Preparation method of microbial accelerant for treating total nitrogen in sewage

Abstract

The invention relates to a preparation method of a microbial promoter for treating total nitrogen in sewage, the method can obviously improve the removal efficiency of total nitrogen in sewage, and compared with a traditional biological method and a traditional physical and chemical method, the method has the advantages that the treatment speed is higher, and an ideal purification effect can be more quickly achieved. The microbial accelerant is simple and convenient to prepare and low in cost, the use of chemical agents is reduced, and the sewage treatment cost is effectively reduced. Besides, the total nitrogen in the sewage is treated in a microbial natural degradation manner, so that secondary pollution possibly caused by a traditional chemical treatment method is avoided, and a more environment-friendly treatment target is achieved. Particularly, the specially domesticated microorganisms have good environmental adaptability, and can still maintain efficient activity even if environmental factors such as temperature, pH value or dissolved oxygen fluctuate, so that the stability and reliability of the treatment effect are ensured.

Description

Technical Field

[0001] The invention relates to the technical field of sewage treatment, and in particular to a method for preparing a microbial promoter for treating total nitrogen in sewage. Background Art

[0002] With the acceleration of industrialization and urbanization, water pollution is becoming increasingly serious, among which total nitrogen (TN) pollution is particularly prominent. Total nitrogen includes various forms such as ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and organic nitrogen, and is one of the main causes of eutrophication of water bodies. Although traditional sewage treatment methods such as physical and chemical methods and biological methods can remove total nitrogen to a certain extent, they have problems such as high cost, high energy consumption and secondary pollution. Therefore, it is particularly important to develop efficient, economical and environmentally friendly total nitrogen removal technology. In recent years, microbial technology has been widely used in the field of sewage treatment due to its high efficiency, low cost and environmental friendliness. However, existing microbial treatment technologies still face some challenges in practical applications and need further improvement and optimization.

[0003] At present, the methods for microbial treatment of total nitrogen in sewage mainly include biological denitrification processes, such as anaerobic ammonia oxidation and denitrification. Although these methods have shown good results under laboratory conditions, there are still some problems in practical applications. First, the activity of microorganisms is greatly affected by environmental factors, such as temperature, pH value, dissolved oxygen, etc. Changes in these factors will significantly affect the growth and metabolism of microorganisms, thereby affecting the removal efficiency of total nitrogen. Secondly, toxic and harmful substances in sewage (such as heavy metals and organic pollutants) will inhibit microorganisms, reducing their activity and treatment capacity. In addition, the reproduction rate of microorganisms is slow, resulting in a long treatment cycle, which is difficult to meet the needs of large-scale sewage treatment. These problems limit the widespread application of microbial technology in actual sewage treatment. Summary of the invention

[0004] In order to solve the above problems, the present invention provides a method for preparing a microbial promoter for treating total nitrogen in sewage.

[0005] The preparation method of the microbial promoter is as follows:

[0006] S1. Add domesticated aerobic microorganism seed solution and domesticated nitrifying bacteria seed solution to beef extract-peptone liquid medium and nitrogen-free medium Ashu Bayer medium respectively, keep the temperature at 37°C, expose to light, and culture until OD 600 is 0.2;

[0007] S2, absorbing domesticated aerobic microorganisms and domesticated nitrifying bacteria in a mass ratio of 1:4 to prepare a microbial mixed solution;

[0008] S3, centrifuge the microbial mixed solution at 300 rpm for 25 min, remove the supernatant, and retain the microbial precipitate below;

[0009] S4, mixing nano zero-valent iron and microbial precipitate in a weight ratio of 7:1, adding 1 times the weight of glycerol, and stirring to mix evenly;

[0010] The microbial promoter is obtained through the above steps.

[0011] According to the above technical requirements, the domestication method of aerobic microorganisms is:

[0012] S1. Take 1-1.5 parts of domestic sewage by weight and add them to 10-15 parts of sterile water for dilution. Take 0.1 parts and inoculate them into beef extract-peptone medium. Keep the temperature at 37°C, expose to light, and culture for 36 hours. Select the colony with the largest bacterial colony.

