Exosome detection device

Abstract

The invention discloses an exosome detection device, and belongs to the technical field of exosome detection. The exosome detection device comprises a stirring cylinder and further comprises stirring parts, the stirring parts are arranged in the stirring cylinder and comprise vertically-arranged rotating rods and driving parts connected to the top ends of the rotating rods, the rotating rods are hollow, a plurality of stirring rods are obliquely inserted into the rotating rods in a sliding mode, pulling parts are arranged in the rotating rods, and the pulling parts are arranged on the rotating rods. The stirring device comprises a stirring barrel, a rotating rod is arranged in the stirring barrel, a pulling piece is arranged in the stirring barrel and comprises a vertically-arranged pull rod, a plurality of stirring rods are hinged to the pull rod through hinge rods, a scraping piece is arranged in the stirring barrel and comprises a horizontally-arranged scraping disc, and the top of the scraping disc is connected with a pushing drive. And the stirring liquid attached to the rotating rod is scraped off through the scraping piece, so that the situation that when the stirring liquid obtained after stirring is completed is guided out, a large error is generated between the stirring liquid and needed discharging is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of exosome detection, and in particular to a device for detecting exosomes. Background Art

[0002] Tumor diagnosis and prediction usually involve testing whether the RNA content of exosomes is normal. Ultraviolet absorption is a method of RNA detection. The principle is that the purine and pyrimidine bases in the molecular structure of nucleic acids, nucleotides and their derivatives have a conjugated double bond system, which can strongly absorb ultraviolet light with a wavelength of 250nm-280nm. The maximum ultraviolet absorption value of nucleic acids is at 260nm. According to the Lambert-Beer law, the content of nucleic acid substances can be detected from the change in ultraviolet absorption value. The tautomerism of purine and pyrimidine bases in different pH solutions is different, and the ultraviolet absorption light also shows obvious differences, and their molar extinction coefficients are also different.

[0003] During the sample processing operation, it is necessary to weigh 0.2g-0.25g crude RNA, add 2ml, 0.2% NaOH solution and 1ml H2O solution, mix into a paste, then add 40-50ml H2O to dissolve, adjust the PH to 7.0, and then make the volume 100ml. Usually, when the sample is modulated, the stirring rod or stirring plate is driven by a motor to stir the mixture. However, during the stirring process, because the mixed liquid is in a paste state and the stirring rod is fixed, part of the stirring liquid will stick to the stirring rod and the inner wall of the stirring barrel. When the stirring liquid is exported after the stirring is completed, the exported stirring liquid will be different from the amount of stirring liquid required, which will affect the accuracy of subsequent detection. Summary of the invention

[0004] The purpose of the present invention is to overcome the problems in the prior art and provide an exosome detection device to strengthen the discharge and reduce the large error generated when the stirring liquid is discharged after the stirring is completed.

[0005] The present invention provides an exosome detection device, comprising a stirring drum and a stirring member, wherein the stirring member is arranged in the stirring drum, the stirring member comprises a vertically arranged rotating rod and a driving member connected to the top end of the rotating rod, the rotating rod is hollow, and a plurality of stirring rods are obliquely and slidably inserted on the rotating rod, a pulling member is arranged in the rotating rod, the pulling member comprises a vertically arranged pulling rod, the pulling rod coincides with the axis of the rotating rod, and a plurality of the stirring rods are hinged to the pulling rod through a hinged rod, a scraping member is arranged in the stirring drum, the scraping member comprises a horizontally arranged scraping disk, the scraping disk is slidably sleeved on the rotating rod, and the scraping disk is arranged in contact with the inner side wall of the stirring drum, and a propulsion drive is connected to the top of the scraping disk.

[0006] Preferably, the pulling member also includes a first cylinder, which is vertically arranged in the rotating rod, the output end of the first cylinder is fixedly connected to the bottom end of the pulling rod, the top end of the pulling rod is fixedly connected to the inner side of the rotating rod through a telescopic rod, one end of the hinged rod is hinged to the pulling rod, and the plurality of stirring rods have sufficient spacing from each other in the vertical direction.

[0007] Preferably, the stirring rod is arranged in an arc shape away from one end of the rotating rod, and a slot is provided in the sliding area of ​​the stirring rod and the rotating rod. When the stirring rod is placed inside the rotating rod, the end of the stirring rod is confined in the slot, and the end of the stirring rod is completely matched with the slot.

