Sealing cover for balancing pressure in multiple reagent bins and test box

Abstract

The invention discloses a sealing cover for balancing pressure in a plurality of reagent bins and a test box. The sealing cover comprises a first cover body, a second cover body, x first sealing structures, a second sealing structure and a third sealing structure, the first cover body is used for being combined with the box body in a sealing mode, and the second cover body covers the first cover body in an openable mode; the first cover body is provided with a first surface, the first surface is provided with an assembly hole, a first operation hole and x air holes which penetrate through the first cover body, the x air holes are distributed around the first operation hole in a surrounding mode, the x first sealing structures are fixedly arranged on the first surface, each first sealing structure covers one air hole, and the x first sealing structures are fixedly arranged on the first surface. The first sealing structure is air-permeable and liquid-impermeable; the second cover body is provided with a second face, a second operation hole penetrating through the second cover body is formed in the second face, and a pressing column is further arranged on the second face.

Description

Technical Field

[0001] The present invention particularly relates to a sealing cover and a test box for balancing the pressure in a plurality of reagent chambers, and belongs to the technical field of biological monitoring devices. Background Art

[0002] The development of microfluidic technology can provide more stable and mass-producible technical solutions for fully integrated nucleic acid testing products. Taking GeneXpert (Chinese name: Cepei) as the performance benchmark, it can truly enter the POCT scenario to solve clinical pain points.

[0003] For POCT nucleic acid testing, the core is that the entire testing process is completed in the internal chamber of the cartridge to avoid cross contamination to the greatest extent. Cross contamination refers to contamination between samples and aerosol contamination. The testing process is carried out in a closed manner to ensure safe operation.

[0004] Independent test boxes reduce pollution and environmental interference. Integrating sample preparation, amplification, and quantitative detection in one box also brings up a problem: how to exhaust and intake air between the chambers inside each card box.

[0005] Take the Cepec lid as an example. After opening the lid, the topmost sealing film and the sealing port fall off. If the cartridge is inverted at this time, there will be leakage. In addition, the extraction process is to exhaust air outwards, and the amplification product in the last step is sealed in the cartridge. Although the contamination of the amplified product is reduced, there is a risk of sample aerosol contamination.

[0006] The card boxes on the market have independent sealing between each compartment, and the temperature can be kept constant by refrigerated trucks without causing the pressure in the compartment to increase. If it is transported by sea, when the temperature in the container reaches 50-60℃, the pressure between the individual compartments will increase, and the sealing film of heat pressing or ultrasonic welding will easily crack, causing leakage, making the detection inaccurate. Summary of the invention

[0007] The main purpose of the present invention is to provide a sealing cover and a test box for balancing the pressure in multiple reagent chambers, thereby overcoming the deficiencies in the prior art.

[0008] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention includes: A first aspect of an embodiment of the present invention provides a sealing cover for balancing the pressure in multiple reagent chambers, comprising: a first cover body, a second cover body, x first sealing structures, a second sealing structure, and a third sealing structure, wherein the first cover body is used to be sealed and combined with a box body, and the second cover body is openably covered on the first cover body; The first cover body has a first surface, on which a mounting hole, a first operating hole and x air holes are arranged penetrating the first cover body, the x air holes are distributed around the first operating hole, and x first sealing structures are fixedly arranged on the first surface, each of the first sealing structures covers an air hole, and the first sealing structure is permeable to air but not permeable to liquid; the second cover body has a second surface, on which a second operating hole penetrating the second cover body is arranged, and a pressure column is also arranged on the second surface; When the second cover body is covered on the first cover body, the first cover body is sealed and combined with the second cover body, and the first surface and the second surface enclose a sandwich space isolated from the external environment, the first operating hole corresponds to and is connected with the second operating hole, and the pressure column corresponds to the assembly hole; the second sealing structure and the third sealing structure are arranged between the first surface and the second surface, the second sealing structure is arranged between the first operating hole and the x first sealing structures, the second sealing structure is arranged around the periphery of the first operating hole and the second operating hole, and the third sealing structure is arranged around the periphery of the x first sealing structures, the second sealing structure and the third sealing structure are sealed and matched with the first surface and the second surface, the second sealing structure, the third sealing structure, the first surface and the second surface jointly enclose a closed ventilation space, the x first sealing structures are encapsulated in the ventilation space, and the air vent can exchange gas with the ventilation space through the first sealing structure, x≥2.