[0013] S2, purifying the colonies respectively to obtain pre-tamed aerobic microorganisms;

[0014] S3. Add the pre-acclimated aerobic microorganisms to the beef extract-peptone liquid culture medium, keep the temperature at 37°C, and culture in the light until the OD 600 When it is 0.1, it is an aerobic microbial seed;

[0015] S4, sterilize one portion of the obtained sewage by wet heat sterilization at 120°C for 25 minutes, cool it to room temperature, and obtain sterilized sewage;

[0016] S5, adding 0.01 parts by weight of aerobic microbial seeds to the sterilized sewage, maintaining a constant temperature of 37°C and culturing in the light for 3-5 days to obtain secondary aerobic microbial seeds;

[0017] S6. Add 0.01 parts of secondary aerobic microbial seeds into the sterilized sewage, keep the temperature at 37°C, and culture in the light for 3-5 days. Repeat 5 times to obtain tertiary aerobic microbial seeds.

[0018] S7, add 0.01 parts of tertiary aerobic microbial seeds into unsterilized domestic sewage, keep the temperature at 37°C, incubate in the light for 3-5 days, repeat 3 times;

[0019] S8, purifying the tertiary aerobic microorganisms in S7;

[0020] Through the above steps, domesticated aerobic microorganisms can be obtained;

[0021] Identification of the bacterial flora showed domesticated aerobic microorganisms: Bacillus megaterium and Nocardia asteroides;

[0022] The preferred domesticated aerobic microorganism is Nocardia asteroides.

[0023] According to the above technical requirements, the domesticated aerobic microorganism seed solution includes: domesticated aerobic microorganisms and beef extract-peptone liquid culture medium, wherein the OD of the domesticated aerobic microorganism seed solution is 600 is 0.1.

[0024] According to the above technical requirements, the domestication method of nitrifying bacteria is:

[0025] S1. Take 1-1.5 parts of domestic sewage by weight and add them to 10-15 parts of sterile water for dilution. Take 0.1 parts and inoculate nitrogen-free culture medium Ashby's medium. Keep the temperature at 37°C, expose to light, and culture for 36 hours. Select the bacterial flora in the culture medium respectively.

[0026] S2, add the bacterial colony selected in S1 to the nitrogen-free medium Ashby's medium for cultivation, keep the temperature at 37°C, expose to light, cultivate for 36 hours, select the colonies, and repeat the cultivation 3-4 times;

[0027] S3, add the colonies in S2 to the nitrogen-free medium Ashby's medium, keep the temperature at 37°C, expose to light, and culture OD 600 to 0.1, which is the nitrifying bacteria seed solution;

[0028] S4, adding 0.01 parts by weight of nitrifying bacteria seed solution to the sterilized sewage, maintaining a constant temperature of 37° C., and culturing in the light for 3-5 days to obtain a secondary nitrifying bacteria seed solution;

[0029] S5. Add 0.01 parts of secondary nitrifying bacteria seed solution into the sterilized sewage, keep the temperature at 37°C, and culture in the light for 3-5 days. Repeat 5 times to obtain a tertiary nitrifying bacteria seed solution.

[0030] S6, add 0.01 parts of the tertiary nitrifying bacteria seed solution into the unsterilized domestic sewage, keep the temperature at 37°C, incubate in the light for 3-5 days, repeat 3 times;

[0031] S7, purifying the tertiary nitrifying bacteria seed solution in S6;

[0032] Through the above steps, domesticated nitrifying bacteria can be obtained;

[0033] Bacterial flora identification showed that the domesticated digestive bacteria were: Nitrobacter agilis and Nitrospira defluvii;

[0034] The preferred domesticated digestive bacterium is Nitrohelicobacter agilis.

[0035] According to the above technical requirements, nitrifying bacteria and nitrogen-free medium Ashu Bayer medium are domesticated, and the OD of the domesticated nitrifying bacteria seed solution is 600 is 0.1.

[0036] According to the above technical requirements, the formula of beef extract-peptone medium is:

[0037]

[0038] Agar: 15-20 g / L (if solid culture medium is required).

[0039] According to the above technical requirements, the formula of the nitrogen-free culture medium Ashu Bayer medium is:

[0040]

[0041]

[0042] According to the above technical requirements, the preparation method of nano zero-valent iron is:

[0043] Take 100mL of 0.04mg / L FeSO4 solution, add 0.1% to 5% PVPK-30, stir and mix evenly, then quickly add 100mL of 0.12mol / L NaBH4 solution, continue stirring for a few seconds, and stop stirring when the solution turns black. Select by magnetic separation, wash thoroughly with distilled water 3 times, wash with acetone 3 times, and store in acetone.

