A fully automated bacterial laboratory equipment and a method of using the same

Abstract

The application discloses a kind of full-automatic bacterial experiment equipment and its using method, a full-automatic bacterial experiment equipment includes box, box middle is provided with partition, box is divided into two completely independent operation area by partition, one side is bacterial cryopreservation box, the other side is bacterial operation box;Box top and front are respectively provided with 2 symmetrical sealing door devices, the inboard of the symmetrical sealing door device of box top is equipped with ultraviolet lamp device, box lower is provided with motor and refrigeration heating temperature control system device, box left top is equipped with operation panel, operation panel is equipped with full-automatic bacterial operation software, and operation panel is connected to computer by panel line.This full-automatic bacterial experiment equipment can replace artificial operation bacteria, and the operation steps are simple and efficient, which avoids the problem of bacterial contamination caused by improper operation, improves the safety and accuracy of experimental results.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bacterial experiment instruments, and particularly relates to a full-automatic bacterial experiment equipment and a use method thereof. BACKGROUND

[0002] Bacteria are basic materials for microbiological and life science research. In the medical field, there is a complete set of bacteria for the preparation of diagnostic products, the production of bacterins, the study of microbial pathogenicity, the antibacterial experiment of drugs, and the microbial testing of drugs. Microorganisms have life vitality and are prone to variation or even death during subculture. Therefore, bacterial subculture and preservation are important basic work.

[0003] At present, laboratory bacterial related experiments all need manual operation, which is tedious and time-consuming. Improper operation may cause bacterial contamination, resulting in a series of safety hazards, affecting experimental results, and even harming the operator. Therefore, the operator may be infected with dangerous bacteria. Improper bacterial operation may cause the following hazards:

[0004] 1. Laboratory safety risk: Improper operation in the laboratory may cause bacterial contamination, affecting experimental results and even causing cross contamination in the laboratory, threatening the health of laboratory workers.

[0005] 2. Infection risk: Improper operation may cause workers to directly contact harmful bacteria, increasing the risk of infection. For example, when taking seed jar bacteria, if hand hygiene is not paid attention to or appropriate protective equipment is not worn, the risk of infection with bacteria and toxins will increase.

[0006] 3. Biological hazards: If pathogenic bacteria or toxic bacteria are involved in bacterial operation, improper operation may release these harmful substances into the environment, causing harm to operators and others.

[0007] 4. Occupational health problems: Long-term exposure to incorrect bacterial operation environment may cause various occupational health problems, including but not limited to skin irritation, respiratory diseases, etc.

[0008] 5. Influence on experimental results: Bacterial contamination may cause experimental failure, because miscellaneous bacteria will consume the nutrients of culture medium, change the physicochemical properties of fermentation broth, and affect the yield and quality of products.

[0009] 6. Interference with production process: In industrial production, bacterial contamination may cause production interruption, product quality problems, and even economic losses.

[0010] In summary, the improper handling of bacteria operation mainly reflects the potential threat to human health, the influence on experimental results, product quality problems and economic losses. In order to reduce these hazards, it is imperative to invent a device to replace manual operation of bacteria. SUMMARY

[0011] The purpose of the present application is to solve the above problems in the prior art and provide a full-automatic bacteria experiment device and its use method, which can reduce a series of safety hazards caused by the operation of bacteria. The specific technical scheme is as follows:

[0012] A full-automatic bacteria experiment device, comprising a box body, a partition plate is arranged in the middle of the box body, the box body is divided into two completely independent operation areas by the partition plate, one side is a bacteria cryopreservation box, and the other side is a bacteria operation box; two symmetrical sealing door devices are arranged above and in front of the box body respectively, ultraviolet lamp devices are arranged on the inner sides of the symmetrical sealing door devices above the box body, a motor and a refrigeration and heating temperature control system device are arranged below the box body, an operation panel is arranged at the upper left of the box body, the operation panel is internally provided with full-automatic bacteria operation software, and the operation panel is connected to a computer through a panel line.

[0013] Preferably, a test tube rotating disc a at one side of the bacteria cryopreservation box transmits test tubes to a consumable area a through a test tube guide rail a, a culture dish sampling area a transmits culture dishes to the consumable area a through a culture dish guide rail a, a multifunctional mechanical arm a grabs the test tubes or culture dishes in the consumable area a and is transported to the other side of the bacteria cryopreservation box through a mechanical arm running track a; the test tubes or culture dishes transported to the other side of the bacteria cryopreservation box by the mechanical arm running track a enter a cryopreservation tube bottle loading area, the test tubes or culture dishes in the cryopreservation tube bottle loading area enter a cryopreservation bottle rotating disc, are transported to a laser printing area through a cryopreservation bottle guide rail, and are transported to a low-temperature preservation box through a cryopreservation track disc.

