Composition and method for simultaneous delivery of sows and application of composition and method

Abstract

The invention provides a composition and a method for sow simultaneous delivery and application of the composition and the method, and particularly discloses a composition and a method for shortening the delivery process and improving the live piglet rate according to a sow simultaneous delivery scheme applying vitamin B6, mifepristone, cloprostenol sodium and carbetocin. According to the composition, aiming at the problem that the sow production efficiency is influenced by the reduction of the live piglet rate caused by the simultaneous delivery of sows, the vitamin B6 and the mifepristone are combined on the basis of the delivery drugs cloprostenol sodium and carbetocin, so that a set of new simultaneous delivery scheme is formed; according to the method, the delivery process of the sows is shortened, the live piglet rate of the sows is increased, the method is a method for improving the same-period delivery effect of the sows in batches, and great value is provided for improving the PSY of the sows and promoting batch management of the sows.

Description

Technical Field

[0001] The present invention mainly relates to the field of animal reproduction regulation, and specifically provides a method for improving the effect of synchronized farrowing in batch production of sows, and particularly relates to a composition, method and application thereof for synchronized farrowing of sows. Background Art

[0002] The sow batch production technology is to divide the sow group according to the production plan according to the size of the sow group, supplement the reserve sows according to the production plan, and use biotechnology to make the reserve sows and multiparous sows in the same batch reach the purpose of synchronous estrus, synchronous mating and synchronous delivery. It is an efficient and controllable management technology system to improve the reproductive performance of the sow group in the pig farm. Among them, synchronous delivery of sows is one of the key links in sow batch production. After the sow starts to give birth, the uterus contracts rhythmically. The longer the piglets stay in the sow's uterus or birth canal, the higher the risk of umbilical cord rupture, and the greater the risk of piglet death. Especially in the late stage of sow delivery, the sow's production force is insufficient, the uterus is weak, and the piglets with broken umbilical cords cannot be delivered in time, resulting in an increased risk of suffocation and death; in addition, even if the sows are bred at the same time, due to individual differences in sows, the start time of delivery of sows in the same batch is inconsistent, and the delivery time span of the entire batch of sows is about 5-7 days, which makes batch management difficult and seriously affects the efficiency of sow batch production. As the number of high-producing sows increases, the problem of increasing numbers of stillbirths and mummies due to long farrowing processes has become more obvious. Therefore, establishing efficient sow synchronous farrowing technology is the key to ensuring batch production, improving efficiency and achieving precise control.

[0003] Existing synchronous delivery technology often uses cloprostenol sodium, cloprostenol sodium combined with carbetocin or oxytocin to achieve synchronous delivery of sows. However, when cloprostenol sodium is used alone, the delivery time span of batch sows is still large; and the combined use of oxytocin, although it can induce concentrated delivery of sows, is prone to prolonging the delivery process and increasing the number of stillbirths; and the combined use of carbetocin, although it can increase the concentration of sow delivery and shorten the delivery process, the number of live piglets and the live piglet rate are reduced. In view of the above reasons, the pig farming industry urgently needs a simple and efficient batch production sow synchronous delivery technology to replace the original sow synchronous delivery plan.

[0004] Vitamin B6 is a water-soluble B vitamin that exists in the body in the form of pyridoxine, pyridoxal, pyridoxamine, pyridoxine phosphate, pyridoxal phosphate, and pyridoxamine phosphate. It participates in key biochemical processes such as amino acid metabolism and neurotransmitter synthesis. It has physiological functions such as anti-inflammatory, antioxidant, neuroregulatory and anti-tumor, and is essential for maintaining the health of the nervous system, skin health and immune function.

[0005] Mifepristone is an organic compound with the chemical formula C 29 H 35NO2 is a new type of anti-progestin with anti-glucocorticoid activity. It is used as an anti-early pregnancy drug. Its affinity for progesterone receptors is 5 times stronger than that of progesterone. It is used to prevent early pregnancy, induce menstruation and stop pregnancy, and induce labor in case of intrauterine fetal death.

[0006] However, to date, there have been no reports or suggestions on the use of vitamin B6 and mifepristone to shorten the labor process and increase the survival rate of multi-birth animals. Summary of the invention

[0007] The present invention has been subjected to a large number of creative experiments, and the effects of the combined use of vitamin B6, mifepristone, cloprostenol sodium and carbetocin on synchronous delivery are compared. The results show that the combined use of vitamin B6, mifepristone, cloprostenol sodium and carbetocin can effectively shorten the process of synchronous delivery of batch sows and improve the live piglet rate, thereby establishing a method for improving synchronous delivery of batch sows, which provides important value for the promotion and application of batch production of sows. Specifically, the present invention includes the following contents.

