Tobacco endogenous biomass-based microcapsule and preparation method thereof
By using tobacco endogenous biomass-based microcapsules to embed the fragrance in cigarette products, the problems of insufficient and unstable cigarette fragrance are solved, efficient load and stable release of fragrance are achieved, and the fragrance enhancement effect and consumer experience of cigarettes are improved.
Patent Information
- Application Number
- CN202411633349.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-15
- Publication Date
- 2025-03-21
AI Technical Summary
The existing cigarette products have insufficient aroma, unstable and prominent miscellaneous air, resulting in poor consumer smoking experience, and the volatility of tobacco flavors leads to poor fragrance enhancement effect.
By adopting the preparation method of tobacco endogenous biomass-based microcapsules, the fragrance is entrapped in the microcapsules composed of polysaccharides, tobacco endogenous biomass-based materials and curing agents, the fragrance is efficiently loaded and stable release.
It achieves efficient embedding and stable release of fragrances, improves the fragrance enhancement effect and fragrance release durability of cigarettes, and improves the consumer's suction experience.
Smart Images
Figure CN119657002A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of tobacco, and in particular relates to a tobacco endogenous biomass-based microcapsule and a preparation method thereof. Background Art
[0002] The current cigarette products are completely different from traditional cigarettes in style, such as low tar content, small smoke volume, and light taste. Therefore, the aroma is often insufficient during the smoking process, and the aroma is unstable or the smell is prominent due to differences in raw material quality, which greatly reduces the consumer's smoking experience. In order to enhance the flavor of cigarette products, cover up the smell and improve the comfort of the smoking taste, tobacco flavors can be added to cigarette products. However, most tobacco flavors are volatile, resulting in problems such as short retention time and poor flavoring effect, which restricts the improvement of cigarette smoking quality.
[0003] Nowadays, most of them appear in cigarette products in the form of flavor microcapsules, which use natural or synthetic polymer materials as wall materials to cover tobacco flavors, thereby improving the stability of the flavors, reducing volatility, and effectively covering up the impurities in cigarettes, making the smoke sweet and smooth, and making the smoke soft and delicate. However, there are few reports on the preparation of microcapsules based on tobacco endogenous biomass. For this reason, the present invention is proposed. Summary of the invention
[0004] The main purpose of the present invention is to disclose a tobacco endogenous biomass-based microcapsule and a preparation method thereof. The tobacco endogenous biomass-based flavor microcapsule of the present invention has a size of 100nm-10μm, a flavor embedding rate of more than 80%, and has a good embedding effect.
[0005] The technical solution of the present invention is as follows:
[0006] The first aspect of the present invention discloses a method for preparing tobacco endogenous biomass-based microcapsules, comprising the following steps:
[0007] S1. At a certain temperature, disperse a certain mass of polysaccharide and tobacco endogenous biomass-based material in deionized water and mix them evenly; add a certain mass of flavor, disperse and emulsify at high speed for a certain time; adjust the pH to 4.0-4.5 at a certain temperature, and continue mixing;
[0008] S2, cooling the dispersed system of S1 to below 15° C. and continuing to mix for a period of time; then heating to room temperature, adding a curing agent and performing a curing reaction for a period of time; thus, obtaining the tobacco endogenous biomass-based microcapsules.
[0009] Preferably, in step S1, the polysaccharide is one or more of gelatin, chitosan, gum arabic, cyclodextrin, and sodium alginate;
[0010] The tobacco endogenous biomass-based material is prepared from tobacco waste raw materials, has a diameter of less than 100nm, a length of 500nm-10μm, and a specific surface area of not less than 230m 2 / g; its cytotoxicity was determined by MTT method, and the cell survival rate was not less than 80%; the Zeta potential was -20~-40mV;
[0011] The flavor is a natural flavor, such as aromatics, alcohols, esters, aldehydes, fruit flavors, etc.; of course, synthetic flavors can also be used.
[0012] Preferably, in step S1, polysaccharides and tobacco endogenous biomass-based materials are wall materials, and their total concentration is (1-3) w / v%, and the mass ratio of polysaccharides to tobacco endogenous biomass-based materials is 7:1-7:4; the ratio of flavors to wall materials is 1:1-1:3.