[0014] Preferably, a waste storage box a is further arranged in the bacteria cryopreservation box, and used consumables are collected in the waste storage box a.

[0015] Preferably, a rotating guide rail is arranged in the test tube rotating disc a, and a plurality of suspensions, freeze-dried powders and the like in the test tubes placed in the test tube rotating disc a are shaken evenly through the rotating guide rail; a rotating guide rail is also arranged in the cryopreservation bottle rotating disc, and the cryopreservation bottles are transported to the cryopreservation tube track disc through the rotating guide rail.

[0016] Preferably, a lifting platform a is arranged below the cryopreservation tube track disc, an ultraviolet-visible light area a is arranged on the left side of the device, and a light source is emitted from the left side; the lifting platform a distributes the cryopreservation tubes to different layers in the low-temperature preservation box, and the ultraviolet-visible light area a discriminates or determines the content of a substance according to the absorbance at the characteristic wavelength on the absorption spectrum.

[0017] Preferably, one side of the bacteria operation box inside is the same as the bacteria freezing box, including test tube turntable b, culture dish sample area b, test tube track b, culture dish track b, waste storage box b, consumable area b, multifunctional mechanical arm b, mechanical arm running track b, and the other side is provided with a culture dish tray area, a test tube bottle area, a test tube track tray, a culture dish track tray, a test tube incubator, and a culture dish incubator; the test tube in the test tube bottle area is transported to the test tube incubator through the test tube track tray, and the culture dish in the culture dish tray area is transported to the culture dish incubator through the culture dish track tray.

[0018] Preferably, the test tube track tray is arranged in front of the test tube incubator, the culture dish track tray is arranged in front of the culture dish incubator, the lifting platform b is arranged directly below the test tube track tray and the culture dish track tray, the laser printing area is arranged directly above the culture dish track 2 and the test tube track 2, the ultraviolet-visible light area b is arranged on the left side of the equipment, the light source is emitted from the left side, and the optical imaging system is arranged directly above the culture dish track b; the lifting platform b distributes the frozen tubes to different layers in the incubator, the laser printing area performs information collection work after the strain completes freezing, the ultraviolet-visible light area b discriminates or determines the content of the substance according to the absorbance at the characteristic wavelength on the absorption spectrum, and the optical imaging system is connected to the computer through a data line to perform image analysis.

[0019] Preferably, the multifunctional mechanical arm a and the multifunctional mechanical arm b are each provided with 10 arms, which are respectively a culture dish opening and closing clamp, a sampling needle, a magnetic bead clamping forceps, an inoculation ring, a screw bottle knob, a homogenizing clamp, a test tube opening and closing clamp, a grinding wheel, a sealing device, and a high-pressure spray head.

[0020] A use method of a full-automatic bacteria experiment equipment, which is used for liquid strain magnetic bead freezing and includes the following steps:

[0021] Step 1: First, set the strain freezing program and sample information on the operation panel, and then add the used consumables and reagents;

[0022] Step 2: Place the liquid bacterial suspension test tube on the test tube turntable a, rotate the test tube turntable a to transport the liquid bacterial suspension test tube to the test tube guide rail a, and then transport the liquid bacterial suspension test tube to the consumable area a through the test tube guide rail a; at the same time, analyze the sample concentration by the ultraviolet-visible light area and calculate the freezing strain sampling amount according to the set concentration;

[0023] Step 3: Open the liquid bacterial suspension test tube by the test tube opening and closing clamp on the multifunctional mechanical arm a, first blow the liquid bacterial suspension in the liquid bacterial suspension test tube by the sampling needle on the multifunctional mechanical arm a, then suck the required amount of sample after ensuring that the liquid bacterial suspension is mixed, close the test tube by the test tube opening and closing clamp on the multifunctional mechanical arm a, transport the used sample to the waste storage box a through the test tube guide rail a, and transport the liquid bacterial suspension test tube to the freezing bottle turntable by the multifunctional mechanical arm a on the mechanical arm running track a.

[0024] Step 4: the multi-functional mechanical arm a opens the screw bottle knob to open the cryopreservation tube cover, the multi-functional mechanical arm a samples the needle to add the sample into the cryopreservation tube, the multi-functional mechanical arm a screw bottle knob closes the cryopreservation tube cover, and the multi-functional mechanical arm a homogenization clamp hand homogenizes the cryopreservation tube;

[0025] Step 5: after homogenization, the cryopreservation tube is placed back on the cryopreservation bottle turntable, first, the multi-functional mechanical arm a screw bottle knob opens the cryopreservation tube cover, then the multi-functional mechanical arm a sampling needle sucks out all the liquid in the cryopreservation tube, then the multi-functional mechanical arm a screw bottle knob closes the cryopreservation tube cover, and finally the multi-functional mechanical arm a automatic sealing device seals the cryopreservation tube;

[0026] Step 6: the cryopreservation bottle turntable transports the cryopreserved sample to the cryopreservation bottle guide rail, marks the sample related information through the laser printing area, the cryopreservation bottle guide rail transports the sample to the cryopreservation track disc, and the cryopreservation track disc transports the sample to the low-temperature preservation box through lifting, and thus the program ends.