[0009] The second aspect of an embodiment of the present invention provides a test box, which includes: a box body and a sealing cover for balancing the pressure in multiple reagent chambers, the box body has x reagent chambers, a sample tube chamber and a negative pressure chamber, the x reagent chambers are isolated from each other, the sealing cover is arranged on the box body, the first cover body is sealed and combined with the box body, the x air holes correspond to the x reagent chambers one by one, the first operating hole corresponds to and is connected to the negative pressure chamber, and the assembly hole corresponds to and is connected to the sample tube chamber.

[0010] Compared with the prior art, the advantages of the present invention include: A test kit provided by an embodiment of the present invention can place the entire system of the amplification reaction (including liquid) in each functional chamber for sealing operation, thereby avoiding contamination of the operating space environment, reducing false positives, and improving detection accuracy; In a test kit provided by an embodiment of the present invention, the pressure in each reagent chamber does not affect the liquid transfer; The sealing cover in a test box provided by an embodiment of the present invention has double sealing protection, reduces the risk of liquid leakage, can ensure safety in different transportation environments, and can meet the transportation environment under high temperature; The sealing cover in the test box provided by the embodiment of the present invention has a simple structure, does not require hot pressing, and can protect reagents or freeze-dried balls that are sensitive to temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0012] Figure 1 It is a structural schematic diagram of a test box in a closed state provided in a typical implementation case of the present invention; Figure 2 It is a structural schematic diagram of a second cover body of a sealing cover in a test box provided in a typical implementation case of the present invention in an open state; Figure 3 It is a structural schematic diagram of a second cover body of a sealing cover in a test box provided in a typical implementation case of the present invention in an open state; Figure 4 It is a side view of a second cover body of a sealing cover in a test box provided in a typical embodiment of the present invention in an open state; Figure 5 It is a schematic diagram of an exploded structure of a first sealing structure in which a second cover body of a sealing cover in a test box provided in a typical implementation case of the present invention is in an open state; Figure 6 It is a structural schematic diagram of a second cover body of a sealing cover provided in a typical implementation case of the present invention in an open state; Figure 7 It is a structural schematic diagram of a second cover body of a sealing cover provided in a typical implementation case of the present invention in an open state; Figure 8 It is a schematic diagram of the back structure of a sealing cover in a closed state provided in a typical implementation case of the present invention; Fig. 9 It is a schematic cross-sectional structure diagram of a sealing cover in a closed state provided in a typical implementation case of the present invention. DETAILED DESCRIPTION

[0013] In view of the deficiencies in the prior art, the inventor of this case has proposed the technical solution of the present invention after long-term research and extensive practice. The technical solution, its implementation process and principle will be further explained as follows.

[0014] A first aspect of an embodiment of the present invention provides a sealing cover for balancing the pressure in multiple reagent chambers, comprising: a first cover body, a second cover body, x first sealing structures, a second sealing structure, and a third sealing structure, wherein the first cover body is used to be sealed and combined with a box body, and the second cover body is openably covered on the first cover body; The first cover body has a first surface, on which a mounting hole, a first operating hole and x air holes are arranged penetrating the first cover body, the x air holes are distributed around the first operating hole, and x first sealing structures are fixedly arranged on the first surface, each of the first sealing structures covers an air hole, and the first sealing structure is permeable to air but not permeable to liquid; the second cover body has a second surface, on which a second operating hole penetrating the second cover body is arranged, and a pressure column is also arranged on the second surface; When the second cover body is covered on the first cover body, the first cover body is sealed and combined with the second cover body, and the first surface and the second surface enclose a sandwich space isolated from the external environment, the first operating hole corresponds to and is connected with the second operating hole, and the pressure column corresponds to the assembly hole; the second sealing structure and the third sealing structure are arranged between the first surface and the second surface, the second sealing structure is arranged between the first operating hole and the x first sealing structures, the second sealing structure is arranged around the periphery of the first operating hole and the second operating hole, and the third sealing structure is arranged around the periphery of the x first sealing structures, the second sealing structure and the third sealing structure are sealed and matched with the first surface and the second surface, the second sealing structure, the third sealing structure, the first surface and the second surface jointly enclose a closed ventilation space, the x first sealing structures are encapsulated in the ventilation space, and the air vent can exchange gas with the ventilation space through the first sealing structure, x≥2.

