Deer skin glue product and processing technology thereof

Abstract

The invention discloses a deer skin gelatin product and a processing technology thereof, and relates to the field of health food, the deer skin gelatin product comprises the following raw materials by weight: 600% of dehaired deer skin, 15% of rock sugar, 10% of yellow wine, 12% of soybean oil, 7% of powdery phospholipid, 5% of a cordyceps sinensis extract, 8% of a ganoderan extract, and 3% of selenoprotein peptide. The components have definite immunoregulation, anti-oxidation and anti-stress capabilities, the pharmacological action of the deer skin gelatin is remarkably enhanced, high-pressure boiling extraction and multiple concentration technologies are used, the extraction rate of effective components in the deer skin gelatin is increased, and the quality and stability of the product are remarkably improved.

Description

Technical Field

[0001] The present application relates to the field of health food, and in particular to deerskin glue products and their processing technology. Background Art

[0002] Deerskin glue, which involves the field of health food, is a traditional animal glue product made from deerskin, and its main component is collagen. As a tonic, deerskin glue has high nutritional value and is believed to have the effects of nourishing blood and qi, strengthening tendons and bones, etc. However, the preparation process and ingredient functionality of traditional deerskin glue have certain technical limitations.

[0003] For example, Chinese invention patent CN1907126A discloses a deerskin glue product and its processing technology, which is made of raw materials and methods with the following weight proportions: a. first, soak the deerskin with deer hair removed in alkaline water with pH = 10-11 for 20-48 hours, and then wash it with water until it is neutral to obtain clean deerskin; b. Add water to the clean deerskin in step a and boil it at a pressure of 0.15-0.4MPa for 0.5-4 hours, filter it, and concentrate the filtrate to obtain deerskin glue liquid; c. According to the ratio of clean deerskin: rock sugar: rice wine, soybean oil: powdered lecithin = 600:12-18:4-12:12-15:6-8, add rock sugar, rice wine, soybean oil, and powdered lecithin to the deerskin glue liquid in step b, and then inject mold, gel, air-dry, suffocate, and wipe it to dry. However, the existing deerskin glue raw materials are difficult to identify, the production process has problems, and the pharmacological effects are inaccurate. Summary of the invention

[0004] The purpose of this application is to provide a deerskin glue product and its processing technology, which can enhance the immune function of the human body while solving existing difficulties and problems.

[0005] The deerskin glue product is characterized in that it contains the following raw materials in weight percentage: dehaired deerskin: 600, crystal sugar: 15, rice wine: 10, soybean oil: 12, powdered lecithin: 7, cordyceps extract: 5, ganoderma lucidum polysaccharide extract: 8, selenoprotein peptide: 3. The cordyceps extract and ganoderma lucidum polysaccharide are added. These two ingredients have clear immunomodulatory and antioxidant effects. The addition of selenoprotein peptide further enhances immunity and improves the body's anti-stress ability.

[0006] The processing technology of the deerskin glue product provided in the present application comprises the following steps: S1, placing the deerskin with deer hair removed in alkaline water with a pH value of 10.5 and soaking it for 36 hours, wherein the alkaline water is prepared by mixing sodium carbonate and sodium hydroxide in a ratio of 1:1; S2. After soaking, wash with clean water until it becomes neutral to obtain clean deerskin; S3, add clean deerskin and water in a ratio of 1:5 into a pressure cooker, cook at a pressure of 0.3 MPa for 2 hours, collect the filtrate and repeat twice; S4, after combining all the filtrates, concentrate under reduced pressure until the solid content reaches 20%-30%, to obtain deerskin glue; S5, adding crystal sugar, rice wine, soybean oil, powdered lecithin, cordyceps extract, ganoderma lucidum polysaccharide extract, and selenoprotein peptide to the concentrated deerskin glue in a ratio of 2-3:1-2:2-2.5:1-1.5:0.5-1:1-1.5:0.1-0.6, stirring evenly, and maintaining the temperature at 60-70° C. to avoid inactivation of the components; S6, injecting the mixed deerskin glue into the mold, and cooling it to room temperature to form a gel; S7. After demoulding the gel, place it in a clean environment to dry the gel, keep the temperature at 20-30°C, and control the humidity at about 60% for 48 hours; S8, drying the glue-dried product at 40°C until the moisture content is less than 10%; S9. After drying, cut into appropriate sizes and seal and package.

