Nutritional additive for increasing yield of sugarcane and preparation method of nutritional additive

Abstract

The preparation method comprises the following steps: preparing an enzyme 1: collecting protein casing waste, adding a preset leavening agent into the protein casing waste, carrying out fermentation treatment for 6-10 hours, sterilizing, cooling and filtering to obtain a filtrate which is the enzyme 1; preparing enzyme 2: taking couchgrass roots, adding water, pulping, separating pulp from residues, taking pulp, adding PBS mother liquor into the pulp, inoculating bacterial powder, and performing sealed fermentation for 40-52 hours to obtain the enzyme 2; preparing an enzyme 3: taking Chinese mesona herb and an alcohol solution, performing reciprocating oscillation for 5-8 hours, performing filtration, taking filtrate, diluting the filtrate by 10-20 times, then inoculating the bacterial liquid, keeping the ventilatory capacity of 0.08 m < 3 > / (m < 3 >. Min)-0.15 m < 3 > / (m < 3 >. Min), performing aerobic fermentation for 4-6 days, performing filtration, taking filtrate, and adjusting the pH value of the filtrate to 7.0-8.0 to obtain the enzyme 3; and mixing the enzyme 1, the enzyme 2 and the enzyme 3 to prepare the nutritional additive for increasing the yield of the sugarcane. Compared with the prior art, the nutrient additive containing high acid-soluble protein, flavone, organic acid and salts thereof and effective active bacteria is prepared, and the nutrient additive promotes the development of sugarcane root systems, increases the sugarcane leaf area, improves the photosynthesis rate and improves the sugarcane yield; the problems of soil degradation, nutrition imbalance and the like caused by continuous cropping of sugarcane are solved, and the soil ecology and soil fertility are restored.

Description

Technical Field

[0001] The invention relates to the technical field of enzyme processing, and in particular to a nutritional additive for increasing sugarcane yield and a preparation method thereof. Background Art

[0002] The sugarcane industry is one of the important economic pillar industries in Guangxi, and the most important link in the sugarcane industry is the production of sugarcane. The entire production process of sugarcane includes land preparation, planting, ridge breaking, fertilization, soiling, harvesting, etc. The commonly used method of sugarcane planting is perennial root planting, that is, after the sugarcane stalks on the ground are harvested in the previous planting cycle, the sugarcane buds at the base of the sugarcane pods in the ground sprout and grow into new plants under appropriate environmental conditions. Due to the characteristics of sugarcane planting and the poor harvest of other crops after the land has been planted with sugarcane, farmers often choose to plant sugarcane continuously on the soil. However, continuous planting of sugarcane has caused the yield of sugarcane per acre to decline year by year.

[0003] When sugarcane grows poorly, growers generally apply chemical fertilizers such as nitrogen fertilizer, phosphorus fertilizer, and potassium fertilizer to increase the yield of sugarcane per acre. Although the application of chemical fertilizers can increase sugarcane yield to a certain extent, the application of chemical fertilizers will consume a large amount of organic matter in the soil, aggravate nutritional imbalance, worsen soil physical and chemical properties, and reduce soil biodiversity and content. The application of chemical fertilizers in pursuit of yield will aggravate soil degradation, which will further lead to a decrease in sugarcane yield. The decrease in yield will lead to the application of more chemical fertilizers, resulting in a vicious cycle.

[0004] Agricultural enzymes are products fermented from agricultural biological resources. They have both the benefits of organic fertilizers in improving soil physical and chemical properties and the quick-acting benefits of chemical fertilizers in increasing production and improving quality. However, existing agricultural enzymes are mainly obtained through anaerobic fermentation of brown sugar, fruits and vegetables, and water in a specific proportion. The fermentation time is long and the nitrogen source content is low. At present, there is no use of animal protein waste and plants to prepare enzymes.

[0005] Research has found that during the processing of bovine collagen casings, a large amount of bovine collagen and bovine keratin waste is generated. The waste includes waste cowhide, waste cow hair, waste casings, etc., which are collectively referred to as protein casing waste. A large artificial casing processing plant produces hundreds of tons of protein casing waste every day, which is a huge amount, and the waste is difficult to be treated and degraded through environmental protection facilities. Therefore, the utilization of protein casing waste is an urgent problem to be solved. Summary of the invention

[0006] The invention provides a nutrient additive for increasing sugarcane yield, aiming to overcome the problem that the existing continuous sugarcane cropping with more chemical fertilizers to increase yield aggravates the decline of organic matter and soil degradation, thereby aggravating the decline of sugarcane yield.