[0027] Compared with the closest prior art, the technical scheme provided by the present application has the following beneficial effects:

[0028] The full-automatic bacteria experiment equipment can replace manual operation of bacteria, and the full-automatic operation steps are simple and efficient, realizing full automation of bacteria related tests, avoiding problems of bacterial pollution caused by improper operation, improving experimental safety and experimental result accuracy, and saving a large amount of time and labor cost for basic scientific research, clinical diagnosis, disease prevention and control, and drug development. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 It is a whole schematic view of the full-automatic bacteria experiment equipment of the present application;

[0030] Figure 2 It is a schematic view of the inside of the box body of the bacteria cryopreservation box of the present application;

[0031] Figure 3 It is a schematic view of the inside of the box body of the bacteria operation box of the present application;

[0032] Figure 4 It is a schematic view of the multi-functional mechanical arm of the present application;

[0033] Figure 5 It is a schematic view of the lifting platform of the present application;

[0034] Wherein: 1, bacteria freezing box, 2, bacteria operation box, 3, sealing door device, 4 ultraviolet lamp device, 5, operation panel, refrigeration and heating temperature control system device 6, 1-1, test tube turntable a, 1-2, culture dish sample area a, 1-3, test tube guide rail a, 1-4, culture dish guide rail a, 1-5, waste storage box a, 1-6, consumable area a, 1-7, multifunctional mechanical arm a, 1-8, mechanical arm running track a, 1-9, freezing tube bottle area, 1-10, freezing bottle turntable, 1-11, freezing bottle guide rail, 1-12, laser printing area, 1-13, freezing track disc, 1-14, low temperature preservation box, 1-15, lifting platform a, 1-16, ultraviolet visible light area a, 2-1, test tube turntable b, 2-2, culture dish sample area b, 2-3, test tube guide rail b, 2-4, culture dish guide rail b, 2-5, waste storage box b, 2-6, consumable area b, 2-7, multifunctional mechanical arm b, 2-8, mechanical arm running track b, 2-9, culture dish upper disc area, 2-10, test tube bottle area, 2-11, test tube track disc, 2-12, culture dish track disc, 2-13, test tube incubator, 2-14, culture dish incubator, 2-15, track disc track frame, 2-16, lifting platform b, 2-17, laser printing area, 2-18, ultraviolet visible light area b, 2-19, optical imaging system, 2-20 culture dish track 2, 2-21 test tube track 2. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0036] Please refer to Figures 1-5 .

[0037] Embodiment 1

[0038] A full-automatic bacteria experiment equipment includes a box body, a partition plate is arranged in the middle of the box body, the box body is divided into two completely independent operation areas through the partition plate, one side is a bacteria freezing box 1, and the other side is a bacteria operation box 2; two symmetrical sealing door devices 3 are arranged on the upper and front of the box body, respectively, an ultraviolet lamp device 4 is arranged on the inner side of the symmetrical sealing door devices 3 on the upper of the box body, a motor and a refrigeration and heating temperature control system device 6 are arranged on the lower of the box body, an operation panel 5 is arranged on the upper left of the box body, the operation panel 5 is internally provided with full-automatic bacteria operation software, and the operation panel 5 is connected to a computer through a panel line.

[0039] Further, the test tube carousel a1-1 inside the bacterial freezer box 1 transmits the test tubes to the consumable area a1-6 through the test tube guide rail a1-3, the culture dish sample area a1-2 transmits the culture dishes to the consumable area a1-6 through the culture dish guide rail a1-4, and the bacterial freezer box 1 is also provided with a waste storage box a1-5, and the used consumables are collected in the waste storage box a1-5; the multifunctional mechanical arm a1-7 grabs the test tubes or culture dishes in the consumable area a1-6 and is transported to the other side inside the bacterial freezer box 1 through the mechanical arm running rail a1-8; the test tubes or culture dishes transported by the mechanical arm running rail a1-8 to the other side inside the bacterial freezer box 1 enter the cryogenic tube bottle area 1-9, the test tubes or culture dishes in the cryogenic tube bottle area 1-9 enter the cryogenic bottle carousel 1-10, are transported to the laser printing area 1-12 through the cryogenic bottle guide rail 1-11, and are transported to the low-temperature preservation box 1-14 through the cryogenic rail disc 1-13.