[0015] Furthermore, the first sealing structure includes a sealing column, a sealing ring and a sieve plate, the sealing column is fixedly arranged on the first surface of the first cover body, the sealing column has a through air vent inside, the air vent is arranged around the air hole, the sealing ring and the sieve plate are arranged in the air vent, the sealing ring surrounds the air hole and is arranged between the sieve plate and the first cover body, the sieve plate is sealed with the first cover body via the sealing ring, the sieve plate covers the air hole, and the sieve plate is permeable to air but not to liquid.

[0016] Furthermore, a limiting structure is also arranged on the inner wall of the vent hole, the sieve plate and the sealing ring are arranged between the limiting structure and the first cover body, and the limiting structure is pressed on the sieve plate.

[0017] Furthermore, the sealing ring is a flexible component, and the sealing ring located between the sieve plate and the first cover body is in an elastically compressed state.

[0018] Furthermore, the second sealing structure and the third sealing structure are fixedly arranged on the first surface or the second surface, or one of the second sealing structure and the third sealing structure is fixedly arranged on the first surface and the other is fixed on the second surface, and the second sealing structure and the third sealing structure are both annular structures.

[0019] Furthermore, the second sealing structure and the third sealing structure both include a support ring and a flexible rubber coating distributed on the end surface of the support ring.

[0020] Furthermore, a positioning groove is provided on the second surface of the second cover body, the contour shape of the positioning groove is the same as the contour shape of the first cover body, the contour graphic area of ​​the positioning groove is larger than the contour graphic area of ​​the first cover body, the pressure column and the second operating hole are arranged at the bottom of the positioning groove, and when the second cover body is covered on the first cover body, at least a part of the first cover body close to the second cover body is arranged in the positioning groove.

[0021] Furthermore, the second sealing structure and the third sealing structure are arranged at the bottom of the positioning groove.

[0022] Furthermore, the first cover body is provided with y buckles, and the second cover body is provided with y slots, the y slots correspond to the y buckles one by one, and when the second cover body is covered on the first cover body, the y buckles are respectively inserted into the y slots, y≥1.

[0023] Furthermore, the card slot is communicated with the positioning slot.

[0024] Furthermore, the outer peripheral side surface of the second cover body is also provided with an extension block extending outward.

[0025] Furthermore, the second cover body is rotatably connected to the first cover body.

[0026] Furthermore, the second cover body is hinged or pivotally connected to the first cover body.

[0027] The second aspect of an embodiment of the present invention provides a test box, which includes: a box body and a sealing cover for balancing the pressure in multiple reagent chambers, the box body has x reagent chambers, a sample tube chamber and a negative pressure chamber, the x reagent chambers are isolated from each other, the sealing cover is arranged on the box body, the first cover body is sealed and combined with the box body, the x air holes correspond to the x reagent chambers one by one, the first operating hole corresponds to and is connected to the negative pressure chamber, and the assembly hole corresponds to and is connected to the sample tube chamber.

[0028] The technical solution, its implementation process and principles, etc. will be further explained below in conjunction with the accompanying drawings and specific implementation cases. Unless otherwise specified, the sieve plate, sealing ring, buckle, slot, etc. used in the present invention are all known in the art. The test box / sealing cover, etc. in the present invention can be manufactured by conventional means known in the art and are not specifically limited here.

