain oyster shell powder; soak 1 kg of oyster shell powder in a 10% by mass calcium hydroxide solution for 12 hours, filter, calcine in an oxygen-free environment at 800°C for 3 hours, then cool to 400°C, add 0.1 kg of sepiolite, keep warm for 1 hour, then cool to 250°C, add 1 kg of molten polyethylene glycol, add 0.3 kg of sodium carboxymethyl cellulose after mixing, mix well, cool to room temperature, and crush to obtain modified oyster shell powder.

[0030] Preparation of modified brewer's grains:

[0031] 1 kg of brewer's grains was inoculated with 5 × 10 spores at a solid-liquid ratio of 1:0.3 (g / mL). 10 The method comprises the following steps: adding a suspension of Aspergillus niger of 1 g / mL, adjusting the pH to 7.5, fermenting and culturing at 30°C for 1 day, adding water to boil, cooling to room temperature, adjusting the pH to 6.0, adding 80 g of cellulase, 40 g of β-glucosidase, and 100 g of Bacillus subtilis protease, performing enzymolysis at 40°C for 6 hours, boiling again, cooling to room temperature, and drying to obtain product I for use; adding 0.1 mol / L NaOH solution to product I at a solid-liquid ratio of 1:30 (g / mL), placing the product I in a water bath at 40°C for insulation for 4 hours, filtering, adding a mixed solution containing 15% triethanolamine and 25% polyvinyl pyrrolidone by mass fraction at a solid-liquid ratio of 1:4 (g / mL), mixing well, and drying to obtain modified brewer's grains.

[0032] To prepare an acidic soil conditioner:

[0033] Step A, 15 kg of Bacillus subtilis with an effective viable count of ≥ 20 billion / g, 10 kg of EM bacteria with an effective viable count of ≥ 20 billion / g, 8 kg of chitin, and 20 kg of ethylhexylglycerol are mixed, dried, and crushed to obtain a mixture I;

[0034] Step B, mixing mixture I, 10 kg of sodium tripolyphosphate, 35 kg of calcium magnesium phosphate fertilizer, 12 kg of allantoin, 30 kg of modified oyster shell powder, and 35 kg of modified brewer's grains to obtain an acidic soil conditioner.

[0035] Example 2

[0036] Preparation of modified oyster shell powder:

[0037] Take oyster shells, crush and sieve to obtain oyster shell powder; soak 1 kg of oyster shell powder in a 15% by mass calcium hydroxide solution for 10 hours, filter, calcine in an oxygen-free environment at 700°C for 4 hours, then cool to 300°C, add 0.15 kg of sepiolite, keep warm for 1.5 hours, cool to 200°C, add 0.8 kg of molten polyethylene glycol, add 0.5 kg of sodium carboxymethyl cellulose after mixing, mix well, cool to room temperature, and crush to obtain modified oyster shell powder.

[0038] Preparation of modified brewer's grains:

[0039] 1 kg of brewer's grains was inoculated with 5 × 10 spores at a solid-liquid ratio of 1:0.3 (g / mL). 10 The method comprises the following steps: adding a suspension of Aspergillus niger of 1 g / mL, adjusting the pH to 7.0, fermenting and culturing at 25°C for 1.5 days, adding water to boil, cooling to room temperature, adjusting the pH to 5.0, adding 50 g of cellulase, 70 g of β-glucosidase, and 120 g of Bacillus subtilis protease, performing enzymolysis at 35°C for 8 hours, boiling again, cooling to room temperature, and drying to obtain product I for use; adding 0.1 mol / L NaOH solution to product I at a solid-liquid ratio of 1:30 (g / mL), placing the product I in a water bath at 40°C for insulation for 3 hours, filtering, adding a mixed solution containing 10% triethanolamine and 30% polyvinyl pyrrolidone by mass fraction at a solid-liquid ratio of 1:4 (g / mL), mixing well, and drying to obtain modified brewer's grains.

[0040] To prepare an acidic soil conditioner:

[0041] Step A, 10 kg of Bacillus subtilis with an effective viable count of ≥ 20 billion / g, 15 kg of EM bacteria with an effective viable count of ≥ 20 billion / g, 3 kg of chitin, and 30 kg of ethylhexylglycerol are mixed, dried, and crushed to obtain a mixture I;

[0042] Step B, mixing mixture I, 15 kg of sodium tripolyphosphate, 25 kg of calcium magnesium phosphate fertilizer, 15 kg of allantoin, 20 kg of modified oyster shell powder, and 45 kg of modified brewer's grains to obtain an acidic soil conditioner.