[0040] Further, the test tube carousel a1-1 inside the bacterial freezer box 1 transmits the test tubes to the consumable area a1-6 through the test tube guide rail a1-3, the culture dish sample area a1-2 transmits the culture dishes to the consumable area a1-6 through the culture dish guide rail a1-4, and the bacterial freezer box 1 is also provided with a waste storage box a1-5, and the used consumables are collected in the waste storage box a1-5; the multifunctional mechanical arm a1-7 grabs the test tubes or culture dishes in the consumable area a1-6 and is transported to the other side inside the bacterial freezer box 1 through the mechanical arm running rail a1-8; the test tubes or culture dishes transported by the mechanical arm running rail a1-8 to the other side inside the bacterial freezer box 1 enter the cryogenic tube bottle area 1-9, the test tubes or culture dishes in the cryogenic tube bottle area 1-9 enter the cryogenic bottle carousel 1-10, are transported to the laser printing area 1-12 through the cryogenic bottle guide rail 1-11, and are transported to the low-temperature preservation box 1-14 through the cryogenic rail disc 1-13.

[0041] Further, the lifting platform a1-15 distributes the cryogenic tubes to different layers in the low-temperature preservation box, and the ultraviolet-visible light area a1-16 discriminates or determines the content of the substance according to the absorbance at the characteristic wavelength on the absorption spectrum.

[0042] Further, the inside of the bacterial operation box 2 at one side is the same as that of the bacterial freezer box 1, including the test tube carousel b2-1, the culture dish sample area b2-2, the test tube rail b2-3, the culture dish rail b2-4, the waste storage box b2-5, the consumable area b2-6, the multifunctional mechanical arm b2-7, and the mechanical arm running rail b2-8, and the other side is provided with the culture dish carousel area 2-9, the test tube bottle area 2-10, the test tube rail disc 2-11, the culture dish rail disc 2-12, the test tube incubator 2-13, and the culture dish incubator 2-14; the test tubes in the test tube bottle area 2-10 are transported to the test tube incubator 2-13 through the test tube rail disc 2-11, and the culture dishes in the culture dish carousel area 2-9 are transported to the culture dish incubator 2-14 through the culture dish rail disc 2-12.

[0043] Further, the lifting platform b2-16 distributes the cryogenic tubes to different layers in the incubator, the laser printing area 2-17 performs information collection work after the bacteria are completed, the ultraviolet-visible light area b2-18 discriminates or determines the content of the substance according to the absorbance at the characteristic wavelength on the absorption spectrum, and the optical imaging system 2-19 is connected to the computer through a data line to perform image analysis.

[0044] Further, the multifunctional robot arm a1-7 and the multifunctional robot arm b2-7 are each provided with 10 arms, which are respectively a cover opening and closing clamp hand, a sampling needle, a magnetic bead clamping forceps, an inoculation ring, a screw neck bottle knob, a homogenizing clamp hand, a test tube opening and closing clamp hand, a grinding wheel, a sealing device, and a high-pressure spray head.

[0045] Further, the robot arm running track runs through the entire box, and is transported from one side of the box to the other side.

[0046] A use method of a full-automatic bacterial experiment equipment, using the full-automatic bacterial experiment equipment to perform liquid strain magnetic bead cryopreservation, comprising the following steps:

[0047] Step 1: turn on the machine, set the strain cryopreservation program and sample information on the operation panel 5, and add the consumables and reagents used;

[0048] Step 2: place the liquid strain suspension test tube on the test tube turntable a1-1, rotate the test tube turntable a1-1 to transport the liquid strain suspension test tube to the test tube guide rail a1-3, and transport it to the consumable area a1-6 through the test tube guide rail a1-3; at the same time, analyze the sample concentration through the ultraviolet-visible light area 1-16 and calculate the cryopreserved strain sampling amount according to the set concentration;

[0049] Step 3: open the liquid strain suspension test tube through the test tube opening and closing clamp hand on the multifunctional robot arm a1-7, first blow the liquid strain suspension test tube through the sampling needle on the multifunctional robot arm a1-7 to ensure that the liquid is mixed, then suck the required amount of sample, cover the test tube with the test tube opening and closing clamp hand on the multifunctional robot arm a1-7, transport the used sample to the waste storage box a1-5 through the test tube guide rail a1-3, and transport the liquid strain suspension test tube to the top of the cryopreservation bottle turntable 1-10 through the multifunctional robot arm a1-7 on the robot arm running track a1-8;