[0029] See also Figure 1 A test box includes a box body 100 and a sealing cover 200. The box body 100 has x reagent chambers, a sample tube chamber and a negative pressure chamber. The x reagent chambers are isolated from each other. The x reagent chambers and the negative pressure chamber are arranged on one side of the sample tube chamber. The x reagent chambers surround the negative pressure chamber. The tops of the reagent chambers, the sample tube chambers and the negative pressure chamber are open chamber openings. The sealing cover 200 is arranged on the box body 100 and covers the chamber openings on the tops of the x reagent chambers, the sample tube chamber and the negative pressure chamber.

[0030] It should be noted that the sealing cover 200 can be fixedly combined with the box body 100 by a snap-fit ​​213 structure known in the art, and the sealing cover 200 is in sealing contact with at least the edges of the tops of the x reagent chambers, the sample tube chamber and the negative pressure chamber to ensure that the chamber openings between the x reagent chambers, the sample tube chamber and the negative pressure chamber are isolated from each other. As known to those skilled in the art, the reagent chamber is mainly used to hold reagents or waste liquids, the sample tube chamber is used to accommodate sample tubes, and the negative pressure chamber is mainly used to accommodate syringes or to be configured to form a negative pressure syringe.

[0031] In a typical implementation case, please refer to Figure 2-Figure 8The sealing cover 200 includes a first cover body 210 (which can be called a bottom cover / lower cover, the same below), a second cover body 220 (which can be called a top cover / upper cover, the same below), x first sealing structures 240 and a second sealing structure 250, and a third sealing structure 260. The first cover body 210 is fixedly provided on the box body 100 and is in sealing contact with the edges of the tops of the x reagent compartments, the sample tube compartments and the negative pressure compartments. The second cover body 220 is connected to the first cover body 210 via a flexible hinge 230. The second cover body 220 can be opened and covered on the first cover body 210. The first cover body 210 has a first surface, and an assembly hole 212, a first operation hole 211 and x air holes 214 are provided on the first surface, and the x air holes 214 are distributed around the first operation hole 211. The assembly hole 212 is correspondingly provided on the top of the sample tube compartment and is directly connected to the sample tube compartment. The first operation hole 211 is It should be arranged on the top of the negative pressure chamber and directly connected with the negative pressure chamber, x air holes 214 are respectively arranged on the tops of x reagent chambers and are respectively connected with the x reagent chambers one by one, x first sealing structures 240 are fixedly arranged on the first surface, each first sealing structure 240 covers an air hole 214, and the first sealing structure 240 is permeable to air but not permeable to liquid; the second cover body 220 has a second surface, and a second operating hole 221 penetrating the second cover body 220 is arranged on the second surface, and a pressure column 222 is also arranged on the second surface, the second sealing structure 250 and the third sealing structure 260 are fixedly arranged on the second surface, the second sealing structure 250 surrounds the second operating hole 221, and the third sealing structure 260 surrounds the second sealing structure 250, the second operating hole 221, the second sealing structure 250, and the third sealing structure 260 are arranged at intervals along their own radial directions, and x=7 shown in the figure, and x can of course also be other values ​​greater than 2.

[0032] Please also read Figure 6-Figure 9 When the second cover body 220 is covered on the first cover body 210, the first cover body 210 and the second cover body 220 are sealed and combined, and the first surface and the second surface are enclosed to form a sandwich space isolated from the external environment, the first operation hole 211 corresponds to and is connected with the second operation hole 221, and the pressure column 222 corresponds to the assembly hole 212; the second sealing structure 250 is arranged around the first operation hole 211 and the x first sealing structures 240, and the third sealing structure 260 is arranged around the periphery of the x first sealing structures 240. The second sealing structure 250 and the third sealing structure 260 are in sealing contact with the first surface of the first cover body 210. The second sealing structure 250, the third sealing structure 260, the first surface and the second surface are enclosed together to form a closed ventilation space, and the x first sealing structures 240 are encapsulated in the ventilation space. The air vent 214 can exchange gas with the ventilation space through the first sealing structure 240, and x≥2.