[0007] Preferably, the S4 is concentrated under reduced pressure until its solid content reaches 25%.

[0008] Preferably, in S5, in the original order, the ratio of each substance is 2.5:1.7:2:1.2:0.8:1.3:0.5.

[0009] Preferably, the temperature in S5 is maintained at 65°C.

[0010] Preferably, the temperature in S7 is maintained at 25°C.

[0011] Preferably, each substance in S5 is added one by one during the stirring process to ensure uniform mixing.

[0012] Compared with the prior art, the advantages of the present invention are: (1) The present invention adds Cordyceps extract, Ganoderma lucidum polysaccharide and selenoprotein peptide, which have clear immunomodulatory, antioxidant and anti-stress capabilities and significantly enhance the pharmacological effects of deerskin glue.

[0013] (2) The present invention uses high-pressure boiling and multiple concentration technology to improve the extraction rate of effective ingredients in deerskin glue and significantly improve the quality and stability of the product.

[0014] (3) Clear quantitative labeling of the functional ingredients in deerskin glue ensures the controllability and consistency of the product's efficacy, solving the problem of unclear pharmacological effects of traditional deerskin glue.

[0015] (4) The improved extraction and concentration process of the present invention reduces resource waste, improves the utilization rate of raw materials, and is more environmentally friendly and efficient. DETAILED DESCRIPTION

[0016] The present application is further described in detail below in conjunction with examples, comparative examples and performance testing experiments. These examples should not be construed as limiting the scope of protection claimed in the present application. Example

[0017] Example 1: Deerskin glue product, which contains the following raw materials in weight percentage: dehaired deerskin: 600, crystal sugar: 15, rice wine: 10, soybean oil: 12, powdered lecithin: 7, cordyceps extract: 5, ganoderma lucidum polysaccharide extract: 8, selenoprotein peptide: 3.

[0018] The processing technology of deerskin glue products includes the following steps: S1, placing the deerskin with deer hair removed in alkaline water with a pH value of 10.5 and soaking it for 36 hours, wherein the alkaline water is prepared by mixing sodium carbonate and sodium hydroxide in a ratio of 1:1; S2. After soaking, wash with clean water until it becomes neutral to obtain clean deerskin; S3, add clean deerskin and water in a ratio of 1:5 into a pressure cooker, cook at a pressure of 0.3 MPa for 2 hours, collect the filtrate and repeat twice; S4, after combining all the filtrates, concentrate under reduced pressure until the solid content reaches 20%-30%, to obtain deerskin glue; S5, adding crystal sugar, rice wine, soybean oil, powdered lecithin, cordyceps extract, ganoderma lucidum polysaccharide extract, and selenoprotein peptide to the concentrated deerskin glue in a ratio of 2.5:1.7:2:1.2:0.8:1.3:0.5, stirring evenly, and maintaining the temperature at 65° C. to avoid inactivation of the components; S6, injecting the mixed deerskin glue into the mold, and cooling it to room temperature to form a gel; S7, after demoulding the gel, place it in a clean environment to dry the gel, keep the temperature at 25°C and the humidity at about 60% for 48 hours; S8, drying the glue-dried product at 40°C until the moisture content is less than 10%; S9. After drying, cut into appropriate sizes and seal and package.

[0019] Example 2: The difference from Example 1 is that in the preparation process S5, the Ganoderma lucidum polysaccharide extract is increased to 2%, and the rice wine is reduced to 1%, and the other ingredients remain unchanged. The original proportion is changed to rock sugar, rice wine, soybean oil, powdered lecithin, cordyceps extract, Ganoderma lucidum polysaccharide extract, and selenoprotein peptide, which are added to the concentrated deerskin glue in a ratio of 2.5:1:2:1.2:0.8:2:0.5.

[0020] Example 3: The difference from Example 1 is that in the preparation process S3, the extraction pressure of the deerskin is increased from 0.3 MPa to 0.4 MPa, and the extraction time is shortened from 2 hours to 1 hour.