[0007] The present invention also provides a method for preparing a nutritional additive for increasing sugarcane production, aiming to solve the problem that protein casing waste is difficult to be treated and degraded by environmental protection facilities, and to overcome the defect of not utilizing animal protein waste and special plant resources to prepare enzymes containing high acid-soluble proteins, flavonoids, small molecular organic acids and effective active bacteria, so as to improve the effect of the nutritional additive on increasing sugarcane production.

[0008] To achieve the above object, the present invention provides a method for preparing a nutritional additive for increasing sugarcane production, the method comprising:

[0009] S1, making enzyme 1: collecting protein casing waste, adding a preset fermentation agent for fermentation for 6-10 hours, sterilizing, cooling and filtering, and the filtrate is enzyme 1;

[0010] S2, making enzyme 2: taking Imperata root and beating it with water, separating the pulp residue, taking the pulp, adding PBS mother solution to the pulp, and then inoculating bacterial powder, and fermenting it for 40-52 hours in a sealed manner to obtain enzyme 2;

[0011] S3, make enzyme 3: take the agar grass and alcohol solution, reciprocate and oscillate for 5-8 hours, filter and dilute the filtrate 10-20 times, then inoculate the bacterial solution, and maintain the ventilation volume at 0.08m 3 / (m 3 ·min)-0.15m 3 / (m 3 ·min), aerobic fermentation for 4-6 days, filtering the filtrate to adjust the pH to 7-8.0, and obtaining enzyme 3;

[0012] S4, mixing enzyme 1, enzyme 2 and enzyme 3 to prepare a nutritional supplement for increasing sugarcane production.

[0013] Preferably, in the above technical solution, in the step of fermentation treatment with a preset starter, the preset starter is one or more of Bacillus subtilis dry powder, Bacillus amyloliquefaciens dry powder, alkaline protease and neutral protease.

[0014] Preferably, in the above technical solution, the preset leavening agent is 0.5-1‰ Bacillus subtilis dry powder, 0.5-1‰ Bacillus amyloliquefaciens dry powder, 1-2‰ alkaline protease and 1-2‰ neutral protease.

[0015] Preferably, in the above technical scheme, in step S1, the specific method for preparing enzyme 1 is: collecting protein casing waste, adding quicklime to the protein casing waste, heating and boiling and maintaining for 2-4 hours, when cooled to 45-55°C, adding a preset fermentation agent and fermenting for 6-10 hours, sterilizing, cooling and filtering, and the filtrate is enzyme 1.

[0016] Preferably, in the above technical solution, the bacterial powder is 0.5-1‰ Lactobacillus plantarum dry powder and 0.5-1‰ Pediococcus dry powder.

[0017] Preferably, in the above technical solution, in step S3, the bacterial solution is 2-3% Acetobacter pasteurianus bacterial solution.

[0018] Preferably, in the above technical solution, the pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria in acetic acid bacteria culture liquid, and the acetic acid bacteria culture liquid is mixed by 10-15g glucose, 10-15g yeast extract, 8-10g calcium carbonate, 2.5-5g sodium acetate, 1.5-3g calcium lactate and 1000-120mL PBS, sterilized at 115-121°C for 30-40min, and then cooled and 50-60mL alcohol is added and mixed evenly.

[0019] Preferably, in the above technical solution, the aerobic fermentation refers to maintaining 0.08m 3 / (m 3 ·min)-0.15m 3 / (m 3 ·min) oxygen flow rate.

[0020] Preferably, in the above technical solution, in step S4, the nutritional additive is prepared by mixing 20-60 parts of enzyme 1, 10-40 parts of enzyme 2 and 10-40 parts of enzyme 3.