[0008] The first aspect of the present invention provides a composition for synchronous farrowing of sows, comprising vitamin B6, mifepristone, cloprostenol sodium and carbetocin.

[0009] In certain embodiments, the composition for synchronized farrowing of sows according to the present invention comprises 0.2-20 g of vitamin B6, 50-1000 mg of mifepristone, 0.3-3 mg of cloprostenol sodium and 20-100 μg of carbetocin.

[0010] The second aspect of the present invention provides a method for improving synchronous farrowing in sows, comprising the step of administering the composition of the first aspect to the sow.

[0011] In certain embodiments, the method for improving synchronous farrowing in sows according to the present invention comprises the step of administering vitamin B6, mifepristone, cloprostenol sodium and carbetocin to the sows after synchronous mating on an appropriate gestational day.

[0012] In certain embodiments, according to the method for improving synchronous farrowing of sows according to the present invention, the method comprises the following steps: (1) Sows are bred at the same time, followed by pregnancy tests to confirm pregnancy; (2) Vitamin B6 is administered to sows during gestation days 100-115; (3) Mifepristone was administered to sows at 112-115 days of gestation; (4) Cloprostenol sodium was administered to sows at 113-115 days of gestation; (5) Carbetocin was administered to sows at 113-115 days of gestation.

[0013] In certain embodiments, according to the method for improving synchronous farrowing of sows according to the present invention, the dosage of vitamin B6 is 0.2-20 g / day, and the administration time of vitamin B6 is 100-113 days of gestation, once a day, and the administration method is oral or injection.

[0014] In certain embodiments, according to the method for improving synchronous farrowing in sows of the present invention, the dosage of mifepristone is 50-1000 mg for a single administration, the administration time of mifepristone is 112-115 days of gestation, and the administration method is oral or injection.

[0015] The third aspect of the present invention provides use of the composition or method according to the present invention in batch production of sows.

[0016] In certain embodiments, according to the application of the present invention, the application comprises: shortening the sow's delivery process and increasing the sow's live piglet rate.

[0017] The method of the present invention is a batch sow synchronous delivery scheme based on the combined administration of vitamin B6, mifepristone, cloprostenol sodium and carbetocin, which greatly shortens the sow's delivery process and improves the sow's live piglet rate. The method of the present invention can effectively alleviate the production problem of the sow's live piglet rate decline in sow batch production, and has great value in improving sow PSY (the number of weaned piglets that each sow can provide per year) and promoting sow batch production. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 Flow chart for synchronized farrowing of batches of sows based on combined administration of vitamin B6, mifepristone, cloprostenol sodium and carbetocin. DETAILED DESCRIPTION

[0020] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0021] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that the upper and lower limits of the scope and each intermediate value therebetween are specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0022] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art to which the invention belongs. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0023] Composition for synchronous farrowing of sows One aspect of the present invention provides a composition for improving synchronous farrowing in sows, in particular a composition for improving synchronous farrowing in batch production sows, which comprises or consists of the following components: vitamin B6, mifepristone, cloprostenol sodium and carbetocin.

[0024] In a preferred embodiment, the composition of the present invention comprises 0.2-20 g (preferably 0.2-10 g, also preferably 0.2-9 g, such as 0.2-8 g, 0.2-7 g, 0.2-6 g, 0.2-5 g, 0.2-4 g, 0.2-3 g, 0.2-2 g, 0.3-2 g, 0.4-2 g, more preferably 0.5-2 g, such as 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0 g, or any range within the above content) of vitamin B6; 50-1000 mg (preferably 100-500 mg, also preferably 100-400 mg, such as 100, 150, 200, 250, 300, 350, 400 The cloprostenol sodium and carbetocin can be used in conventional dosages for sow farrowing in the art, and are not particularly limited thereto.

[0025] Methods to improve synchronous farrowing in sows One aspect of the present invention provides a method for improving synchronous farrowing in sows, particularly a method for improving synchronous farrowing in batch production sows, which comprises the step of administering the composition of the present invention to the sows.