[0033] Specifically, the first cover body 210 of the sealing cover 200 is sealed with the box body 100. At the same time, the first cover body 210 is in sealing contact with the edges of the tops of the x reagent chambers, the sample tube chamber and the negative pressure chamber. The first sealing structure 240 is air-permeable but not liquid-permeable, forming a first seal for the reagent chamber. When the second cover body 220 is placed on the first cover body 210, the second sealing structure 250 and the third sealing structure 260 are in sealing contact with the first cover body 210 and the second cover body 220 to form a second seal. The x first sealing structures 240 are encapsulated in a closed ventilation space formed by the second sealing structure 250, the third sealing structure 260, the first surface and the second surface. Gas communication and ventilation can be achieved between the x reagent chambers via the first sealing structure 240 and the closed ventilation space. Specifically, when the syringe is connected to one of the reagent chambers for aspirating liquid, the negative pressure above the reagent chamber will be evenly distributed in the x reagent chambers; this ensures that the single reagent chamber is under less pressure, and when the syringe is connected to the waste liquid chamber among the x reagent chambers, the liquid in the syringe is discharged into the waste liquid chamber, and the pressure in other reagent chambers can also be kept balanced.

[0034] Specifically, the first sealing structure 240 includes a sealing column 241, a sealing ring 242 and a sieve plate 243. The sealing column 241 is fixedly arranged on the first surface of the first cover body 210. The sealing column 241 has a through vent 2411 inside. The vent 2411 is arranged around the vent 214. The sealing ring 242 and the sieve plate 243 are arranged in the vent 2411. The sealing ring 242 surrounds the vent 214 and is arranged between the sieve plate 243 and the first cover body 210. A limiting structure 2412 is also provided on the inner wall of the vent hole 2411, and the sieve plate 243 and the sealing ring 242 are arranged between the limiting structure 2412 and the first cover body 210. The limiting structure 2412 is pressed on the sieve plate 243, and the sealing ring 242 is tightly pressed between the sieve plate 243 and the first surface of the first cover body 210. The sieve plate 243 is sealed with the first cover body 210 via the sealing ring 242, and the sieve plate 243 covers the vent hole 214. The sieve plate 243 is permeable to air but not to liquid.

[0035] Exemplarily, the material of the sieve plate 243 is a PE polymer, which has certain stretching and deformation characteristics. The sealing ring 242 is a rubber O-ring, which also has stretching and deformation characteristics. When the sieve plate 243 and the sealing ring 242 are compressed between the limiting structure 2412 and the first cover plate, they deform themselves, thereby being tightly attached to the sealing column 241 or the first cover plate, thereby ensuring the sealing effect. It can be understood that the limiting structure 2412 on the inner wall of the vent 2411 is an annular protrusion that protrudes radially along the vent 2411. The distance between the limiting structure 2412 and the first surface of the first cover plate is less than the total thickness of the sieve plate 243 and the sealing ring 242 stacked together. Therefore, the sieve plate 243 and the sealing ring 242 located between the limiting structure 2412 and the first cover plate will remain in a compressed and deformed state, thereby improving the sealing effect between the sieve plate 243, the sealing ring 242 and the first cover plate and the sealing column 241. For details, please refer to Figure 3 , Figure 6 and Fig. 9 The second sealing structure 250 and the third sealing structure 260 are both annular structures, preferably an annular columnar structure. The second sealing structure 250 and the third sealing structure 260 are provided with a flexible rubber coating 270 on the end surface facing away from the second surface of the second cover body 220. When the second sealing structure 250 and the third sealing structure 260 are in contact with the first cover body 210, the flexible rubber coating 270 on the second sealing structure 250 and the third sealing structure 260 is deformed under pressure and maintains close contact with the first surface of the first cover body 210, thereby ensuring the airtightness of the formed ventilation space. Exemplarily, the second sealing structure 250 and the third sealing structure 260 may include a rigid support ring and a flexible rubber coating wrapped in the support ring. The rigid support ring may be a hard composite plastic component, etc., and the flexible rubber coating may be a rubber or polymer material, etc., which may be known in the art and are not specifically limited here.