[0021] A nutritional additive for increasing sugarcane yield prepared by the preparation method as described above.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The nutritional additive of the present invention has the following principle of promoting sugarcane yield increase: (1) utilizing Bacillus subtilis, Bacillus amyloliquefaciens, alkaline protease and neutral protease to synergistically promote the degradation of protein casing waste into acid-soluble protein, and the acid-soluble protein, flavonoid content, organic acid and its salts jointly promote the development of sugarcane root system, increase sugarcane leaf area and improve photosynthesis rate, so as to increase sugarcane yield; (2) before fermenting protein casing waste, quicklime and high-temperature boiling are used to neutralize the acidity of protein casing waste and promote the denaturation of protein casing waste, thereby providing a more suitable fermentation environment for fermented bacteria and enzyme agents, so as to increase the fermentation speed; (3) fermenting Imperata cylindrica and Agar-agar with bacteria increases the flavonoid content and effective active bacteria content and their metabolites in the nutritional additive, and synergizing with acid-soluble protein is conducive to the effective active bacteria forming a stable bacterial community in the soil, thereby increasing the soil biological content and promoting the improvement of soil structure. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] A method for preparing a nutritional additive for increasing sugarcane production, the method comprising:

[0027] S1, making enzyme 1: collecting protein casing waste, adding 2% quicklime to the protein casing waste, heating and boiling and keeping for 4 hours to obtain a fermented liquid, when the fermented liquid is cooled to 45°C, adding 0.5‰ Bacillus subtilis dry powder, 0.5‰ Bacillus amyloliquefaciens dry powder, 1‰ alkaline protease, 1‰ neutral protease to the fermented liquid, stirring and fermenting for 8 hours, heating to 100°C after the fermentation is completed, keeping for 30 minutes, filtering, and the filtrate is enzyme 1;

[0028] S2, making enzyme 2: taking 10 parts of Imperata root and 90 parts of water to make pulp, separating the pulp residue, taking the pulp, adding 1‰ PBS mother solution to the pulp, stirring evenly, adding 0.5‰ Lactobacillus plantarum powder and 0.5‰ Pediococcus dry powder, stirring evenly, and anaerobically fermenting at 36°C for 48h to obtain enzyme 2;

[0029] Preparation of PBS stock solution: weigh 34.0 g potassium dihydrogen phosphate and dissolve it in 500 mL distilled water. Use about 175 mL of 1 mol / L sodium hydroxide solution to adjust the pH to 7.2. Dilute to 1000 mL with distilled water and store in a refrigerator. Dilute the stock solution 1000 times before use.

[0030] S3, make enzyme 3: take 10 parts of agar grass and 100 parts of 60% alcohol solution, reciprocate and shake for 8 hours, filter and take the filtrate, dilute it 20 times, inoculate 2% pasteurized acetic acid bacteria solution and stir well, keep the oxygen flow rate at 0.08m 3 / (m 3 ·min)-0.15m 3 / (m 3 ·min), aerobic fermentation for 6 days, filtering after fermentation, adjusting the pH of the filtrate to 8.0 with 1 mol / L potassium hydroxide solution, and obtaining enzyme 3;

[0031] The pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria into acetic acid bacteria culture liquid. The preparation method of acetic acid bacteria culture liquid is as follows: 10g of glucose, 10g of yeast extract, 8g of calcium carbonate, 2.5g of sodium acetate, 1.5g of calcium lactate and 1000mL of PBS, sterilized at 115°C for 30min, and after cooling, 50mL of alcohol is added and mixed evenly;

[0032] S4, mixing 60 parts of the enzyme 1, 40 parts of the enzyme 2 and 40 parts of the enzyme 3 to prepare a nutritional additive for increasing sugarcane production.

[0033] Example 2

[0034] A method for preparing a nutritional additive for increasing sugarcane production, the method comprising:

[0035] S1, making enzyme 1: collecting protein casing waste, adding 2% quicklime to the protein casing waste, heating and boiling and keeping for 2 hours to obtain a fermented liquid, when the fermented liquid is cooled to 55°C, adding 1‰ Bacillus subtilis dry powder, 1‰ Bacillus amyloliquefaciens dry powder, 2‰ alkaline protease, 2‰ neutral protease to the fermented liquid, stirring and fermenting for 10 hours, heating to 100°C after the fermentation, keeping for 40 minutes, filtering, and the filtrate is enzyme 1;

[0036] S2, making enzyme 2: taking 10 parts of Imperata root and 90 parts of water to make pulp, separating the pulp residue, taking the pulp, adding 1‰ PBS mother solution to the pulp, stirring evenly, adding 1‰ Lactobacillus plantarum powder and 1‰ Pediococcus dry powder, stirring evenly, and anaerobically fermenting at 36°C for 48h to obtain enzyme 2;

[0037] Preparation of PBS stock solution: weigh 34.0 g potassium dihydrogen phosphate and dissolve it in 500 mL distilled water. Use about 175 mL of 1 mol / L sodium hydroxide solution to adjust the pH to 7.2. Dilute to 1000 mL with distilled water and store in a refrigerator. Dilute the stock solution 1000 times before use.

