Device and method for improving biological detection efficiency and flux of two-dimensional sensing carrier

Abstract

The invention belongs to the technical field of biological experiment equipment, and particularly relates to a device and method for improving the biological detection efficiency and flux of a two-dimensional sensing carrier. The device comprises a reaction chamber integrated board, a multi-connected electromagnetic clamp, a three-dimensional driving module, a groove module, a heating and temperature control module, an air blowing module and a man-machine interaction module. The reaction chamber integrated board consists of a plurality of small chambers with the same specification and is used as a reaction container for biological detection; the multi-connected electromagnetic clamp is used for clamping a two-dimensional sensing chip; and the groove module is used for placing the reaction chamber integrated board and the cleaning pool so as to realize reaction and cleaning of the two-dimensional sensing chip. The device is suitable for biosensing scientific research work based on a two-dimensional sensing chip, can remarkably improve the operation efficiency, repeatability and accuracy of researchers on a biological detection experiment, and is beneficial to promoting the automation and high-throughput improvement of biosensing detection.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological experimental equipment, and specifically relates to a device and method for improving the biological detection efficiency and flux of a two-dimensional sensing carrier. Background Art

[0002] Biosensors are another important sensing field besides physical sensors and chemical sensors. They achieve qualitative and quantitative detection of targets by converting the changes in physical and chemical quantities produced by the specific interaction between sensitive elements and targets into electrical signals. Biosensors usually have the characteristics of high sensitivity, good selectivity, and strong controllability. They play an indispensable role in medical testing, environmental monitoring, and epidemic prevention and control, and are also an important development direction in the field of scientific research.

[0003] In the process of using biosensors and the research of related detection technologies, separation operation is an essential step. ELISA (enzyme-linked immunosorbent assay) technology is the gold standard for biological detection. In this technology, the separation of the ELISA plate and the reaction solution is usually carried out by pouring and drying. After that, the ELISA plate can be held in reverse and hit against absorbent paper to achieve a full separation effect. However, although this method has excellent separation effect, it is usually only applicable to ELISA technology that has been standardized. In addition, the magnetic separation mode is a common universal detection mode in biological detection. This mode uses magnetic microspheres (magnetic beads) as carriers and performs non-contact separation through magnetic force. It is easy to operate, fast and efficient. However, for electrochemical, photoelectric, piezoelectric and other sensors, the magnetic separation mode is often not applicable because the magnetic microspheres will have a significant impact on the test results. For these sensors, the carriers relied on for detection are two-dimensional planar sensor chips such as screen-printed electrodes, interdigitated electrodes, conductive glass, and piezoelectric chips. The separation mode adopted is similar to the ELISA method, but because no standardized measures are taken, the research process often causes great trouble, making the whole process time-consuming and prone to operational errors and errors. At the same time, these planar carriers can only process one at a time during separation and cleaning, which makes it difficult to process in parallel with high throughput, greatly reducing the sensing efficiency, and further reducing the repeatability and accuracy.

[0004] Taking screen-printed electrodes as an example, their application in biosensor detection usually includes nanomaterial modification, antibody (or aptamer, molecular imprinting polymer and other functional materials that can be used to specifically capture the target object to be detected) functional modification, reaction with the test liquid, washing, reaction with the labeled probe, washing, etc., and finally signal detection. Each of the above steps involves the reaction process and separation process, and its consistency is difficult to guarantee. Once multiple groups of samples are processed simultaneously, researchers are often in a hurry, and efficiency and accuracy will be greatly affected.

[0005] Therefore, in order to fill the gap in existing technologies, it is urgent to develop high-throughput and high-efficiency bioassay auxiliary devices for two-dimensional sensing carriers. Summary of the invention

[0006] The purpose of the present invention is to disclose a device and method for improving the efficiency and flux of biological detection of two-dimensional sensing carriers in view of the deficiencies of the prior art. The device forms a multi-connected parallel structure (i.e., a multi-connected electromagnetic fixture and a reaction chamber integrated board) by connecting the card slots and reaction chambers with matching layouts and sizes in parallel, which converts the manual single processing of traditional two-dimensional chips into simultaneous processing of multiple chips, greatly improving the detection flux. At the same time, the device can overcome the problems of long manual operation, multiple sources of errors, and non-standardization during the detection process, and significantly improve the detection efficiency, repeatability, and accuracy. The invention has great practical value in the fields of biological sensor detection based on two-dimensional chips, and is particularly suitable for the research and exploration work of scientific researchers in related fields.