[0026] In a preferred embodiment, the method for batch production of vitamin B6 and mifepristone combined with cloprostenol sodium and carbetocin for sows to give birth in the same period is that the sows after mating in the same period are fed vitamin B6 at 100-115 days of gestation, injected with mifepristone at 112-115 days of gestation, injected with cloprostenol sodium at 113-115 days of gestation, and injected with carbetocin at 113-115 days of gestation. In another preferred embodiment, the method for batch production of vitamin B6 and mifepristone combined with cloprostenol sodium and carbetocin for sows to give birth in the same period is that the sows after mating in the same period are fed vitamin B6 at 100-113 days of gestation, injected with mifepristone at 112-115 days of gestation, injected with cloprostenol sodium at 113-115 days of gestation, and injected with carbetocin at 113-115 days of gestation. In another preferred embodiment, the method for batch production of vitamin B6, mifepristone combined with cloprostenol sodium and carbetocin for synchronous delivery of sows is that the sows, after mating at the same time, are fed vitamin B6 once a day from 100 to 113 days of gestation, are injected with mifepristone on 112 days of gestation, with cloprostenol sodium on 113 days of gestation, and with carbetocin on 114 days of gestation.

[0027] In a preferred embodiment, the method for batch production of sows with simultaneous farrowing by combining vitamin B6, mifepristone, cloprostenol sodium and carbetocin comprises the following steps: (1) Sows are bred at the same time, followed by pregnancy tests, pregnancy confirmation, and then transferred to the farrowing house; (2) Vitamin B6 is administered to sows during gestation days 100-115; (3) Mifepristone was administered to sows at 112-115 days of gestation; (4) Cloprostenol sodium was administered to sows at 113-115 days of gestation; (5) Carbetocin was administered to sows at 113-115 days of gestation.

[0028] In another preferred embodiment, the method for batch production of sows with simultaneous farrowing by using vitamin B6, mifepristone, cloprostenol sodium and carbetocin comprises the following steps: (1) Sows are bred at the same time, followed by pregnancy tests, pregnancy confirmation, and then transferred to the farrowing house; (2) Feed vitamin B6 to sows from 100 to 113 days of gestation, once a day; (3) Mifepristone injection at 112 days of gestation in sows; (4) Sows were injected with cloprostenol sodium at 113 days of gestation; (5) Carbetocin was administered to sows at day 114 of gestation.

[0029] In a preferred embodiment of the method for improving synchronous farrowing of sows, the dosage of vitamin B6 is 0.2-20 g / day, preferably 0.2-15 g / day, further preferably 0.2-10 g / day, more preferably 0.5-10 g / day, such as 0.5-5 g / day, 0.5-4 g / day, 0.5-3 g / day, and the most preferred dosage is 0.5-2 g / day, such as 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0 g, or any range within the above dosage. The administration of vitamin B6 is oral or injection, preferably oral.

[0030] In a preferred embodiment of the method for improving synchronous farrowing of sows, the dosage of mifepristone is 50-1000 mg, preferably 100-500 mg, and also preferably 100-400 mg, such as 100, 150, 200, 250, 300, 350, 400 mg, or any range within the above dosages in a single administration. Mifepristone is administered orally or by injection, preferably by injection.

[0031] application In one aspect of the present invention, the use of the composition or the method described in the present invention in batch production of sows is also provided. The present invention provides a method for synchronous farrowing of sows using vitamin B6, mifepristone combined with cloprostenol sodium and carbetocin, which is used to shorten the sow's delivery process and improve the sow's live piglet rate; vitamin B6, mifepristone combined with cloprostenol sodium and carbetocin can alleviate the production problem of decreased live piglet rate of sows in batch production.

[0032] Example The following shows the application of vitamin B6, mifepristone, cloprostenol sodium and carbetocin in the synchronous farrowing of sows in batch production.

[0033] (1) Experimental methods and grouping Large white sows produced in batches were randomly divided into six groups (a)-(f), namely, cloprostenol sodium group + carbetocin group, vitamin B6 + cloprostenol sodium group + carbetocin group, mifepristone + cloprostenol sodium + carbetocin group, vitamin B6 (low dose group) + mifepristone + cloprostenol sodium + carbetocin group, vitamin B6 (medium dose group) + mifepristone + cloprostenol sodium + carbetocin group, vitamin B6 (high dose group) + mifepristone + cloprostenol sodium + carbetocin group. The sows weighed 250-270 kg and were fed a daily diet of 2.5-3 kg. During the experiment, each group of experimental sows were fed regularly according to the feeding and management requirements of the farm, and had free access to water. The daily diet fed could adopt the formula used for sows during pregnancy (for example, the formula recommended in "Sow Batch Production Management Technology") or adjust the components as needed. The nutritional level of the daily diet used in this embodiment is shown in Table 1 below.

