Antigen-antibody reaction device and method

Abstract

The invention discloses an antigen-antibody reaction device and method, and belongs to the field of experimental appliances, the device comprises a box body, and also comprises a door plate slidably connected with the box body through a slide rail; the swinging mechanism is arranged at the bottom in the box body, and a perforated plate is arranged at the top of the swinging mechanism; the two first connecting plates are arranged at the two ends of the top of the interior of the box body, a conveying mechanism is arranged on the first connecting plates, the conveying mechanism comprises upper rods, lower rods and a placing plate, and one ends of the two upper rods are rotationally connected with the tops of one sides of the two first connecting plates correspondingly; one ends of the two lower rods are rotationally connected with the bottoms of one sides of the two first connecting plates correspondingly; the other end of the upper rod and the other end of the lower rod are rotationally connected with the placing plate; the dropper is arranged on the placing plate; the extrusion mechanism is arranged at the top end of the box body in a sliding and penetrating manner. The droppers are conveyed into the box body through the conveying mechanism, the droppers are extruded through the extrusion mechanism, and automation of the reactant adding process is achieved.

Description

Technical Field

[0001] The invention belongs to the field of experimental tools, and in particular relates to an antigen-antibody reaction device and method. Background Art

[0002] Antigen-antibody reaction is the process in which an antigen and a corresponding antibody specifically bind to form a reversible antigen-antibody complex under certain conditions. This reaction is based on the structural complementarity and affinity between the antigenic determinant and the antibody hypervariable region molecules, and is determined by the spatial configuration of the antigen and antibody molecules. Antigen-antibody reaction has the characteristics of specificity, proportionality, reversibility and stage, and is of great clinical significance, such as being used to diagnose or assist in the diagnosis of related diseases, detect or identify pathogenic microorganisms, etc. With the continuous deepening of biomedical and immunological research, antigen-antibody reaction is increasingly widely used in disease diagnosis, vaccine development, drug screening and other fields. Therefore, it is particularly important to develop a device that can automatically perform antigen-antibody reaction and improve experimental efficiency and accuracy.

[0003] The current antigen-antibody reaction requires operators to manually mix the antigen and antibody, and the reaction process is not automated enough. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the disadvantages of the above-mentioned prior art and provide an antigen-antibody reaction device and method.

[0005] The technical solution adopted to solve the above technical problems is: an antigen-antibody reaction device, including a box body, and also including:

[0006] A door panel, wherein the door panel is slidably connected to the box body via a slide rail;

[0007] A swing mechanism, the swing mechanism is arranged at the bottom of the box body, a porous plate is arranged on the top of the swing mechanism, and a plurality of blind holes are evenly arranged on the porous plate;

[0008] A first connecting plate, two of the first connecting plates are arranged at two ends of the top of the box body, a transport mechanism is arranged on the first connecting plate, and the transport mechanism includes an upper rod, a lower rod, a second connecting plate, and a placement plate, one end of the two upper rods are respectively connected to the top of one side of the two first connecting plates for rotation; one end of the two lower rods are respectively connected to the bottom of one side of the two first connecting plates for rotation; the other end of the upper rod and the other end of the lower rod are both connected to the second connecting plate for rotation, and the two second connecting plates are connected to the placement plates, and a plurality of through holes are evenly arranged on the placement plates;

[0009] A dropper, the dropper being arranged on the placement plate;

[0010] The squeezing mechanism is slidably penetrated and arranged on the top of the box body.

[0011] Through the above technical solution, the transport mechanism transports the dropper into the box, and the extrusion mechanism squeezes the dropper, thereby realizing the automation of the process of adding reactants; during the reaction process, the door panel is closed and the box is sealed, reducing the impact of the outside world on the reaction process.

[0012] Further, as a preferred embodiment of the present invention, the swing mechanism includes:

[0013] A bottom plate, the bottom plate being rotatably disposed at the bottom end of the box body;

[0014] Connecting columns, four of which are arranged around the bottom plate, each two adjacent connecting columns form a group, and a fixing plate is arranged between each group of connecting columns;

[0015] A movable plate, the movable plate being rotatably disposed between two fixed plates;

[0016] A central axis, the middle portion of which is rotatably connected to the movable plate, and both ends of the central axis are provided with bent plates, one of which is rotatably connected to the side wall of the box body via a rotating shaft;

[0017] A third connecting plate is disposed on the connecting column and connected to the porous plate.