[0007] To achieve the above technical objectives, the technical solution of the present invention is as follows:

[0008] In a first aspect, the present invention provides a method for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier, using a device, the device comprising a reaction chamber integrated board 1, a multi-unit electromagnetic fixture 3, a three-dimensional driving module, a groove module, a heating and temperature control module 8, a blowing module, and a human-computer interaction module 9;

[0009] The reaction chamber integrated board 1 is composed of small chambers 2 of the same specifications arranged in parallel and placed at the bottom of the device;

[0010] The multi-unit electromagnetic clamp 3 is used to clamp the two-dimensional sensor chip 20. The number of clamp units on the multi-unit electromagnetic clamp 3 is not less than the number of small chambers 2 in the reaction chamber integrated board 1. Each clamp unit in the multi-unit electromagnetic clamp 3 is composed of a clamp control module 17 connecting two symmetrical first clamps 18 and second clamps 19. The clamp control module 17 has a built-in clamping device that changes the magnetic field strength by controlling the input current, thereby adjusting the strength of the clamp to clamp the two-dimensional sensor chip 20, so as to adapt to chips of different thicknesses and materials.

[0011] The multi-unit electromagnetic fixture 3 is loaded on a three-dimensional driving module, which is a three-axis servo motor driving module or a manipulator, and is used to move the multi-unit electromagnetic fixture 3 in the device; by adjusting the three-dimensional driving module, the two-dimensional sensor chip 20 in the multi-unit electromagnetic fixture 3 corresponds to the small chamber 2 in the reaction chamber integrated board 1 one by one and is in a suitable position, so as to ensure the smooth progress of the biological recognition and binding process;

[0012] The groove module includes a reaction chamber groove and a cleaning pool groove 5, which are located at one side of the bottom of the device and are used to place the reaction chamber integrated board 1 and the cleaning pool respectively; wherein the reaction chamber groove matches the size of the reaction chamber integrated board 1, and the cleaning pool groove 5 matches the size of the cleaning pool; a cleaning liquid is placed in the cleaning pool;

[0013] The heating and temperature control module 8 is located below the groove module and is used to heat the reaction liquid and the cleaning liquid in the reaction chamber integrated plate 1 and the cleaning pool to meet the temperature requirements of the reaction process with special temperature requirements; for example, the immune binding process usually requires the temperature to be set to 37°C, and the formation or decoupling of nucleic acid double strands requires a higher temperature;

[0014] The blowing module is located at the other side of the bottom of the device, opposite to the groove module, and the two modules are separated by a partition 7 to prevent the blown liquid from splashing into the reaction liquid or the cleaning liquid to cause pollution; the blowing module includes a speed-adjustable fan 16, which blows air in the parallel direction of the two-dimensional sensor chip 20 array to remove residual liquid on the chip surface to prevent interference with the next reaction or cleaning process;

[0015] The human-machine interaction module 9 is provided with control instructions and is communicatively connected to the multi-unit electromagnetic fixture 3, the three-dimensional driving module, the heating and temperature control module 8, and the blowing module for observing and manipulating the instrument;

[0016] The specific steps are as follows:

[0017] The first step is to insert a plurality of two-dimensional planar electrodes 20 into the corresponding multi-unit electromagnetic fixtures 3, and perform corresponding functional modification on the electrodes according to the detection requirements;

[0018] In the second step, solutions containing target objects of different concentrations, including test liquid and detection liquid, are added to the small chamber 2 of the reaction chamber integrated board 1, and placed in the reaction chamber groove; cleaning liquid is added to the cleaning pool and placed in the cleaning pool groove 5.