[0034] Table 1 Nutritional levels of sow diets during pregnancy The specific groups are as follows: (a) Cloprostenol sodium group + carbetocin group: Sows were injected with 2 ml of cloprostenol sodium injection at 9:00 am on the 113th day of gestation (each sow) at the Houhai point, and 1 ml of carbetocin injection at 9:00 am on the 114th day of gestation (each sow).

[0035] (b) Vitamin B6 + cloprostenol sodium group + carbetocin group: Sows were fed 2 g (each) vitamin B6 per day at 8:00 am on 108-113 days of gestation until 113 days due date. Sows were injected with 2 ml (each) cloprostenol sodium injection at the Houhai point at 9:00 am on 113 days of gestation, and 1 ml (each) carbetocin injection at 9:00 am on 114 days of gestation.

[0036] (c) Mifepristone + cloprostenol sodium + carbetocin group: 400 mg (each) mifepristone was injected into the posterior sea point at 17:00 on the 112th day of gestation, 2 ml (each) cloprostenol sodium injection was injected into the posterior sea point at 9:00 on the 113th day of gestation, and 1 ml (each) carbetocin injection was injected into the posterior sea point at 9:00 on the 114th day of gestation.

[0037] (d) Vitamin B6 (low-dose group) + mifepristone + cloprostenol sodium + carbetocin group: sows were fed 0.1 g (each) vitamin B6 every day at 8:00 am on the 108th to 113th day of gestation until the sows' expected due date of 113 days. Sows were injected with 200 mg (each) mifepristone at 17:00 pm on the 112th day of gestation into the sea point. Sows were injected with 2 ml (each) cloprostenol sodium injection at 9:00 am on the 113th day of gestation. Sows were injected with 1 ml (each) carbetocin injection at 9:00 am on the 114th day of gestation.

[0038] (e) Vitamin B6 (medium dose group) + mifepristone + cloprostenol sodium + carbetocin group: sows were fed 1.0 g (each) vitamin B6 per day at 8:00 am on 108-113 days of gestation until the sows' expected due date of 113 days. Sows were injected with 200 mg (each) mifepristone at 17:00 pm on the 112th day of gestation into the sea point. Sows were injected with 2 ml (each) cloprostenol sodium injection at 9:00 am on the 113th day of gestation. Sows were injected with 1 ml (each) carbetocin injection at 9:00 am on the 114th day of gestation.

[0039] (f) Vitamin B6 (high-dose group) + mifepristone + cloprostenol sodium + carbetocin group: sows were fed 10.0 g (each) vitamin B6 every day at 8:00 am on 108-113 days of gestation until the sows' expected due date of 113 days. Sows were injected with 200 mg (each) mifepristone at 17:00 pm on the 112th day of gestation into the sea point. Sows were injected with 2 ml (each) cloprostenol sodium injection at 9:00 am on the 113th day of gestation. Sows were injected with 1 ml (each) carbetocin injection at 9:00 am on the 114th day of gestation.

[0040] (2) Observe and record sow farrowing Record the date, time, duration, and farrowing conditions of the sows (including the total number of farrowings, number of live piglets, number of stillbirths, and number of mummies, and calculate the average farrowing process and live piglet rate.

[0041] (3) Statistical analysis SPSS 22.0 software (SPSS GmbH, Munich, Germany) was used for one-way ANOVA and chi-square test. The mean farrowing process, litter size, number of live piglets per litter, number of stillbirths per litter, and number of mummies per litter were analyzed by one-way ANOVA and expressed as mean ± standard deviation. The chi-square test was used to analyze the live piglet rate of sows. P < 0.05 was considered significant.