[0036] Specifically, a first positioning groove 223 is provided on the second surface of the second cover body 220, and the contour shape of the first positioning groove 223 is the same as the contour shape of the first cover body 210 as a whole. The contour graphic area of ​​the first positioning groove 223 is larger than the contour graphic area of ​​the first cover body 210. The pressure column 222, the second operating hole 221, the second sealing structure 250, and the third sealing structure 260 are all arranged at the bottom of the first positioning groove 223. When the second cover body 220 is covered on the first cover body 210, at least the surface portion of the first cover body 210 close to the second cover body 220 is arranged in the first positioning groove 223.

[0037] Specifically, the first cover 210 is also provided with y buckles 213, and the second cover 220 is provided with y slots 224, and the y slots 224 correspond to the y buckles 213 one by one. When the second cover 220 is covered on the first cover 210, the y buckles 213 are respectively inserted into the y slots 224, and y=2 in the figure. As a preferred solution, the slot 224 can be connected with the first positioning slot 223. Of course, the slot 224 can also be independently arranged on the periphery of the first positioning slot 223. Specifically, the outer peripheral side of the second cover 220 is also provided with an extension block 225 extending outward. By providing the extension block 225, it is more convenient to perform the opening operation on the second cover 220. Preferably, the slot 224 can be correspondingly arranged in the extension block 225.

[0038] For details, please refer to Figure 7 and Figure 8 The first cover body 210 also has a third surface, which is arranged back to back with the first surface. A plurality of welding lines 215 are arranged on the third surface. The distribution and contour shapes of the plurality of welding lines 215 are the same or similar to the distribution and contour shapes of the openings of the reagent chamber, the sample tube chamber, and the negative pressure chamber in the box body 100. The first cover body 210 is welded to the top of the box body 100 through the welding lines 215, and is sealed with the edges of the openings of the reagent chamber, the sample tube chamber, and the negative pressure chamber.

[0039] Specifically, a second positioning groove 216 is also provided on the third surface of the first cover body 210. The contour shape of the second positioning groove 216 is the same as the contour shape of the top of the box body 100, but the area is slightly larger than the area of ​​the top of the box body 100. The top of the box body 100 is embedded in the second positioning groove 216 of the first cover body 210 and fits tightly with the groove wall of the second positioning groove 216, wherein structures such as the welding line 215 are distributed at the bottom of the second positioning groove 216.

[0040] It should be noted that, in addition to the above description, the structure and method for achieving fixed connection and sealing cooperation between the first cover body 210 and the box body 100 may also be other structures and methods known in the art. The covering between the first cover body 210 and the second cover body 220 and the matching and fitting at the edges after covering are all known in the art and are not specifically limited here.

[0041] A test kit provided by an embodiment of the present invention can place the entire system of the amplification reaction (including liquid) in each functional compartment for sealing operation, thereby avoiding contamination of the operating space environment, reducing false positives, and improving detection accuracy; a sealing cover in a test kit provided by an embodiment of the present invention has double sealing protection, reduces the risk of liquid leakage, and can ensure safety in different transportation environments. In addition, a sealing cover in a test kit provided by an embodiment of the present invention has a simple structure, does not require hot pressing, and can protect reagents or freeze-dried balls that are sensitive to temperature.

[0042] It should be understood that the above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A sealing cover for balancing the pressure in multiple reagent chambers, characterized in that: include: A first cover, a second cover, x first sealing structures, a second sealing structure, and a third sealing structure, wherein the first cover is used for sealingly combining with the box body, and the second cover is openably covered on the first cover; The first cover body has a first surface, on which a mounting hole, a first operating hole and x air holes are arranged penetrating the first cover body, the x air holes are distributed around the first operating hole, and x first sealing structures are fixedly arranged on the first surface, each of the first sealing structures covers an air hole, and the first sealing structure is permeable to air but not permeable to liquid; the second cover body has a second surface, on which a second operating hole penetrating the second cover body is arranged, and a pressure column is also arranged on the second surface; When the second cover body is covered on the first cover body, the first cover body is sealed and combined with the second cover body, and the first surface and the second surface enclose a sandwich space isolated from the external environment, the first operating hole corresponds to and is connected with the second operating hole, and the pressure column corresponds to the assembly hole; the second sealing structure and the third sealing structure are arranged between the first surface and the second surface, the second sealing structure is arranged between the first operating hole and the x first sealing structures, the second sealing structure is arranged around the periphery of the first operating hole and the second operating hole, and the third sealing structure is arranged around the periphery of the x first sealing structures, the second sealing structure and the third sealing structure are sealed and matched with the first surface and the second surface, the second sealing structure, the third sealing structure, the first surface and the second surface jointly enclose a closed ventilation space, the x first sealing structures are encapsulated in the ventilation space, and the air vent can exchange gas with the ventilation space through the first sealing structure, x≥2.

