A magnetic bead-mediated integrated gene amplification device

Abstract

The present invention relates to the field of medical detection technology, and specifically to an integrated device for gene amplification mediated by magnetic beads, comprising an instrument body and a detection component, wherein the detection component is mounted on the instrument body. The detection component comprises a detection tube and a test tube. The detection tube comprises a first chamber and a second chamber arranged in sequence in a first direction, the first chamber comprises a first side wall and a second side wall, and a flattening member is arranged on the first side wall. An integrated device for gene amplification mediated by magnetic beads of the present invention uses a complete machine to make the sample undergo lysis, cleaning and amplification steps in sequence, blows air into the first chamber or inhales air from the first chamber through the second chamber, and enables a driving member to drive the flattening member to move along the second direction toward or away from the second side wall, thereby improving the reaction efficiency and reaction effect of the integrated device for gene amplification mediated by magnetic beads as a whole.

Description

Technical Field

[0001] The present invention relates to the field of medical detection technology, and in particular to a magnetic bead-mediated gene amplification integrated device. Background Art

[0002] Genetic testing is the use of amplified gene fragments to perform various molecular biological tests to diagnose diseases, predict disease risks, or conduct personalized treatments. In the process of gene amplification, magnetic beads are usually used to capture and purify DNA molecules, which can be amplified by specific primers, such as polymerase chain reaction (PCR), thereby greatly improving the analysis and detection capabilities of DNA molecules.

[0003] The samples used for gene amplification are biological tissue cells, which need to be lysed, washed, amplified, and other steps before optical detection is used to detect genes. In the existing technology, when performing lysis, washing, and amplification operations, samples usually need to be processed by multiple devices in different steps, which makes the detection more troublesome. In addition, during the adsorption of magnetic beads, due to the high dispersion of magnetic beads, the adsorption efficiency is low and the adsorption cannot be achieved. Moreover, the magnetic beads after adsorption will also gather together, which is not conducive to the cleaning operation and affects the quality of cleaning. Summary of the invention

[0004] The present invention provides an integrated device for magnetic bead-mediated gene amplification. By setting a detection component, a whole machine is used to make a sample undergo lysis, cleaning and amplification steps in sequence, so as to solve the problem that in the prior art, when performing lysis, cleaning and amplification operations, the sample usually needs to be processed by multiple devices in different steps respectively, and the detection is relatively troublesome. In the process of adsorbing magnetic beads, due to the high dispersion of the magnetic beads, the adsorption efficiency is low and the adsorption cannot be achieved during adsorption. In addition, the magnetic beads after adsorption will also gather together, which is not conducive to the cleaning operation and affects the cleaning quality.

[0005] The present invention provides an integrated device for gene amplification mediated by magnetic beads, which adopts the following technical scheme: an integrated device for gene amplification mediated by magnetic beads, comprising an instrument body and a detection component, wherein the detection component is mounted on the instrument body; the detection component comprises a detection tube and a test tube; the detection tube and the test tube are sequentially arranged and connected in a first direction, wherein the first direction is a vertical direction; the detection tube comprises a first chamber and a second chamber sequentially arranged in the first direction, wherein the second chamber is located on a side of the first chamber close to the test tube in the first direction; in an initial state, the air pressure in the first chamber and the second chamber is balanced, and the second chamber can blow air into the first chamber Or air is sucked from the first chamber; the first chamber includes a first side wall and a second side wall, which are arranged in sequence around the first direction and are sealed and connected; a plurality of magnetic beads are placed in the first chamber, and the plurality of magnetic beads can be adsorbed onto the second side wall, a flattening member is arranged on the first side wall, the flattening member can move along the second direction toward the side close to the second side wall and flatten the plurality of magnetic beads, the second direction is perpendicular to the first direction, and the second direction is horizontal; an overflow port is opened on the second side wall; a driving member is installed on the first side wall, and the driving member can drive the flattening member to move along the second direction toward or away from the side of the second side wall.

[0006] Furthermore, a magnet is disposed outside the second side wall, and the magnet can move toward or away from the second side wall to be adsorbed on or detached from the plurality of magnetic beads.