[0043] Example 3

[0044] Preparation of modified oyster shell powder:

[0045] Take oyster shells, crush and sieve to obtain oyster shell powder; soak 1 kg of oyster shell powder in a 13% by mass calcium hydroxide solution for 11 hours, filter, calcine in an oxygen-free environment at 800°C for 4 hours, then cool to 300°C, add 0.13 kg of sepiolite, keep warm for 1.5 hours, then cool to 250°C, add 0.9 kg of molten polyethylene glycol, add 0.4 kg of sodium carboxymethyl cellulose after mixing, mix well, cool to room temperature, and crush to obtain modified oyster shell powder.

[0046] Preparation of modified brewer's grains:

[0047] 1 kg of brewer's grains was inoculated with 5 × 10 spores at a solid-liquid ratio of 1:0.3 (g / mL). 10 The method comprises the following steps: adding a suspension of Aspergillus niger of 1 g / mL, adjusting the pH to 7.0, fermenting and culturing at 30°C for 1 day, adding water to boil, cooling to room temperature, adjusting the pH to 6.0, adding 70 g of cellulase, 50 g of β-glucosidase, and 110 g of Bacillus subtilis protease, performing enzymolysis at 40°C for 6 h, boiling again, cooling to room temperature, and drying to obtain product I for use; adding 0.1 mol / L NaOH solution to product I at a solid-liquid ratio of 1:30 (g / mL), placing the product I in a water bath at 40°C for insulation for 4 h, filtering, adding a mixed solution containing 13% triethanolamine and 28% polyvinyl pyrrolidone by mass fraction at a solid-liquid ratio of 1:4 (g / mL), mixing well, and drying to obtain modified brewer's grains.

[0048] To prepare an acidic soil conditioner:

[0049] Step A, 13 kg of Bacillus subtilis with an effective viable count of ≥ 20 billion / g, 12 kg of EM bacteria with an effective viable count of ≥ 20 billion / g, 6 kg of chitin, and 25 kg of ethylhexylglycerol are mixed, dried, and crushed to obtain a mixture I;

[0050] Step B, mixing mixture I, 13 kg of sodium tripolyphosphate, 30 kg of calcium magnesium phosphate fertilizer, 14 kg of allantoin, 25 kg of modified oyster shell powder, and 40 kg of modified brewer's grains to obtain an acidic soil conditioner.

[0051] Comparative Example 1

[0052] Preparation of modified brewer's grains:

[0053] 1 kg of brewer's grains was inoculated with 5 × 10 spores at a solid-liquid ratio of 1:0.3 (g / mL). 10 The method comprises the following steps: adding a suspension of Aspergillus niger of 1 g / mL, adjusting the pH to 7.0, fermenting and culturing at 30°C for 1 day, adding water to boil, cooling to room temperature, adjusting the pH to 6.0, adding 70 g of cellulase, 50 g of β-glucosidase, and 110 g of Bacillus subtilis protease, performing enzymolysis at 40°C for 6 h, boiling again, cooling to room temperature, and drying to obtain product I for use; adding 0.1 mol / L NaOH solution to product I at a solid-liquid ratio of 1:30 (g / mL), placing the product I in a water bath at 40°C for insulation for 4 h, filtering, adding a mixed solution containing 13% triethanolamine and 28% polyvinyl pyrrolidone by mass fraction at a solid-liquid ratio of 1:4 (g / mL), mixing well, and drying to obtain modified brewer's grains.

[0054] To prepare an acidic soil conditioner:

[0055] Step A, 13 kg of Bacillus subtilis with an effective viable count of ≥ 20 billion / g, 12 kg of EM bacteria with an effective viable count of ≥ 20 billion / g, 6 kg of chitin, and 25 kg of ethylhexylglycerol are mixed, dried, and crushed to obtain a mixture I;

[0056] Step B, mixing mixture I, 13 kg of sodium tripolyphosphate, 30 kg of calcium magnesium phosphate fertilizer, 14 kg of allantoin, 25 kg of oyster shell powder, and 40 kg of modified brewer's grains to obtain an acidic soil conditioner.

[0057] Comparative Example 2

[0058] Preparation of modified oyster shell powder:

[0059] Take oyster shells, crush and sieve to obtain oyster shell powder; soak 1 kg of oyster shell powder in a 13% by mass calcium hydroxide solution for 11 hours, filter, calcine in an oxygen-free environment at 800°C for 4 hours, then cool to 300°C, add 0.13 kg of sepiolite, keep warm for 1.5 hours, then cool to 250°C, add 0.9 kg of molten polyethylene glycol, add 0.4 kg of sodium carboxymethyl cellulose after mixing, mix well, cool to room temperature, and crush to obtain modified oyster shell powder.