2. The sealing cover for balancing the pressure in multiple reagent chambers according to claim 1, characterized in that: The first sealing structure includes a sealing column, a sealing ring and a sieve plate. The sealing column is fixedly arranged on the first surface of the first cover body. The sealing column has a through vent hole inside. The vent hole is arranged around the vent hole. The sealing ring and the sieve plate are arranged in the vent hole. The sealing ring surrounds the vent hole and is arranged between the sieve plate and the first cover body. The sieve plate is sealed with the first cover body via the sealing ring. The sieve plate covers the vent hole. The sieve plate is permeable to air but not to liquid.

3. The sealing cover for balancing the pressure in multiple reagent chambers according to claim 2, characterized in that: A limiting structure is also arranged on the inner wall of the vent hole, the sieve plate and the sealing ring are arranged between the limiting structure and the first cover body, and the limiting structure is pressed on the sieve plate.

4. The sealing cover for balancing the pressure in multiple reagent chambers according to claim 2, characterized in that: The sealing ring is a flexible component, and the sealing ring located between the sieve plate and the first cover body is in an elastically compressed state.

5. The sealing cover for balancing the pressure in multiple reagent chambers according to claim 2, characterized in that: The second sealing structure and the third sealing structure are fixedly arranged on the first surface or the second surface, or one of the second sealing structure and the third sealing structure is fixedly arranged on the first surface and the other is fixedly arranged on the second surface, and the second sealing structure and the third sealing structure are both annular structures.

6. The sealing cover for balancing the pressure in multiple reagent chambers according to claim 5, characterized in that: The second sealing structure and the third sealing structure both include a support ring and a flexible rubber coating distributed on the end surface of the support ring.

7. The sealing cover for balancing the pressure in multiple reagent chambers according to any one of claims 1 to 5, characterized in that: The second surface of the second cover body is provided with a positioning groove, the contour shape of the positioning groove is the same as the contour shape of the first cover body, the contour pattern area of ​​the positioning groove is larger than the contour pattern area of ​​the first cover body, the pressure column and the second operating hole are arranged at the groove bottom of the positioning groove, and when the second cover body is covered on the first cover body, at least a part of the first cover body close to the second cover body is arranged in the positioning groove; And / or, the second sealing structure and the third sealing structure are arranged at the bottom of the positioning groove.

8. The sealing cover for balancing the pressure in multiple reagent chambers according to claim 7, characterized in that: The first cover body is also provided with y buckles, and the second cover body is provided with y slots, the y slots correspond to the y buckles one by one, and when the second cover body is covered on the first cover body, the y buckles are respectively inserted into the y slots, y≥1; And / or, the card slot is communicated with the positioning slot.

9. The sealing cover for balancing the pressure in multiple reagent chambers according to claim 1, characterized in that: The outer peripheral side surface of the second cover body is also provided with an extension block extending outward; and / or, the second cover body is rotatably connected to the first cover body; And / or, the second cover body is hinged or pivotally connected to the first cover body.

10. A test kit, characterized in that: include: A box body and a sealing cover for balancing the pressures in multiple reagent chambers as described in any one of claims 1 to 9, wherein the box body has x reagent chambers, a sample tube chamber and a negative pressure chamber, the x reagent chambers are isolated from each other, the sealing cover is provided on the box body, the first cover body is sealed and combined with the box body, the x air holes correspond one-to-one to the x reagent chambers respectively, the first operating hole corresponds to and is connected to the negative pressure chamber, and the assembly hole corresponds to and is connected to the sample tube chamber.

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