Constant-temperature oscillator for vaccine shake-flask culture

Abstract

The invention belongs to the technical field of oscillators, particularly relates to a constant-temperature oscillator for vaccine shake-flask culture, and aims to solve the problem that the culture cost is greatly increased due to the fact that an existing shake-flask cannot be clamped and fixed and a corresponding water bath constant-temperature oscillator and a corresponding gas bath constant-temperature oscillator need to be used. An oscillation plate is arranged in the box body, a plurality of clamping components used for clamping shake flasks are arranged at the top of the oscillation plate, U-shaped handles are fixed to the two sides of the top of the oscillation plate and used for lifting the oscillation plate, and a temperature sensor is fixed to the inner wall of the bottom of the box body; the water tank is fixed to the bottom of the box body, air bath and water bath can be carried out on the shake flask on the same equipment, free switching of the water bath and the air bath can be completed through the switching structure, operation is easy, the cost of vaccine shake flask culture is greatly reduced, in addition, during oscillation, the water bath and the water bath can be separated, and the vaccine shake flask culture efficiency is improved. The shake flask can be clamped in all directions through cooperation of the clamping component, the first spring and the pressing plate.

Description

Technical Field

[0001] The invention relates to the technical field of oscillators, in particular to a constant temperature oscillator for vaccine shake flask culture. Background Art

[0002] Constant temperature oscillators for vaccine shake flask culture are widely used in biology, medicine, agricultural science and other fields, especially in vaccine production, cell culture, microbial fermentation, etc. In the process of vaccine culture, according to the vaccine to be cultured, the corresponding oscillator needs to be used, such as water bath constant temperature oscillator and air bath constant temperature oscillator.

[0003] In the prior art, the constant temperature oscillator still has the following disadvantages during use:

[0004] 1. When shaking the shake bottle containing the vaccine, the shake bottle is usually placed directly in the placement ring on the shaking plate. Since the shake bottle cannot be clamped and fixed, the shake bottle will shake greatly in the placement ring when the shaking plate is running, which may easily cause the shake bottle to break;

[0005] 2. When cultivating different vaccines, it is necessary to use corresponding water bath constant temperature oscillator and gas bath constant temperature oscillator. Therefore, when carrying out vaccine shake flask culture, it is necessary to prepare corresponding constant temperature oscillator, which greatly increases the cost of vaccine shake flask culture.

[0006] In view of the above problems, the present invention document proposes a constant temperature oscillator for vaccine shake flask culture. Summary of the invention

[0007] The purpose of the present invention is to solve the problem that the existing shake flask cannot be clamped and fixed, and the corresponding water bath constant temperature oscillator and air bath constant temperature oscillator need to be used, which greatly increases the culture cost, and proposes a constant temperature oscillator for vaccine shake flask culture.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] A constant temperature oscillator for vaccine shake flask culture, comprising a box body, an oscillating plate is arranged in the box body, a plurality of clamping members for clamping the shake flask are arranged on the top of the oscillating plate, U-shaped handles are fixed on both sides of the top of the oscillating plate for lifting the oscillating plate, and a temperature sensor is fixed on the bottom inner wall of the box body;

[0010] It also includes a water tank and a heating box, wherein the water tank is fixed at the bottom of the box body and is used to inject hot water into the box body for water bath heating, and the heating box is fixed at the top of the water tank and is fixedly connected to one side of the box body and is used to inject hot air into the box body for air bath heating;

[0011] It also includes a top cover arranged on the top of the box body, which is used to close the box body, and a sliding plate extending into the box body is slidably provided at the bottom of the top cover, and the sliding plate cooperates with the oscillating plate to clamp the shaking bottle from a vertical direction;

[0012] It also includes a rotating rod rotating in the box body, and the rotating rod is located below the oscillating plate;

[0013] The clamping structure is arranged in the sliding plate and is used to cooperate with the oscillating plate and the clamping member to clamp the shaking bottle in all directions;

[0014] The oscillating structure is arranged on one side of the box body and is used to drive the rotating rod to rotate to complete the reciprocating oscillating movement of the oscillating plate;

[0015] The water bath heating structure is arranged in the water tank and is used to inject hot water into the tank for water bath heating;

[0016] The air bath heating structure is arranged in the heating box and is used to inject hot air into the box for air bath heating, and the oscillating structure can drive the air bath heating structure to operate through the rotating rod;

[0017] The switching structure is arranged in the box body and is used to control the opening and closing of the gas bath heating structure, and the water bath heating structure can be used to drive the switching structure.

[0018] In a possible design, the clamping structure includes a plurality of sockets arranged at the bottom of the sliding plate, the top inner walls of the plurality of sockets are fixed with a first spring, the plurality of sockets are slidably connected with a pressing plate that contacts the top of the shaking bottle, the top of the pressing plate is fixedly connected to the bottom end of the first spring, and the elastic force of the first spring on the pressing plate can clamp the shaking bottle placed on the oscillating plate in a vertical direction;

[0019] The clamping member includes multiple vertical plates and clamping plates, the vertical plates are fixed on the top of the oscillation plate, the clamping plates are fixed on the top of the vertical plates, multiple spring sheets are fixed on the side of the clamping plate close to the shake bottle, the multiple spring sheets on the multiple clamping plates cooperate to clamp the shake bottle, and a rubber pad is provided on the side of the spring sheet close to the shake bottle for protecting the shake bottle; the shake bottle containing the vaccine is placed in the clamping member, the shake bottle is initially clamped by the cooperation of the vertical plates, the clamping plates and the spring sheets, the box body is closed by the top cover, the top of the shake bottle is just inserted into the socket and contacts the bottom of the pressure plate, and the elastic force of the first spring on the pressure plate can cooperate with the oscillation plate to vertically clamp the shake bottle, so the clamping member cooperates with the pressure plate and the oscillation plate to clamp and fix the shake bottle, thereby ensuring the stability of the shake bottle during later oscillation.