[0019] The third step is to control parameters through the human-computer interaction module 9, including the number of cleaning times, cleaning soaking time, reaction time of each reaction chamber, reaction temperature, and blowing time;

[0020] The operation process is started. Under the control of the three-dimensional driving module, the functionally modified two-dimensional planar electrode 20 array is positioned to the corresponding position of the reaction chamber groove; the two-dimensional planar electrode 20 is immersed in the solution and reacts in each reaction chamber in sequence according to the setting;

[0021] During this period, if the reaction chamber needs to be cleaned before entering a new reaction chamber, the electrode array is cleaned in the cleaning tank and positioned under the speed-adjustable fan 16 to blow out the residual liquid;

[0022] The fourth step is to insert the processed planar electrode into the sensor, add the detection liquid, perform signal detection, record the data, and complete the detection process.

[0023] Preferably, when performing electrochemiluminescence biosensor detection, two reaction chamber grooves are provided, namely, reaction chamber groove 1 4 and reaction chamber groove 2 6, and the following steps are specifically included:

[0024] Add the test liquids containing different concentrations of the target object into the small chamber 2 of the No. 1 reaction chamber integrated board 1 one by one, and place them in the groove 4 of the No. 1 reaction chamber; add the electrochemical luminescent material labeled secondary antibody solution into the No. 2 reaction chamber integrated board 1, and place it in the groove 6 of the No. 2 reaction chamber; add the PBST cleaning solution into the cleaning pool, which is divided into small chambers matching the size of the multi-unit electromagnetic fixture 3, and place them in the groove 5 of the cleaning pool;

[0025] The number of cleaning times, the soaking time for each cleaning, the reaction time of reaction chamber No. 1, the reaction time of reaction chamber No. 2, and the reaction temperature blowing time are controlled by the human-computer interaction module 9 to start the operation process;

[0026] The two-dimensional planar electrode 20 chip array is positioned at the corresponding position of the groove 4 of the reaction chamber No. 1, completely immersed in the test solution, reacted in a 37°C environment, and the target is captured on the electrode surface; thereafter, the electrode array is positioned under the speed-adjustable fan 16 to blow out the residual liquid; after cleaning, the electrode array is positioned at the groove 6 of the reaction chamber No. 2, reacted in a 37°C environment, and the electrochemiluminescent labeled secondary antibody is combined with the target to construct a double antibody sandwich structure;

[0027] After cleaning and drying, the treated planar electrode is inserted into the electrochemiluminescence sensor, and the detection liquid is added, and the electrochemiluminescence signal is detected, and the data is recorded to complete the detection process.

[0028] Furthermore, the two-dimensional planar electrode 20 for electrochemiluminescent biosensor detection is subjected to the following functional modification treatments:

[0029] A dopamine potassium carbonate buffer solution was prepared and the pH value was adjusted to 9.6, and a saturated concentration of nanomaterials was added thereto, and the nanomaterials were evenly dispersed and added to the No. 1 reaction chamber integrated board;

[0030] Add the target-specific antibody solution to the No. 2 reaction chamber integrated board and place it in the No. 2 reaction chamber groove 4; add the PBST cleaning solution to the cleaning pool, which is divided into small chambers matching the size of the multi-unit electromagnetic fixture 3 and placed in the cleaning pool groove 5.

[0031] The electrode array is assembled, and the parameters are adjusted through the human-computer interaction module 9 to start the operation process; the electrode chip array is carried in the multi-unit electromagnetic fixture 3 to the corresponding position of the groove 4 of the No. 1 reaction chamber, and is completely immersed in the mixed solution of nanomaterials and dopamine to react, and the nanomaterials are fixed on the electrode surface through the formation of polydopamine; the electrode array is driven to the speed-adjustable fan 16 to quickly blow out the residual liquid; after blowing away the residual liquid, the cleaning operation is performed according to the set cleaning and soaking, and the residual liquid must be blown away after each soaking;

[0032] After cleaning, the electrode array is driven to the groove 6 of the No. 2 reaction chamber and soaked for a set time to combine the antibody with polydopamine through a covalent reaction to achieve functional modification of the antibody on the electrode surface.

[0033] In a second aspect, the present invention provides a device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier used in the method described in the first aspect.

[0034] Preferably, the device is provided with a housing, and a human-machine interaction module 9 is located on the side of the housing of the device, for an operator to observe parameters and operate the device.