[0007] Furthermore, the flattening part includes a mounting frame, a support plate and a flattening membrane; the mounting frame is installed in the first chamber, the flattening membrane is elastic and installed on the mounting frame, the shape of the flattening membrane in the initial state is adapted to the shape of the first side wall, the support plate is arranged on the flattening membrane, and the flattening membrane and the support plate can move along the second direction toward or away from the second side wall.

[0008] Furthermore, an installation groove is provided on the flattening membrane, and an installation frame is fixedly connected in the installation groove. The support plate is fixedly connected to the installation frame, and a boss is defined between the support plate and the installation frame. When the flattening membrane moves along the second direction toward the side close to the second side wall, the installation frame can abut against the second side wall, and then an overflow gap is formed between the support plate and the second side wall through the boss; the first chamber includes a barrel section and a cone section arranged in sequence in the first direction, the barrel section is located on the side of the cone section away from the second chamber in the first direction, and the large end of the cone section is connected to the barrel section; a plurality of support rods are evenly distributed around the first direction on the side of the flattening membrane close to the cone section in the first direction, and the flattening membrane moves along the second direction toward the side close to the second side wall, the support rods can abut against the second side wall, and a flow gap allowing liquid to pass can be defined between two adjacent support rods.

[0009] Furthermore, the mounting frame includes two sealing plates and two sealing rings, the two sealing plates are evenly distributed around the first direction in the first chamber, and the two ends of the sealing plates along the first direction are respectively fixed to the sealing rings; the two ends of the flattening membrane along the first direction are respectively installed on the sealing plates; the two ends of the flattening membrane along the first direction are respectively installed on the sealing rings; the two sealing plates are clamped between the first side wall and the second side wall.

[0010] Furthermore, the first side wall and the second side wall are connected by a plurality of clamps.

[0011] Furthermore, a driving cavity is defined between the flattening membrane and the support plate and the second side wall, and the driving member is a first air pump, which can supply air into the driving cavity or draw air from the driving cavity, so that the flattening membrane and the support plate can move along the second direction toward or away from the second side wall.

[0012] Furthermore, an inlet is provided on the second side wall, and the inlet is located on a side of the overflow port close to the second chamber in the first direction.

[0013] Furthermore, an air inlet pipe is provided on the second chamber, the air inlet pipe is communicated with the second chamber, the air inlet pipe is externally connected to a second air pump, and the second air pump can supply air into the second chamber or draw air from the second chamber.

[0014] Furthermore, a balancing block is provided at the connection between the second chamber and the first chamber, and two balancing holes are provided on the balancing block. The two balancing holes are symmetrically arranged about the central axis of the balancing block in the second direction. The balancing holes are conical, and the small ends of the two balancing holes are arranged face to face and connected in the first direction.

[0015] The beneficial effects of the present invention are as follows: a magnetic bead-mediated gene amplification integrated device of the present invention is provided with a detection component, and a whole machine is used to make the sample successively undergo the steps of lysis, cleaning and amplification, and in the process of lysis, cleaning and amplification, air can be blown into the first chamber through the second chamber when necessary, and the driving member can be started to drive the flattening member to move in the second direction close to or away from the second side wall. During lysis, after the flattening member is used to flatten a plurality of magnetic beads, when the magnetic beads are adsorbed, the magnetic beads can be adsorbed quickly, so as to avoid the magnetic beads being too dispersed in the lysis solution and being unable to be effectively adsorbed. During cleaning, the flattening member is used to flatten a plurality of magnetic beads, so as to improve the cleaning effect of the cleaning solution on the residual lysis solution on the plurality of magnetic beads, and avoid directly adsorbing the magnetic beads to make the magnetic beads aggregate. That is, by blowing air into the first chamber or inhaling air from the first chamber through the second chamber, and enabling the driving member to drive the flattening member to move in the second direction close to or away from the second side wall, the reaction efficiency and reaction effect of the magnetic bead-mediated gene amplification integrated device are improved as a whole. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 A schematic diagram of the overall structure of an embodiment of a magnetic bead-mediated gene amplification integrated device of the present invention;

[0018] Figure 2 A schematic diagram of a detection component of an embodiment of a magnetic bead-mediated gene amplification integrated device of the present invention;

[0019] Figure 3 A top view of a detection component of an embodiment of a magnetic bead-mediated gene amplification integrated device of the present invention;

[0020] Figure 4 for Figure 3 Cross-sectional view along the middle line BB;

[0021] Figure 5 The present invention is a schematic structural diagram of a flattened component of a magnetic bead-mediated gene amplification integrated device.