[0013] Preferably, in step S1, the polysaccharide and tobacco endogenous biomass-based material are dispersed in deionized water at a temperature of 55-65°C for 2 to 4 hours; the high-speed dispersion and emulsification speed is not less than 10,000 rpm for 3 to 5 minutes; when the pH value is adjusted with acetic acid, the temperature is 40-50°C for 20 to 60 minutes, and the acetic acid is added slowly.
[0014] Preferably, in step S2, the curing agent is one of tannic acid and glutamyl aminotransferase.
[0015] Preferably, in step S2, the curing agent is 5-50wt% of the total mass of the wall material (polysaccharide and tobacco endogenous biomass-based material).
[0016] Preferably, in step S2, the mixing time at a temperature below 15°C is 10 to 30 minutes; the room temperature is 25-35°C, and the curing reaction time is 1 to 4 hours.
[0017] The second aspect of the present invention discloses tobacco endogenous biomass-based microcapsules prepared by the preparation method.
[0018] Preferably, the particle size is 100 nm-10 μm, and the essence embedding rate is above 80%.
[0019] The present invention has the following beneficial effects:
[0020] 1. The preparation method of tobacco endogenous biomass-based microcapsules of the present invention uses tobacco endogenous biomass-based materials as carriers and regulation to obtain microcapsules with a particle size of 100nm-10μm and an essence embedding rate of more than 80%. The diameter of the tobacco endogenous biomass-based materials used is less than 100nm, the length is 500nm-10μm, and the specific surface area is not less than 230m 2 / g; its cytotoxicity was determined by MTT method, and the cell survival rate was not less than 80%; the Zeta potential was -20 to -40mV. The present invention utilizes the tobacco endogenous biomass-based material to adjust the particle size of the flavor microcapsules, and utilizes the characteristics of the tobacco endogenous biomass-based material with high specific surface area and large aspect ratio to achieve efficient loading of tobacco flavors, and improve the flavor enhancement effect and flavor release persistence of the microcapsules. The preparation method of the present invention is simple and easy to operate with low cost, and can meet the needs of industrial production.
[0021] 2. The natural flavors, tobacco endogenous biomass-based materials and polysaccharides used in the preparation method of the present invention are all biomass materials, which are green and non-toxic. Tobacco endogenous biomass-based materials are derived from tobacco waste resources, have the characteristics of being renewable and biodegradable, reduce the dependence on petroleum-based products and minerals, have the characteristics of being low-carbon and environmentally friendly, and meet the requirements of industrial production. The tobacco endogenous biomass-based materials used in the present invention and the preparation method thereof are separately applied for patent protection by the applicant. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 The SEM images of blank microcapsules containing no tobacco endogenous biomass-based materials and microcapsules containing tobacco endogenous biomass-based materials in the present invention; (a) is the blank sample of Comparative Example 2 containing no tobacco endogenous biomass-based materials; (b) is the tobacco endogenous biomass-based microcapsules obtained in Example 2.
[0023] Figure 2 It is a SEM image of tobacco endogenous biomass-based flavor microcapsules; the mass ratio of gelatin to tobacco endogenous biomass-based materials is controlled to be 7:1, and the core-wall ratio is 1:1; the concentrations of the mixed liquid are: (a) 1% (w / v) (Example 1), (b) 2% (w / v) (Example 2), (c) 3% (w / v) (Example 7), (d) 4% (w / v) (Comparative Example 4).
[0024] Figure 3 The figure is a SEM image of tobacco endogenous biomass-based flavor microcapsules. The total concentration of gelatin and tobacco endogenous biomass-based materials is controlled to be 2% (w / v), and the core-wall ratio is 1:1; the mass ratio of gelatin to tobacco endogenous biomass-based materials is (a) 7:2 (Example 3), (b) 7:3 (Example 4), and (c) 7:4 (Comparative Example 5).
[0025] Figure 4 The SEM image of tobacco endogenous biomass-based flavor microcapsules. Flavor microcapsules: The total concentration of gelatin and tobacco endogenous biomass-based materials was controlled to be 2% (w / v), the mass ratio of gelatin to tobacco endogenous biomass-based materials was 7:1, and the core-wall ratios were (a) 1:1 (Example 2); (b) 2:1 (Example 5); (c) 3:1 (Example 6).