[0044] The preferred dosage of PVPK-30 is 3%;

[0045] According to the above technical requirements, PVPK-30 is: polyvinyl pyrrolidone.

[0046] According to the above technical requirements, microbial promoters can be used to treat total nitrogen in kitchen wastewater, bathroom wastewater, laundry wastewater, toilet wastewater, food processing wastewater, livestock and poultry breeding wastewater, and aquaculture wastewater.

[0047] Compared with the prior art, the present invention has the following beneficial effects:

[0048] 1) By using a combination of specifically domesticated microorganisms and nano-zero-valent iron, the removal efficiency of total nitrogen in sewage can be significantly improved. Compared with traditional biological and physical and chemical methods, this method can achieve the desired treatment effect faster;

[0049] 2) The preparation method of the microbial promoter of the present invention is simple and easy, the cost of the raw materials required is low, and the amount of chemical agents used can be reduced during use, thereby effectively reducing the cost of sewage treatment;

[0050] 3) The present invention utilizes microorganisms to naturally degrade total nitrogen in sewage, thus avoiding secondary pollution that may be caused by traditional chemical treatment methods, and is a more environmentally friendly treatment method;

[0051] 4) Specially domesticated microorganisms have strong adaptability to environmental changes. Even when conditions such as temperature, pH value or dissolved oxygen change, they can maintain high activity and ensure the stability of the treatment effect;

[0052] 5) This microbial promoter can not only be used to treat total nitrogen in domestic sewage, but can also be widely used in the treatment of total nitrogen in various types of sewage such as kitchen wastewater, bathroom wastewater, laundry wastewater, toilet wastewater, food processing wastewater, livestock and poultry breeding wastewater, and aquaculture wastewater. It has wide applicability and promotion value. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 The method is to prepare the microbial promoter for treating total nitrogen in sewage of the present invention by measuring the nitrogen content in domestic sewage before and after treatment by a Kjeldahl nitrogen analyzer; DETAILED DESCRIPTION

[0054] In order to further illustrate the method adopted by the present invention and the effect achieved, the technical solution of the present invention will be clearly and completely described in combination with experiments below.

[0055] Embodiment 1:

[0056] 1. Domestication of aerobic microorganisms:

[0057] S1. Take 10g of domestic sewage by weight and add it to 100g of sterile water for dilution. Take 1.1g of the solution and inoculate it into 100mL of beef extract-peptone medium. Keep the temperature at 37°C and expose it to light for 36h. Select the colony with the largest bacterial flora.

[0058] S2, purifying the colonies respectively to obtain pre-tamed aerobic microorganisms;

[0059] S3. Domesticating the pre-domesticated aerobic microorganisms:

[0060] S3-1. Add the pre-acclimated aerobic microorganisms to beef extract-peptone liquid culture medium, keep the temperature at 37°C, and culture in the light until the OD 600 When it is 0.1, it is an aerobic microbial seed;

[0061] S3-2, sterilize one portion of the obtained sewage by wet heat sterilization at 120°C for 25 min, and cool to room temperature to obtain sterilized sewage;

[0062] S3-3, add 1g of aerobic microbial seeds to 100mL of sterilized sewage, keep the temperature at 37°C, and culture in the light for 5 days to obtain secondary aerobic microbial seeds;

[0063] S3-4, add 1g of secondary aerobic microbial seeds into the sterilized sewage, keep the temperature at 37°C, incubate in the light for 5 days, repeat 5 times, and obtain tertiary aerobic microbial seeds;

[0064] S3-5, 1 g of tertiary aerobic microbial seeds was added to unsterilized domestic sewage, kept at 37°C, cultured in the light for 5 days, and repeated 3 times;

[0065] S3-6, purifying the tertiary aerobic microorganisms in S3-5;

[0066] Through the above steps, domesticated aerobic microorganisms can be obtained;

[0067] 2. Domesticate nitrifying bacteria:

[0068] S1. Take 10g of domestic sewage by weight and add it to 100g of sterile water for dilution. Take 1.1g of it and inoculate it into 100mL of nitrogen-free culture medium Ashby's medium. Keep the temperature at 37°C, expose to light, and culture for 36h. Select the bacterial flora in the culture medium respectively.