[0021] Example 4: The difference from Example 1 is that in the preparation process S5, the selenoprotein peptide content is reduced to 0.2%, and the proportions of other ingredients remain unchanged in Example 1, and the original proportions are changed to rock sugar, rice wine, soybean oil, powdered lecithin, cordyceps extract, ganoderma lucidum polysaccharide extract, and selenoprotein peptide in a ratio of 2.5:1:2:1.2:0.8:2:0.2, which are added to the concentrated deerskin glue.

[0022] Example 5: The difference from Example 1 is that in the preparation process S5, the cordyceps extract is increased to 1.5%, and the rice wine is reduced to 0.5%. The other ingredients are the same as in Example 1, and the original proportions are changed to rock sugar, rice wine, soybean oil, powdered lecithin, cordyceps extract, ganoderma polysaccharide extract, and selenoprotein peptides, which are added to the concentrated deerskin glue in a ratio of 2.5:0.5:2:1.2:1.5:2:0.5.

[0023] Example 6: The difference from Example 1 is that in the preparation process S3, the extraction pressure is increased to 0.5 MPa, the extraction time is 1.5 hours, and the other steps are the same as Example 1.

[0024] Example 7: The difference from Example 1 is that in the preparation process S5, the Ganoderma lucidum polysaccharide extract is increased to 1.5%, the Cordyceps sinensis extract is reduced to 1%, the selenoprotein peptide is increased to 0.8%, and the crystal sugar is reduced to 2%. The other ingredients remain unchanged in the proportions of Example 1, and the original proportions are changed to crystal sugar, rice wine, soybean oil, powdered lecithin, Cordyceps sinensis extract, Ganoderma lucidum polysaccharide extract, and selenoprotein peptide are added to the concentrated deerskin glue in a ratio of 2:1:2:1.2:1:1.5:0.8. In the preparation process S3, the extraction pressure is 0.35 MPa, and the time is 1.5 hours. The other steps are the same as in Example 1.

[0025] Comparative Example 1: The difference from Example 1 is that the raw material ratio is based on the background scheme and the background process is adopted.

[0026] Comparative Example 2 is different from Example 1 in that the Ganoderma lucidum polysaccharide extract is removed based on Example 1.

[0027] Comparative Example 3: The difference from Example 1 is that the Cordyceps extract is removed on the basis of Example 1, and other ingredients remain unchanged.

[0028] Now, a comparative experiment is conducted on Examples 1-7 and Comparative Examples 1-3 to test the differences in sample stability, immune function, taste and functionality. Three experiments are conducted respectively, and the test steps are as follows: Experiment 1: Physical and Chemical Analysis Step 1: Detect key ingredients: collagen peptide, Ganoderma lucidum polysaccharide, Cordyceps polysaccharide, selenoprotein peptide content; Step 2: quantitative analysis using high performance liquid chromatography and ultraviolet spectrophotometer; Step 3: Store the samples at 25°C and 60% humidity for 1 month to evaluate the stability of the samples.

[0029] Experiment 2: Immune function test Step 1, 50 healthy mice were selected and randomly divided into 10 groups, and the mice were respectively administered with the samples of the embodiment and the comparative example at a dose of 1 g / kg body weight, once a day for 30 consecutive days; Step 2: Use flow cytometry to detect the number and subpopulation distribution of leukocytes, use ELISA to detect the levels of serum immunoglobulins IgA, IgG, and IgM, and use carbon particle clearance method to detect the phagocytic ability of macrophages.

[0030] Experiment 3: Sensory evaluation test Step 1: A 10-member sensory evaluation panel will evaluate the sample's color, smell, taste and solubility, with each item scoring out of 10 points.