[0026] Figure 5 The fragrance ( Figure 5 c and Figure 5 d) and comparative example 1 does not contain fragrance ( Figure 5 a and Figure 5 b) TEM image of tobacco endogenous biomass-based microcapsules. DETAILED DESCRIPTION
[0027] The technical solution of the present invention is further described below in conjunction with specific implementation methods. It should be noted that the following examples are only used to illustrate and explain the present invention in detail, and the application scope of the present invention is not limited by the conditions in the examples.
[0028] Example 1: Preparation of tobacco endogenous biomass-based microcapsules.
[0029] S1. Dissolve a certain amount of gelatin and tobacco endogenous biomass-based materials in deionized water, with a total concentration of 1% (w / v) and a mass ratio of gelatin to tobacco endogenous biomass-based materials of 7:1, and stir at 60°C for 2h. Add a certain amount of black plum essence, with a core-to-wall ratio of 1:1, disperse and emulsify at high speed at 10000rpm for 3min, transfer to a 45°C water bath, slowly drip 10wt% acetic acid to adjust the pH to 4.4, and continue stirring at a stirring speed of 350rpm for 30min;
[0030] S2. After the pH of the above solution is stabilized, the system is quickly cooled to below 15°C using an ice water bath and stirring is continued for 30 minutes; then the system temperature is restored to room temperature, and 40wt% tannic acid curing agent (concentration is about 10wt%) of the total wall material is added to carry out curing reaction for 4 hours to obtain a tobacco endogenous biomass-based flavor microcapsule mixture.
[0031] Example 2: Preparation of tobacco endogenous biomass-based microcapsules.
[0032] S1. Dissolve a certain amount of gelatin and tobacco endogenous biomass-based materials in deionized water, the total concentration of the mixed solution is 2% (w / v), the mass ratio of gelatin to tobacco endogenous biomass-based materials is 7:1, and stir at 60°C for 2h. Add a certain amount of black plum essence, the core-wall ratio is 1:1, and emulsify at high speed at 10000rpm for 3min, then transfer to a 45°C water bath, slowly drip 10wt% acetic acid to adjust the pH to 4.4, and continue stirring at a stirring speed of 350rpm for 30min.
[0033] S2. After the pH of the above solution is stabilized, the system is rapidly cooled to below 15°C using an ice water bath and stirred for 30 minutes. Then the system temperature is restored to room temperature, and 40wt% tannic acid curing agent (concentration is about 10wt%) of the total wall material is added to perform curing reaction for 4 hours to obtain a mixture of tobacco endogenous biomass-based flavor microcapsules.
[0034] Example 3: Preparation of tobacco endogenous biomass-based microcapsules.
[0035] S1. Dissolve a certain amount of gelatin and tobacco endogenous biomass-based materials in deionized water, with a total concentration of 2% (w / v) and a ratio of gelatin to tobacco endogenous biomass-based materials of 7:2, and stir at 60°C for 2h. Add a certain amount of black plum essence, with a core-wall ratio of 1:1, and disperse and emulsify at high speed at 10000rpm for 3min, then transfer to a 45°C water bath, slowly drip 10wt% acetic acid to adjust the pH to 4.4, and continue stirring at a stirring speed of 350rpm for 30min.
[0036] S2. After the pH of the above solution is stabilized, the system is rapidly cooled to below 15°C using an ice water bath and stirred for 30 minutes. Then the system temperature is restored to room temperature, and 40wt% tannic acid curing agent (concentration is about 10wt%) of the total wall material is added to perform curing reaction for 4 hours to obtain a mixture of tobacco endogenous biomass-based flavor microcapsules.
[0037] Example 4: Preparation of tobacco endogenous biomass-based microcapsules.
[0038] S1. Dissolve a certain amount of gelatin and tobacco endogenous biomass-based materials in deionized water, with a total concentration of 2% (w / v) and a mass ratio of gelatin to tobacco endogenous biomass-based materials of 7:3, and stir at 60°C for 2 hours. Add a certain amount of black plum essence, with a core-to-wall ratio of 1:1, and disperse and emulsify at high speed at 10,000 rpm for 3 minutes, then transfer to a 45°C water bath, slowly drip 10wt% acetic acid to adjust the pH to 4.4, and continue stirring at a stirring speed of 350 rpm for 30 minutes.
[0039] S2. After the pH of the above solution is stabilized, the system is rapidly cooled to below 15°C using an ice water bath and stirred for 30 minutes. Then the system temperature is restored to room temperature, and 40wt% tannic acid curing agent (concentration is about 10wt%) of the total wall material is added to perform curing reaction for 4 hours to obtain a mixture of tobacco endogenous biomass-based flavor microcapsules.