[0038] S3, make enzyme 3: take 10 parts of agar grass and 100 parts of 60% alcohol solution, reciprocate and shake for 8 hours, filter and take the filtrate, dilute it 10-20 times, inoculate 3% pasteurized acetic acid bacteria solution and stir well, keep the oxygen flow rate at 0.08m 3 / (m 3 ·min)-0.15m 3 / (m 3 ·min), aerobic fermentation for 5 days, after fermentation, the pH of the fermentation liquid was adjusted to 7.0 with 1 mol / L potassium hydroxide solution to obtain enzyme 3;

[0039] The pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria into acetic acid bacteria culture liquid. The preparation method of acetic acid bacteria culture liquid is as follows: 10g of glucose, 10g of yeast extract, 8g of calcium carbonate, 2.5g of sodium acetate, 1.5g of calcium lactate and 1000mL of PBS, sterilized at 115°C for 30min, and after cooling, 50mL of alcohol is added and mixed evenly;

[0040] S4, mixing 20 parts of the enzyme 1, 10 parts of the enzyme 2 and 40 parts of the enzyme 3 to prepare a nutritional additive for increasing sugarcane production.

[0041] Example 3

[0042] A method for preparing a nutritional additive for increasing sugarcane production, the method comprising:

[0043] S1, making enzyme 1: collecting protein casing waste, breaking it, adding 2% quicklime to the protein casing waste, heating and boiling and keeping it for 4 hours to obtain a fermented liquid, when the fermented liquid is cooled to 55°C, adding 0.5‰ Bacillus subtilis dry powder, 0.5‰ Bacillus amyloliquefaciens dry powder, 1‰ alkaline protease, 1‰ neutral protease to the fermented liquid, stirring and fermenting for 10 hours, heating to 100°C after the fermentation, keeping it for 40 minutes, filtering, and the filtrate is enzyme 1;

[0044] S2, making enzyme 2: taking 10 parts of Imperata root and 90 parts of water to make pulp, separating the pulp residue, taking the pulp, adding 1‰ PBS mother solution to the pulp, stirring evenly, adding 0.5‰ Lactobacillus plantarum powder and 0.5‰ Pediococcus dry powder, stirring evenly, and anaerobically fermenting at 36°C for 48h to obtain enzyme 2;

[0045] Preparation of PBS stock solution: weigh 34.0 g potassium dihydrogen phosphate and dissolve it in 500 mL distilled water. Use about 175 mL of 1 mol / L sodium hydroxide solution to adjust the pH to 7.2. Dilute to 1000 mL with distilled water and store in a refrigerator. Dilute the stock solution 1000 times before use.

[0046] S3, make enzyme 3: take 10 parts of agar grass and 100 parts of 60% alcohol solution, reciprocate for 5 hours, filter the filtrate, dilute it 10-20 times, inoculate 2% pasteurized acetic acid bacteria solution, stir well, and maintain the oxygen flow rate at 0.08m 3 / (m 3 ·min)-0.15m 3 / (m 3 ·min), aerobic fermentation for 6 days, after fermentation, the pH of the fermentation liquid was adjusted to 8.0 with 1 mol / L potassium hydroxide solution to obtain enzyme 3;

[0047] The pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria into acetic acid bacteria culture liquid. The preparation method of acetic acid bacteria culture liquid is as follows: 10g of glucose, 10g of yeast extract, 8g of calcium carbonate, 2.5g of sodium acetate, 1.5g of calcium lactate and 1000mL of PBS, sterilized at 115°C for 30min, and then cooled and mixed with 50mL of alcohol;

[0048] S4, mixing 20 parts of the enzyme 1, 20 parts of the enzyme 2 and 30 parts of the enzyme 3 to prepare a nutritional additive for increasing sugarcane production.