[0050] Step 4: open the cryopreservation tube cover with the screw neck bottle knob on the multifunctional robot arm a1-7, add the sample into the cryopreservation tube with the sampling needle on the multifunctional robot arm a1-7, close the cryopreservation tube cover with the screw neck bottle knob on the multifunctional robot arm a1-7, and homogenize the cryopreservation tube with the homogenizing clamp hand on the multifunctional robot arm a1-7,

[0051] Step 5: after homogenization, place the cryopreservation tube back on the cryopreservation bottle turntable 1-10, first open the cryopreservation tube cover with the screw neck bottle knob on the multifunctional robot arm a1-7, then completely suck out the liquid in the cryopreservation tube with the sampling needle on the multifunctional robot arm a1-7, then close the cryopreservation tube cover with the screw neck bottle knob on the multifunctional robot arm a1-7, and finally seal the cryopreservation tube with the automatic sealer on the multifunctional robot arm a1-7;

[0052] Step 6: The frozen sample is transported to the frozen bottle guide rail 1-11, the sample information is marked by the laser printing area 1-12, the frozen bottle guide rail 1-11 transports the sample to the frozen track disc 1-13, the frozen track disc 1-13 transports the sample to the low-temperature storage box 1-14 through the lifting platform 1-15, and the procedure ends.

[0053] Example 2

[0054] A method for using a full-automatic bacterial experiment equipment, using the full-automatic bacterial experiment equipment to perform a solid bacterial purification operation process, comprising the following steps:

[0055] Step 1: Turn on the machine, set the solid bacterial purification program and sample information on the computer or operation panel, and add the consumables and reagents used;

[0056] Step 2: Place the sample to be purified on the sample area b 2-2, and transport the sample to the culture dish track b 2-4, and quickly scan and image through the optical imaging system 2-19, and analyze the image through the data line connected to the computer to find the target colony;

[0057] Step 3: The multi-functional mechanical arm b 2-7 opens the lid with the plate opening and closing clamp, collects the target colony with the inoculation ring, and closes the lid with the plate opening and closing clamp, the culture dish track b 2-4 transports the used sample to the waste storage box b 2-5, and the multi-functional mechanical arm b 2-7 runs on the mechanical arm running track b 2-8 to the culture dish track, and the culture dish track continues to transport the medium with the sample information printed by the laser printing area 2-17;

[0058] Step 4: The multi-functional mechanical arm b 2-7 opens the medium cover with the plate cover opening and closing clamp, divides the line on the medium with the inoculation ring used to collect the sample, closes the medium cover with the plate cover opening and closing clamp, and the multi-functional mechanical arm b 2-7 transports the used inoculation ring to the waste storage box b 2-5, the culture dish track transports the medium to the culture dish track disc b 2-12, and the culture dish track disc b 2-12 transports the medium to the culture dish incubator b 2-14 through the lifting platform b 2-16, and the procedure ends.

[0059] Example 3

[0060] A method for using a full-automatic bacterial experiment equipment, using the full-automatic bacterial experiment equipment to perform a microbial limit test operation process, comprising the following steps:

[0061] Step 1: Turn on, set up the microbial limit test program and sample information on the computer or operation panel, add the consumables and reagents used;

[0062] Step 2: Place the sample on the test tube turntable b 2-1, according to the set sample dilution concentration, the test tube opener on the multifunctional manipulator b 2-7 opens the test tube, the sampling needle on the multifunctional manipulator b 2-7 sucks a certain amount of diluent into the test tube from the consumable area b 2-6, the test tube opener on the multifunctional manipulator b 2-7 closes the test tube, and the homogenization clamp on the multifunctional manipulator b 2-7 homogenizes the test tube (the homogenization method can be set, including but not limited to shaking and overturning), and after homogenization, it is placed back on the test tube turntable b 2-1;

[0063] Step 3: The test tube opener on the multifunctional manipulator b 2-7 opens the second test tube, the sampling needle on the multifunctional manipulator b 2-7 sucks a certain amount of diluent into the second test tube from the consumable area b 2-6, the test tube opener on the multifunctional manipulator b 2-7 opens the first test tube, and the sampling needle on the multifunctional manipulator b 2-7 sucks a certain amount of sample into the second test tube from the first test tube;

[0064] Step 4: The test tube opener on the multifunctional manipulator b 2-7 closes the first and second test tubes, the homogenization clamp on the multifunctional manipulator b 2-7 homogenizes the second test tube (the homogenization method can be set, including but not limited to shaking and overturning), and after homogenization, it is placed back on the test tube turntable b 2-1 (if further gradient dilution is required, the same method is performed according to the above steps);