[0040] Example 5: Preparation of tobacco endogenous biomass-based microcapsules.
[0041] S1. Dissolve a certain amount of gelatin and tobacco endogenous biomass-based materials in deionized water, with a total concentration of 2% (w / v) and a mass ratio of gelatin to tobacco endogenous biomass-based materials of 7:1, and stir at 60°C for 2 hours. Add a certain amount of black plum essence, with a core-to-wall ratio of 2:1, and disperse and emulsify at high speed at 10,000 rpm for 3 minutes, then transfer to a 45°C water bath, slowly drip 10wt% acetic acid to adjust the pH to 4.4, and continue stirring at a stirring speed of 350 rpm for 30 minutes.
[0042] S2. After the pH of the above solution is stabilized, the system is rapidly cooled to below 15°C using an ice water bath and stirred for 30 minutes. Then the system temperature is restored to room temperature, and 40wt% tannic acid curing agent (concentration is about 10wt%) of the total wall material is added to perform curing reaction for 4 hours to obtain a mixture of tobacco endogenous biomass-based flavor microcapsules.
[0043] Example 6: Preparation of tobacco endogenous biomass-based microcapsules.
[0044] S1. A certain amount of gelatin and tobacco endogenous biomass-based materials were added to deionized water with a total concentration of 2% (w / v) and a mass ratio of gelatin to tobacco endogenous biomass-based materials of 7:1. The mixture was stirred at 60°C for 2 hours. A certain amount of black plum essence was added with a core-to-wall ratio of 3:1. After high-speed dispersion and emulsification at 10,000 rpm for 3 minutes, the mixture was transferred to a 45°C water bath, 10 wt% acetic acid was slowly dripped in to adjust the pH to 4.4, and the mixture was stirred at a stirring speed of 350 rpm for 30 minutes.
[0045] S2. After the pH of the above solution is stabilized, the system is rapidly cooled to below 15°C using an ice water bath and stirred for 30 minutes. Then the system temperature is restored to room temperature, and 40wt% tannic acid curing agent (concentration is about 10wt%) of the total wall material is added to perform curing reaction for 4 hours to obtain a mixture of tobacco endogenous biomass-based flavor microcapsules.
[0046] Example 7: Preparation of tobacco endogenous biomass-based microcapsules.
[0047] S1. Dissolve a certain amount of gelatin and tobacco endogenous biomass-based materials in deionized water, the total concentration of the mixed solution is 3% (w / v), the mass ratio of gelatin to tobacco endogenous biomass-based materials is 7:1, and stir at 60°C for 2h. Add a certain amount of black plum essence, the core-wall ratio is 1:1, and disperse and emulsify at high speed at 10000rpm for 3min, then transfer to a 45°C water bath, slowly drip 10wt% acetic acid to adjust the pH to 4.4, and continue stirring at a stirring speed of 350rpm for 30min.
[0048] S2. After the pH of the above solution is stabilized, the system is rapidly cooled to below 15°C using an ice water bath and stirred for 30 minutes. Then the system temperature is restored to room temperature, and 40wt% tannic acid curing agent (concentration is about 10wt%) of the total wall material is added to perform curing reaction for 4 hours to obtain a mixture of tobacco endogenous biomass-based flavor microcapsules.
[0049] Comparative Example 1: without black plum essence, the rest is the same as Example 2.
[0050] Comparative Example 2: No tobacco endogenous biomass-based material, and other aspects are the same as in Example 2.
[0051] Comparative Example 3: without polysaccharide, other aspects are the same as Example 2.
[0052] Comparative Example 4: The total concentration of gelatin and tobacco endogenous biomass-based materials is 4% (w / v), and the rest is the same as Example 2.
[0053] Comparative Example 5: The mass ratio of gelatin to tobacco endogenous biomass-based material is 7:4, and the rest is the same as Example 2.
[0054] Product testing items are as follows:
[0055] 1. Appearance morphology observation: Scanning electron microscopy (SEM) was used to observe the morphology of microcapsules; transmission electron microscopy (TEM) was used to determine whether the microcapsules encapsulated the essence.