[0020] In a possible design, the oscillation structure includes a driving motor fixed to one side of the box body through a frame, the output shaft of the driving motor is fixedly connected to one end of a rotating rod through a coupling, the outer wall of the rotating rod is provided with a plurality of reciprocating threaded sections, the rotating rod is threadedly connected to a plurality of moving blocks through the plurality of reciprocating threaded sections, and the moving blocks slide on the bottom inner wall of the box body to drive the moving blocks to reciprocate, a plurality of return-shaped sleeves are fixed to the bottom of the oscillation plate, and the return-shaped sleeves are sleeved on the outer wall of the moving block to drive the oscillation plate to reciprocate and linearly move through the moving block, a plurality of guide blocks are fixed on both sides of the oscillation plate, and a plurality of first slideways are provided on the inner walls of the box body that are away from each other, and the guide blocks are slidably matched with the first slideways to be used When the oscillation plate is placed in the box, ensure that the moving block and the return-shaped sleeve are docked, and the inner walls of the box on both sides that are away from each other are provided with a second slide, and the second slide is slidably matched with the guide block, and the second slide is located below the first slide, and the bottoms of multiple first slides located on the same side are connected with the adjacent second slides, so as to facilitate the reciprocating movement of the oscillation plate in the later stage; when the oscillation plate is placed in the box along the first slide, the return-shaped sleeve is just put on the moving block, and the rotating rod drives the moving block to reciprocate through the reciprocating threaded section, and the cooperation of the moving block and the return-shaped sleeve drives the oscillation plate and the shaking bottle thereon to vibrate reciprocatingly. In addition, when the oscillation plate drives the shaking bottle to move, the top of the shaking bottle synchronously drives the sliding plate to move, so as to ensure the stability of the shaking bottle during the oscillation process.

[0021] In one possible design, the water bath heating structure includes a plurality of first heating plates fixed to the bottom inner wall of the water tank, which are used to heat the water in the water tank; a water pump is fixed to the bottom inner wall of the water tank through a frame; a liquid outlet pipe is fixed to the liquid outlet end of the water pump; the top end of the liquid outlet pipe is fixedly extended into the tank body, which is used to inject hot water into the tank body; a liquid return pipe is fixedly connected to the bottom of the tank body, and the bottom end of the liquid return pipe is fixedly extended into the water tank; the liquid outlet pipe and the liquid return pipe cooperate to circulate the hot water in the tank body and the water tank; the water in the water tank is heated by the first heating plates, and the water pump injects the hot water into the tank body through the liquid outlet pipe, and when the shaking bottle inside the tank body is heated by water bath, the solenoid valve on the liquid return pipe is opened to circulate the hot water in the tank body and the water tank, thereby ensuring the stability of the water temperature.

[0022] In one possible design, the air bath heating structure includes a mounting frame fixed in the heating box, a rotating shaft rotatably penetrates the mounting frame, a fan and a first bevel gear are fixed to the top and bottom ends of the rotating shaft respectively, a plurality of second heating plates located above the fan are fixed to the inner walls on both sides of the heating box away from each other, which are used to heat the air, an air outlet pipe is fixedly connected to the top of the heating box, and one end of the air outlet pipe is fixedly extended into the box body, which is used to inject the hot air in the heating box into the box body, a rotating shaft located below the first bevel gear is rotatably connected in the heating box, the outer wall of the rotating shaft is slidably connected to the second bevel gear through a slide groove and a slider, and the second bevel gear is meshed with the first bevel gear, and a side of the second bevel gear close to the box body is provided with An annular groove, one side of the box body is fixedly connected with an air return pipe, one end of the air return pipe is fixedly connected to the heating box, and the connection between the air return pipe and the heating box is located below the fan, and the end of the rotating rod away from the driving motor is sealed and rotated to extend into the heating box and is fixedly connected to one end of the rotating shaft, which is used to drive the second bevel gear to rotate; the air inside the heating box is heated by the second heating plate, and then the driving motor drives the rotating rod to rotate, and the rotating rod drives the second bevel gear and the fan to rotate through the rotating shaft, and the solenoid valves on the air outlet pipe and the air return pipe are opened, and the fan injects the hot air inside the heating box into the box body through the air outlet pipe, and the cold air in the box body flows back to the heating box through the air return pipe for heating again, thereby completing the air circulation and performing air bath heating on the shaking bottle.

[0023] In a possible design, the switching structure includes a plurality of vertical rods fixed to the inner wall of the bottom of the box body, and the vertical rods are located below the rotating rod. The outer wall sliding sleeves of the plurality of vertical rods are provided with the same floating plate, and the cross-section of the floating plate is trapezoidal, which is used to increase the contact area with water. The floating plate is rotatably connected to a connecting rod on one side close to the heating box, and the top of the connecting rod is rotatably connected to a push rod. One end of the push rod is sealed and slidable through one side of the inner wall of the box body and extends into the heating box in a sealed and slidable manner. One end of the push rod extends into the annular groove and slidably cooperates with the annular groove. The lifting and lowering of the floating plate is used to control the push rod and the second bevel gear The gear is moved to control the meshing and separation of the second bevel gear and the first bevel gear; the gear is injected into the box through the liquid outlet pipe. As the water level rises, the float moves up under the action of buoyancy, and the float pushes the push rod and the solenoid valve to move away from the box through the connecting rod, thereby releasing the meshing between the second bevel gear and the first bevel gear, thereby releasing the operation of the fan during a water bath; conversely, the float moves downward under the action of its own gravity and drives the connecting rod to rotate. The connecting rod pulls the solenoid valve to one side through the push rod, and when the rotating shaft drives the second bevel gear to rotate, it meshes with the first bevel gear, so that hot air can be injected into the box for an air bath.

[0024] In a possible design, limit plates are fixed on both sides of the bottom of the top cover, and the sides of the two limit plates that are away from each other are in contact with the inner wall of the corresponding side of the box body to increase the stability of the top cover closing the box body, and multiple sliding rods are fixed on the sides of the two limit plates that are close to each other, and one end of the multiple sliding rods slides and extends into the sliding plate, and multiple second springs are fixed between the two limit plates and the sliding plate, and the multiple second springs are respectively sleeved on the outer walls of the corresponding sliding rods to reset the sliding plate to the center position of the bottom of the top cover, so that when the top cover closes the box body, the top of the shaking bottle can be inserted into the socket.