[0022] In the figure: 100, instrument body; 200, detection component; 300, detection tube; 310, first chamber; 311, first side wall; 312, second side wall; 313, overflow port; 314, inlet; 315, air inlet; 320, second chamber; 321, air inlet pipe; 322, balance block; 330, upper cover; 340, clamp; 400, test tube; 500, flattening piece; 510, mounting frame; 511, sealing plate; 512, sealing ring; 520, support plate; 521, plug; 530, flattening membrane; 531, mounting frame. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] An embodiment of a magnetic bead-mediated gene amplification integrated device of the present invention is as follows Figures 1 to 5 shown.

[0025] A magnetic bead-mediated gene amplification integrated device includes an instrument body 100 and a detection component 200, wherein the detection component 200 is mounted on the instrument body 100. The detection component 200 includes a detection tube 300 and a test tube 400. The detection tube 300 and the test tube 400 are sequentially arranged and connected in a first direction, and the first direction is a vertical direction. The detection tube 300 includes a first chamber 310 and a second chamber 320 sequentially arranged in the first direction, and the second chamber 320 is located on a side of the first chamber 310 close to the test tube 400 in the first direction. In the initial state, the air pressure in the first chamber 310 and the second chamber 320 is balanced, and the second chamber 320 can blow air into the first chamber 310 or inhale air from the first chamber 310.

[0026] The first chamber 310 includes a first side wall 311 and a second side wall 312, which are sequentially arranged around a first direction and are sealed and connected. A plurality of magnetic beads are placed in the first chamber 310, and the plurality of magnetic beads can be adsorbed onto the second side wall 312. A flattening member 500 is arranged on the first side wall 311, and the flattening member 500 can move along the second direction toward the side close to the second side wall 312 and flatten the plurality of magnetic beads. The second direction is perpendicular to the first direction, and the second direction is a horizontal direction. An overflow port 313 is provided on the second side wall 312. A driving member is installed on the first side wall 311, and the driving member can drive the flattening member 500 to move along the second direction toward or away from the side of the second side wall 312.

[0027] Specifically, a magnet is disposed outside the second side wall 312, and the magnet can move toward or away from the second side wall 312 to adsorb or detach from the plurality of magnetic beads. An upper cover 330 is rotatably connected to the first chamber 310. When in use, the upper cover 330 is opened by rotating, and then the sample is added into the first chamber 310.

[0028] The integrated device for magnetic bead-mediated gene amplification includes a lysis step, a cleaning step, and an amplification step; Lysis step: add the sample and the lysis solution into the first chamber 310 (because the air pressure in the first chamber 310 and the second chamber 320 is balanced in the initial state, the sample will remain stable in the first chamber 310 and will not fall into the second chamber 320). Then the second chamber 320 blows air into the first chamber 310 for 5 minutes, and then a plurality of magnetic beads are added into the first chamber 310, and air is continuously blown into the first chamber 310. After 5 minutes, the lysed sample will be carried by the plurality of magnetic beads. After that, the second chamber 320 stops blowing air into the first chamber 310, and the driving member drives the flattening member 500 to move along the second direction toward the side close to the second side wall 312. During the movement, the space between the flattening member 500 and the second side wall 312 will be gradually compressed, so that the plurality of magnetic beads are brought closer to the second side wall 312 in the first chamber 310, until the flattening member 500 can flatten the plurality of magnetic beads on the second side wall 312, and then the plurality of magnetic beads are adsorbed by the external magnet, and air is blown into the first chamber 310 through the second chamber 320 again, so that the lysate is discharged from the overflow port 313. Then, the flattening member 500 is driven by the driving member to move along the second direction away from the second side wall 312, and then the magnet is removed. After lysis, the lysed sample will be carried by the plurality of magnetic beads, and the lysate will be discharged from the overflow port 313.