[0008] Preferably, the driving member is configured as a motor, the motor is vertically disposed on the top of the mixing drum, and the output end of the motor passes through the mixing drum and is fixedly connected to the top end of the rotating rod.

[0009] Preferably, a bottom cover is provided at the bottom of the mixing drum, and a feeding pipe is vertically provided at the top of the mixing drum.

[0010] Preferably, the propulsion drive is configured as a second cylinder, the second cylinder is vertically disposed on the top of the mixing drum, an arc block is fixedly connected to the output end of the second cylinder, the arc block is rotatably embedded in the scraper disk, and the feeding pipe slides through the scraper disk.

[0011] Preferably, a connecting piece is provided between the scraper disk and the rotating rod, the connecting piece includes a support ring, the support ring is arranged on the top of the rotating rod and coincides with the axis of the rotating rod, the rotating rod is slidably inserted with a rotating rod, the fixed rod is fixedly connected to the bottom of the support ring, the bottom of the support ring is provided with multiple groups of telescopic limit members, the scraper disk is provided with a limit groove matching the limit disk, the fixed rod is provided with a first spring, one end of which is fixedly connected to the support ring, and the other end is fixedly connected to the rotating rod.

[0012] Preferably, the telescopic limit member includes a support tube, which is vertically fixedly connected to the bottom of the support ring, and a limit rod is vertically slidably inserted in the support tube, the limit rod and the limit groove match each other, the limit rod is connected to the support tube through a second spring, and when the second spring is in its original state, the limit rod is inserted in the limit groove.

[0013] Preferably, a movable plate is slidably disposed in the bottom cover, a through hole is opened at the bottom of the bottom cover, and a push rod is further provided, and the push rod is threadedly embedded in the bottom of the movable plate.

[0014] Preferably, a storage box is provided on the mixing drum, and a scraper is provided inside the storage box.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: in an exosome detection device of the present invention, the rotating rod and the stirring rod are set to be adjustable. After the stirring work is completed, the stirring rod is retracted into the rotating rod, and then the stirring liquid attached to the rotating rod is scraped off by the scraper to increase the discharge amount of the stirring liquid after stirring and prevent a large error from occurring with the required discharge. The scraper is arranged to scrape off the stirring liquid attached to the rotating rod on the one hand, and to scrape off the stirring liquid attached to the inner wall of the stirring drum when moving downward on the other hand, thereby exporting the stirring liquid attached to the inner wall of the stirring drum, the stirring rod and the rotating rod. The exosome detection device of the present invention is easy to use and has strong popularization. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall first-viewing angle structure of the present invention.

[0017] Figure 2 It is a schematic diagram of the structure of the scraping member and the connecting member of the present invention.

[0018] Figure 3 It is a schematic diagram of the structure of the stirring member and the pulling member of the present invention.

[0019] Figure 4 It is a schematic diagram of the card slot structure of the present invention.

[0020] Figure 5 It is a schematic diagram of the overall second viewing angle structure of the present invention.

[0021] Figure 6 It is a schematic diagram of the structure of the mobile disk of the present invention.

[0022] Explanation of the accompanying drawings: 1. mixing drum; 2. bottom cover; 3. stirring member; 31. rotating rod; 32. motor; 33. stirring rod; 4. pulling member; 41. pulling rod; 42. hinged rod; 43. first cylinder; 44. telescopic rod; 5. scraping member; 51. scraping disk; 52. second cylinder; 53. arc block; 6. slot; 7. feeding pipe; 8. connecting member; 81. supporting ring; 82. fixing rod; 83. limiting groove; 84. first spring; 85. telescopic limiting member; 851. supporting tube; 852. second spring; 853. limiting rod; 9. movable disk; 10. pushing rod; 11. through hole; 12. storage box; 13. scraper. DETAILED DESCRIPTION

[0023] The following is combined with Figure 1 to Figure 6In order to make the purpose, technical solution and advantages of the embodiment of the present invention clearer, the technical solution of the embodiment of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiment of the present invention. Obviously, the described embodiment is a part of the embodiment of the present invention, not all of the embodiments. Based on the described embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein should be the usual meanings understood by people with general skills in the field to which the present invention belongs.