[0060] To prepare an acidic soil conditioner:

[0061] Step A, 13 kg of Bacillus subtilis with an effective viable count of ≥ 20 billion / g, 12 kg of EM bacteria with an effective viable count of ≥ 20 billion / g, 6 kg of chitin, and 25 kg of ethylhexylglycerol are mixed, dried, and crushed to obtain a mixture I;

[0062] Step B, mixing mixture I, 13 kg of sodium tripolyphosphate, 30 kg of calcium magnesium phosphate fertilizer, 14 kg of allantoin, 25 kg of modified oyster shell powder, and 40 kg of brewer's grains to obtain an acidic soil conditioner.

[0063] Comparative Example 3

[0064] Preparation of modified oyster shell powder:

[0065] Take oyster shells, crush and sieve to obtain oyster shell powder; soak 1 kg of oyster shell powder in a 13% calcium hydroxide solution for 11 hours, filter, calcine in an oxygen-free environment at 800°C for 4 hours, cool to room temperature, and crush to obtain modified oyster shell powder.

[0066] Preparation of modified brewer's grains:

[0067] 1 kg of brewer's grains was inoculated with 5 × 10 spores at a solid-liquid ratio of 1:0.3 (g / mL). 10 The method comprises the following steps: adding a suspension of Aspergillus niger of 1 g / mL, adjusting the pH to 7.0, fermenting and culturing at 30°C for 1 day, adding water to boil, cooling to room temperature, adjusting the pH to 6.0, adding 70 g of cellulase, 50 g of β-glucosidase, and 110 g of Bacillus subtilis protease, performing enzymolysis at 40°C for 6 h, boiling again, cooling to room temperature, and drying to obtain product I for use; adding 0.1 mol / L NaOH solution to product I at a solid-liquid ratio of 1:30 (g / mL), placing the product I in a water bath at 40°C for insulation for 4 h, filtering, adding a mixed solution containing 13% triethanolamine and 28% polyvinyl pyrrolidone by mass fraction at a solid-liquid ratio of 1:4 (g / mL), mixing well, and drying to obtain modified brewer's grains.

[0068] To prepare an acidic soil conditioner:

[0069] Step A, 13 kg of Bacillus subtilis with an effective viable count of ≥ 20 billion / g, 12 kg of EM bacteria with an effective viable count of ≥ 20 billion / g, 6 kg of chitin, and 25 kg of ethylhexylglycerol are mixed, dried, and crushed to obtain a mixture I;

[0070] Step B, mixing mixture I, 13 kg of sodium tripolyphosphate, 30 kg of calcium magnesium phosphate fertilizer, 25 kg of modified oyster shell powder, and 40 kg of modified brewer's grains to obtain an acidic soil conditioner.

[0071] Comparative Example 4

[0072] Preparation of modified oyster shell powder:

[0073] Take oyster shells, crush and sieve to obtain oyster shell powder; soak 1 kg of oyster shell powder in a 13% by mass calcium hydroxide solution for 11 hours, filter, calcine in an oxygen-free environment at 800°C for 4 hours, then cool to 300°C, add 0.13 kg of sepiolite, keep warm for 1.5 hours, then cool to 250°C, add 0.9 kg of molten polyethylene glycol, add 0.4 kg of sodium carboxymethyl cellulose after mixing, mix well, cool to room temperature, and crush to obtain modified oyster shell powder.

[0074] Preparation of modified brewer's grains:

[0075] The brewer's grains were added with 0.1 mol / L NaOH solution at a solid-liquid ratio of 1:30 (g / mL), placed in a 40°C water bath for 4 hours, filtered, and a mixed solution containing 13% triethanolamine and 28% polyvinyl pyrrolidone by mass was added at a solid-liquid ratio of 1:4 (g / mL). After mixing, the mixture was dried to obtain the modified brewer's grains.

[0076] To prepare an acidic soil conditioner:

[0077] An acidic soil conditioner is obtained by mixing 13 kg of Bacillus subtilis with an effective live bacteria count ≥ 20 billion / g, 12 kg of EM bacteria with an effective live bacteria count ≥ 20 billion / g, 6 kg of chitin, 30 kg of calcium magnesium phosphate fertilizer, 14 kg of allantoin, 25 kg of modified oyster shell powder, and 40 kg of modified brewer's grains.