[0056] 2. Determination of embedding rate: accurately weigh 10 mg of black plum essence, dissolve it in anhydrous ethanol and dilute it to 10 mL to obtain 1 mg / mL black plum ethanol solution, dilute it 100 times with ethanol to 0.01 mg / mL as a stock solution, dilute the stock solution, prepare black plum ethanol solutions with concentrations of 1.0, 2.0, 4.0, 6.0, 8.0, and 10.0 μg / ml, respectively, and measure the absorbance at 280 nm to obtain the standard working curve of absorbance versus black plum concentration y=0.2924x+0.0022, where R 2 =0.9989 (y: absorbance; x: black plum concentration). Weigh 0.1g microcapsule powder sample, add 50mL anhydrous ethanol, vortex for 1min, centrifuge at 6000rpm for 5min, take the supernatant, dilute and measure its absorbance at 280nm, calculate the unembedded flavor according to the standard working curve, extract the solid obtained by centrifugation by alcohol ultrasound to obtain the embedded flavor solution, and also measure the absorbance at 280nm. Calculate the microcapsule embedding rate according to the following formula: embedding rate / %=(embedded flavor mass concentration) / (total flavor mass concentration).
[0057] The test results and analysis are as follows:
[0058] Figure 1 The SEM images of blank microcapsules without tobacco endogenous biomass-based materials and microcapsules containing tobacco endogenous biomass-based materials in the present invention; (a) is the blank sample without tobacco endogenous biomass-based materials in Comparative Example 2; (b) is the tobacco endogenous biomass-based microcapsules obtained in Example 2. Figure 1 It can be seen that the microcapsules formed in Example 2 and Comparative Example 2 are greatly different in particle size and uniformity. The microcapsules formed only by gelatin not only have large particle size but also are uneven. Figure 1(a); After adding tobacco endogenous biomass-based materials, the microcapsules formed have a small particle size, an average particle size of 500±150nm, and a uniform size. Figure 1 (b).
[0059] Figure 2 The SEM image of tobacco endogenous biomass-based flavor microcapsules; the mass ratio of gelatin to tobacco endogenous biomass-based materials was controlled to be 7:1, and the core-wall ratio was 1:1; the concentrations of the mixed solution were: (a) 1% (w / v) (Example 1), (b) 2% (w / v) (Example 2), (c) 3% (w / v) (Example 7), (d) 4% (w / v) (Comparative Example 4). Figure 2 It can be seen that the total reaction concentration has an upper limit. When the concentration is 4% (w / v), the microcapsules are not fully formed. Microcapsules with better morphology can be prepared when the concentration of tobacco endogenous biomass-based materials and gelatin mixture is 1-3% (w / v).
[0060] Figure 3 The SEM image of tobacco endogenous biomass-based flavor microcapsules. The total concentration of gelatin and tobacco endogenous biomass-based materials was controlled to be 2% (w / v), and the core-wall ratio was 1:1; the mass ratio of gelatin to tobacco endogenous biomass-based materials was (a) 7:2 (Example 3), (b) 7:3 (Example 4), and (c) 7:4 (Comparative Example 5). Figure 3 It can be seen that tobacco endogenous biomass-based materials have a good regulating effect on the particle size of microcapsule molding, but with the increase in the amount of tobacco endogenous biomass-based materials, the number of microcapsules formed decreases sharply; therefore, the addition amount of tobacco endogenous biomass-based materials must be within an appropriate range; the mass ratio of gelatin to tobacco endogenous biomass-based materials is optimal at 7:1 to 7:3.
[0061] Figure 4 This is a SEM image of tobacco endogenous biomass-based flavor microcapsules. Flavor microcapsules: The total concentration of gelatin and tobacco endogenous biomass-based materials is controlled to be 2% (w / v), the mass ratio of gelatin to tobacco endogenous biomass-based materials is 7:1, and the core-wall ratios are (a) 1:1 (Example 2); (b) 2:1 (Example 5); (c) 3:1 (Example 6). Figure 4 It can be seen that as the core-to-wall ratio increases, the number of microcapsules formed decreases. Studies have found that when the core-to-wall ratio is 1:1, the morphology of the microcapsules is the best.
[0062] Figure 5 The fragrance ( Figure 5 c and Figure 5 d) and comparative example 1 does not contain fragrance ( Figure 5 a and Figure 5 b) TEM image of tobacco endogenous biomass-based microcapsules. Figure 5It can be seen that the microcapsules of Example 2 present a solid structure after encapsulating the flavor, while the microcapsules of Comparative Example 1 which do not include the flavor present a hollow structure; this proves that under the synergistic regulation of tobacco endogenous biomass-based materials, the prepared small-particle microcapsules successfully encapsulate the flavor.