[0024] The words "first", "second" and similar words used in the patent specification and claims of the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, but do not exclude other elements or objects. "Inside", "outside", "upper", "lower", "far", "near", "front", "back" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly. The drawings in the present invention are not drawn strictly according to the actual scale. The specific size and quantity of each structure can be determined according to actual needs. The drawings described in the present invention are only schematic diagrams of the structure.

[0025] The present invention provides a detection device for exosomes, such as Figure 1 to Figure 4 As shown, it includes a mixing drum 1 and a stirring member 3. The stirring member 3 is arranged in the mixing drum 1. The stirring member 3 includes a vertically arranged rotating rod 31 and a driving member connected to the top of the rotating rod 31. The rotating rod 31 is hollow, and a plurality of stirring rods 33 are obliquely and slidably inserted on the rotating rod 31. A pulling member 4 is provided in the rotating rod 31. The pulling member 4 includes a vertically arranged pull rod 41. The pull rod 41 coincides with the axis of the rotating rod 31. The plurality of stirring rods 33 are hinged to the pull rod 41 through a hinge rod 42. A scraper 5 is provided in the mixing drum 1. The scraper 5 includes a horizontally arranged scraper disk 51. The scraper disk 51 is slidably sleeved on the rotating rod 31, and the scraper disk 51 is arranged to fit the inner wall of the mixing drum 1. The top of the scraper disk 51 is connected with a propulsion drive.

[0026] In the present invention, the rotating rod 31 and the stirring rod 33 are set to be adjustable. After the stirring work is completed, the stirring rod 33 is retracted, and then the stirring liquid attached to the rotating rod 31 is scraped off by the scraper 5 to prevent the stirring liquid from being discharged after the stirring is completed, resulting in a large error with the required discharge. When the present invention is used, the raw materials to be proportioned are added into the stirring drum 1 through the feeding pipe 7 set on the top of the stirring drum 1, and the motor 32 is turned on. The motor 32 will drive the rotating rod 31 to rotate. A plurality of stirring rods 33 are arranged on the mixing drum 1 to stir and mix the raw materials in the mixing drum 1, and under the action of the connecting member 8, the scraping disc 51 is connected with the rotating rod 31 at first, and the scraping disc 51 rotates together with the rotating rod 31. An arc scraper 13 can be arranged at the bottom of the scraping disc 51, and the arc scraper 13 is made to fit the inner wall of the mixing drum 1. When the raw materials in the mixing drum 1 are stirred by the stirring rod 33, the scraper 13 can scrape the raw materials attached to the inner wall of the mixing drum 1, so that various raw materials can be better mixed;

[0027] When the mixing work is completed and needs to be discharged, the bottom cover 2 at the bottom of the mixing drum 1 is first opened for preliminary discharging. At this time, under the push of the first cylinder 43 set inside the rotating rod 31, the set pull rod 41 will move up and press the telescopic rod 44, and the upward pull rod 41 will drive the connecting rod to drive the scraper plate 51 to separate the rotating rod 31, and the pull rod 41 will pull the mixing rod 33 through the hinge rod 42 when moving up, and pull several mixing rods 33 back to the rotating rod 31, and the end of the mixing rod 33 is completely matched with the rotating rod 31. When the mixing rod 33 is pulled back into the rotating rod 31, the mixing liquid attached to the mixing rod 33 is scraped off and blocked on the rotating rod 31. At this time, under the push of the second cylinder 52, the scraper plate 51 moves down to scrape off the mixing liquid attached to the inner wall of the mixing drum 1 and the rotating rod 31, so as to prevent the error caused during discharging from being too large and affecting the accuracy of subsequent detection.

[0028] Preferably, Figure 1 to Figure 6As shown, the pulling member 4 also includes a first cylinder 43, the first cylinder 43 is vertically arranged in the rotating rod 31, the output end of the first cylinder 43 is fixedly connected to the bottom end of the pull rod 41, the top of the pull rod 41 is fixedly connected to the inner side of the rotating rod 31 through the telescopic rod 44, one end of the hinged rod 42 is hinged to the pull rod 41, and a plurality of stirring rods 33 have a sufficient distance from each other in the vertical direction, the stirring rod 33 is arranged in an arc shape away from one end of the rotating rod 31, and a slot 6 is provided in the sliding area between the stirring rod 33 and the rotating rod 31, when the stirring rod 33 is placed inside the rotating rod 31, the end of the stirring rod 33 The part is located in the slot 6, and the end of the stirring rod 33 is completely matched with the slot 6. The driving part is set as a motor 32, and the motor 32 is vertically set at the top of the stirring drum 1. The output end of the motor 32 passes through the stirring drum 1 and is fixedly connected with the top of the rotating rod 31. A bottom cover 2 is buckled at the bottom of the stirring drum 1, and a feeding pipe 7 is vertically set at the top of the stirring drum. The propulsion drive is set as a second cylinder 52, and the second cylinder 52 is vertically set at the top of the stirring drum 1. The output end of the second cylinder 52 is fixedly connected with an arc block 53, and the arc block 53 is rotatably embedded in the scraping disk 51, and the feeding tube 7 slides through the scraping disk 51.