[0078] Performance Testing

[0079] 1. Effects of soil conditioners on soil physical and chemical properties and the growth of pakchoi

[0080] 1. Test and Methods

[0081] This experiment was conducted in an agricultural greenhouse in Linyi City. The soil properties were as follows: pH 5.03, organic matter 19.6 g / kg, alkaline nitrogen 127 mg / kg, available phosphorus 86.2 mg / kg, available potassium 138 mg / kg, exchangeable calcium 519 mg / kg, exchangeable magnesium 72.4 mg / kg, chromium 0.402 mg / kg, lead 23.7 mg / kg, mercury 0.0125 mg / kg, and arsenic 0.241 mg / kg.

[0082] The soil was treated as follows and placed in seedling pots, 2 kg / pot, and cultured at room temperature for 10 days.

[0083] Blank group: soil + ordinary organic fertilizer (10g / kg);

[0084] Test group 1: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of Example 1 (10 g / kg);

[0085] Test group 2: soil + common organic fertilizer (10 g / kg) + soil conditioner of Example 2 (10 g / kg);

[0086] Test group 3: soil + common organic fertilizer (10 g / kg) + soil conditioner of Example 3 (10 g / kg);

[0087] Test group 4: soil + ordinary organic fertilizer (10 g / kg) + soil conditioner of comparative example 1 (10 g / kg);

[0088] Test group 5: soil + common organic fertilizer (10 g / kg) + soil conditioner of comparative example 2 (10 g / kg);

[0089] Experiment 6: soil + common organic fertilizer (10 g / kg) + soil conditioner of comparative example 3 (10 g / kg);

[0090] Test group 7: soil + common organic fertilizer (10 g / kg) + soil conditioner of comparative example 4 (10 g / kg);

[0091] Each of the above groups was set up with 50 pots and repeated twice.

[0092] After the soil cultivation is completed, the Chinese cabbage seedlings with basically the same growth momentum are transplanted into seedling pots and harvested after 20 days of growth. 20 pots are randomly selected from each group to collect soil samples, and the soil pH and the content of organic matter, available phosphorus, available potassium, etc. are determined with reference to the "Soil Agricultural Chemical Analysis Methods"; 20 pots are randomly selected from each group to determine the growth characteristics of Chinese cabbage.

[0093] 2. Results and Analysis

[0094] 2.1 Effects of soil conditioners on soil properties

[0095] As can be seen from the results in Table 1, the application of the soil conditioner of the present invention can significantly increase the soil oH value, reduce soil acidity, and increase the content of exchangeable calcium and exchangeable magnesium in the soil, and the effect is better than using the soil conditioners of Comparative Examples 1-4.

[0096] Table 1 Effects of soil conditioners on soil physical and chemical properties

[0097] Group pH Exchangeable calcium (mg / kg) Exchangeable magnesium (mg / kg) Blank Group 5.08 525 71.3 Test Group 1 5.59 732 80.3 Test Group 2 5.71 701 78.6 Experiment 3 groups 5.68 695 81.5 Test 4 groups 5.13 594 73.9 Test 5 groups 5.15 568 72.5 Experiment 6 groups 5.11 572 76.3 Experiment 7 groups 5.12 549 75.1

[0098] Table 2 Effects of soil conditioners on soil nutrient content

[0099] Group Alkaline nitrogen (mg / kg) Available phosphorus (mg / kg) Available potassium (mg / kg) Organic matter (g / kg) Blank Group 131 85.9 135 20.3 Test Group 1 171 143.6 176 26.8 Test Group 2 173 129.5 183 25.1 Experiment 3 groups 168 136.8 169 25.9 Test 4 groups 145 98.3 159 22.3 Test 5 groups 133 96.7 141 21.2 Experiment 6 groups 138 101.2 153 21.8 Experiment 7 groups 142 93.5 146 22.6

[0100] Table 3 Effects of soil conditioners on soil heavy metal content

[0101] Group Chromium (mg / kg) Lead (mg / kg) Mercury (mg / kg) Arsenic (mg / kg) Blank Group 0.399 23.3 0.0126 0.245 Test Group 1 0.264 14.6 0.0072 0.164 Test Group 2 0.273 13.1 0.0051 0.179 Experiment 3 groups 0.268 15.8 0.0065 0.153 Test 4 groups 0.385 20.3 0.0105 0.227 Test 5 groups 0.381 21.1 0.0120 0.205 Experiment 6 groups 0.373 21.5 0.0113 0.223 Experiment 7 groups 0.378 20.9 0.0115 0.216

[0102] From the results in Table 2 and Table 3, it can be seen that after applying the soil conditioner of the present invention, the content of various nutrients in the soil can be significantly increased, and the content of nitrogen, phosphorus, potassium and organic matter can be increased; at the same time, the content of heavy metals in the soil can be effectively reduced.