[0035] Preferably, the solution capacity of the chamber 2 of the reaction chamber integrated plate 1 is 50 to 500 microliters, and the integrated number is 5 to 20.

[0036] Preferably, the three-dimensional driving module includes an X-axis servo motor 10, a Y-axis servo motor guide 11, a Z-axis servo motor 12, a Z-axis servo motor guide 13, an X-axis servo motor guide 14, and a Y-axis servo motor 15; the multi-unit electromagnetic clamp 3 is fixed to the end of the Z-axis servo motor guide 13, the Z-axis servo motor 12 and the Z-axis servo motor guide 13 are located in the Y-axis servo motor guide 11, and the X-axis servo motor guide 14 is arranged vertically to the Y-axis servo motor guide 11; under the drive of multiple servo motors, the multi-unit electromagnetic clamp 3 and the two-dimensional sensor chip 20 therein can move arbitrarily inside the device.

[0037] The three-dimensional driving module is located at the top of the entire device and is used to control the movement of the multi-unit electromagnetic fixture 3 in three-dimensional space, thereby meeting the movement and position requirements of the sensor chip array in the steps of reaction, cleaning, etc. The three-dimensional driving module is the basis for the automation of this device.

[0038] Preferably, the temperature control range of the heating and temperature control module 8 is 20-100°C.

[0039] Preferably, the number of grooves in the groove module is 3 to 8.

[0040] Preferably, the reaction chamber integrated plate 1 is formed by composite nesting or integral molding of independent small chambers 2 of the same specifications.

[0041] Beneficial effects achieved by the present invention:

[0042] (1) High detection throughput, multiple two-dimensional sensor chips can be processed in parallel, which substantially improves efficiency compared to the single-chip processing method in traditional scientific research;

[0043] (2) Strong repeatability and high accuracy. Since the operation process and treatment conditions of each sensor chip are basically the same during parallel processing, the error caused by human factors is greatly reduced, which can significantly improve the repeatability of the detection process and the accuracy of the detection results;

[0044] (3) Wide range of applications: the device can match two-dimensional sensor chips of different thicknesses, lengths, and widths, bringing convenience to biosensor research and exploration;

[0045] (4) The device can realize the automation of the detection process, which not only speeds up the chip processing speed and accuracy, but also provides ideas and foundations for future automation and intelligent improvements. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 It is a schematic diagram of the overall structure of the device of the present invention;

[0047] Figure 2 It is a schematic diagram of the operation of the two-dimensional sensor chip applied to the present invention. DETAILED DESCRIPTION

[0048] To facilitate understanding of the present invention, a comprehensive and detailed explanation will be given below in conjunction with the embodiments and drawings of the specification, but the protection scope of the present invention is not limited to the following specific embodiments.

[0049] Example 1

[0050] A device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier, the device comprising a reaction chamber integrated board 1, a multi-unit electromagnetic fixture 3, a three-dimensional driving module, a groove module, a heating and temperature control module 8, an air blowing module, and a human-computer interaction module 9;

[0051] The reaction chamber integrated board 1 is composed of small chambers 2 of the same specifications arranged in parallel and placed at the bottom of the device;

[0052] The multi-unit electromagnetic clamp 3 is used to clamp the two-dimensional sensor chip 20. The number of clamp units on the multi-unit electromagnetic clamp 3 is the same as the number of chambers 2 in the reaction chamber integrated board 1. Each clamp unit in the multi-unit electromagnetic clamp 3 is composed of a clamp control module 17 connecting two symmetrical first clamps 18 and second clamps 19. The clamp control module 17 has a built-in clamping device that changes the magnetic field strength by controlling the input current, thereby regulating the strength of the clamp clamping the two-dimensional sensor chip 20.