[0025] In a possible design, a hemispherical rubber strip is fixed on the side of the two limit plates that are away from each other, and a card slot is provided on the inner walls of the box body that are close to each other. The hemispherical rubber strip and the card slot are mutually engaged, so as to ensure the stability of the top cover when the top cover closes the box body.

[0026] In a possible design, the outer walls of the air outlet pipe, the air return pipe and the liquid return pipe are all provided with solenoid valves.

[0027] In a possible design, a plurality of cavities are provided in the oscillation plate, a plurality of limit rods are slidably penetrated in the oscillation plate, and one end of the plurality of limit rods penetrates the corresponding cavities, a plurality of tops of the limit rods are provided with trapezoidal grooves, a plurality of top inner walls of the plurality of cavities are slidably penetrated by a plurality of pushing platforms, and the top end of the pushing platform slides and extends above the oscillation plate, and the plurality of pushing platforms are respectively located in a plurality of clamping members, a fixing column is fixed at the bottom of the plurality of pushing platforms, the bottom end of the fixing column is arc-shaped, and the bottom end of the fixing column cooperates with the trapezoidal groove to drive the pushing platform and the fixing column to reciprocate up and down, the two ends of the limit rod respectively conflict with the inner walls on both sides of the box body that are away from each other, and the bottom sides of the plurality of limit rods are provided with inclined surfaces, which are convenient for placing the oscillation plate and the limit rod in the box body; in the process of the moving block driving the oscillation plate to reciprocate and drive the shaking bottle to oscillate horizontally, the cooperation of the fixing column, the trapezoidal groove and the first spring can drive the shaking bottle to reciprocate longitudinally, thereby increasing the shaking bottle oscillation effect.

[0028] Beneficial effect: In the present invention, the top inner walls of the plurality of the jacks are all fixed with a first spring, the plurality of the jacks are all slidably connected with a pressure plate fixedly connected to the bottom end of the first spring, the clamping member is composed of a plurality of vertical plates and a clamping plate, the clamping plate is fixed to the top end of the vertical plate, and a plurality of spring sheets are fixed to the side of the clamping plate close to the shaking bottle; the shaking bottle placed in the clamping member is clamped by the cooperation of the vertical plate, the clamping plate and the spring sheet, the top cover closes the box body, the elastic force of the first spring on the pressure plate can cooperate with the oscillation plate to vertically clamp the shaking bottle, so that the shaking bottle can be fully clamped and fixed, ensuring the stability of the shaking bottle during the later oscillation;

[0029] In the present invention, the outer wall sliding sleeves of the plurality of vertical rods are provided with the same vertical rod, one side of the floating plate is rotatably connected with a connecting rod, the top end of the connecting rod is rotatably connected with a push rod, one end of the push rod seals and slides through one side inner wall of the box body and slidably cooperates with the annular groove; the buoyancy of the floating plate by water can control the left and right movement of the second bevel gear, and then when a water bath is carried out, the second bevel gear is disengaged from the first bevel gear, and vice versa, the second bevel gear is engaged with the first bevel gear, thereby completing the free switching between the water bath and the air bath, and the operation is simple;

[0030] In the present invention, a plurality of cavities are provided in the oscillation plate, a plurality of limit rods are slidably penetrated in the oscillation plate, a plurality of the limit rods are provided with trapezoidal grooves on the tops, a plurality of pushing platforms are slidably penetrated on the top inner walls of the plurality of cavities, and a plurality of fixed columns are fixed on the bottoms of the plurality of pushing platforms; in the process of the moving block driving the oscillation plate to move back and forth to drive the shaking bottle to oscillate horizontally, the cooperation of the fixed column, the trapezoidal groove and the first spring can drive the shaking bottle to move back and forth longitudinally, thereby increasing the shaking bottle oscillation effect.

[0031] In the present invention, the shake flask can be air-bathed and water-bathed on the same device, and the water bath and the air bath can be freely switched through the switching structure. The operation is simple and the cost of vaccine shake flask culture is greatly reduced. In addition, during oscillation, the shake flask can be clamped in all directions through the cooperation of the clamping component, the first spring and the pressure plate to ensure the stability of the shake flask. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 A schematic diagram of the three-dimensional structure of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0033] Figure 2 A schematic cross-sectional view of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0034] Figure 3 A schematic diagram of a three-dimensional cross-sectional structure of a box of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0035] Figure 4 A schematic diagram of a three-dimensional explosion structure of a top cover, a sliding plate, a pressing plate and a limiting plate of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0036] Figure 5 A schematic diagram of the three-dimensional structure of an oscillating plate and a clamping member of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0037] Figure 6 A schematic diagram of a three-dimensional exploded structure of a clamping component of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0038] Figure 7 A schematic diagram of a three-dimensional exploded cross-section structure of an oscillating plate and a moving block of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0039] Figure 8 A schematic diagram of a three-dimensional exploded cross-sectional structure of a heating box, a mounting frame and a rotating shaft of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0040] Fig. 9 A schematic diagram of a three-dimensional explosion structure of a floating plate, a connecting rod and a push rod of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0041] Fig.10 A schematic three-dimensional cross-sectional structure diagram of a box and an oscillation plate of a constant temperature oscillator for vaccine shake flask culture provided by the present invention;

[0042] Fig.11 The present invention provides a schematic cross-sectional structural diagram of an oscillating plate and a limiting rod of a constant temperature oscillator for vaccine shake flask culture.