[0018] Through the above technical solution, the multi-well plate is shaken during the reaction process to fully mix the antigen and antibody.

[0019] Furthermore, as a preferred embodiment of the present invention, a second motor is connected to the other bent plate.

[0020] Through the above technical solution, the second motor drives the central shaft to rotate, thereby driving the porous plate to reciprocate.

[0021] Further, as a preferred embodiment of the present invention, the third connecting plate is provided with a plurality of grooves;

[0022] The bottom of the porous plate is provided with positioning pins whose number is equal to the number of grooves on the third connecting plate, the positioning pins are provided with springs, and the ends of the springs are provided with clamping blocks.

[0023] Through the above technical solution, the connection between the porous plate and the third connecting plate is made more secure, preventing the porous plate from falling off during shaking.

[0024] Furthermore, as a preferred embodiment of the present invention, a first motor is provided in the box, and the first motor is connected to one of the lower rods.

[0025] Through the above technical solution, the first motor drives the lower rod to drive the placement plate to move.

[0026] Further, as a preferred embodiment of the present invention, the extrusion mechanism includes:

[0027] A cylinder, wherein the cylinder is arranged at the middle of the top of the box body, and the telescopic shaft of the cylinder penetrates into the interior of the box body;

[0028] A pressing plate connected to the telescopic shaft of the cylinder;

[0029] Guide shafts, two guide shafts are arranged through the top of the box body and are respectively located on both sides of the cylinder.

[0030] Through the above technical solution, the cylinder drives the pressing plate to squeeze out the reactants in the dropper.

[0031] Further, as a preferred embodiment of the present invention, the door panel is provided with an observation window;

[0032] The door panel is provided with a handle;

[0033] The bottom of the box body is provided with supporting feet;

[0034] The supporting feet are provided with gaskets.

[0035] Through the above technical solution, the reaction process can be better observed; the door panel can be easily opened and closed; and the box can be placed more stably.

[0036] Furthermore, as a preferred embodiment of the present invention, a heating ring is provided at the bottom of the box.

[0037] Through the above technical solution, the temperature inside the box can be controlled.

[0038] Furthermore, as a preferred embodiment of the present invention, an ultraviolet lamp is provided inside the box.

[0039] Through the above technical solution, the device can be easily disinfected after use.

[0040] The present invention also provides a reaction method of an antigen-antibody reaction device, the method comprising:

[0041] The dropper containing the antibody is placed on the placement plate, added into the multi-well plate through the squeezing mechanism, and waited for 3-5 minutes;

[0042] The dropper containing the antibody is replaced with a dropper containing the labeled substrate and added into the multi-well plate through a squeezing mechanism;

[0043] The presence and content of the antigen can be determined based on the color depth of the substrate after the reaction.

[0044] The beneficial effects of the present invention are as follows: the present invention transports the dropper into the box through the transport mechanism, and the extrusion mechanism squeezes the dropper to realize the automation of the process of adding reactants. During the reaction process, the box is closed, reducing the influence of the outside world on the reaction process. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 It is a schematic diagram of the three-dimensional structure of an antigen-antibody reaction device of the present invention;

[0046] Figure 2 It is a schematic diagram of the three-dimensional structure of the bottom of an antigen-antibody reaction device of the present invention;

[0047] Figure 3 It is a three-dimensional structural schematic diagram of an extrusion mechanism of an antigen-antibody reaction device of the present invention;

[0048] Figure 4 It is a three-dimensional structural schematic diagram of a transport mechanism of an antigen-antibody reaction device of the present invention;

[0049] Figure 5 It is a three-dimensional structural schematic diagram of a swing mechanism of an antigen-antibody reaction device of the present invention;

[0050] Figure 6 It is a schematic diagram of the three-dimensional structure of the bottom of a multi-well plate of an antigen-antibody reaction device of the present invention;

[0051] Figure 7 It is a partial enlarged view of location A of a multi-well plate of an antigen-antibody reaction device of the present invention.