[0053] The multi-mounted electromagnetic fixture 3 is loaded on a three-dimensional driving module, which is a three-axis servo motor driving module, and is used to displace the multi-mounted electromagnetic fixture 3 in the device; by adjusting the three-dimensional driving module, the two-dimensional sensor chip 20 in the multi-mounted electromagnetic fixture 3 corresponds one-to-one with the small chamber 2 in the reaction chamber integrated board 1 and is in a suitable position, so as to ensure the smooth progress of the biological recognition and binding process; the three-dimensional driving module includes an X-axis servo motor 10, a Y-axis servo motor guide 11, a Z-axis servo motor 12, a Z-axis servo motor guide 13, an X-axis servo motor guide 14, and a Y-axis servo motor 15; the multi-mounted electromagnetic fixture 3 is fixed to the end of the Z-axis servo motor guide 13, the Z-axis servo motor 12 and the Z-axis servo motor guide 13 are located in the Y-axis servo motor guide 11, and the X-axis servo motor guide 14 is arranged vertically with the Y-axis servo motor guide 11; under the drive of multiple servo motors, the multi-mounted electromagnetic fixture 3 and the two-dimensional sensor chip 20 therein can be moved arbitrarily inside the device.

[0054] The groove module includes a reaction chamber groove and a cleaning pool groove 5, which are located at one side of the bottom of the device and are used to place the reaction chamber integrated board 1 and the cleaning pool respectively; wherein the reaction chamber groove includes a No. 1 reaction chamber groove 4 and a No. 2 reaction chamber groove 6, which match the size of the reaction chamber integrated board 1, and the size of the cleaning pool groove 5 matches the cleaning pool; the cleaning liquid is placed in the cleaning pool;

[0055] The heating and temperature control module 8 is located below the groove module and is used to heat the reaction liquid and the cleaning liquid in the reaction chamber integrated plate 1 and the cleaning pool to meet the temperature requirements of the reaction process with special temperature requirements; for example, the immune binding process usually requires the temperature to be set to 37°C, and the formation or decoupling of nucleic acid double strands requires a higher temperature;

[0056] The blowing module is located at the other side of the bottom of the device, opposite to the groove module, and the two modules are separated by a partition 7 to prevent the blown liquid from splashing into the reaction liquid or the cleaning liquid to cause pollution; the blowing module includes a speed-adjustable fan 16, which blows air in the parallel direction of the two-dimensional sensor chip 20 array to remove residual liquid on the chip surface to prevent interference with the next reaction or cleaning process;

[0057] The human-machine interaction module 9 is provided with control instructions and is communicatively connected with the multi-unit electromagnetic fixture 3, the three-dimensional driving module, the heating and temperature control module 8, and the blowing module for observing and operating the instrument.

[0058] Example 2

[0059] This embodiment is an example process and method for applying the sensitivity enhancement modification and functionalization modification of the two-dimensional sensor chip in embodiment 1. The so-called sensitivity enhancement modification refers to the use of nanomaterials or other materials that can improve the detection sensitivity to modify the chip surface, and the functionalization modification refers to the modification of specific sensitive probe antibodies, aptamers, nucleic acid chains, molecular imprinting polymers, etc. on the electrode surface.

[0060] First, a dopamine potassium carbonate buffer is prepared and the pH value is adjusted to 9.6, and a saturated concentration of nanomaterial nanogold is added thereto, and the nanomaterial nanogold is evenly dispersed and added to the reaction chamber integrated board 1. Since the modification of all electrodes performs exactly the same operation, the reaction chamber integrated board 1 does not need a small chamber at this time. The reaction chamber integrated board is placed in the groove 4 of the reaction chamber No. 1.

[0061] The target specific antibody solution is added to another identical reaction chamber integrated plate 1 and placed in the groove 4 of reaction chamber No. 2. PBST cleaning solution 0.01 mol / L, containing 0.02% Tween-20, pH=7.4 is added to the cleaning pool, which is divided into 6 small chambers matching the size of the multi-unit electromagnetic fixture 3, and placed in the groove 5 of the cleaning pool.