[0043] In the figure: 1, box body; 2, oscillating plate; 3, clamping member; 4, vertical plate; 5, clamping plate; 6, spring sheet; 7, top cover; 8, sliding plate; 9, jack; 10, first spring; 11, pressure plate; 12, limit plate; 13, slide bar; 14, second spring; 15, hemispherical rubber strip; 16, card slot; 17, first slideway; 18, second slideway; 19, guide block; 20, rotating rod; 21, driving motor; 22, reciprocating thread section; 23, moving block; 24, return card sleeve; 25, water tank; 26, water pump; 27, outlet Liquid pipe; 28, temperature sensor; 29, first heating plate; 30, return liquid pipe; 31, heating box; 32, air outlet pipe; 33, return air pipe; 34, second heating plate; 35, mounting bracket; 36, rotating shaft; 37, fan; 38, first bevel gear; 39, rotating shaft; 40, second bevel gear; 41, push rod; 42, vertical rod; 43, floating plate; 44, connecting rod; 45, limit rod; 46, cavity; 47, pushing platform; 48, fixing column; 49, trapezoidal groove; 50, solenoid valve; 51, U-shaped handle; 52, annular groove. DETAILED DESCRIPTION

[0044] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0045] Example 1: Reference Figure 1 , Figure 2 and Figure 5, an oscillator, relates to the technical field of oscillators, and the constant temperature oscillator comprises a box body 1, and an oscillating plate 2 is arranged inside the box body 1. A plurality of clamping members 3 are designed on the top of the oscillating plate 2, and these clamping members 3 are used to firmly clamp the vaccine shake bottle. In addition, U-shaped handles 51 are fixed on both sides of the top of the oscillating plate 2, and these handles allow the user to conveniently lift the oscillating plate 2 to facilitate loading or unloading of the vaccine shake bottle.

[0046] Reference Figure 1 A temperature sensor 28 is fixed on the bottom inner wall of the box 1 for real-time monitoring of the temperature inside the box 1.

[0047] In order to provide two heating methods, water bath heating and gas bath heating, we designed the following structure:

[0048] Reference Figure 1 and Figure 2 The water tank 25 is fixed at the bottom of the box body 1, and its function is to inject hot water into the box body 1 for water bath heating. The heating box 31 is fixed at the top of the water tank 25, and the heating box 31 is fixedly connected to one side of the box body 1. The function of the heating box 31 is to inject hot air into the box body 1 for air bath heating.

[0049] Reference Figure 1 and Figure 2 In order to close the box body 1, a top cover 7 is provided on the top thereof. A sliding plate 8 is slidably connected to the bottom of the top cover 7, and the sliding plate 8 can extend into the inside of the box body 1 and cooperate with the oscillating plate 2 to clamp the vaccine shake bottle from the vertical direction.

[0050] Reference Figure 1 A rotating rod 20 is rotatably arranged inside the box body 1 , and the rotating rod 20 is located below the oscillating plate 2 .

[0051] Reference Figure 4 , both sides of the bottom of the top cover 7 are fixed with limit plates 12, which are respectively located on the left and right sides of the bottom of the top cover 7. The sides of the two limit plates 12 that are away from each other, that is, their outer sides, are tightly fitted with the inner wall of the corresponding side of the box body 1. This design increases the stability of the top cover 7 when closing the box body 1, and prevents the top cover 7 from shaking or misaligning during the closing process.

[0052] Reference Figure 4At the same time, a plurality of slide bars 13 are fixed to the side where the two limit plates 12 are close to each other, that is, their inner sides. One end of these slide bars 13 extends into the sliding plate 8 in a sliding manner, so that the sliding plate 8 can slide on the slide bars 13. In order to maintain the stability of the sliding plate 8 at the center position of the bottom of the top cover 7, a plurality of second springs 14 are fixed between the two limit plates 12 and the sliding plate 8. These second springs 14 are respectively sleeved on the outer walls of the corresponding slide bars 13. When the sliding plate 8 is deviated from the center position by external force, the second springs 14 will provide elastic force to reset the sliding plate 8 to the center position of the bottom of the top cover 7. In this way, when the top cover 7 closes the box body 1, the top end of the shaking bottle can be more easily inserted into the socket 9.

[0053] Reference Figure 3 and Figure 4 A hemispherical rubber strip 15 is fixed to the side of the two limit plates 12 that are away from each other. These hemispherical rubber strips 15 have a certain elasticity and friction force, and can be engaged with the slots 16 on the inner walls of the two sides close to each other of the box body 1. When the top cover 7 closes the box body 1, the hemispherical rubber strips 15 will be inserted into the slots 16, thereby increasing the stability and sealing between the top cover 7 and the box body 1.

[0054] Next, we describe the clamping structure in detail:

[0055] Reference Figure 4-Figure 6 The bottom of the sliding plate 8 is designed with a plurality of insertion holes 9, and a first spring 10 is fixed to the top inner wall of each insertion hole 9. A pressing plate 11 is slidably connected in each insertion hole 9, and the top of the pressing plate 11 is fixedly connected to the bottom end of the first spring 10. When the vaccine shake bottle is placed on the oscillating plate 2, the elastic force of the first spring 10 on the pressing plate 11 can firmly clamp the vaccine shake bottle from the vertical direction.

[0056] Reference Figure 5 and Figure 6 The clamping member 3 includes a plurality of vertical plates 4 and a clamping plate 5. The vertical plates 4 are fixed to the top of the oscillating plate 2, and the clamping plate 5 is fixed to the top of the vertical plates 4. A plurality of spring sheets 6 are fixed to the side of the clamping plate 5 close to the vaccine shake bottle, and these spring sheets 6 cooperate with each other to clamp the vaccine shake bottle. A rubber pad is also provided on the side of the spring sheet 6 close to the vaccine shake bottle to increase the stability of the clamping and the protection of the vaccine shake bottle.

[0057] Next, we describe the oscillatory structure:

[0058] Reference Figure 2 and Figure 7, a driving motor 21 is fixed to one side of the box body 1 through a frame, and its output shaft is fixedly connected to one end of the rotating rod 20 through a coupling. The outer wall of the rotating rod 20 is designed with multiple reciprocating thread segments 22, and these reciprocating thread segments 22 are threadedly connected to multiple moving blocks 23. These moving blocks 23 can slide on the bottom inner wall of the box body 1 to drive them to reciprocate. A plurality of return-shaped ferrules 24 are fixed to the bottom of the oscillation plate 2, and these return-shaped ferrules 24 are sleeved on the outer wall of the moving blocks 23. When the moving blocks 23 reciprocate, they will drive the oscillation plate 2 to reciprocate linearly through the return-shaped ferrules 24.