[0052] Figure numerals: 1. Box body; 11. Support foot; 12. Gasket; 13. Slide rail; 14. Heating coil; 2. Door panel; 21. Observation window; 22. Handle; 3. Transport mechanism; 31. First connecting plate; 32. Upper rod; 33. Lower rod; 34. Placement plate; 35. Second connecting plate; 36. First motor; 4. Extrusion mechanism; 41. Cylinder; 42. Press plate; 43. Guide shaft; 5. Perforated plate; 51. Locating pin; 52. Spring; 53. Block; 54. Third connecting plate; 55. Bending plate; 56. Rotating shaft; 57. Connecting column; 58. Fixed plate; 59. Movable plate; 60. Middle axis; 61. Bottom plate; 62. Second motor; 6. Dropper. DETAILED DESCRIPTION

[0053] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0054] like Figure 1As shown, an antigen-antibody reaction device of this embodiment includes a box 1 and also includes:

[0055] A door panel 2, wherein the door panel 2 is slidably connected to the box body 1 via a slide rail 13;

[0056] A swing mechanism, the swing mechanism is arranged at the bottom of the box body 1, a porous plate 5 is arranged on the top of the swing mechanism, and a plurality of blind holes are evenly arranged on the porous plate 5;

[0057] A first connecting plate 31, two of the first connecting plates 31 are arranged at both ends of the top of the box body 1, and a transport mechanism 3 is arranged on the first connecting plate 31, and the transport mechanism 3 includes an upper rod 32, a lower rod 33, a second connecting plate 35, and a placement plate 34, one end of the two upper rods 32 is rotatably connected to the top of one side of the two first connecting plates 31; one end of the two lower rods 33 is rotatably connected to the bottom of one side of the two first connecting plates 31; the other end of the upper rod 32 and the other end of the lower rod 33 are both rotatably connected to the second connecting plate 35, and the two second connecting plates 35 are connected with a placement plate 34, and a plurality of through holes are evenly arranged on the placement plate 34;

[0058] A dropper 6, the dropper 6 is arranged on the placement plate 34;

[0059] The squeezing mechanism 4 is slidably disposed on the top of the box body 1 .

[0060] Specifically, the working process of the transport mechanism 3 is as follows: the upper rod 32 and the lower rod 33 rotate synchronously to drive the placement plate 34 on the second connecting plate 35 to move from the inside of the box body 1 to the outside of the box body 1, and the placement plate 34 always remains parallel to the porous plate 5 during the movement.

[0061] Furthermore, when in use, the specific steps are: open the door panel 2, place the porous plate 5 containing the antigen into the box body 1, move the placement plate 34 to the outside of the box body 1, place the dropper 6 containing the antibody on the placement plate 34, move the placement plate 34 back into the box body 1, close the door panel 2, squeeze the dropper 6 by the squeezing mechanism 4 to make the antibody drip onto the porous plate 5, wait for the reaction, open the door panel 2, move the placement plate 34 to the outside of the box body 1, replace the dropper 6 containing the antibody with the dropper 6 containing the labeled substrate, move the placement plate 34 back into the box body 1, close the door panel 2, squeeze the dropper 6 by the squeezing mechanism 4 to make the labeled substrate drip onto the porous plate 5, and observe the color depth of the labeled substrate to determine the presence and content of the antigen.

[0062] Furthermore, the top of the dropper 6 is made of rubber material, which is convenient for squeezing out the antibody or labeled substrate stored in the dropper 6 .

[0063] like Figure 5 As shown, as a specific embodiment of the present invention, the swing mechanism includes:

[0064] A bottom plate 61, the bottom plate 61 is rotatably disposed at the bottom end of the box body 1;

[0065] Connecting columns 57, four connecting columns 57 are arranged around the bottom plate 61, and every two adjacent connecting columns 57 form a group, and a fixing plate 58 is arranged between each group of connecting columns 57;

[0066] A movable plate 59, the movable plate 59 is rotatably disposed between the two fixed plates 58;

[0067] A central axis 60, wherein the middle portion of the central axis 60 is rotatably connected to the movable plate 59, and both ends of the central axis 60 are provided with bent plates 55, wherein one of the bent plates 55 is rotatably connected to the side wall of the box body 1 via a rotating shaft 56;

[0068] The third connecting plate 54 is disposed on the connecting column 57 and connected to the porous plate 5 .

[0069] Specifically, the rotation of the rotating shaft 56 drives the bent plate 55 to rotate, and the bent plate 55 drives the central shaft 60 to rotate around the middle of the central shaft 60, drives the movable plate 59 to rotate between the two fixed plates 58, and drives the third connecting plate 54 and the porous plate 5 to reciprocate simultaneously.

[0070] like Figure 5 As shown, as a specific embodiment of the present invention, a second motor 62 is connected to the other bent plate 55 .

[0071] Furthermore, after adding the antibody, the second motor 62 is started to drive the swing mechanism to move, so that the antigen and antibody in the porous plate 5 are fully mixed.