[0103] 2.2 Effects of soil conditioners on the growth of Chinese cabbage

[0104] From the results in Table 4 below, it can be seen that after the soil was improved by the soil conditioner of the present invention, the overall growth of Chinese cabbage was better, the quality of the individual plants was higher, the leaf weight and leaf area were larger, and the petiole was shorter.

[0105] Table 4 Effects of soil conditioners on the growth of Chinese cabbage

[0106]

[0107] 2. Effects of soil conditioners on peanut growth

[0108] 1. Test and Methods

[0109] This experiment was conducted in an agricultural planting base in Linyi City. The soil properties were as follows: pH 4.98, organic matter 23.5g / kg, alkaline nitrogen 136.2mg / kg, available phosphorus 12.6mg / kg, available potassium 162.5mg / kg, exchangeable calcium 495.8mg / kg, exchangeable magnesium 70.3mg / kg, chromium 16.2mg / kg, cadmium 0.085mg / kg, lead 32.8mg / kg, mercury 0.026mg / kg, and arsenic 0.84mg / kg.

[0110] Set up 8 types of processing as follows:

[0111] Blank field: conventional fertilization;

[0112] Experimental field 1: conventional fertilization + soil conditioner of Example 1 (600 kg / hm 2 );

[0113] Experimental field 2: conventional fertilization + soil conditioner of Example 2 (600 kg / hm 2 );

[0114] Experimental field 3: conventional fertilization + soil conditioner of Example 3 (600 kg / hm 2 );

[0115] Experimental field 4: conventional fertilization + soil conditioner of comparative example 1 (600 kg / hm 2 );

[0116] Experimental field 5: conventional fertilization + soil conditioner of comparative example 2 (600 kg / hm 2 );

[0117] Experimental field 6: conventional fertilization + soil conditioner of comparative example 3 (600 kg / hm 2 );

[0118] Experimental field 7: conventional fertilization + soil conditioner of comparative example 4 (600 kg / hm 2 );

[0119] The area of ​​each treatment plot is 0.005hm 2 All treatments were applied as basal fertilizer once and turned into the ground during tillage. Peanuts were sown 15 hours later, with double rows and double seeds planted in ridges. The sowing density was 100,000 holes / hm2. 2 After planting, carry out field management according to normal management measures.

[0120] During the peanut harvest period, 5 soil samples were randomly collected in each plot using the "S" type sampling method to measure the soil properties. After the peanuts in each plot were harvested, the peanut traits such as the number of fruits per plant, the number of fruits per kilogram, and the rate of full fruits were examined.

[0121] 2. Results and Analysis

[0122] 2.1 Effects of soil conditioners on soil properties in peanut fields

[0123] Table 5 Effects of different soil conditioners on soil pH and chemical properties in peanut fields

[0124]

[0125]

[0126] Table 6 Effects of different soil conditioners on heavy metal content in peanut field soil

[0127] Group Chromium (mg / kg) Cadmium (mg / kg) Lead (mg / kg) Mercury (mg / kg) Arsenic (mg / kg) Blank Group 15.8 0.086 31.5 0.023 0.081 Experimental Field 1 13.2 0.053 26.4 0.009 0.057 Experimental Field 2 11.8 0.061 25.8 0.011 0.051 Experimental Field 3 12.5 0.059 27.3 0.013 0.046 Experimental Field 4 15.3 0.077 29.6 0.021 0.073 Experimental Field 5 14.9 0.081 28.1 0.016 0.069 Experimental Field 6 15.7 0.073 30.5 0.019 0.073 Experimental Field 7 15.1 0.076 28.9 0.015 0.065

[0128] From the results in Table 5 and Table 6 above, it can be seen that the soil conditioner of the present invention can effectively reduce soil acidity, increase the content of nutrients, and reduce the content of heavy metals.

[0129] 2.2 Effects of different soil conditioners on peanut traits

[0130] From the results in Table 7 below, it can be seen that the soil to which the conditioner of the present invention is applied is beneficial to the growth of peanuts, and the number of fruits per peanut plant, the rate of full fruits, and the yield are all significantly increased.