[0062] The electrode array is assembled, and the parameters such as the number of cleaning times, the soaking time for each cleaning, the reaction time of the No. 1 reaction chamber, the reaction time of the No. 2 reaction chamber, and the blowing time are controlled by the human-computer interaction module 9 to start the operation process. After the device is started, under the control of the three-dimensional driving module, the electrode chip array is carried to the corresponding position of the groove 4 of the No. 1 reaction chamber in the multi-mounted electromagnetic clamp 3. It is completely immersed in the mixed solution of nanomaterials and dopamine, reacts for the set time, and fixes the nanomaterials on the electrode surface through the formation of polydopamine. Thereafter, the electrode array is driven to the speed-adjusting fan 16 to quickly blow out the residual liquid. The device can be observed from the outside. If the effect is not achieved, the wind speed or time can be continued to be increased through the operation interface; after blowing away the residual liquid, the cleaning times are set according to the set number of cleaning times. This time, the set number of times is 3 times, and the cleaning operation is performed corresponding to the 6 small chambers of the cleaning pool and the soaking time for each cleaning. The residual liquid must be blown away after each soaking. After cleaning, the electrode array is driven to groove 6 of reaction chamber No. 2 and immersed to combine the antibody with polydopamine through covalent reaction, thereby realizing functional modification of the antibody on the electrode surface. After three cleanings, the next step of formal detection operation can be performed.

[0063] Example 3

[0064] This embodiment is a process and method for applying the two-dimensional sensor chip processed in Example 2 to electrochemiluminescence biosensor detection. The process and method are basically applicable to other label-type biosensors.

[0065] In the first step, according to the method of Example 1, the two-dimensional planar electrodes 20 are inserted one by one into the multi-unit electromagnetic fixture 3. The two-dimensional planar electrodes 20 have been functionally modified by the method shown in Example 2.

[0066] In the second step, the test solution of the target object with gradient concentration is added to the chamber 2 of the No. 1 reaction chamber integrated board 1 one by one, and it is placed in the groove 4 of the No. 1 reaction chamber. The electrochemical luminescent material terpyridine ruthenium labeled secondary antibody solution is added to the No. 2 reaction chamber integrated board 1, and it is placed in the groove 6 of the No. 2 reaction chamber. PBST cleaning solution 0.01 mol / L, containing 0.02% Tween-20, pH=7.4 is added to the cleaning pool, and the cleaning pool is divided into 6 small chambers matching the size of the multi-unit electromagnetic fixture 3, which are placed in the cleaning pool groove 5.

[0067] In the third step, with reference to Example 2, the human-computer interaction module 9 is used to control the cleaning times 3 times, the soaking time for each cleaning is 5 minutes, the reaction time of the No. 1 reaction chamber is 30 minutes, the reaction time of the No. 2 reaction chamber is 30 minutes, the reaction temperature is 37°C, and the blowing time parameters are started. Under the control of the three-dimensional driving module, the functionalized modified electrode chip array is positioned to the corresponding position of the groove 4 of the No. 1 reaction chamber, completely immersed in the solution to be tested, reacted in a 37°C environment for 30 minutes, and the target is captured on the electrode surface. Thereafter, the electrode array is positioned under the speed-adjusting fan 16, the residual liquid is blown out quickly, and the cleaning is repeated 3 times. After the cleaning is completed, the electrode array is positioned at the groove 6 of the No. 2 reaction chamber, reacted in a 37°C environment for 30 minutes, and the electrochemiluminescent labeled secondary antibody is combined with the target to construct a double antibody sandwich structure. After three cleanings and drying, the planar electrode after the treatment is inserted into the electrochemiluminescent sensor, the detection liquid is dripped, the electrochemiluminescent signal is detected, the data is recorded, and the detection process is completed.