[0049] Example 4

[0050] A method for preparing a nutritional additive for increasing sugarcane production, the method comprising:

[0051] S1, making enzyme 1: collecting protein casing waste, breaking it, adding 2% quicklime to the protein casing waste, heating and boiling and keeping it for 2 hours to obtain a fermented liquid, when the fermented liquid is cooled to 45°C, adding 1‰ Bacillus subtilis dry powder, 1‰ Bacillus amyloliquefaciens dry powder, 2‰ alkaline protease, 2‰ neutral protease to the fermented liquid, stirring and fermenting for 10 hours, heating to 100°C after the fermentation, keeping it for 40 minutes, filtering, and the filtrate is enzyme 1;

[0052] S2, making enzyme 2: taking 10 parts of Imperata root and 90 parts of water to make pulp, separating the pulp residue, taking the pulp, adding 1‰ PBS mother solution to the pulp, stirring evenly, adding 0.5‰ Lactobacillus plantarum powder and 0.5‰ Pediococcus dry powder, stirring evenly, and anaerobically fermenting at 36°C for 48h to obtain enzyme 2;

[0053] Preparation of PBS stock solution: weigh 34.0 g potassium dihydrogen phosphate and dissolve it in 500 mL distilled water. Use about 175 mL of 1 mol / L sodium hydroxide solution to adjust the pH to 7.2. Dilute to 1000 mL with distilled water and store in a refrigerator. Dilute the stock solution 1000 times before use.

[0054] S3, make enzyme 3: take 10 parts of agar grass and 100 parts of 60% alcohol solution, reciprocate and shake for 8 hours, filter and take the filtrate, dilute it 10-20 times, inoculate 3% pasteurized acetic acid bacteria solution and stir well, keep the oxygen flow rate at 0.08m 3 / (m 3 ·min)-0.15m 3 / (m 3 ·min), aerobic fermentation for 6 days, after fermentation, the pH of the fermentation liquid was adjusted to 8.0 with 1 mol / L potassium hydroxide solution to obtain enzyme 3;

[0055] The pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria into acetic acid bacteria culture liquid. The preparation method of acetic acid bacteria culture liquid is as follows: 10g of glucose, 10g of yeast extract, 8g of calcium carbonate, 2.5g of sodium acetate, 1.5g of calcium lactate and 1000mL of PBS, sterilized at 115°C for 30min, and after cooling, 50mL of alcohol is added and mixed evenly;

[0056] S4, mixing 40 parts of the enzyme 1, 20 parts of the enzyme 2 and 20 parts of the enzyme 3 to prepare a nutritional additive for increasing sugarcane production.

[0057] Comparative Example 1

[0058] Preparation of enzyme 1: Collect protein casing waste, add 0.5‰ Bacillus subtilis powder, 0.5‰ Bacillus amyloliquefaciens powder, 1‰ alkaline protease, and 1‰ neutral protease to the protein casing waste, stir well and ferment for 12 hours. After the fermentation is completed, heat to 100°C, maintain for 30 minutes, filter, and the filtrate is enzyme 1.

[0059] Comparative Example 2

[0060] Preparation of enzyme 1: Collect protein casing waste, add 1% quicklime to the protein casing waste, heat and boil for 2-4 hours to obtain the fermented liquid, when the fermented liquid is cooled to 45-55°C, add 1‰ alkaline protease and 1‰ neutral protease to the fermented liquid, stir well and ferment for 8 hours, raise the temperature to 100°C after fermentation, keep for 30 minutes, filter, and the filtrate is enzyme 1.

[0061] Comparative Example 3

[0062] Preparation of enzyme 1: Collect protein casing waste, add 2% quicklime to the protein casing waste, heat and boil and keep for 2-4 hours to obtain the fermented liquid, when the fermented liquid is cooled to 45-55°C, add 0.5‰ Bacillus subtilis powder and 0.5‰ Bacillus amyloliquefaciens powder to the fermented liquid, stir well and ferment for 8 hours, raise the temperature to 100°C after the fermentation, keep for 30 minutes, filter, and the filtrate is enzyme 1.

[0063] The acid-soluble protein content of Example 1 and Comparative Examples 1-3 after fermentation for 6 h, 8 h, 10 h and 12 h is shown in Table 1 below.