[0069] S2, add the bacterial colony selected in S1 to 100 mL of nitrogen-free medium Ashby's medium for cultivation, keep the temperature at 37°C, expose to light, cultivate for 36 hours, select colonies, and repeat the cultivation 4 times;

[0070] S3, add the colonies in S2 to 100 mL of nitrogen-free Aspergillus medium, keep the temperature at 37°C, expose to light, and culture OD 600 to 0.1, which is the nitrifying bacteria seed solution;

[0071] S4, add 1 g of nitrifying bacteria seed solution to 100 mL of sterilized sewage, keep the temperature at 37°C, and culture in the light for 5 days to obtain a secondary nitrifying bacteria seed solution;

[0072] S5. Add 1 g of secondary nitrifying bacteria seed solution into the sterilized sewage, keep the temperature at 37°C, and culture in the light for 5 days. Repeat 5 times to obtain a tertiary nitrifying bacteria seed solution.

[0073] S6, 1g of tertiary nitrifying bacteria seed solution was added to unsterilized domestic sewage, kept at 37°C, cultured in the light for 5 days, and repeated 3 times;

[0074] S7, purifying the tertiary nitrifying bacteria seed solution in S6;

[0075] Through the above steps, domesticated nitrifying bacteria can be obtained;

[0076] 3. Preparation of Nano Zero-Valent Iron:

[0077] Take 100mL of 0.04mg / L FeSO4 solution, add 3% PVPK-30, stir and mix well, then quickly add 100mL of 0.12mol / L NaBH4 solution, continue stirring for a few seconds, and stop stirring when the solution turns black. Select by magnetic separation, wash thoroughly with distilled water 3 times, wash with acetone 3 times, store in acetone, and spin dry the acetone before use;

[0078] 4. Preparation of microbial promoters:

[0079] 100 mL of beef extract-peptone liquid medium and 400 mL of nitrogen-free medium Ashur-Bai medium were added with domesticated aerobic microorganism seed solution and domesticated nitrifying bacteria seed solution, respectively, and cultured at a constant temperature of 37°C in the presence of light until OD 600 The mass ratio of domesticated aerobic microorganisms to domesticated nitrifying bacteria is 1:4 to prepare a microbial mixed solution. The microbial mixed solution is centrifuged at 300 rpm for 25 min, the supernatant is removed, 35 g of the microbial precipitate below is retained, 245 g of nano zero-valent iron is added, stirred and mixed, 280 g of glycerol is added, and the mixture is stirred and mixed evenly to obtain a microbial promoter.

[0080] Treatment Group 1:

[0081] The total nitrogen in sewage was analyzed using the microbial promoter prepared in Example 1:

[0082] Filter 1L of domestic sewage, retain the aqueous phase, add 5g of microbial promoter, stir, keep a constant temperature of 37°C, expose to light, culture for 5 days, centrifuge at 1000 rpm for 25 minutes, retain the upper aqueous phase, centrifuge the aqueous phase at 3000 rpm for 25 minutes to ensure that all microorganisms and nano-zero-valent iron are precipitated, and measure the nitrogen content in the domestic sewage before and after treatment using a Kjeldahl nitrogen analyzer.

[0083] Comparative treatment group 1:

[0084] Filter 1L of domestic sewage, retain the aqueous phase, add 5g of domesticated aerobic microbial seed solution, stir, keep a constant temperature of 37°C, expose to light, culture for 5 days, centrifuge at 1000 rpm for 25 minutes, retain the upper aqueous phase, centrifuge the aqueous phase at 3000 rpm for 25 minutes to ensure that all microorganisms are precipitated, and measure the nitrogen content in the domestic sewage before and after treatment using a Kjeldahl nitrogen analyzer.

[0085] Comparative treatment group 2:

[0086] Filter 1L of domestic sewage, retain the aqueous phase, add 5g of domesticated nitrifying bacteria seed solution, stir, keep a constant temperature of 37℃, expose to light, culture for 5 days, centrifuge at 1000 rpm for 25 minutes, retain the upper aqueous phase, centrifuge the aqueous phase at 3000 rpm for 25 minutes to ensure that all microorganisms are precipitated, and measure the nitrogen content in the domestic sewage before and after treatment using a Kjeldahl nitrogen analyzer.

[0087] Comparative treatment group 3:

[0088] Filter 1L of domestic sewage, retain the aqueous phase, add 2.5g of domesticated nitrifying bacteria seed solution and 2.5g of domesticated aerobic microorganism seed solution, stir, keep a constant temperature of 37°C, expose to light, culture for 5 days, centrifuge at 1000 rpm for 25 minutes, retain the upper aqueous phase, centrifuge the aqueous phase at 3000 rpm for 25 minutes to ensure that all microorganisms are precipitated, and measure the nitrogen content in the domestic sewage before and after treatment using a Kjeldahl nitrogen analyzer.