[0042] (4) Experimental results In the present embodiment, compared with the control group (cloprostenol sodium + carbetocin group), vitamin B6 + cloprostenol sodium + carbetocin group, mifepristone + cloprostenol sodium + carbetocin group, can significantly shorten the delivery process, significantly reduce the number of stillbirths, and significantly improve the live pig rate; in the three groups of vitamin B6 + mifepristone + cloprostenol sodium + carbetocin four combined, when the dosage of vitamin B6 reaches the medium / high dose, the sow delivery process is further shortened, the number of live births increases, the number of stillbirths decreases, and the live pig rate is significantly higher than the above three groups. But when the dosage of vitamin B6 is low, even with mifepristone, cloprostenol sodium and carbetocin, compared with vitamin B6 + cloprostenol sodium + carbetocin group, mifepristone + cloprostenol sodium + carbetocin group, its live pig rate has no significant difference (Table 2).

[0043] Table 2 Effects of different synchronous farrowing plans on sow farrowing process and litter size Note: Different lowercase letters on the right shoulder indicate that the indicators in the same row are significantly different among the groups. P<0.05 .

[0044] Industrial value of this invention: Since the first outbreak of African swine fever in August 2018, the traditional continuous production model has been difficult to adapt to the rapid development of the pig farming industry. In addition, the entire live pig market is facing severe challenges. Sow batch production technology has become an important measure for transformation and upgrading. In the future, the era of small-scale pig farming will become a thing of the past. The emergence of large-scale and batch production methods will inevitably bring earth-shaking changes to the pig farming industry. The problem of low live piglet rate caused by synchronous delivery of sows has seriously affected the benefits of pig farms. However, the present invention is a method for improving the effect of synchronous delivery of sows based on the combination of vitamin B6, mifepristone, cloprostenol sodium and carbetocin ( Figure 1 ), which provides important value for the promotion and application of sow batch production. The present invention effectively improves the survival rate of batch sows.

[0045] The batch sow synchronous delivery technology established by the present invention by combining vitamin B6, mifepristone, cloprostenol sodium and carbetocin can reduce the number of stillbirths in batch delivery sows by 0.59 heads, and effectively increase the number of live piglets born per litter by 0.71 heads. According to the annual number of litters farrowed by each sow in China in 2024 of 2.23 and the survival rate of weaned piglets of 95%, the PSY will be increased by 0.71*2.23*95%=1.50 heads. Therefore, the present invention is an effective way to improve the quality and efficiency of pig farming. At the same time, the present invention also has the advantages of simplicity, high efficiency, strong innovation, broad industrial prospects and value.

[0046] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A composition for synchronous farrowing of sows, characterized in that: It includes vitamin B6, mifepristone, cloprostenol sodium, and carbetocin.

2. The composition for synchronous farrowing of sows according to claim 1, characterized in that It includes 0.2-20 g of vitamin B6, 50-1000 mg of mifepristone, 0.3-3 mg of cloprostenol sodium and 20-100 μg of carbetocin.

3. A method for improving synchronous farrowing of sows, characterized in that: The method comprises the step of administering the composition of claim 1 or 2 to a sow.

4. The method for improving synchronous farrowing of sows according to claim 3, characterized in that: The method comprises the steps of administering vitamin B6, mifepristone, cloprostenol sodium and carbetocin to sows after mating at the same time on appropriate gestation days.

5. The method for improving synchronous farrowing of sows according to claim 3, characterized in that: The following steps are involved: (1) Sows are bred at the same time, followed by pregnancy tests to confirm pregnancy; (2) Vitamin B6 is administered to sows during gestation days 100-115; (3) Mifepristone was administered to sows at 112-115 days of gestation; (4) Cloprostenol sodium was administered to sows at 113-115 days of gestation; (5) Carbetocin was administered to sows at 113-115 days of gestation.

6. The method for improving synchronous farrowing of sows according to claim 3, characterized in that: The dosage of vitamin B6 is 0.2-20 g / day. The administration time of vitamin B6 is between 100-113 days of pregnancy, once a day.

7. The method for improving synchronous farrowing of sows according to claim 3, characterized in that: The dosage of mifepristone is 50-1000 mg per single administration, and the administration time of mifepristone is 112-115 days of pregnancy.

8. The method for improving synchronous farrowing of sows according to claim 3, characterized in that: Vitamin B6, mifepristone, cloprostenol sodium, and carbetocin are administered orally or by injection.

9. Use of the composition according to claim 1 or 2, or the method according to any one of claims 3 to 8 in sow batch production.

10. The use according to claim 9, characterized in that: The applications include: shortening the sow's delivery process and increasing the sow's live piglet rate.

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