[0064] Table 1 Changes in acid-soluble protein content of enzyme 1 in Example 1 and Comparative Examples 1-3 as fermentation time increases

[0065] Group 6h 8h 10h 12h Example 1 20.6 25.8 25.8 25.9 Comparative Example 1 1.5 1.9 2.7 3.5 Comparative Example 2 6.7 7.6 9.4 12.2 Comparative Example 3 7.3 9.1 12.9 15.6

[0066] As can be seen from Table 1, under the same fermentation time, compared with Example 1, the content of acid-soluble protein in Comparative Examples 1, Comparative Examples 2 and Comparative Examples 3 is higher, indicating that under the fermentation process conditions of the present invention, it is conducive to the degradation of protein casing waste by enzymes or bacterial agents into acid-soluble proteins. The principle may be that the protein casing waste is weakly acidic and alkaline in quicklime solution. Under alkaline heating conditions, it undergoes alkaline hydrolysis reaction with the protein carboxyl groups in the protein casing waste to form smaller compounds, while changing the protein spatial conformation, increasing the contact area and contact sites between bacteria and enzymes, and accelerating the decomposition rate of protein casing waste. At the same time, the pH value of the protein casing waste is increased, and no additional alkali solution is required to adjust the pH value, reducing the fermentation steps and costs. And Bacillus subtilis dry powder, Bacillus amyloliquefaciens dry powder, alkaline protease and neutral protease are used together to implement bacterial enzyme synergistic degradation, and the degradation rate of protein casing waste is faster.

[0067] After fermentation for 10 hours in comparative examples 1-3, enzyme 1 was obtained respectively. The nutritional components of enzyme 1 are as follows:

[0068]

[0069] It can be seen from the above table that the organic acids and salts thereof in Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 are almost zero, because the protein casing waste contains almost no sugar substances; the pH values ​​of Comparative Examples 1 and 2 are lower than those of Example 1 and Comparative Example 3, because the percentages of quicklime added are different.

[0070] After fermenting comparative examples 1-3 for 10 hours, enzyme 1 was obtained respectively, and mixed with enzyme 2 and enzyme 3 prepared by the following method. The preparation method of enzyme 2 is as follows:

[0071] Preparation of enzyme 2: 10 parts of Imperata root and 90 parts of water were slurried, and the slurry was separated from the slurry residue. 1‰ PBS mother solution was added to the slurry, and stirred evenly. 0.5‰ Lactobacillus plantarum powder and 0.5‰ Pediococcus powder were added and stirred evenly. The mixture was anaerobically fermented at 36°C for 48 hours to obtain enzyme 2.

[0072] Preparation of PBS stock solution: weigh 34.0 g potassium dihydrogen phosphate and dissolve it in 500 mL distilled water. Use about 175 mL of 1 mol / L sodium hydroxide solution to adjust the pH to 7.2. Dilute to 1000 mL with distilled water and store in a refrigerator.

[0073] The preparation method of the enzyme 3 is as follows:

[0074] Preparation of enzyme 3: Take 10 parts of agar grass and 100 parts of 60% alcohol solution, shake back and forth for 5-8 hours, filter the filtrate, dilute it 10-20 times, inoculate 2% pasteurized acetic acid bacteria solution, stir well, and maintain the ventilation volume at 0.08m 3 / (m 3·min)-0.15m 3 / (m 3 ·min), aerobic fermentation for 6 days, filtering after fermentation, adjusting the pH of the filtrate to 8.0 with 1 mol / L potassium hydroxide solution, and obtaining enzyme 3;

[0075] The pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria into acetic acid bacteria culture liquid. The preparation method of acetic acid bacteria culture liquid is as follows: 10g of glucose, 10g of yeast extract, 8g of calcium carbonate, 2.5g of sodium acetate, 1.5g of calcium lactate and 1000mL of PBS, sterilized at 115°C for 30min, and after cooling, 50mL of alcohol is added and mixed evenly;

[0076] Mixing 60 parts of the enzyme 1 prepared in comparative example 1, 40 parts of the enzyme 2 and 40 parts of the enzyme 3 to prepare a nutritional additive for increasing sugarcane production;

[0077] 60 parts of the enzyme 1 prepared in comparative example 2, 40 parts of the enzyme 2 and 40 parts of the enzyme 3 are mixed to prepare a nutritional additive for increasing sugarcane production;

[0078] 60 parts of the enzyme 1 prepared in comparative example 3, 40 parts of the enzyme 2 and 40 parts of the enzyme 3 were mixed to prepare a nutritional additive for increasing sugarcane production.

[0079] Comparative Example 4

[0080] Preparation of enzyme 2: Take 10 parts of Imperata root and add 90 parts of water to pulp, separate the pulp and residue, take the slurry, add 1‰ PBS mother liquor to the slurry, stir evenly, add 0.5‰ Lactobacillus plantarum powder and 0.5‰ Bacillus amyloliquefaciens powder, stir evenly, and anaerobically ferment at 36°C for 48 hours to obtain enzyme 2.