[0029] Cleaning step: Add the cleaning solution into the first chamber 310, and use the second chamber 320 to blow air into the first chamber 310. After 30 seconds, the second chamber 320 stops blowing air into the first chamber 310, and then the driving member drives the flattening member 500 to move along the second direction toward the side close to the second side wall 312 again, until the flattening member 500 can flatten the multiple magnetic beads on the second side wall 312, and then use the external magnet to adsorb the multiple magnetic beads, and blow air into the first chamber 310 again through the second chamber 320, so that the lysate remaining on the lysed sample will be discharged from the overflow port 313 with the cleaning solution. Then, the driving member drives the flattening member 500 to move along the second direction away from the second side wall 312, and removes the magnet. Repeat several times, and after the last operation, use the second chamber 320 to blow air into the first chamber 310 to dry the multiple magnetic beads for 5-10 minutes. After washing, the lysate remaining on the magnetic beads will be discharged from the overflow port 313 together with the washing solution.

[0030] Amplification step: Add the reaction solution to the first chamber 310, and use the second chamber 320 to blow air into the first chamber 310, and let it stand for 2 minutes, then the second chamber 320 stops blowing air into the first chamber 310, and then uses an external magnet to adsorb multiple magnetic beads, and then the second chamber 320 evacuates air from the first chamber 310, so that the reaction solution in the first chamber 310 can enter the second chamber 320 from the first chamber 310, and fall from the second chamber 320 into the test tube 400. After amplification, the amplified sample will be separated from the multiple magnetic beads and enter the test tube 400, and then the gene can be detected by optical detection.

[0031] This embodiment sets up the detection component 200, uses a whole machine to make the sample go through the steps of lysis, cleaning and amplification in sequence, and in the process of lysis, cleaning and amplification, air can be blown into the first chamber 310 through the second chamber 320 when necessary, so that during lysis, the airflow can be used to slightly stir between the sample and the lysis solution, during cleaning, the airflow can be used to slightly stir between the cleaning solution and multiple magnetic beads, and during amplification, the airflow can be used to slightly stir between the reaction solution and multiple magnetic beads, thereby improving the overall reaction effect and reaction efficiency. And in the process of discharging the lysis solution and the cleaning solution, air can be blown into the first chamber 310 through the second chamber 320, so that the lysis solution after lysis and the cleaning solution after cleaning can be blown out, thereby realizing the liquid discharge operation.

[0032] Moreover, during the lysis and cleaning process, the driving member can be started when necessary to drive the flattening member 500 to move in the second direction toward or away from the second side wall 312, and then the magnetic beads can be adsorbed by the magnet, so that during lysis, after the flattening member 500 is used to flatten the multiple magnetic beads, the magnetic beads can be quickly adsorbed when the magnet adsorbs the magnetic beads, thereby preventing the magnetic beads from being too dispersed in the lysis solution and being unable to be effectively adsorbed. During cleaning, the flattening member 500 is used to flatten the multiple magnetic beads, which can improve the cleaning effect of the cleaning solution on the lysis solution remaining on the multiple magnetic beads, and avoid directly adsorbing the magnetic beads to cause the magnetic beads to agglomerate. That is, by setting the flattening member 500, the adsorption speed of the magnet on the magnetic beads when the magnetic bead-mediated gene amplification integrated device is used, and the cleaning effect of the cleaning solution on the multiple magnetic beads can be improved.

[0033] After the liquid is drained, the second chamber 320 can be used to continue blowing air into the first chamber 310 to dry the multiple magnetic beads. During amplification, the gas in the first chamber 310 is extracted through the second chamber 320 to separate the reaction liquid from the multiple magnetic beads. That is, by setting the second chamber 320 to blow air into the first chamber 310 or to inhale air from the first chamber 310, and by enabling the driving member to drive the flattening member 500 to move along the second direction toward or away from the second side wall 312, the reaction efficiency and reaction effect of the integrated device for gene amplification mediated by magnetic beads are improved as a whole.