[0072] like Figure 5-Figure 7 As shown, as a specific embodiment of the present invention, the third connecting plate 54 is provided with a plurality of grooves;

[0073] The bottom of the porous plate 5 is provided with positioning pins 51 whose number is equal to the number of grooves on the third connecting plate 54 . The positioning pins 51 are provided with springs 52 . The ends of the springs 52 are provided with clamping blocks 53 .

[0074] Specifically, when placing the porous plate 5, the positioning pin 51 is aligned with the groove. When the block 53 enters the groove, the spring 52 is compressed, making the connection between the porous plate 5 and the third connecting plate 54 more stable; when removing the porous plate 5, the spring 52 returns to its original state and the block 53 pops out.

[0075] like Figure 4 As shown, as a specific embodiment of the present invention, a first motor 36 is provided in the box body 1 , and the first motor 36 is connected to one of the lower rods 33 .

[0076] Specifically, the first motor 36 is a servo motor.

[0077] Furthermore, when the placement plate 34 moves to two extreme positions, namely, inside the box body 1 and outside the box body 1 , the first motor 36 plays a limiting role.

[0078] like Figure 3 As shown, as a specific embodiment of the present invention, the extrusion mechanism 4 includes:

[0079] A cylinder 41, wherein the cylinder 41 is disposed in the middle of the top of the box body 1, and a telescopic shaft of the cylinder 41 penetrates into the interior of the box body 1;

[0080] A pressing plate 42, wherein the pressing plate 42 is connected to the telescopic shaft of the cylinder 41;

[0081] The guide shafts 43 are two guide shafts 43 that penetrate through the top of the box body 1 and are located at both sides of the cylinder 41 respectively.

[0082] Specifically, the cylinder 41 drives the pressing plate 42 to perform linear motion, and the pressing plate 42 squeezes the dropper 6 to make the antibody or the labeled substrate in the dropper 6 drip out.

[0083] Furthermore, the stroke of the cylinder 41 can be set according to the required amount of the antibody or the labeled substrate.

[0084] like Figure 1 , Figure 2 As shown, as a specific embodiment of the present invention, the door panel 2 is provided with an observation window 21;

[0085] The door panel 2 is provided with a handle 22;

[0086] The bottom of the box body 1 is provided with a support foot 11;

[0087] A gasket 12 is provided on the support leg 11 .

[0088] Specifically, when opening or closing the door panel 2 , an operator can use the handle 22 for easy operation.

[0089] Furthermore, the legs 11 stabilize the box 1 when the box 1 is placed, and the gasket 12 is used for leveling to prevent the box 1 from shaking.

[0090] like Figure 1 As shown, as a specific embodiment of the present invention, a heating ring 14 is provided at the bottom of the box body 1.

[0091] Specifically, during the preparation process, the heating coil 14 is powered on to make the internal temperature of the box body 1 reach 37 degrees Celsius to achieve the best reaction conditions.

[0092] As a specific implementation of the present invention, an ultraviolet lamp is provided inside the box 1.

[0093] Specifically, after the reaction is finished, the porous plate 5 is taken out, the door panel 2 is closed and the ultraviolet lamp is turned on for a duration of not less than thirty minutes.

[0094] The present invention also provides a reaction method of an antigen-antibody reaction device, the method comprising:

[0095] The dropper 6 containing the antibody places the antibody on the placement plate 34, adds it into the porous plate 5 through the squeezing mechanism 4, and waits for 3-5 minutes;

[0096] The dropper 6 containing the antibody is replaced with the dropper 6 containing the labeled substrate and added into the multi-well plate 6 through the squeezing mechanism 4;

[0097] The presence and content of the antigen can be determined based on the color depth of the substrate after the reaction.

[0098] Specifically, the process of the method in the device is as follows: open the door panel 2, place the porous plate 5 containing the antigen into the box 1, move the placement plate 34 to the outside of the box 1, place the dropper 6 containing the antibody on the placement plate 34, move the placement plate 34 back into the box 1, close the door panel 2, squeeze the dropper 6 by the squeezing mechanism 4 to make the antibody drip onto the porous plate 5, wait for the reaction, open the door panel 2, move the placement plate 34 to the outside of the box 1, replace the dropper 6 containing the antibody with the dropper 6 containing the labeled substrate, move the placement plate 34 back into the box 1, close the door panel 2, squeeze the dropper 6 by the squeezing mechanism 4 to make the labeled substrate drip onto the porous plate 5, and observe the color depth of the labeled substrate after the reaction to determine the presence and content of the antigen.