[0081] Comparative Example 5

[0082] Preparation of enzyme 3: Take 10 parts of agar grass and 100 parts of 60% alcohol solution, shake back and forth for 5-8 hours, filter the filtrate, dilute it 10-20 times, inoculate 2% pasteurized acetic acid bacteria solution, stir well, and maintain the ventilation volume at 0.01m 3 / (m 3 ·min)-0.05m 3 / (m 3 ·min), aerobic fermentation for 6 days, filtration after fermentation, and enzyme 3;

[0083] The pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria into acetic acid bacteria culture liquid. The preparation method of acetic acid bacteria culture liquid is as follows: 10g of glucose, 10g of yeast extract, 8g of calcium carbonate, 2.5g of sodium acetate, 1.5g of calcium lactate, 1000mL of PBS, sterilized at 115℃ for 30min, and adding 50mL of alcohol after cooling.

[0084] Comparative Example 6

[0085] S1, collecting protein casing waste, adding 2% quicklime to the protein casing waste, heating and boiling and maintaining for 2-4 hours to obtain a fermentation liquid;

[0086] S2, taking 10 parts of Imperatae rhizome and 10 parts of Agarwood, adding 100 parts of water to pulp, separating the pulp and residue, and taking the pulp;

[0087] S3, mixing the fermented liquid with the slurry, adding 0.5‰ Bacillus subtilis dry powder, 0.5‰ Bacillus amyloliquefaciens dry powder, 1‰ alkaline protease, 1‰ neutral protease, 0.5‰ Lactobacillus plantarum dry powder and 0.5‰ Pediococcus dry powder, stirring well and fermenting for 8 days, raising the temperature to 100°C after the fermentation is completed, maintaining for 30 minutes, filtering, and the filtrate is a nutritional additive for increasing sugarcane production.

[0088] According to the industry standard QB / T5323-2018, the nutritional components of Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5 and Comparative Example 6 were tested, and the nutritional components are shown in Table 2 below.

[0089] Table 2 Nutritional composition of the nutritional supplements in each specific embodiment

[0090]

[0091] As can be seen from the table above, compared with Example 1, the flavonoid content of Comparative Examples 1-3 is not much different, indicating that the flavonoids mainly come from enzyme 2 and enzyme 3. The enzyme 2 and enzyme 3 obtained by the same fermentation method and fermentation conditions as Example 1 may have slight changes due to the activity of the bacterial agent or the raw material conditions, but the changes are not large. The organic acids and their salts change greatly. For example, the organic acids and their salts in Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 increase from zero to more than 800. The reason is that enzyme 1 and enzyme 3 contain more organic acids and their salts. Compared with Comparative Example 6, Example 1 ferments the protein casing waste, Imperata root and Agar grass separately and then mixes them. Each enzyme is fermented more completely by the bacterial agent or enzyme agent to obtain more acid-soluble protein, flavonoids, organic acids and effective viable bacteria.

[0092] According to the industry standard QB / T5323-2018, the heavy metal contents of Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5 and Comparative Example 6 were tested, and the heavy metal contents were shown in Table 3. Among them, the heavy metal detection includes the total amount of arsenic and its compounds (As), the total amount of cadmium and its compounds (Cd), the total amount of lead and its compounds (Pb), the total amount of chromium and its compounds (Cr), and the total amount of mercury and its compounds (Hg).

[0093] Table 3 Heavy metal content of nutritional supplements in each specific embodiment

[0094]

[0095]

[0096] The nutritional supplements prepared in Examples 1-3 and Comparative Examples 1-6 were applied to the sugarcane fields of the corresponding test plots, respectively, and the application method was: 4 times per sugarcane planting cycle, 1 kg / mu each time, and a total application amount of 4 kg / mu, diluted 200 times with water before spraying or drip irrigation. At harvest, the yield of sugarcane in each test plot is shown in Table 4 below.

[0097] Table 4 Sugarcane yield after applying nutritional additives

[0098] Group Yield (kg / mu) Example 1 6560 Example 2 6810 Example 3 6550 Example 4 6680 Comparative Example 1 4780 Comparative Example 2 5120 Comparative Example 3 5360 Comparative Example 4 4130 Comparative Example 5 4270 Comparative Example 6 5730

[0099] After harvest, the soil nutrient conditions of the plots using the nutritional supplement prepared by the present invention and the plots where compound fertilizers were conventionally applied were compared, and the results are shown in Table 5 below.