[0034] In this embodiment, the flattening member 500 includes a mounting frame 510, a support plate 520, and a flattening film 530. The mounting frame 510 is mounted in the first chamber 310, the flattening film 530 is elastic and mounted on the mounting frame 510, the shape of the flattening film 530 in the initial state is adapted to the shape of the first side wall 311, the support plate 520 is disposed on the flattening film 530, and the flattening film 530 and the support plate 520 can move toward or away from the second side wall 312 along the second direction.

[0035] Specifically, the mounting frame 510 includes two sealing plates 511 and two sealing rings 512. The two sealing plates 511 are evenly distributed in the first chamber 310 around the first direction, and the two ends of the sealing plates 511 along the first direction are respectively fixed to the sealing rings 512. One of the sealing rings 512 is annular, and the other sealing ring 512 is cylindrical and penetrates from top to bottom. The flattening film 530 is respectively installed on the sealing plates 511 around the two ends in the first direction. The flattening film 530 is respectively installed on the sealing rings 512 at the two ends in the first direction. The two sealing plates 511 are sandwiched between the first side wall 311 and the second side wall 312.

[0036] The first side wall 311 and the second side wall 312 are connected by a plurality of clamps 340, and two clamps 340 are respectively disposed at two ends of the first side wall 311 and the second side wall 312 along the first direction. The first side wall 311, the second side wall 312 and the sealing plate 511 are fixed and sealed by the plurality of clamps 340.

[0037] An inlet 314 is formed on the second side wall 312 , and the inlet 314 is located on a side of the overflow port 313 close to the second chamber 320 in the first direction. Lysis solution, magnetic beads, reaction solution, and cleaning solution all enter through the inlet 314 .

[0038] The support plate 520 is provided with a plug 521 , and when the support plate 520 moves along the second direction toward the second side wall 312 , the plug 521 can block the inlet 314 , so that the lysate or the cleaning solution can only be discharged from the overflow port 313 .

[0039] Furthermore, a mounting groove is provided on the flattening film 530, and a mounting frame 531 is fixedly connected in the mounting groove. The side of the mounting frame 531 facing the second chamber 320 along the first direction is an open structure. The support plate 520 is fixedly connected to the mounting frame 531, and a boss is defined between the support plate 520 and the mounting frame 531. The support plate 520 is an arc-shaped plate, and the support plate 520 has a convex surface and a concave surface, and the concave surface is arranged toward the second side wall 312. The flattening film 530 moves along the second direction toward the side close to the second side wall 312, and the mounting frame 531 can abut against the second side wall 312, thereby forming an overflow gap between the support plate 520 and the second side wall 312 through the boss.

[0040] The first chamber 310 includes a barrel section and a cone section arranged in sequence in the first direction, the barrel section is located on the side of the cone section away from the second chamber 320 in the first direction, and the large end of the cone section is connected to the barrel section. A plurality of support rods are evenly distributed around the first direction on the side of the flattening membrane 530 close to the cone section in the first direction, and the flattening membrane 530 moves along the second direction to the side close to the second side wall 312, the support rods can abut against the second side wall 312, and a flow gap allowing liquid to pass can be defined between two adjacent support rods.

[0041] The end of the overflow port 313 away from the second chamber 320 along the first direction is called the upper end, and the end of the installation frame 531 away from the second chamber 320 along the first direction is called the head end. The head end and the upper end are arranged adjacent to each other in the first direction. Then, when the head end of the installation frame 531 abuts against the second side wall 312, when air is blown from the second chamber 320 to the first chamber 310 to discharge the liquid (lysis solution, cleaning solution), the liquid can be restricted from flowing at the head end of the installation frame 531 and discharged from the overflow port 313.

[0042] In this embodiment, a mounting frame 510, a support plate 520 and a flattening membrane 530 are arranged. When in use, when the flattening membrane 530 moves along the second direction toward the second side wall 312 until the support rod abuts against the second side wall 312 and the mounting frame 531 abuts against the second side wall 312, the liquid will be discharged from the overflow port 313 after passing through the flow gap and the overflow gap.