[0059] Reference Figure 3 and Figure 7 In order to ensure the stability of the oscillation plate 2 in the box body 1, we set a plurality of first slideways 17 and second slideways 18 on the inner walls of the box body 1 that are far away from each other. A plurality of guide blocks 19 are fixed on both sides of the oscillation plate 2, and these guide blocks 19 can slide with the first slideways 17 and the second slideways 18. When the oscillation plate 2 is placed in the box body 1 along the first slideway 17, the return sleeve 24 will just fit on the moving block 23. In addition, the bottoms of the plurality of first slideways 17 on the same side are connected to the adjacent second slideways 18 to facilitate the reciprocating movement of the oscillation plate 2 in the later stage.

[0060] Reference Figure 2 and Figure 8 When the rotating rod 20 drives the oscillating plate 2 to move back and forth, it can also drive the air bath heating structure to operate. The specific implementation of the water bath heating structure is as follows:

[0061] The specific implementation of the gas bath heating structure is as follows:

[0062] Reference Figure 2 and Figure 8A heating box 31 is provided on one side of the housing 1. A mounting frame 35 is fixed in the heating box 31 by screws. A rotating shaft 36 is rotatably passed through the mounting frame 35. A fan 37 and a first bevel gear 38 are fixed to the top and bottom of the rotating shaft 36 by screws respectively. A plurality of second heating plates 34 located above the fan 37 are fixed to the inner walls of the heating box 31 on both sides away from each other by screws, which are used to heat the air. An air outlet pipe 32 is fixedly connected to the top of the heating box 31, and one end of the air outlet pipe 32 is fixedly extended into the housing 1 by a flange, which is used to inject the hot air in the heating box 31 into the housing 1. A rotating shaft 39 located below the first bevel gear 38 is rotatably connected in the heating box 31. A second bevel gear 40 is slidably connected to the outer wall of the rotating shaft 39 by a sliding groove and a slider, and the second bevel gear 40 is meshed with the first bevel gear 38. An annular groove 52 is provided on the side of the second bevel gear 40 close to the housing 1. One side of the box body 1 is fixedly connected to a return air pipe 33 via a flange, one end of the return air pipe 33 is fixedly connected to the heating box 31 via a flange, and the connection between the return air pipe 33 and the heating box 31 is located below the fan 37.

[0063] Specifically, the rotating rod 20 of the driving motor 21 of the oscillator is sealed and rotated away from one end of the driving motor 21, extending into the heating box 31 and fixedly connected to one end of the rotating shaft 39 through a coupling, so as to drive the second bevel gear 40 and the fan 37 to rotate. When it is necessary to perform air bath heating on the shaking flask inside the box 1, the air inside the heating box 31 is first heated by the second heating plate 34, and then the driving motor 21 drives the rotating rod 20 to rotate, and the rotating rod 20 drives the second bevel gear 40 and the fan 37 to rotate through the rotating shaft 39, and at the same time, the electromagnetic valve 50 on the air outlet pipe 32 and the air return pipe 33 is opened, and the fan 37 injects the hot air inside the heating box 31 into the box 1 through the air outlet pipe 32, and the cold air in the box 1 flows back to the heating box 31 through the air return pipe 33 to be heated again, thereby completing the air circulation and performing air bath heating on the shaking flask.

[0064] Reference Figure 2 The water bath structure includes a water tank 25 arranged at the bottom of the box body 1, and a plurality of first heating plates 29 are fixed to the bottom inner wall of the water tank 25 by screws, which are used to heat the water in the water tank 25. A water pump 26 is also fixed to the bottom inner wall of the water tank 25 through a frame, and a liquid outlet pipe 27 is connected to the liquid outlet end of the water pump 26 through a flange, and the top of the liquid outlet pipe 27 is fixed to the box body 1 through a flange, which is used to inject hot water into the box body 1. A liquid return pipe 30 is fixed to the bottom of the box body 1 through a flange, and the bottom end of the liquid return pipe 30 is fixed to the water tank 25 through a flange. Solenoid valves 50 are provided on both the liquid outlet pipe 27 and the liquid return pipe 30, and the hot water in the box body 1 and the water tank 25 can circulate through the control of the solenoid valve 50.

[0065] Specifically, when it is necessary to heat the shaking bottle inside the housing 1 in a water bath, the water in the water tank 25 is first heated by the first heating plate 29, and then the water pump 26 injects the hot water into the housing 1 through the liquid outlet pipe 27. At the same time, the solenoid valve 50 on the liquid return pipe 30 is opened to circulate the hot water in the housing 1 and the water tank 25 to ensure the stability of the water temperature.

[0066] In the design of the constant temperature oscillator, electromagnetic valves 50 are provided on the outer walls of the gas outlet pipe 32, the gas return pipe 33 and the liquid return pipe 30. These electromagnetic valves 50 can control the opening and closing of the corresponding pipes, thereby realizing precise control of gas and liquid. For example, when it is necessary to adjust the gas composition or pressure in the incubator, it can be achieved by opening or closing the corresponding electromagnetic valve 50.

[0067] Reference Figure 2 , Figure 8 and Fig. 9 , and the water bath heating structure can drive a switching structure, which is used to control the opening and closing of the gas bath heating structure. The specific implementation of the switching structure is as follows:

[0068] Reference Figure 8 and Fig. 9 , a plurality of vertical rods 42 are fixed to the inner wall of the bottom of the box body 1 by screws, and the vertical rods 42 are located below the rotating rod 20. A floating plate 43 is provided on the sliding sleeve of the outer wall of the plurality of vertical rods 42, and the cross section of the floating plate 43 is trapezoidal, which is used to increase the contact area with water. The side of the floating plate 43 close to the heating box 31 is rotatably connected to a connecting rod 44 through a pin shaft, and the top of the connecting rod 44 is rotatably connected to a push rod 41 through a pin shaft. One end of the push rod 41 slides through the inner wall of one side of the box body 1 in a sealed manner and extends into the heating box 31 in a sealed manner. One end of the push rod 41 extends into the annular groove 52 and slides with the annular groove 52. The lifting and lowering of the floating plate 43 can control the movement of the push rod 41 and the second bevel gear 40, thereby controlling the meshing and separation of the second bevel gear 40 and the first bevel gear 38.