[0063] In order to explore the effect of microcapsule preparation conditions on the embedding rate of flavors, verification was conducted and summarized in Table 1. As can be seen from Table 1, when the total concentration of the mixed solution (total concentration of gelatin and tobacco endogenous biomass-based materials) and the amount of tobacco endogenous biomass-based materials are too large, the embedding rate drops sharply; the reason may be that the number of microcapsules formed drops sharply. Tobacco endogenous biomass-based materials can be regulated to form microcapsules with smaller particle sizes, which is beneficial to increase the contact surface between them and the flavors, thereby embedding more flavors.
[0064] In summary, the microcapsules obtained in Example 2 have the best overall effect; the specific conditions are: (1) the mass ratio of gelatin to tobacco endogenous biomass-based materials is 7:1; (2) the concentration of the mixed solution (total concentration of gelatin and tobacco endogenous biomass-based materials) is 2%; (3) the core-wall ratio is 1:1.
[0065] Table 1 Microcapsule embedding rate test results
[0066] Microcapsules Embedding rate (%) Example 1 85 Example 2 86 Example 3 83 Example 4 80 Example 5 87 Example 6 87 Example 7 85 Comparative Example 2 18 Comparative Example 4 15 Comparative Example 5 12
[0067] The above description is only used to introduce the specific implementation methods of the present invention in detail, but the technical solution proposed by the present invention is not limited to the above method. Without departing from the basic principles of the present technology, equivalent modifications and changes made by those skilled in the art to the technology proposed by the present invention should be included in the scope of the claims of the present invention.
Claims
1. A method for preparing tobacco endogenous biomass-based microcapsules, characterized in that: The steps include: S1. At a certain temperature, disperse a certain mass of polysaccharide and tobacco endogenous biomass-based material in deionized water and mix them evenly; add a certain mass of flavor, disperse and emulsify at high speed for a certain time; adjust the pH to 4.0-4.5 at a certain temperature, and continue mixing; S2, cooling the dispersed system of S1 to below 15° C. and continuing to mix for a period of time; then heating to room temperature, adding a curing agent and performing a curing reaction for a period of time; thus, obtaining the tobacco endogenous biomass-based microcapsules.
2. The preparation method according to claim 1, characterized in that: In step S1, the polysaccharide is one or more of gelatin, chitosan, gum arabic, cyclodextrin, and sodium alginate; The tobacco endogenous biomass-based material is prepared from tobacco waste raw materials, has a diameter of less than 100nm, a length of 500nm-10μm, and a specific surface area of not less than 230m 2 / g, cell survival rate is not less than 80%, and Zeta potential is -20~-40mV; The flavor is natural flavor.
3. The preparation method according to claim 1, characterized in that: In step S1, polysaccharide and tobacco endogenous biomass-based material are wall materials, and their total concentration is (1-3) w / v%, and the mass ratio of polysaccharide to tobacco endogenous biomass-based material is 7:1-7:4; the ratio of flavor to wall material is 1:1-1:
3.
4. The preparation method according to claim 1, characterized in that: In step S1, the polysaccharide and tobacco endogenous biomass-based material are dispersed in deionized water at a temperature of 55-65°C for 2 to 4 hours; the high-speed dispersion and emulsification speed is not less than 10,000 rpm for 3 to 5 minutes; the pH value is adjusted with acetic acid at a temperature of 40-50°C for 20 to 60 minutes.
5. The preparation method according to claim 1, characterized in that: In step S2, the curing agent is one of tannic acid and glutamyl aminotransferase.
6. The preparation method according to claim 5, characterized in that: In step S2, the curing agent is 5-50wt% of the total mass of the polysaccharide and the tobacco endogenous biomass-based material.
7. The preparation method according to claim 1, characterized in that: In step S2, the mixing time is continued for 10 to 30 minutes at a temperature below 15°C; the curing reaction time is 1 to 4 hours at a room temperature of 25 to 35°C.
8. Tobacco endogenous biomass-based microcapsules prepared according to the preparation method according to any one of claims 1 to 7.
9. The tobacco endogenous biomass-based microcapsule according to claim 8, characterized in that: The particle size is 100nm-10μm, and the essence embedding rate is above 80%.