[0043] In this embodiment, a driving cavity is defined between the flattening membrane 530 and the support plate 520 and the second side wall 312, an air inlet 315 is opened on the first side wall 311, and the driving member is a first air pump. The first air pump can supply air into the driving cavity or draw air from the driving cavity, so that the flattening membrane 530 and the support plate 520 can move along the second direction toward or away from the second side wall 312.

[0044] In this embodiment, a driving chamber and a first air pump are provided. When in use, the first air pump is used to provide gas to the driving chamber to apply pressure to the flattening membrane 530 and the support plate 520. The flattening membrane 530 will be deformed under the action of the air pressure, and move along the second direction toward the second side wall 312 together with the support plate 520 and the mounting frame 531. During the movement, the space between the flattening member 500 and the second side wall 312 will be gradually compressed, so that the plurality of magnetic beads will be moved closer to the second side wall 312 in the first chamber 310.

[0045] When the mounting frame 531 is in contact with the second side wall 312, the air pressure continues to increase, which will further reduce the gap between the flattening membrane 530 and the second side wall 312. During this process, the lysis solution, cleaning solution or reaction solution will be evenly spread out. At this time, the magnetic beads will also be confined in the space between the second side wall 312 and the flattening membrane 530, and will be flattened by the joint action of the two.

[0046] In this embodiment, an air inlet pipe 321 is provided on the second chamber 320 , the air inlet pipe 321 is connected to the second chamber 320 , and the air inlet pipe 321 is externally connected to a second air pump, which can supply air into the second chamber 320 or extract air from the second chamber 320 .

[0047] In this embodiment, a balancing block 322 is provided at the connection between the second chamber 320 and the first chamber 310. Two balancing holes are provided on the balancing block 322. The two balancing holes are symmetrically arranged about the central axis of the balancing block 322 in the second direction. The balancing holes are conical, and the small ends of the two balancing holes are arranged face to face in the first direction and are connected. By limiting the size of the small ends of the balancing holes, the air pressure inside the first chamber 310 and the second chamber 320 is balanced in the initial state. After the sample enters the first chamber 310, the sample will remain stable in the first chamber 310 and will not fall into the second chamber 320.

[0048] An electrical control box, a lysis liquid tank, a cleaning liquid tank and a reaction liquid tank are arranged inside the instrument body 100. The lysis liquid tank is filled with lysis liquid, the cleaning liquid tank is filled with cleaning liquid, and the reaction liquid tank is filled with reaction liquid. The lysis liquid tank, the cleaning liquid tank and the reaction liquid tank are all connected to the inlet 314 through a hose. Liquid outlet pumps are arranged inside the lysis liquid tank, the cleaning liquid tank and the reaction liquid tank. The electrical control box is electrically connected to the first air pump, the second air pump and the liquid outlet pumps of the lysis liquid tank, the cleaning liquid tank and the reaction liquid tank, and the operations required for lysis, cleaning and amplification are realized through electrical control.

[0049] In combination with the above embodiments, the specific working process is as follows:

[0050] When in use, the lysis operation is first performed: the upper cover 330 is opened to add the sample into the first chamber 310 (because the air pressure in the first chamber 310 and the second chamber 320 is balanced in the initial state, the sample will remain stable in the first chamber 310 and will not fall into the second chamber 320). Then the lysis solution is sent into the first chamber 310 from the inlet 314.

[0051] The second air pump is started to blow air into the second chamber 320. The gas will blow from the second chamber 320 to the first chamber 310. The air flow is used to slightly stir the sample and the lysate to improve the reaction effect. After 5 minutes, multiple magnetic beads are added to the first chamber 310, and air is continuously blown into the first chamber 310. After 5 minutes, the lysed sample will be carried by the multiple magnetic beads. Then the second air pump is turned off to stop blowing air into the first chamber 310.