[0069] Specifically, when hot water is injected into the housing 1 through the liquid outlet pipe 27, as the water level rises, the floating plate 43 moves upward under the action of buoyancy, and the floating plate 43 pushes the push rod 41 and the electromagnetic valve 50 to move away from the housing 1 through the connecting rod 44. The push rod 41 drives the second bevel gear 40 to move through the annular groove 52, and the meshing between the second bevel gear 40 and the first bevel gear 38 is released, so that the operation of the fan 37 can be released during the water bath. Conversely, when the water level in the housing 1 drops, the floating plate 43 moves downward under the action of its own gravity and drives the connecting rod 44 to rotate. The connecting rod 44 pulls the electromagnetic valve 50 to one side through the push rod 41. At this time, the second bevel gear 40 is driven by the rotating shaft 39 to rotate and mesh with the first bevel gear 38, so that hot air can be injected into the housing 1 for air bathing.

[0070] This constant temperature oscillator for vaccine shake flask culture has a reasonable design and compact structure. It can firmly clamp the vaccine shake flask and ensure its stability during the oscillation process. At the same time, by providing two heating methods, users can choose the appropriate heating method according to actual needs to meet different culture requirements.

[0071] Example 2: Reference Fig.10 and Fig.11 , based on the improvement of embodiment 1: a plurality of cavities 46 are provided in the oscillating plate 2, and these cavities 46 are used to accommodate and support the limiting rods 45. A plurality of limiting rods 45 are also slidably penetrated in the oscillating plate 2, and one end of these limiting rods 45 penetrates the corresponding cavities 46. A trapezoidal groove 49 is provided on the top of the limiting rod 45, and these trapezoidal grooves 49 are used to cooperate with the fixing column 48.

[0072] refer to Fig.11 At the same time, multiple push platforms 47 are slidably penetrated through the top inner walls of the multiple cavities 46, and the top ends of these push platforms 47 slide and extend above the oscillation plate 2 and are respectively located in multiple clamping members 3. Fixed columns 48 are fixed to the bottom of the push platforms 47, and the bottom ends of these fixed columns 48 are arc-shaped and can be matched with the trapezoidal grooves 49.

[0073] refer to Fig.11 In the process of the moving block 23 driving the oscillation plate 2 to reciprocate and drive the shake bottle to oscillate horizontally, the fixed column 48 will slide in the trapezoidal groove 49. Due to the shape of the trapezoidal groove 49 and the arc-shaped bottom design of the fixed column 48, the fixed column 48 will be guided by the trapezoidal groove 49 to reciprocate up and down during the sliding process. This up and down reciprocating movement will drive the pusher 47 to move together, thereby realizing the longitudinal reciprocating movement drive of the shake bottle.

[0074] refer to Fig.10 and Fig.11 In addition, the two ends of the limit rod 45 respectively contact the inner walls of the box 1 that are away from each other, which can limit the movement range of the oscillation plate 2 during the lateral oscillation process. At the same time, both sides of the bottom of the limit rod 45 are provided with inclined surfaces, which can facilitate the placement of the oscillation plate 2 and the limit rod 45 in the box 1.

[0075] With this design, when the moving block 23 drives the oscillation plate 2 to reciprocate, the shake flask is not only subjected to the lateral oscillation force, but also to the longitudinal reciprocating force generated by the cooperation of the fixed column 48, the trapezoidal groove 49 and the first spring 10. This composite oscillation mode can more effectively promote the growth and reproduction of cells or microorganisms in the shake flask, and improve the production efficiency and quality of vaccines.

[0076] A method for using a constant temperature oscillator for vaccine shake flask culture, comprising the following steps:

[0077] S1. Before use, place the shake bottle containing the vaccine in the clamping member 3, and initially clamp the shake bottle through the cooperation of the vertical plate 4, the clamping plate 5 and the spring sheet 6, and then place the oscillating plate 2 in the box body 1 through the U-shaped handle 51. When placing, the guide block 19 and the first slide 17 are slidably matched to place it in the box body 1 until the guide block 19 moves into the second slide 18, completing the initial placement of the oscillating plate 2 and the shake bottle;

[0078] S2, then the box body 1 is closed by the top cover 7, and the engagement of the hemispherical rubber strip 15 and the card slot 16 can fix the top cover 7, and when the top cover 7 closes the box body 1, the top of the shaking bottle is just inserted into the insertion hole 9 and contacts the bottom of the pressing plate 11, and the elastic force of the first spring 10 on the pressing plate 11 can cooperate with the oscillation plate 2 to clamp the shaking bottle vertically, so the clamping member 3 cooperates with the pressing plate 11 and the oscillation plate 2 to clamp and fix the shaking bottle, thereby ensuring the stability of the shaking bottle during the later oscillation;

[0079] S3, when the shaking bottle needs to be heated and oscillated by air bath, the air inside the heating box 31 is heated by the second heating plate 34, and then the driving motor 21 drives the rotating rod 20 to rotate, and the rotating rod 20 drives the rotating shaft 39 to rotate, and the floating plate 43 moves downward under the action of its own gravity and drives the connecting rod 44 to rotate, and the connecting rod 44 pulls the electromagnetic valve 50 to one side through the push rod 41, and when the rotating shaft 39 drives the second bevel gear 40 to rotate, it meshes with the first bevel gear 38, so that the rotating shaft 39 drives the fan 37 to rotate through the cooperation of the second bevel gear 40 and the first bevel gear 38, and then the electromagnetic valve 50 on the air outlet pipe 32 and the return air pipe 33 is opened, and the fan 37 injects the hot air inside the heating box 31 into the box body 1 through the air outlet pipe 32 to control the temperature inside the box body 1, and the temperature sensor 28 can detect the stability inside the box body 1 in real time, and the cold air in the box body 1 flows back to the heating box 31 through the return air pipe 33 to be heated again, thereby completing the air circulation and performing air bath heating on the shaking bottle;

[0080] S4, when the oscillating plate 2 is placed into the box body 1 along the first slide 17, the return sleeve 24 is just sleeved on the moving block 23, so during oscillation, the rotating rod 20 drives the moving block 23 to move back and forth through the reciprocating threaded section 22, and the cooperation between the moving block 23 and the return sleeve 24 drives the oscillating plate 2 and the shaking bottle thereon to vibrate back and forth. In addition, when the oscillating plate 2 drives the shaking bottle to move, the top of the shaking bottle synchronously drives the sliding plate 8 to move, thereby ensuring the stability of the shaking bottle during oscillation;