Claims

1. A method for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier, characterized in that: A device is used, the device comprising a reaction chamber integrated board (1), a multi-unit electromagnetic clamp (3), a three-dimensional driving module, a groove module, a heating and temperature control module (8), an air blowing module, and a human-computer interaction module (9); The reaction chamber integrated plate (1) is composed of small chambers (2) of the same specifications arranged in parallel and placed at the bottom of the device; The multi-unit electromagnetic clamp (3) is used to clamp the two-dimensional sensor chip (20), and the number of clamp units on the multi-unit electromagnetic clamp (3) is not less than the number of small chambers (2) in the reaction chamber integrated board (1); each clamp unit in the multi-unit electromagnetic clamp (3) is composed of a clamp control module (17) connecting two symmetrical first clamps (18) and second clamps (19), and the clamp control module (17) is built-in with a clamping device that changes the magnetic field strength by controlling the input current, thereby adjusting the strength of the clamp to clamp the two-dimensional sensor chip (20) to adapt to chips of different thicknesses and materials; The multi-unit electromagnetic fixture (3) is loaded on a three-dimensional driving module, and the three-dimensional driving module is a three-axis servo motor driving module or a manipulator, which is used to move the multi-unit electromagnetic fixture (3) in the device; by adjusting the three-dimensional driving module, the two-dimensional sensor chip (20) in the multi-unit electromagnetic fixture (3) and the small chamber (2) in the reaction chamber integrated board (1) are in one-to-one correspondence and are in a suitable position, thereby ensuring the smooth progress of the biological recognition and binding process; The groove module comprises a reaction chamber groove and a cleaning pool groove (5), which are located at one side of the bottom of the device and are used to place the reaction chamber integrated board (1) and the cleaning pool respectively; wherein the reaction chamber groove matches the size of the reaction chamber integrated board (1), and the cleaning pool groove (5) matches the size of the cleaning pool; cleaning liquid is placed in the cleaning pool; The heating and temperature control module (8) is located below the groove module and is used to heat the reaction liquid and the cleaning liquid in the reaction chamber integrated board (1) and the cleaning pool to meet the temperature requirements of the reaction process with special temperature requirements; for example, the immune binding process usually requires the temperature to be set at 37°C, and the formation or decoupling of nucleic acid double strands requires a higher temperature; The blowing module is located at the other side of the bottom of the device, opposite to the groove module, and the two modules are separated by a partition (7) to prevent the blown liquid from splashing into the reaction liquid or the cleaning liquid to cause pollution; the blowing module includes a speed-adjustable fan (16) that blows air in a direction parallel to the two-dimensional sensor chip (20) array to remove residual liquid on the chip surface to prevent interference with the next reaction or cleaning process; The human-machine interaction module (9) is provided with control instructions and is communicatively connected to the multi-unit electromagnetic clamp (3), the three-dimensional drive module, the heating and temperature control module (8), and the blowing module, and is used for observing and operating the instrument; The specific steps are as follows: The first step is to insert a plurality of two-dimensional planar electrodes (20) into corresponding multi-unit electromagnetic fixtures (3) respectively, and to perform corresponding functional modification on the electrodes according to the detection requirements; In the second step, solutions containing target substances of different concentrations, including test solution and detection solution, are added into the small chamber (2) of the reaction chamber integrated board (1), and are placed in the reaction chamber groove; cleaning solution is added into the cleaning pool, and is placed in the cleaning pool groove (5). The third step is to adjust parameters through the human-computer interaction module (9), including the number of cleaning times, cleaning soaking time, reaction time of each reaction chamber, reaction temperature, and blowing time; The operation process is started, and under the control of the three-dimensional driving module, the functionally modified two-dimensional planar electrode (20) array is positioned at the corresponding position of the reaction chamber groove; the two-dimensional planar electrode (20) is immersed in the solution, and reacts in each reaction chamber in sequence according to the setting; During this period, if the reaction chamber needs to be cleaned before entering a new reaction chamber, the electrode array is cleaned in the cleaning tank and positioned under the speed-adjustable fan (16) to blow out the residual liquid; The fourth step is to insert the processed planar electrode into the sensor, add the detection liquid, perform signal detection, record the data, and complete the detection process.