[0031] The experimental results are as follows: The results of physical and chemical analysis are shown in Table 1: Table 1 The results of immune function test are shown in Table 2: Table 2 The sensory evaluation test results are shown in Table 3: Group Color odor Taste Solubility Overall rating Example 1 8.5 8.8 9.2 9.0 35.5 Example 2 9.0 9.0 9.5 9.2 36.7 Example 3 8.7 8.9 9.3 9.1 36.0 Example 4 8.3 8.5 9.0 8.8 34.6 Example 5 8.8 8.8 9.4 9.2 36.2 Example 6 9.0 9.1 9.6 9.3 36.9 Example 7 9.2 9.3 9.7 9.4 37.6 Comparative Example 1 6.5 6.8 7.0 7.2 27.5 Comparative Example 2 7.5 7.8 8.0 8.0 31.3 Comparative Example 3 7.8 8.0 8.2 8.5 32.5 Table 3 In terms of physical and chemical analysis, the contents of key functional ingredients in Examples 1-7 were significantly higher than those in the comparative examples. The Ganoderma polysaccharide in the comparative example 2 lacking Ganoderma polysaccharide could not be detected, while other ingredients remained normal. The content of key ingredients in Example 7 was higher as a whole, especially Ganoderma polysaccharide and selenoprotein peptide, which showed the advantages of the comprehensive optimization scheme and verified the functional source of Ganoderma polysaccharide. The Cordyceps polysaccharide in the comparative example 3 could not be detected, verifying the importance of Cordyceps extract to functionality.

[0032] In terms of immune function testing, the example groups significantly improved the total white blood cell count, immunoglobulin content and macrophage phagocytic ability. The immune enhancement effect of Example 7 was the most significant, and the data of white blood cells, IgG and macrophage phagocytic ability were better than those of other groups, indicating that the increase of Ganoderma lucidum polysaccharides helps to further enhance the immune function. The immune enhancement effect of Example 1 was the lowest, indicating that the functional ingredients added in the improved scheme had a significant immune enhancement effect, while the immune enhancement effect of Example 4 was relatively low, indicating that the reduction in selenoprotein peptide content has a greater impact on the immune function.

[0033] In terms of taste and functionality, the overall scores of Examples 1-7 are higher than those of Comparative Examples 1-3, especially in terms of taste and solubility. Comparative Example 2 has a slightly lower score due to the lack of Ganoderma lucidum polysaccharide, indicating that the addition of Ganoderma lucidum polysaccharide has an effect on improving the sensory properties. Example 7 has the highest comprehensive sensory score, indicating that the synergistic effect of optimizing the formula and process has significantly improved the sensory properties.

[0034] The functional components in deerskin glue need to be efficiently absorbed and utilized by the human body to truly play a role. However, absorption rate and bioavailability are the shortcomings of current deerskin glue. Now, a new comparative experiment is conducted on the above-mentioned embodiment and comparative example to verify the difference in their effects in these aspects. The experimental steps are as follows: Experiment 1: In vitro simulated digestion experiment Step 1: Prepare simulated gastric juice and small intestinal juice. The gastric juice is 0.1M HCl solution, and 1.0g / L of pepsin is added; the small intestinal juice is phosphate buffer, and 1.5g / L of pancreatin is added; Step 2, each example, comparative example and control group sample was dissolved in simulated gastric juice, shaken in a water bath at 37° C. for 2 hours, and the sample after gastric juice degradation was collected, and the functional component content was measured by HPLC; Step 3: Transfer the sample treated with gastric juice to simulated small intestinal fluid, shake it in a 37°C water bath for 4 hours, use a dialysis bag model to simulate intestinal absorption, and measure the membrane permeability of the functional components in the sample in the dialysis bag.

[0035] Experiment 2: Animal Experiment Step 1: Use healthy rats, 6 rats in each group, and divide them into the following groups: Examples 1-7, Comparative Examples 1-3, and Control Group; Step 2: Each group of rats were gavaged and the sample concentrations were standardized, with collagen peptide at 100 mg / mL, Ganoderma lucidum polysaccharide at 10 mg / mL, and selenoprotein peptide at 5 mg / mL; Step 3, blood samples were collected from the rat tail vein at 0.5 h, 1 h, 2 h, 4 h, 8 h, and 12 h, and the plasma samples were processed and the functional component concentrations were measured by LC-MS; Step 4: Calculate the blood drug concentration-time curve and estimate the bioavailability by the AUC value.