[0029] When the mixing work is completed and it is necessary to discharge the materials, the bottom cover 2 at the bottom of the mixing drum 1 is first opened for preliminary discharge. At this time, the pull rod 41 is pushed upward by the first cylinder 43 arranged inside the rotating rod 31, and the telescopic rod 44 is pressed. The upward pull rod 41 drives the connecting rod to separate the scraper plate 51 from the rotating rod 31, and the pull rod 41 pulls the stirring rod 33 through the hinge rod 42 when moving upward, and pulls a plurality of stirring rods 33 back into the rotating rod 31, and the ends of the stirring rods 33 are completely fitted with the rotating rod 31, and the stirring rods 33 are completely fitted with the rotating rod 31. When the stirring liquid attached to the stirring rod 33 is scraped off and blocked on the rotating rod 31 when it is pulled back to the rotating rod 31, at this time, under the push of the second cylinder 52, the scraping plate 51 moves down to scrape off the stirring liquid attached to the inner wall of the stirring drum 1 and the rotating rod 31, so as to prevent the error caused during the discharge from being too large and affecting the accuracy of subsequent detection. A clamping groove 6 is provided in the sliding area of ​​the stirring rod 33 and the rotating rod 31. When the stirring rod 33 is placed inside the rotating rod 31, the end of the stirring rod 33 is limited in the clamping groove 6, so that the end of the stirring rod 33 can better fit with the rotating rod 31.

[0030] Preferably, Figure 3 to Figure 5As shown, a connecting member 8 is provided between the scraping disc 51 and the rotating rod 31, and the connecting member 8 includes a supporting ring 81, which is arranged on the top of the rotating rod 31 and coincides with the axis of the rotating rod 31, and the rotating rod 31 is slidably inserted on the rotating rod 31, and a fixing rod 82 is fixedly connected to the bottom of the supporting ring 81, and a plurality of groups of telescopic limit members 85 are provided at the bottom of the supporting ring 81, and a limiting groove 83 matching the limiting disc is opened on the scraping disc 51, and a first spring 84 is sleeved on the fixing rod 82, and one end It is fixedly connected to the support ring 81, and the other end is fixedly connected to the rotating rod 31. The telescopic limit member 85 includes a support tube 851, and the support tube 851 is vertically fixedly connected to the bottom of the support ring 81. A limit rod 853 is vertically slidably inserted in the support tube 851. The limit rod 853 and the limit groove 83 match each other. The limit rod 853 is connected to the support tube 851 through a second spring 852, and when the second spring 852 is in the original state, the limit rod 853 is inserted in the limit groove 83.

[0031] In the present invention, in the initial state, the limiting rod 853 is inserted into the limiting groove 83. Under the action of the telescopic limiting member 85, the scraping disc 51 is connected to the rotating rod 31 through the supporting ring 81, so that the scraping disc 51 can rotate together with the rotating rod 31. When the stirring work is completed and the material needs to be discharged, under the action of the pulling member 4, as the pulling rod 41 moves upward, the fixed rod 82 is pressed, and the fixed rod 82 lifts the supporting ring 81 and pulls the first spring 84. As the supporting ring 81 moves upward, the limiting member is lifted from the limiting groove 83, thereby separating the scraping disc 51 from the rotating rod 31.

[0032] When the device is cleaned after a stirring work is completed, the pull rod 41 is pulled down through the pulling piece 4, the stirring rod 33 will extend out from the rotating rod 31 again, and the stretched first spring 84 needs to restore the original position, so the fixed rod 82 will be pulled down, so that the support ring 81 moves down and the telescopic limit member 85 is re-embedded in the limit groove 83, connecting the scraper disk 51 with the rotating rod 31, and because each rotation is completed and it cannot be guaranteed that the limit rod 853 can be accurately embedded in the limit groove 83 when pulling back, at this time, only the rotating rod 31 needs to rotate the support ring 81 to drive the set several telescopic limit members 85 to rotate, and when the limit rod 853 reaches the top of the limit groove 83, the compressed second spring 852 will push the limit rod 853 into the limit groove 83 to complete the limit.