[0081] S5. When the shaking bottle needs to be heated by water bath, the water in the water tank 25 is heated by the first heating plate 29, and the water pump 26 injects the hot water into the box body 1 through the liquid outlet pipe 27. As the water level rises, the floating plate 43 moves upward under the action of buoyancy, and the floating plate 43 pushes the push rod 41 and the electromagnetic valve 50 to move away from the box body 1 through the connecting rod 44, releasing the meshing between the second bevel gear 40 and the first bevel gear 38. Therefore, when the rotating rod 20 rotates to drive the oscillation plate 2 to oscillate in the later stage, the fan 37 will not run idle, and when the shaking bottle inside the box body 1 is heated by water bath, the electromagnetic valve 50 on the liquid return pipe 30 is opened to circulate the hot water in the box body 1 and the water tank 25 to ensure the stability of the water temperature.

[0082] S6. When the moving block 23 drives the oscillation plate 2 to move back and forth to drive the shake bottle to oscillate horizontally, the cooperation between the fixed column 48, the trapezoidal groove 49 and the first spring 10 can drive the shake bottle to move back and forth longitudinally, thereby increasing the shaking effect of the shake bottle.

[0083] However, as is well known to those skilled in the art, the working principles and wiring methods of the second heating plate 34, the solenoid valve 50, the water pump 26, the first heating plate 29, the drive motor 21 and the temperature sensor 28 are commonplace, and are conventional means or common knowledge, and will not be elaborated here. Those skilled in the art may make any optional selections according to their needs or convenience.

[0084] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A constant temperature oscillator for vaccine shake flask culture, characterized in that: The invention comprises a box body (1), wherein an oscillating plate (2) is arranged inside the box body (1), a plurality of clamping members (3) for clamping a shaking bottle are arranged on the top of the oscillating plate (2), U-shaped handles (51) are fixed on both sides of the top of the oscillating plate (2) for lifting the oscillating plate (2), and a temperature sensor (28) is fixed on the inner wall of the bottom of the box body (1); It also comprises a water tank (25) and a heating box (31), wherein the water tank (25) is fixed to the bottom of the box body (1) and is used to inject hot water into the box body (1) for water bath heating, and the heating box (31) is fixed to the top of the water tank (25), and the heating box (31) is fixedly connected to one side of the box body (1) and is used to inject hot air into the box body (1) for air bath heating; It also includes a top cover (7) arranged on the top of the box body (1) and used to close the box body (1); a sliding plate (8) is slidably provided at the bottom of the top cover (7) and extends into the box body (1); and the sliding plate (8) cooperates with the oscillating plate (2) to clamp the shaking bottle in a vertical direction; It also includes a rotating rod (20) rotating in the box body (1), and the rotating rod (20) is located below the oscillating plate (2); A clamping structure is arranged inside the sliding plate (8) and is used to cooperate with the oscillating plate (2) and the clamping member (3) to clamp the shaking bottle in all directions; An oscillating structure, arranged on one side of the box body (1), used to drive the rotating rod (20) to rotate to complete the reciprocating oscillating movement of the oscillating plate (2); A water bath heating structure is arranged in the water tank (25) and is used to inject hot water into the tank body (1) for water bath heating; An air bath heating structure is arranged in the heating box (31) and is used to inject hot air into the box body (1) for air bath heating, and the oscillating structure can drive the air bath heating structure to operate through the rotating rod (20); The switching structure is arranged in the box (1) and is used to control the opening and closing of the gas bath heating structure, and the water bath heating structure can be used to drive the switching structure.

2. A constant temperature oscillator for vaccine shake flask culture according to claim 1, characterized in that: The clamping structure comprises a plurality of insertion holes (9) arranged at the bottom of the sliding plate (8), the top inner walls of the plurality of insertion holes (9) are all fixed with first springs (10), the plurality of insertion holes (9) are all slidably connected with a pressing plate (11) that contacts the top of the shaking bottle, the top of the pressing plate (11) is fixedly connected to the bottom of the first spring (10), and the elastic force of the first spring (10) on the pressing plate (11) can clamp the shaking bottle placed on the oscillating plate (2) in a vertical direction; The clamping member (3) comprises a plurality of vertical plates (4) and a clamping plate (5), wherein the vertical plates (4) are fixed to the top of the oscillating plate (2), and the clamping plate (5) is fixed to the top of the vertical plates (4). A plurality of spring sheets (6) are fixed to the side of the clamping plate (5) close to the shaking bottle, and the plurality of spring sheets (6) on the plurality of clamping plates (5) cooperate to clamp the shaking bottle, and a rubber pad is provided on the side of the spring sheet (6) close to the shaking bottle for protecting the shaking bottle.

3. A constant temperature oscillator for vaccine shake flask culture according to claim 1, characterized in that: The oscillation structure comprises a driving motor (21) fixed to one side of a housing (1) via a frame, an output shaft of the driving motor (21) being fixedly connected to one end of a rotating rod (20) via a coupling, a plurality of reciprocating threaded sections (22) being provided on the outer wall of the rotating rod (20), a plurality of moving blocks (23) being threadedly connected to the rotating rod (20) via the plurality of reciprocating threaded sections (22), and the moving blocks (23) sliding on the bottom inner wall of the housing (1) for driving the moving blocks (23) to reciprocate, a plurality of return sleeves (24) being fixed to the bottom of the oscillation plate (2), and the return sleeves (24) being sleeved on the outer wall of the moving blocks (23) for reciprocating movement through the moving blocks The moving block (23) drives the oscillating plate (2) to move back and forth in a straight line. A plurality of guide blocks (19) are fixed on both sides of the oscillating plate (2). The inner walls on both sides of the box body (1) that are away from each other are provided with a plurality of first slideways (17), and the guide blocks (19) are slidably matched with the first slideways (17). The inner walls on both sides of the box body (1) that are away from each other are provided with second slideways (18), and the second slideways (18) are slidably matched with the guide blocks (19). The second slideways (18) are located below the first slideways (17), and the bottoms of the plurality of first slideways (17) located on the same side are connected to the adjacent second slideways (18), so as to facilitate the reciprocating movement of the oscillating plate (2) in the later stage.