[0065] Step 5: The test tube turntable b 2-1 transports the sample to the test tube track b 2-3, the test tube opener on the multifunctional manipulator b 2-7 opens the first test tube, and the sampling needle on the multifunctional manipulator b 2-7 sucks a certain amount of liquid in the first test tube, while the multifunctional manipulator b 2-7 runs on the manipulator running track b 2-8 to the petri dish track;

[0066] Step 6: The petri dish track continues to transport the culture medium with sample information printed by the laser printing area 2-17, the petri dish cover opener on the multifunctional manipulator b 2-7 opens the culture medium cover with sample information printed, the sampling needle on the multifunctional manipulator b 2-7 sucks the liquid in the first test tube and injects it into the culture medium with sample information printed, and the multifunctional manipulator transports the used sampling needle to the waste storage box b 2-5,

[0067] Step 7: The multi-functional mechanical arm b 2-7 opens the Petri dish cover clamping hand, and the multi-functional mechanical arm b 2-7 homogenizes the medium in the first test tube (the homogenization method can be set, including but not limited to shaking and overturning). After homogenization, the multi-functional mechanical arm b 2-7 puts the medium back on the Petri dish track, the multi-functional mechanical arm b 2-7 opens the second test tube with the test tube opener, the multi-functional mechanical arm b 2-7 sucks a certain amount of liquid in the second test tube with the sampling needle, and the multi-functional mechanical arm b 2-7 runs on the mechanical arm running track b 2-8 to the Petri dish track;

[0068] Step 8: The Petri dish track continues to transport the medium printed with sample information by the laser printing area 2-17, the multi-functional mechanical arm b 2-7 opens the Petri dish cover clamping hand, the multi-functional mechanical arm b 2-7 sucks the liquid in the second test tube into the medium printed with sample information, the multi-functional mechanical arm b 2-7 transports the used sampling needle to the waste storage box b 2-5, the multi-functional mechanical arm b 2-7 closes the Petri dish cover clamping hand, and the multi-functional mechanical arm b 2-7 homogenizes the medium in the second test tube (the homogenization method can be set, including but not limited to shaking and overturning);

[0069] Step 9: The test tube guide rail b 2-3 transports the used test tube to the waste storage box a 1-5, and the Petri dish track puts the medium back on the Petri dish track after homogenization. The Petri dish track transports the medium to the Petri dish track plate 2-12, the Petri dish track plate 2-12 transports the medium to the Petri dish incubator 2-14 through the lifting table a 1-15, and the procedure ends.

[0070] Example 4

[0071] A method for using a full-automatic bacterial experiment device, using the full-automatic bacterial experiment device to perform a drug minimum inhibitory concentration MIC determination process, including the following steps:

[0072] Step 1: Turn on the computer or operation panel, set the drug minimum inhibitory concentration MIC determination program and sample information, and add the consumables and reagents (drugs) used;

[0073] Step 2: Place the bacterial suspension in the test tube carousel b 2-1, which will rotate to transport the bacterial suspension to the test tube rail a 1, analyze the sample concentration by ultraviolet-visible light area b 2-18, and calculate the sampling amount of the bacterial solution according to the set concentration. The test tube opener on the multifunctional manipulator a 1-7 opens test tubes 1, 2, 3 (1, 2, 3 are the first gradient parallel three groups), 4, 5, 6 (4, 5, 6 are the second gradient parallel three groups), 7, 8, 9 (7, 8, 9 are the third gradient parallel three groups) on the test tube track 2, and the sampling needle on the multifunctional manipulator a 1-7 sucks a certain amount of liquid medium into test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9. The sampling needle on the multifunctional manipulator a 1-7 first blows the bacterial solution to ensure that the bacterial solution is mixed evenly before sucking the same amount of bacterial solution into test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9.

[0074] Step 3: The multifunctional manipulator a 1-7 changes the sampling needle to suck the set concentration of drugs into the first gradient test tubes 1, 2, 3, the multifunctional manipulator a 1-7 changes the sampling needle to suck the set concentration of drugs into the second gradient test tubes 4, 5, 6, and the multifunctional manipulator a 1-7 changes the sampling needle to suck the set concentration of drugs into the third gradient test tubes 7, 8, 9.

[0075] Step 4: The test tube opener on the multifunctional manipulator b 2-7 covers the test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9 with lids, and the homogenization clamp hand on the multifunctional manipulator 2-7 homogenizes the test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9 (the homogenization method can be set, including but not limited to shaking, overturning). After homogenization, they are placed back on the test tube track 2, and the automatic sealer on the multifunctional manipulator b 2-7 seals the test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9 (the sealing method includes but is not limited to sealing film, paraffin), and the laser printing area 2-17 marks the relevant information of the sample. The test tube track 2 transports the test tubes 1, 2, 3, 4, 5, 6, 7, 8, 9 to the test tube track disc b 2-11, and the test tube track disc 2-11 transports the medium to the test tube incubator 2-13 through the lifting table b 2-16. Thus, the program runs.