[0036] Experiment 3: Experiment on the effect of intestinal flora metabolism Step 1: Use healthy rats, 6 rats per group, the grouping is the same as the animal experiment; Step 2: Each group was gavaged with deerskin glue samples for 14 consecutive days, and fecal samples were collected on the last day; Step 3: Extract fecal flora DNA and perform 16S rRNA sequencing to analyze flora diversity; Step 4: Measure the concentration of short-chain fatty acids in fecal metabolites using GC-MS analysis.

[0037] The experimental results are as follows: The results of the in vitro simulated digestion experiment are shown in Table 4: Group Gastric juice degradation rate Small intestinal permeability Example 1 15.0 35.0 Example 2 12.5 38.5 Example 3 14.0 36.0 Example 4 18.0 32.5 Example 5 12.0 40.0 Example 6 11.0 42.5 Example 7 10.5 45.0 Comparative Example 1 25.0 20.0 Comparative Example 2 22.0 25.0 Comparative Example 3 18.0 28.0 Control group (collagen peptide) 5.0 50.0 Table 4 The results of animal experiments are shown in Table 5: Table 5 The experimental results of the effect of intestinal flora metabolism are shown in Table 6: Table 6 In terms of improving absorption efficiency, the membrane permeability, bioavailability and concentration of bacterial metabolites of the functional ingredients in Example 7 are the highest, indicating that its optimized scheme significantly promotes absorption. The absorption rate of Comparative Example 1 is the lowest, verifying the shortcomings of the background scheme. In terms of the bacterial flora regulation effect, Example 7 has high concentrations of Ganoderma lucidum polysaccharides and selenoprotein peptides, which can significantly regulate bacterial flora diversity and SCFA production, and the interaction mechanism between specific components and intestinal flora can be further studied.

[0038] The basic principles, main features and advantages of the present invention are shown and described above; those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected; the scope of protection claimed by the present invention is determined by the attached claims and their effects.

Claims

1. Deerskin glue product, characterized in that: It contains the following raw materials in weight percentage: dehaired deerskin: 600, crystal sugar: 15, rice wine: 10, soybean oil: 12, powdered lecithin: 7, cordyceps extract: 5, ganoderma lucidum polysaccharide extract: 8, selenoprotein peptide:

3.

2. A processing technology for deerskin glue products, which relates to the deerskin glue products according to claim 1, characterized in that: The following steps are involved: S1. Soak the deerskin with deer hair removed in alkaline water with a pH value of 10.5 for 36 hours; S2. After soaking, wash with clean water until it becomes neutral to obtain clean deerskin; S3, add clean deerskin and water in a ratio of 1:5 into a pressure cooker, cook at a pressure of 0.3 MPa for 2 hours, collect the filtrate and repeat twice; S4, after combining all the filtrates, concentrate under reduced pressure until the solid content reaches 20%-30%, to obtain deerskin glue; S5, adding crystal sugar, rice wine, soybean oil, powdered lecithin, cordyceps extract, ganoderma lucidum polysaccharide extract, and selenoprotein peptide to the concentrated deerskin glue in a ratio of 2-3:1-2:2-2.5:1-1.5:0.5-1:1-1.5:0.1-0.6, stirring evenly, and maintaining the temperature at 60-70°C; S6, injecting the mixed deerskin glue into the mold, and cooling it to room temperature to form a gel; S7. After demoulding the gel, place it in a clean environment to dry the gel, keep the temperature at 20-30°C, and control the humidity at about 60% for 48 hours; S8, drying the glued product at 40°C until the moisture content is less than 10%; S9. After drying, cut into appropriate sizes and seal and package.

3. The processing technology of deerskin glue products according to claim 2 is characterized in that: The S4 is concentrated under reduced pressure until its solid content reaches 25%.

4. The processing technology of deerskin glue products according to claim 2, characterized in that: In S5, in the original order, the ratio of each substance is 2.5:1.7:2:1.2:0.8:1.3:0.

5.

5. The processing technology of deerskin glue products according to claim 2, characterized in that: In S5, the temperature is maintained at 65°C.

6. The processing technology of deerskin glue products according to claim 2, characterized in that: In S7, the temperature is maintained at 25°C.

7. The processing technology of deerskin glue products according to claim 2, characterized in that: During the stirring process of S5, each substance should be added one by one to ensure uniform mixing.

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