[0052] The first air pump is started to provide gas to the driving chamber to apply pressure to the flattening film 530 and the support plate 520. The flattening film 530 will be deformed under the action of the air pressure, and move along the second direction to the side close to the second side wall 312 together with the support plate 520 and the mounting frame 531. During the movement, the space between the flattening member 500 and the second side wall 312 will be gradually compressed, so that the plurality of magnetic beads will move closer to the second side wall 312 in the first chamber 310. When the mounting frame 531 abuts against the second side wall 312, the air pressure continues to increase, which will further reduce the gap between the flattening film 530 and the second side wall 312. In this process, the lysate will be evenly spread, and the magnetic beads will also be confined in the space between the second side wall 312 and the flattening film 530, and will be flattened under the joint action of the two. Afterwards, the plurality of magnetic beads are adsorbed by an external magnet, and when the magnet adsorbs the flattened magnetic beads, the magnetic beads can be adsorbed quickly to prevent the magnetic beads from being too dispersed in the lysate and unable to be effectively adsorbed. The second air pump is started again to blow air into the first chamber 310 through the second chamber 320, so that the lysate is discharged from the overflow port 313.

[0053] Then, the first air pump is started to draw air from the driving chamber to apply pressure to the flattening film 530 and the support plate 520. Under the action of the air pressure, the flattening film 530, the support plate 520, and the mounting frame 531 move along the second direction to the side away from the second side wall 312 to reset, and then the magnet is removed. After lysis, the lysed sample will be carried by the multiple magnetic beads, and the lysate will be discharged from the overflow port 313.

[0054] Then, a cleaning operation is performed, and a cleaning solution is added into the first chamber 310 from the inlet 314, and then the second air pump is started to blow air into the second chamber 320, and the gas will be blown from the second chamber 320 to the first chamber 310, and the air flow is used to slightly stir between the cleaning solution and the plurality of magnetic beads to improve the reaction effect. After 30S, the second air pump stops, and then the first air pump is started, and the driving chamber provides gas, so that the flattening film 530 moves along the second direction to the side close to the second side wall 312 together with the support plate 520 under the action of air pressure, and in the repeated cracking operation, the operation of flattening the magnetic beads by using the flattening film 530 and the support plate 520 is repeated because the operation modes of the two are exactly the same. Afterwards, multiple magnetic beads are adsorbed by an external magnet, and the second air pump is started again to blow air into the first chamber 310 through the second chamber 320, so that the residual lysate on the sample after being cracked will be discharged from the overflow port 313 with the cleaning solution. Then start the first air pump to draw air from the drive chamber to apply pressure to the flattening membrane 530 and the support plate 520. Under the action of the air pressure, the flattening membrane 530 will move and reset along the second direction away from the second side wall 312 together with the support plate 520, and then remove the magnet.

[0055] Repeat several times (2 times), and after the last operation, start the second air pump to blow air into the second chamber 320 to dry the multiple magnetic beads for 5-10 minutes. After washing, the lysate remaining on the multiple magnetic beads will be discharged from the overflow port 313 together with the washing solution.

[0056] Finally, the amplification operation is performed: the reaction solution is added to the first chamber 310, and then the second air pump is started to blow air into the second chamber 320. The gas will blow from the second chamber 320 to the first chamber 310, and the air flow is used to slightly stir between the magnetic beads and the reaction solution to improve the reaction effect. After that, the multiple magnetic beads are adsorbed by an external magnet, and then the first air pump is started to pump air from the second chamber 320. The second chamber 320 slowly pumps air from the first chamber 310, so that the reaction solution in the first chamber 310 can enter the second chamber 320 from the first chamber 310, and fall from the second chamber 320 into the test tube 400. After amplification, the amplified sample will be separated from the multiple magnetic beads and enter the test tube 400, and then the gene can be detected by optical detection.