[0033] Preferably, Figure 6 As shown, a movable plate 9 is slidably arranged in the bottom cover 2, a through hole 11 is opened at the bottom of the bottom cover 2, and a propulsion rod 10 is also provided. The propulsion rod 10 is threadedly embedded in the bottom of the movable plate 9, and a storage box 12 is provided on the mixing drum 1, and a scraper 13 is provided in the storage box 12.

[0034] Because part of the stirring liquid will be retained in the bottom cover 2, a movable plate 9 is provided on the bottom cover 2, and a propulsion rod 10 is also provided. The propulsion rod 10 is separated from the movable plate 9 during stirring. When discharging is required, the bottom cover 2 is separated from the mixing drum 1, and the propulsion rod 10 is connected to the movable plate 9. At this time, the user pushes the propulsion rod 10 to push the movable plate 9 out of the bottom cover 2, and the stirring liquid attached to the movable plate 9 is scraped and collected by the scraper 13 provided. The present invention collects the materials to the maximum extent during discharging from all aspects, thereby reducing the error caused by discharging.

[0035] The method for using the exosome detection device of the present invention is as follows:

[0036] The raw materials to be proportioned are added into the mixing drum 1 through the feeding tube 7 arranged on the top of the mixing drum 1, and the motor 32 is turned on. The motor 32 will drive the rotating rod 31 to rotate, and the raw materials in the mixing drum 1 are stirred and mixed by a plurality of stirring rods 33 arranged on the rotating rod 31. Under the action of the connecting piece 8, the scraper disk 51 is initially connected to the rotating rod 31, and the scraper disk 51 will rotate with the rotating rod 31. An arc scraper 13 can be arranged at the bottom of the scraper disk 51, and the arc scraper 13 is made to fit the inner wall of the mixing drum 1. When the raw materials in the mixing drum 1 are stirred by the stirring rod 33, the scraper 13 can scrape the raw materials attached to the inner wall of the mixing drum 1, so that various raw materials can be better mixed.

[0037] When the mixing work is completed and it is necessary to discharge the materials, the bottom cover 2 at the bottom of the mixing drum 1 is first opened for preliminary discharging. At this time, the pull rod 41 is moved upward and the telescopic rod 44 is pressed by the push of the first cylinder 43 arranged inside the rotating rod 31, and the upward pull rod 41 drives the connecting rod to drive the scraper plate 51 to separate the rotating rod 31, and the pull rod 41 will pull the mixing rod 33 through the hinge rod 42 when moving upward, and pull several mixing rods 33 back into the rotating rod 31, and the ends of the mixing rods 33 are completely matched with the rotating rod 31. When the mixing rods 33 are pulled back into the rotating rod 31, the mixing liquid attached to the mixing rod 33 is scraped off and blocked on the rotating rod 31. At this time, under the push of the second cylinder 52, the scraper plate 51 moves downward to scrape off the mixing liquid attached to the inner wall of the mixing drum 1 and the rotating rod 31, so as to prevent the error caused during discharging from being too large and affecting the accuracy of subsequent detection.

[0038] Because part of the stirring liquid will be retained in the bottom cover 2, a movable plate 9 is provided on the bottom cover 2, and a propulsion rod 10 is also provided. The propulsion rod 10 is separated from the movable plate 9 during stirring. When discharging is required, the bottom cover 2 is separated from the mixing drum 1, and the propulsion rod 10 is connected to the movable plate 9. At this time, the user pushes the propulsion rod 10 to push the movable plate 9 out of the bottom cover 2, and the stirring liquid attached to the movable plate 9 is scraped and collected by the scraper 13 provided. The present invention collects the materials to the maximum extent during discharging from all aspects, thereby reducing the error caused by discharging.