2. The method for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 1, characterized in that: When performing electrochemiluminescent biosensor detection, two reaction chamber grooves are provided, namely, reaction chamber groove No. 1 (4) and reaction chamber groove No. 2 (6), and the following steps are specifically included: Add test solutions containing target substances of different concentrations into the small chambers (2) of the No. 1 reaction chamber integrated board (1) one by one, and place them in the groove (4) of the No. 1 reaction chamber; add a secondary antibody solution labeled with an electrochemical luminescent material into the No. 2 reaction chamber integrated board (1), and place it in the groove (6) of the No. 2 reaction chamber; add PBST cleaning solution into a cleaning pool, divide the cleaning pool into small chambers matching the size of the multi-unit electromagnetic fixture (3), and place them in the groove (5) of the cleaning pool; The number of cleaning times, the soaking time for each cleaning, the reaction time of reaction chamber No. 1, the reaction time of reaction chamber No. 2, and the reaction temperature blowing time are regulated by the human-computer interaction module (9) to start the operation process; The two-dimensional planar electrode (20) chip array is positioned at the corresponding position of the groove (4) of the No. 1 reaction chamber, completely immersed in the solution to be tested, reacted in an environment of 37° C., and the target is captured on the electrode surface; thereafter, the electrode array is positioned under a speed-adjustable fan (16) to blow out the residual liquid; after cleaning, the electrode array is positioned at the groove (6) of the No. 2 reaction chamber, reacted in an environment of 37° C., and the electrochemiluminescent labeled secondary antibody is combined with the target to construct a double antibody sandwich structure; After cleaning and drying, the treated planar electrode is inserted into the electrochemiluminescence sensor, and the detection liquid is added, and the electrochemiluminescence signal is detected, and the data is recorded to complete the detection process.

3. The method for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 2, characterized in that: The two-dimensional planar electrode (20) for electrochemiluminescent biosensor detection is subjected to the following functional modification treatment: A dopamine potassium carbonate buffer solution was prepared and the pH value was adjusted to 9.6, and a saturated concentration of nanomaterials was added thereto, and the nanomaterials were evenly dispersed and added to the No. 1 reaction chamber integrated board; The target-specific antibody solution is added to the No. 2 reaction chamber integrated board and placed in the No. 2 reaction chamber groove (4); PBST cleaning solution is added to the cleaning pool, and the cleaning pool is divided into small chambers matching the size of the multi-unit electromagnetic fixture (3), which are placed in the cleaning pool groove (5). The electrode array is assembled, and the parameters are adjusted through the human-computer interaction module (9) to start the operation process; the electrode chip array is carried in the multi-unit electromagnetic clamp (3) to the corresponding position of the groove (4) of the No. 1 reaction chamber, and is completely immersed in the mixed solution of nanomaterials and dopamine to react, and the nanomaterials are fixed on the electrode surface through the formation of polydopamine; the electrode array is driven under the speed-adjustable fan (16) to quickly blow out the residual liquid; after blowing away the residual liquid, the cleaning operation is performed according to the set cleaning and soaking, and the residual liquid must be blown away after each soaking; After cleaning, the electrode array is driven to the groove (6) of the No. 2 reaction chamber and immersed for a set time to combine the antibody with polydopamine through a covalent reaction to achieve functional modification of the antibody on the electrode surface.

4. A device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier as described in claim 1.

5. The device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 4, characterized in that: The device is provided with a housing, and a human-machine interaction module (9) is located on the side of the housing of the device and is used for an operator to observe parameters and operate the device.

6. The device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 4, characterized in that: The solution capacity of the small chambers (2) of the reaction chamber integrated plate (1) is 50 to 500 microliters, and the integrated number is 5 to 20.

7. The device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 4, characterized in that: The three-dimensional driving module comprises an X-axis servo motor (10), a Y-axis servo motor guide rail (11), a Z-axis servo motor (12), a Z-axis servo motor guide rail (13), an X-axis servo motor guide rail (14), and a Y-axis servo motor (15); a multi-unit electromagnetic fixture (3) is fixed to the end of the Z-axis servo motor guide rail (13), the Z-axis servo motor (12) and the Z-axis servo motor guide rail (13) are located in the Y-axis servo motor guide rail (11), and the X-axis servo motor guide rail (14) and the Y-axis servo motor guide rail (11) are arranged vertically; and the multi-unit electromagnetic fixture (3) and the two-dimensional sensor chip (20) therein can be moved arbitrarily inside the device under the drive of the multiple servo motors.

8. The device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 4, characterized in that: The temperature control range of the heating and temperature control module (8) is 20-100°C.

9. The device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 4, characterized in that: The number of grooves in the groove module is 3 to 8.

10. The device for improving the biological detection efficiency and throughput of a two-dimensional sensing carrier according to claim 4, characterized in that: The reaction chamber integrated plate (1) is formed by composite nesting or integral molding of independent small chambers (2) of the same specifications.

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