4. A constant temperature oscillator for vaccine shake flask culture according to claim 1, characterized in that: The water bath heating structure comprises a plurality of first heating plates (29) fixed to the inner wall of the bottom of the water tank (25) and used for heating the water in the water tank (25). A water pump (26) is fixed to the inner wall of the bottom of the water tank (25) through a frame. A liquid outlet pipe (27) is fixed to the liquid outlet end of the water pump (26). The top end of the liquid outlet pipe (27) is fixedly extended into the box body (1) and used for injecting hot water into the box body (1). A liquid return pipe (30) is fixedly connected to the bottom of the box body (1). The bottom end of the liquid return pipe (30) is fixedly extended into the water tank (25). The liquid outlet pipe (27) cooperates with the liquid return pipe (30) to circulate the hot water in the box body (1) and the water tank (25).

5. A constant temperature oscillator for vaccine shake flask culture according to claim 4, characterized in that: The air bath heating structure comprises a mounting frame (35) fixed in the heating box (31), a rotating shaft (36) rotatably passing through the mounting frame (35), a fan (37) and a first bevel gear (38) being fixed to the top and bottom ends of the rotating shaft (36), respectively, a plurality of second heating plates (34) located above the fan (37) are fixed to inner walls on both sides of the heating box (31) away from each other, for heating air, an air outlet pipe (32) is fixedly connected to the top of the heating box (31), and one end of the air outlet pipe (32) is fixedly extended into the box body (1), for injecting hot air in the heating box (31) into the box body (1), and a rotating gear (37) located below the first bevel gear (38) is rotatably connected to the heating box (31). A shaft (39) is provided, the outer wall of the rotating shaft (39) is slidably connected with a second bevel gear (40) through a sliding groove and a sliding block, and the second bevel gear (40) is meshed with the first bevel gear (38), a side of the second bevel gear (40) close to the housing (1) is provided with an annular groove (52), one side of the housing (1) is fixedly connected with a return air pipe (33), one end of the return air pipe (33) is fixedly connected with the heating box (31), and the connection between the return air pipe (33) and the heating box (31) is located below the fan (37), and one end of the rotating rod (20) away from the driving motor (21) is sealed and rotatably extended into the heating box (31) and is fixedly connected with one end of the rotating shaft (39) for driving the second bevel gear (40) to rotate.

6. A constant temperature oscillator for vaccine shake flask culture according to claim 5, characterized in that: The switching structure comprises a plurality of vertical rods (42) fixed to the inner wall at the bottom of the box body (1), and the vertical rods (42) are located below the rotating rod (20); the outer walls of the plurality of vertical rods (42) are slidingly sleeved with a same floating plate (43), and the cross section of the floating plate (43) is trapezoidal, which is used to increase the contact area with water; the floating plate (43) is rotatably connected to a connecting rod (44) on one side close to the heating box (31); the top end of the connecting rod (44) is rotatably connected to a push rod (41); one end of the push rod (41) seals and slides through the inner wall of one side of the box body (1) and seals and slides and extends into the heating box (31); one end of the push rod (41) extends into the annular groove (52) and slidably cooperates with the annular groove (52); the lifting and lowering of the floating plate (43) is used to control the movement of the push rod (41) and the second bevel gear (40), thereby controlling the meshing and separation of the second bevel gear (40) and the first bevel gear (38).

7. A constant temperature oscillator for vaccine shake flask culture according to claim 2, characterized in that: Limiting plates (12) are fixed on both sides of the bottom of the top cover (7), and the sides of the two limiting plates (12) that are away from each other are in contact with the inner wall of the corresponding side of the box body (1) to increase the stability of the top cover (7) closing the box body (1). Multiple sliding rods (13) are fixed on the sides of the two limiting plates (12) that are close to each other, and one end of the multiple sliding rods (13) slides and extends into the sliding plate (8). Multiple second springs (14) are fixed between the two limiting plates (12) and the sliding plate (8), and the multiple second springs (14) are respectively sleeved on the outer walls of the corresponding sliding rods (13) to reset the sliding plate (8) to the center position of the bottom of the top cover (7).

8. A constant temperature oscillator for vaccine shake flask culture according to claim 7, characterized in that: A hemispherical rubber strip (15) is fixed on the side of the two limit plates (12) that are away from each other, and a clamping groove (16) is provided on the inner walls of the box body (1) that are close to each other, and the hemispherical rubber strip (15) and the clamping groove (16) are clamped with each other.

9. A constant temperature oscillator for vaccine shake flask culture according to claim 5, characterized in that: The outer walls of the air outlet pipe (32), the air return pipe (33) and the liquid return pipe (30) are all provided with electromagnetic valves (50).

10. A constant temperature oscillator for vaccine shake flask culture according to claim 3, characterized in that: The oscillating plate (2) is provided with a plurality of cavities (46), the oscillating plate (2) is slidably penetrated by a plurality of limiting rods (45), one end of each of the limiting rods (45) penetrates the corresponding cavity (46), the tops of each of the limiting rods (45) are provided with a trapezoidal groove (49), the top inner walls of each of the cavities (46) are slidably penetrated by a plurality of pushing platforms (47), the tops of the pushing platforms (47) are slidably extended to the top of the oscillating plate (2), and the plurality of pushing platforms (47) are respectively located on the plurality of clamping members (3) A fixing column (48) is fixed to the bottom of each of the plurality of pushing platforms (47), the bottom end of each of the fixing columns (48) is arc-shaped, and the bottom end of each of the fixing columns (48) cooperates with a trapezoidal groove (49) to drive the pushing platform (47) and the fixing columns (48) to reciprocate up and down. The two ends of the limiting rod (45) respectively contact the inner walls of the box body (1) on both sides that are away from each other. Inclined surfaces are provided on both sides of the bottom of each of the plurality of limiting rods (45) to facilitate placing the oscillating plate (2) and the limiting rod (45) in the box body (1).

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