[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for detecting exosomes, comprising a mixing drum (1), characterized in that: Also includes; A stirring member (3) is arranged in the stirring drum (1), the stirring member (3) comprises a vertically arranged rotating rod (31) and a driving member connected to the top of the rotating rod (31), the rotating rod (31) being arranged hollow inside; a plurality of stirring rods (33) are arranged, and the plurality of stirring rods (33) are obliquely and slidably inserted on the rotating rod (31); A pulling member (4) is arranged in the rotating rod (31), the pulling member (4) comprises a vertically arranged pulling rod (41), the axis of the pulling rod (41) coincides with the axis of the rotating rod (31), and a plurality of the stirring rods (33) are hinged to the pulling rod (41) via a hinge rod (42); A scraper (5) is arranged in the mixing drum (1), and the scraper (5) comprises a horizontally arranged scraper disc (51), the scraper disc (51) is slidably sleeved on the rotating rod (31), and the scraper disc (51) is arranged in close contact with the inner wall of the mixing drum (1); and a propulsion drive is connected to the top of the scraper disc (51).

2. The exosome detection device according to claim 1, characterized in that: The pulling member (4) also includes a first cylinder (43), which is vertically arranged in the rotating rod (31), and the output end of the first cylinder (43) is fixedly connected to the bottom end of the pulling rod (41), and the top end of the pulling rod (41) is fixedly connected to the inner side of the rotating rod (31) through a telescopic rod (44), one end of the hinged rod (42) is hinged to the pulling rod (41), and a plurality of the stirring rods (33) have sufficient spacing from each other in the vertical direction.

3. The exosome detection device according to claim 2, characterized in that: The stirring rod (33) is arranged in an arc shape at one end away from the rotating rod (31), and a slot (6) is provided in the sliding area between the stirring rod (33) and the rotating rod (31); when the stirring rod (33) is placed inside the rotating rod (31), the end of the stirring rod (33) is limited in the slot (6), and the end of the stirring rod (33) is completely matched with the slot (6).

4. The exosome detection device according to claim 1, characterized in that: The driving member is configured as a motor (32), and the motor (32) is vertically arranged on the top of the mixing drum (1). The output end of the motor (32) passes through the mixing drum (1) and is fixedly connected to the top of the rotating rod (31).

5. The exosome detection device according to claim 4, characterized in that: A bottom cover (2) is buckled on the bottom of the mixing drum (1), and a feeding pipe (7) is vertically arranged on the top of the mixing drum.

6. The exosome detection device according to claim 5, characterized in that: The propulsion drive is provided as a second cylinder (52), the second cylinder (52) is vertically arranged on the top of the mixing drum (1), an arc block (53) is fixedly connected to the output end of the second cylinder (52), the arc block (53) is rotatably embedded in the scraping disk (51), and the feeding pipe (7) slides through the scraping disk (51).

7. The exosome detection device according to claim 6, characterized in that: A connecting member (8) is provided between the scraping disc (51) and the rotating rod (31), wherein the connecting member (8) comprises a supporting ring (81), wherein the supporting ring (81) is arranged on the top of the rotating rod (31) and coincides with the axis of the rotating rod (31); and a fixing rod (82) is vertically slidably inserted on the rotating rod (31), wherein the fixing rod (82) is fixedly connected to the bottom of the supporting ring (81); A plurality of telescopic limit members (85) are provided, all of which are arranged at the bottom of the support ring (81); a limit groove (83) matching the limit plate is provided on the scraper plate (51); a first spring (84) is sleeved on the fixing rod (82), one end of which is fixedly connected to the support ring (81) and the other end of which is fixedly connected to the rotating rod (31).

8. The exosome detection device according to claim 7, characterized in that: The telescopic limiting member (85) comprises a supporting tube (851), wherein the supporting tube (851) is vertically fixedly connected to the bottom of the supporting ring (81); and a limiting rod (853) is vertically slidably inserted into the supporting tube (851), wherein the limiting rod (853) and the limiting groove (83) match each other, wherein the limiting rod (853) is connected to the supporting tube (851) via a second spring (852), and when the second spring (852) is in an original state, the limiting rod (853) is inserted into the limiting groove (83).

9. The exosome detection device according to claim 5, characterized in that: A movable plate (9) is slidably arranged inside the bottom cover (2), a through hole (11) is opened at the bottom of the bottom cover (2), and a push rod (10) is also provided, and the push rod (10) is threadedly embedded in the bottom of the movable plate (9).

10. The exosome detection device according to claim 9, characterized in that: The mixing drum (1) is provided with a storage box (12), and a scraper (13) is provided inside the storage box (12).

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