[0076] Example 5

[0077] A method for using a fully automatic bacterial experiment equipment, using the fully automatic bacterial experiment equipment to perform cleaning and disinfection (using disinfectant method as an example) operation process, including the following steps:

[0078] Step 1: Set the cleaning and disinfection program (including but not limited to disinfectant, dry heat sterilization, ultraviolet, fumigation) and sample information on the computer or operation panel, and then add the consumables and reagents used;

[0079] Step 2: The upper high-pressure nozzle of the multifunctional mechanical arm b 2-7 is provided with a conduit directly connected to a disinfectant bottle, and the disinfectant is transported to the high-pressure nozzle through a pressurization mode. The multifunctional mechanical arm b 2-7 sprays the disinfectant to every place in the equipment by running on the mechanical arm track b 2-8. Thus, the program ends.

[0080] The above examples are only used to illustrate the technical solutions of the present application, but not limit it. Although the present application has been described in detail with reference to the above examples, the ordinary skilled in the art can still modify or equivalently replace the specific embodiments of the present application. Any modification or equivalent replacement without departing from the spirit and scope of the present application is within the protection scope of the claims of the application.

Claims

1. A fully automated bacterial laboratory equipment comprising a cabinet, characterized in that: The box is provided with a partition plate in the middle, which divides the box into two completely independent operation areas, one side is a bacteria freezing box (1), and the other side is a bacteria operation box (2); The upper and front of the box are respectively provided with two symmetrical sealing door devices (3), the inside of the symmetrical sealing door device (3) on the upper side of the box is provided with an ultraviolet lamp device (4), the lower side of the box is provided with a motor and a refrigeration and heating temperature control system device, the upper left side of the box is provided with an operation panel (5), the operation panel (5) is provided with a full-automatic bacteria operation software, and the operation panel (5) is connected to a computer through a panel line; The test tube rotating disc a (1-1) on one side of the bacteria freezing box (1) transmits the test tube to the consumable area a (1-6) through the test tube guide rail a (1-3), the culture dish sampling area a (1-2) transmits the culture dish to the consumable area a (1-6) through the culture dish guide rail a (1-4), the multifunctional mechanical arm a (1-7) grabs the test tube or culture dish in the consumable area a (1-6) and is transported to the other side of the bacteria freezing box (1) through the mechanical arm running track a (1-8); The test tube or culture dish transported by the mechanical arm running track a (1-8) on the other side of the bacteria freezing box (1) enters the freezing tube bottle loading area (1-9), the test tube or culture dish in the freezing tube bottle loading area (1-9) enters the freezing bottle rotating disc (1-10), is transported to the laser printing area (1-12) through the freezing bottle guide rail (1-11), and is transported to the low-temperature preservation box (1-14) through the freezing track disc (1-13); The lifting platform a (1-15) is arranged below the freezing tube track disc (1-13), the ultraviolet visible light area a (1-16) is arranged on the left side of the equipment, and the light source is emitted from the left side; The lifting platform a (1-15) distributes the freezing tubes to different layers in the low-temperature preservation box, and the ultraviolet visible light area a (1-16) discriminates or determines the content of the substance according to the absorbance of the characteristic wavelength on the absorption spectrum; The bacteria operation box (2) is the same as the bacteria freezing box (1) on one side, including the test tube rotating disc b (2-1), the culture dish sampling area b (2-2), the test tube track b (2-3), the culture dish track b (2-4), the waste storage box b (2-5), the consumable area b (2-6), the multifunctional mechanical arm b (2-7), the mechanical arm running track b (2-8), and the other side is provided with the culture dish loading area (2-9), the test tube bottle loading area (2-10), the test tube track disc (2-11), the culture dish track disc (2-12), the test tube incubator (2-13), and the culture dish incubator (2-14); The test tube in the test tube bottle loading area (2-10) is transported to the test tube incubator (2-13) through the test tube track disc (2-11), and the culture dish in the culture dish loading area (2-9) is transported to the culture dish incubator (2-14) through the culture dish track disc (2-12).

2. The fully automated bacterial laboratory apparatus according to claim 1, characterized in that The bacteria freezing box (1) is further provided with a waste storage box a (1-5), and the used consumables are collected in the waste storage box a (1-5).