[0057] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A magnetic bead-mediated gene amplification integrated device, characterized in that: The invention comprises an instrument body and a detection component, wherein the detection component is installed on the instrument body; the detection component comprises a detection tube and a test tube; the detection tube and the test tube are sequentially arranged and connected in a first direction, wherein the first direction is a vertical direction; the detection tube comprises a first chamber and a second chamber sequentially arranged in the first direction, wherein the second chamber is located on a side of the first chamber close to the test tube in the first direction; in an initial state, the air pressure in the first chamber and the second chamber is balanced, and the second chamber can blow air into the first chamber or inhale air from the first chamber; the first chamber comprises a first side wall and a second side wall, wherein the first side wall and The second side walls are arranged in sequence around the first direction and are sealed and connected; a plurality of magnetic beads are placed in the first chamber, and the plurality of magnetic beads can be adsorbed onto the second side wall; a flattening member is arranged on the first side wall, and the flattening member can move along the second direction toward the side close to the second side wall and flatten the plurality of magnetic beads, the second direction is perpendicular to the first direction, and the second direction is a horizontal direction; an overflow port is opened on the second side wall; a driving member is installed on the first side wall, and the driving member can drive the flattening member to move along the second direction toward or away from the side of the second side wall; the flattening member includes a mounting frame, a support plate and a flattening film; The mounting frame is installed in the first chamber, the flattening membrane is elastic and mounted on the mounting frame, the shape of the flattening membrane in the initial state is adapted to the shape of the first side wall, the support plate is arranged on the flattening membrane, and the flattening membrane and the support plate can move along the second direction toward or away from the second side wall.

2. The integrated device for magnetic bead-mediated gene amplification according to claim 1, characterized in that: A magnet is arranged outside the second side wall, and the magnet can move toward or away from the second side wall to be adsorbed on or detached from the plurality of magnetic beads.

3. The integrated device for magnetic bead-mediated gene amplification according to claim 1, characterized in that: The flattening membrane is provided with an installation groove, in which an installation frame is fixedly connected, the support plate is fixedly connected to the installation frame, and a boss is defined between the support plate and the installation frame, and when the flattening membrane moves along the second direction toward the side close to the second side wall, the installation frame can abut against the second side wall, and then an overflow gap is formed between the support plate and the second side wall through the boss; the first chamber includes a barrel section and a cone section arranged in sequence in the first direction, the barrel section is located on the side of the cone section away from the second chamber in the first direction, and the large end of the cone section is connected to the barrel section; a plurality of support rods are evenly distributed around the first direction on the side of the flattening membrane close to the cone section in the first direction, and the flattening membrane moves along the second direction toward the side close to the second side wall, the support rods can abut against the second side wall, and a flow gap allowing liquid to pass can be defined between two adjacent support rods.

4. The integrated device for magnetic bead-mediated gene amplification according to claim 1, characterized in that: The mounting frame includes two sealing plates and two sealing rings. The two sealing plates are evenly distributed around the first direction in the first chamber, and the two ends of the sealing plates along the first direction are respectively fixed to the sealing rings; the two ends of the flattening membrane along the first direction are respectively installed on the sealing plates; the two ends of the flattening membrane along the first direction are respectively installed on the sealing rings; the two sealing plates are clamped between the first side wall and the second side wall.

5. The integrated device for magnetic bead-mediated gene amplification according to claim 4, characterized in that: The first side wall and the second side wall are connected by a plurality of clamps.

6. The integrated device for magnetic bead-mediated gene amplification according to claim 1, characterized in that: A driving cavity is defined between the flattening membrane and the support plate and the second side wall. The driving member is a first air pump. The first air pump can supply air into the driving cavity or draw air from the driving cavity, so that the flattening membrane and the support plate can move along the second direction toward or away from the second side wall.

7. The integrated device for magnetic bead-mediated gene amplification according to claim 1, characterized in that: An inlet is formed on the second side wall and is located on a side of the overflow port close to the second chamber in the first direction.

8. The integrated device for magnetic bead-mediated gene amplification according to claim 1, characterized in that: An air inlet pipe is arranged on the second chamber, the air inlet pipe is communicated with the second chamber, and the air inlet pipe is externally connected to a second air pump, and the second air pump can supply air into the second chamber or extract air from the second chamber.

9. The integrated device for magnetic bead-mediated gene amplification according to claim 1, characterized in that: A balancing block is provided at the connection between the second chamber and the first chamber, and two balancing holes are provided on the balancing block. The two balancing holes are symmetrically arranged about the central axis of the balancing block in the second direction, and the balancing holes are conical. The small ends of the two balancing holes are arranged face to face and connected in the first direction.

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