[0131] Table 7 Effects of different soil conditioners on peanut traits

[0132] Group Number of fruits per plant Fruit fullness rate (%) Number of kilograms of fruit <![CDATA[Yield (kg / hm 2 )]]> Blank Group 16.3 86.39 521.3 6123.14 Experimental Field 1 20.3 93.25 476.3 7352.48 Experimental Field 2 19.5 92.14 489.5 7252.36 Experimental Field 3 21.1 93.02 481.5 7302.05 Experimental Field 4 17.2 88.57 512.3 6859.74 Experimental Field 5 18.4 89.41 509.7 6714.52 Experimental Field 6 17.9 90.42 496.8 6802.59 Experimental Field 7 18.3 89.73 504.2 6635.48

Claims

1. An acidic soil conditioner, characterized in that: It is prepared from the following raw materials in parts by weight: 10-15 parts of Bacillus subtilis, 10-15 parts of EM bacteria, 20-30 parts of ethylhexylglycerol, 3-8 parts of chitin, 10-15 parts of sodium tripolyphosphate, 25-35 parts of calcium magnesium phosphate fertilizer, 12-15 parts of allantoin, 20-30 parts of modified oyster shells, and 35-45 parts of modified brewer's grains.

2. The acidic soil conditioner according to claim 1, characterized in that The effective viable count of the Bacillus subtilis is ≥ 20 billion / g, and the effective viable count of EM bacteria is ≥ 20 billion / g.

3. The acidic soil conditioner according to claim 1, characterized in that The preparation method of the modified oyster shell powder is: The oyster shell powder is soaked in a calcium hydroxide solution with a mass fraction of 10-15% for 10-12 hours, filtered, calcined in an oxygen-free environment at 700-800°C for 3-4 hours, then cooled to 300-400°C, sepiolite is added, kept warm for 1-1.5 hours, then cooled to 200-250°C, polyethylene glycol in a molten state is added, sodium carboxymethyl cellulose is added after mixing, mixed, cooled to room temperature, and crushed to obtain modified oyster shell powder.

4. The acidic soil conditioner according to claim 3, characterized in that In the modified oyster shell powder preparation method, the mass ratio of oyster shell powder, sepiolite, polyethylene glycol and sodium carboxymethyl cellulose is 1:(0.1-0.15):(0.8-1):(0.3-0.5).

5. The acidic soil conditioner according to claim 1, characterized in that The preparation method of the modified brewer's grains is: The brewer's grains are inoculated with an Aspergillus niger suspension at a solid-liquid ratio of 1:0.3 (g / mL), the pH is adjusted to 7.0-7.5, fermented and cultured at 25-30°C for 1-1.5 days, boiled with water, cooled to room temperature, adjusted to pH 5.0-6.0, cellulase, β-glucosidase, and Bacillus subtilis protease are added, enzymolysis is carried out at 35-40°C for 6-8 hours, boiled again, cooled to room temperature, and dried to obtain product I for standby use; 0.1 mol / L NaOH solution is added to product I at a solid-liquid ratio of 1:30 (g / mL), placed in a 40°C water bath for insulation for 3-4 hours, filtered, and a mixed solution containing triethanolamine and polyvinyl pyrrolidone is added at a solid-liquid ratio of 1:4 (g / mL), mixed well, and dried to obtain modified brewer's grains.

6. The acidic soil conditioner according to claim 5, characterized in that In the modified brewer's grains preparation method, the amount of Aspergillus niger spores in the Aspergillus niger suspension is 5×10 10 Pieces / mL.

7. The acidic soil conditioner according to claim 5, characterized in that In the modified brewer's grain preparation method, the added amount of cellulase is 5-8% of the brewer's grain mass, the added amount of beta-glucosidase is 4-7% of the brewer's grain mass, and the added amount of Bacillus subtilisin is 10-12% of the brewer's grain mass.

8. The acidic soil conditioner according to claim 5, characterized in that In the modified brewer's grains preparation method, the mass fraction of triethanolamine in the mixed solution is 10-15%, and the mass fraction of polyvinyl pyrrolidone is 25-30%.

9. A method for preparing the acidic soil conditioner according to any one of claims 1 to 8, characterized in that: The following steps are involved: Step A, mixing Bacillus subtilis, EM bacteria, chitin and ethylhexylglycerol, drying and crushing to obtain mixture I; Step B, mixing mixture I, sodium tripolyphosphate, calcium magnesium phosphate fertilizer, allantoin, modified oyster shell powder and modified brewer's grains to obtain an acidic soil conditioner.

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