[0099] The above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention.

Claims

1. An antigen-antibody reaction device, comprising a housing (1), characterized in that: Also includes: A door panel (2), wherein the door panel (2) is slidably connected to the box body (1) via a slide rail (13); A swing mechanism, the swing mechanism being arranged at the bottom of the box body (1), a porous plate (5) being arranged on the top of the swing mechanism, and a plurality of blind holes being evenly arranged on the porous plate (5); A first connecting plate (31), wherein the two first connecting plates (31) are arranged at two ends of the top of the box body (1), and a transport mechanism (3) is arranged on the first connecting plate (31), wherein the transport mechanism (3) comprises an upper rod (32), a lower rod (33), a second connecting plate (35), and a placement plate (34), wherein one end of the two upper rods (32) is rotatably connected to the top of one side of the two first connecting plates (31); one end of the two lower rods (33) is rotatably connected to the bottom of one side of the two first connecting plates (31); the other end of the upper rod (32) and the other end of the lower rod (33) are both rotatably connected to the second connecting plate (35), and the two second connecting plates (35) are connected to a placement plate (34), and the placement plate (34) is evenly provided with a plurality of through holes; A dropper (6), wherein the dropper (6) is arranged on the placement plate (34); A squeezing mechanism (4), wherein the squeezing mechanism (4) is slidably penetrated and arranged at the top end of the box body (1).

2. An antigen-antibody reaction device according to claim 1, characterized in that: The swing mechanism comprises: A bottom plate (61), the bottom plate (61) being rotatably disposed at the bottom end of the box body (1); Connecting columns (57), four of which are arranged around the bottom plate (61), Every two adjacent connecting columns (57) form a group, and a fixing plate (58) is provided between each group of connecting columns (57); A movable plate (59), wherein the movable plate (59) is rotatably disposed between the two fixed plates (58); A central axis (60), wherein the middle portion of the central axis (60) is rotatably connected to the movable plate (59), and bent plates (55) are provided at both ends of the central axis (60), wherein one of the bent plates (55) is rotatably connected to the side wall of the box body (1) via a rotating shaft (56); A third connecting plate (54), the third connecting plate (54) is arranged on the connecting column (57) and is connected to the porous plate (5).

3. An antigen-antibody reaction device according to claim 2, characterized in that: A second motor (62) is connected to the other bent plate (55).

4. An antigen-antibody reaction device according to claim 2, characterized in that: The third connecting plate (54) is provided with a plurality of grooves; The bottom of the porous plate (5) is provided with positioning pins (51) whose number is equal to the number of grooves on the third connecting plate (54); a spring (52) is provided on the positioning pin (51); and a clamping block (53) is provided at the end of the spring (52).

5. The antigen-antibody reaction device according to claim 1, characterized in that: A first motor (36) is provided in the box body (1), and the first motor (36) is connected to one of the lower rods (33).

6. The antigen-antibody reaction device according to claim 1, characterized in that: The extrusion mechanism (4) comprises: The cylinder (41) is arranged at the middle of the top of the box (1). The telescopic shaft of the cylinder (41) penetrates into the interior of the box (1); A pressing plate (42), the pressing plate (42) being connected to the telescopic shaft of the cylinder (41); Guide shafts (43), two guide shafts (43) are arranged through the top of the box body (1) and are respectively located on both sides of the cylinder (41).

7. The antigen-antibody reaction device according to claim 1, characterized in that: The door panel (2) is provided with an observation window (21); The door panel (2) is provided with a handle (22); The bottom of the box body (1) is provided with supporting feet (11); A gasket (12) is provided on the supporting foot (11).

8. An antigen-antibody reaction device according to claim 1, characterized in that A heating ring (14) is provided at the bottom of the box (1).

9. The antigen-antibody reaction device according to claim 1, characterized in that: An ultraviolet lamp is arranged inside the box (1).

10. The reaction method of the antigen-antibody reaction device according to any one of claims 1 to 9, characterized in that: The method comprises: The antibody is placed on the placement plate (34) by a dropper (6) containing the antibody, added into the porous plate (5) through the squeezing mechanism (4), and waited for 3-5 minutes; The dropper (6) containing the antibody is replaced with a dropper (6) containing the labeled substrate and added into the multi-well plate (6) through the squeezing mechanism (4); The presence and content of the antigen can be determined based on the color depth of the substrate after the reaction.

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