3. The fully automated bacterial laboratory apparatus according to claim 1, wherein The test tube turntable a (1-1) is internally provided with a rotating guide rail, and the suspension liquid, freeze-dried powder and other substances in the plurality of test tubes placed in the test tube turntable a (1-1) are shaken through the rotating guide rail; the cryogenic bottle turntable (1-10) is also internally provided with a rotating guide rail, and the cryogenic bottle is transported to the cryogenic tube guide rail disc (1-13) through the rotating guide rail.

4. The fully automated bacterial laboratory apparatus according to claim 1, wherein The test tube guide rail disc (2-11) is arranged in front of the test tube incubator (2-13), the culture dish guide rail disc (2-12) is arranged in front of the culture dish incubator (2-14), the lifting platform b (2-16) is arranged directly below the test tube guide rail disc (2-11) and the culture dish guide rail disc (2-12), the laser printing area (2-17) is arranged directly above the culture dish guide rail 2 (2-20) and the test tube guide rail 2 (2-21), the ultraviolet-visible light area b (2-18) is arranged on the left side of the equipment, the light source is emitted from the left side, and the optical imaging system (2-19) is arranged directly above the culture dish guide rail b (2-4); the lifting platform b (2-16) distributes the cryogenic tubes to different layers in the incubator, the laser printing area (2-17) performs information collection work on the bacteria after completing the freezing, the ultraviolet-visible light area b (2-18) discriminates or determines the content of the substance according to the absorbance at the characteristic wavelength on the absorption spectrum, and the optical imaging system (2-19) is connected to the computer through a data line to perform image analysis.

5. The fully automated bacterial laboratory apparatus according to claim 4, characterized in that The multifunctional mechanical arm a (1-7) and the multifunctional mechanical arm b (2-7) are each provided with 10 arms, which are respectively a culture dish opening and closing clamp, a sampling needle, a magnetic bead clamping forceps, an inoculation ring, a screw bottle knob, a homogenizing clamp, a test tube opening and closing clamp, a grinding wheel, a sealing device and a high-pressure spray head.

6. The method of using a fully automated bacterial laboratory apparatus according to any one of claims 1 to 5, characterized in that: The full-automatic bacteria experiment equipment is used to perform liquid bacteria magnetic bead freezing, including the following steps: Step 1: first, the bacteria freezing program and sample information are set on the operation panel (5) after starting the machine, and then the consumables and reagents used are added; Step 2: the liquid bacteria suspension test tube is placed on the test tube turntable a (1-1), the test tube turntable a (1-1) is rotated to transport the liquid bacteria suspension test tube to the test tube guide rail a (1-3), and the test tube guide rail a (1-3) is used to transport the liquid bacteria suspension test tube to the consumable area a (1-6); at the same time, the sample concentration is analyzed through the ultraviolet-visible light area (1-16) and the freezing bacteria sampling amount is calculated according to the set concentration; Step 3: the liquid bacteria suspension test tube is opened through the test tube opening and closing clamp on the multifunctional mechanical arm a (1-7), the sampling needle on the multifunctional mechanical arm a (1-7) first blows the liquid bacteria suspension test tube to ensure that the liquid bacteria suspension test tube is mixed, and then the required amount of sample is sucked, the test tube opening and closing clamp on the multifunctional mechanical arm a (1-7) is used to close the test tube, the used sample is transported to the waste storage box a (1-5) through the test tube guide rail a (1-3), and the multifunctional mechanical arm a (1-7) is used to transport the liquid bacteria suspension test tube to the cryogenic bottle turntable (1-10) through the mechanical arm running rail a (1-8). Step 4: Multifunctional robot arm a (1-7) screw bottle knob to open the cryopreservation tube cover, multifunctional robot arm a (1-7) sampling needle to add sample into the cryopreservation tube, multifunctional robot arm a (1-7) screw bottle knob to close the cryopreservation tube cover, multifunctional robot arm a (1-7) homogenization clamp hand homogenizes the cryopreservation tube, Step 5: After homogenization, the cryopreservation tube is put back on the cryopreservation bottle turntable (1-10), first multifunctional robot arm a (1-7) screw bottle knob opens the cryopreservation tube cover, then multifunctional robot arm a (1-7) sampling needle sucks out all the liquid in the cryopreservation tube, then multifunctional robot arm a (1-7) screw bottle knob closes the cryopreservation tube cover, and finally multifunctional robot arm a (1-7) automatic sealer seals the cryopreservation tube; Step 6: The cryopreservation bottle turntable (1-10) transports the cryopreserved sample to the cryopreservation bottle guide rail (1-11), marks the sample related information through the laser printing area (1-12), the cryopreservation bottle guide rail (1-11) transports the sample to the cryopreservation track disc (1-13), and the cryopreservation track disc (1-13) transports the sample to the low-temperature preservation box (1-14) through the lifting platform (1-15), and thus the program ends.

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