[0100] Table 5 Soil nutrients of soil blocks with nutritional additives and fertilizers

[0101] Yield (kg / mu) Organic matter (g / kg) <![CDATA[Bacteria (1×10 6 CFU / g)]]> Nutritional additives 6560 31.9 139.67 Compound Fertilizer 5970 20.7 86.55

[0102] The present invention can be implemented in various ways and is not limited to the embodiments described. A person skilled in the art can understand that the present invention can be implemented in other specific ways without changing the technical concept or essential features of the present invention. Therefore, it should be understood that the embodiments described above are exemplary and are not intended to limit the present invention.

Claims

1. A method for preparing a nutritional additive for increasing sugarcane production, characterized in that: include: S1, making enzyme 1: collecting protein casing waste, adding a preset fermentation agent for fermentation for 6-10 hours, sterilizing, cooling and filtering, and the filtrate is enzyme 1; S2, making enzyme 2: taking Imperata root and beating it with water, separating the pulp residue, taking the pulp, adding PBS mother solution to the pulp, and then inoculating bacterial powder, and fermenting it for 40-52 hours to obtain enzyme 2; S3, making enzyme 3: taking agar grass and alcohol solution, reciprocatingly oscillating for 5-8 hours, filtering and diluting the filtrate by 10-20 times, then inoculating the bacterial solution, aerobically fermenting for 4-6 days, filtering and adjusting the filtrate to pH 7.0-8.0, and obtaining enzyme 3; S4, mixing enzyme 1, enzyme 2 and enzyme 3 to prepare a nutritional supplement for increasing sugarcane production.

2. The method for preparing a nutritional additive for increasing sugarcane production according to claim 1, characterized in that: In the step of fermentation treatment with a preset starter, the preset starter is one or more of Bacillus subtilis dry powder, Bacillus amyloliquefaciens dry powder, alkaline protease and neutral protease.

3. The method for preparing a nutritional additive for increasing sugarcane production according to claim 2, characterized in that: The preset fermentation agent is 0.5-1‰ Bacillus subtilis dry powder, 0.5-1‰ Bacillus amyloliquefaciens dry powder, 1-2‰ alkaline protease and 1-2‰ neutral protease.

4. The method for preparing a nutritional additive for increasing sugarcane production according to claim 1, characterized in that: In step S1, the specific method for preparing enzyme 1 is: collecting protein casing waste, adding quicklime to the protein casing waste, heating and boiling and maintaining for 2-4 hours, when cooled to 45-55°C, adding a preset fermentation agent and fermenting for 6-10 hours, sterilizing, cooling and filtering, and the filtrate is enzyme 1.

5. The method for preparing a nutritional additive for increasing sugarcane production according to claim 1, characterized in that: The bacterial powder is 0.5-1‰ Lactobacillus plantarum dry powder and 0.5-1‰ Pediococcus dry powder.

6. The method for preparing a nutritional additive for increasing sugarcane production according to claim 1, characterized in that: In step S3, the bacterial solution is 2-3% Acetobacter pasteurianus bacterial solution.

7. The method for preparing a nutritional additive for increasing sugarcane production according to claim 6, characterized in that: The pasteurized acetic acid bacteria liquid is obtained by inoculating pasteurized acetic acid bacteria into acetic acid bacteria culture liquid, and the acetic acid bacteria culture liquid is prepared by mixing 10-15g of glucose, 10-15g of yeast extract, 8-10g of calcium carbonate, 2.5-5g of sodium acetate, 1.5-3g of calcium lactate and 1000-120mL of PBS, sterilizing at 115-121°C for 30-40min, and adding 50-60mL of alcohol after cooling and mixing evenly.

8. The method for preparing a nutritional additive for increasing sugarcane production according to claim 1, characterized in that: The aerobic fermentation refers to maintaining 0.08m 3 / (m 3 ·min)-0.15m 3 / (m 3 ·min) oxygen flow rate.

9. The method for preparing a nutritional additive for increasing sugarcane production according to claim 1, characterized in that: In step S4, the nutritional supplement is prepared by mixing 20-60 parts of enzyme 1, 10-40 parts of enzyme 2 and 10-40 parts of enzyme 3.

10. A nutritional supplement for increasing sugarcane production obtained by the preparation method according to claims 1 to 9.

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