[0089] Table 1 shows the nitrogen content in domestic sewage before and after treatment measured by Kjeldahl nitrogen analyzer.

[0090] Table 1

[0091] Serial number Before treatment After treatment Processing rate Treatment Group 1 74.3mg / L 6.2mg / L 92％ Comparative treatment group 1 74.3mg / L 50.6mg / L 32％ Comparative treatment group 2 74.3mg / L 32.8mg / L 56％ Comparative treatment group 3 74.3mg / L 21.7mg / L 71％

[0092] It can be concluded from the data in Table 1 that treatment group 1 used the microbial promoter prepared by the present invention, and the total nitrogen content in the treated sewage was reduced from 74.3 mg / L to 6.2 mg / L, and the treatment rate reached 92%, indicating that the method can significantly improve the removal efficiency of total nitrogen in sewage. Comparative treatment group 1 was treated only with domesticated aerobic microbial seed solution, and the total nitrogen content after treatment was 50.6 mg / L, and the treatment rate was 32%; comparative treatment group 2 was treated only with domesticated nitrifying bacteria seed solution, and the total nitrogen content after treatment was 32.8 mg / L, and the treatment rate was 56%. This shows that the treatment effect of a single type of microorganism is limited and cannot meet the expected treatment standard. Comparative treatment group 3 used both domesticated nitrifying bacteria seed solution and domesticated aerobic microbial seed solution, and the total nitrogen content after treatment was 21.7 mg / L, and the treatment rate was 71%. Although the effect is better than the use of any single microorganism, it is still lower than the effect of treatment group 1. The main difference between treatment group 1 and comparative treatment group 3 is whether nano zero-valent iron is added. Nano-zero-valent iron was added to treatment group 1, and the treatment effect was significantly better than that of control treatment group 3, which shows that nano-zero-valent iron plays an important role in improving the efficiency of microbial treatment of total nitrogen in sewage. In summary, the present invention can significantly improve the removal efficiency of total nitrogen in sewage through the combination of specific domesticated microorganisms and nano-zero-valent iron, and has higher treatment efficiency and better environmental adaptability than traditional methods.

Claims

1. A method for preparing a microbial promoter for treating total nitrogen in sewage, characterized in that: The following steps are involved: S1. Add domesticated aerobic microorganism seed solution and domesticated nitrifying bacteria seed solution to beef extract-peptone liquid medium, nitrogen-free medium and Ashu-Bei's medium respectively, keep the temperature at 37°C, expose to light, and culture until OD 600 is 0.2; S2, absorbing domesticated aerobic microorganisms and domesticated nitrifying bacteria in a mass ratio of 1:4 to prepare a microbial mixed solution; S3, centrifuge the microbial mixed solution at 300 rpm for 25 min, remove the supernatant, and retain the microbial precipitate below; S4, mixing nano zero-valent iron and microbial precipitate in a weight ratio of 7:1, adding 1 times the weight of glycerol, and stirring to mix evenly; The microbial promoter is obtained through the above steps.

2. The method for preparing a microbial promoter for treating total nitrogen in sewage according to claim 1, characterized in that: The domestication method of aerobic microorganisms is: S1. Take 1-1.5 parts of domestic sewage by weight and add them to 10-15 parts of sterile water for dilution. Take 0.1 parts and inoculate them into beef extract-peptone medium. Keep the temperature at 37°C, expose to light, and culture for 36 hours. Select the colony with the largest bacterial colony. S2, purifying the colonies respectively to obtain pre-tamed aerobic microorganisms; S3. Add the pre-acclimated aerobic microorganisms to the beef extract-peptone liquid culture medium, keep the temperature at 37°C, and culture in the light until the OD 600 When it is 0.1, it is an aerobic microbial seed; S4, sterilize one portion of the obtained sewage by wet heat sterilization at 120°C for 25 minutes, cool it to room temperature, and obtain sterilized sewage; S5, adding 0.01 parts by weight of aerobic microbial seeds to the sterilized sewage, maintaining a constant temperature of 37°C and culturing in the light for 3-5 days to obtain secondary aerobic microbial seeds; S6. Add 0.01 parts of secondary aerobic microbial seeds into the sterilized sewage, keep the temperature at 37°C, and culture in the light for 3-5 days. Repeat 5 times to obtain tertiary aerobic microbial seeds. S7, add 0.01 parts of tertiary aerobic microbial seeds into unsterilized domestic sewage, keep the temperature at 37°C, incubate in the light for 3-5 days, repeat 3 times; S8, purifying the tertiary aerobic microorganisms in S7; The domesticated aerobic microorganisms can be obtained through the above steps.

3. The method for preparing a microbial promoter for treating total nitrogen in sewage according to claim 1, characterized in that: The acclimated aerobic microorganism seed solution comprises: acclimated aerobic microorganisms and beef extract-peptone liquid culture medium, wherein the OD of the acclimated aerobic microorganism seed solution is 600 is 0.

1.

4. The method for preparing a microbial promoter for treating total nitrogen in sewage according to claim 1, characterized in that: The domestication method of nitrifying bacteria is: S1. Take 1-1.5 parts of domestic sewage by weight and add them to 10-15 parts of sterile water for dilution. Take 0.1 parts and inoculate nitrogen-free culture medium Ashby's medium. Keep the temperature at 37°C, expose to light, and culture for 36 hours. Select the bacterial flora in the culture medium respectively. S2, add the bacterial colony selected in S1 to the nitrogen-free medium Ashby's medium for cultivation, keep the temperature at 37°C, expose to light, cultivate for 36 hours, select the colonies, and repeat the cultivation 3-4 times; S3, add the colonies in S2 to the nitrogen-free medium Ashby's medium, keep the temperature at 37°C, expose to light, and culture OD 600 to 0.1, which is the nitrifying bacteria seed solution; S4, adding 0.01 parts by weight of nitrifying bacteria seed solution to the sterilized sewage, maintaining a constant temperature of 37° C., and culturing in the light for 3-5 days to obtain a secondary nitrifying bacteria seed solution; S5. Add 0.01 parts of secondary nitrifying bacteria seed solution into the sterilized sewage, keep the temperature at 37°C, and culture in the light for 3-5 days. Repeat 5 times to obtain a tertiary nitrifying bacteria seed solution. S6, add 0.01 parts of the tertiary nitrifying bacteria seed solution into the unsterilized domestic sewage, keep the temperature at 37°C, incubate in the light for 3-5 days, repeat 3 times; S7, purifying the tertiary nitrifying bacteria seed solution in S6; The domesticated nitrifying bacteria can be obtained through the above steps.

5. The method for preparing a microbial promoter for treating total nitrogen in sewage according to claim 1, characterized in that: The domesticated nitrifying bacteria seed solution includes: domesticated nitrifying bacteria and nitrogen-free culture medium Ashu Bayer medium, wherein the OD of the domesticated nitrifying bacteria seed solution is 600 is 0.

1.

6. The method for preparing a microbial promoter for treating total nitrogen in sewage according to any one of claims 1 to 2, characterized in that: The formula of beef extract-peptone medium is: Beef extract 3g / L; Peptone 10 g / L; Sodium chloride 5g / L; Water 1000mL; Agar 15-20g / L.

7. A method for preparing a microbial promoter for treating total nitrogen in sewage according to any one of claims 1 to 4, characterized in that: The formula of nitrogen-free medium Ashby's medium is:

8. The method for preparing a microbial promoter for treating total nitrogen in sewage according to claim 1, characterized in that: The preparation method of nano zero-valent iron is: Take 100mL of 0.04mg / L FeSO4 solution, add 0.1% to 5% PVPK-30, stir and mix evenly, then quickly add 100mL of 0.12mol / L NaBH4 solution, continue stirring for a few seconds, stop stirring when the solution turns black, select it by magnetic separation, wash it thoroughly with distilled water 3 times, wash it with acetone 3 times, and store it in acetone.

9. The method for preparing a microbial promoter for treating total nitrogen in sewage according to claim 8, characterized in that: PVPK-30 is: polyvinyl pyrrolidone.

10. The method for preparing a microbial promoter for treating total nitrogen in sewage according to claim 1, characterized in that: Microbial promoters are used to treat total nitrogen in kitchen wastewater, bathroom wastewater, laundry wastewater, toilet wastewater, food processing wastewater, livestock and poultry breeding wastewater, and aquaculture wastewater.

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